Mediates both low-affinity uptake and efflux of sugar across the membrane
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 57, 687 |
| AclWI | GGATC | 1 cut(s) | 119 |
| AcuI | CTGAAG | 1 cut(s) | 264 |
| AgsI | TTSAA | 4 cut(s) | 154, 439, 541, 635 |
| AhlI | ACTAGT | 1 cut(s) | 325 |
| AluBI | AGCT | 3 cut(s) | 119, 247, 456 |
| AluI | AGCT | 3 cut(s) | 119, 247, 456 |
| Alw21I | GWGCWC | 1 cut(s) | 9 |
| Alw26I | GTCTC | 1 cut(s) | 614 |
| Alw44I | GTGCAC | 1 cut(s) | 5 |
| AlwI | GGATC | 1 cut(s) | 119 |
| Ama87I | CYCGRG | 1 cut(s) | 371 |
| ApaLI | GTGCAC | 1 cut(s) | 5 |
| ApeKI | GCWGC | 1 cut(s) | 350 |
| AseI | ATTAAT | 2 cut(s) | 192, 222 |
| AsuHPI | GGTGA | 1 cut(s) | 208 |
| AvaI | CYCGRG | 1 cut(s) | 371 |
| BaeGI | GKGCMC | 1 cut(s) | 9 |
| BbsI | GAAGAC | 1 cut(s) | 687 |
| Bbv12I | GWGCWC | 1 cut(s) | 9 |
| BbvI | GCAGC | 1 cut(s) | 337 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 592, 626 |
| BcoDI | GTCTC | 1 cut(s) | 614 |
| BcuI | ACTAGT | 1 cut(s) | 325 |
| BfaI | CTAG | 3 cut(s) | 11, 18, 326 |
| BisI | GCNGC | 1 cut(s) | 351 |
| BlsI | GCNGC | 1 cut(s) | 352 |
| BmeT110I | CYCGRG | 1 cut(s) | 371 |
| BmsI | GCATC | 1 cut(s) | 4 |
| BpiI | GAAGAC | 1 cut(s) | 687 |
| BpmI | CTGGAG | 1 cut(s) | 645 |
| BpuEI | CTTGAG | 1 cut(s) | 493 |
| BseRI | GAGGAG | 1 cut(s) | 450 |
| BseSI | GKGCMC | 1 cut(s) | 9 |
| BseXI | GCAGC | 1 cut(s) | 337 |
| BsiHKAI | GWGCWC | 1 cut(s) | 9 |
| BsiHKCI | CYCGRG | 1 cut(s) | 371 |
| BsmAI | GTCTC | 1 cut(s) | 614 |
| BsoBI | CYCGRG | 1 cut(s) | 371 |
| Bsp1286I | GDGCHC | 1 cut(s) | 9 |
| Bsp143I | GATC | 1 cut(s) | 124 |
| BspACI | CCGC | 2 cut(s) | 57, 687 |
| BspPI | GGATC | 1 cut(s) | 119 |
| BssMI | GATC | 1 cut(s) | 124 |
| Bst4CI | ACNGT | 3 cut(s) | 148, 158, 608 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 508 |
| BstC8I | GCNNGC | 1 cut(s) | 59 |
| BstKTI | GATC | 1 cut(s) | 127 |
| BstMAI | GTCTC | 1 cut(s) | 614 |
| BstMBI | GATC | 1 cut(s) | 124 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 347, 508, 695 |
| BstNSI | RCATGY | 1 cut(s) | 511 |
| BstSLI | GKGCMC | 1 cut(s) | 9 |
| BstV1I | GCAGC | 1 cut(s) | 337 |
| BstV2I | GAAGAC | 1 cut(s) | 687 |
| BtsIMutI | CAGTG | 1 cut(s) | 153 |
| Cac8I | GCNNGC | 1 cut(s) | 59 |
| CviAII | CATG | 2 cut(s) | 448, 508 |
| CviJI | RGCY | 8 cut(s) | 61, 119, 247, 341, 350, 407, 456, 698 |
| CviKI_1 | RGCY | 8 cut(s) | 61, 119, 247, 341, 350, 407, 456, 698 |
| DpnI | GATC | 1 cut(s) | 126 |
| DpnII | GATC | 1 cut(s) | 124 |
| DraI | TTTAAA | 1 cut(s) | 487 |
| Eco57I | CTGAAG | 1 cut(s) | 264 |
| Eco88I | CYCGRG | 1 cut(s) | 371 |
| FaeI | CATG | 2 cut(s) | 451, 511 |
| FatI | CATG | 2 cut(s) | 447, 507 |
| FauI | CCCGC | 2 cut(s) | 50, 694 |
| Fnu4HI | GCNGC | 1 cut(s) | 351 |
| Fsp4HI | GCNGC | 1 cut(s) | 351 |
| FspBI | CTAG | 3 cut(s) | 11, 18, 326 |
| GluI | GCNGC | 1 cut(s) | 351 |
| GsuI | CTGGAG | 1 cut(s) | 645 |
| Hin1II | CATG | 2 cut(s) | 451, 511 |
| HindIII | AAGCTT | 1 cut(s) | 117 |
| HinfI | GANTC | 1 cut(s) | 391 |
| HphI | GGTGA | 1 cut(s) | 208 |
| Hpy166II | GTNNAC | 3 cut(s) | 7, 323, 583 |
| Hpy188III | TCNNGA | 1 cut(s) | 472 |
| Hpy8I | GTNNAC | 3 cut(s) | 7, 323, 583 |
| HpyCH4III | ACNGT | 3 cut(s) | 148, 158, 608 |
| HpyCH4IV | ACGT | 1 cut(s) | 83 |
| HpyCH4V | TGCA | 7 cut(s) | 7, 143, 353, 502, 617, 650, 712 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 347, 508, 695 |
| HpySE526I | ACGT | 1 cut(s) | 83 |
| Hsp92II | CATG | 2 cut(s) | 451, 511 |
| Kzo9I | GATC | 1 cut(s) | 124 |
| LmnI | GCTCC | 1 cut(s) | 626 |
| LpnPI | CCDG | 4 cut(s) | 39, 242, 538, 609 |
| Lsp1109I | GCAGC | 1 cut(s) | 337 |
| LweI | GCATC | 1 cut(s) | 4 |
| MaeI | CTAG | 3 cut(s) | 11, 18, 326 |
| MaeII | ACGT | 1 cut(s) | 83 |
| MaeIII | GTNAC | 4 cut(s) | 170, 214, 234, 428 |
| MalI | GATC | 1 cut(s) | 126 |
| MboI | GATC | 1 cut(s) | 124 |
| MboII | GAAGA | 3 cut(s) | 259, 397, 692 |
| MfeI | CAATTG | 1 cut(s) | 635 |
| MhlI | GDGCHC | 1 cut(s) | 9 |
| MluCI | AATT | 4 cut(s) | 228, 414, 630, 635 |
| MmeI | TCCRAC | 1 cut(s) | 19 |
| MnlI | CCTC | 4 cut(s) | 291, 396, 455, 471 |
| MseI | TTAA | 3 cut(s) | 192, 222, 486 |
| MslI | CAYNNNNRTG | 1 cut(s) | 12 |
| MspA1I | CMGCKG | 1 cut(s) | 689 |
| MunI | CAATTG | 1 cut(s) | 635 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 347, 508, 695 |
| NdeII | GATC | 1 cut(s) | 124 |
| NlaIII | CATG | 2 cut(s) | 451, 511 |
| NmuCI | GTSAC | 2 cut(s) | 214, 428 |
| NspI | RCATGY | 1 cut(s) | 511 |
| PfeI | GAWTC | 1 cut(s) | 391 |
| PkrI | GCNGC | 1 cut(s) | 352 |
| PshBI | ATTAAT | 2 cut(s) | 192, 222 |
| RseI | CAYNNNNRTG | 1 cut(s) | 12 |
| SaqAI | TTAA | 3 cut(s) | 192, 222, 486 |
| SatI | GCNGC | 1 cut(s) | 351 |
| Sau3AI | GATC | 1 cut(s) | 124 |
| SduI | GDGCHC | 1 cut(s) | 9 |
| SetI | ASST | 9 cut(s) | 12, 23, 82, 86, 121, 136, 141, 249, 458 |
| SfaNI | GCATC | 1 cut(s) | 4 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 12 |
| SmlI | CTYRAG | 1 cut(s) | 472 |
| SmoI | CTYRAG | 1 cut(s) | 472 |
| SpeI | ACTAGT | 1 cut(s) | 325 |
| Sse9I | AATT | 4 cut(s) | 228, 414, 630, 635 |
| SsiI | CCGC | 2 cut(s) | 57, 687 |
| SspMI | CTAG | 3 cut(s) | 11, 18, 326 |
| TaaI | ACNGT | 3 cut(s) | 148, 158, 608 |
| TaiI | ACGT | 1 cut(s) | 86 |
| TaqI | TCGA | 2 cut(s) | 123, 394 |
| TasI | AATT | 4 cut(s) | 228, 414, 630, 635 |
| TfiI | GAWTC | 1 cut(s) | 391 |
| Tru1I | TTAA | 3 cut(s) | 192, 222, 486 |
| Tru9I | TTAA | 3 cut(s) | 192, 222, 486 |
| TscAI | CASTG | 1 cut(s) | 153 |
| TseFI | GTSAC | 2 cut(s) | 214, 428 |
| TseI | GCWGC | 1 cut(s) | 350 |
| Tsp45I | GTSAC | 2 cut(s) | 214, 428 |
| TspDTI | ATGAA | 6 cut(s) | 76, 398, 404, 457, 693, 720 |
| TspRI | CASTG | 1 cut(s) | 153 |
| VneI | GTGCAC | 1 cut(s) | 5 |
| VspI | ATTAAT | 2 cut(s) | 192, 222 |
| XceI | RCATGY | 1 cut(s) | 511 |
| XspI | CTAG | 3 cut(s) | 11, 18, 326 |