Rroxscaffold_4G00296320

Mediates both low-affinity uptake and efflux of sugar across the membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000596
Physical Location & Seq
Reverse (-)
16393981 .. 16395313
1333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00296320.1

Sequence Viewer

Length: 762 bp
ATGAAGCTGCAGTATCTGAGCAGCAAACAGATGGTGCACACAGATGCTAGGTTCGTGGTCGGTGTGGTTGGGAATGTCATCTCTGGCGGCCTTTTCCTCTCCCCAATTCCTACGTTCATACAAATATGGAGAAAAAAAGATGTGGAAGCTTTCGATCCAAGACCTTACTTTACAACGAGTGTTAAACTGTTTGTTCGGTGTTACTATGGATTACCATTCATTAACCCAAATAGCATTTTAGTTGTCACTATTAATGGAATTGGGCTATTTATAGAGCTTATATATCTTATCATATTCTTCTATTATGCCGCAACAAAAGGACGAAAGAGGGTTGCTGCATACTTTATATGTGAACTTATTTTATTTGGTGCTTTGGTGGTTGCAACTATGTTGGCAATACCTGAGCATAAAATGACGATGAATCGACCTTTGAGGGCTGTCGTAGTTGGTGTGATATGTGATTTTTTCAATGTCCTCATGTATGGCTCTACTTTGTTCAACGTGAGAGATGTCATTAAAACTAGGAGTGTCAAATATATGCCATTCACTCTCCTAATGGCTAACTTCCTGAATGGTTGTTGCTGGACATCCTATGCTCTTATTGGAAAAGTGGACTACTTCATTTTGATTAGCAACGGTCTCGGTGCAGTTTTTGGAGCAATTCAATTGATAATTTATGCAATATTCTACAAAACTACACCAAAAGATAAAGACCTCTCTAGCAAGACTACTAATGAAGTGCAACTCTGTACTAATGTCTAA

Protein Analysis

253

Amino Acids

28.57

Weight (kDa)

9.46

Isoelectric Point (pI)

32.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MtN3_slv PF03083 19 - 106 8.2e-17 Sugar efflux transporter for intercellular exchange
MtN3_slv PF03083 148 - 231 2.3e-20 Sugar efflux transporter for intercellular exchange
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000596)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40260 AT5G40260
fragaria_vesca FvH4_4g13312 FvH4_6g50390 FvH4_6g50390 FvH4_6g50580 FvH4_7g10730
malus_domestica MD17G1035200.v1.1
prunus_persica Prupe.3G283400_v2.0.a1
pyrus_communis pycom17g03250
rosa_chinensis RchiOBHm_Chr1g0349441 RchiOBHm_Chr1g0359391 RchiOBHm_Chr1g0359411 RchiOBHm_Chr1g0359541 RchiOBHm_Chr1g0359551 RchiOBHm_Chr1g0360181 RchiOBHm_Chr2g0131641 RchiOBHm_Chr2g0171121 RchiOBHm_Chr4g0413611 RchiOBHm_Chr5g0028121 RchiOBHm_Chr5g0037121 RchiOBHm_Chr5g0083341 RchiOBHm_Chr7g0239371
rosa_laevigata RLG00000008206 RLG00000022004 RLG00000027823 RLG00000027873 RLG00000028548 RLG00000037029
rosa_multiflora Rmu_sc0001287.1_g000004 Rmu_sc0001444.1_g000014 Rmu_sc0002245.1_g000046 Rmu_sc0002495.1_g000008 Rmu_sc0025358.1_g000001 Rmu_ssc0000358.1_g000004 Rmu_ssc0000358.1_g000005 Rmu_ssc0000368.1_g000047
rosa_roxburghii Rroxscaffold_2G00080810 Rroxscaffold_4G00296320 Rroxscaffold_5G00357560
rosa_rugosa Rorug01G0203900 Rorug01G0270900 Rorug01G0271000 Rorug01G0272100 Rorug01G0275700 Rorug02G0553600 Rorug04G0121500 Rorug04G0121600 Rorug05G0104200 Rorug05G0104300 Rorug05G0484300
rosa_samantha Rh1AG221100 Rh1AG284300 Rh1AG284400 Rh1AG289400 Rh1CG206000 Rh1CG272400 Rh1DG216500 Rh1DG279300 Rh1DG279400 Rh1DG284500 Rh2AG627300 Rh2BG637200 Rh2DG648800 Rh2DG654400 Rh4CG192300 Rh4DG176500 Rh5AG533700 Rh5BG561400 Rh5DG570300 Rh7CG498900 Rh7DG467000
rosa_wichuraiana Rw1G018790 Rw1G025240 Rw1G025250 Rw1G025680 Rw2G028530 Rw2G051920 Rw4G015140 Rw5G049910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 87, 309
AclWI GGATC 1 cut(s) 149
AfaI GTAC 1 cut(s) 751
AgsI TTSAA 3 cut(s) 469, 499, 665
AluBI AGCT 3 cut(s) 7, 149, 277
AluI AGCT 3 cut(s) 7, 149, 277
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 644
Alw44I GTGCAC 1 cut(s) 35
AlwI GGATC 1 cut(s) 149
AlwNI CAGNNNCTG 1 cut(s) 16
AoxI GGCC 1 cut(s) 88
ApaLI GTGCAC 1 cut(s) 35
ApeKI GCWGC 3 cut(s) 7, 21, 335
AseI ATTAAT 1 cut(s) 252
BaeGI GKGCMC 1 cut(s) 39
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 2 cut(s) 33, 322
BccI CCATC 1 cut(s) 25
BcgI CGANNNNNNTGC 2 cut(s) 622, 656
BcoDI GTCTC 1 cut(s) 644
BfaI CTAG 3 cut(s) 48, 522, 720
BfmI CTRYAG 1 cut(s) 8
BisI GCNGC 5 cut(s) 8, 22, 88, 309, 336
BlsI GCNGC 5 cut(s) 9, 23, 89, 310, 337
BmsI GCATC 1 cut(s) 34
Bpu10I CCTNAGC 1 cut(s) 402
BsaI GGTCTC 1 cut(s) 644
BseGI GGATG 1 cut(s) 587
BseMII CTCAG 2 cut(s) 8, 393
BseSI GKGCMC 1 cut(s) 39
BseXI GCAGC 2 cut(s) 33, 322
BsgI GTGCAG 1 cut(s) 666
BshFI GGCC 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 39
BsmAI GTCTC 1 cut(s) 644
BsnI GGCC 1 cut(s) 90
Bso31I GGTCTC 1 cut(s) 644
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 1 cut(s) 154
BspACI CCGC 2 cut(s) 87, 309
BspANI GGCC 1 cut(s) 90
BspCNI CTCAG 2 cut(s) 9, 394
BspMAI CTGCAG 1 cut(s) 12
BspPI GGATC 1 cut(s) 149
BspTNI GGTCTC 1 cut(s) 644
BssMI GATC 1 cut(s) 154
Bst4CI ACNGT 2 cut(s) 189, 638
BstDEI CTNAG 2 cut(s) 17, 402
BstF5I GGATG 1 cut(s) 587
BstKTI GATC 1 cut(s) 157
BstMAI GTCTC 1 cut(s) 644
BstMBI GATC 1 cut(s) 154
BstSFI CTRYAG 1 cut(s) 8
BstSLI GKGCMC 1 cut(s) 39
BstV1I GCAGC 2 cut(s) 33, 322
BsuRI GGCC 1 cut(s) 90
BtsCI GGATG 1 cut(s) 587
CaiI CAGNNNCTG 1 cut(s) 16
Csp6I GTAC 1 cut(s) 750
CviAII CATG 1 cut(s) 478
CviJI RGCY 8 cut(s) 7, 90, 149, 265, 277, 437, 486, 560
CviKI_1 RGCY 8 cut(s) 7, 90, 149, 265, 277, 437, 486, 560
CviQI GTAC 1 cut(s) 750
DdeI CTNAG 2 cut(s) 17, 402
DpnI GATC 1 cut(s) 156
DpnII GATC 1 cut(s) 154
Eco31I GGTCTC 1 cut(s) 644
FaeI CATG 1 cut(s) 481
FatI CATG 1 cut(s) 477
Fnu4HI GCNGC 5 cut(s) 8, 22, 88, 309, 336
FokI GGATG 1 cut(s) 574
Fsp4HI GCNGC 5 cut(s) 8, 22, 88, 309, 336
FspBI CTAG 3 cut(s) 48, 522, 720
GluI GCNGC 5 cut(s) 8, 22, 88, 309, 336
HaeIII GGCC 1 cut(s) 90
Hin1II CATG 1 cut(s) 481
HindIII AAGCTT 1 cut(s) 147
HinfI GANTC 1 cut(s) 421
Hpy166II GTNNAC 3 cut(s) 37, 353, 613
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 1 cut(s) 568
Hpy8I GTNNAC 3 cut(s) 37, 353, 613
HpyCH4III ACNGT 2 cut(s) 189, 638
HpyCH4IV ACGT 2 cut(s) 113, 501
HpyCH4V TGCA 7 cut(s) 10, 37, 338, 383, 647, 680, 742
HpyF3I CTNAG 2 cut(s) 17, 402
HpySE526I ACGT 2 cut(s) 113, 501
Hsp92II CATG 1 cut(s) 481
Kzo9I GATC 1 cut(s) 154
LmnI GCTCC 1 cut(s) 656
LpnPI CCDG 4 cut(s) 69, 414, 568, 581
Lsp1109I GCAGC 2 cut(s) 33, 322
LweI GCATC 1 cut(s) 34
MaeI CTAG 3 cut(s) 48, 522, 720
MaeII ACGT 2 cut(s) 113, 501
MaeIII GTNAC 2 cut(s) 200, 244
MalI GATC 1 cut(s) 156
MboI GATC 1 cut(s) 154
MboII GAAGA 1 cut(s) 289
MfeI CAATTG 1 cut(s) 665
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 5 cut(s) 105, 258, 660, 665, 672
MnlI CCTC 5 cut(s) 107, 321, 426, 485, 725
MseI TTAA 4 cut(s) 183, 222, 252, 516
MslI CAYNNNNRTG 1 cut(s) 42
MunI CAATTG 1 cut(s) 665
NdeII GATC 1 cut(s) 154
NlaIII CATG 1 cut(s) 481
NmuCI GTSAC 1 cut(s) 244
PfeI GAWTC 1 cut(s) 421
PkrI GCNGC 5 cut(s) 9, 23, 89, 310, 337
PshBI ATTAAT 1 cut(s) 252
PstI CTGCAG 1 cut(s) 12
PstNI CAGNNNCTG 1 cut(s) 16
RsaI GTAC 1 cut(s) 751
RsaNI GTAC 1 cut(s) 750
RseI CAYNNNNRTG 1 cut(s) 42
SaqAI TTAA 4 cut(s) 183, 222, 252, 516
SatI GCNGC 5 cut(s) 8, 22, 88, 309, 336
Sau3AI GATC 1 cut(s) 154
SduI GDGCHC 1 cut(s) 39
SfaNI GCATC 1 cut(s) 34
SfcI CTRYAG 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 42
Sse9I AATT 5 cut(s) 105, 258, 660, 665, 672
SsiI CCGC 2 cut(s) 87, 309
SspI AATATT 1 cut(s) 684
SspMI CTAG 3 cut(s) 48, 522, 720
TaaI ACNGT 2 cut(s) 189, 638
TaiI ACGT 2 cut(s) 116, 504
TaqI TCGA 2 cut(s) 153, 424
TasI AATT 5 cut(s) 105, 258, 660, 665, 672
TatI WGTACW 1 cut(s) 749
TauI GCSGC 2 cut(s) 90, 311
TfiI GAWTC 1 cut(s) 421
Tru1I TTAA 4 cut(s) 183, 222, 252, 516
Tru9I TTAA 4 cut(s) 183, 222, 252, 516
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 3 cut(s) 7, 21, 335
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 6 cut(s) 17, 106, 208, 434, 610, 750
VneI GTGCAC 1 cut(s) 35
VspI ATTAAT 1 cut(s) 252
XspI CTAG 3 cut(s) 48, 522, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.