Mediates both low-affinity uptake and efflux of sugar across the membrane
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 44 |
| AclWI | GGATC | 1 cut(s) | 149 |
| AcsI | RAATTY | 1 cut(s) | 11 |
| AfaI | GTAC | 1 cut(s) | 700 |
| AgsI | TTSAA | 5 cut(s) | 10, 184, 433, 463, 629 |
| AluBI | AGCT | 3 cut(s) | 149, 277, 694 |
| AluI | AGCT | 3 cut(s) | 149, 277, 694 |
| Alw21I | GWGCWC | 2 cut(s) | 279, 625 |
| Alw26I | GTCTC | 2 cut(s) | 608, 668 |
| AlwI | GGATC | 1 cut(s) | 149 |
| AoxI | GGCC | 1 cut(s) | 88 |
| ApeKI | GCWGC | 1 cut(s) | 691 |
| ApoI | RAATTY | 1 cut(s) | 11 |
| AseI | ATTAAT | 1 cut(s) | 252 |
| AspLEI | GCGC | 2 cut(s) | 44, 46 |
| AsuHPI | GGTGA | 1 cut(s) | 391 |
| BanII | GRGCYC | 2 cut(s) | 279, 373 |
| Bbv12I | GWGCWC | 2 cut(s) | 279, 625 |
| BbvI | GCAGC | 1 cut(s) | 703 |
| BccI | CCATC | 1 cut(s) | 370 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 586, 620 |
| BcoDI | GTCTC | 2 cut(s) | 608, 668 |
| BfmI | CTRYAG | 1 cut(s) | 652 |
| BisI | GCNGC | 1 cut(s) | 692 |
| BlsI | GCNGC | 1 cut(s) | 693 |
| BsaI | GGTCTC | 1 cut(s) | 608 |
| BseGI | GGATG | 1 cut(s) | 551 |
| BseMII | CTCAG | 1 cut(s) | 458 |
| BsePI | GCGCGC | 1 cut(s) | 42 |
| BseXI | GCAGC | 1 cut(s) | 703 |
| BsgI | GTGCAG | 1 cut(s) | 710 |
| Bsh1236I | CGCG | 1 cut(s) | 44 |
| BshFI | GGCC | 1 cut(s) | 90 |
| BsiHKAI | GWGCWC | 2 cut(s) | 279, 625 |
| BsmAI | GTCTC | 2 cut(s) | 608, 668 |
| BsnI | GGCC | 1 cut(s) | 90 |
| Bso31I | GGTCTC | 1 cut(s) | 608 |
| Bsp1286I | GDGCHC | 3 cut(s) | 279, 373, 625 |
| Bsp143I | GATC | 3 cut(s) | 78, 154, 417 |
| BspANI | GGCC | 1 cut(s) | 90 |
| BspCNI | CTCAG | 1 cut(s) | 459 |
| BspFNI | CGCG | 1 cut(s) | 44 |
| BspPI | GGATC | 1 cut(s) | 149 |
| BspTNI | GGTCTC | 1 cut(s) | 608 |
| BssHII | GCGCGC | 1 cut(s) | 42 |
| BssMI | GATC | 3 cut(s) | 78, 154, 417 |
| Bst4CI | ACNGT | 3 cut(s) | 178, 188, 602 |
| BstC8I | GCNNGC | 1 cut(s) | 44 |
| BstDEI | CTNAG | 3 cut(s) | 467, 486, 517 |
| BstF5I | GGATG | 1 cut(s) | 551 |
| BstFNI | CGCG | 1 cut(s) | 44 |
| BstHHI | GCGC | 2 cut(s) | 44, 46 |
| BstKTI | GATC | 3 cut(s) | 81, 157, 420 |
| BstMAI | GTCTC | 2 cut(s) | 608, 668 |
| BstMBI | GATC | 3 cut(s) | 78, 154, 417 |
| BstSFI | CTRYAG | 1 cut(s) | 652 |
| BstUI | CGCG | 1 cut(s) | 44 |
| BstV1I | GCAGC | 1 cut(s) | 703 |
| BsuRI | GGCC | 1 cut(s) | 90 |
| BtsCI | GGATG | 1 cut(s) | 551 |
| BtsIMutI | CAGTG | 1 cut(s) | 183 |
| Cac8I | GCNNGC | 1 cut(s) | 44 |
| CfoI | GCGC | 2 cut(s) | 44, 46 |
| Csp6I | GTAC | 1 cut(s) | 699 |
| CviJI | RGCY | 8 cut(s) | 90, 149, 265, 277, 371, 401, 450, 694 |
| CviKI_1 | RGCY | 8 cut(s) | 90, 149, 265, 277, 371, 401, 450, 694 |
| CviQI | GTAC | 1 cut(s) | 699 |
| DdeI | CTNAG | 3 cut(s) | 467, 486, 517 |
| DpnI | GATC | 3 cut(s) | 80, 156, 419 |
| DpnII | GATC | 3 cut(s) | 78, 154, 417 |
| Ecl136II | GAGCTC | 1 cut(s) | 277 |
| Eco24I | GRGCYC | 2 cut(s) | 279, 373 |
| Eco31I | GGTCTC | 1 cut(s) | 608 |
| Eco53kI | GAGCTC | 1 cut(s) | 277 |
| EcoICRI | GAGCTC | 1 cut(s) | 277 |
| EcoT38I | GRGCYC | 2 cut(s) | 279, 373 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 678, 710 |
| Fnu4HI | GCNGC | 1 cut(s) | 692 |
| FokI | GGATG | 1 cut(s) | 538 |
| FriOI | GRGCYC | 2 cut(s) | 279, 373 |
| Fsp4HI | GCNGC | 1 cut(s) | 692 |
| GlaI | GCGC | 2 cut(s) | 43, 45 |
| GluI | GCNGC | 1 cut(s) | 692 |
| HaeIII | GGCC | 1 cut(s) | 90 |
| HhaI | GCGC | 2 cut(s) | 44, 46 |
| Hin6I | GCGC | 2 cut(s) | 42, 44 |
| HinP1I | GCGC | 2 cut(s) | 42, 44 |
| HindIII | AAGCTT | 1 cut(s) | 147 |
| HinfI | GANTC | 1 cut(s) | 385 |
| HphI | GGTGA | 1 cut(s) | 391 |
| Hpy166II | GTNNAC | 1 cut(s) | 577 |
| Hpy188I | TCNGA | 1 cut(s) | 375 |
| Hpy188III | TCNNGA | 1 cut(s) | 532 |
| Hpy8I | GTNNAC | 1 cut(s) | 577 |
| HpyCH4III | ACNGT | 3 cut(s) | 178, 188, 602 |
| HpyCH4IV | ACGT | 1 cut(s) | 75 |
| HpyCH4V | TGCA | 3 cut(s) | 611, 644, 691 |
| HpyF3I | CTNAG | 3 cut(s) | 467, 486, 517 |
| HpySE526I | ACGT | 1 cut(s) | 75 |
| HspAI | GCGC | 2 cut(s) | 42, 44 |
| Kzo9I | GATC | 3 cut(s) | 78, 154, 417 |
| LmnI | GCTCC | 1 cut(s) | 620 |
| LpnPI | CCDG | 6 cut(s) | 32, 69, 181, 479, 532, 545 |
| Lsp1109I | GCAGC | 1 cut(s) | 703 |
| MaeII | ACGT | 1 cut(s) | 75 |
| MaeIII | GTNAC | 3 cut(s) | 200, 244, 334 |
| MalI | GATC | 3 cut(s) | 80, 156, 419 |
| MboI | GATC | 3 cut(s) | 78, 154, 417 |
| MboII | GAAGA | 1 cut(s) | 289 |
| MfeI | CAATTG | 1 cut(s) | 629 |
| MhlI | GDGCHC | 3 cut(s) | 279, 373, 625 |
| MluCI | AATT | 5 cut(s) | 11, 105, 258, 612, 629 |
| MmeI | TCCRAC | 1 cut(s) | 49 |
| MnlI | CCTC | 3 cut(s) | 107, 321, 390 |
| MseI | TTAA | 3 cut(s) | 222, 252, 480 |
| MunI | CAATTG | 1 cut(s) | 629 |
| MvnI | CGCG | 1 cut(s) | 44 |
| NdeII | GATC | 3 cut(s) | 78, 154, 417 |
| NmuCI | GTSAC | 1 cut(s) | 244 |
| PauI | GCGCGC | 1 cut(s) | 42 |
| PfeI | GAWTC | 1 cut(s) | 385 |
| PkrI | GCNGC | 1 cut(s) | 693 |
| PshBI | ATTAAT | 1 cut(s) | 252 |
| Psp124BI | GAGCTC | 1 cut(s) | 279 |
| PteI | GCGCGC | 1 cut(s) | 42 |
| RsaI | GTAC | 1 cut(s) | 700 |
| RsaNI | GTAC | 1 cut(s) | 699 |
| SacI | GAGCTC | 1 cut(s) | 279 |
| SaqAI | TTAA | 3 cut(s) | 222, 252, 480 |
| SatI | GCNGC | 1 cut(s) | 692 |
| Sau3AI | GATC | 3 cut(s) | 78, 154, 417 |
| SduI | GDGCHC | 3 cut(s) | 279, 373, 625 |
| SetI | ASST | 7 cut(s) | 78, 151, 166, 171, 279, 468, 696 |
| SfcI | CTRYAG | 1 cut(s) | 652 |
| Sse9I | AATT | 5 cut(s) | 11, 105, 258, 612, 629 |
| SstI | GAGCTC | 1 cut(s) | 279 |
| TaaI | ACNGT | 3 cut(s) | 178, 188, 602 |
| TaiI | ACGT | 1 cut(s) | 78 |
| TaqI | TCGA | 2 cut(s) | 153, 388 |
| TasI | AATT | 5 cut(s) | 11, 105, 258, 612, 629 |
| TatI | WGTACW | 1 cut(s) | 698 |
| TfiI | GAWTC | 1 cut(s) | 385 |
| Tru1I | TTAA | 3 cut(s) | 222, 252, 480 |
| Tru9I | TTAA | 3 cut(s) | 222, 252, 480 |
| TscAI | CASTG | 1 cut(s) | 183 |
| TseFI | GTSAC | 1 cut(s) | 244 |
| TseI | GCWGC | 1 cut(s) | 691 |
| Tsp45I | GTSAC | 1 cut(s) | 244 |
| TspDTI | ATGAA | 5 cut(s) | 17, 106, 398, 574, 699 |
| TspGWI | ACGGA | 1 cut(s) | 222 |
| TspRI | CASTG | 1 cut(s) | 183 |
| VspI | ATTAAT | 1 cut(s) | 252 |
| XapI | RAATTY | 1 cut(s) | 11 |