Rorug04G0121500

Mediates both low-affinity uptake and efflux of sugar across the membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000596
Physical Location & Seq
Forward (+)
19252158 .. 19256221
4064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0121500.1

Sequence Viewer

Length: 3363 bp
ATGTTCATGGAAAAGCTACAAACTTTCCTTTTTGGAGTAGCGCTACTACACTACTCATGCTTCCTTAAGTTGGCCATATCTTCACCTTCCAACTTCACTGATCAGTCTGCTTTGCTTGCCATTCAATCTCAAATCACTTTTGACCCCACCAATACTGTCTTGGGTGGTGGTAGCTGGATCACCAAAACAAGCTTCTGTAATTGGTTTGGGGTCTCTTGCAGTAAACGCAGGCAAAGAGTCACAGCCTTGGACCTTTCCTATATGAATCTCCAAGGCACCATTTCCCCTCATGTTGGCAATCTCTCCTTCCTAATCTCACTTGATCTCCGCAATAACAGCTTCTTGGGGTTCATACCACATGAAATCAGCCGTTTGCATCGCTTGAGGATACTTGTACTTCAAGGTAACAAGTTGGAAGGAAACATCCCTCCAACTTTACAGCTTTGCCACAAGCTCGAGTCCATATCCCTTTCAATAAACAGATTAAGTGGTCTCATACCTAAAGAGTTGGGTTTCCTTCCCAGGGTTCGTAAATTATATCTCGGTGCAAACAATCTCACAGGTGAAATTCCTTCGTCTCTGGGTAACATTTCAACCTTACAAGAATTAAGTCTGGACGAAAATGGTCTCACAGGTTCATTCCCTTCTGCTCTACTCAACGTATCTACTCTAGCTCTTATTTCCCTATCTGGCAATTACATGTCAGGTAGTCTTCCTGTAGATCTTTGCCGTAATTGGCCTAATACTTATTACATTTCTCTATCTGGCAACAAATTCGGTGGCCAACTGACTTTCGGAATAAGCCAATGTAGAGAGCTTGAGATCTTATCTCTGTCATACAATAATTTTGTCGGAAGTATTCCGGGAGAAATTGGCAGTTTACAACATCTTAAAACTCTATTTCTTGGTGTCAATAACCTAACTGGTGCTATACCTCCAACAATAGGTAACCTGTCAAACTTGCAGTTCTTTACTGTTCCAGATAATAATTTAGAGGGAAGTATTCCAAGTGATTTGGGGCATCTTTCTAAACTAAATTATCTCTCTTTTGCTCGGAATAGCCTAACAGGTGAAATACCTGAGTCCATTTTCAATATCTCCTCCTTAAATACTTTACAAGTACAAACTAATGCTCTTTCTGGAGAATTTCCTTCTCGAGCTATGATTTCTAACCTCAATACCCTATACTGTTATACCAATCACATTACAGGAAATCTACCATCCTATTTTTCAAATTTAAGCCAGCTTGTTGAGCTTGATGTTTCCAACAACTCACTTTATGGACCAATACCCATGAACCTTGGTAGATCAAAATACCTCGAATTCCTTAACTTGGGAGGAAATCAGTTGTCAGGGGAACCTGGAGTTCTTGAACTTAGGTTCCTTTCTTCTATATTTAATAGCAGTTCACTGAAACATTTATTTTTGAGTAAAAATCCTCTAGACGGGATCATGCCCGATTCATTTGGAAATTTCTCATATTCCCTCGAGGTATTTTTTGCAACTGCCTGCCAGATTAAGGGCCCTATACCCAAGACGATTCAGTTGTTGAAAAATCTGACCTTCCTTGCCTTAAGTGACAACAATATTAGTGGGAAAATACCATCATCAATTGGAGCATTGGAAATGTTGCAGAGATTGTATCTTGAAAACAACAAGATTGAAGGGCTCATTCCTAATGAGCTTTGTCTTTTAAGGAACCTGGGAGATCTATCACTAGGAAATAATCAAATCTCTGGATCAATCCCAAATTGCATTGCAAATCTCAGTCGCATTCAAAGACTTCTTCTAAGTTCCAATAGATTGACATCTGCAGTACCAGTGAATTTATGGAATCTTGAAAATCTTCTGTTCTTAAATTTGTCATCCAATATTTTCAGTGGATACTTACCTCCAAGCATGAAAAGGTCGGTTGCTGTTCAGATGGTCGACTTATCTCACAATCAAGTTACTGGGAACATTTCAAGCATTGTCGGAGCCTTCCCAAGCATAAACTCTCTGAGCTTAGCAAACAACTTGTTTATAGGATCTCTTCCGCAAACTTTGGGTGATCTGAAAGGATTGGAGTTTTTGGACCTATCAAACAATAATCTGTCTGGGCCAATTCCAAAGTCTCTTGAAACACTGAAATATCTGAACTACTTAAATTTCTCTTTTAATCAACTCTCTGGAGAGATTCCTTCTAGTGGAGCTTTTATCAACTTCACTGCCCTATCATTTTTGGAGAATAGAGGCCTTTGTGGGAGACCGGAGTTTGGAGTCCCACCTTGCATGGCTCTTGGAACTCAGAAGTCAAAGATGGCAAATCATTTACTCAAGTATGTTCTTCCAGGCATTGCATCAACAATGATCTTGATAGCTCTCATATACATGGTGACAAAGTGTCGAAAAAGAGATGAAGGCATTCCAAGTTCAGTACAAGGGTTAACAACAACAGAGCATAGAATGATATCATATCATCAACTCCGTCGTGCTACAAATGGCTTCTGTGAAAGCAACTTGCTTGGAGTTGGAAGTTTTGGCTCGGTATACAAGGGGATTTTAACAGATGGGACAATTGTTGCTGTTAAAGTTCTAAATTTGCAAATGGAGGGAGCCTTTAAAAGTTTTGACGCAGAATGTAAAGTTTGGCGGAGAATTCGGCACAGAAATCTTGTCAAGATCATGACTACATGCTCTAACCATGAGGTAAGAGCTTTGGTGCTGCAATACATGTCTAATGGCAGCCTTGAGAAGTGGCTATACTCTCACAACTATTGTTTGACTCTTCTGCAAAGAGTGAGCATGTTGATCGACATTGCACTGGCTTTGGACTATCTCCATCATGGTCAATCAGAAGGTGTGGTGCACTGTGATTTGAAGCCTAGCAATATCCTTCTCGATGAAGACATGGTTGCACATGTTGGAGACTTCGGGCTTGCAAAAATTATAGCAATTAACAAAGATGCAACACAGACTAGAACTCTCGGCACACTCGGCTACGTTGCACCAGAGTACGGTTCTGCCGGAGTAGTGTCCGCAAGAGGTGATATTTATAGTTTTGGGATAATGTTGATGGAAATAATTGTGAGAAAGAAACCCACAGATGAAATGTTTGCTGGAGAACTGACTTTGAGACAATGGATAAATGCTTCGCTTCCTGACAGTGTTCTCGAAGTAGTGGATGCTGGCCTATTGAGCATAGAAGATGGAACTAGAGACATGAATGCCACAGTAAATACCATTTTGTCAATACTGGAAATAGGTTTGGGATGTTCTGAAGAAGTGGCTCAGGAAAGAATCAACATTAAGGATGTAGTGCCCAAGCTCACGAAAATAAAATGTGCACTAGAAGATAGAAATGGAGGTGTTTATTATCCATGA

Protein Analysis

1120

Amino Acids

122.77

Weight (kDa)

7.51

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 83 - 213 1.2e-07 Leucine-rich repeat region
LRR_8 PF13855 271 - 331 8.7e-06 Leucine rich repeat
LRR_14 PF23598 272 - 350 8.5e-07 Leucine-rich repeat region
LRR_14 PF23598 337 - 444 3.9e-07 Leucine-rich repeat region
LRR_14 PF23598 510 - 622 4.1e-09 Leucine-rich repeat region
Pkinase PF00069 832 - 1029 1.7e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 833 - 1031 4.1e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000596)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40260 AT5G40260
fragaria_vesca FvH4_4g13312 FvH4_6g50390 FvH4_6g50390 FvH4_6g50580 FvH4_7g10730
malus_domestica MD17G1035200.v1.1
prunus_persica Prupe.3G283400_v2.0.a1
pyrus_communis pycom17g03250
rosa_chinensis RchiOBHm_Chr1g0349441 RchiOBHm_Chr1g0359391 RchiOBHm_Chr1g0359411 RchiOBHm_Chr1g0359541 RchiOBHm_Chr1g0359551 RchiOBHm_Chr1g0360181 RchiOBHm_Chr2g0131641 RchiOBHm_Chr2g0171121 RchiOBHm_Chr4g0413611 RchiOBHm_Chr5g0028121 RchiOBHm_Chr5g0037121 RchiOBHm_Chr5g0083341 RchiOBHm_Chr7g0239371
rosa_laevigata RLG00000008206 RLG00000022004 RLG00000027823 RLG00000027873 RLG00000028548 RLG00000037029
rosa_multiflora Rmu_sc0001287.1_g000004 Rmu_sc0001444.1_g000014 Rmu_sc0002245.1_g000046 Rmu_sc0002495.1_g000008 Rmu_sc0025358.1_g000001 Rmu_ssc0000358.1_g000004 Rmu_ssc0000358.1_g000005 Rmu_ssc0000368.1_g000047
rosa_roxburghii Rroxscaffold_2G00080810 Rroxscaffold_4G00296320 Rroxscaffold_5G00357560
rosa_rugosa Rorug01G0203900 Rorug01G0270900 Rorug01G0271000 Rorug01G0272100 Rorug01G0275700 Rorug02G0553600 Rorug04G0121500 Rorug04G0121600 Rorug05G0104200 Rorug05G0104300 Rorug05G0484300
rosa_samantha Rh1AG221100 Rh1AG284300 Rh1AG284400 Rh1AG289400 Rh1CG206000 Rh1CG272400 Rh1DG216500 Rh1DG279300 Rh1DG279400 Rh1DG284500 Rh2AG627300 Rh2BG637200 Rh2DG648800 Rh2DG654400 Rh4CG192300 Rh4DG176500 Rh5AG533700 Rh5BG561400 Rh5DG570300 Rh7CG498900 Rh7DG467000
rosa_wichuraiana Rw1G018790 Rw1G025240 Rw1G025250 Rw1G025680 Rw2G028530 Rw2G051920 Rw4G015140 Rw5G049910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1487
AccB1I GGYRCC 1 cut(s) 275
AccI GTMKAC 2 cut(s) 1929, 2529
AciI CCGC 4 cut(s) 328, 2036, 2632, 3018
AclWI GGATC 4 cut(s) 185, 1457, 1747, 2035
AcoI YGGCCR 2 cut(s) 72, 781
AcuI CTGAAG 1 cut(s) 3279
AfaI GTAC 5 cut(s) 396, 1122, 1818, 2418, 2996
AfeI AGCGCT 1 cut(s) 42
AflII CTTAAG 2 cut(s) 65, 1573
AflIII ACRYGT 3 cut(s) 699, 2712, 2899
AhdI GACNNNNNGTC 1 cut(s) 2382
AjnI CCWGG 4 cut(s) 521, 1360, 1701, 2329
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AloI GAACNNNNNNTCC 2 cut(s) 1365, 1397
Alw21I GWGCWC 2 cut(s) 2850, 3328
Alw26I GTCTC 9 cut(s) 217, 497, 582, 632, 2118, 2239, 2901, 3109, 3192
Alw44I GTGCAC 2 cut(s) 2846, 3324
AlwI GGATC 4 cut(s) 185, 1457, 1747, 2035
Ama87I CYCGRG 3 cut(s) 455, 1155, 1487
Aor51HI AGCGCT 1 cut(s) 42
AoxI GGCC 7 cut(s) 72, 737, 781, 1522, 2099, 2233, 3169
ApaI GGGCCC 1 cut(s) 1526
ApaLI GTGCAC 2 cut(s) 2846, 3324
ApeKI GCWGC 2 cut(s) 2704, 2724
Asp700I GAANNNNTTC 2 cut(s) 1845, 2403
AspLEI GCGC 1 cut(s) 43
AspS9I GGNCC 6 cut(s) 250, 1283, 1522, 1523, 2074, 2099
AsuC2I CCSGG 1 cut(s) 864
AsuHPI GGTGA 7 cut(s) 75, 172, 575, 1082, 2060, 2386, 3038
AvaI CYCGRG 3 cut(s) 455, 1155, 1487
AvaII GGWCC 3 cut(s) 250, 1283, 2074
BaeGI GKGCMC 4 cut(s) 1526, 2850, 3303, 3328
BalI TGGCCA 2 cut(s) 74, 783
BanI GGYRCC 1 cut(s) 275
BanII GRGCYC 2 cut(s) 1526, 1671
BarI GAAGNNNNNNTAC 2 cut(s) 2726, 2758
BbsI GAAGAC 2 cut(s) 704, 2892
Bbv12I GWGCWC 2 cut(s) 2850, 3328
BbvI GCAGC 2 cut(s) 2691, 2736
BccI CCATC 8 cut(s) 1228, 1612, 1918, 2293, 2543, 2829, 3049, 3182
BceAI ACGGC 2 cut(s) 354, 714
BcgI CGANNNNNNTGC 4 cut(s) 757, 791, 2773, 2807
BciT130I CCWGG 4 cut(s) 523, 1362, 1703, 2331
BciVI GTATCC 2 cut(s) 381, 1877
BclI TGATCA 1 cut(s) 100
BcnI CCSGG 1 cut(s) 864
BcoDI GTCTC 9 cut(s) 217, 497, 582, 632, 2118, 2239, 2901, 3109, 3192
BfaI CTAG 8 cut(s) 671, 1442, 1718, 2184, 2865, 2958, 3195, 3329
BfmI CTRYAG 2 cut(s) 717, 1812
BfoI RGCGCY 1 cut(s) 44
BfrI CTTAAG 2 cut(s) 65, 1573
BfuI GTATCC 2 cut(s) 381, 1877
BglII AGATCT 3 cut(s) 721, 822, 1708
BisI GCNGC 2 cut(s) 2705, 2725
BlpI GCTNAGC 1 cut(s) 2005
BlsI GCNGC 2 cut(s) 2706, 2726
Bme1390I CCNGG 5 cut(s) 523, 864, 1362, 1703, 2331
Bme18I GGWCC 3 cut(s) 250, 1283, 2074
BmeRI GACNNNNNGTC 1 cut(s) 2382
BmeT110I CYCGRG 3 cut(s) 455, 1155, 1487
BmgT120I GGNCC 6 cut(s) 250, 1283, 1522, 1523, 2074, 2099
BmiI GGNNCC 7 cut(s) 277, 1359, 1382, 1524, 1700, 1978, 2596
BmrFI CCNGG 5 cut(s) 523, 864, 1362, 1703, 2331
BmrI ACTGGG 1 cut(s) 1962
BmsI GCATC 5 cut(s) 385, 1030, 2348, 2935, 3154
BmuI ACTGGG 1 cut(s) 1962
BpiI GAAGAC 2 cut(s) 704, 2892
BpmI CTGGAG 4 cut(s) 1161, 1383, 2190, 3120
Bpu10I CCTNAGC 1 cut(s) 3270
Bpu1102I GCTNAGC 1 cut(s) 2005
BpuEI CTTGAG 4 cut(s) 403, 839, 2300, 2750
BpuMI CCSGG 1 cut(s) 864
BsaBI GATNNNNATC 1 cut(s) 1807
BsaI GGTCTC 4 cut(s) 217, 497, 632, 2239
BsaJI CCNNGG 6 cut(s) 246, 271, 521, 522, 1300, 1702
BsaWI WCCGGW 1 cut(s) 2248
BsaXI ACNNNNNCTCC 2 cut(s) 309, 339
Bse1I ACTGG 5 cut(s) 928, 1820, 1957, 2808, 3240
Bse3DI GCAATG 3 cut(s) 1755, 2334, 2796
Bse8I GATNNNNATC 1 cut(s) 1807
BseBI CCWGG 4 cut(s) 523, 1362, 1703, 2331
BseDI CCNNGG 6 cut(s) 246, 271, 521, 522, 1300, 1702
BseGI GGATG 6 cut(s) 423, 1220, 1865, 3169, 3257, 3298
BseJI GATNNNNATC 1 cut(s) 1807
BseMI GCAATG 3 cut(s) 1755, 2334, 2796
BseMII CTCAG 5 cut(s) 1071, 1780, 1991, 2300, 3284
BseNI ACTGG 5 cut(s) 928, 1820, 1957, 2808, 3240
BseRI GAGGAG 1 cut(s) 1090
BseSI GKGCMC 4 cut(s) 1526, 2850, 3303, 3328
BseXI GCAGC 2 cut(s) 2691, 2736
BshFI GGCC 7 cut(s) 74, 739, 783, 1524, 2101, 2235, 3171
BshNI GGYRCC 1 cut(s) 275
BsiHKAI GWGCWC 2 cut(s) 2850, 3328
BsiHKCI CYCGRG 3 cut(s) 455, 1155, 1487
BsiSI CCGG 3 cut(s) 863, 2249, 3006
BslFI GGGAC 2 cut(s) 2246, 2566
BsmAI GTCTC 9 cut(s) 217, 497, 582, 632, 2118, 2239, 2901, 3109, 3192
BsmBI CGTCTC 1 cut(s) 582
BsmFI GGGAC 2 cut(s) 2246, 2566
BsmI GAATGC 3 cut(s) 1773, 2403, 3211
BsnI GGCC 7 cut(s) 74, 739, 783, 1524, 2101, 2235, 3171
Bso31I GGTCTC 4 cut(s) 217, 497, 632, 2239
BsoBI CYCGRG 3 cut(s) 455, 1155, 1487
Bsp120I GGGCCC 1 cut(s) 1522
Bsp1286I GDGCHC 5 cut(s) 1526, 1671, 2850, 3303, 3328
Bsp1720I GCTNAGC 1 cut(s) 2005
BspACI CCGC 4 cut(s) 328, 2036, 2632, 3018
BspANI GGCC 7 cut(s) 74, 739, 783, 1524, 2101, 2235, 3171
BspCNI CTCAG 5 cut(s) 1072, 1779, 1992, 2299, 3283
BspHI TCATGA 1 cut(s) 2664
BspLI GGNNCC 7 cut(s) 277, 1359, 1382, 1524, 1700, 1978, 2596
BspMAI CTGCAG 1 cut(s) 1816
BspPI GGATC 4 cut(s) 185, 1457, 1747, 2035
BspT107I GGYRCC 1 cut(s) 275
BspTI CTTAAG 2 cut(s) 65, 1573
BspTNI GGTCTC 4 cut(s) 217, 497, 632, 2239
BsrDI GCAATG 3 cut(s) 1755, 2334, 2796
BsrI ACTGG 5 cut(s) 928, 1820, 1957, 2808, 3240
BssECI CCNNGG 6 cut(s) 246, 271, 521, 522, 1300, 1702
BssNAI GTATAC 1 cut(s) 2530
BssT1I CCWWGG 3 cut(s) 246, 271, 1300
Bst1107I GTATAC 1 cut(s) 2530
Bst2UI CCWGG 4 cut(s) 523, 1362, 1703, 2331
Bst4CI ACNGT 7 cut(s) 157, 976, 1190, 2852, 2999, 3146, 3214
Bst6I CTCTTC 2 cut(s) 2037, 2772
BstAFI CTTAAG 2 cut(s) 65, 1573
BstC8I GCNNGC 6 cut(s) 117, 230, 1244, 1510, 2919, 3169
BstDEI CTNAG 8 cut(s) 1080, 1376, 1766, 1790, 2000, 2005, 2286, 3270
BstEII GGTNACC 1 cut(s) 947
BstF5I GGATG 6 cut(s) 423, 1220, 1865, 3169, 3257, 3298
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 1 cut(s) 43
BstMAI GTCTC 9 cut(s) 217, 497, 582, 632, 2118, 2239, 2901, 3109, 3192
BstMWI GCNNNNNNNGC 6 cut(s) 116, 225, 336, 1252, 2976, 3177
BstNI CCWGG 4 cut(s) 523, 1362, 1703, 2331
BstNSI RCATGY 5 cut(s) 703, 2676, 2716, 2788, 2903
BstPI GGTNACC 1 cut(s) 947
BstSCI CCNGG 5 cut(s) 521, 862, 1360, 1701, 2329
BstSFI CTRYAG 2 cut(s) 717, 1812
BstSLI GKGCMC 4 cut(s) 1526, 2850, 3303, 3328
BstV1I GCAGC 2 cut(s) 2691, 2736
BstV2I GAAGAC 2 cut(s) 704, 2892
BstX2I RGATCY 4 cut(s) 721, 822, 1708, 2027
BstYI RGATCY 4 cut(s) 721, 822, 1708, 2027
BstZ17I GTATAC 1 cut(s) 2530
BsuI GTATCC 2 cut(s) 381, 1877
BsuRI GGCC 7 cut(s) 74, 739, 783, 1524, 2101, 2235, 3171
BtgZI GCGATG 1 cut(s) 362
BtsCI GGATG 6 cut(s) 423, 1220, 1865, 3169, 3257, 3298
BtsI GCAGTG 1 cut(s) 2205
BtsIMutI CAGTG 9 cut(s) 96, 1409, 1827, 1885, 2123, 2205, 2801, 2848, 3151
Cac8I GCNNGC 6 cut(s) 117, 230, 1244, 1510, 2919, 3169
CciI TCATGA 1 cut(s) 2664
CfoI GCGC 1 cut(s) 43
Cfr13I GGNCC 6 cut(s) 250, 1283, 1522, 1523, 2074, 2099
CseI GACGC 1 cut(s) 2621
Csp6I GTAC 5 cut(s) 395, 1121, 1817, 2417, 2995
CspCI CAANNNNNGTGG 4 cut(s) 760, 795, 1573, 1608
CviQI GTAC 5 cut(s) 395, 1121, 1817, 2417, 2995
DdeI CTNAG 8 cut(s) 1080, 1376, 1766, 1790, 2000, 2005, 2286, 3270
DraI TTTAAA 1 cut(s) 2602
DriI GACNNNNNGTC 1 cut(s) 2382
EaeI YGGCCR 2 cut(s) 72, 781
Eam1104I CTCTTC 2 cut(s) 2037, 2772
Eam1105I GACNNNNNGTC 1 cut(s) 2382
EarI CTCTTC 2 cut(s) 2037, 2772
EciI GGCGGA 1 cut(s) 2647
Eco130I CCWWGG 3 cut(s) 246, 271, 1300
Eco147I AGGCCT 1 cut(s) 2235
Eco24I GRGCYC 2 cut(s) 1526, 1671
Eco31I GGTCTC 4 cut(s) 217, 497, 632, 2239
Eco32I GATATC 1 cut(s) 2451
Eco47I GGWCC 3 cut(s) 250, 1283, 2074
Eco47III AGCGCT 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 3279
Eco88I CYCGRG 3 cut(s) 455, 1155, 1487
Eco91I GGTNACC 1 cut(s) 947
EcoO109I RGGNCCY 2 cut(s) 1522, 1523
EcoO65I GGTNACC 1 cut(s) 947
EcoRI GAATTC 2 cut(s) 1322, 2637
EcoRII CCWGG 4 cut(s) 521, 1360, 1701, 2329
EcoRV GATATC 1 cut(s) 2451
EcoT14I CCWWGG 3 cut(s) 246, 271, 1300
EcoT38I GRGCYC 2 cut(s) 1526, 1671
ErhI CCWWGG 3 cut(s) 246, 271, 1300
Esp3I CGTCTC 1 cut(s) 582
FaqI GGGAC 2 cut(s) 2246, 2566
FbaI TGATCA 1 cut(s) 100
FblI GTMKAC 2 cut(s) 1929, 2529
Fnu4HI GCNGC 2 cut(s) 2705, 2725
FokI GGATG 6 cut(s) 410, 1207, 1852, 3176, 3264, 3305
FriOI GRGCYC 2 cut(s) 1526, 1671
Fsp4HI GCNGC 2 cut(s) 2705, 2725
FspBI CTAG 8 cut(s) 671, 1442, 1718, 2184, 2865, 2958, 3195, 3329
GlaI GCGC 1 cut(s) 42
GluI GCNGC 2 cut(s) 2705, 2725
GsuI CTGGAG 4 cut(s) 1161, 1383, 2190, 3120
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 7 cut(s) 74, 739, 783, 1524, 2101, 2235, 3171
HapII CCGG 3 cut(s) 863, 2249, 3006
HgaI GACGC 1 cut(s) 2621
HhaI GCGC 1 cut(s) 43
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HincII GTYRAC 2 cut(s) 1930, 2427
HindII GTYRAC 2 cut(s) 1930, 2427
HindIII AAGCTT 1 cut(s) 190
HpaI GTTAAC 1 cut(s) 2427
HpaII CCGG 3 cut(s) 863, 2249, 3006
HphI GGTGA 7 cut(s) 75, 172, 575, 1082, 2060, 2386, 3038
Hpy166II GTNNAC 8 cut(s) 224, 881, 1409, 1930, 2427, 2530, 2848, 3326
Hpy8I GTNNAC 8 cut(s) 224, 881, 1409, 1930, 2427, 2530, 2848, 3326
Hpy99I CGWCG 1 cut(s) 2472
HpyCH4III ACNGT 7 cut(s) 157, 976, 1190, 2852, 2999, 3146, 3214
HpyCH4IV ACGT 2 cut(s) 660, 2982
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 225, 336, 1252, 2976, 3177
HpyF3I CTNAG 8 cut(s) 1080, 1376, 1766, 1790, 2000, 2005, 2286, 3270
HpySE526I ACGT 2 cut(s) 660, 2982
HspAI GCGC 1 cut(s) 41
Ksp22I TGATCA 1 cut(s) 100
KspAI GTTAAC 1 cut(s) 2427
LmnI GCTCC 4 cut(s) 1616, 1976, 2189, 2594
Lsp1109I GCAGC 2 cut(s) 2691, 2736
LweI GCATC 5 cut(s) 385, 1030, 2348, 2935, 3154
MaeI CTAG 8 cut(s) 671, 1442, 1718, 2184, 2865, 2958, 3195, 3329
MaeII ACGT 2 cut(s) 660, 2982
MaeIII GTNAC 7 cut(s) 238, 404, 584, 947, 1577, 1948, 2374
MfeI CAATTG 2 cut(s) 1611, 2556
MflI RGATCY 4 cut(s) 721, 822, 1708, 2027
MhlI GDGCHC 5 cut(s) 1526, 1671, 2850, 3303, 3328
MlsI TGGCCA 2 cut(s) 74, 783
MluNI TGGCCA 2 cut(s) 74, 783
MlyI GAGTC 5 cut(s) 246, 467, 1091, 2268, 2758
MmeI TCCRAC 9 cut(s) 114, 393, 455, 832, 962, 1290, 1954, 2491, 2884
Mox20I TGGCCA 2 cut(s) 74, 783
MroXI GAANNNNTTC 2 cut(s) 1845, 2403
MscI TGGCCA 2 cut(s) 74, 783
MslI CAYNNNNRTG 1 cut(s) 2369
Msp20I TGGCCA 2 cut(s) 74, 783
MspCI CTTAAG 2 cut(s) 65, 1573
MspI CCGG 3 cut(s) 863, 2249, 3006
MspR9I CCNGG 5 cut(s) 523, 864, 1362, 1703, 2331
MunI CAATTG 2 cut(s) 1611, 2556
Mva1269I GAATGC 3 cut(s) 1773, 2403, 3211
MvaI CCWGG 4 cut(s) 523, 1362, 1703, 2331
MwoI GCNNNNNNNGC 6 cut(s) 116, 225, 336, 1252, 2976, 3177
NciI CCSGG 1 cut(s) 864
NlaIV GGNNCC 7 cut(s) 277, 1359, 1382, 1524, 1700, 1978, 2596
NmeAIII GCCGAG 2 cut(s) 2946, 2955
NmuCI GTSAC 3 cut(s) 238, 1577, 2374
NspI RCATGY 5 cut(s) 703, 2676, 2716, 2788, 2903
PaeR7I CTCGAG 3 cut(s) 455, 1155, 1487
PagI TCATGA 1 cut(s) 2664
PasI CCCWGGG 1 cut(s) 522
PceI AGGCCT 1 cut(s) 2235
PciI ACATGT 3 cut(s) 699, 2712, 2899
PctI GAATGC 3 cut(s) 1773, 2403, 3211
PdmI GAANNNNTTC 2 cut(s) 1845, 2403
PfeI GAWTC 6 cut(s) 265, 1460, 1540, 1834, 2176, 3279
PfoI TCCNGGA 1 cut(s) 862
PkrI GCNGC 2 cut(s) 2706, 2726
PleI GAGTC 5 cut(s) 245, 466, 1090, 2267, 2758
PpsI GAGTC 5 cut(s) 245, 466, 1090, 2267, 2758
PscI ACATGT 3 cut(s) 699, 2712, 2899
Psp6I CCWGG 4 cut(s) 521, 1360, 1701, 2329
PspEI GGTNACC 1 cut(s) 947
PspGI CCWGG 4 cut(s) 521, 1360, 1701, 2329
PspN4I GGNNCC 7 cut(s) 277, 1359, 1382, 1524, 1700, 1978, 2596
PspOMI GGGCCC 1 cut(s) 1522
PspPI GGNCC 6 cut(s) 250, 1283, 1522, 1523, 2074, 2099
PspXI VCTCGAGB 2 cut(s) 455, 1487
PstI CTGCAG 1 cut(s) 1816
PsuI RGATCY 4 cut(s) 721, 822, 1708, 2027
RsaI GTAC 5 cut(s) 396, 1122, 1818, 2418, 2996
RsaNI GTAC 5 cut(s) 395, 1121, 1817, 2417, 2995
RseI CAYNNNNRTG 1 cut(s) 2369
SalI GTCGAC 1 cut(s) 1928
SatI GCNGC 2 cut(s) 2705, 2725
Sau96I GGNCC 6 cut(s) 250, 1283, 1522, 1523, 2074, 2099
SchI GAGTC 5 cut(s) 246, 467, 1091, 2268, 2758
ScrFI CCNGG 5 cut(s) 523, 864, 1362, 1703, 2331
SduI GDGCHC 5 cut(s) 1526, 1671, 2850, 3303, 3328
SfaNI GCATC 5 cut(s) 385, 1030, 2348, 2935, 3154
SfcI CTRYAG 2 cut(s) 717, 1812
Sfr274I CTCGAG 3 cut(s) 455, 1155, 1487
SinI GGWCC 3 cut(s) 250, 1283, 2074
SlaI CTCGAG 3 cut(s) 455, 1155, 1487
SmiMI CAYNNNNRTG 1 cut(s) 2369
SmlI CTYRAG 9 cut(s) 65, 382, 455, 818, 1155, 1487, 1573, 2315, 2729
SmoI CTYRAG 9 cut(s) 65, 382, 455, 818, 1155, 1487, 1573, 2315, 2729
SseBI AGGCCT 1 cut(s) 2235
SsiI CCGC 4 cut(s) 328, 2036, 2632, 3018
SspI AATATT 2 cut(s) 1588, 1873
SspMI CTAG 8 cut(s) 671, 1442, 1718, 2184, 2865, 2958, 3195, 3329
StuI AGGCCT 1 cut(s) 2235
StyD4I CCNGG 5 cut(s) 521, 862, 1360, 1701, 2329
StyI CCWWGG 3 cut(s) 246, 271, 1300
TaaI ACNGT 7 cut(s) 157, 976, 1190, 2852, 2999, 3146, 3214
TaiI ACGT 2 cut(s) 663, 2985
TaqI TCGA 9 cut(s) 456, 1156, 1320, 1488, 1929, 2386, 2793, 2880, 3153
TatI WGTACW 3 cut(s) 394, 1120, 2416
TfiI GAWTC 6 cut(s) 265, 1460, 1540, 1834, 2176, 3279
TscAI CASTG 9 cut(s) 103, 1416, 1827, 1885, 2130, 2212, 2808, 2855, 3151
TseFI GTSAC 3 cut(s) 238, 1577, 2374
TseI GCWGC 2 cut(s) 2704, 2724
Tsp45I GTSAC 3 cut(s) 238, 1577, 2374
TspGWI ACGGA 1 cut(s) 2456
TspRI CASTG 9 cut(s) 103, 1416, 1827, 1885, 2130, 2212, 2808, 2855, 3151
Vha464I CTTAAG 2 cut(s) 65, 1573
VneI GTGCAC 2 cut(s) 2846, 3324
VpaK11BI GGWCC 3 cut(s) 250, 1283, 2074
XbaI TCTAGA 1 cut(s) 1441
XceI RCATGY 5 cut(s) 703, 2676, 2716, 2788, 2903
XcmI CCANNNNNNNNNTGG 2 cut(s) 157, 1827
XhoI CTCGAG 3 cut(s) 455, 1155, 1487
XmiI GTMKAC 2 cut(s) 1929, 2529
XmnI GAANNNNTTC 2 cut(s) 1845, 2403
XspI CTAG 8 cut(s) 671, 1442, 1718, 2184, 2865, 2958, 3195, 3329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.