Rmu_sc0000367.1_g000140

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000367.1
OG0001195
Physical Location & Seq
Reverse (-)
683942 .. 685332
1391 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 717 bp
atggctggacccgacgcaagctgtgtggatgagatctgccatctccctatgaaccctagggagatgaaactgtttcaaaaggcttcagttccagggaccgggcacctagttctaaaaccagcaatcgaaaaggttgcggaggatccactggttcagaactgcaaacatgcaggcacctatacgtatactcctgtcgacttcagttccaagttcagaggaccagtggctggtggaggcccttctatgtacaaagaagtgccttgcaatgagtgttctaaggagcaccctccttattcttatcgcccttacgcgccttgtcatatttgttataaggttcaccaccatagttattgcccttacttagaccatgtcccacagggtgcaacttttcaccatggctatgaaataatttgtggatgtggtaatgaatttaatgaggataagtgggtttgtacctcttgtggtgggaacagagcagtgcttaaggccaagcattgttccatttgtaatagcgtgaaacagcacggcacatatgaatgcccgaaggacaagattcttgctgccaagtataaattggccagagacaagaggcgttcagttgtgccgaataggattccctatgaaactgtttgtattccaaaatcatcatcagaaaagatctatgttccaactcctcctcctgggtgggtttgtggacagatgaaagaaggctcgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

26.42

Weight (kDa)

8.59

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 330
AccB1I GGYRCC 2 cut(s) 102, 173
AccB7I CCANNNNNTGG 1 cut(s) 227
AccI GTMKAC 2 cut(s) 185, 195
AccII CGCG 1 cut(s) 311
AciI CCGC 1 cut(s) 137
AclWI GGATC 2 cut(s) 137, 150
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 1 cut(s) 428
AcuI CTGAAG 2 cut(s) 69, 184
AdeI CACNNNGTG 1 cut(s) 380
AfaI GTAC 2 cut(s) 248, 454
AfiI CCNNNNNNNGG 3 cut(s) 98, 227, 680
AflII CTTAAG 1 cut(s) 482
AgsI TTSAA 1 cut(s) 77
AjnI CCWGG 2 cut(s) 91, 679
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 1 cut(s) 576
AlwI GGATC 2 cut(s) 137, 150
AlwNI CAGNNNCTG 1 cut(s) 227
AoxI GGCC 3 cut(s) 235, 486, 576
ApeKI GCWGC 1 cut(s) 560
ApoI RAATTY 1 cut(s) 428
AspA2I CCTAGG 1 cut(s) 56
AspLEI GCGC 1 cut(s) 313
AspS9I GGNCC 4 cut(s) 8, 96, 218, 236
AsuC2I CCSGG 1 cut(s) 100
AsuHPI GGTGA 2 cut(s) 329, 383
AvaII GGWCC 3 cut(s) 8, 96, 218
AvrII CCTAGG 1 cut(s) 56
BaeGI GKGCMC 1 cut(s) 105
BalI TGGCCA 1 cut(s) 578
BamHI GGATCC 1 cut(s) 142
BanI GGYRCC 2 cut(s) 102, 173
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 1 cut(s) 547
BccI CCATC 1 cut(s) 48
BceAI ACGGC 1 cut(s) 541
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BciT130I CCWGG 2 cut(s) 93, 681
BcnI CCSGG 1 cut(s) 100
BcoDI GTCTC 1 cut(s) 576
BfaI CTAG 2 cut(s) 57, 107
BfrI CTTAAG 1 cut(s) 482
BglII AGATCT 2 cut(s) 33, 657
BisI GCNGC 1 cut(s) 561
BlnI CCTAGG 1 cut(s) 56
BlsI GCNGC 1 cut(s) 562
Bme1390I CCNGG 3 cut(s) 93, 100, 681
Bme18I GGWCC 3 cut(s) 8, 96, 218
BmgT120I GGNCC 4 cut(s) 8, 96, 218, 236
BmiI GGNNCC 5 cut(s) 10, 97, 104, 144, 175
BmrFI CCNGG 3 cut(s) 93, 100, 681
BpuMI CCSGG 1 cut(s) 100
BsaAI YACGTR 1 cut(s) 183
BsaJI CCNNGG 4 cut(s) 56, 92, 394, 680
BsaXI ACNNNNNCTCC 4 cut(s) 172, 202, 661, 691
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 227, 680
Bse1I ACTGG 2 cut(s) 153, 221
Bse3DI GCAATG 1 cut(s) 271
BseBI CCWGG 2 cut(s) 93, 681
BseDI CCNNGG 4 cut(s) 56, 92, 394, 680
BseGI GGATG 2 cut(s) 34, 422
BseLI CCNNNNNNNGG 3 cut(s) 98, 227, 680
BseMI GCAATG 1 cut(s) 271
BseNI ACTGG 2 cut(s) 153, 221
BseRI GAGGAG 2 cut(s) 663, 666
BseSI GKGCMC 1 cut(s) 105
BseXI GCAGC 1 cut(s) 547
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 3 cut(s) 237, 488, 578
BshNI GGYRCC 2 cut(s) 102, 173
BsiHKAI GWGCWC 1 cut(s) 285
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 2 cut(s) 109, 356
BslI CCNNNNNNNGG 3 cut(s) 98, 227, 680
BsmAI GTCTC 1 cut(s) 576
BsmFI GGGAC 2 cut(s) 109, 356
BsmI GAATGC 1 cut(s) 542
BsnI GGCC 3 cut(s) 237, 488, 578
Bsp1286I GDGCHC 2 cut(s) 105, 285
Bsp1407I TGTACA 1 cut(s) 246
Bsp143I GATC 3 cut(s) 33, 142, 657
Bsp19I CCATGG 1 cut(s) 394
BspACI CCGC 1 cut(s) 137
BspANI GGCC 3 cut(s) 237, 488, 578
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 5 cut(s) 10, 97, 104, 144, 175
BspPI GGATC 2 cut(s) 137, 150
BspT107I GGYRCC 2 cut(s) 102, 173
BspTI CTTAAG 1 cut(s) 482
BsrDI GCAATG 1 cut(s) 271
BsrGI TGTACA 1 cut(s) 246
BsrI ACTGG 2 cut(s) 153, 221
BssECI CCNNGG 4 cut(s) 56, 92, 394, 680
BssMI GATC 3 cut(s) 33, 142, 657
BssNAI GTATAC 1 cut(s) 186
BssT1I CCWWGG 2 cut(s) 56, 394
Bst1107I GTATAC 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 93, 681
Bst4CI ACNGT 2 cut(s) 72, 628
BstAFI CTTAAG 1 cut(s) 482
BstAUI TGTACA 1 cut(s) 246
BstBAI YACGTR 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 19, 172
BstDEI CTNAG 2 cut(s) 276, 361
BstDSI CCRYGG 1 cut(s) 394
BstF5I GGATG 2 cut(s) 34, 422
BstFNI CGCG 1 cut(s) 311
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 3 cut(s) 36, 145, 660
BstMAI GTCTC 1 cut(s) 576
BstMBI GATC 3 cut(s) 33, 142, 657
BstNI CCWGG 2 cut(s) 93, 681
BstNSI RCATGY 1 cut(s) 170
BstSCI CCNGG 3 cut(s) 91, 98, 679
BstSLI GKGCMC 1 cut(s) 105
BstSNI TACGTA 1 cut(s) 183
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 1 cut(s) 547
BstX2I RGATCY 3 cut(s) 33, 142, 657
BstYI RGATCY 3 cut(s) 33, 142, 657
BstZ17I GTATAC 1 cut(s) 186
BsuRI GGCC 3 cut(s) 237, 488, 578
BtgI CCRYGG 1 cut(s) 394
BtsCI GGATG 2 cut(s) 34, 422
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 3 cut(s) 146, 228, 483
Cac8I GCNNGC 2 cut(s) 19, 172
CaiI CAGNNNCTG 1 cut(s) 227
CfoI GCGC 1 cut(s) 313
Cfr13I GGNCC 4 cut(s) 8, 96, 218, 236
CseI GACGC 1 cut(s) 23
Csp6I GTAC 2 cut(s) 247, 453
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 3 cut(s) 167, 368, 395
CviJI RGCY 9 cut(s) 5, 21, 83, 227, 237, 399, 488, 578, 711
CviKI_1 RGCY 9 cut(s) 5, 21, 83, 227, 237, 399, 488, 578, 711
CviQI GTAC 2 cut(s) 247, 453
DdeI CTNAG 2 cut(s) 276, 361
DpnI GATC 3 cut(s) 35, 144, 659
DpnII GATC 3 cut(s) 33, 142, 657
DraIII CACNNNGTG 1 cut(s) 380
EaeI YGGCCR 1 cut(s) 576
Eco105I TACGTA 1 cut(s) 183
Eco130I CCWWGG 2 cut(s) 56, 394
Eco47I GGWCC 3 cut(s) 8, 96, 218
Eco57I CTGAAG 2 cut(s) 69, 184
EcoO109I RGGNCCY 1 cut(s) 236
EcoRII CCWGG 2 cut(s) 91, 679
EcoT14I CCWWGG 2 cut(s) 56, 394
ErhI CCWWGG 2 cut(s) 56, 394
FaeI CATG 3 cut(s) 170, 371, 398
FaqI GGGAC 2 cut(s) 109, 356
FatI CATG 3 cut(s) 166, 367, 394
FauNDI CATATG 1 cut(s) 532
FblI GTMKAC 2 cut(s) 185, 195
Fnu4HI GCNGC 1 cut(s) 561
FokI GGATG 2 cut(s) 41, 429
Fsp4HI GCNGC 1 cut(s) 561
FspBI CTAG 2 cut(s) 57, 107
GlaI GCGC 1 cut(s) 312
GluI GCNGC 1 cut(s) 561
HaeIII GGCC 3 cut(s) 237, 488, 578
HapII CCGG 1 cut(s) 99
HgaI GACGC 1 cut(s) 23
HhaI GCGC 1 cut(s) 313
Hin1II CATG 3 cut(s) 170, 371, 398
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 2 cut(s) 553, 613
HpaII CCGG 1 cut(s) 99
HphI GGTGA 2 cut(s) 329, 383
Hpy166II GTNNAC 4 cut(s) 186, 196, 337, 695
Hpy188I TCNGA 3 cut(s) 156, 215, 652
Hpy8I GTNNAC 4 cut(s) 186, 196, 337, 695
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 3 cut(s) 249, 538, 701
HpyCH4III ACNGT 2 cut(s) 72, 628
HpyCH4IV ACGT 1 cut(s) 182
HpyCH4V TGCA 4 cut(s) 162, 170, 264, 383
HpyF3I CTNAG 2 cut(s) 276, 361
HpySE526I ACGT 1 cut(s) 182
Hsp92II CATG 3 cut(s) 170, 371, 398
HspAI GCGC 1 cut(s) 311
Kzo9I GATC 3 cut(s) 33, 142, 657
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 1 cut(s) 547
MaeI CTAG 2 cut(s) 57, 107
MaeII ACGT 1 cut(s) 182
MalI GATC 3 cut(s) 35, 144, 659
MboI GATC 3 cut(s) 33, 142, 657
MflI RGATCY 3 cut(s) 33, 142, 657
MhlI GDGCHC 2 cut(s) 105, 285
MlsI TGGCCA 1 cut(s) 578
MluCI AATT 3 cut(s) 408, 428, 572
MluNI TGGCCA 1 cut(s) 578
MmeI TCCRAC 1 cut(s) 692
MnlI CCTC 9 cut(s) 133, 209, 227, 297, 430, 466, 582, 684, 687
Mox20I TGGCCA 1 cut(s) 578
MscI TGGCCA 1 cut(s) 578
MseI TTAA 2 cut(s) 432, 483
MslI CAYNNNNRTG 2 cut(s) 399, 535
Msp20I TGGCCA 1 cut(s) 578
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 3 cut(s) 93, 100, 681
Mva1269I GAATGC 1 cut(s) 542
MvaI CCWGG 2 cut(s) 93, 681
MvnI CGCG 1 cut(s) 311
NciI CCSGG 1 cut(s) 100
NcoI CCATGG 1 cut(s) 394
NdeI CATATG 1 cut(s) 532
NdeII GATC 3 cut(s) 33, 142, 657
NlaIII CATG 3 cut(s) 170, 371, 398
NlaIV GGNNCC 5 cut(s) 10, 97, 104, 144, 175
NspI RCATGY 1 cut(s) 170
PctI GAATGC 1 cut(s) 542
PfeI GAWTC 2 cut(s) 553, 613
PflFI GACNNNGTC 1 cut(s) 368
PflMI CCANNNNNTGG 1 cut(s) 227
PkrI GCNGC 1 cut(s) 562
Ppu21I YACGTR 1 cut(s) 183
PsiI TTATAA 1 cut(s) 330
Psp6I CCWGG 2 cut(s) 91, 679
PspGI CCWGG 2 cut(s) 91, 679
PspN4I GGNNCC 5 cut(s) 10, 97, 104, 144, 175
PspPI GGNCC 4 cut(s) 8, 96, 218, 236
PstNI CAGNNNCTG 1 cut(s) 227
PsuI RGATCY 3 cut(s) 33, 142, 657
PsyI GACNNNGTC 1 cut(s) 368
RsaI GTAC 2 cut(s) 248, 454
RsaNI GTAC 2 cut(s) 247, 453
RseI CAYNNNNRTG 2 cut(s) 399, 535
SalI GTCGAC 1 cut(s) 194
SaqAI TTAA 2 cut(s) 432, 483
SatI GCNGC 1 cut(s) 561
Sau3AI GATC 3 cut(s) 33, 142, 657
Sau96I GGNCC 4 cut(s) 8, 96, 218, 236
ScrFI CCNGG 3 cut(s) 93, 100, 681
SduI GDGCHC 2 cut(s) 105, 285
SetI ASST 7 cut(s) 23, 108, 135, 179, 185, 336, 458
SinI GGWCC 3 cut(s) 8, 96, 218
SmiMI CAYNNNNRTG 2 cut(s) 399, 535
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
SnaBI TACGTA 1 cut(s) 183
Sse9I AATT 3 cut(s) 408, 428, 572
SsiI CCGC 1 cut(s) 137
SspMI CTAG 2 cut(s) 57, 107
StyD4I CCNGG 3 cut(s) 91, 98, 679
StyI CCWWGG 2 cut(s) 56, 394
TaaI ACNGT 2 cut(s) 72, 628
TaiI ACGT 1 cut(s) 185
TaqI TCGA 2 cut(s) 126, 195
TasI AATT 3 cut(s) 408, 428, 572
TatI WGTACW 1 cut(s) 246
TfiI GAWTC 2 cut(s) 553, 613
Tru1I TTAA 2 cut(s) 432, 483
Tru9I TTAA 2 cut(s) 432, 483
TscAI CASTG 3 cut(s) 153, 228, 483
TseI GCWGC 1 cut(s) 560
TspDTI ATGAA 7 cut(s) 65, 80, 417, 441, 549, 636, 716
TspRI CASTG 3 cut(s) 153, 228, 483
Tth111I GACNNNGTC 1 cut(s) 368
Van91I CCANNNNNTGG 1 cut(s) 227
Vha464I CTTAAG 1 cut(s) 482
VpaK11BI GGWCC 3 cut(s) 8, 96, 218
XapI RAATTY 1 cut(s) 428
XceI RCATGY 1 cut(s) 170
XcmI CCANNNNNNNNNTGG 1 cut(s) 571
XmaJI CCTAGG 1 cut(s) 56
XmiI GTMKAC 2 cut(s) 185, 195
XspI CTAG 2 cut(s) 57, 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.