Rroxscaffold_1G00062410

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001608
Physical Location & Seq
Reverse (-)
84462937 .. 84463824
888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00062410.1

Sequence Viewer

Length: 327 bp
ATGATGAAGGAAAAGATGTGGTATGTGATATCTCAAAATGAGCCATCACTTCTTCCAATATCCTTGGCTTCAAAATTGTTGACCGAGGACTGCATGGCATGTGTTGATCTTCTTGAAGTCATGCTGGTGCAACATTTGCAGAGCAGCAGCAGCACAAATCTAAAAGTGATGGATCGGAGAGTGAGGGTGGTAGAGTTGGTCTTAAGGTTTCTCATCTATGGTCTCGGACTCGCCGTTGCTCTTCTTGTGGCCACTAATACTCGGGCCAAAGAGATCTTCATGATCAAGAAGAAAGCCAAGTTCACGAACATGAAGGCTCTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

108

Amino Acids

12.26

Weight (kDa)

9.59

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 59 - 108 2.7e-07 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 180
AcoI YGGCCR 1 cut(s) 249
AflII CTTAAG 1 cut(s) 202
AgsI TTSAA 2 cut(s) 72, 116
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 1 cut(s) 180
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 2 cut(s) 249, 264
ApeKI GCWGC 3 cut(s) 144, 147, 150
AspS9I GGNCC 1 cut(s) 264
AvaI CYCGRG 1 cut(s) 261
BalI TGGCCA 1 cut(s) 251
BbvI GCAGC 3 cut(s) 156, 159, 162
BccI CCATC 2 cut(s) 52, 163
BceAI ACGGC 1 cut(s) 218
BclI TGATCA 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 227
BfrI CTTAAG 1 cut(s) 202
BglII AGATCT 1 cut(s) 273
BisI GCNGC 3 cut(s) 145, 148, 151
BlsI GCNGC 3 cut(s) 146, 149, 152
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 63, 84
BseDI CCNNGG 2 cut(s) 63, 84
BseXI GCAGC 3 cut(s) 156, 159, 162
BshFI GGCC 2 cut(s) 251, 266
BsiHKCI CYCGRG 1 cut(s) 261
BsmAI GTCTC 1 cut(s) 227
BsnI GGCC 2 cut(s) 251, 266
Bso31I GGTCTC 1 cut(s) 227
BsoBI CYCGRG 1 cut(s) 261
Bsp143I GATC 4 cut(s) 106, 172, 273, 282
BspANI GGCC 2 cut(s) 251, 266
BspHI TCATGA 1 cut(s) 279
BspPI GGATC 1 cut(s) 180
BspQI GCTCTTC 1 cut(s) 246
BspTI CTTAAG 1 cut(s) 202
BspTNI GGTCTC 1 cut(s) 227
BssECI CCNNGG 2 cut(s) 63, 84
BssMI GATC 4 cut(s) 106, 172, 273, 282
BssT1I CCWWGG 1 cut(s) 63
Bst6I CTCTTC 1 cut(s) 246
BstAFI CTTAAG 1 cut(s) 202
BstAPI GCANNNNNTGC 1 cut(s) 136
BstKTI GATC 4 cut(s) 109, 175, 276, 285
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 4 cut(s) 106, 172, 273, 282
BstMWI GCNNNNNNNGC 2 cut(s) 136, 150
BstNSI RCATGY 1 cut(s) 102
BstV1I GCAGC 3 cut(s) 156, 159, 162
BstX2I RGATCY 1 cut(s) 273
BstYI RGATCY 1 cut(s) 273
BsuRI GGCC 2 cut(s) 251, 266
CciI TCATGA 1 cut(s) 279
Cfr13I GGNCC 1 cut(s) 264
CviAII CATG 5 cut(s) 94, 99, 121, 280, 310
CviJI RGCY 6 cut(s) 43, 68, 251, 266, 296, 317
CviKI_1 RGCY 6 cut(s) 43, 68, 251, 266, 296, 317
DpnI GATC 4 cut(s) 108, 174, 275, 284
DpnII GATC 4 cut(s) 106, 172, 273, 282
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 63
Eco31I GGTCTC 1 cut(s) 227
Eco32I GATATC 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 261
EcoRV GATATC 1 cut(s) 30
EcoT14I CCWWGG 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 63
FaeI CATG 5 cut(s) 97, 102, 124, 283, 313
FaiI YATR 7 cut(s) 24, 95, 100, 122, 219, 281, 311
FatI CATG 5 cut(s) 93, 98, 120, 279, 309
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 3 cut(s) 145, 148, 151
Fsp4HI GCNGC 3 cut(s) 145, 148, 151
GluI GCNGC 3 cut(s) 145, 148, 151
HaeIII GGCC 2 cut(s) 251, 266
Hin1II CATG 5 cut(s) 97, 102, 124, 283, 313
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HinfI GANTC 1 cut(s) 228
Hpy166II GTNNAC 2 cut(s) 81, 303
Hpy188I TCNGA 2 cut(s) 177, 227
Hpy188III TCNNGA 4 cut(s) 113, 280, 286, 304
Hpy8I GTNNAC 2 cut(s) 81, 303
HpyAV CCTTC 1 cut(s) 307
HpyCH4V TGCA 3 cut(s) 93, 130, 139
HpyF10VI GCNNNNNNNGC 2 cut(s) 136, 150
Hsp92II CATG 5 cut(s) 97, 102, 124, 283, 313
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 4 cut(s) 106, 172, 273, 282
LguI GCTCTTC 1 cut(s) 246
LpnPI CCDG 1 cut(s) 110
Lsp1109I GCAGC 3 cut(s) 156, 159, 162
MalI GATC 4 cut(s) 108, 174, 275, 284
MboI GATC 4 cut(s) 106, 172, 273, 282
MboII GAAGA 5 cut(s) 44, 101, 233, 268, 301
MflI RGATCY 1 cut(s) 273
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 1 cut(s) 74
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 1 cut(s) 222
MnlI CCTC 2 cut(s) 79, 177
Mox20I TGGCCA 1 cut(s) 251
MscI TGGCCA 1 cut(s) 251
MseI TTAA 1 cut(s) 203
MslI CAYNNNNRTG 2 cut(s) 125, 308
Msp20I TGGCCA 1 cut(s) 251
MspCI CTTAAG 1 cut(s) 202
MwoI GCNNNNNNNGC 2 cut(s) 136, 150
NdeII GATC 4 cut(s) 106, 172, 273, 282
NlaIII CATG 5 cut(s) 97, 102, 124, 283, 313
NspI RCATGY 1 cut(s) 102
PagI TCATGA 1 cut(s) 279
PciSI GCTCTTC 1 cut(s) 246
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PkrI GCNGC 3 cut(s) 146, 149, 152
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
PspPI GGNCC 1 cut(s) 264
PsuI RGATCY 1 cut(s) 273
RseI CAYNNNNRTG 2 cut(s) 125, 308
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 1 cut(s) 203
SatI GCNGC 3 cut(s) 145, 148, 151
Sau3AI GATC 4 cut(s) 106, 172, 273, 282
Sau96I GGNCC 1 cut(s) 264
SchI GAGTC 1 cut(s) 222
SetI ASST 1 cut(s) 209
SmiMI CAYNNNNRTG 2 cut(s) 125, 308
SmlI CTYRAG 1 cut(s) 202
SmoI CTYRAG 1 cut(s) 202
Sse9I AATT 1 cut(s) 74
StyI CCWWGG 1 cut(s) 63
TaqII GACCGA 1 cut(s) 98
TasI AATT 1 cut(s) 74
Tru1I TTAA 1 cut(s) 203
Tru9I TTAA 1 cut(s) 203
TseI GCWGC 3 cut(s) 144, 147, 150
TspDTI ATGAA 3 cut(s) 20, 268, 326
Vha464I CTTAAG 1 cut(s) 202
XceI RCATGY 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.