Rh5DG529800

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001608
Physical Location & Seq
Forward (+)
81739811 .. 81741995
2185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG529800.1

Sequence Viewer

Length: 495 bp
ATGTGCAACCCTCAGCTAAACAAGGACACGAAGGTTGATTTTCCAGAGGCTGGCTTTAACTCAGAGGGAGAGAAAGACTTGGAAGATTCTCTTTTTTCAGTTTTGGCTTCGAACTTAAATGAAGAAATTACAAAGTTCATAAGAAGGTCCTGGCTGGAGATATGGAAGAGATTTAGTATCAGTGACATTGTTTATAGAGATATGGCCCTTAAAGTCCACCTGAACATGTGTAATGAGTTCTGCTTGATTGTGCTCTTGCTTGCGGTGAGAGATAAAGAAGGGATTCCTGCGTTGGAAGATGTGGGTGAAGGAGTAATAAGTGAGGAAAAGATAGTGGGGAGGGAGTTGTTGGAGCTTTTGAGAGATGTTAAGGACCATTTCATGAAGGCCTATGATTCTGGTTTGGATGTTTCAAGGATGCTAGAGACTAACATGGTTCAGATGCTATCTGCATTGGAGGAGATTGAAGAAGGCATTAGAGAGAACCATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

164

Amino Acids

18.88

Weight (kDa)

4.59

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 116 - 157 2.3e-06 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 50
AciI CCGC 1 cut(s) 263
AfiI CCNNNNNNNGG 1 cut(s) 50
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 2 cut(s) 414, 467
AjnI CCWGG 1 cut(s) 149
AluBI AGCT 2 cut(s) 16, 355
AluI AGCT 2 cut(s) 16, 355
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 1 cut(s) 419
AlwNI CAGNNNCTG 1 cut(s) 50
AoxI GGCC 2 cut(s) 204, 387
Asp700I GAANNNNTTC 1 cut(s) 282
AspS9I GGNCC 3 cut(s) 147, 205, 373
AsuHPI GGTGA 2 cut(s) 277, 317
AsuII TTCGAA 1 cut(s) 110
AvaII GGWCC 2 cut(s) 147, 373
Bbv12I GWGCWC 1 cut(s) 255
BbvCI CCTCAGC 1 cut(s) 12
BciT130I CCWGG 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 1 cut(s) 422
Bme1390I CCNGG 1 cut(s) 151
Bme18I GGWCC 2 cut(s) 147, 373
BmgT120I GGNCC 3 cut(s) 147, 205, 373
BmrFI CCNGG 1 cut(s) 151
BmsI GCATC 2 cut(s) 408, 432
BpmI CTGGAG 1 cut(s) 176
Bpu10I CCTNAGC 1 cut(s) 12
Bpu14I TTCGAA 1 cut(s) 110
Bsc4I CCNNNNNNNGG 1 cut(s) 50
BseBI CCWGG 1 cut(s) 151
BseGI GGATG 2 cut(s) 412, 423
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMII CTCAG 2 cut(s) 26, 75
BseRI GAGGAG 1 cut(s) 473
BshFI GGCC 2 cut(s) 206, 389
BsiHKAI GWGCWC 1 cut(s) 255
BslI CCNNNNNNNGG 1 cut(s) 50
BsmAI GTCTC 1 cut(s) 419
BsnI GGCC 2 cut(s) 206, 389
Bsp119I TTCGAA 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 255
BspACI CCGC 1 cut(s) 263
BspANI GGCC 2 cut(s) 206, 389
BspCNI CTCAG 2 cut(s) 25, 74
BspHI TCATGA 1 cut(s) 381
BspT104I TTCGAA 1 cut(s) 110
Bst2UI CCWGG 1 cut(s) 151
Bst6I CTCTTC 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 2 cut(s) 52, 261
BstDEI CTNAG 2 cut(s) 12, 61
BstF5I GGATG 2 cut(s) 412, 423
BstMAI GTCTC 1 cut(s) 419
BstNI CCWGG 1 cut(s) 151
BstNSI RCATGY 1 cut(s) 229
BstSCI CCNGG 1 cut(s) 149
BsuRI GGCC 2 cut(s) 206, 389
BtsCI GGATG 2 cut(s) 412, 423
BtsIMutI CAGTG 1 cut(s) 187
Cac8I GCNNGC 2 cut(s) 52, 261
CaiI CAGNNNCTG 1 cut(s) 50
CciI TCATGA 1 cut(s) 381
Cfr13I GGNCC 3 cut(s) 147, 205, 373
CviAII CATG 4 cut(s) 226, 382, 433, 488
CviJI RGCY 8 cut(s) 16, 50, 54, 107, 154, 206, 355, 389
CviKI_1 RGCY 8 cut(s) 16, 50, 54, 107, 154, 206, 355, 389
DdeI CTNAG 2 cut(s) 12, 61
Eam1104I CTCTTC 1 cut(s) 161
EarI CTCTTC 1 cut(s) 161
Eco147I AGGCCT 1 cut(s) 389
Eco47I GGWCC 2 cut(s) 147, 373
EcoO109I RGGNCCY 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 149
FaeI CATG 4 cut(s) 229, 385, 436, 491
FaiI YATR 9 cut(s) 140, 163, 195, 203, 227, 383, 393, 434, 489
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FatI CATG 4 cut(s) 225, 381, 432, 487
FokI GGATG 2 cut(s) 419, 430
FspBI CTAG 1 cut(s) 422
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 2 cut(s) 206, 389
Hin1II CATG 4 cut(s) 229, 385, 436, 491
HinfI GANTC 3 cut(s) 86, 283, 395
HphI GGTGA 2 cut(s) 277, 317
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 2 cut(s) 64, 441
Hpy188III TCNNGA 2 cut(s) 44, 382
Hpy8I GTNNAC 1 cut(s) 217
HpyAV CCTTC 6 cut(s) 25, 138, 272, 302, 379, 464
HpyCH4V TGCA 2 cut(s) 6, 452
HpyF3I CTNAG 2 cut(s) 12, 61
Hsp92II CATG 4 cut(s) 229, 385, 436, 491
LmnI GCTCC 1 cut(s) 352
LpnPI CCDG 8 cut(s) 36, 57, 136, 140, 163, 233, 300, 384
LweI GCATC 2 cut(s) 408, 432
MaeI CTAG 1 cut(s) 422
MaeIII GTNAC 1 cut(s) 182
MboII GAAGA 5 cut(s) 95, 134, 178, 308, 479
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 1 cut(s) 126
MmeI TCCRAC 2 cut(s) 273, 330
MnlI CCTC 6 cut(s) 21, 40, 58, 316, 333, 451
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 4 cut(s) 57, 116, 210, 369
MspR9I CCNGG 1 cut(s) 151
MvaI CCWGG 1 cut(s) 151
NlaIII CATG 4 cut(s) 229, 385, 436, 491
NmuCI GTSAC 1 cut(s) 182
NspI RCATGY 1 cut(s) 229
NspV TTCGAA 1 cut(s) 110
PagI TCATGA 1 cut(s) 381
PceI AGGCCT 1 cut(s) 389
PciI ACATGT 1 cut(s) 225
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 3 cut(s) 86, 283, 395
PflMI CCANNNNNTGG 1 cut(s) 50
PpuMI RGGWCCY 1 cut(s) 147
PscI ACATGT 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 147
Psp6I CCWGG 1 cut(s) 149
PspGI CCWGG 1 cut(s) 149
PspPI GGNCC 3 cut(s) 147, 205, 373
PspPPI RGGWCCY 1 cut(s) 147
PstNI CAGNNNCTG 1 cut(s) 50
SaqAI TTAA 4 cut(s) 57, 116, 210, 369
Sau96I GGNCC 3 cut(s) 147, 205, 373
ScrFI CCNGG 1 cut(s) 151
SduI GDGCHC 1 cut(s) 255
SetI ASST 5 cut(s) 18, 36, 149, 222, 357
SfaNI GCATC 2 cut(s) 408, 432
SfuI TTCGAA 1 cut(s) 110
SinI GGWCC 2 cut(s) 147, 373
Sse9I AATT 1 cut(s) 126
SseBI AGGCCT 1 cut(s) 389
SsiI CCGC 1 cut(s) 263
SspMI CTAG 1 cut(s) 422
StuI AGGCCT 1 cut(s) 389
StyD4I CCNGG 1 cut(s) 149
TaqI TCGA 1 cut(s) 110
TasI AATT 1 cut(s) 126
TfiI GAWTC 3 cut(s) 86, 283, 395
Tru1I TTAA 4 cut(s) 57, 116, 210, 369
Tru9I TTAA 4 cut(s) 57, 116, 210, 369
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 1 cut(s) 182
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 4 cut(s) 127, 135, 370, 398
TspRI CASTG 1 cut(s) 187
Van91I CCANNNNNTGG 1 cut(s) 50
VpaK11BI GGWCC 2 cut(s) 147, 373
XceI RCATGY 1 cut(s) 229
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.