Rh7DG258400

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001608
Physical Location & Seq
Forward (+)
27131256 .. 27146389
15134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG258400.1

Sequence Viewer

Length: 579 bp
ATGCATGAAAATAATTATCGGCTTGACATGTCCCTCTTAGCTGGGTGTGATTTTACTTGGCACCAACAAGGCTCTAGACTTTTGAAGTATCGGGCTCGTGGTCAACTGATCTTGCTCTTGGAGACTTGGTTCGATTCAGAAATTTTCAAGCTCGGATACAAGAGTATGTTGAAGCTACAAGCTTCTGTCAATTCTGGAGTGAGAGATAAAGAAGGGATTCCTGTGTTGGAAGATGTGGGTGAAGGAGTAATAAGTGAGGAAAAGATAGTGGGGAGGGAGTTGTTGGAGCTTTTGAGAGATGTTAAGGACCATTTCATGAAGGCCTATGATTCTGGTTTGGATGTTTCAAGGATGCTGCTAGTGTCTAACATGGTTCAGATGCTATCTGCATTGGAGGAGATTGAAGAAGTAGAAGAAAGAAGAGAGGAGCAGCAGGAACAGGCATATTACATCAGTGGTGGCAGAAGTAGAAGAAAGAAAAGAGGGGCAACAGGAGCAAGCATTAGTAGTGGGCGAAGTGGGCAAGCAATCATAAATAGTCCAATCATCACTATCCTTAGGATGCAGGATAGGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

192

Amino Acids

21.87

Weight (kDa)

6.0

Isoelectric Point (pI)

56.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 94 - 160 6.6e-06 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 60
AcsI RAATTY 1 cut(s) 141
AflIII ACRYGT 1 cut(s) 27
AgsI TTSAA 5 cut(s) 85, 148, 172, 348, 404
AloI GAACNNNNNNTCC 2 cut(s) 113, 145
AluBI AGCT 5 cut(s) 41, 151, 175, 182, 289
AluI AGCT 5 cut(s) 41, 151, 175, 182, 289
Alw26I GTCTC 1 cut(s) 116
AoxI GGCC 1 cut(s) 321
ApeKI GCWGC 2 cut(s) 355, 430
ApoI RAATTY 1 cut(s) 141
Asp700I GAANNNNTTC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 307
AsuHPI GGTGA 1 cut(s) 251
AvaII GGWCC 1 cut(s) 307
AxyI CCTNAGG 1 cut(s) 557
BaeI ACNNNNGTAYC 2 cut(s) 148, 181
BanI GGYRCC 1 cut(s) 60
BanII GRGCYC 1 cut(s) 97
BauI CACGAG 1 cut(s) 96
BbvI GCAGC 2 cut(s) 342, 442
BciVI GTATCC 1 cut(s) 149
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 2 cut(s) 75, 359
BfuI GTATCC 1 cut(s) 149
BisI GCNGC 2 cut(s) 356, 431
BlsI GCNGC 2 cut(s) 357, 432
Bme18I GGWCC 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 307
BmiI GGNNCC 1 cut(s) 62
BmsI GCATC 3 cut(s) 342, 369, 552
BpmI CTGGAG 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bse21I CCTNAGG 1 cut(s) 557
BseGI GGATG 3 cut(s) 346, 357, 567
BseRI GAGGAG 2 cut(s) 410, 440
BseXI GCAGC 2 cut(s) 342, 442
BseYI CCCAGC 1 cut(s) 41
BshFI GGCC 1 cut(s) 323
BshNI GGYRCC 1 cut(s) 60
BslFI GGGAC 1 cut(s) 16
BsmAI GTCTC 1 cut(s) 116
BsmFI GGGAC 1 cut(s) 16
BsnI GGCC 1 cut(s) 323
Bsp1286I GDGCHC 1 cut(s) 97
Bsp143I GATC 1 cut(s) 108
BspANI GGCC 1 cut(s) 323
BspHI TCATGA 1 cut(s) 315
BspLI GGNNCC 1 cut(s) 62
BspT107I GGYRCC 1 cut(s) 60
BssMI GATC 1 cut(s) 108
BssSI CACGAG 1 cut(s) 96
Bst2BI CACGAG 1 cut(s) 96
Bst6I CTCTTC 1 cut(s) 415
BstC8I GCNNGC 2 cut(s) 499, 525
BstDEI CTNAG 2 cut(s) 37, 557
BstF5I GGATG 3 cut(s) 346, 357, 567
BstKTI GATC 1 cut(s) 111
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 1 cut(s) 108
BstMWI GCNNNNNNNGC 2 cut(s) 494, 520
BstNSI RCATGY 1 cut(s) 31
BstV1I GCAGC 2 cut(s) 342, 442
Bsu36I CCTNAGG 1 cut(s) 557
BsuI GTATCC 1 cut(s) 149
BsuRI GGCC 1 cut(s) 323
BtsCI GGATG 3 cut(s) 346, 357, 567
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 2 cut(s) 499, 525
CciI TCATGA 1 cut(s) 315
Cfr13I GGNCC 1 cut(s) 307
CviAII CATG 5 cut(s) 5, 28, 316, 370, 576
CviJI RGCY 9 cut(s) 22, 41, 72, 95, 151, 175, 182, 289, 323
CviKI_1 RGCY 9 cut(s) 22, 41, 72, 95, 151, 175, 182, 289, 323
DdeI CTNAG 2 cut(s) 37, 557
DpnI GATC 1 cut(s) 110
DpnII GATC 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 415
EarI CTCTTC 1 cut(s) 415
Eco147I AGGCCT 1 cut(s) 323
Eco24I GRGCYC 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 307
Eco81I CCTNAGG 1 cut(s) 557
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 97
FaeI CATG 5 cut(s) 8, 31, 319, 373, 579
FaiI YATR 9 cut(s) 6, 29, 167, 317, 327, 371, 445, 533, 577
FaqI GGGAC 1 cut(s) 16
FatI CATG 5 cut(s) 4, 27, 315, 369, 575
Fnu4HI GCNGC 2 cut(s) 356, 431
FokI GGATG 3 cut(s) 353, 364, 574
FriOI GRGCYC 1 cut(s) 97
Fsp4HI GCNGC 2 cut(s) 356, 431
FspBI CTAG 2 cut(s) 75, 359
GluI GCNGC 2 cut(s) 356, 431
GsaI CCCAGC 1 cut(s) 45
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 1 cut(s) 323
Hin1II CATG 5 cut(s) 8, 31, 319, 373, 579
HincII GTYRAC 1 cut(s) 104
HindII GTYRAC 1 cut(s) 104
HindIII AAGCTT 1 cut(s) 180
HinfI GANTC 3 cut(s) 134, 217, 329
HphI GGTGA 1 cut(s) 251
Hpy166II GTNNAC 1 cut(s) 104
Hpy188I TCNGA 3 cut(s) 139, 155, 378
Hpy188III TCNNGA 3 cut(s) 75, 195, 316
Hpy8I GTNNAC 1 cut(s) 104
HpyAV CCTTC 3 cut(s) 206, 236, 313
HpyCH4V TGCA 3 cut(s) 4, 389, 565
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 520
HpyF3I CTNAG 2 cut(s) 37, 557
Hsp92II CATG 5 cut(s) 8, 31, 319, 373, 579
Kzo9I GATC 1 cut(s) 108
LmnI GCTCC 3 cut(s) 286, 427, 494
LpnPI CCDG 8 cut(s) 27, 180, 234, 318, 419, 425, 477, 551
Lsp1109I GCAGC 2 cut(s) 342, 442
LweI GCATC 3 cut(s) 342, 369, 552
MaeI CTAG 2 cut(s) 75, 359
MalI GATC 1 cut(s) 110
MboI GATC 1 cut(s) 108
MboII GAAGA 5 cut(s) 242, 416, 425, 432, 483
MhlI GDGCHC 1 cut(s) 97
MluCI AATT 3 cut(s) 13, 141, 190
MmeI TCCRAC 2 cut(s) 207, 264
MnlI CCTC 6 cut(s) 44, 250, 267, 388, 418, 476
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 216
MseI TTAA 1 cut(s) 303
MwoI GCNNNNNNNGC 2 cut(s) 494, 520
NdeII GATC 1 cut(s) 108
NlaIII CATG 5 cut(s) 8, 31, 319, 373, 579
NlaIV GGNNCC 1 cut(s) 62
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 31
PagI TCATGA 1 cut(s) 315
PceI AGGCCT 1 cut(s) 323
PciI ACATGT 1 cut(s) 27
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 3 cut(s) 134, 217, 329
PkrI GCNGC 2 cut(s) 357, 432
PscI ACATGT 1 cut(s) 27
PspFI CCCAGC 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 62
PspPI GGNCC 1 cut(s) 307
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 2 cut(s) 356, 431
Sau3AI GATC 1 cut(s) 108
Sau96I GGNCC 1 cut(s) 307
SduI GDGCHC 1 cut(s) 97
SetI ASST 5 cut(s) 43, 153, 177, 184, 291
SfaNI GCATC 3 cut(s) 342, 369, 552
SinI GGWCC 1 cut(s) 307
Sse9I AATT 3 cut(s) 13, 141, 190
SseBI AGGCCT 1 cut(s) 323
SspMI CTAG 2 cut(s) 75, 359
StuI AGGCCT 1 cut(s) 323
TaqI TCGA 1 cut(s) 132
TasI AATT 3 cut(s) 13, 141, 190
TfiI GAWTC 3 cut(s) 134, 217, 329
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 1 cut(s) 460
TseI GCWGC 2 cut(s) 355, 430
TspDTI ATGAA 3 cut(s) 21, 304, 332
TspRI CASTG 1 cut(s) 460
VpaK11BI GGWCC 1 cut(s) 307
XapI RAATTY 1 cut(s) 141
XbaI TCTAGA 1 cut(s) 74
XceI RCATGY 1 cut(s) 31
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 2 cut(s) 75, 359
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.