Rroxscaffold_3G00221160

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001608
Physical Location & Seq
Forward (+)
3866808 .. 3876432
9625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00221160.1

Sequence Viewer

Length: 651 bp
ATGAAGGAAAAGACGTGGTATGTGATATCTCAAAATGAGCCATCACTTCTTCCAATATCCTTGGTAGGTATTCAAAGCTCTGTATCGGGCTCGTGGTCAGCTGATCTTGCTCTTGGAGACTTGGTTCGATTCAGAAATTTTCAAGCTCGGGCAGTGAGAGATAAAGAAGGGATTCCTGCATTGGAAGATGTGGGTGAAGGAGTAATAAGTGAGGAAAAGATAGTGGGGAGGGAGTTGTTGGAGCTTTTGAGAGATGTTAAGGACCATTTCATGAAGGCCTATGATTCTGGTTTGGATGTTTCAAGGATGCTAGAGTCTAACATGGTTCAGATGCTATCTGCATTGGAGGAGATTGAAGAAGTAGAAGAAAGAAGAGAGGAGCAGCAGGAGCAGGCATATTACATCAGTGGTGGCAAAAGTAGAAGAAAGAAAAGAGGGGCTGCAGGAGCAAGCATTAGTAGTGGGCGAAGTGGGCAAGCAATCATAAATAGTCCAATAATCACTTTCATACAAATAGCCAACAATGAAGATAGAAAGAGAAGTTGGTTGATCGAACAAGCAAACGAAGAGACCAGCTTGATTTTGCTTCTCCGATTGAAGAAGATTGAGCTTCCGGAAAATTTGATGCTTCCTTCATCTATTATTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

216

Amino Acids

24.29

Weight (kDa)

5.12

Isoelectric Point (pI)

62.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 79 - 143 2.5e-07 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 613
AcsI RAATTY 2 cut(s) 136, 619
AgsI TTSAA 5 cut(s) 74, 143, 303, 356, 598
AjiI CACGTC 1 cut(s) 15
AjuI GAANNNNNNNTTGG 2 cut(s) 526, 558
AloI GAACNNNNNNTCC 2 cut(s) 108, 140
AluBI AGCT 6 cut(s) 78, 101, 146, 244, 576, 610
AluI AGCT 6 cut(s) 78, 101, 146, 244, 576, 610
Alw26I GTCTC 2 cut(s) 111, 563
Ama87I CYCGRG 1 cut(s) 147
Aor13HI TCCGGA 1 cut(s) 613
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 2 cut(s) 382, 440
ApoI RAATTY 2 cut(s) 136, 619
Asp700I GAANNNNTTC 1 cut(s) 171
AspS9I GGNCC 1 cut(s) 262
AsuHPI GGTGA 1 cut(s) 206
AvaI CYCGRG 1 cut(s) 147
AvaII GGWCC 1 cut(s) 262
BanII GRGCYC 1 cut(s) 92
BauI CACGAG 1 cut(s) 91
BbvI GCAGC 2 cut(s) 394, 427
BccI CCATC 1 cut(s) 49
BcoDI GTCTC 2 cut(s) 111, 563
BfaI CTAG 1 cut(s) 311
BfmI CTRYAG 1 cut(s) 441
BisI GCNGC 2 cut(s) 383, 441
BlsI GCNGC 2 cut(s) 384, 442
Bme18I GGWCC 1 cut(s) 262
BmeT110I CYCGRG 1 cut(s) 147
BmgBI CACGTC 1 cut(s) 15
BmgT120I GGNCC 1 cut(s) 262
BmsI GCATC 3 cut(s) 297, 321, 615
BsaI GGTCTC 1 cut(s) 563
BsaJI CCNNGG 1 cut(s) 60
BsaWI WCCGGW 1 cut(s) 613
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
BseAI TCCGGA 1 cut(s) 613
BseDI CCNNGG 1 cut(s) 60
BseGI GGATG 2 cut(s) 301, 312
BseRI GAGGAG 2 cut(s) 362, 392
BseXI GCAGC 2 cut(s) 394, 427
BshFI GGCC 1 cut(s) 278
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 1 cut(s) 614
BsmAI GTCTC 2 cut(s) 111, 563
BsnI GGCC 1 cut(s) 278
Bso31I GGTCTC 1 cut(s) 563
BsoBI CYCGRG 1 cut(s) 147
Bsp1286I GDGCHC 1 cut(s) 92
Bsp13I TCCGGA 1 cut(s) 613
Bsp143I GATC 2 cut(s) 103, 549
BspANI GGCC 1 cut(s) 278
BspEI TCCGGA 1 cut(s) 613
BspHI TCATGA 1 cut(s) 270
BspMAI CTGCAG 1 cut(s) 445
BspTNI GGTCTC 1 cut(s) 563
BssECI CCNNGG 1 cut(s) 60
BssMI GATC 2 cut(s) 103, 549
BssSI CACGAG 1 cut(s) 91
BssT1I CCWWGG 1 cut(s) 60
Bst2BI CACGAG 1 cut(s) 91
Bst6I CTCTTC 2 cut(s) 367, 561
BstC8I GCNNGC 3 cut(s) 393, 451, 477
BstF5I GGATG 2 cut(s) 301, 312
BstKTI GATC 2 cut(s) 106, 552
BstMAI GTCTC 2 cut(s) 111, 563
BstMBI GATC 2 cut(s) 103, 549
BstMWI GCNNNNNNNGC 4 cut(s) 107, 388, 446, 472
BstSFI CTRYAG 1 cut(s) 441
BstV1I GCAGC 2 cut(s) 394, 427
BsuRI GGCC 1 cut(s) 278
BtrI CACGTC 1 cut(s) 15
BtsCI GGATG 2 cut(s) 301, 312
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 2 cut(s) 159, 412
Cac8I GCNNGC 3 cut(s) 393, 451, 477
CciI TCATGA 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 262
CviAII CATG 2 cut(s) 271, 322
DpnI GATC 2 cut(s) 105, 551
DpnII GATC 2 cut(s) 103, 549
Eam1104I CTCTTC 2 cut(s) 367, 561
EarI CTCTTC 2 cut(s) 367, 561
Eco130I CCWWGG 1 cut(s) 60
Eco147I AGGCCT 1 cut(s) 278
Eco24I GRGCYC 1 cut(s) 92
Eco31I GGTCTC 1 cut(s) 563
Eco32I GATATC 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 262
Eco88I CYCGRG 1 cut(s) 147
EcoRV GATATC 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 60
EcoT38I GRGCYC 1 cut(s) 92
ErhI CCWWGG 1 cut(s) 60
FaeI CATG 2 cut(s) 274, 325
FaiI YATR 7 cut(s) 21, 272, 282, 323, 397, 485, 509
FatI CATG 2 cut(s) 270, 321
Fnu4HI GCNGC 2 cut(s) 383, 441
FokI GGATG 2 cut(s) 308, 319
FriOI GRGCYC 1 cut(s) 92
Fsp4HI GCNGC 2 cut(s) 383, 441
FspBI CTAG 1 cut(s) 311
GluI GCNGC 2 cut(s) 383, 441
HaeIII GGCC 1 cut(s) 278
HapII CCGG 1 cut(s) 614
Hin1II CATG 2 cut(s) 274, 325
HinfI GANTC 4 cut(s) 129, 172, 284, 314
HpaII CCGG 1 cut(s) 614
HphI GGTGA 1 cut(s) 206
Hpy188I TCNGA 3 cut(s) 134, 330, 593
Hpy188III TCNNGA 2 cut(s) 271, 614
HpyAV CCTTC 4 cut(s) 161, 191, 268, 642
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 3 cut(s) 179, 341, 443
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 388, 446, 472
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 2 cut(s) 274, 325
Kpn2I TCCGGA 1 cut(s) 613
Kzo9I GATC 2 cut(s) 103, 549
LmnI GCTCC 4 cut(s) 241, 379, 388, 446
LpnPI CCDG 7 cut(s) 189, 273, 371, 377, 429, 586, 627
Lsp1109I GCAGC 2 cut(s) 394, 427
LweI GCATC 3 cut(s) 297, 321, 615
MaeI CTAG 1 cut(s) 311
MaeII ACGT 1 cut(s) 14
MalI GATC 2 cut(s) 105, 551
MboI GATC 2 cut(s) 103, 549
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 2 cut(s) 136, 619
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 5 cut(s) 205, 222, 340, 370, 428
MroI TCCGGA 1 cut(s) 613
MroXI GAANNNNTTC 1 cut(s) 171
MseI TTAA 1 cut(s) 258
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 1 cut(s) 614
MwoI GCNNNNNNNGC 4 cut(s) 107, 388, 446, 472
NdeII GATC 2 cut(s) 103, 549
NlaIII CATG 2 cut(s) 274, 325
PagI TCATGA 1 cut(s) 270
PceI AGGCCT 1 cut(s) 278
PdmI GAANNNNTTC 1 cut(s) 171
PfeI GAWTC 3 cut(s) 129, 172, 284
PkrI GCNGC 2 cut(s) 384, 442
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PspPI GGNCC 1 cut(s) 262
PstI CTGCAG 1 cut(s) 445
PvuII CAGCTG 1 cut(s) 101
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 2 cut(s) 383, 441
Sau3AI GATC 2 cut(s) 103, 549
Sau96I GGNCC 1 cut(s) 262
SchI GAGTC 1 cut(s) 323
SduI GDGCHC 1 cut(s) 92
SetI ASST 8 cut(s) 17, 70, 80, 103, 148, 246, 578, 612
SfaNI GCATC 3 cut(s) 297, 321, 615
SfcI CTRYAG 1 cut(s) 441
SinI GGWCC 1 cut(s) 262
Sse9I AATT 2 cut(s) 136, 619
SseBI AGGCCT 1 cut(s) 278
SspMI CTAG 1 cut(s) 311
StuI AGGCCT 1 cut(s) 278
StyI CCWWGG 1 cut(s) 60
TaiI ACGT 1 cut(s) 17
TaqI TCGA 2 cut(s) 127, 552
TasI AATT 2 cut(s) 136, 619
TfiI GAWTC 3 cut(s) 129, 172, 284
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 2 cut(s) 159, 412
TseI GCWGC 2 cut(s) 382, 440
TspDTI ATGAA 6 cut(s) 17, 259, 287, 496, 540, 624
TspRI CASTG 2 cut(s) 159, 412
VpaK11BI GGWCC 1 cut(s) 262
XapI RAATTY 2 cut(s) 136, 619
XmnI GAANNNNTTC 1 cut(s) 171
XspI CTAG 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.