Rroxscaffold_6G00423540

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001606
Physical Location & Seq
Reverse (-)
44640085 .. 44641945
1861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00423540.1

Sequence Viewer

Length: 540 bp
ATGAGGTTGTCGAGAGACGCCGGCGGGTTCTTGGGGCTCCGAGCGTGGAGGACCCGGGTTTCGGGTTCCGGGTCTAGCTTCGTGGCGATGGAGTTCGACACTCTCATAGACGTGGAGTTCAAGGACATTAATAACAACCACGTGGGCACGGATCTCAACTCCATGGTTTCGTCACGGGTCGGCGATCTTAGCGCTGCGGATATTGATCTCAAGAGCAGTACCCACGGCTTAGTGGTGCTCGGGTTTCCCAATCCGAGTGGTCGCCTTGGGACAACGGCGGGGAGTATGGATGTAAGGCCGGTGATGGGTTTTGGAGGACGGTGGTGCGGCTCGGGTTGTCGGCGTCGCTATGGACGGGGTGTGTCGGTGTTCCGCAAACCCATTCCAACCGGTGACGGTGGCAATGCGCACGGCTTTAGTTCCTTTGGGTCACCACGGCGGGCGGTTGGTATTGGTGTGGTTGTTGGTGGTGAGAACGGCGAAGATGGACGTGGTGGAGGCGAACTCTACCTCGAGCTTTTGACATTGGTTACGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase

Protein Analysis

179

Amino Acids

18.77

Weight (kDa)

9.23

Isoelectric Point (pI)

28.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 6 - 89 1.5e-12 Legume lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 408
AciI CCGC 7 cut(s) 24, 197, 278, 327, 373, 439, 443
AclWI GGATC 1 cut(s) 159
AcvI CACGTG 1 cut(s) 142
AcyI GRCGYC 2 cut(s) 18, 343
AfaI GTAC 1 cut(s) 220
AfeI AGCGCT 1 cut(s) 193
AfiI CCNNNNNNNGG 2 cut(s) 61, 305
AgeI ACCGGT 1 cut(s) 389
AgsI TTSAA 1 cut(s) 121
AjiI CACGTC 2 cut(s) 112, 491
AluBI AGCT 2 cut(s) 78, 517
AluI AGCT 2 cut(s) 78, 517
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 159
Ama87I CYCGRG 4 cut(s) 54, 239, 331, 512
Aor51HI AGCGCT 1 cut(s) 193
AoxI GGCC 1 cut(s) 296
ApeKI GCWGC 1 cut(s) 194
ArsI GACNNNNNNTTYG 2 cut(s) 43, 75
AseI ATTAAT 1 cut(s) 129
AsiGI ACCGGT 1 cut(s) 389
AspLEI GCGC 2 cut(s) 194, 409
AspS9I GGNCC 1 cut(s) 51
AsuC2I CCSGG 3 cut(s) 55, 56, 70
AsuHPI GGTGA 4 cut(s) 313, 404, 423, 482
AvaI CYCGRG 4 cut(s) 54, 239, 331, 512
AvaII GGWCC 1 cut(s) 51
BaeGI GKGCMC 1 cut(s) 149
BanII GRGCYC 1 cut(s) 39
BbrPI CACGTG 1 cut(s) 142
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 181
BccI CCATC 3 cut(s) 82, 298, 479
BceAI ACGGC 5 cut(s) 241, 291, 427, 452, 493
BcnI CCSGG 3 cut(s) 55, 56, 70
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 2 cut(s) 75, 538
BfoI RGCGCY 1 cut(s) 195
BisI GCNGC 2 cut(s) 195, 328
BlsI GCNGC 2 cut(s) 196, 329
Bme1390I CCNGG 3 cut(s) 55, 56, 70
Bme18I GGWCC 1 cut(s) 51
BmeT110I CYCGRG 4 cut(s) 54, 239, 331, 512
BmgBI CACGTC 2 cut(s) 112, 491
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 3 cut(s) 38, 53, 67
BmrFI CCNGG 3 cut(s) 55, 56, 70
BplI GAGNNNNNCTC 2 cut(s) 489, 521
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 3 cut(s) 55, 56, 70
BsaAI YACGTR 1 cut(s) 142
BsaBI GATNNNNATC 1 cut(s) 204
BsaHI GRCGYC 2 cut(s) 18, 343
BsaJI CCNNGG 5 cut(s) 54, 162, 223, 265, 434
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 305
Bse118I RCCGGY 3 cut(s) 20, 298, 389
Bse3DI GCAATG 1 cut(s) 409
Bse8I GATNNNNATC 1 cut(s) 204
BseDI CCNNGG 5 cut(s) 54, 162, 223, 265, 434
BseGI GGATG 1 cut(s) 295
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 2 cut(s) 61, 305
BseMI GCAATG 1 cut(s) 409
BseSI GKGCMC 1 cut(s) 149
BseXI GCAGC 1 cut(s) 181
BshFI GGCC 1 cut(s) 298
BshTI ACCGGT 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 240
BsiHKCI CYCGRG 4 cut(s) 54, 239, 331, 512
BsiSI CCGG 5 cut(s) 21, 55, 69, 299, 390
BslFI GGGAC 1 cut(s) 283
BslI CCNNNNNNNGG 2 cut(s) 61, 305
BsmAI GTCTC 1 cut(s) 9
BsmBI CGTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 283
BsnI GGCC 1 cut(s) 298
BsoBI CYCGRG 4 cut(s) 54, 239, 331, 512
Bsp1286I GDGCHC 3 cut(s) 39, 149, 240
Bsp143I GATC 4 cut(s) 151, 184, 205, 534
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 7 cut(s) 24, 197, 278, 327, 373, 439, 443
BspANI GGCC 1 cut(s) 298
BspLI GGNNCC 3 cut(s) 38, 53, 67
BspPI GGATC 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 409
BsrFI RCCGGY 3 cut(s) 20, 298, 389
BssAI RCCGGY 3 cut(s) 20, 298, 389
BssECI CCNNGG 5 cut(s) 54, 162, 223, 265, 434
BssMI GATC 4 cut(s) 151, 184, 205, 534
BssNI GRCGYC 2 cut(s) 18, 343
BssT1I CCWWGG 2 cut(s) 162, 265
Bst4CI ACNGT 2 cut(s) 321, 398
BstACI GRCGYC 2 cut(s) 18, 343
BstBAI YACGTR 1 cut(s) 142
BstC8I GCNNGC 2 cut(s) 22, 441
BstDEI CTNAG 2 cut(s) 188, 229
BstDSI CCRYGG 3 cut(s) 162, 223, 434
BstEII GGTNACC 1 cut(s) 429
BstF5I GGATG 1 cut(s) 295
BstH2I RGCGCY 1 cut(s) 195
BstHHI GCGC 2 cut(s) 194, 409
BstKTI GATC 4 cut(s) 154, 187, 208, 537
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 4 cut(s) 151, 184, 205, 534
BstMWI GCNNNNNNNGC 1 cut(s) 189
BstPI GGTNACC 1 cut(s) 429
BstSCI CCNGG 3 cut(s) 53, 54, 68
BstSLI GKGCMC 1 cut(s) 149
BstV1I GCAGC 1 cut(s) 181
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuRI GGCC 1 cut(s) 298
BtgI CCRYGG 3 cut(s) 162, 223, 434
BtgZI GCGATG 1 cut(s) 101
BtrI CACGTC 2 cut(s) 112, 491
BtsCI GGATG 1 cut(s) 295
Cac8I GCNNGC 2 cut(s) 22, 441
CfoI GCGC 2 cut(s) 194, 409
Cfr10I RCCGGY 3 cut(s) 20, 298, 389
Cfr13I GGNCC 1 cut(s) 51
Cfr9I CCCGGG 1 cut(s) 54
CseI GACGC 2 cut(s) 26, 332
Csp6I GTAC 1 cut(s) 219
CspAI ACCGGT 1 cut(s) 389
CspCI CAANNNNNGTGG 2 cut(s) 238, 273
CviAII CATG 1 cut(s) 163
CviJI RGCY 7 cut(s) 37, 78, 228, 298, 330, 414, 517
CviKI_1 RGCY 7 cut(s) 37, 78, 228, 298, 330, 414, 517
CviQI GTAC 1 cut(s) 219
DdeI CTNAG 2 cut(s) 188, 229
DpnI GATC 4 cut(s) 153, 186, 207, 536
DpnII GATC 4 cut(s) 151, 184, 205, 534
Eco130I CCWWGG 2 cut(s) 162, 265
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 51
Eco47III AGCGCT 1 cut(s) 193
Eco72I CACGTG 1 cut(s) 142
Eco88I CYCGRG 4 cut(s) 54, 239, 331, 512
Eco91I GGTNACC 1 cut(s) 429
EcoO109I RGGNCCY 1 cut(s) 51
EcoO65I GGTNACC 1 cut(s) 429
EcoT14I CCWWGG 2 cut(s) 162, 265
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 2 cut(s) 162, 265
Esp3I CGTCTC 1 cut(s) 9
FaeI CATG 1 cut(s) 166
FaiI YATR 4 cut(s) 107, 164, 287, 351
FaqI GGGAC 1 cut(s) 283
FatI CATG 1 cut(s) 162
FauI CCCGC 3 cut(s) 17, 271, 432
Fnu4HI GCNGC 2 cut(s) 195, 328
FokI GGATG 1 cut(s) 302
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 2 cut(s) 195, 328
FspAI RTGCGCAY 1 cut(s) 408
FspBI CTAG 2 cut(s) 75, 538
FspI TGCGCA 1 cut(s) 408
GlaI GCGC 2 cut(s) 193, 408
GluI GCNGC 2 cut(s) 195, 328
HaeII RGCGCY 1 cut(s) 195
HaeIII GGCC 1 cut(s) 298
HapII CCGG 5 cut(s) 21, 55, 69, 299, 390
HgaI GACGC 2 cut(s) 26, 332
HhaI GCGC 2 cut(s) 194, 409
Hin1I GRCGYC 2 cut(s) 18, 343
Hin1II CATG 1 cut(s) 166
Hin6I GCGC 2 cut(s) 192, 407
HinP1I GCGC 2 cut(s) 192, 407
HpaII CCGG 5 cut(s) 21, 55, 69, 299, 390
HphI GGTGA 4 cut(s) 313, 404, 423, 482
Hpy188I TCNGA 2 cut(s) 41, 255
Hpy188III TCNNGA 2 cut(s) 12, 211
Hpy99I CGWCG 1 cut(s) 348
HpyCH4III ACNGT 2 cut(s) 321, 398
HpyCH4IV ACGT 3 cut(s) 111, 141, 490
HpyF10VI GCNNNNNNNGC 1 cut(s) 189
HpyF3I CTNAG 2 cut(s) 188, 229
HpySE526I ACGT 3 cut(s) 111, 141, 490
Hsp92I GRCGYC 2 cut(s) 18, 343
Hsp92II CATG 1 cut(s) 166
HspAI GCGC 2 cut(s) 192, 407
KroI GCCGGC 1 cut(s) 20
KroNI GCCGGC 1 cut(s) 22
Kzo9I GATC 4 cut(s) 151, 184, 205, 534
LmnI GCTCC 1 cut(s) 42
LpnPI CCDG 5 cut(s) 34, 68, 82, 312, 403
Lsp1109I GCAGC 1 cut(s) 181
MaeI CTAG 2 cut(s) 75, 538
MaeII ACGT 3 cut(s) 111, 141, 490
MaeIII GTNAC 4 cut(s) 171, 392, 429, 529
MalI GATC 4 cut(s) 153, 186, 207, 536
MboI GATC 4 cut(s) 151, 184, 205, 534
MboII GAAGA 1 cut(s) 494
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 3 cut(s) 39, 149, 240
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 4 cut(s) 42, 308, 491, 521
MreI CGCCGGCG 1 cut(s) 20
MroNI GCCGGC 1 cut(s) 20
MseI TTAA 1 cut(s) 129
MslI CAYNNNNRTG 1 cut(s) 110
MspI CCGG 5 cut(s) 21, 55, 69, 299, 390
MspR9I CCNGG 3 cut(s) 55, 56, 70
MwoI GCNNNNNNNGC 1 cut(s) 189
NaeI GCCGGC 1 cut(s) 22
NciI CCSGG 3 cut(s) 55, 56, 70
NcoI CCATGG 1 cut(s) 162
NdeII GATC 4 cut(s) 151, 184, 205, 534
NgoMIV GCCGGC 1 cut(s) 20
NlaIII CATG 1 cut(s) 166
NlaIV GGNNCC 3 cut(s) 38, 53, 67
NmuCI GTSAC 3 cut(s) 171, 392, 429
NsbI TGCGCA 1 cut(s) 408
PaeR7I CTCGAG 1 cut(s) 512
PdiI GCCGGC 1 cut(s) 22
PinAI ACCGGT 1 cut(s) 389
PkrI GCNGC 2 cut(s) 196, 329
PmaCI CACGTG 1 cut(s) 142
PmlI CACGTG 1 cut(s) 142
Ppu21I YACGTR 1 cut(s) 142
PpuMI RGGWCCY 1 cut(s) 51
PshBI ATTAAT 1 cut(s) 129
Psp5II RGGWCCY 1 cut(s) 51
PspCI CACGTG 1 cut(s) 142
PspEI GGTNACC 1 cut(s) 429
PspN4I GGNNCC 3 cut(s) 38, 53, 67
PspPI GGNCC 1 cut(s) 51
PspPPI RGGWCCY 1 cut(s) 51
PspXI VCTCGAGB 1 cut(s) 512
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 1 cut(s) 220
RsaNI GTAC 1 cut(s) 219
RseI CAYNNNNRTG 1 cut(s) 110
SaqAI TTAA 1 cut(s) 129
SatI GCNGC 2 cut(s) 195, 328
Sau3AI GATC 4 cut(s) 151, 184, 205, 534
Sau96I GGNCC 1 cut(s) 51
ScrFI CCNGG 3 cut(s) 55, 56, 70
SduI GDGCHC 3 cut(s) 39, 149, 240
SetI ASST 7 cut(s) 8, 80, 114, 144, 493, 513, 519
Sfr274I CTCGAG 1 cut(s) 512
SgrAI CRCCGGYG 1 cut(s) 20
SinI GGWCC 1 cut(s) 51
SlaI CTCGAG 1 cut(s) 512
SmaI CCCGGG 1 cut(s) 56
SmiMI CAYNNNNRTG 1 cut(s) 110
SmlI CTYRAG 2 cut(s) 209, 512
SmoI CTYRAG 2 cut(s) 209, 512
SsiI CCGC 7 cut(s) 24, 197, 278, 327, 373, 439, 443
SspMI CTAG 2 cut(s) 75, 538
StyD4I CCNGG 3 cut(s) 53, 54, 68
StyI CCWWGG 2 cut(s) 162, 265
TaaI ACNGT 2 cut(s) 321, 398
TaiI ACGT 3 cut(s) 114, 144, 493
TaqI TCGA 3 cut(s) 11, 96, 513
TauI GCSGC 1 cut(s) 330
Tru1I TTAA 1 cut(s) 129
Tru9I TTAA 1 cut(s) 129
TseFI GTSAC 3 cut(s) 171, 392, 429
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 3 cut(s) 171, 392, 429
TspGWI ACGGA 1 cut(s) 164
TspMI CCCGGG 1 cut(s) 54
VpaK11BI GGWCC 1 cut(s) 51
VspI ATTAAT 1 cut(s) 129
XhoI CTCGAG 1 cut(s) 512
XmaI CCCGGG 1 cut(s) 54
XspI CTAG 2 cut(s) 75, 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.