Rorug03G0332000

Potato inhibitor I family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000470
Physical Location & Seq
Forward (+)
38529803 .. 38540973
11171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0332000.1

Sequence Viewer

Length: 2922 bp
ATGGTAGAATTGGGAATGAGCAAGGCCAGCAAACCGGTGAGTGTGAGCTTCAGAATACCATATTACACTCAGTGGGGCCAAAGCCTGGTGGTGTGCGGCTCCGAACCGGTGCTTGGTTCTTGGAATGTCAAGAGGGGTCTGCTGTTGAGTCCCGTTCATCAGGGTGATGAGCTCATATGGTTCGGCACCATTTCTGTCCCCAAAGGGTTTGGATCGTGCGAGTACAGTTACTACGTTGTGGATGCTGATGATCGAAGTGTTCTTCGATGGGAGATGGGAAACAAGCGTCGGCTTCTTTTGCCTCAGGCCATTCAGGAAGGAGAGCTGCTTCACTTCCATGACTTTTGGCAGGTTGGTGCCGATGCACTTCCTTTCAGAAGTGCCTTCAAGGACGTCATCTTTCGTAGAGAGTGGAGTTTGGAGATTGAGAAACCTCTAGGAGTCATCCAAAATTCATTGCAGAATGATGATTCTGTTCTCGTCCATTTCAAAATTTGCTGTCCAAATATAGAAGAAGGAACATCGATTTACATTATTGGTAGTACCTCAAAGTTGGGGAACTGGAAGGCACAGGATGGGCTTAAACTAAGCTATTCTGGTGAATCAACTTGGCATGCAGACTGTGTCGTGCCGAAAGGGGACTTCCCGATAAAATATAAGTACTGTAAATATAGCAAAGGAGGAAACATTTCTTCTGAAACTGGTCCAAACTGCGAAATTGCTCTTGACTCGTTAAGTACTGAACCAAGATACCTTTTCCGTTCAGATGGCATGTTGCGAGAAATGCCTTGGCGGGGGGCTGGTGTTGCAATCCCAATGTTCTCTGTTAGATCAGAAACTGATCTTGGTGTTGGAGAGTTTCTTGACCTGAAATTACTTGTTGACTGGGCTGCAGAGTCTGGCTTTCATTTAGTTCAGCTTTTACCCATTAATGATACATCTGTGTATGGAATGTGGTGGGATTCATATCCTTACAGCTCACTTTCTGTATATGCATTGCATCCTTTATACTTGAGAGTACAGGCACTTTCAGAAAACATCCCCAAGGATATCAAGGTAGAGATTCAGAAGGCAAAAGAAGAATTGGAGGGAAAGGATGTTGATTACGAGGCTACCTTGACCACTAAACTCTCAATTGCAAAGAAAATATTTGCCCAAGAGAAGAATAAGATTCTCAGCTCCAATTCCTTCCAGAAATTTTTCTCTGAGAATCAGGAATGGTTAAAACCCTATGCGGCCTTCTGTTTTTTGCGAGACTTCTTTGAAACATCAGATCACAGTCAATGGGGTCGGTTTTCCCAGTTTTCAATAGAAAAGCTAGAGAAACTCATCTCTAAAGACAGCATTCACTATCGTGAAATTTGCTTCCATTATTACATCCAATTTCATTTGCATATGCAGCTGTCGGAAGCTGCAGAATATGCAAGAAAGAAGGGAGTAATATTGAAAGGAGACTTGCCTATTGGTGTTGGTAGAGACAGTGTAGATACATGGGTCTATCCAAATCTGTTTCGCATGAACACATCTACTGGAGCTCCTCCAGATTATTTTGATAAAAATGGGCAGAACTGGGGTTTCCCCACTTACAATTGGGACGAGATGGCCAAAGACAATTATGCCTGGTGGCGTGCTCGATTGACACAGATGGCAAAATACTTTACAGCTTACCGGATTGACCATATATTGGGGTTCTTTCGTATCTGGGAACTCCCAGAGCATGCCATCACTGGTCTCGTTGGAAAATTTCGTCCTTCCATTCCTCTAAGTCAGGAAGAACTTGAAAAAGAGGGCATATGGGACTTTGATCGCTTAGCTCGTCCATATATCCCACAGGAAATTTTACAGATTAAGTTTGGAGATTCATGGACTTTTATTGCTTCGAGTTTTCTGAATGAATATCAAAAGAACCACTATGAGTTTAAGGAGGACTGCAATACAGAGAAAAAAATTGCTTCCAAGCTGAAGTCATTTTCAGGAAGATCGTTGTTGCAGAATGAAGACCACATTAGGCATGAGCTCTTTGATATTTTACAGAATATAGTTCTGATAAGAGATCCAGAAAATCCAAGGAACTTCTATCCTCGCTTCAATCTCGAAGAGACTTCAAGTTTTAAGGATTTGGATGATCACTGCAAGAATGTACTGAAACGGTTATACTATGATTACTACTTCCATCGACAAGAAAATCTTTGGCGTCAAAATGCGTTGAAGACACTGCCTGCTCTGCTGAACTCATCGGATATGCTAGCTTGTGGGGAAGATCTGGGGCTTATTCCTTCTTGTGTTTATCCTGTGATGCAAGAACTGGGATTAATAGGTTTACGCATTCAACGTATGCCTAGTGAACCTGATCTGGAGTTTGGTATCCCTTCTCGATACAGTTATATGACAGTGTGTGCTCCATCATGCCATGACTGTTCAACATTGCGTGCTTGGTGGGAAGAAGATGAAGAAAGGAGGCAGCGATTTTTTAACAGTATGGTGGGATCTGACTTGTTGCCACCCAGTCGCTGTGTTCCAGAGATTGCAAATTTCATCATAAGGCAACATTTTGAAGCTCCATCAATGTGGGCAATTTTCCCTCTTCAGGATTTGTTGATTTTGAAAGAAGAATACACAACACGCCCTGCAAAAGAGGAGACAATCAATGACCCCACAAATCCGAAGCATTATTGGAGATACCGTGTACATGTGACATTGGAGGCTTTGATCAAGGATAAAGAGCTCACATCGATCATCAAAGACCTCGTGCGTGGAAGTGGAAGATCACATCCGGGGAAACAAGCTGAGAAACAAGTGATTGAGGAATCATCTGTTGCAACCACAGAGAAGAAGCAGATTGCCAGCAGCAAAGAAAAGGCTCAAGTGGCAACTCCACTGAATGGAGTACCGCGGAAGGAGGCTGTTGCTGTTCCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
potato_inhibit

Protein Analysis

973

Amino Acids

112.42

Weight (kDa)

6.01

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_20 PF00686 13 - 105 1.8e-20 Starch binding domain
CBM_20 PF00686 159 - 242 3.1e-14 Starch binding domain
Glyco_hydro_77 PF02446 271 - 898 5.1e-146 4-alpha-glucanotransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43570 AT5G43570 AT5G43580
fragaria_vesca FvH4_4g02840 FvH4_4g02850 FvH4_4g02860 FvH4_4g02870 FvH4_4g02880 FvH4_4g02930
malus_domestica MD13G1208300.v1.1 MD13G1208400.v1.1 MD13G1208500.v1.1 MD13G1208700.v1.1 MD16G1210300.v1.1 MD16G1210400.v1.1
prunus_persica Prupe.1G032000_v2.0.a1 Prupe.1G032100_v2.0.a1 Prupe.1G032400_v2.0.a1 Prupe.1G032500_v2.0.a1 Prupe.1G032700_v2.0.a1 Prupe.I000200_v2.0.a1 Prupe.I000300_v2.0.a1
pyrus_communis pycom13g18030 pycom13g18040 pycom13g18050 pycom13g18060 pycom13g18080
rosa_chinensis RchiOBHm_Chr4g0390821 RchiOBHm_Chr4g0390831 RchiOBHm_Chr4g0390841 RchiOBHm_Chr4g0390891 RchiOBHm_Chr4g0390911 RchiOBHm_Chr4g0390921 RchiOBHm_Chr4g0390951 RchiOBHm_Chr4g0390971
rosa_laevigata RLG00000009909
rosa_multiflora Rmu_co8497379.1_g000001 Rmu_sc0000171.1_g000002 Rmu_sc0000171.1_g000003 Rmu_sc0000171.1_g000004 Rmu_sc0000171.1_g000009 Rmu_sc0000171.1_g000010 Rmu_sc0000171.1_g000014 Rmu_sc0000171.1_g000018 Rmu_sc0000171.1_g000019 Rmu_sc0001824.1_g000004 Rmu_sc0016732.1_g000001
rosa_roxburghii Rroxscaffold_5G00336740 Rroxscaffold_5G00336750 Rroxscaffold_5G00336760 Rroxscaffold_5G00336770 Rroxscaffold_5G00336790 Rroxscaffold_5G00336830
rosa_rugosa Rorug03G0331900 Rorug03G0331900 Rorug03G0331900 Rorug03G0332000 Rorug03G0332300 Rorug03G0332500
rosa_samantha Rh4AG033300 Rh4AG033400 Rh4AG033500 Rh4AG033600 Rh4AG033800 Rh4AG034000 Rh4BG027200 Rh4BG027300 Rh4BG027500 Rh4BG027700 Rh4BG027800 Rh4BG028100 Rh4BG028200 Rh4BG028400 Rh4CG036900 Rh4CG037000 Rh4CG037200 Rh4CG037400 Rh4DG030300 Rh4DG030400 Rh4DG030500 Rh4DG030600 Rh4DG030800 Rh4DG031100 Rh4DG031300
rosa_wichuraiana Rw4G002560 Rw4G002580 Rw4G002600 Rw4G002610 Rw4G002620 Rw4G002640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 396
Acc36I ACCTGC 1 cut(s) 340
AccB1I GGYRCC 2 cut(s) 185, 356
AccB7I CCANNNNNTGG 3 cut(s) 85, 1684, 2882
AccII CGCG 1 cut(s) 2893
AciI CCGC 5 cut(s) 96, 793, 1235, 2891, 2893
AclWI GGATC 3 cut(s) 220, 2048, 2494
AcoI YGGCCR 1 cut(s) 1602
AcsI RAATTY 7 cut(s) 451, 492, 1196, 1359, 1742, 1836, 2530
AcuI CTGAAG 3 cut(s) 34, 1982, 2570
AcyI GRCGYC 2 cut(s) 393, 2194
AdeI CACNNNGTG 1 cut(s) 72
AfaI GTAC 8 cut(s) 224, 544, 662, 739, 1020, 2142, 2688, 2889
AfiI CCNNNNNNNGG 6 cut(s) 85, 113, 794, 1684, 2587, 2882
AflIII ACRYGT 1 cut(s) 2689
AgeI ACCGGT 2 cut(s) 34, 106
AjnI CCWGG 2 cut(s) 84, 1619
AjuI GAANNNNNNNTTGG 4 cut(s) 96, 128, 828, 860
AleI CACNNNNGTG 1 cut(s) 1353
Alw21I GWGCWC 6 cut(s) 174, 1537, 1633, 2019, 2401, 2727
Alw26I GTCTC 6 cut(s) 1248, 1446, 1470, 1736, 2093, 2633
AlwI GGATC 3 cut(s) 220, 2048, 2494
AlwNI CAGNNNCTG 3 cut(s) 839, 899, 2511
AoxI GGCC 5 cut(s) 24, 76, 306, 1236, 1602
ApeKI GCWGC 6 cut(s) 325, 890, 1399, 1412, 2461, 2847
ApoI RAATTY 7 cut(s) 451, 492, 1196, 1359, 1742, 1836, 2530
AseI ATTAAT 2 cut(s) 930, 2312
AsiGI ACCGGT 2 cut(s) 34, 106
Asp700I GAANNNNTTC 2 cut(s) 688, 1199
AspS9I GGNCC 2 cut(s) 76, 704
AsuC2I CCSGG 1 cut(s) 2775
AsuHPI GGTGA 3 cut(s) 49, 176, 611
AsuNHI GCTAGC 1 cut(s) 2245
AvaII GGWCC 1 cut(s) 704
AxyI CCTNAGG 1 cut(s) 303
BaeI ACNNNNGTAYC 4 cut(s) 927, 960, 1479, 1512
BalI TGGCCA 1 cut(s) 1604
BanI GGYRCC 2 cut(s) 185, 356
BanII GRGCYC 4 cut(s) 174, 1537, 2019, 2727
BauI CACGAG 1 cut(s) 2747
BbsI GAAGAC 2 cut(s) 2004, 2216
Bbv12I GWGCWC 6 cut(s) 174, 1537, 1633, 2019, 2401, 2727
BbvI GCAGC 6 cut(s) 312, 877, 1399, 1411, 2473, 2859
BciT130I CCWGG 2 cut(s) 86, 1621
BciVI GTATCC 1 cut(s) 2375
BclI TGATCA 2 cut(s) 2125, 2709
BcnI CCSGG 1 cut(s) 2775
BcoDI GTCTC 6 cut(s) 1248, 1446, 1470, 1736, 2093, 2633
BfaI CTAG 4 cut(s) 437, 1319, 2246, 2340
BfmI CTRYAG 2 cut(s) 891, 1413
BfuAI ACCTGC 1 cut(s) 340
BfuI GTATCC 1 cut(s) 2375
BglII AGATCT 1 cut(s) 2260
BisI GCNGC 8 cut(s) 97, 326, 891, 1236, 1400, 1413, 2462, 2848
BlpI GCTNAGC 1 cut(s) 1810
BlsI GCNGC 8 cut(s) 98, 327, 892, 1237, 1401, 1414, 2463, 2849
BmcAI AGTACT 2 cut(s) 662, 739
Bme1390I CCNGG 3 cut(s) 86, 1621, 2775
Bme18I GGWCC 1 cut(s) 704
BmgT120I GGNCC 2 cut(s) 76, 704
BmiI GGNNCC 4 cut(s) 77, 100, 187, 358
BmrFI CCNGG 3 cut(s) 86, 1621, 2775
BmrI ACTGGG 5 cut(s) 895, 1294, 1579, 2315, 2499
BmsI GCATC 4 cut(s) 232, 352, 1009, 2286
BmtI GCTAGC 1 cut(s) 2249
BmuI ACTGGG 5 cut(s) 895, 1294, 1579, 2315, 2499
BpiI GAAGAC 2 cut(s) 2004, 2216
BpmI CTGGAG 3 cut(s) 1524, 1551, 2375
Bpu1102I GCTNAGC 1 cut(s) 1810
BpuEI CTTGAG 2 cut(s) 1033, 2847
BpuMI CCSGG 1 cut(s) 2775
Bsa29I ATCGAT 2 cut(s) 524, 2732
BsaBI GATNNNNATC 1 cut(s) 966
BsaHI GRCGYC 2 cut(s) 393, 2194
BsaI GGTCTC 1 cut(s) 1736
BsaJI CCNNGG 5 cut(s) 788, 1044, 2066, 2774, 2891
BsaWI WCCGGW 3 cut(s) 34, 106, 1668
BsaXI ACNNNNNCTCC 4 cut(s) 1519, 1549, 2668, 2698
Bsc4I CCNNNNNNNGG 6 cut(s) 85, 113, 794, 1684, 2587, 2882
Bse118I RCCGGY 2 cut(s) 34, 106
Bse1I ACTGG 9 cut(s) 566, 706, 890, 1300, 1534, 1574, 1732, 2310, 2505
Bse21I CCTNAGG 1 cut(s) 303
Bse3DI GCAATG 3 cut(s) 455, 995, 2423
Bse8I GATNNNNATC 1 cut(s) 966
BseBI CCWGG 2 cut(s) 86, 1621
BseCI ATCGAT 2 cut(s) 524, 2732
BseDI CCNNGG 5 cut(s) 788, 1044, 2066, 2774, 2891
BseGI GGATG 9 cut(s) 247, 444, 580, 1000, 1038, 1102, 1377, 2128, 2770
BseJI GATNNNNATC 1 cut(s) 966
BseLI CCNNNNNNNGG 6 cut(s) 85, 113, 794, 1684, 2587, 2882
BseMI GCAATG 3 cut(s) 455, 995, 2423
BseMII CTCAG 5 cut(s) 83, 317, 1189, 1197, 2778
BseNI ACTGG 9 cut(s) 566, 706, 890, 1300, 1534, 1574, 1732, 2310, 2505
BseRI GAGGAG 2 cut(s) 1527, 2651
BseXI GCAGC 6 cut(s) 312, 877, 1399, 1411, 2473, 2859
Bsh1236I CGCG 1 cut(s) 2893
BshFI GGCC 5 cut(s) 26, 78, 308, 1238, 1604
BshNI GGYRCC 2 cut(s) 185, 356
BshTI ACCGGT 2 cut(s) 34, 106
BshVI ATCGAT 2 cut(s) 524, 2732
BsiHKAI GWGCWC 6 cut(s) 174, 1537, 1633, 2019, 2401, 2727
BsiSI CCGG 4 cut(s) 35, 107, 1669, 2774
BslFI GGGAC 5 cut(s) 135, 182, 653, 1607, 1811
BslI CCNNNNNNNGG 6 cut(s) 85, 113, 794, 1684, 2587, 2882
BsmAI GTCTC 6 cut(s) 1248, 1446, 1470, 1736, 2093, 2633
BsmFI GGGAC 5 cut(s) 135, 182, 653, 1607, 1811
BsmI GAATGC 2 cut(s) 1344, 2325
BsnI GGCC 5 cut(s) 26, 78, 308, 1238, 1604
Bso31I GGTCTC 1 cut(s) 1736
Bsp1286I GDGCHC 6 cut(s) 174, 1537, 1633, 2019, 2401, 2727
Bsp1407I TGTACA 1 cut(s) 2686
Bsp1720I GCTNAGC 1 cut(s) 1810
BspACI CCGC 5 cut(s) 96, 793, 1235, 2891, 2893
BspANI GGCC 5 cut(s) 26, 78, 308, 1238, 1604
BspCNI CTCAG 5 cut(s) 82, 316, 1188, 1198, 2779
BspDI ATCGAT 2 cut(s) 524, 2732
BspFNI CGCG 1 cut(s) 2893
BspLI GGNNCC 4 cut(s) 77, 100, 187, 358
BspMAI CTGCAG 2 cut(s) 895, 1417
BspMI ACCTGC 1 cut(s) 340
BspOI GCTAGC 1 cut(s) 2249
BspPI GGATC 3 cut(s) 220, 2048, 2494
BspT107I GGYRCC 2 cut(s) 185, 356
BspTNI GGTCTC 1 cut(s) 1736
BsrDI GCAATG 3 cut(s) 455, 995, 2423
BsrFI RCCGGY 2 cut(s) 34, 106
BsrGI TGTACA 1 cut(s) 2686
BsrI ACTGG 9 cut(s) 566, 706, 890, 1300, 1534, 1574, 1732, 2310, 2505
BssAI RCCGGY 2 cut(s) 34, 106
BssECI CCNNGG 5 cut(s) 788, 1044, 2066, 2774, 2891
BssNI GRCGYC 2 cut(s) 393, 2194
BssSI CACGAG 1 cut(s) 2747
BssT1I CCWWGG 3 cut(s) 788, 1044, 2066
Bst2BI CACGAG 1 cut(s) 2747
Bst2UI CCWGG 2 cut(s) 86, 1621
Bst6I CTCTTC 2 cut(s) 2091, 2589
BstACI GRCGYC 2 cut(s) 393, 2194
BstAPI GCANNNNNTGC 1 cut(s) 1421
BstAUI TGTACA 1 cut(s) 2686
BstC8I GCNNGC 8 cut(s) 28, 615, 1629, 1719, 2220, 2247, 2430, 2845
BstDEI CTNAG 8 cut(s) 69, 303, 587, 1175, 1206, 1763, 1810, 2787
BstDSI CCRYGG 1 cut(s) 2891
BstF5I GGATG 9 cut(s) 247, 444, 580, 1000, 1038, 1102, 1377, 2128, 2770
BstFNI CGCG 1 cut(s) 2893
BstMAI GTCTC 6 cut(s) 1248, 1446, 1470, 1736, 2093, 2633
BstMWI GCNNNNNNNGC 8 cut(s) 27, 298, 784, 806, 1399, 1421, 2224, 2867
BstNI CCWGG 2 cut(s) 86, 1621
BstNSI RCATGY 4 cut(s) 617, 775, 1721, 2693
BstSCI CCNGG 3 cut(s) 84, 1619, 2773
BstSFI CTRYAG 2 cut(s) 891, 1413
BstUI CGCG 1 cut(s) 2893
BstV1I GCAGC 6 cut(s) 312, 877, 1399, 1411, 2473, 2859
BstV2I GAAGAC 2 cut(s) 2004, 2216
BstX2I RGATCY 3 cut(s) 2053, 2260, 2486
BstXI CCANNNNNNTGG 1 cut(s) 2568
BstYI RGATCY 3 cut(s) 2053, 2260, 2486
Bsu15I ATCGAT 2 cut(s) 524, 2732
Bsu36I CCTNAGG 1 cut(s) 303
BsuI GTATCC 1 cut(s) 2375
BsuRI GGCC 5 cut(s) 26, 78, 308, 1238, 1604
BsuTUI ATCGAT 2 cut(s) 524, 2732
BtgI CCRYGG 1 cut(s) 2891
BtsCI GGATG 9 cut(s) 247, 444, 580, 1000, 1038, 1102, 1377, 2128, 2770
BtsI GCAGTG 2 cut(s) 2128, 2213
BtsIMutI CAGTG 7 cut(s) 77, 1486, 1725, 2128, 2213, 2397, 2876
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 8 cut(s) 28, 615, 1629, 1719, 2220, 2247, 2430, 2845
CaiI CAGNNNCTG 3 cut(s) 839, 899, 2511
Cfr10I RCCGGY 2 cut(s) 34, 106
Cfr13I GGNCC 2 cut(s) 76, 704
Cfr42I CCGCGG 1 cut(s) 2894
ClaI ATCGAT 2 cut(s) 524, 2732
CseI GACGC 2 cut(s) 275, 2183
Csp6I GTAC 8 cut(s) 223, 543, 661, 738, 1019, 2141, 2687, 2888
CspAI ACCGGT 2 cut(s) 34, 106
CviQI GTAC 8 cut(s) 223, 543, 661, 738, 1019, 2141, 2687, 2888
DdeI CTNAG 8 cut(s) 69, 303, 587, 1175, 1206, 1763, 1810, 2787
DraIII CACNNNGTG 1 cut(s) 72
EaeI YGGCCR 1 cut(s) 1602
Eam1104I CTCTTC 2 cut(s) 2091, 2589
EarI CTCTTC 2 cut(s) 2091, 2589
Ecl136II GAGCTC 4 cut(s) 172, 1535, 2017, 2725
Eco130I CCWWGG 3 cut(s) 788, 1044, 2066
Eco24I GRGCYC 4 cut(s) 174, 1537, 2019, 2727
Eco31I GGTCTC 1 cut(s) 1736
Eco32I GATATC 1 cut(s) 1051
Eco47I GGWCC 1 cut(s) 704
Eco53kI GAGCTC 4 cut(s) 172, 1535, 2017, 2725
Eco57I CTGAAG 3 cut(s) 34, 1982, 2570
Eco81I CCTNAGG 1 cut(s) 303
EcoICRI GAGCTC 4 cut(s) 172, 1535, 2017, 2725
EcoRII CCWGG 2 cut(s) 84, 1619
EcoRV GATATC 1 cut(s) 1051
EcoT14I CCWWGG 3 cut(s) 788, 1044, 2066
EcoT22I ATGCAT 1 cut(s) 997
EcoT38I GRGCYC 4 cut(s) 174, 1537, 2019, 2727
ErhI CCWWGG 3 cut(s) 788, 1044, 2066
FalI AAGNNNNNCTT 2 cut(s) 2172, 2204
FaqI GGGAC 5 cut(s) 135, 182, 653, 1607, 1811
FauI CCCGC 1 cut(s) 786
FauNDI CATATG 3 cut(s) 176, 1395, 1793
FbaI TGATCA 2 cut(s) 2125, 2709
Fnu4HI GCNGC 8 cut(s) 97, 326, 891, 1236, 1400, 1413, 2462, 2848
FokI GGATG 9 cut(s) 254, 431, 587, 987, 1025, 1109, 1364, 2135, 2757
FriOI GRGCYC 4 cut(s) 174, 1537, 2019, 2727
Fsp4HI GCNGC 8 cut(s) 97, 326, 891, 1236, 1400, 1413, 2462, 2848
FspBI CTAG 4 cut(s) 437, 1319, 2246, 2340
GluI GCNGC 8 cut(s) 97, 326, 891, 1236, 1400, 1413, 2462, 2848
GsuI CTGGAG 3 cut(s) 1524, 1551, 2375
HaeIII GGCC 5 cut(s) 26, 78, 308, 1238, 1604
HapII CCGG 4 cut(s) 35, 107, 1669, 2774
HgaI GACGC 2 cut(s) 275, 2183
Hin1I GRCGYC 2 cut(s) 393, 2194
HincII GTYRAC 1 cut(s) 883
HindII GTYRAC 1 cut(s) 883
HpaII CCGG 4 cut(s) 35, 107, 1669, 2774
HphI GGTGA 3 cut(s) 49, 176, 611
Hpy166II GTNNAC 4 cut(s) 883, 2321, 2345, 2687
Hpy8I GTNNAC 4 cut(s) 883, 2321, 2345, 2687
Hpy99I CGWCG 1 cut(s) 291
HpyCH4IV ACGT 3 cut(s) 234, 393, 2332
HpyF10VI GCNNNNNNNGC 8 cut(s) 27, 298, 784, 806, 1399, 1421, 2224, 2867
HpyF3I CTNAG 8 cut(s) 69, 303, 587, 1175, 1206, 1763, 1810, 2787
HpySE526I ACGT 3 cut(s) 234, 393, 2332
Hsp92I GRCGYC 2 cut(s) 393, 2194
Ksp22I TGATCA 2 cut(s) 2125, 2709
KspI CCGCGG 1 cut(s) 2894
LmnI GCTCC 6 cut(s) 104, 1184, 1532, 1540, 2404, 2563
Lsp1109I GCAGC 6 cut(s) 312, 877, 1399, 1411, 2473, 2859
LweI GCATC 4 cut(s) 232, 352, 1009, 2286
MaeI CTAG 4 cut(s) 437, 1319, 2246, 2340
MaeII ACGT 3 cut(s) 234, 393, 2332
MaeIII GTNAC 2 cut(s) 227, 2692
MfeI CAATTG 2 cut(s) 1134, 1588
MflI RGATCY 3 cut(s) 2053, 2260, 2486
MhlI GDGCHC 6 cut(s) 174, 1537, 1633, 2019, 2401, 2727
MlsI TGGCCA 1 cut(s) 1604
MluNI TGGCCA 1 cut(s) 1604
MlyI GAGTC 4 cut(s) 157, 450, 722, 905
MmeI TCCRAC 3 cut(s) 832, 1386, 1717
Mox20I TGGCCA 1 cut(s) 1604
Mph1103I ATGCAT 1 cut(s) 997
MroXI GAANNNNTTC 2 cut(s) 688, 1199
MscI TGGCCA 1 cut(s) 1604
MslI CAYNNNNRTG 4 cut(s) 162, 336, 1353, 2566
Msp20I TGGCCA 1 cut(s) 1604
MspA1I CMGCKG 2 cut(s) 1402, 2893
MspI CCGG 4 cut(s) 35, 107, 1669, 2774
MspR9I CCNGG 3 cut(s) 86, 1621, 2775
MunI CAATTG 2 cut(s) 1134, 1588
Mva1269I GAATGC 2 cut(s) 1344, 2325
MvaI CCWGG 2 cut(s) 86, 1621
MvnI CGCG 1 cut(s) 2893
MwoI GCNNNNNNNGC 8 cut(s) 27, 298, 784, 806, 1399, 1421, 2224, 2867
NciI CCSGG 1 cut(s) 2775
NdeI CATATG 3 cut(s) 176, 1395, 1793
NheI GCTAGC 1 cut(s) 2245
NlaIV GGNNCC 4 cut(s) 77, 100, 187, 358
NmuCI GTSAC 1 cut(s) 2692
NsiI ATGCAT 1 cut(s) 997
NspI RCATGY 4 cut(s) 617, 775, 1721, 2693
OliI CACNNNNGTG 1 cut(s) 1353
PaeI GCATGC 2 cut(s) 617, 1721
PciI ACATGT 1 cut(s) 2689
PcsI WCGNNNNNNNCGW 2 cut(s) 1813, 2329
PctI GAATGC 2 cut(s) 1344, 2325
PdmI GAANNNNTTC 2 cut(s) 688, 1199
PfeI GAWTC 8 cut(s) 470, 602, 962, 1063, 1171, 1210, 1859, 2807
PflFI GACNNNGTC 1 cut(s) 623
PflMI CCANNNNNTGG 3 cut(s) 85, 1684, 2882
PinAI ACCGGT 2 cut(s) 34, 106
PkrI GCNGC 8 cut(s) 98, 327, 892, 1237, 1401, 1414, 2463, 2849
PleI GAGTC 4 cut(s) 156, 449, 722, 904
PpsI GAGTC 4 cut(s) 156, 449, 722, 904
PscI ACATGT 1 cut(s) 2689
PshBI ATTAAT 2 cut(s) 930, 2312
Psp124BI GAGCTC 4 cut(s) 174, 1537, 2019, 2727
Psp6I CCWGG 2 cut(s) 84, 1619
PspGI CCWGG 2 cut(s) 84, 1619
PspN4I GGNNCC 4 cut(s) 77, 100, 187, 358
PspPI GGNCC 2 cut(s) 76, 704
PstI CTGCAG 2 cut(s) 895, 1417
PstNI CAGNNNCTG 3 cut(s) 839, 899, 2511
PsuI RGATCY 3 cut(s) 2053, 2260, 2486
PsyI GACNNNGTC 1 cut(s) 623
PvuII CAGCTG 1 cut(s) 1402
RsaI GTAC 8 cut(s) 224, 544, 662, 739, 1020, 2142, 2688, 2889
RsaNI GTAC 8 cut(s) 223, 543, 661, 738, 1019, 2141, 2687, 2888
RseI CAYNNNNRTG 4 cut(s) 162, 336, 1353, 2566
SacI GAGCTC 4 cut(s) 174, 1537, 2019, 2727
SacII CCGCGG 1 cut(s) 2894
SatI GCNGC 8 cut(s) 97, 326, 891, 1236, 1400, 1413, 2462, 2848
Sau96I GGNCC 2 cut(s) 76, 704
ScaI AGTACT 2 cut(s) 662, 739
SchI GAGTC 4 cut(s) 157, 450, 722, 905
ScrFI CCNGG 3 cut(s) 86, 1621, 2775
SduI GDGCHC 6 cut(s) 174, 1537, 1633, 2019, 2401, 2727
SfaNI GCATC 4 cut(s) 232, 352, 1009, 2286
SfcI CTRYAG 2 cut(s) 891, 1413
Sfr303I CCGCGG 1 cut(s) 2894
SgrBI CCGCGG 1 cut(s) 2894
SinI GGWCC 1 cut(s) 704
SmiMI CAYNNNNRTG 4 cut(s) 162, 336, 1353, 2566
SmlI CTYRAG 2 cut(s) 1012, 2862
SmoI CTYRAG 2 cut(s) 1012, 2862
SphI GCATGC 2 cut(s) 617, 1721
SsiI CCGC 5 cut(s) 96, 793, 1235, 2891, 2893
SspI AATATT 2 cut(s) 1149, 1443
SspMI CTAG 4 cut(s) 437, 1319, 2246, 2340
SstI GAGCTC 4 cut(s) 174, 1537, 2019, 2727
StyD4I CCNGG 3 cut(s) 84, 1619, 2773
StyI CCWWGG 3 cut(s) 788, 1044, 2066
TaiI ACGT 3 cut(s) 237, 396, 2335
TaqI TCGA 9 cut(s) 253, 265, 524, 1633, 1880, 2094, 2176, 2374, 2732
TatI WGTACW 6 cut(s) 222, 660, 737, 1018, 2140, 2686
TauI GCSGC 2 cut(s) 99, 1238
TfiI GAWTC 8 cut(s) 470, 602, 962, 1063, 1171, 1210, 1859, 2807
TscAI CASTG 7 cut(s) 77, 1486, 1732, 2135, 2220, 2397, 2883
TseFI GTSAC 1 cut(s) 2692
TseI GCWGC 6 cut(s) 325, 890, 1399, 1412, 2461, 2847
Tsp45I GTSAC 1 cut(s) 2692
TspGWI ACGGA 1 cut(s) 749
TspRI CASTG 7 cut(s) 77, 1486, 1732, 2135, 2220, 2397, 2883
Tth111I GACNNNGTC 1 cut(s) 623
Van91I CCANNNNNTGG 3 cut(s) 85, 1684, 2882
VpaK11BI GGWCC 1 cut(s) 704
VspI ATTAAT 2 cut(s) 930, 2312
XapI RAATTY 7 cut(s) 451, 492, 1196, 1359, 1742, 1836, 2530
XceI RCATGY 4 cut(s) 617, 775, 1721, 2693
XmnI GAANNNNTTC 2 cut(s) 688, 1199
XspI CTAG 4 cut(s) 437, 1319, 2246, 2340
ZraI GACGTC 1 cut(s) 394
ZrmI AGTACT 2 cut(s) 662, 739
Zsp2I ATGCAT 1 cut(s) 997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.