Rh4BG027300

Potato inhibitor I family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000470
Physical Location & Seq
Forward (+)
4958048 .. 4958946
899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG027300.1

Sequence Viewer

Length: 219 bp
ATGGCAGATCAATGCGAAGGTAAGGATTCATGGCCTGAACTGTTGGGAGCTCAGGGGACAGTTGCAAAGGAAACAATTGAGAGCGAAAACTCTTTAGTCAAGGCAGAGATCGTGCTAGAAGGAACAATTGTCCCTATTAATATCCGGAGACAGTGTGATAGGGTTCGTGTTTGGGTCGATACAGATGGCATTGTTACCAGGGTCCCTGTCATTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
potato_inhibit

Protein Analysis

72

Amino Acids

7.91

Weight (kDa)

4.61

Isoelectric Point (pI)

37.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
potato_inhibit PF00280 8 - 72 1.6e-21 Potato inhibitor I family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43570 AT5G43570 AT5G43580
fragaria_vesca FvH4_4g02840 FvH4_4g02850 FvH4_4g02860 FvH4_4g02870 FvH4_4g02880 FvH4_4g02930
malus_domestica MD13G1208300.v1.1 MD13G1208400.v1.1 MD13G1208500.v1.1 MD13G1208700.v1.1 MD16G1210300.v1.1 MD16G1210400.v1.1
prunus_persica Prupe.1G032000_v2.0.a1 Prupe.1G032100_v2.0.a1 Prupe.1G032400_v2.0.a1 Prupe.1G032500_v2.0.a1 Prupe.1G032700_v2.0.a1 Prupe.I000200_v2.0.a1 Prupe.I000300_v2.0.a1
pyrus_communis pycom13g18030 pycom13g18040 pycom13g18050 pycom13g18060 pycom13g18080
rosa_chinensis RchiOBHm_Chr4g0390821 RchiOBHm_Chr4g0390831 RchiOBHm_Chr4g0390841 RchiOBHm_Chr4g0390891 RchiOBHm_Chr4g0390911 RchiOBHm_Chr4g0390921 RchiOBHm_Chr4g0390951 RchiOBHm_Chr4g0390971
rosa_laevigata RLG00000009909
rosa_multiflora Rmu_co8497379.1_g000001 Rmu_sc0000171.1_g000002 Rmu_sc0000171.1_g000003 Rmu_sc0000171.1_g000004 Rmu_sc0000171.1_g000009 Rmu_sc0000171.1_g000010 Rmu_sc0000171.1_g000014 Rmu_sc0000171.1_g000018 Rmu_sc0000171.1_g000019 Rmu_sc0001824.1_g000004 Rmu_sc0016732.1_g000001
rosa_roxburghii Rroxscaffold_5G00336740 Rroxscaffold_5G00336750 Rroxscaffold_5G00336760 Rroxscaffold_5G00336770 Rroxscaffold_5G00336790 Rroxscaffold_5G00336830
rosa_rugosa Rorug03G0331900 Rorug03G0331900 Rorug03G0331900 Rorug03G0332000 Rorug03G0332300 Rorug03G0332500
rosa_samantha Rh4AG033300 Rh4AG033400 Rh4AG033500 Rh4AG033600 Rh4AG033800 Rh4AG034000 Rh4BG027200 Rh4BG027300 Rh4BG027500 Rh4BG027700 Rh4BG027800 Rh4BG028100 Rh4BG028200 Rh4BG028400 Rh4CG036900 Rh4CG037000 Rh4CG037200 Rh4CG037400 Rh4DG030300 Rh4DG030400 Rh4DG030500 Rh4DG030600 Rh4DG030800 Rh4DG031100 Rh4DG031300
rosa_wichuraiana Rw4G002560 Rw4G002580 Rw4G002600 Rw4G002610 Rw4G002620 Rw4G002640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 144
AfiI CCNNNNNNNGG 1 cut(s) 212
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 1 cut(s) 50
AluI AGCT 1 cut(s) 50
Alw21I GWGCWC 1 cut(s) 52
Alw26I GTCTC 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 144
AoxI GGCC 1 cut(s) 32
AseI ATTAAT 1 cut(s) 138
AspS9I GGNCC 1 cut(s) 202
AvaII GGWCC 1 cut(s) 202
BanII GRGCYC 1 cut(s) 52
Bbv12I GWGCWC 1 cut(s) 52
BccI CCATC 1 cut(s) 179
BciT130I CCWGG 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 142
BfaI CTAG 1 cut(s) 116
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 2 cut(s) 203, 204
BmrFI CCNGG 1 cut(s) 199
Bpu10I CCTNAGC 1 cut(s) 51
BsaJI CCNNGG 1 cut(s) 198
BsaWI WCCGGW 1 cut(s) 144
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bsc4I CCNNNNNNNGG 1 cut(s) 212
BseAI TCCGGA 1 cut(s) 144
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 198
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMII CTCAG 1 cut(s) 65
BshFI GGCC 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 52
BsiSI CCGG 1 cut(s) 145
BslFI GGGAC 3 cut(s) 70, 116, 188
BslI CCNNNNNNNGG 1 cut(s) 212
BsmAI GTCTC 1 cut(s) 142
BsmFI GGGAC 3 cut(s) 70, 116, 188
BsnI GGCC 1 cut(s) 34
Bsp1286I GDGCHC 1 cut(s) 52
Bsp13I TCCGGA 1 cut(s) 144
Bsp143I GATC 2 cut(s) 7, 108
BspANI GGCC 1 cut(s) 34
BspCNI CTCAG 1 cut(s) 64
BspEI TCCGGA 1 cut(s) 144
BspLI GGNNCC 2 cut(s) 203, 204
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 2 cut(s) 7, 108
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 3 cut(s) 42, 61, 153
BstDEI CTNAG 1 cut(s) 51
BstKTI GATC 2 cut(s) 10, 111
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 2 cut(s) 7, 108
BstNI CCWGG 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 197
BsuRI GGCC 1 cut(s) 34
BtsIMutI CAGTG 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 202
CviAII CATG 1 cut(s) 30
CviJI RGCY 2 cut(s) 34, 50
CviKI_1 RGCY 2 cut(s) 34, 50
DdeI CTNAG 1 cut(s) 51
DpnI GATC 2 cut(s) 9, 110
DpnII GATC 2 cut(s) 7, 108
Ecl136II GAGCTC 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 202
Eco53kI GAGCTC 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 50
EcoO109I RGGNCCY 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 197
EcoT38I GRGCYC 1 cut(s) 52
FaeI CATG 1 cut(s) 33
FaiI YATR 1 cut(s) 31
FaqI GGGAC 3 cut(s) 70, 116, 188
FatI CATG 1 cut(s) 29
FriOI GRGCYC 1 cut(s) 52
FspBI CTAG 1 cut(s) 116
HaeIII GGCC 1 cut(s) 34
HapII CCGG 1 cut(s) 145
Hin1II CATG 1 cut(s) 33
HinfI GANTC 1 cut(s) 26
HpaII CCGG 1 cut(s) 145
Hpy188III TCNNGA 1 cut(s) 145
HpyAV CCTTC 2 cut(s) 11, 113
HpyCH4III ACNGT 3 cut(s) 42, 61, 153
HpyCH4V TGCA 1 cut(s) 65
HpyF3I CTNAG 1 cut(s) 51
Hsp92II CATG 1 cut(s) 33
KflI GGGWCCC 1 cut(s) 202
Kpn2I TCCGGA 1 cut(s) 144
Kzo9I GATC 2 cut(s) 7, 108
LmnI GCTCC 1 cut(s) 47
LpnPI CCDG 5 cut(s) 38, 48, 158, 184, 211
MaeI CTAG 1 cut(s) 116
MaeIII GTNAC 1 cut(s) 193
MalI GATC 2 cut(s) 9, 110
MboI GATC 2 cut(s) 7, 108
MfeI CAATTG 2 cut(s) 75, 126
MhlI GDGCHC 1 cut(s) 52
MluCI AATT 2 cut(s) 75, 126
MroI TCCGGA 1 cut(s) 144
MseI TTAA 1 cut(s) 138
MspI CCGG 1 cut(s) 145
MspR9I CCNGG 1 cut(s) 199
MunI CAATTG 2 cut(s) 75, 126
MvaI CCWGG 1 cut(s) 199
NdeII GATC 2 cut(s) 7, 108
NlaIII CATG 1 cut(s) 33
NlaIV GGNNCC 2 cut(s) 203, 204
PfeI GAWTC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 202
PshBI ATTAAT 1 cut(s) 138
Psp124BI GAGCTC 1 cut(s) 52
Psp5II RGGWCCY 1 cut(s) 202
Psp6I CCWGG 1 cut(s) 197
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 2 cut(s) 203, 204
PspPI GGNCC 1 cut(s) 202
PspPPI RGGWCCY 1 cut(s) 202
SacI GAGCTC 1 cut(s) 52
SaqAI TTAA 1 cut(s) 138
Sau3AI GATC 2 cut(s) 7, 108
Sau96I GGNCC 1 cut(s) 202
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 1 cut(s) 52
SetI ASST 2 cut(s) 22, 52
SinI GGWCC 1 cut(s) 202
Sse9I AATT 2 cut(s) 75, 126
SspMI CTAG 1 cut(s) 116
SstI GAGCTC 1 cut(s) 52
StyD4I CCNGG 1 cut(s) 197
TaaI ACNGT 3 cut(s) 42, 61, 153
TaqI TCGA 1 cut(s) 177
TasI AATT 2 cut(s) 75, 126
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 1 cut(s) 158
TspDTI ATGAA 1 cut(s) 18
TspRI CASTG 1 cut(s) 158
VpaK11BI GGWCC 1 cut(s) 202
VspI ATTAAT 1 cut(s) 138
XspI CTAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.