Rh2DG251600

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001099
Physical Location & Seq
Reverse (-)
26006800 .. 26046087
39288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG251600.1

Sequence Viewer

Length: 714 bp
ATGAAGGATGAGACACCTTCTTCGTTAGAAGTACCAGGACTTGATAAGGCCAACACTGACTACAGCTCTCTCCGGCGCATCTTGGTTGGAGTTTCAGTGGTTCTATCCACTCTTAATGATGCGGAGGAGAAACAAATCAGAAACCCAGCAGTGAGGGAGTGGCTTGATGAGCTCAGAGATGCTATCTATGTTGCAGACGACTTGTCGGATGAGATGAAGCAAGAATTCGACTCTGGAAGCAGCAGCACAAGTCAGGAAGCTGTGGATGGGTCTAAAGGAAATCATGGATCAATGAGTGAAGAGAGTCAGCTAATCCAGTTGATGGGTCTGACACTTTTTCATTTACTGTGTTCCTCTGCTCACTGTTATGGTCATCAGAGTCGGGAGATGAGGCATATGTGGACAGCACAGAAGCCTGTGGCTTTGGTTCATCTACCAGACATCTCCCAACCTTCTTTACTTCTTACTGAGAAACCTAGAATTGCTCTTTACAGTTCACTCCTGGCGCCACACATTCCTGATCCTGTTTTGCAGTACATAGAGGCATGGCATATCACTTGCAACCTTATACAGAAGAAGTATGGTTTGGCCTGGCCTCAGTTTGCTGGTTGTTGGATAAAGGTGCAGTTTTTGGTGGCTTGGAGGAGGCATCCTGCAGGCACCTCTAAGATCAACCAACAAGAAGTATCAGGTCCTGCTGAACACAAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

237

Amino Acids

26.6

Weight (kDa)

5.78

Isoelectric Point (pI)

52.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 34 - 83 2.9e-11 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 505, 659
AccB7I CCANNNNNTGG 1 cut(s) 322
AciI CCGC 1 cut(s) 122
AclWI GGATC 2 cut(s) 295, 515
AcsI RAATTY 2 cut(s) 224, 707
AcyI GRCGYC 1 cut(s) 506
AfaI GTAC 2 cut(s) 33, 536
AfiI CCNNNNNNNGG 1 cut(s) 322
AhdI GACNNNNNGTC 1 cut(s) 202
AjnI CCWGG 3 cut(s) 34, 501, 590
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AluBI AGCT 4 cut(s) 66, 172, 260, 310
AluI AGCT 4 cut(s) 66, 172, 260, 310
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 1 cut(s) 5
AlwI GGATC 2 cut(s) 295, 515
AlwNI CAGNNNCTG 1 cut(s) 695
AoxI GGCC 3 cut(s) 48, 588, 593
ApeKI GCWGC 2 cut(s) 240, 243
ApoI RAATTY 2 cut(s) 224, 707
ArsI GACNNNNNNTTYG 1 cut(s) 36
AspLEI GCGC 2 cut(s) 78, 508
AspS9I GGNCC 1 cut(s) 692
AvaII GGWCC 1 cut(s) 692
BanI GGYRCC 2 cut(s) 505, 659
BanII GRGCYC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 2 cut(s) 252, 255
BccI CCATC 2 cut(s) 260, 316
BciT130I CCWGG 3 cut(s) 36, 503, 592
BcoDI GTCTC 1 cut(s) 5
BfaI CTAG 1 cut(s) 477
BfmI CTRYAG 2 cut(s) 61, 654
BfoI RGCGCY 1 cut(s) 509
BisI GCNGC 2 cut(s) 241, 244
BlsI GCNGC 2 cut(s) 242, 245
Bme1390I CCNGG 3 cut(s) 36, 503, 592
Bme18I GGWCC 1 cut(s) 692
BmeRI GACNNNNNGTC 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 692
BmiI GGNNCC 2 cut(s) 507, 661
BmrFI CCNGG 3 cut(s) 36, 503, 592
BmsI GCATC 4 cut(s) 87, 109, 169, 658
BsaHI GRCGYC 1 cut(s) 506
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse1I ACTGG 1 cut(s) 316
BseBI CCWGG 3 cut(s) 36, 503, 592
BseGI GGATG 4 cut(s) 13, 214, 271, 649
BseLI CCNNNNNNNGG 1 cut(s) 322
BseMII CTCAG 3 cut(s) 187, 459, 611
BseNI ACTGG 1 cut(s) 316
BseRI GAGGAG 2 cut(s) 140, 658
BseXI GCAGC 2 cut(s) 252, 255
BseYI CCCAGC 1 cut(s) 145
BsgI GTGCAG 1 cut(s) 644
BshFI GGCC 3 cut(s) 50, 590, 595
BshNI GGYRCC 2 cut(s) 505, 659
BsiHKAI GWGCWC 1 cut(s) 174
BsiSI CCGG 1 cut(s) 73
BslI CCNNNNNNNGG 1 cut(s) 322
BsmAI GTCTC 1 cut(s) 5
BsnI GGCC 3 cut(s) 50, 590, 595
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 3 cut(s) 287, 520, 669
BspACI CCGC 1 cut(s) 122
BspANI GGCC 3 cut(s) 50, 590, 595
BspCNI CTCAG 3 cut(s) 186, 460, 610
BspLI GGNNCC 2 cut(s) 507, 661
BspMAI CTGCAG 1 cut(s) 658
BspPI GGATC 2 cut(s) 295, 515
BspT107I GGYRCC 2 cut(s) 505, 659
BsrI ACTGG 1 cut(s) 316
BssMI GATC 3 cut(s) 287, 520, 669
BssNI GRCGYC 1 cut(s) 506
Bst2UI CCWGG 3 cut(s) 36, 503, 592
Bst4CI ACNGT 3 cut(s) 348, 365, 494
Bst6I CTCTTC 1 cut(s) 294
BstACI GRCGYC 1 cut(s) 506
BstC8I GCNNGC 1 cut(s) 658
BstDEI CTNAG 4 cut(s) 173, 468, 597, 666
BstF5I GGATG 4 cut(s) 13, 214, 271, 649
BstH2I RGCGCY 1 cut(s) 509
BstHHI GCGC 2 cut(s) 78, 508
BstKTI GATC 3 cut(s) 290, 523, 672
BstMAI GTCTC 1 cut(s) 5
BstMBI GATC 3 cut(s) 287, 520, 669
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstNI CCWGG 3 cut(s) 36, 503, 592
BstSCI CCNGG 3 cut(s) 34, 501, 590
BstSFI CTRYAG 2 cut(s) 61, 654
BstV1I GCAGC 2 cut(s) 252, 255
BsuRI GGCC 3 cut(s) 50, 590, 595
BtsCI GGATG 4 cut(s) 13, 214, 271, 649
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 4 cut(s) 54, 102, 156, 361
Cac8I GCNNGC 1 cut(s) 658
CaiI CAGNNNCTG 1 cut(s) 695
CfoI GCGC 2 cut(s) 78, 508
Cfr13I GGNCC 1 cut(s) 692
Csp6I GTAC 2 cut(s) 32, 535
CviAII CATG 2 cut(s) 284, 546
CviQI GTAC 2 cut(s) 32, 535
DdeI CTNAG 4 cut(s) 173, 468, 597, 666
DinI GGCGCC 1 cut(s) 507
DpnI GATC 3 cut(s) 289, 522, 671
DpnII GATC 3 cut(s) 287, 520, 669
DriI GACNNNNNGTC 1 cut(s) 202
Eam1104I CTCTTC 1 cut(s) 294
Eam1105I GACNNNNNGTC 1 cut(s) 202
EarI CTCTTC 1 cut(s) 294
Ecl136II GAGCTC 1 cut(s) 172
Eco24I GRGCYC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 692
Eco53kI GAGCTC 1 cut(s) 172
EcoICRI GAGCTC 1 cut(s) 172
EcoO109I RGGNCCY 1 cut(s) 692
EcoRI GAATTC 1 cut(s) 224
EcoRII CCWGG 3 cut(s) 34, 501, 590
EcoT38I GRGCYC 1 cut(s) 174
EgeI GGCGCC 1 cut(s) 507
EheI GGCGCC 1 cut(s) 507
FaeI CATG 2 cut(s) 287, 549
FatI CATG 2 cut(s) 283, 545
FauNDI CATATG 1 cut(s) 396
Fnu4HI GCNGC 2 cut(s) 241, 244
FokI GGATG 4 cut(s) 20, 221, 278, 636
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 2 cut(s) 241, 244
FspBI CTAG 1 cut(s) 477
GlaI GCGC 2 cut(s) 77, 507
GluI GCNGC 2 cut(s) 241, 244
GsaI CCCAGC 1 cut(s) 149
HaeII RGCGCY 1 cut(s) 509
HaeIII GGCC 3 cut(s) 50, 590, 595
HapII CCGG 1 cut(s) 73
HhaI GCGC 2 cut(s) 78, 508
Hin1I GRCGYC 1 cut(s) 506
Hin1II CATG 2 cut(s) 287, 549
Hin6I GCGC 2 cut(s) 76, 506
HinP1I GCGC 2 cut(s) 76, 506
HinfI GANTC 3 cut(s) 230, 304, 379
HpaII CCGG 1 cut(s) 73
Hpy166II GTNNAC 2 cut(s) 402, 497
Hpy188I TCNGA 6 cut(s) 140, 176, 208, 330, 378, 713
Hpy188III TCNNGA 4 cut(s) 234, 254, 383, 518
Hpy8I GTNNAC 2 cut(s) 402, 497
HpyAV CCTTC 2 cut(s) 27, 462
HpyCH4III ACNGT 3 cut(s) 348, 365, 494
HpyCH4V TGCA 5 cut(s) 194, 532, 561, 625, 656
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
HpyF3I CTNAG 4 cut(s) 173, 468, 597, 666
Hsp92I GRCGYC 1 cut(s) 506
Hsp92II CATG 2 cut(s) 287, 549
HspAI GCGC 2 cut(s) 76, 506
KasI GGCGCC 1 cut(s) 505
Kzo9I GATC 3 cut(s) 287, 520, 669
Lsp1109I GCAGC 2 cut(s) 252, 255
LweI GCATC 4 cut(s) 87, 109, 169, 658
MaeI CTAG 1 cut(s) 477
MalI GATC 3 cut(s) 289, 522, 671
MboI GATC 3 cut(s) 287, 520, 669
MboII GAAGA 3 cut(s) 12, 311, 586
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 3 cut(s) 224, 480, 707
Mly113I GGCGCC 1 cut(s) 506
MlyI GAGTC 3 cut(s) 224, 313, 388
MmeI TCCRAC 3 cut(s) 67, 186, 593
MnlI CCTC 9 cut(s) 118, 147, 364, 384, 535, 606, 636, 639, 673
MseI TTAA 1 cut(s) 114
MslI CAYNNNNRTG 1 cut(s) 366
MspI CCGG 1 cut(s) 73
MspR9I CCNGG 3 cut(s) 36, 503, 592
MvaI CCWGG 3 cut(s) 36, 503, 592
MwoI GCNNNNNNNGC 1 cut(s) 169
NarI GGCGCC 1 cut(s) 506
NdeI CATATG 1 cut(s) 396
NdeII GATC 3 cut(s) 287, 520, 669
NlaIII CATG 2 cut(s) 287, 549
NlaIV GGNNCC 2 cut(s) 507, 661
PflMI CCANNNNNTGG 1 cut(s) 322
PkrI GCNGC 2 cut(s) 242, 245
PleI GAGTC 3 cut(s) 224, 312, 387
PluTI GGCGCC 1 cut(s) 509
PpsI GAGTC 3 cut(s) 224, 312, 387
PpuMI RGGWCCY 1 cut(s) 692
Psp124BI GAGCTC 1 cut(s) 174
Psp5II RGGWCCY 1 cut(s) 692
Psp6I CCWGG 3 cut(s) 34, 501, 590
PspFI CCCAGC 1 cut(s) 145
PspGI CCWGG 3 cut(s) 34, 501, 590
PspN4I GGNNCC 2 cut(s) 507, 661
PspPI GGNCC 1 cut(s) 692
PspPPI RGGWCCY 1 cut(s) 692
PstI CTGCAG 1 cut(s) 658
PstNI CAGNNNCTG 1 cut(s) 695
RsaI GTAC 2 cut(s) 33, 536
RsaNI GTAC 2 cut(s) 32, 535
RseI CAYNNNNRTG 1 cut(s) 366
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 1 cut(s) 114
SatI GCNGC 2 cut(s) 241, 244
Sau3AI GATC 3 cut(s) 287, 520, 669
Sau96I GGNCC 1 cut(s) 692
SbfI CCTGCAGG 1 cut(s) 658
SchI GAGTC 3 cut(s) 224, 313, 388
ScrFI CCNGG 3 cut(s) 36, 503, 592
SdaI CCTGCAGG 1 cut(s) 658
SduI GDGCHC 1 cut(s) 174
SfaNI GCATC 4 cut(s) 87, 109, 169, 658
SfcI CTRYAG 2 cut(s) 61, 654
SfoI GGCGCC 1 cut(s) 507
SinI GGWCC 1 cut(s) 692
SmiMI CAYNNNNRTG 1 cut(s) 366
Sse8387I CCTGCAGG 1 cut(s) 658
Sse9I AATT 3 cut(s) 224, 480, 707
SsiI CCGC 1 cut(s) 122
SspDI GGCGCC 1 cut(s) 505
SspMI CTAG 1 cut(s) 477
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 3 cut(s) 34, 501, 590
TaaI ACNGT 3 cut(s) 348, 365, 494
TaqI TCGA 1 cut(s) 228
TasI AATT 3 cut(s) 224, 480, 707
TatI WGTACW 1 cut(s) 534
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TscAI CASTG 4 cut(s) 61, 102, 156, 368
TseI GCWGC 2 cut(s) 240, 243
TspDTI ATGAA 4 cut(s) 17, 230, 329, 419
TspRI CASTG 4 cut(s) 61, 102, 156, 368
Van91I CCANNNNNTGG 1 cut(s) 322
VpaK11BI GGWCC 1 cut(s) 692
XapI RAATTY 2 cut(s) 224, 707
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.