Rh4DG182500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001941
Physical Location & Seq
Reverse (-)
36228964 .. 36229239
276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG182500.1

Sequence Viewer

Length: 276 bp
ATGGAGAAAATTTCAGGCTTCAGTCTCTTCATCTTCGTCTTCGTTGTCTTCGGAGACATTGTGTCTTTTGAGGGGAGTTTGGGCGCTGCTAGGGTTGTGTCGGTGGGCCAACAAACTGTTGGGACGACATCAGAGGATGGCGATGATTACTGGGAGGAGTGGGATTCGGTAGAAGCCATAGTGATCTCTGCAGGCTGCATCTTTTCGGCGGTGCTGCTGATGCGGTGGTCAAATGGTGGTTGCGAAAGGGGGCTTTCCTCCGGCCGGATTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

91

Amino Acids

9.75

Weight (kDa)

4.16

Isoelectric Point (pI)

18.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 209, 223
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 1 cut(s) 9
AcuI CTGAAG 1 cut(s) 4
AfiI CCNNNNNNNGG 1 cut(s) 264
AhdI GACNNNNNGTC 1 cut(s) 61
Alw26I GTCTC 2 cut(s) 29, 48
AoxI GGCC 2 cut(s) 106, 262
ApeKI GCWGC 3 cut(s) 86, 195, 214
ApoI RAATTY 1 cut(s) 9
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 106
BbsI GAAGAC 2 cut(s) 31, 40
BbvI GCAGC 3 cut(s) 73, 182, 201
BccI CCATC 1 cut(s) 131
BcoDI GTCTC 2 cut(s) 29, 48
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 189
BfoI RGCGCY 1 cut(s) 87
BisI GCNGC 3 cut(s) 87, 196, 215
BlsI GCNGC 3 cut(s) 88, 197, 216
BmeRI GACNNNNNGTC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 106
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 2 cut(s) 207, 210
BmuI ACTGGG 1 cut(s) 160
BpiI GAAGAC 2 cut(s) 31, 40
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse1I ACTGG 1 cut(s) 155
BseGI GGATG 1 cut(s) 142
BseLI CCNNNNNNNGG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 1 cut(s) 170
BseX3I CGGCCG 1 cut(s) 262
BseXI GCAGC 3 cut(s) 73, 182, 201
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 2 cut(s) 108, 264
BsiEI CGRYCG 1 cut(s) 265
BsiSI CCGG 2 cut(s) 261, 265
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 2 cut(s) 29, 48
BsmFI GGGAC 1 cut(s) 136
BsnI GGCC 2 cut(s) 108, 264
Bsp143I GATC 1 cut(s) 183
BspACI CCGC 2 cut(s) 209, 223
BspANI GGCC 2 cut(s) 108, 264
BspMAI CTGCAG 1 cut(s) 193
BsrI ACTGG 1 cut(s) 155
BssMI GATC 1 cut(s) 183
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 193
BstF5I GGATG 1 cut(s) 142
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 1 cut(s) 186
BstMAI GTCTC 2 cut(s) 29, 48
BstMBI GATC 1 cut(s) 183
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 1 cut(s) 220
BstSFI CTRYAG 1 cut(s) 189
BstV1I GCAGC 3 cut(s) 73, 182, 201
BstV2I GAAGAC 2 cut(s) 31, 40
BstZI CGGCCG 1 cut(s) 262
BsuRI GGCC 2 cut(s) 108, 264
BtgZI GCGATG 1 cut(s) 156
BtsCI GGATG 1 cut(s) 142
Cac8I GCNNGC 1 cut(s) 193
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 106
CviAII CATG 1 cut(s) 273
CviJI RGCY 6 cut(s) 18, 108, 176, 195, 253, 264
CviKI_1 RGCY 6 cut(s) 18, 108, 176, 195, 253, 264
DpnI GATC 1 cut(s) 185
DpnII GATC 1 cut(s) 183
DriI GACNNNNNGTC 1 cut(s) 61
EaeI YGGCCR 1 cut(s) 262
EagI CGGCCG 1 cut(s) 262
Eam1104I CTCTTC 1 cut(s) 32
Eam1105I GACNNNNNGTC 1 cut(s) 61
EarI CTCTTC 1 cut(s) 32
EclXI CGGCCG 1 cut(s) 262
Eco52I CGGCCG 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 4
FaeI CATG 1 cut(s) 276
FaiI YATR 2 cut(s) 179, 274
FaqI GGGAC 1 cut(s) 136
FatI CATG 1 cut(s) 272
Fnu4HI GCNGC 3 cut(s) 87, 196, 215
FokI GGATG 1 cut(s) 149
Fsp4HI GCNGC 3 cut(s) 87, 196, 215
FspBI CTAG 1 cut(s) 90
GlaI GCGC 1 cut(s) 85
GluI GCNGC 3 cut(s) 87, 196, 215
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 2 cut(s) 108, 264
HapII CCGG 2 cut(s) 261, 265
HhaI GCGC 1 cut(s) 86
Hin1II CATG 1 cut(s) 276
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HinfI GANTC 1 cut(s) 164
HpaII CCGG 2 cut(s) 261, 265
Hpy188I TCNGA 2 cut(s) 53, 133
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4V TGCA 3 cut(s) 191, 198, 272
HpyF10VI GCNNNNNNNGC 1 cut(s) 220
Hsp92II CATG 1 cut(s) 276
HspAI GCGC 1 cut(s) 84
Kzo9I GATC 1 cut(s) 183
LpnPI CCDG 2 cut(s) 136, 177
Lsp1109I GCAGC 3 cut(s) 73, 182, 201
LweI GCATC 2 cut(s) 207, 210
MaeI CTAG 1 cut(s) 90
MalI GATC 1 cut(s) 185
MboI GATC 1 cut(s) 183
MboII GAAGA 4 cut(s) 19, 25, 31, 40
MluCI AATT 1 cut(s) 9
MnlI CCTC 4 cut(s) 64, 127, 148, 268
MspI CCGG 2 cut(s) 261, 265
MwoI GCNNNNNNNGC 1 cut(s) 220
NdeII GATC 1 cut(s) 183
NlaIII CATG 1 cut(s) 276
PfeI GAWTC 1 cut(s) 164
PkrI GCNGC 3 cut(s) 88, 197, 216
PspPI GGNCC 1 cut(s) 106
PstI CTGCAG 1 cut(s) 193
SatI GCNGC 3 cut(s) 87, 196, 215
Sau3AI GATC 1 cut(s) 183
Sau96I GGNCC 1 cut(s) 106
SfaNI GCATC 2 cut(s) 207, 210
SfcI CTRYAG 1 cut(s) 189
SgeI CNNG 4 cut(s) 27, 102, 163, 204
Sse9I AATT 1 cut(s) 9
SsiI CCGC 2 cut(s) 209, 223
SspMI CTAG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 118
TasI AATT 1 cut(s) 9
TfiI GAWTC 1 cut(s) 164
TseI GCWGC 3 cut(s) 86, 195, 214
TspDTI ATGAA 1 cut(s) 19
XapI RAATTY 1 cut(s) 9
XcmI CCANNNNNNNNNTGG 1 cut(s) 116
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.