Rh5DG558400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001941
Physical Location & Seq
Reverse (-)
86081196 .. 86081654
459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG558400.1

Sequence Viewer

Length: 459 bp
ATGGAGAAAATTTCAGGTTTTTGCCTTTTCATCTTCGTCTTCGTTGTTTTCGGAGGCAGTGCATCTGTTGAGCGGAGTTTGGGCGCTGTTGGGGCTGTGTCGGTGGGCCAATTTACTGTTGCTACACCATCAGAGGTAGGCGGTGATTTCTGGGAGGAGTGGGGTCCAGGAAGCAGACCCGGTGATCTCAGGGGTTTGGGGCGTGGTCTCACTGCAGATGCGGTGGTGATTGGCACTTGGCCGGATCGATGGTCGAAGGATAAGGGCGGTGGGAACCTGGTTTCTTCTTCTGCCGGTGGGTTTGGATCTTCATCTACTTTGGAGGGGATTGGTGGTATCCCTCTACAATTCTGTGACTACGACGATATTGGGACGGGGCTGAAGAACGTCAGATCCTGGTGCGAGGTGAGGGGATTGGGTTCTGGTGGCGATGGCCGAGAGGTGGTGTCGGCTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

15.6

Weight (kDa)

4.65

Isoelectric Point (pI)

31.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 73
AciI CCGC 4 cut(s) 73, 141, 221, 267
AclWI GGATC 3 cut(s) 252, 313, 387
AcoI YGGCCR 2 cut(s) 239, 433
AcsI RAATTY 1 cut(s) 9
AcuI CTGAAG 1 cut(s) 401
AfiI CCNNNNNNNGG 1 cut(s) 442
AjnI CCWGG 3 cut(s) 166, 276, 395
AloI GAACNNNNNNTCC 2 cut(s) 377, 409
Alw26I GTCTC 1 cut(s) 212
AlwI GGATC 3 cut(s) 252, 313, 387
AlwNI CAGNNNCTG 1 cut(s) 396
AoxI GGCC 3 cut(s) 106, 239, 433
ApoI RAATTY 1 cut(s) 9
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 106, 164
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 4 cut(s) 155, 194, 238, 418
AvaII GGWCC 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 31
BccI CCATC 3 cut(s) 136, 243, 425
BciT130I CCWGG 3 cut(s) 168, 278, 397
BciVI GTATCC 1 cut(s) 347
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 212
BfmI CTRYAG 1 cut(s) 213
BfoI RGCGCY 1 cut(s) 87
BfuI GTATCC 1 cut(s) 347
Bme1390I CCNGG 4 cut(s) 168, 180, 278, 397
Bme18I GGWCC 1 cut(s) 164
BmgT120I GGNCC 2 cut(s) 106, 164
BmiI GGNNCC 2 cut(s) 165, 275
BmrFI CCNGG 4 cut(s) 168, 180, 278, 397
BmsI GCATC 2 cut(s) 71, 208
BpiI GAAGAC 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 180
Bsa29I ATCGAT 1 cut(s) 247
BsaBI GATNNNNATC 1 cut(s) 310
BsaI GGTCTC 1 cut(s) 212
Bsc4I CCNNNNNNNGG 1 cut(s) 442
Bse118I RCCGGY 1 cut(s) 293
Bse8I GATNNNNATC 1 cut(s) 310
BseBI CCWGG 3 cut(s) 168, 278, 397
BseCI ATCGAT 1 cut(s) 247
BseJI GATNNNNATC 1 cut(s) 310
BseLI CCNNNNNNNGG 1 cut(s) 442
BseMII CTCAG 1 cut(s) 202
BseRI GAGGAG 1 cut(s) 170
BshFI GGCC 3 cut(s) 108, 241, 435
BshVI ATCGAT 1 cut(s) 247
BsiSI CCGG 3 cut(s) 180, 242, 294
BslFI GGGAC 1 cut(s) 385
BslI CCNNNNNNNGG 1 cut(s) 442
BsmAI GTCTC 1 cut(s) 212
BsmFI GGGAC 1 cut(s) 385
BsnI GGCC 3 cut(s) 108, 241, 435
Bso31I GGTCTC 1 cut(s) 212
Bsp143I GATC 4 cut(s) 184, 244, 305, 392
BspACI CCGC 4 cut(s) 73, 141, 221, 267
BspANI GGCC 3 cut(s) 108, 241, 435
BspCNI CTCAG 1 cut(s) 201
BspDI ATCGAT 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 165, 275
BspMAI CTGCAG 1 cut(s) 217
BspPI GGATC 3 cut(s) 252, 313, 387
BspTNI GGTCTC 1 cut(s) 212
BsrBI CCGCTC 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 293
BssAI RCCGGY 1 cut(s) 293
BssMI GATC 4 cut(s) 184, 244, 305, 392
Bst2UI CCWGG 3 cut(s) 168, 278, 397
Bst4CI ACNGT 1 cut(s) 118
BstDEI CTNAG 1 cut(s) 188
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 4 cut(s) 187, 247, 308, 395
BstMAI GTCTC 1 cut(s) 212
BstMBI GATC 4 cut(s) 184, 244, 305, 392
BstMWI GCNNNNNNNGC 1 cut(s) 92
BstNI CCWGG 3 cut(s) 168, 278, 397
BstSCI CCNGG 4 cut(s) 166, 178, 276, 395
BstSFI CTRYAG 1 cut(s) 213
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 2 cut(s) 305, 392
BstYI RGATCY 2 cut(s) 305, 392
Bsu15I ATCGAT 1 cut(s) 247
BsuI GTATCC 1 cut(s) 347
BsuRI GGCC 3 cut(s) 108, 241, 435
BsuTUI ATCGAT 1 cut(s) 247
BtgZI GCGATG 1 cut(s) 444
BtsI GCAGTG 2 cut(s) 64, 210
BtsIMutI CAGTG 2 cut(s) 64, 210
CaiI CAGNNNCTG 1 cut(s) 396
CfoI GCGC 1 cut(s) 86
Cfr10I RCCGGY 1 cut(s) 293
Cfr13I GGNCC 2 cut(s) 106, 164
ClaI ATCGAT 1 cut(s) 247
CsiI ACCWGGT 1 cut(s) 276
CviJI RGCY 6 cut(s) 95, 108, 241, 379, 435, 452
CviKI_1 RGCY 6 cut(s) 95, 108, 241, 379, 435, 452
DdeI CTNAG 1 cut(s) 188
DpnI GATC 4 cut(s) 186, 246, 307, 394
DpnII GATC 4 cut(s) 184, 244, 305, 392
EaeI YGGCCR 2 cut(s) 239, 433
Eco31I GGTCTC 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 164
Eco57I CTGAAG 1 cut(s) 401
EcoRII CCWGG 3 cut(s) 166, 276, 395
FaqI GGGAC 1 cut(s) 385
GlaI GCGC 1 cut(s) 85
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 3 cut(s) 108, 241, 435
HapII CCGG 3 cut(s) 180, 242, 294
HhaI GCGC 1 cut(s) 86
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HpaII CCGG 3 cut(s) 180, 242, 294
HphI GGTGA 4 cut(s) 155, 194, 238, 418
Hpy188I TCNGA 3 cut(s) 53, 133, 392
Hpy99I CGWCG 1 cut(s) 365
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 2 cut(s) 62, 215
HpyF10VI GCNNNNNNNGC 1 cut(s) 92
HpyF3I CTNAG 1 cut(s) 188
HpySE526I ACGT 1 cut(s) 387
HspAI GCGC 1 cut(s) 84
Kzo9I GATC 4 cut(s) 184, 244, 305, 392
LweI GCATC 2 cut(s) 71, 208
MabI ACCWGGT 1 cut(s) 276
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 1 cut(s) 353
MalI GATC 4 cut(s) 186, 246, 307, 394
MbiI CCGCTC 1 cut(s) 73
MboI GATC 4 cut(s) 184, 244, 305, 392
MboII GAAGA 6 cut(s) 25, 31, 276, 279, 300, 394
MflI RGATCY 2 cut(s) 305, 392
MluCI AATT 3 cut(s) 9, 110, 347
MnlI CCTC 8 cut(s) 47, 127, 148, 316, 351, 397, 402, 433
MspI CCGG 3 cut(s) 180, 242, 294
MspR9I CCNGG 4 cut(s) 168, 180, 278, 397
MvaI CCWGG 3 cut(s) 168, 278, 397
MwoI GCNNNNNNNGC 1 cut(s) 92
NciI CCSGG 1 cut(s) 180
NdeII GATC 4 cut(s) 184, 244, 305, 392
NlaIV GGNNCC 2 cut(s) 165, 275
NmuCI GTSAC 1 cut(s) 353
PfoI TCCNGGA 1 cut(s) 166
Psp6I CCWGG 3 cut(s) 166, 276, 395
PspGI CCWGG 3 cut(s) 166, 276, 395
PspN4I GGNNCC 2 cut(s) 165, 275
PspPI GGNCC 2 cut(s) 106, 164
PstI CTGCAG 1 cut(s) 217
PstNI CAGNNNCTG 1 cut(s) 396
PsuI RGATCY 2 cut(s) 305, 392
Sau3AI GATC 4 cut(s) 184, 244, 305, 392
Sau96I GGNCC 2 cut(s) 106, 164
ScrFI CCNGG 4 cut(s) 168, 180, 278, 397
SetI ASST 6 cut(s) 19, 138, 279, 390, 408, 444
SexAI ACCWGGT 1 cut(s) 276
SfaNI GCATC 2 cut(s) 71, 208
SfcI CTRYAG 1 cut(s) 213
SinI GGWCC 1 cut(s) 164
Sse9I AATT 3 cut(s) 9, 110, 347
SsiI CCGC 4 cut(s) 73, 141, 221, 267
StyD4I CCNGG 4 cut(s) 166, 178, 276, 395
TaaI ACNGT 1 cut(s) 118
TaiI ACGT 1 cut(s) 390
TaqI TCGA 2 cut(s) 247, 254
TasI AATT 3 cut(s) 9, 110, 347
TscAI CASTG 2 cut(s) 64, 217
TseFI GTSAC 1 cut(s) 353
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 2 cut(s) 19, 300
TspRI CASTG 2 cut(s) 64, 217
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.