Rh5AG258800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001941
Physical Location & Seq
Forward (+)
33776425 .. 33778108
1684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG258800.1

Sequence Viewer

Length: 978 bp
ATGGAGAAATTTTCAGGCTTCGGCCTCTTCATTTTTACTTTCGTTGTCTTCAGAGGTAGTGCCTCTTTGGAGAGAAGTTTGGGCGTTGCTGGGGCGGTGTCGGGGGGCCAATTTTCTTTTGGTATGGGTTCGGAGGTAGGCGGCTATTACTGGGAGGAGTGGGATCCGGGTAGATGCTACAGCTTTCTCAATGGGTTGCCTCTTCTCGGCAGGGCTGCTGATGCGGGGGCGAATCAATGGGTGCGGAAGGGGGTCTTCCTCTGGCTGGATTGCGTGTCGGTGTGGGTGTTGTGTCCCACTAGTAGAGCTTCACTTTGTGGTGTTGGTGACTGTTTTTGGGTCCCCGACGTTCTTCGATGCCAAGTTTCTGGTGGGGTTTTCAACGGTGAAGGACGCATAGTGCAGGATGACATTGATTCAGTCTGGCGGTGGTCGTACCACAGGTCAATTGGTGGTGCAGATCTGGCTCTAGATTGGGTTGAGGCTCTTCCCTATGCGGCGTTCGTCGTCAGTCAGATTTGGAGTCCAAACTGGCCTTCTCAAAAGATGTTGGCATGCGCAGTTCGGCGTCGTCAGAAGCAAATTGTCGGGTTTACTTGGAGTACTCCAGGTAACACATGGTGCTGGGCTTGGAGGAAGGGTCTACATCAGATCCGAATATGGGATCCAAGTGGGTCTTGGACCCGACCCAGGATCTTGTCACAACTTTTCTATTGGGTTCCCTTCCAATTATTGCACTGGCTTTGGGATCCTGATGTTTATTTGTATTGGGTTGAGGACTCAAGATTATGGTATTTGTTCGAATGGGATCGTATGCCAACACAGCTATGTTCTGACACCCTTGGCCGGCTTCAATCTTTTGATAGAAGATTGCCCTCTGTTCTTTCTCATATTGACTTTTGTCATTATGTGAGTTGGATATGCATGGGTAGTCATACCATCGACTACAACATTCACTACATGGCAGTAATTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

325

Amino Acids

37.48

Weight (kDa)

6.64

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 559
AccI GTMKAC 1 cut(s) 643
AciI CCGC 6 cut(s) 95, 141, 224, 244, 427, 497
AclWI GGATC 9 cut(s) 158, 171, 646, 659, 672, 701, 743, 756, 816
AcoI YGGCCR 1 cut(s) 844
AcsI RAATTY 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 34
AcyI GRCGYC 1 cut(s) 568
AdeI CACNNNGTG 2 cut(s) 317, 621
AfaI GTAC 2 cut(s) 437, 604
AfiI CCNNNNNNNGG 5 cut(s) 206, 265, 661, 690, 846
AgsI TTSAA 2 cut(s) 382, 854
AhlI ACTAGT 1 cut(s) 299
AjnI CCWGG 2 cut(s) 607, 689
AjuI GAANNNNNNNTTGG 2 cut(s) 520, 552
AluBI AGCT 3 cut(s) 183, 308, 826
AluI AGCT 3 cut(s) 183, 308, 826
AlwI GGATC 9 cut(s) 158, 171, 646, 659, 672, 701, 743, 756, 816
AoxI GGCC 4 cut(s) 22, 106, 533, 844
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 1 cut(s) 8
AspLEI GCGC 1 cut(s) 560
AspS9I GGNCC 3 cut(s) 106, 340, 681
AsuC2I CCSGG 1 cut(s) 168
AsuHPI GGTGA 2 cut(s) 338, 398
AsuII TTCGAA 1 cut(s) 801
AvaII GGWCC 2 cut(s) 340, 681
BamHI GGATCC 3 cut(s) 163, 664, 748
BbsI GAAGAC 2 cut(s) 40, 247
BbvI GCAGC 1 cut(s) 202
BccI CCATC 1 cut(s) 947
BciT130I CCWGG 2 cut(s) 609, 691
BcnI CCSGG 1 cut(s) 168
BcuI ACTAGT 1 cut(s) 299
BfaI CTAG 2 cut(s) 300, 470
BfmI CTRYAG 1 cut(s) 178
BglII AGATCT 1 cut(s) 460
BisI GCNGC 3 cut(s) 142, 216, 498
BlsI GCNGC 3 cut(s) 143, 217, 499
BmcAI AGTACT 1 cut(s) 604
Bme1390I CCNGG 3 cut(s) 168, 609, 691
Bme18I GGWCC 2 cut(s) 340, 681
BmgT120I GGNCC 3 cut(s) 106, 340, 681
BmiI GGNNCC 8 cut(s) 107, 165, 341, 342, 666, 683, 720, 750
BmrFI CCNGG 3 cut(s) 168, 609, 691
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 3 cut(s) 164, 211, 347
BmuI ACTGGG 1 cut(s) 160
BoxI GACNNNNGTC 1 cut(s) 900
BpiI GAAGAC 2 cut(s) 40, 247
BpmI CTGGAG 1 cut(s) 591
Bpu14I TTCGAA 1 cut(s) 801
BpuEI CTTGAG 1 cut(s) 766
BpuMI CCSGG 1 cut(s) 168
BsaHI GRCGYC 1 cut(s) 568
BsaJI CCNNGG 2 cut(s) 689, 841
BsaXI ACNNNNNCTCC 2 cut(s) 625, 655
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 265, 661, 690, 846
Bse118I RCCGGY 1 cut(s) 846
Bse1I ACTGG 3 cut(s) 155, 536, 743
BseBI CCWGG 2 cut(s) 609, 691
BseDI CCNNGG 2 cut(s) 689, 841
BseGI GGATG 1 cut(s) 412
BseLI CCNNNNNNNGG 5 cut(s) 206, 265, 661, 690, 846
BseNI ACTGG 3 cut(s) 155, 536, 743
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 1 cut(s) 202
BseYI CCCAGC 2 cut(s) 89, 624
BsgI GTGCAG 2 cut(s) 422, 477
BshFI GGCC 4 cut(s) 24, 108, 535, 846
BsiSI CCGG 2 cut(s) 167, 847
BslFI GGGAC 2 cut(s) 279, 326
BslI CCNNNNNNNGG 5 cut(s) 206, 265, 661, 690, 846
BsmFI GGGAC 2 cut(s) 279, 326
BsnI GGCC 4 cut(s) 24, 108, 535, 846
Bsp119I TTCGAA 1 cut(s) 801
Bsp143I GATC 7 cut(s) 163, 460, 651, 664, 693, 748, 808
BspACI CCGC 6 cut(s) 95, 141, 224, 244, 427, 497
BspANI GGCC 4 cut(s) 24, 108, 535, 846
BspLI GGNNCC 8 cut(s) 107, 165, 341, 342, 666, 683, 720, 750
BspPI GGATC 9 cut(s) 158, 171, 646, 659, 672, 701, 743, 756, 816
BspQI GCTCTTC 1 cut(s) 492
BspT104I TTCGAA 1 cut(s) 801
BsrFI RCCGGY 1 cut(s) 846
BsrI ACTGG 3 cut(s) 155, 536, 743
BssAI RCCGGY 1 cut(s) 846
BssECI CCNNGG 2 cut(s) 689, 841
BssMI GATC 7 cut(s) 163, 460, 651, 664, 693, 748, 808
BssNI GRCGYC 1 cut(s) 568
BssT1I CCWWGG 1 cut(s) 841
Bst2UI CCWGG 2 cut(s) 609, 691
Bst4CI ACNGT 2 cut(s) 332, 386
Bst6I CTCTTC 3 cut(s) 32, 207, 492
BstACI GRCGYC 1 cut(s) 568
BstBI TTCGAA 1 cut(s) 801
BstC8I GCNNGC 2 cut(s) 556, 848
BstF5I GGATG 1 cut(s) 412
BstHHI GCGC 1 cut(s) 560
BstKTI GATC 7 cut(s) 166, 463, 654, 667, 696, 751, 811
BstMBI GATC 7 cut(s) 163, 460, 651, 664, 693, 748, 808
BstMWI GCNNNNNNNGC 3 cut(s) 221, 464, 823
BstNI CCWGG 2 cut(s) 609, 691
BstNSI RCATGY 1 cut(s) 558
BstPAI GACNNNNGTC 1 cut(s) 900
BstSCI CCNGG 3 cut(s) 166, 607, 689
BstSFI CTRYAG 1 cut(s) 178
BstV1I GCAGC 1 cut(s) 202
BstV2I GAAGAC 2 cut(s) 40, 247
BstX2I RGATCY 6 cut(s) 163, 460, 651, 664, 693, 748
BstXI CCANNNNNNTGG 1 cut(s) 368
BstYI RGATCY 6 cut(s) 163, 460, 651, 664, 693, 748
BsuRI GGCC 4 cut(s) 24, 108, 535, 846
BtsCI GGATG 1 cut(s) 412
BtsIMutI CAGTG 1 cut(s) 736
Cac8I GCNNGC 2 cut(s) 556, 848
CfoI GCGC 1 cut(s) 560
Cfr10I RCCGGY 1 cut(s) 846
Cfr13I GGNCC 3 cut(s) 106, 340, 681
CseI GACGC 2 cut(s) 402, 557
Csp6I GTAC 2 cut(s) 436, 603
CviAII CATG 4 cut(s) 555, 618, 925, 961
CviQI GTAC 2 cut(s) 436, 603
DpnI GATC 7 cut(s) 165, 462, 653, 666, 695, 750, 810
DpnII GATC 7 cut(s) 163, 460, 651, 664, 693, 748, 808
DraIII CACNNNGTG 2 cut(s) 317, 621
EaeI YGGCCR 1 cut(s) 844
Eam1104I CTCTTC 3 cut(s) 32, 207, 492
EarI CTCTTC 3 cut(s) 32, 207, 492
Eco130I CCWWGG 1 cut(s) 841
Eco47I GGWCC 2 cut(s) 340, 681
Eco57I CTGAAG 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 340
EcoRII CCWGG 2 cut(s) 607, 689
EcoT14I CCWWGG 1 cut(s) 841
EcoT22I ATGCAT 1 cut(s) 926
ErhI CCWWGG 1 cut(s) 841
FaeI CATG 4 cut(s) 558, 621, 928, 964
FalI AAGNNNNNCTT 4 cut(s) 239, 271, 661, 693
FaqI GGGAC 2 cut(s) 279, 326
FatI CATG 4 cut(s) 554, 617, 924, 960
FauI CCCGC 1 cut(s) 217
FblI GTMKAC 1 cut(s) 643
Fnu4HI GCNGC 3 cut(s) 142, 216, 498
FokI GGATG 1 cut(s) 419
Fsp4HI GCNGC 3 cut(s) 142, 216, 498
FspBI CTAG 2 cut(s) 300, 470
FspI TGCGCA 1 cut(s) 559
GlaI GCGC 1 cut(s) 559
GluI GCNGC 3 cut(s) 142, 216, 498
GsaI CCCAGC 2 cut(s) 93, 628
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 4 cut(s) 24, 108, 535, 846
HapII CCGG 2 cut(s) 167, 847
HgaI GACGC 2 cut(s) 402, 557
HhaI GCGC 1 cut(s) 560
Hin1I GRCGYC 1 cut(s) 568
Hin1II CATG 4 cut(s) 558, 621, 928, 964
Hin6I GCGC 1 cut(s) 558
HinP1I GCGC 1 cut(s) 558
HinfI GANTC 4 cut(s) 232, 416, 523, 779
HpaII CCGG 2 cut(s) 167, 847
HphI GGTGA 2 cut(s) 338, 398
Hpy166II GTNNAC 2 cut(s) 594, 644
Hpy188I TCNGA 7 cut(s) 53, 133, 516, 576, 651, 656, 835
Hpy188III TCNNGA 3 cut(s) 470, 752, 783
Hpy8I GTNNAC 2 cut(s) 594, 644
Hpy99I CGWCG 3 cut(s) 350, 509, 573
HpyAV CCTTC 5 cut(s) 241, 383, 546, 631, 733
HpyCH4III ACNGT 2 cut(s) 332, 386
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 4 cut(s) 403, 458, 736, 924
HpyF10VI GCNNNNNNNGC 3 cut(s) 221, 464, 823
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 1 cut(s) 568
Hsp92II CATG 4 cut(s) 558, 621, 928, 964
HspAI GCGC 1 cut(s) 558
KflI GGGWCCC 1 cut(s) 340
KroI GCCGGC 1 cut(s) 846
KroNI GCCGGC 1 cut(s) 848
Kzo9I GATC 7 cut(s) 163, 460, 651, 664, 693, 748, 808
LguI GCTCTTC 1 cut(s) 492
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 3 cut(s) 164, 211, 347
MaeI CTAG 2 cut(s) 300, 470
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 3 cut(s) 326, 611, 699
MalI GATC 7 cut(s) 165, 462, 653, 666, 695, 750, 810
MboI GATC 7 cut(s) 163, 460, 651, 664, 693, 748, 808
MboII GAAGA 7 cut(s) 19, 40, 194, 247, 344, 479, 879
MfeI CAATTG 1 cut(s) 447
MflI RGATCY 6 cut(s) 163, 460, 651, 664, 693, 748
MluCI AATT 6 cut(s) 8, 110, 447, 582, 728, 969
MlyI GAGTC 2 cut(s) 532, 773
MmeI TCCRAC 1 cut(s) 896
Mph1103I ATGCAT 1 cut(s) 926
MroNI GCCGGC 1 cut(s) 846
MslI CAYNNNNRTG 1 cut(s) 826
MspI CCGG 2 cut(s) 167, 847
MspR9I CCNGG 3 cut(s) 168, 609, 691
MunI CAATTG 1 cut(s) 447
MvaI CCWGG 2 cut(s) 609, 691
MwoI GCNNNNNNNGC 3 cut(s) 221, 464, 823
NaeI GCCGGC 1 cut(s) 848
NciI CCSGG 1 cut(s) 168
NdeII GATC 7 cut(s) 163, 460, 651, 664, 693, 748, 808
NgoMIV GCCGGC 1 cut(s) 846
NlaIII CATG 4 cut(s) 558, 621, 928, 964
NlaIV GGNNCC 8 cut(s) 107, 165, 341, 342, 666, 683, 720, 750
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 2 cut(s) 326, 699
NsbI TGCGCA 1 cut(s) 559
NsiI ATGCAT 1 cut(s) 926
NspI RCATGY 1 cut(s) 558
NspV TTCGAA 1 cut(s) 801
PaeI GCATGC 1 cut(s) 558
PciSI GCTCTTC 1 cut(s) 492
PdiI GCCGGC 1 cut(s) 848
PfeI GAWTC 2 cut(s) 232, 416
PkrI GCNGC 3 cut(s) 143, 217, 499
PleI GAGTC 2 cut(s) 531, 773
PpsI GAGTC 2 cut(s) 531, 773
PpuMI RGGWCCY 1 cut(s) 340
PshAI GACNNNNGTC 1 cut(s) 900
Psp5II RGGWCCY 1 cut(s) 340
Psp6I CCWGG 2 cut(s) 607, 689
PspFI CCCAGC 2 cut(s) 89, 624
PspGI CCWGG 2 cut(s) 607, 689
PspN4I GGNNCC 8 cut(s) 107, 165, 341, 342, 666, 683, 720, 750
PspPI GGNCC 3 cut(s) 106, 340, 681
PspPPI RGGWCCY 1 cut(s) 340
PsuI RGATCY 6 cut(s) 163, 460, 651, 664, 693, 748
RsaI GTAC 2 cut(s) 437, 604
RsaNI GTAC 2 cut(s) 436, 603
RseI CAYNNNNRTG 1 cut(s) 826
SapI GCTCTTC 1 cut(s) 492
SatI GCNGC 3 cut(s) 142, 216, 498
Sau3AI GATC 7 cut(s) 163, 460, 651, 664, 693, 748, 808
Sau96I GGNCC 3 cut(s) 106, 340, 681
ScaI AGTACT 1 cut(s) 604
SchI GAGTC 2 cut(s) 532, 773
ScrFI CCNGG 3 cut(s) 168, 609, 691
SetI ASST 8 cut(s) 58, 138, 185, 310, 351, 446, 613, 828
SfaNI GCATC 3 cut(s) 164, 211, 347
SfcI CTRYAG 1 cut(s) 178
SfuI TTCGAA 1 cut(s) 801
SinI GGWCC 2 cut(s) 340, 681
SmiMI CAYNNNNRTG 1 cut(s) 826
SmlI CTYRAG 1 cut(s) 781
SmoI CTYRAG 1 cut(s) 781
SpeI ACTAGT 1 cut(s) 299
SphI GCATGC 1 cut(s) 558
Sse9I AATT 6 cut(s) 8, 110, 447, 582, 728, 969
SsiI CCGC 6 cut(s) 95, 141, 224, 244, 427, 497
SspMI CTAG 2 cut(s) 300, 470
StyD4I CCNGG 3 cut(s) 166, 607, 689
StyI CCWWGG 1 cut(s) 841
TaaI ACNGT 2 cut(s) 332, 386
TaiI ACGT 1 cut(s) 351
TaqI TCGA 3 cut(s) 355, 801, 942
TasI AATT 6 cut(s) 8, 110, 447, 582, 728, 969
TatI WGTACW 1 cut(s) 602
TauI GCSGC 2 cut(s) 144, 500
TfiI GAWTC 2 cut(s) 232, 416
TscAI CASTG 1 cut(s) 743
TseFI GTSAC 2 cut(s) 326, 699
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 2 cut(s) 326, 699
TspDTI ATGAA 1 cut(s) 19
TspRI CASTG 1 cut(s) 743
VpaK11BI GGWCC 2 cut(s) 340, 681
XapI RAATTY 1 cut(s) 8
XbaI TCTAGA 1 cut(s) 469
XceI RCATGY 1 cut(s) 558
XcmI CCANNNNNNNNNTGG 5 cut(s) 116, 368, 446, 615, 675
XmiI GTMKAC 1 cut(s) 643
XspI CTAG 2 cut(s) 300, 470
ZrmI AGTACT 1 cut(s) 604
Zsp2I ATGCAT 1 cut(s) 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.