Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0001296

Physical Location & Seq

Genomic Coordinates Reverse (-)

36859198 .. 36860028

Gene Structure 831 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG186100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 831 bp

ATGGATTTGAGATCTGAAATCGAGAGGCTCTGCAGATCTGAATCTGGTCTGAGATCGATGGATTTGAGATCTGAAATCGATCGCCTTTCTCGCAAGCGCAAGGAGGACAATCTCCGACGCGTCGTTGAGACCTCCGAAGGAAATCTCCGCATCTTCGCTCTCGCTTCCTCCTCCGACCACGACGTCGACGAAGGAGACAACGGCGGCTCCAGAAAACGCATGAGGCGACACCGCGGCTGCAACGCCGTCGTTTCATCCACCAAGCTCGCCGCGGAGCCCACCAGGCCCGTCAGATCCGCCGTGAAACCCTATGACGACGGTGGGGATCTGAATATTGGGGAGTTCGAGAAACACACAAAGGGCATCGGCATGAAATTGCAGATGAAGATGGGCTATAACGAACGTGGACTCGGCAAAAACGAGGAAGATATTGAAGCCGAATTGAGGTCCAAAAATGAAGGTCTTGGTTTTGATTACAAGGAGGGGACTAAGCTGCGGCGGCGGCCCACATCGTTTGTGAAAGCCAAGGTGGTGCACAAAAGTGTCGCTGCTGGTGACAATGATCACAAGAAGAGCAAGACAGAAATAACCAAAAAACCAAGCAGCCTAGACACTAGTACAGTGGAAGAACACAAGCCCAACGAGCAGCCTAATGAGGCAATTACCAGTGCCGCCGCCGCCACCGGCATGGAGAAGAAGACGACTACCAAAGGTGTCGATTTGCTGGGAAACGTGATGGCTGATATGAGAAGACCTCTTGATGGAATCTTGGCCGACTTTGAGAACTTGAGTTTGAAAGGTACCAGCTCCAGGTGCATCTTAGCTATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000375 GO:0000377 GO:0000390 GO:0000398 GO:0000781 GO:0000784 GO:0003674 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005694 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0022411 GO:0030154 GO:0030198 GO:0031012 GO:0031333 GO:0031974 GO:0031981 GO:0032091 GO:0032502 GO:0032984 GO:0032988 GO:0032991 GO:0034641 GO:0042752 GO:0043021 GO:0043062 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043393 GO:0043933 GO:0044087 GO:0044092 GO:0044237 GO:0044238 GO:0044421 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044877 GO:0046483 GO:0048519 GO:0048523 GO:0048869 GO:0050789 GO:0050794 GO:0051098 GO:0051100 GO:0051128 GO:0051129 GO:0065007 GO:0065009 GO:0070013 GO:0070990 GO:0071008 GO:0071013 GO:0071014 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0098687 GO:1901360 GO:1902494 GO:1990446 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-patch GCFC TIP_N

Protein Analysis

276

Amino Acids

30.64

Weight (kDa)

9.22

Isoelectric Point (pI)

61.22

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001296)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g13670
rosa_chinensis	RchiOBHm_Chr4g0414401 RchiOBHm_Chr4g0414501
rosa_laevigata	RLG00000008128 RLG00000008130 RLG00000008132 RLG00000008133 RLG00000008135 RLG00000008148 RLG00000008149 RLG00000013053
rosa_multiflora	Rmu_co8302837.1_g000001 Rmu_sc0001192.1_g000007 Rmu_sc0001192.1_g000008 Rmu_sc0001192.1_g000015 Rmu_sc0001432.1_g000009 Rmu_sc0006138.1_g000003 Rmu_sc0006138.1_g000004 Rmu_sc0006138.1_g000006 Rmu_sc0007034.1_g000001 Rmu_sc0011089.1_g000001 Rmu_sc0012211.1_g000001 Rmu_sc0012211.1_g000003
rosa_roxburghii	Rroxscaffold_1G00012060 Rroxscaffold_5G00358310 Rroxscaffold_5G00358450 Rroxscaffold_7G00187960
rosa_rugosa	Rorug02G0278500 Rorug04G0128000 Rorug04G0129200
rosa_samantha	Rh4AG186200 Rh4AG186400 Rh4AG187300 Rh4AG187400 Rh4AG187500 Rh4BG185200 Rh4CG198500 Rh4CG198700 Rh4CG199800 Rh4DG183000 Rh4DG184200 Rh4DG184800 Rh4DG185800 Rh4DG185900 Rh4DG186100 Rh4DG186300 Rh4DG186400 Rh6AG244700 Rh6BG247100 Rh6CG248700 Rh6CG248900
rosa_wichuraiana	Rw4G015900 Rw4G016010 Rw4G016030 Rw6G021190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	182
AatII	GACGTC	1 cut(s)	186
Acc65I	GGTACC	1 cut(s)	800
AccB1I	GGYRCC	1 cut(s)	800
AccI	GTMKAC	1 cut(s)	186
AccII	CGCG	3 cut(s)	120, 234, 272
AclWI	GGATC	2 cut(s)	288, 333
AcoI	YGGCCR	1 cut(s)	771
AcyI	GRCGYC	1 cut(s)	183
AfaI	GTAC	2 cut(s)	619, 802
AfiI	CCNNNNNNNGG	3 cut(s)	444, 761, 810
AflIII	ACRYGT	1 cut(s)	118
AgsI	TTSAA	2 cut(s)	434, 796
AhlI	ACTAGT	1 cut(s)	614
AjnI	CCWGG	2 cut(s)	281, 809
AleI	CACNNNNGTG	1 cut(s)	540
AluBI	AGCT	4 cut(s)	265, 493, 807, 824
AluI	AGCT	4 cut(s)	265, 493, 807, 824
Alw21I	GWGCWC	1 cut(s)	537
Alw26I	GTCTC	2 cut(s)	122, 189
Alw44I	GTGCAC	1 cut(s)	533
AlwI	GGATC	2 cut(s)	288, 333
AoxI	GGCC	3 cut(s)	284, 503, 771
ApaLI	GTGCAC	1 cut(s)	533
ApeKI	GCWGC	5 cut(s)	237, 493, 548, 603, 646
Asp718I	GGTACC	1 cut(s)	800
AspLEI	GCGC	1 cut(s)	99
AspS9I	GGNCC	3 cut(s)	285, 447, 504
AsuHPI	GGTGA	1 cut(s)	566
AvaII	GGWCC	1 cut(s)	447
BaeGI	GKGCMC	1 cut(s)	537
BanI	GGYRCC	1 cut(s)	800
BanII	GRGCYC	1 cut(s)	279
BbsI	GAAGAC	2 cut(s)	704, 757
Bbv12I	GWGCWC	1 cut(s)	537
BbvI	GCAGC	5 cut(s)	224, 480, 535, 615, 658
BccI	CCATC	4 cut(s)	52, 382, 730, 755
BceAI	ACGGC	3 cut(s)	217, 230, 284
BcgI	CGANNNNNNTGC	2 cut(s)	229, 263
BciT130I	CCWGG	2 cut(s)	283, 811
BclI	TGATCA	1 cut(s)	562
BcoDI	GTCTC	2 cut(s)	122, 189
BcuI	ACTAGT	1 cut(s)	614
BfaI	CTAG	2 cut(s)	608, 615
BfmI	CTRYAG	1 cut(s)	31
BglI	GCCNNNNNGGC	1 cut(s)	283
BglII	AGATCT	3 cut(s)	11, 35, 68
Bme1390I	CCNGG	2 cut(s)	283, 811
Bme18I	GGWCC	1 cut(s)	447
BmgT120I	GGNCC	3 cut(s)	285, 447, 504
BmiI	GGNNCC	3 cut(s)	208, 276, 802
BmrFI	CCNGG	2 cut(s)	283, 811
BmsI	GCATC	3 cut(s)	159, 372, 825
BpiI	GAAGAC	2 cut(s)	704, 757
BplI	GAGNNNNNCTC	2 cut(s)	739, 771
BpmI	CTGGAG	2 cut(s)	193, 793
BpuEI	CTTGAG	1 cut(s)	808
Bsa29I	ATCGAT	2 cut(s)	56, 78
BsaBI	GATNNNNATC	1 cut(s)	40
BsaHI	GRCGYC	1 cut(s)	183
BsaI	GGTCTC	1 cut(s)	122
BsaJI	CCNNGG	3 cut(s)	232, 270, 525
BsaXI	ACNNNNNCTCC	2 cut(s)	191, 221
Bsc4I	CCNNNNNNNGG	3 cut(s)	444, 761, 810
Bse118I	RCCGGY	1 cut(s)	683
Bse1I	ACTGG	1 cut(s)	666
Bse8I	GATNNNNATC	1 cut(s)	40
BseBI	CCWGG	2 cut(s)	283, 811
BseCI	ATCGAT	2 cut(s)	56, 78
BseDI	CCNNGG	3 cut(s)	232, 270, 525
BseGI	GGATG	1 cut(s)	254
BseJI	GATNNNNATC	1 cut(s)	40
BseLI	CCNNNNNNNGG	3 cut(s)	444, 761, 810
BseMII	CTCAG	1 cut(s)	41
BseNI	ACTGG	1 cut(s)	666
BseRI	GAGGAG	1 cut(s)	160
BseSI	GKGCMC	1 cut(s)	537
BseXI	GCAGC	5 cut(s)	224, 480, 535, 615, 658
BseYI	CCCAGC	1 cut(s)	724
Bsh1236I	CGCG	3 cut(s)	120, 234, 272
Bsh1285I	CGRYCG	1 cut(s)	82
BshFI	GGCC	3 cut(s)	286, 505, 773
BshNI	GGYRCC	1 cut(s)	800
BshVI	ATCGAT	2 cut(s)	56, 78
BsiEI	CGRYCG	1 cut(s)	82
BsiHKAI	GWGCWC	1 cut(s)	537
BsiSI	CCGG	1 cut(s)	684
BslFI	GGGAC	1 cut(s)	499
BslI	CCNNNNNNNGG	3 cut(s)	444, 761, 810
BsmAI	GTCTC	2 cut(s)	122, 189
BsmFI	GGGAC	1 cut(s)	499
BsnI	GGCC	3 cut(s)	286, 505, 773
Bso31I	GGTCTC	1 cut(s)	122
Bsp1286I	GDGCHC	2 cut(s)	279, 537
Bsp143I	GATC	8 cut(s)	11, 35, 53, 68, 79, 293, 325, 562
BspANI	GGCC	3 cut(s)	286, 505, 773
BspCNI	CTCAG	1 cut(s)	42
BspDI	ATCGAT	2 cut(s)	56, 78
BspFNI	CGCG	3 cut(s)	120, 234, 272
BspLI	GGNNCC	3 cut(s)	208, 276, 802
BspMAI	CTGCAG	1 cut(s)	35
BspPI	GGATC	2 cut(s)	288, 333
BspQI	GCTCTTC	1 cut(s)	566
BspT107I	GGYRCC	1 cut(s)	800
BspTNI	GGTCTC	1 cut(s)	122
BsrFI	RCCGGY	1 cut(s)	683
BsrI	ACTGG	1 cut(s)	666
BssAI	RCCGGY	1 cut(s)	683
BssECI	CCNNGG	3 cut(s)	232, 270, 525
BssMI	GATC	8 cut(s)	11, 35, 53, 68, 79, 293, 325, 562
BssNI	GRCGYC	1 cut(s)	183
BssT1I	CCWWGG	1 cut(s)	525
Bst2UI	CCWGG	2 cut(s)	283, 811
Bst4CI	ACNGT	2 cut(s)	320, 622
Bst6I	CTCTTC	1 cut(s)	566
BstACI	GRCGYC	1 cut(s)	183
BstC8I	GCNNGC	2 cut(s)	95, 267
BstDEI	CTNAG	3 cut(s)	50, 489, 820
BstDSI	CCRYGG	2 cut(s)	232, 270
BstF5I	GGATG	1 cut(s)	254
BstFNI	CGCG	3 cut(s)	120, 234, 272
BstHHI	GCGC	1 cut(s)	99
BstKTI	GATC	8 cut(s)	14, 38, 56, 71, 82, 296, 328, 565
BstMAI	GTCTC	2 cut(s)	122, 189
BstMBI	GATC	8 cut(s)	11, 35, 53, 68, 79, 293, 325, 562
BstMCI	CGRYCG	1 cut(s)	82
BstMWI	GCNNNNNNNGC	7 cut(s)	90, 283, 499, 502, 643, 677, 813
BstNI	CCWGG	2 cut(s)	283, 811
BstSCI	CCNGG	2 cut(s)	281, 809
BstSFI	CTRYAG	1 cut(s)	31
BstSLI	GKGCMC	1 cut(s)	537
BstUI	CGCG	3 cut(s)	120, 234, 272
BstV1I	GCAGC	5 cut(s)	224, 480, 535, 615, 658
BstV2I	GAAGAC	2 cut(s)	704, 757
BstX2I	RGATCY	5 cut(s)	11, 35, 68, 293, 325
BstXI	CCANNNNNNTGG	1 cut(s)	688
BstYI	RGATCY	5 cut(s)	11, 35, 68, 293, 325
Bsu15I	ATCGAT	2 cut(s)	56, 78
BsuRI	GGCC	3 cut(s)	286, 505, 773
BsuTUI	ATCGAT	2 cut(s)	56, 78
BtgI	CCRYGG	2 cut(s)	232, 270
BtsCI	GGATG	1 cut(s)	254
BtsIMutI	CAGTG	2 cut(s)	627, 673
Cac8I	GCNNGC	2 cut(s)	95, 267
CfoI	GCGC	1 cut(s)	99
Cfr10I	RCCGGY	1 cut(s)	683
Cfr13I	GGNCC	3 cut(s)	285, 447, 504
Cfr42I	CCGCGG	2 cut(s)	235, 273
ClaI	ATCGAT	2 cut(s)	56, 78
CseI	GACGC	2 cut(s)	109, 126
Csp6I	GTAC	2 cut(s)	618, 801
CspCI	CAANNNNNGTGG	2 cut(s)	496, 531
CviAII	CATG	3 cut(s)	220, 370, 688
CviQI	GTAC	2 cut(s)	618, 801
DdeI	CTNAG	3 cut(s)	50, 489, 820
DpnI	GATC	8 cut(s)	13, 37, 55, 70, 81, 295, 327, 564
DpnII	GATC	8 cut(s)	11, 35, 53, 68, 79, 293, 325, 562
DrdI	GACNNNNNNGTC	1 cut(s)	182
DseDI	GACNNNNNNGTC	1 cut(s)	182
EaeI	YGGCCR	1 cut(s)	771
Eam1104I	CTCTTC	1 cut(s)	566
EarI	CTCTTC	1 cut(s)	566
EciI	GGCGGA	1 cut(s)	286
Eco130I	CCWWGG	1 cut(s)	525
Eco24I	GRGCYC	1 cut(s)	279
Eco31I	GGTCTC	1 cut(s)	122
Eco47I	GGWCC	1 cut(s)	447
EcoRII	CCWGG	2 cut(s)	281, 809
EcoT14I	CCWWGG	1 cut(s)	525
EcoT38I	GRGCYC	1 cut(s)	279
ErhI	CCWWGG	1 cut(s)	525
FaeI	CATG	3 cut(s)	223, 373, 691
FaiI	YATR	7 cut(s)	221, 312, 371, 396, 689, 746, 827
FaqI	GGGAC	1 cut(s)	499
FatI	CATG	3 cut(s)	219, 369, 687
FbaI	TGATCA	1 cut(s)	562
FblI	GTMKAC	1 cut(s)	186
FokI	GGATG	1 cut(s)	241
FriOI	GRGCYC	1 cut(s)	279
FspBI	CTAG	2 cut(s)	608, 615
GlaI	GCGC	1 cut(s)	98
GsaI	CCCAGC	1 cut(s)	728
GsuI	CTGGAG	2 cut(s)	193, 793
HaeIII	GGCC	3 cut(s)	286, 505, 773
HapII	CCGG	1 cut(s)	684
HgaI	GACGC	2 cut(s)	109, 126
HhaI	GCGC	1 cut(s)	99
Hin1I	GRCGYC	1 cut(s)	183
Hin1II	CATG	3 cut(s)	223, 373, 691
Hin6I	GCGC	1 cut(s)	97
HinP1I	GCGC	1 cut(s)	97
HincII	GTYRAC	1 cut(s)	187
HindII	GTYRAC	1 cut(s)	187
HinfI	GANTC	3 cut(s)	41, 408, 765
HpaII	CCGG	1 cut(s)	684
HphI	GGTGA	1 cut(s)	566
Hpy166II	GTNNAC	3 cut(s)	187, 407, 535
Hpy188I	TCNGA	9 cut(s)	16, 40, 51, 73, 116, 136, 175, 293, 330
Hpy188III	TCNNGA	4 cut(s)	22, 210, 346, 758
Hpy8I	GTNNAC	3 cut(s)	187, 407, 535
Hpy99I	CGWCG	7 cut(s)	120, 125, 185, 188, 191, 251, 320
HpyAV	CCTTC	3 cut(s)	131, 185, 452
HpyCH4III	ACNGT	2 cut(s)	320, 622
HpyCH4IV	ACGT	3 cut(s)	183, 403, 732
HpyCH4V	TGCA	5 cut(s)	33, 240, 379, 535, 816
HpyF10VI	GCNNNNNNNGC	7 cut(s)	90, 283, 499, 502, 643, 677, 813
HpyF3I	CTNAG	3 cut(s)	50, 489, 820
HpySE526I	ACGT	3 cut(s)	183, 403, 732
Hsp92I	GRCGYC	1 cut(s)	183
Hsp92II	CATG	3 cut(s)	223, 373, 691
HspAI	GCGC	1 cut(s)	97
KpnI	GGTACC	1 cut(s)	804
Ksp22I	TGATCA	1 cut(s)	562
KspI	CCGCGG	2 cut(s)	235, 273
Kzo9I	GATC	8 cut(s)	11, 35, 53, 68, 79, 293, 325, 562
LguI	GCTCTTC	1 cut(s)	566
LmnI	GCTCC	3 cut(s)	212, 274, 812
Lsp1109I	GCAGC	5 cut(s)	224, 480, 535, 615, 658
LweI	GCATC	3 cut(s)	159, 372, 825
MaeI	CTAG	2 cut(s)	608, 615
MaeII	ACGT	3 cut(s)	183, 403, 732
MaeIII	GTNAC	1 cut(s)	554
MalI	GATC	8 cut(s)	13, 37, 55, 70, 81, 295, 327, 564
MboI	GATC	8 cut(s)	11, 35, 53, 68, 79, 293, 325, 562
MboII	GAAGA	8 cut(s)	145, 397, 437, 583, 638, 706, 709, 762
MflI	RGATCY	5 cut(s)	11, 35, 68, 293, 325
MhlI	GDGCHC	2 cut(s)	279, 537
MluCI	AATT	3 cut(s)	374, 440, 660
MluI	ACGCGT	1 cut(s)	118
MlyI	GAGTC	1 cut(s)	402
MmeI	TCCRAC	2 cut(s)	139, 198
MslI	CAYNNNNRTG	3 cut(s)	368, 540, 686
MspA1I	CMGCKG	2 cut(s)	234, 272
MspI	CCGG	1 cut(s)	684
MspR9I	CCNGG	2 cut(s)	283, 811
MvaI	CCWGG	2 cut(s)	283, 811
MvnI	CGCG	3 cut(s)	120, 234, 272
MwoI	GCNNNNNNNGC	7 cut(s)	90, 283, 499, 502, 643, 677, 813
NdeII	GATC	8 cut(s)	11, 35, 53, 68, 79, 293, 325, 562
NlaIII	CATG	3 cut(s)	223, 373, 691
NlaIV	GGNNCC	3 cut(s)	208, 276, 802
NmeAIII	GCCGAG	1 cut(s)	390
NmuCI	GTSAC	1 cut(s)	554
OliI	CACNNNNGTG	1 cut(s)	540
PciSI	GCTCTTC	1 cut(s)	566
PcsI	WCGNNNNNNNCGW	3 cut(s)	186, 223, 417
PfeI	GAWTC	2 cut(s)	41, 765
Ple19I	CGATCG	1 cut(s)	82
PleI	GAGTC	1 cut(s)	402
PpsI	GAGTC	1 cut(s)	402
Psp6I	CCWGG	2 cut(s)	281, 809
PspFI	CCCAGC	1 cut(s)	724
PspGI	CCWGG	2 cut(s)	281, 809
PspN4I	GGNNCC	3 cut(s)	208, 276, 802
PspPI	GGNCC	3 cut(s)	285, 447, 504
PstI	CTGCAG	1 cut(s)	35
PsuI	RGATCY	5 cut(s)	11, 35, 68, 293, 325
PvuI	CGATCG	1 cut(s)	82
RsaI	GTAC	2 cut(s)	619, 802
RsaNI	GTAC	2 cut(s)	618, 801
RseI	CAYNNNNRTG	3 cut(s)	368, 540, 686
SacII	CCGCGG	2 cut(s)	235, 273
SalI	GTCGAC	1 cut(s)	185
SapI	GCTCTTC	1 cut(s)	566
Sau3AI	GATC	8 cut(s)	11, 35, 53, 68, 79, 293, 325, 562
Sau96I	GGNCC	3 cut(s)	285, 447, 504
SchI	GAGTC	1 cut(s)	402
ScrFI	CCNGG	2 cut(s)	283, 811
SduI	GDGCHC	2 cut(s)	279, 537
SfaNI	GCATC	3 cut(s)	159, 372, 825
SfcI	CTRYAG	1 cut(s)	31
Sfr303I	CCGCGG	2 cut(s)	235, 273
SgrBI	CCGCGG	2 cut(s)	235, 273
SgrDI	CGTCGACG	1 cut(s)	185
SinI	GGWCC	1 cut(s)	447
SmiMI	CAYNNNNRTG	3 cut(s)	368, 540, 686
SmlI	CTYRAG	1 cut(s)	787
SmoI	CTYRAG	1 cut(s)	787
SpeI	ACTAGT	1 cut(s)	614
Sse9I	AATT	3 cut(s)	374, 440, 660
SspI	AATATT	1 cut(s)	334
SspMI	CTAG	2 cut(s)	608, 615
StyD4I	CCNGG	2 cut(s)	281, 809
StyI	CCWWGG	1 cut(s)	525
TaaI	ACNGT	2 cut(s)	320, 622
TaiI	ACGT	3 cut(s)	186, 406, 735
TaqI	TCGA	6 cut(s)	21, 56, 78, 186, 345, 717
TasI	AATT	3 cut(s)	374, 440, 660
TatI	WGTACW	1 cut(s)	617
TauI	GCSGC	9 cut(s)	207, 237, 272, 499, 502, 505, 674, 677, 680
TfiI	GAWTC	2 cut(s)	41, 765
TscAI	CASTG	2 cut(s)	627, 673
TseFI	GTSAC	1 cut(s)	554
TseI	GCWGC	5 cut(s)	237, 493, 548, 603, 646
Tsp45I	GTSAC	1 cut(s)	554
TspDTI	ATGAA	4 cut(s)	243, 386, 398, 471
TspRI	CASTG	2 cut(s)	627, 673
VneI	GTGCAC	1 cut(s)	533
VpaK11BI	GGWCC	1 cut(s)	447
XmiI	GTMKAC	1 cut(s)	186
XspI	CTAG	2 cut(s)	608, 615
ZraI	GACGTC	1 cut(s)	184

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.