Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001067

Physical Location & Seq

Genomic Coordinates Reverse (-)

1827922 .. 1830469

Gene Structure 2548 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G06060.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 981 bp

ATGGCGGCCATTTCTCCTCTCTTCCTTCTCTTCCTCATTCCCATTATCCCACTATCGCTTCTCGCTATCTTAGCCCTAATTGTACGTCCTCGTCCCATTAAGATCCCTATCAAGTCCCGTCACGCCTTCATCACCGGTGGATCTAGCGGCATCGGTCTTGCTCTCGCCCACCGCGCCGCTTCCGAGGGCGCTCGTGTCTCAATCCTCGCCCGATCCGGATCAAAGCTCGAGGAGGCTAAGAAATCGATCCAGCTCGCCACTGGTGTCGAAGTCGCCACATTCTCTGCAGACGTTCGCGATTACGATGCAGTCTCGAAGGCGATAGATGAATCGGGACCGATCGATGTGTTGATTGTTAACCAAGGTGTGTTTACGGCGAAGGAGCTGGTGAAACATAGTCCTGAAGATGTCAAATTCACGATTGACGTAAATCTTGTTGGGAGCTTCAATGTGATTAAAGCCGCTTTGCCTGCCATGAAAGCAAGGAAAGATCGTGGACCTGCTTCCATCAGTCTCGTCTCCTCTCAAGCTGGTCAGGTTGGTGTCTATGGTTATGCTGCATATTCAGCAAGCAAGTTTGGTCTTCAGGGTTTAGCGCAAGCATTGCAACAGGAAGTTATATCTGATGATATTCATGTCACTCTTATTTTTCCTCCTGACACTAATACACCCGGCTTTGAAGAAGAACAGAAGAGCAGACCTGAAGTCACTGCCATAATAGCTGCATCCTCTGGTTCAATGGAAACAGAAGAAGTAGCAAAAAAGGCTATGGATGGCATAAAAGCAGGAAACTTCACCGTCTCATGCAACTTTGAGGGATTCTTACTGTCTCTCGCGACAACCGGCATGTCCCCTCAAAGATCATTTTGGCTTGCTTTTCTTGAAGTTATAACCGCAGGGCCTATAAGATTAATCGCTCTCTTCTTTCAATGGGATTGGTATAAAGCCATTGAAAAATGGAGCAAAACCAAAACCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006629 GO:0006643 GO:0006665 GO:0006666 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0012505 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0030148 GO:0031984 GO:0042175 GO:0042180 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044421 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046467 GO:0046519 GO:0047560 GO:0055114 GO:0071704 GO:0097164 GO:0098827 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00600 ko01100 map00600 map01100

Pfam Domains

Protein Families

adh_short

Protein Analysis

326

Amino Acids

34.98

Weight (kDa)

8.81

Isoelectric Point (pI)

35.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
adh_short	PF00106	58 - 248	7.2e-45	short chain dehydrogenase
KR	PF08659	60 - 221	1.7e-12	KR domain
Epimerase	PF01370	60 - 174	1.1e-06	NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd	PF16363	60 - 175	5e-06	GDP-mannose 4,6 dehydratase
adh_short_C2	PF13561	66 - 271	2.2e-27	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001067)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06060 AT3G06060 AT5G19200
fragaria_vesca	FvH4_4g14820
malus_domestica	MD13G1185700.v1.1
prunus_persica	Prupe.6G171900_v2.0.a1 Prupe.6G197600_v2.0.a1 Prupe.6G197600_v2.0.a1 Prupe.6G197600_v2.0.a1
pyrus_communis	pycom13g16020 pycom16g15630
rosa_chinensis	RchiOBHm_Chr4g0417501 RchiOBHm_Chr6g0272751 RchiOBHm_Chr6g0272831 RchiOBHm_Chr6g0272851 RchiOBHm_Chr6g0272861 RchiOBHm_Chr6g0272881
rosa_laevigata	RLG00000007902 RLG00000007903 RLG00000007908 RLG00000007909 RLG00000013658 RLG00000013659 RLG00000023098
rosa_multiflora	Rmu_co8170416.1_g000001 Rmu_co8391473.1_g000001 Rmu_sc0002793.1_g000014 Rmu_sc0004142.1_g000014 Rmu_sc0004142.1_g000018 Rmu_sc0004540.1_g000023 Rmu_ssc0000024.1_g000001
rosa_roxburghii	Rroxscaffold_7G00195980 Rroxscaffold_7G00196010 Rroxscaffold_7G00196020
rosa_rugosa	Rorug04G0148100 Rorug04G0148100 Rorug04G0148200 Rorug04G0148300 Rorug04G0148400 Rorug06G0070800
rosa_samantha	Rh4AG211400 Rh4BG211600 Rh4CG222100 Rh4DG209100 Rh6AG186800 Rh6BG190200 Rh6CG189000 Rh6CG189300 Rh6CG189600 Rh6DG180400 Rh6DG180700 Rh6DG180800 Rh6DG180900 Rh7AG364400 Rh7DG358900
rosa_wichuraiana	Rw4G017870 Rw6G016120 Rw6G016130 Rw6G016150 Rw7G030890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	890
Acc36I	ACCTGC	1 cut(s)	508
AccII	CGCG	3 cut(s)	174, 297, 836
AccIII	TCCGGA	1 cut(s)	215
AciI	CCGC	6 cut(s)	5, 147, 172, 177, 462, 894
AclWI	GGATC	5 cut(s)	97, 148, 207, 226, 241
AcoI	YGGCCR	1 cut(s)	6
AcsI	RAATTY	1 cut(s)	413
AcuI	CTGAAG	3 cut(s)	423, 569, 723
AfaI	GTAC	1 cut(s)	84
AgeI	ACCGGT	1 cut(s)	134
AgsI	TTSAA	6 cut(s)	448, 680, 738, 884, 929, 953
AhdI	GACNNNNNGTC	1 cut(s)	704
AluBI	AGCT	6 cut(s)	226, 253, 385, 444, 530, 722
AluI	AGCT	6 cut(s)	226, 253, 385, 444, 530, 722
Alw26I	GTCTC	6 cut(s)	202, 316, 518, 523, 805, 834
AlwI	GGATC	5 cut(s)	97, 148, 207, 226, 241
Ama87I	CYCGRG	1 cut(s)	227
Aor13HI	TCCGGA	1 cut(s)	215
AoxI	GGCC	2 cut(s)	6, 899
ApeKI	GCWGC	2 cut(s)	557, 722
ApoI	RAATTY	1 cut(s)	413
AseI	ATTAAT	1 cut(s)	911
AsiGI	ACCGGT	1 cut(s)	134
AspLEI	GCGC	3 cut(s)	176, 191, 598
AspS9I	GGNCC	3 cut(s)	335, 497, 899
AsuC2I	CCSGG	1 cut(s)	672
AsuHPI	GGTGA	3 cut(s)	124, 400, 787
AvaI	CYCGRG	1 cut(s)	227
AvaII	GGWCC	2 cut(s)	335, 497
BauI	CACGAG	1 cut(s)	192
BbsI	GAAGAC	1 cut(s)	575
BbvI	GCAGC	2 cut(s)	544, 709
BccI	CCATC	2 cut(s)	515, 767
BceAI	ACGGC	1 cut(s)	390
BcgI	CGANNNNNNTGC	2 cut(s)	287, 321
BcnI	CCSGG	1 cut(s)	672
BcoDI	GTCTC	6 cut(s)	202, 316, 518, 523, 805, 834
BfaI	CTAG	1 cut(s)	144
BfmI	CTRYAG	1 cut(s)	285
BfoI	RGCGCY	1 cut(s)	192
BfuAI	ACCTGC	1 cut(s)	508
BisI	GCNGC	6 cut(s)	6, 148, 177, 462, 558, 723
BlsI	GCNGC	6 cut(s)	7, 149, 178, 463, 559, 724
Bme1390I	CCNGG	1 cut(s)	672
Bme18I	GGWCC	2 cut(s)	335, 497
BmeRI	GACNNNNNGTC	1 cut(s)	704
BmeT110I	CYCGRG	1 cut(s)	227
BmgT120I	GGNCC	3 cut(s)	335, 497, 899
BmiI	GGNNCC	1 cut(s)	336
BmrFI	CCNGG	1 cut(s)	672
BmsI	GCATC	3 cut(s)	159, 295, 734
BpiI	GAAGAC	1 cut(s)	575
BpuEI	CTTGAG	1 cut(s)	510
BpuMI	CCSGG	1 cut(s)	672
Bsa29I	ATCGAT	2 cut(s)	245, 342
BsaBI	GATNNNNATC	2 cut(s)	107, 217
BsaJI	CCNNGG	2 cut(s)	183, 361
BsaWI	WCCGGW	2 cut(s)	134, 215
Bse118I	RCCGGY	2 cut(s)	134, 842
Bse1I	ACTGG	1 cut(s)	265
Bse3DI	GCAATG	1 cut(s)	602
Bse8I	GATNNNNATC	2 cut(s)	107, 217
BseAI	TCCGGA	1 cut(s)	215
BseCI	ATCGAT	2 cut(s)	245, 342
BseDI	CCNNGG	2 cut(s)	183, 361
BseGI	GGATG	2 cut(s)	725, 778
BseJI	GATNNNNATC	2 cut(s)	107, 217
BseMI	GCAATG	1 cut(s)	602
BseNI	ACTGG	1 cut(s)	265
BseRI	GAGGAG	3 cut(s)	6, 245, 511
BseXI	GCAGC	2 cut(s)	544, 709
Bsh1236I	CGCG	3 cut(s)	174, 297, 836
Bsh1285I	CGRYCG	1 cut(s)	342
BshFI	GGCC	2 cut(s)	8, 901
BshTI	ACCGGT	1 cut(s)	134
BshVI	ATCGAT	2 cut(s)	245, 342
BsiEI	CGRYCG	1 cut(s)	342
BsiHKCI	CYCGRG	1 cut(s)	227
BsiSI	CCGG	4 cut(s)	135, 216, 672, 843
BslFI	GGGAC	4 cut(s)	78, 100, 348, 835
BsmAI	GTCTC	6 cut(s)	202, 316, 518, 523, 805, 834
BsmBI	CGTCTC	2 cut(s)	523, 805
BsmFI	GGGAC	4 cut(s)	78, 100, 348, 835
BsnI	GGCC	2 cut(s)	8, 901
BsoBI	CYCGRG	1 cut(s)	227
Bsp13I	TCCGGA	1 cut(s)	215
Bsp143I	GATC	8 cut(s)	102, 140, 212, 218, 246, 339, 490, 860
Bsp68I	TCGCGA	2 cut(s)	297, 836
BspACI	CCGC	6 cut(s)	5, 147, 172, 177, 462, 894
BspANI	GGCC	2 cut(s)	8, 901
BspDI	ATCGAT	2 cut(s)	245, 342
BspEI	TCCGGA	1 cut(s)	215
BspFNI	CGCG	3 cut(s)	174, 297, 836
BspLI	GGNNCC	1 cut(s)	336
BspMAI	CTGCAG	1 cut(s)	289
BspMI	ACCTGC	1 cut(s)	508
BspPI	GGATC	5 cut(s)	97, 148, 207, 226, 241
BspQI	GCTCTTC	1 cut(s)	686
BsrDI	GCAATG	1 cut(s)	602
BsrFI	RCCGGY	2 cut(s)	134, 842
BsrI	ACTGG	1 cut(s)	265
BssAI	RCCGGY	2 cut(s)	134, 842
BssECI	CCNNGG	2 cut(s)	183, 361
BssMI	GATC	8 cut(s)	102, 140, 212, 218, 246, 339, 490, 860
BssSI	CACGAG	1 cut(s)	192
BssT1I	CCWWGG	1 cut(s)	361
Bst2BI	CACGAG	1 cut(s)	192
Bst4CI	ACNGT	2 cut(s)	799, 828
Bst6I	CTCTTC	4 cut(s)	26, 35, 686, 926
BstAPI	GCANNNNNTGC	1 cut(s)	604
BstC8I	GCNNGC	5 cut(s)	255, 471, 571, 600, 873
BstDEI	CTNAG	2 cut(s)	70, 237
BstF5I	GGATG	2 cut(s)	725, 778
BstFNI	CGCG	3 cut(s)	174, 297, 836
BstH2I	RGCGCY	1 cut(s)	192
BstHHI	GCGC	3 cut(s)	176, 191, 598
BstKTI	GATC	8 cut(s)	105, 143, 215, 221, 249, 342, 493, 863
BstMAI	GTCTC	6 cut(s)	202, 316, 518, 523, 805, 834
BstMBI	GATC	8 cut(s)	102, 140, 212, 218, 246, 339, 490, 860
BstMCI	CGRYCG	1 cut(s)	342
BstMWI	GCNNNNNNNGC	8 cut(s)	71, 173, 470, 479, 566, 604, 719, 764
BstNSI	RCATGY	1 cut(s)	850
BstSCI	CCNGG	1 cut(s)	670
BstSFI	CTRYAG	1 cut(s)	285
BstUI	CGCG	3 cut(s)	174, 297, 836
BstV1I	GCAGC	2 cut(s)	544, 709
BstV2I	GAAGAC	1 cut(s)	575
BstX2I	RGATCY	2 cut(s)	102, 140
BstYI	RGATCY	2 cut(s)	102, 140
Bsu15I	ATCGAT	2 cut(s)	245, 342
BsuRI	GGCC	2 cut(s)	8, 901
BsuTUI	ATCGAT	2 cut(s)	245, 342
BtsCI	GGATG	2 cut(s)	725, 778
BtsI	GCAGTG	1 cut(s)	708
BtsIMutI	CAGTG	2 cut(s)	258, 708
BtuMI	TCGCGA	2 cut(s)	297, 836
BveI	ACCTGC	1 cut(s)	508
Cac8I	GCNNGC	5 cut(s)	255, 471, 571, 600, 873
CfoI	GCGC	3 cut(s)	176, 191, 598
Cfr10I	RCCGGY	2 cut(s)	134, 842
Cfr13I	GGNCC	3 cut(s)	335, 497, 899
ClaI	ATCGAT	2 cut(s)	245, 342
Csp6I	GTAC	1 cut(s)	83
CspAI	ACCGGT	1 cut(s)	134
CviAII	CATG	4 cut(s)	475, 635, 804, 847
CviQI	GTAC	1 cut(s)	83
DdeI	CTNAG	2 cut(s)	70, 237
DpnI	GATC	8 cut(s)	104, 142, 214, 220, 248, 341, 492, 862
DpnII	GATC	8 cut(s)	102, 140, 212, 218, 246, 339, 490, 860
DriI	GACNNNNNGTC	1 cut(s)	704
EaeI	YGGCCR	1 cut(s)	6
Eam1104I	CTCTTC	4 cut(s)	26, 35, 686, 926
Eam1105I	GACNNNNNGTC	1 cut(s)	704
EarI	CTCTTC	4 cut(s)	26, 35, 686, 926
Eco130I	CCWWGG	1 cut(s)	361
Eco47I	GGWCC	2 cut(s)	335, 497
Eco57I	CTGAAG	3 cut(s)	423, 569, 723
Eco88I	CYCGRG	1 cut(s)	227
EcoO109I	RGGNCCY	1 cut(s)	899
EcoT14I	CCWWGG	1 cut(s)	361
ErhI	CCWWGG	1 cut(s)	361
Esp3I	CGTCTC	2 cut(s)	523, 805
FaeI	CATG	4 cut(s)	478, 638, 807, 850
FaqI	GGGAC	4 cut(s)	78, 100, 348, 835
FatI	CATG	4 cut(s)	474, 634, 803, 846
Fnu4HI	GCNGC	6 cut(s)	6, 148, 177, 462, 558, 723
FokI	GGATG	2 cut(s)	712, 785
Fsp4HI	GCNGC	6 cut(s)	6, 148, 177, 462, 558, 723
FspBI	CTAG	1 cut(s)	144
GlaI	GCGC	3 cut(s)	175, 190, 597
GluI	GCNGC	6 cut(s)	6, 148, 177, 462, 558, 723
HaeII	RGCGCY	1 cut(s)	192
HaeIII	GGCC	2 cut(s)	8, 901
HapII	CCGG	4 cut(s)	135, 216, 672, 843
HhaI	GCGC	3 cut(s)	176, 191, 598
Hin1II	CATG	4 cut(s)	478, 638, 807, 850
Hin6I	GCGC	3 cut(s)	174, 189, 596
HinP1I	GCGC	3 cut(s)	174, 189, 596
HincII	GTYRAC	1 cut(s)	358
HindII	GTYRAC	1 cut(s)	358
HinfI	GANTC	2 cut(s)	329, 819
HpaI	GTTAAC	1 cut(s)	358
HpaII	CCGG	4 cut(s)	135, 216, 672, 843
HphI	GGTGA	3 cut(s)	124, 400, 787
Hpy166II	GTNNAC	3 cut(s)	358, 372, 497
Hpy188I	TCNGA	2 cut(s)	184, 625
Hpy188III	TCNNGA	9 cut(s)	216, 296, 313, 333, 401, 418, 656, 835, 881
Hpy8I	GTNNAC	3 cut(s)	358, 372, 497
HpyAV	CCTTC	4 cut(s)	35, 136, 310, 373
HpyCH4III	ACNGT	2 cut(s)	799, 828
HpyCH4IV	ACGT	3 cut(s)	85, 291, 426
HpyCH4V	TGCA	6 cut(s)	287, 308, 560, 607, 725, 807
HpyF10VI	GCNNNNNNNGC	8 cut(s)	71, 173, 470, 479, 566, 604, 719, 764
HpyF3I	CTNAG	2 cut(s)	70, 237
HpySE526I	ACGT	3 cut(s)	85, 291, 426
Hsp92II	CATG	4 cut(s)	478, 638, 807, 850
HspAI	GCGC	3 cut(s)	174, 189, 596
Kpn2I	TCCGGA	1 cut(s)	215
KspAI	GTTAAC	1 cut(s)	358
Kzo9I	GATC	8 cut(s)	102, 140, 212, 218, 246, 339, 490, 860
LguI	GCTCTTC	1 cut(s)	686
LmnI	GCTCC	3 cut(s)	382, 441, 960
Lsp1109I	GCAGC	2 cut(s)	544, 709
LweI	GCATC	3 cut(s)	159, 295, 734
MaeI	CTAG	1 cut(s)	144
MaeII	ACGT	3 cut(s)	85, 291, 426
MaeIII	GTNAC	3 cut(s)	119, 637, 706
MalI	GATC	8 cut(s)	104, 142, 214, 220, 248, 341, 492, 862
MboI	GATC	8 cut(s)	102, 140, 212, 218, 246, 339, 490, 860
MboII	GAAGA	9 cut(s)	13, 22, 416, 575, 692, 695, 703, 761, 913
MflI	RGATCY	2 cut(s)	102, 140
MluCI	AATT	2 cut(s)	78, 413
MroI	TCCGGA	1 cut(s)	215
MseI	TTAA	4 cut(s)	99, 357, 456, 911
MspI	CCGG	4 cut(s)	135, 216, 672, 843
MspR9I	CCNGG	1 cut(s)	672
MvnI	CGCG	3 cut(s)	174, 297, 836
MwoI	GCNNNNNNNGC	8 cut(s)	71, 173, 470, 479, 566, 604, 719, 764
NciI	CCSGG	1 cut(s)	672
NdeII	GATC	8 cut(s)	102, 140, 212, 218, 246, 339, 490, 860
NlaIII	CATG	4 cut(s)	478, 638, 807, 850
NlaIV	GGNNCC	1 cut(s)	336
NmuCI	GTSAC	3 cut(s)	119, 637, 706
NruI	TCGCGA	2 cut(s)	297, 836
NspI	RCATGY	1 cut(s)	850
PaeR7I	CTCGAG	1 cut(s)	227
PciSI	GCTCTTC	1 cut(s)	686
PfeI	GAWTC	2 cut(s)	329, 819
PinAI	ACCGGT	1 cut(s)	134
PkrI	GCNGC	6 cut(s)	7, 149, 178, 463, 559, 724
Ple19I	CGATCG	1 cut(s)	342
PshBI	ATTAAT	1 cut(s)	911
PsiI	TTATAA	1 cut(s)	890
PspN4I	GGNNCC	1 cut(s)	336
PspPI	GGNCC	3 cut(s)	335, 497, 899
PspXI	VCTCGAGB	1 cut(s)	227
PstI	CTGCAG	1 cut(s)	289
PsuI	RGATCY	2 cut(s)	102, 140
PvuI	CGATCG	1 cut(s)	342
RruI	TCGCGA	2 cut(s)	297, 836
RsaI	GTAC	1 cut(s)	84
RsaNI	GTAC	1 cut(s)	83
SapI	GCTCTTC	1 cut(s)	686
SaqAI	TTAA	4 cut(s)	99, 357, 456, 911
SatI	GCNGC	6 cut(s)	6, 148, 177, 462, 558, 723
Sau3AI	GATC	8 cut(s)	102, 140, 212, 218, 246, 339, 490, 860
Sau96I	GGNCC	3 cut(s)	335, 497, 899
ScrFI	CCNGG	1 cut(s)	672
SfaNI	GCATC	3 cut(s)	159, 295, 734
SfcI	CTRYAG	1 cut(s)	285
Sfr274I	CTCGAG	1 cut(s)	227
SgrAI	CRCCGGYG	1 cut(s)	134
SinI	GGWCC	2 cut(s)	335, 497
SlaI	CTCGAG	1 cut(s)	227
SmlI	CTYRAG	2 cut(s)	227, 525
SmoI	CTYRAG	2 cut(s)	227, 525
Sse9I	AATT	2 cut(s)	78, 413
SsiI	CCGC	6 cut(s)	5, 147, 172, 177, 462, 894
SspMI	CTAG	1 cut(s)	144
StyD4I	CCNGG	1 cut(s)	670
StyI	CCWWGG	1 cut(s)	361
TaaI	ACNGT	2 cut(s)	799, 828
TaiI	ACGT	3 cut(s)	88, 294, 429
TaqI	TCGA	5 cut(s)	228, 245, 267, 314, 342
TaqII	GACCGA	2 cut(s)	143, 352
TasI	AATT	2 cut(s)	78, 413
TauI	GCSGC	4 cut(s)	8, 150, 179, 464
TfiI	GAWTC	2 cut(s)	329, 819
Tru1I	TTAA	4 cut(s)	99, 357, 456, 911
Tru9I	TTAA	4 cut(s)	99, 357, 456, 911
TscAI	CASTG	2 cut(s)	265, 715
TseFI	GTSAC	3 cut(s)	119, 637, 706
TseI	GCWGC	2 cut(s)	557, 722
Tsp45I	GTSAC	3 cut(s)	119, 637, 706
TspDTI	ATGAA	4 cut(s)	118, 342, 491, 623
TspRI	CASTG	2 cut(s)	265, 715
VpaK11BI	GGWCC	2 cut(s)	335, 497
VspI	ATTAAT	1 cut(s)	911
XapI	RAATTY	1 cut(s)	413
XceI	RCATGY	1 cut(s)	850
XcmI	CCANNNNNNNNNTGG	1 cut(s)	257
XhoI	CTCGAG	1 cut(s)	227
XspI	CTAG	1 cut(s)	144

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.