Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001067

Physical Location & Seq

Genomic Coordinates Forward (+)

15809800 .. 15811793

Gene Structure 1994 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1185700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGGTGGACCTCTCCTTCCTCGCCCTCCTCCTCCTCCTCCCGCTCGCCCTCCTCTTCCTCATTCTCTACGTCATAGTCCGGCCCTGCCCCGTCAATATCCCAATCAAGAACCGCCACGTGTTCATCACCGGAGGCTCCAGCGGAATCGGTCTCGCCCTGGCCCACCAGGCCGCCACGGCGGGCGCAAGAGTCTCCATCCTCGCGCGCTCCCTCGACAAGCTCGAGGAAGCAAAGAACTACATCCAGCTGTCCACCGGCATCGACGTGTCAATCTTCGCCGCCGACGTCCGCGACTACGACGCCGTTTCGAAGGCAGTGGAGGAGGCGGGTCCCATCGACTTGTTGATCGTGAACCAGGGGGTGTTCGTCGCCGAGGAGCTCGAGAAGCAGGGATTGGATGAGGTGAGGTTCATGGTGGACGTGAATCTGATTGGGAGCTTCAACATGATCAAAGCCGCTCTCCCCGAAATGAAGAAGGGAGAGGACCGTGGGCCCCGCTCGATCGCGCTGATGTCATCCCAGGCCGGCCAGGTCGGAATCTACGGCTACACGGCCTACTCCGCCAGCAAGTTCGGGCTCCGTGGCTTGGGCGAGGCTCTGCAGCAAGAGGTGATTTCTCACGACATTCACGTTTCTCTTGTATTCCCGCCGGACACAGACACCCCTGGTTTCGCTGAAGAGAACAAGAGGCGGCCGCCGCTGACTGGCATTATAGCGGAGTCGTCTGGGGCGATGAAAGCCGAAGATGTTGCGAAGAGGGCTTTGGATGGGATTAAGTCCGCTTCTTTTATTGTGCGTTGCAACTTTGAAGGGTTATTGTTGTCTATTGCCACTGCTGGGATGTCTCCTCAGAGGTCGTTCGTTATGGCATTTTTGGAGGTGGTTGCGGGTGGTTTGGTTCGTCTTGTTGCTCTGTGTTTTCAGTGGAGTTGGTATACCAGCATACACAAGTGGCAAGCACAGAGCAAGAGGACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006629 GO:0006643 GO:0006665 GO:0006666 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0012505 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0030148 GO:0031984 GO:0042175 GO:0042180 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044421 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046467 GO:0046519 GO:0047560 GO:0055114 GO:0071704 GO:0097164 GO:0098827 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00600 ko01100 map00600 map01100

Pfam Domains

Protein Families

adh_short

Protein Analysis

326

Amino Acids

35.17

Weight (kDa)

6.24

Isoelectric Point (pI)

37.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
adh_short	PF00106	39 - 230	1.4e-42	short chain dehydrogenase
KR	PF08659	40 - 190	3.8e-10	KR domain
adh_short_C2	PF13561	47 - 229	2.2e-24	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001067)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06060 AT3G06060 AT5G19200
fragaria_vesca	FvH4_4g14820
malus_domestica	MD13G1185700.v1.1
prunus_persica	Prupe.6G171900_v2.0.a1 Prupe.6G197600_v2.0.a1 Prupe.6G197600_v2.0.a1 Prupe.6G197600_v2.0.a1
pyrus_communis	pycom13g16020 pycom16g15630
rosa_chinensis	RchiOBHm_Chr4g0417501 RchiOBHm_Chr6g0272751 RchiOBHm_Chr6g0272831 RchiOBHm_Chr6g0272851 RchiOBHm_Chr6g0272861 RchiOBHm_Chr6g0272881
rosa_laevigata	RLG00000007902 RLG00000007903 RLG00000007908 RLG00000007909 RLG00000013658 RLG00000013659 RLG00000023098
rosa_multiflora	Rmu_co8170416.1_g000001 Rmu_co8391473.1_g000001 Rmu_sc0002793.1_g000014 Rmu_sc0004142.1_g000014 Rmu_sc0004142.1_g000018 Rmu_sc0004540.1_g000023 Rmu_ssc0000024.1_g000001
rosa_roxburghii	Rroxscaffold_7G00195980 Rroxscaffold_7G00196010 Rroxscaffold_7G00196020
rosa_rugosa	Rorug04G0148100 Rorug04G0148100 Rorug04G0148200 Rorug04G0148300 Rorug04G0148400 Rorug06G0070800
rosa_samantha	Rh4AG211400 Rh4BG211600 Rh4CG222100 Rh4DG209100 Rh6AG186800 Rh6BG190200 Rh6CG189000 Rh6CG189300 Rh6CG189600 Rh6DG180400 Rh6DG180700 Rh6DG180800 Rh6DG180900 Rh7AG364400 Rh7DG358900
rosa_wichuraiana	Rw4G017870 Rw6G016120 Rw6G016130 Rw6G016150 Rw7G030890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	288
AccBSI	CCGCTC	3 cut(s)	43, 458, 498
AccI	GTMKAC	1 cut(s)	935
AccII	CGCG	4 cut(s)	203, 205, 291, 506
AcoI	YGGCCR	2 cut(s)	526, 692
AcuI	CTGAAG	1 cut(s)	696
AcvI	CACGTG	1 cut(s)	118
AcyI	GRCGYC	2 cut(s)	285, 300
AfiI	CCNNNNNNNGG	3 cut(s)	586, 704, 837
AflIII	ACRYGT	2 cut(s)	117, 264
AgsI	TTSAA	2 cut(s)	442, 809
AjiI	CACGTC	2 cut(s)	265, 421
AjnI	CCWGG	6 cut(s)	156, 165, 354, 519, 528, 664
AjuI	GAANNNNNNNTTGG	4 cut(s)	377, 409, 746, 778
AluBI	AGCT	4 cut(s)	220, 247, 379, 438
AluI	AGCT	4 cut(s)	220, 247, 379, 438
Alw21I	GWGCWC	1 cut(s)	381
Alw26I	GTCTC	3 cut(s)	155, 196, 849
Ama87I	CYCGRG	2 cut(s)	221, 380
AoxI	GGCC	8 cut(s)	80, 159, 168, 491, 522, 526, 552, 692
ApaI	GGGCCC	1 cut(s)	495
ApeKI	GCWGC	1 cut(s)	601
AspLEI	GCGC	4 cut(s)	185, 205, 207, 508
AspS9I	GGNCC	8 cut(s)	7, 81, 160, 329, 484, 491, 492, 972
AsuHPI	GGTGA	3 cut(s)	118, 415, 622
AsuII	TTCGAA	1 cut(s)	308
AvaI	CYCGRG	2 cut(s)	221, 380
AvaII	GGWCC	4 cut(s)	7, 329, 484, 972
BaeGI	GKGCMC	1 cut(s)	495
BanII	GRGCYC	3 cut(s)	381, 495, 579
BbrPI	CACGTG	1 cut(s)	118
Bbv12I	GWGCWC	1 cut(s)	381
BbvI	GCAGC	1 cut(s)	613
BccI	CCATC	3 cut(s)	203, 341, 761
BceAI	ACGGC	4 cut(s)	192, 287, 559, 567
BcgI	CGANNNNNNTGC	2 cut(s)	731, 765
BciT130I	CCWGG	6 cut(s)	158, 167, 356, 521, 530, 666
BclI	TGATCA	1 cut(s)	447
BcoDI	GTCTC	3 cut(s)	155, 196, 849
BfmI	CTRYAG	1 cut(s)	599
BglI	GCCNNNNNGGC	2 cut(s)	167, 176
BisI	GCNGC	7 cut(s)	171, 279, 456, 602, 692, 695, 698
BlsI	GCNGC	7 cut(s)	172, 280, 457, 603, 693, 696, 699
Bme1390I	CCNGG	6 cut(s)	158, 167, 356, 521, 530, 666
Bme18I	GGWCC	4 cut(s)	7, 329, 484, 972
BmeT110I	CYCGRG	2 cut(s)	221, 380
BmgBI	CACGTC	2 cut(s)	265, 421
BmgT120I	GGNCC	8 cut(s)	7, 81, 160, 329, 484, 491, 492, 972
BmiI	GGNNCC	6 cut(s)	136, 330, 331, 493, 494, 578
BmrFI	CCNGG	6 cut(s)	158, 167, 356, 521, 530, 666
BmsI	GCATC	1 cut(s)	267
BpmI	CTGGAG	1 cut(s)	121
Bpu14I	TTCGAA	1 cut(s)	308
BsaAI	YACGTR	1 cut(s)	118
BsaHI	GRCGYC	2 cut(s)	285, 300
BsaI	GGTCTC	1 cut(s)	155
BsaJI	CCNNGG	8 cut(s)	156, 174, 355, 372, 487, 519, 580, 664
BsaWI	WCCGGW	1 cut(s)	128
Bsc4I	CCNNNNNNNGG	3 cut(s)	586, 704, 837
Bse118I	RCCGGY	2 cut(s)	254, 524
Bse1I	ACTGG	1 cut(s)	709
BseBI	CCWGG	6 cut(s)	158, 167, 356, 521, 530, 666
BseDI	CCNNGG	8 cut(s)	156, 174, 355, 372, 487, 519, 580, 664
BseGI	GGATG	6 cut(s)	195, 240, 403, 515, 772, 846
BseLI	CCNNNNNNNGG	3 cut(s)	586, 704, 837
BseMII	CTCAG	1 cut(s)	863
BseNI	ACTGG	1 cut(s)	709
BsePI	GCGCGC	1 cut(s)	203
BseRI	GAGGAG	8 cut(s)	17, 20, 23, 26, 41, 335, 389, 837
BseSI	GKGCMC	1 cut(s)	495
BseX3I	CGGCCG	1 cut(s)	692
BseXI	GCAGC	1 cut(s)	613
BseYI	CCCAGC	1 cut(s)	836
Bsh1236I	CGCG	4 cut(s)	203, 205, 291, 506
Bsh1285I	CGRYCG	2 cut(s)	504, 695
BshFI	GGCC	8 cut(s)	82, 161, 170, 493, 524, 528, 554, 694
BsiEI	CGRYCG	2 cut(s)	504, 695
BsiHKAI	GWGCWC	1 cut(s)	381
BsiHKCI	CYCGRG	2 cut(s)	221, 380
BsiSI	CCGG	5 cut(s)	79, 129, 255, 525, 650
BslFI	GGGAC	1 cut(s)	315
BslI	CCNNNNNNNGG	3 cut(s)	586, 704, 837
BsmAI	GTCTC	3 cut(s)	155, 196, 849
BsmFI	GGGAC	1 cut(s)	315
BsnI	GGCC	8 cut(s)	82, 161, 170, 493, 524, 528, 554, 694
Bso31I	GGTCTC	1 cut(s)	155
BsoBI	CYCGRG	2 cut(s)	221, 380
Bsp119I	TTCGAA	1 cut(s)	308
Bsp120I	GGGCCC	1 cut(s)	491
Bsp1286I	GDGCHC	3 cut(s)	381, 495, 579
Bsp143I	GATC	3 cut(s)	345, 447, 501
BspANI	GGCC	8 cut(s)	82, 161, 170, 493, 524, 528, 554, 694
BspCNI	CTCAG	1 cut(s)	862
BspFNI	CGCG	4 cut(s)	203, 205, 291, 506
BspLI	GGNNCC	6 cut(s)	136, 330, 331, 493, 494, 578
BspMAI	CTGCAG	1 cut(s)	603
BspT104I	TTCGAA	1 cut(s)	308
BspTNI	GGTCTC	1 cut(s)	155
BsrBI	CCGCTC	3 cut(s)	43, 458, 498
BsrFI	RCCGGY	2 cut(s)	254, 524
BsrI	ACTGG	1 cut(s)	709
BssAI	RCCGGY	2 cut(s)	254, 524
BssECI	CCNNGG	8 cut(s)	156, 174, 355, 372, 487, 519, 580, 664
BssHII	GCGCGC	1 cut(s)	203
BssMI	GATC	3 cut(s)	345, 447, 501
BssNAI	GTATAC	1 cut(s)	936
BssNI	GRCGYC	2 cut(s)	285, 300
Bst1107I	GTATAC	1 cut(s)	936
Bst2UI	CCWGG	6 cut(s)	158, 167, 356, 521, 530, 666
Bst4CI	ACNGT	1 cut(s)	488
Bst6I	CTCTTC	3 cut(s)	59, 672, 749
BstACI	GRCGYC	2 cut(s)	285, 300
BstBAI	YACGTR	1 cut(s)	118
BstBI	TTCGAA	1 cut(s)	308
BstC8I	GCNNGC	6 cut(s)	45, 181, 205, 526, 565, 957
BstDEI	CTNAG	1 cut(s)	849
BstDSI	CCRYGG	3 cut(s)	174, 487, 580
BstF5I	GGATG	6 cut(s)	195, 240, 403, 515, 772, 846
BstFNI	CGCG	4 cut(s)	203, 205, 291, 506
BstHHI	GCGC	4 cut(s)	185, 205, 207, 508
BstKTI	GATC	3 cut(s)	348, 450, 504
BstMAI	GTCTC	3 cut(s)	155, 196, 849
BstMBI	GATC	3 cut(s)	345, 447, 501
BstMCI	CGRYCG	2 cut(s)	504, 695
BstMWI	GCNNNNNNNGC	7 cut(s)	167, 176, 385, 560, 697, 737, 758
BstNI	CCWGG	6 cut(s)	158, 167, 356, 521, 530, 666
BstSCI	CCNGG	6 cut(s)	156, 165, 354, 519, 528, 664
BstSFI	CTRYAG	1 cut(s)	599
BstSLI	GKGCMC	1 cut(s)	495
BstUI	CGCG	4 cut(s)	203, 205, 291, 506
BstV1I	GCAGC	1 cut(s)	613
BstZ17I	GTATAC	1 cut(s)	936
BstZI	CGGCCG	1 cut(s)	692
BsuRI	GGCC	8 cut(s)	82, 161, 170, 493, 524, 528, 554, 694
BtgI	CCRYGG	3 cut(s)	174, 487, 580
BtgZI	GCGATG	1 cut(s)	746
BtrI	CACGTC	2 cut(s)	265, 421
BtsCI	GGATG	6 cut(s)	195, 240, 403, 515, 772, 846
BtsI	GCAGTG	2 cut(s)	321, 831
BtsIMutI	CAGTG	3 cut(s)	321, 831, 929
Cac8I	GCNNGC	6 cut(s)	45, 181, 205, 526, 565, 957
CciNI	GCGGCCGC	1 cut(s)	692
CfoI	GCGC	4 cut(s)	185, 205, 207, 508
Cfr10I	RCCGGY	2 cut(s)	254, 524
Cfr13I	GGNCC	8 cut(s)	7, 81, 160, 329, 484, 491, 492, 972
CseI	GACGC	1 cut(s)	308
CviAII	CATG	2 cut(s)	412, 445
DdeI	CTNAG	1 cut(s)	849
DpnI	GATC	3 cut(s)	347, 449, 503
DpnII	GATC	3 cut(s)	345, 447, 501
EaeI	YGGCCR	2 cut(s)	526, 692
EagI	CGGCCG	1 cut(s)	692
Eam1104I	CTCTTC	3 cut(s)	59, 672, 749
EarI	CTCTTC	3 cut(s)	59, 672, 749
EciI	GGCGGA	1 cut(s)	550
Ecl136II	GAGCTC	1 cut(s)	379
EclXI	CGGCCG	1 cut(s)	692
Eco24I	GRGCYC	3 cut(s)	381, 495, 579
Eco31I	GGTCTC	1 cut(s)	155
Eco47I	GGWCC	4 cut(s)	7, 329, 484, 972
Eco52I	CGGCCG	1 cut(s)	692
Eco53kI	GAGCTC	1 cut(s)	379
Eco57I	CTGAAG	1 cut(s)	696
Eco72I	CACGTG	1 cut(s)	118
Eco88I	CYCGRG	2 cut(s)	221, 380
EcoICRI	GAGCTC	1 cut(s)	379
EcoO109I	RGGNCCY	3 cut(s)	329, 492, 972
EcoRII	CCWGG	6 cut(s)	156, 165, 354, 519, 528, 664
EcoT38I	GRGCYC	3 cut(s)	381, 495, 579
FaeI	CATG	2 cut(s)	415, 448
FaiI	YATR	7 cut(s)	74, 413, 446, 713, 866, 936, 944
FaqI	GGGAC	1 cut(s)	315
FatI	CATG	2 cut(s)	411, 444
FauI	CCCGC	6 cut(s)	48, 172, 319, 503, 654, 880
FbaI	TGATCA	1 cut(s)	447
FblI	GTMKAC	1 cut(s)	935
Fnu4HI	GCNGC	7 cut(s)	171, 279, 456, 602, 692, 695, 698
FokI	GGATG	6 cut(s)	182, 227, 410, 502, 779, 853
FriOI	GRGCYC	3 cut(s)	381, 495, 579
FseI	GGCCGGCC	1 cut(s)	528
Fsp4HI	GCNGC	7 cut(s)	171, 279, 456, 602, 692, 695, 698
GlaI	GCGC	4 cut(s)	184, 204, 206, 507
GluI	GCNGC	7 cut(s)	171, 279, 456, 602, 692, 695, 698
GsaI	CCCAGC	1 cut(s)	840
GsuI	CTGGAG	1 cut(s)	121
HaeIII	GGCC	8 cut(s)	82, 161, 170, 493, 524, 528, 554, 694
HapII	CCGG	5 cut(s)	79, 129, 255, 525, 650
HgaI	GACGC	1 cut(s)	308
HhaI	GCGC	4 cut(s)	185, 205, 207, 508
Hin1I	GRCGYC	2 cut(s)	285, 300
Hin1II	CATG	2 cut(s)	415, 448
Hin6I	GCGC	4 cut(s)	183, 203, 205, 506
HinP1I	GCGC	4 cut(s)	183, 203, 205, 506
HinfI	GANTC	5 cut(s)	144, 189, 424, 537, 719
HpaII	CCGG	5 cut(s)	79, 129, 255, 525, 650
HphI	GGTGA	3 cut(s)	118, 415, 622
Hpy166II	GTNNAC	5 cut(s)	7, 252, 352, 418, 936
Hpy188I	TCNGA	3 cut(s)	429, 536, 852
Hpy188III	TCNNGA	4 cut(s)	106, 349, 382, 620
Hpy8I	GTNNAC	5 cut(s)	7, 252, 352, 418, 936
Hpy99I	CGWCG	4 cut(s)	266, 287, 302, 371
HpyAV	CCTTC	4 cut(s)	25, 304, 469, 803
HpyCH4III	ACNGT	1 cut(s)	488
HpyCH4IV	ACGT	6 cut(s)	69, 117, 264, 285, 420, 630
HpyCH4V	TGCA	2 cut(s)	601, 801
HpyF10VI	GCNNNNNNNGC	7 cut(s)	167, 176, 385, 560, 697, 737, 758
HpyF3I	CTNAG	1 cut(s)	849
HpySE526I	ACGT	6 cut(s)	69, 117, 264, 285, 420, 630
Hsp92I	GRCGYC	2 cut(s)	285, 300
Hsp92II	CATG	2 cut(s)	415, 448
HspAI	GCGC	4 cut(s)	183, 203, 205, 506
KflI	GGGWCCC	1 cut(s)	329
KroI	GCCGGC	1 cut(s)	524
KroNI	GCCGGC	1 cut(s)	526
Ksp22I	TGATCA	1 cut(s)	447
Kzo9I	GATC	3 cut(s)	345, 447, 501
LmnI	GCTCC	5 cut(s)	140, 212, 376, 435, 582
Lsp1109I	GCAGC	1 cut(s)	613
LweI	GCATC	1 cut(s)	267
MaeII	ACGT	6 cut(s)	69, 117, 264, 285, 420, 630
MalI	GATC	3 cut(s)	347, 449, 503
MbiI	CCGCTC	3 cut(s)	43, 458, 498
MboI	GATC	3 cut(s)	345, 447, 501
MboII	GAAGA	6 cut(s)	46, 265, 484, 689, 755, 766
MhlI	GDGCHC	3 cut(s)	381, 495, 579
MlyI	GAGTC	2 cut(s)	198, 728
MmeI	TCCRAC	1 cut(s)	514
MroNI	GCCGGC	1 cut(s)	524
MseI	TTAA	1 cut(s)	774
MslI	CAYNNNNRTG	1 cut(s)	263
MspA1I	CMGCKG	3 cut(s)	141, 247, 700
MspI	CCGG	5 cut(s)	79, 129, 255, 525, 650
MspR9I	CCNGG	6 cut(s)	158, 167, 356, 521, 530, 666
MvaI	CCWGG	6 cut(s)	158, 167, 356, 521, 530, 666
MvnI	CGCG	4 cut(s)	203, 205, 291, 506
MwoI	GCNNNNNNNGC	7 cut(s)	167, 176, 385, 560, 697, 737, 758
NaeI	GCCGGC	1 cut(s)	526
NdeII	GATC	3 cut(s)	345, 447, 501
NgoMIV	GCCGGC	1 cut(s)	524
NlaIII	CATG	2 cut(s)	415, 448
NlaIV	GGNNCC	6 cut(s)	136, 330, 331, 493, 494, 578
NmeAIII	GCCGAG	1 cut(s)	397
NotI	GCGGCCGC	1 cut(s)	692
NspV	TTCGAA	1 cut(s)	308
PaeR7I	CTCGAG	2 cut(s)	221, 380
PauI	GCGCGC	1 cut(s)	203
PcsI	WCGNNNNNNNCGW	4 cut(s)	219, 282, 627, 728
PdiI	GCCGGC	1 cut(s)	526
PfeI	GAWTC	3 cut(s)	144, 424, 537
PkrI	GCNGC	7 cut(s)	172, 280, 457, 603, 693, 696, 699
Ple19I	CGATCG	1 cut(s)	504
PleI	GAGTC	2 cut(s)	197, 727
PmaCI	CACGTG	1 cut(s)	118
PmlI	CACGTG	1 cut(s)	118
PpsI	GAGTC	2 cut(s)	197, 727
Ppu21I	YACGTR	1 cut(s)	118
PpuMI	RGGWCCY	2 cut(s)	329, 972
Psp124BI	GAGCTC	1 cut(s)	381
Psp5II	RGGWCCY	2 cut(s)	329, 972
Psp6I	CCWGG	6 cut(s)	156, 165, 354, 519, 528, 664
PspCI	CACGTG	1 cut(s)	118
PspFI	CCCAGC	1 cut(s)	836
PspGI	CCWGG	6 cut(s)	156, 165, 354, 519, 528, 664
PspN4I	GGNNCC	6 cut(s)	136, 330, 331, 493, 494, 578
PspOMI	GGGCCC	1 cut(s)	491
PspPI	GGNCC	8 cut(s)	7, 81, 160, 329, 484, 491, 492, 972
PspPPI	RGGWCCY	2 cut(s)	329, 972
PspXI	VCTCGAGB	1 cut(s)	221
PstI	CTGCAG	1 cut(s)	603
PteI	GCGCGC	1 cut(s)	203
PvuI	CGATCG	1 cut(s)	504
PvuII	CAGCTG	1 cut(s)	247
RigI	GGCCGGCC	1 cut(s)	528
RseI	CAYNNNNRTG	1 cut(s)	263
SacI	GAGCTC	1 cut(s)	381
SaqAI	TTAA	1 cut(s)	774
SatI	GCNGC	7 cut(s)	171, 279, 456, 602, 692, 695, 698
Sau3AI	GATC	3 cut(s)	345, 447, 501
Sau96I	GGNCC	8 cut(s)	7, 81, 160, 329, 484, 491, 492, 972
SchI	GAGTC	2 cut(s)	198, 728
ScrFI	CCNGG	6 cut(s)	158, 167, 356, 521, 530, 666
SduI	GDGCHC	3 cut(s)	381, 495, 579
SfaNI	GCATC	1 cut(s)	267
SfcI	CTRYAG	1 cut(s)	599
SfiI	GGCCNNNNNGGCC	1 cut(s)	167
Sfr274I	CTCGAG	2 cut(s)	221, 380
SfuI	TTCGAA	1 cut(s)	308
SinI	GGWCC	4 cut(s)	7, 329, 484, 972
SlaI	CTCGAG	2 cut(s)	221, 380
SmiMI	CAYNNNNRTG	1 cut(s)	263
SmlI	CTYRAG	2 cut(s)	221, 380
SmoI	CTYRAG	2 cut(s)	221, 380
SstI	GAGCTC	1 cut(s)	381
StyD4I	CCNGG	6 cut(s)	156, 165, 354, 519, 528, 664
TaaI	ACNGT	1 cut(s)	488
TaiI	ACGT	6 cut(s)	72, 120, 267, 288, 423, 633
TaqI	TCGA	7 cut(s)	213, 222, 261, 308, 336, 381, 500
TaqII	GACCGA	1 cut(s)	137
TauI	GCSGC	6 cut(s)	173, 281, 458, 694, 697, 700
TfiI	GAWTC	3 cut(s)	144, 424, 537
Tru1I	TTAA	1 cut(s)	774
Tru9I	TTAA	1 cut(s)	774
TscAI	CASTG	3 cut(s)	321, 838, 929
TseI	GCWGC	1 cut(s)	601
TspDTI	ATGAA	4 cut(s)	112, 400, 485, 749
TspGWI	ACGGA	1 cut(s)	569
TspRI	CASTG	3 cut(s)	321, 838, 929
VpaK11BI	GGWCC	4 cut(s)	7, 329, 484, 972
XhoI	CTCGAG	2 cut(s)	221, 380
XmiI	GTMKAC	1 cut(s)	935
ZraI	GACGTC	1 cut(s)	286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.