AT3G57520

galactinol--sucrose galactosyltransferase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000881
Physical Location & Seq
Reverse (-)
21288742 .. 21293171
4430 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G57520.1

Sequence Viewer

Length: 2322 bp
ATGACGATTACATCAAATATCTCTGTACAGAACGATAACCTCGTTGTTCAAGGGAAGACTATCCTTACCAAAATCCCTGACAACATTATCCTAACTCCGGTTACCGGTAATGGATTCGTTTCCGGTTCATTTATAGGCGCGACGTTTGAACAAAGCAAGAGTCTTCATGTGTTCCCCATCGGTGTATTGGAGGGTCTTCGGTTCATGTGCTGTTTCCGGTTTAAGCTATGGTGGATGACTCAGAGAATGGGAAGCTGTGGAAAAGACATTCCTTTGGAGACACAGTTCATGCTACTGGAAAGCAAGGATGAGGTTGAAGGGAATGGGGATGATGCTCCTACTGTTTATACCGTCTTTCTCCCTCTCCTTGAAGGCCAGTTCAGAGCTGTTCTGCAAGGAAATGAGAAGAATGAGATTGAGATTTGTTTTGAGAGTGGAGATAAGGCGGTGGAAACTAGCCAGGGAACTCACCTTGTTTATGTTCATGCTGGAACCAATCCCTTTGAAGTTATCAGACAATCCGTAAAGGCTGTGGAGAGACACATGCAGACATTTCATCATCGTGAGAAGAAGAAGCTTCCTTCTTTCCTAGACTGGTTTGGCTGGTGCACATGGGACGCTTTCTACACAGATGTGACTGCCGAGGGTGTTGATGAAGGCCTTAAAAGTCTTTCTGAAGGAGGTACCCCTCCGAAGTTTCTGATCATAGACGATGGTTGGCAACAAATAGAAAACAAAGAGAAAGATGAAAACTGCGTTGTTCAGGAAGGAGCACAGTTTGCTACTAGGCTTGTTGGTATCAAGGAGAATGCAAAATTTCAGAAGAGTGATCAGAAGGATACGCAAGTGTCAGGACTAAAGAGTGTAGTTGACAATGCGAAGCAGCGCCATAATGTGAAGCAAGTTTATGCATGGCATGCTTTAGCTGGATACTGGGGTGGAGTAAAACCCGCTGCTTCTGGAATGGAACACTATGATAGTGCATTAGCATACCCAGTTCAGTCTCCAGGTGTCTTAGGGAACCAACCAGATATAGTAATGGACAGCCTTGCTGTTCATGGTCTCGGACTTGTCAATCCGAAGAAAGTTTTCAACTTCTACAACGAACTGCATTCATACTTGGCTTCATGTGGTATAGATGGAGTCAAAGTTGACGTCCAGAACATCATTGAAACCCTAGGTGCTGGTCTTGGCGGGAGAGTCTCTCTCACTCGTAGCTACCAGCAAGCTTTAGAAGCTTCAATTGCCCGGAACTTCACAGATAATGGTTGCATATCTTGCATGTGTCACAACACAGACGGACTTTACAGCGCAAAACAAACCGCTATTGTTAGAGCTTCCGATGATTTCTACCCAAGAGATCCTGCTTCTCACACCATCCACATTGCATCGGTTGCATACAACAGCCTTTTCCTTGGAGAGTTCATGCAACCTGACTGGGATATGTTCCATAGTCTACACCCAACTGCAGAGTACCATGCTGCAGCGCGTGCAGTGGGTGGATGCGCAATCTATGTCAGTGATAAGCCAGGCAACCACAACTTTGATCTATTGAGGAAGCTGGTTCTTCCTGATGGTTCAGTTCTTCGGGCTAAGCTCCCGGGTAGGCCTACCCGTGACTGCTTATTCGCTGATCCAGCTAGAGATGGAATCAGCTTGCTCAAGATCTGGAACATGAATAAGTTTACTGGTATTGTTGGTGTATTCAATTGTCAAGGTGCTGGTTGGTGCAAGGAAACGAAGAAGAACCAGATCCATGATACTTCTCCTGGCACACTCACGGGTTCAATCCGTGCGGATGATGCTGATCTAATCTCTCAAGTGGCTGGTGAAGATTGGAGTGGAGATTCAATAGTCTATGCATACAGATCAGGTGAGGTGGTAAGACTACCTAAAGGTGCTTCAATTCCTCTCACCCTCAAAGTCCTTGAATATGAACTCTTCCACATCTCTCCTTTGAAGGAAATCACTGAAAACATTTCGTTCGCACCAATAGGACTTGTAGACATGTTCAACTCAAGTGGAGCAATCGAGTCGATTGATATCAATCACGTGACCGACAAGAATCCTGAATTCTTCGATGGAGAGATCTCCTCGGCTTCTCCCGCGCTCAGCGATAACCGTTCTCCAACAGCTCTGGTATCTGTCAGTGTCAGAGGCTGTGGTCGTTTTGGAGCTTACTCTTCTCAGCGTCCTCTAAAATGCGCCGTTGAAAGCACAGAGACTGATTTCACGTACGACGCCGAGGTTGGGCTTGTGACTTTGAATCTGCCGGTGACGAGGGAGGAAATGTTCAGATGGCATGTTGAGATTCTGGTCTAG

Protein Analysis

773

Amino Acids

85.14

Weight (kDa)

5.45

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 7 - 756 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1158
Acc16I TGCGCA 1 cut(s) 1507
Acc65I GGTACC 1 cut(s) 683
AccB1I GGYRCC 1 cut(s) 683
AccI GTMKAC 2 cut(s) 1456, 2004
AccII CGCG 3 cut(s) 140, 1489, 2108
AciI CCGC 6 cut(s) 446, 951, 1194, 1323, 1796, 2106
AclWI GGATC 3 cut(s) 1355, 1628, 1747
AcsI RAATTY 2 cut(s) 815, 2072
AcuI CTGAAG 1 cut(s) 696
AcvI CACGTG 1 cut(s) 2053
AcyI GRCGYC 2 cut(s) 1155, 2241
AfaI GTAC 4 cut(s) 27, 685, 1475, 2237
AfiI CCNNNNNNNGG 3 cut(s) 97, 104, 2250
AflIII ACRYGT 1 cut(s) 2007
AgeI ACCGGT 1 cut(s) 104
AjnI CCWGG 4 cut(s) 459, 1006, 1528, 1768
AleI CACNNNNGTG 1 cut(s) 632
AloI GAACNNNNNNTCC 4 cut(s) 269, 301, 2276, 2308
Alw21I GWGCWC 2 cut(s) 611, 775
Alw26I GTCTC 6 cut(s) 272, 532, 1008, 1067, 1207, 2216
Alw44I GTGCAC 1 cut(s) 607
AlwI GGATC 3 cut(s) 1355, 1628, 1747
AlwNI CAGNNNCTG 2 cut(s) 2160, 2225
Ama87I CYCGRG 1 cut(s) 1600
AoxI GGCC 3 cut(s) 373, 658, 1607
ApaLI GTGCAC 1 cut(s) 607
ApeKI GCWGC 4 cut(s) 883, 953, 1481, 1484
ApoI RAATTY 2 cut(s) 815, 2072
ArsI GACNNNNNNTTYG 6 cut(s) 145, 177, 1140, 1172, 1610, 1642
AsiGI ACCGGT 1 cut(s) 104
Asp700I GAANNNNTTC 1 cut(s) 1088
Asp718I GGTACC 1 cut(s) 683
AspA2I CCTAGG 1 cut(s) 1177
AspLEI GCGC 7 cut(s) 140, 888, 1313, 1489, 1508, 2110, 2207
AsuC2I CCSGG 3 cut(s) 1249, 1601, 1602
AsuHPI GGTGA 5 cut(s) 461, 1841, 1886, 1906, 2287
AvaI CYCGRG 1 cut(s) 1600
AvrII CCTAGG 1 cut(s) 1177
BaeGI GKGCMC 1 cut(s) 611
BaeI ACNNNNGTAYC 4 cut(s) 831, 864, 922, 955
BanI GGYRCC 1 cut(s) 683
BbrPI CACGTG 1 cut(s) 2053
BbsI GAAGAC 3 cut(s) 62, 155, 188
Bbv12I GWGCWC 2 cut(s) 611, 775
BbvI GCAGC 4 cut(s) 895, 940, 1468, 1496
BccI CCATC 8 cut(s) 185, 707, 1133, 1385, 1568, 1640, 2075, 2292
BceAI ACGGC 1 cut(s) 2192
BcgI CGANNNNNNTGC 2 cut(s) 2016, 2050
BciT130I CCWGG 4 cut(s) 461, 1008, 1530, 1770
BciVI GTATCC 2 cut(s) 832, 923
BclI TGATCA 2 cut(s) 702, 829
BcnI CCSGG 3 cut(s) 1249, 1601, 1602
BcoDI GTCTC 6 cut(s) 272, 532, 1008, 1067, 1207, 2216
BfaI CTAG 6 cut(s) 456, 590, 786, 1178, 1641, 2320
BfmI CTRYAG 2 cut(s) 1467, 1482
BfoI RGCGCY 1 cut(s) 889
BfuI GTATCC 2 cut(s) 832, 923
BglII AGATCT 2 cut(s) 1665, 2088
BisI GCNGC 4 cut(s) 884, 954, 1482, 1485
BlnI CCTAGG 1 cut(s) 1177
BlpI GCTNAGC 2 cut(s) 1593, 2111
BlsI GCNGC 4 cut(s) 885, 955, 1483, 1486
Bme1390I CCNGG 7 cut(s) 461, 1008, 1249, 1530, 1601, 1602, 1770
BmeT110I CYCGRG 1 cut(s) 1600
BmiI GGNNCC 3 cut(s) 493, 685, 1022
BmrFI CCNGG 7 cut(s) 461, 1008, 1249, 1530, 1601, 1602, 1770
BmrI ACTGGG 3 cut(s) 943, 989, 1447
BmsI GCATC 4 cut(s) 322, 1397, 1493, 1792
BmuI ACTGGG 3 cut(s) 943, 989, 1447
BpiI GAAGAC 3 cut(s) 62, 155, 188
BplI GAGNNNNNCTC 6 cut(s) 1189, 1221, 1191, 1223, 2078, 2110
BpmI CTGGAG 1 cut(s) 990
Bpu1102I GCTNAGC 2 cut(s) 1593, 2111
BpuEI CTTGAG 3 cut(s) 1646, 1803, 2002
BpuMI CCSGG 3 cut(s) 1249, 1601, 1602
BsaAI YACGTR 2 cut(s) 2053, 2235
BsaBI GATNNNNATC 3 cut(s) 1806, 2042, 2046
BsaHI GRCGYC 2 cut(s) 1155, 2241
BsaI GGTCTC 1 cut(s) 1067
BsaJI CCNNGG 7 cut(s) 460, 642, 1177, 1414, 1600, 2094, 2244
BsaWI WCCGGW 4 cut(s) 97, 104, 122, 216
BsaXI ACNNNNNCTCC 6 cut(s) 269, 299, 1134, 1164, 2276, 2306
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 104, 2250
Bse118I RCCGGY 2 cut(s) 104, 2272
Bse1I ACTGG 7 cut(s) 300, 376, 599, 938, 995, 1442, 1693
Bse3DI GCAATG 1 cut(s) 1383
Bse8I GATNNNNATC 3 cut(s) 1806, 2042, 2046
BseBI CCWGG 4 cut(s) 461, 1008, 1530, 1770
BseDI CCNNGG 7 cut(s) 460, 642, 1177, 1414, 1600, 2094, 2244
BseGI GGATG 6 cut(s) 240, 313, 334, 1377, 1508, 1804
BseJI GATNNNNATC 3 cut(s) 1806, 2042, 2046
BseLI CCNNNNNNNGG 3 cut(s) 97, 104, 2250
BseMI GCAATG 1 cut(s) 1383
BseMII CTCAG 3 cut(s) 254, 2125, 2201
BseNI ACTGG 7 cut(s) 300, 376, 599, 938, 995, 1442, 1693
BseRI GAGGAG 1 cut(s) 2083
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 4 cut(s) 895, 940, 1468, 1496
BsgI GTGCAG 1 cut(s) 1512
Bsh1236I CGCG 3 cut(s) 140, 1489, 2108
BshFI GGCC 3 cut(s) 375, 660, 1609
BshNI GGYRCC 1 cut(s) 683
BshTI ACCGGT 1 cut(s) 104
BsiHKAI GWGCWC 2 cut(s) 611, 775
BsiHKCI CYCGRG 1 cut(s) 1600
BsiSI CCGG 7 cut(s) 98, 105, 123, 217, 1249, 1601, 2273
BsiWI CGTACG 1 cut(s) 2235
BslFI GGGAC 1 cut(s) 629
BslI CCNNNNNNNGG 3 cut(s) 97, 104, 2250
BsmAI GTCTC 6 cut(s) 272, 532, 1008, 1067, 1207, 2216
BsmFI GGGAC 1 cut(s) 629
BsmI GAATGC 2 cut(s) 814, 1111
BsnI GGCC 3 cut(s) 375, 660, 1609
Bso31I GGTCTC 1 cut(s) 1067
BsoBI CYCGRG 1 cut(s) 1600
Bsp1286I GDGCHC 2 cut(s) 611, 775
Bsp1407I TGTACA 1 cut(s) 25
Bsp1720I GCTNAGC 2 cut(s) 1593, 2111
BspACI CCGC 6 cut(s) 446, 951, 1194, 1323, 1796, 2106
BspANI GGCC 3 cut(s) 375, 660, 1609
BspCNI CTCAG 3 cut(s) 253, 2124, 2200
BspFNI CGCG 3 cut(s) 140, 1489, 2108
BspLI GGNNCC 3 cut(s) 493, 685, 1022
BspMAI CTGCAG 2 cut(s) 1471, 1486
BspPI GGATC 3 cut(s) 1355, 1628, 1747
BspT107I GGYRCC 1 cut(s) 683
BspTNI GGTCTC 1 cut(s) 1067
BsrDI GCAATG 1 cut(s) 1383
BsrFI RCCGGY 2 cut(s) 104, 2272
BsrGI TGTACA 1 cut(s) 25
BsrI ACTGG 7 cut(s) 300, 376, 599, 938, 995, 1442, 1693
BssAI RCCGGY 2 cut(s) 104, 2272
BssECI CCNNGG 7 cut(s) 460, 642, 1177, 1414, 1600, 2094, 2244
BssNI GRCGYC 2 cut(s) 1155, 2241
BssT1I CCWWGG 2 cut(s) 1177, 1414
Bst2UI CCWGG 4 cut(s) 461, 1008, 1530, 1770
Bst4CI ACNGT 5 cut(s) 285, 343, 352, 777, 2123
Bst6I CTCTTC 3 cut(s) 818, 1946, 2188
BstACI GRCGYC 2 cut(s) 1155, 2241
BstAPI GCANNNNNTGC 5 cut(s) 779, 917, 1278, 1394, 1490
BstAUI TGTACA 1 cut(s) 25
BstBAI YACGTR 2 cut(s) 2053, 2235
BstC8I GCNNGC 4 cut(s) 918, 1227, 1491, 1658
BstDEI CTNAG 5 cut(s) 240, 1015, 1593, 2111, 2187
BstEII GGTNACC 1 cut(s) 100
BstF5I GGATG 6 cut(s) 240, 313, 334, 1377, 1508, 1804
BstFNI CGCG 3 cut(s) 140, 1489, 2108
BstH2I RGCGCY 1 cut(s) 889
BstHHI GCGC 7 cut(s) 140, 888, 1313, 1489, 1508, 2110, 2207
BstMAI GTCTC 6 cut(s) 272, 532, 1008, 1067, 1207, 2216
BstNI CCWGG 4 cut(s) 461, 1008, 1530, 1770
BstNSI RCATGY 5 cut(s) 547, 920, 1285, 2011, 2306
BstPI GGTNACC 1 cut(s) 100
BstSCI CCNGG 7 cut(s) 459, 1006, 1247, 1528, 1599, 1600, 1768
BstSFI CTRYAG 2 cut(s) 1467, 1482
BstSLI GKGCMC 1 cut(s) 611
BstUI CGCG 3 cut(s) 140, 1489, 2108
BstV1I GCAGC 4 cut(s) 895, 940, 1468, 1496
BstV2I GAAGAC 3 cut(s) 62, 155, 188
BstX2I RGATCY 4 cut(s) 1360, 1665, 1752, 2088
BstYI RGATCY 4 cut(s) 1360, 1665, 1752, 2088
BsuI GTATCC 2 cut(s) 832, 923
BsuRI GGCC 3 cut(s) 375, 660, 1609
BtsCI GGATG 6 cut(s) 240, 313, 334, 1377, 1508, 1804
BtsI GCAGTG 1 cut(s) 1500
BtsIMutI CAGTG 4 cut(s) 1500, 1525, 1968, 2155
Cac8I GCNNGC 4 cut(s) 918, 1227, 1491, 1658
CaiI CAGNNNCTG 2 cut(s) 2160, 2225
CfoI GCGC 7 cut(s) 140, 888, 1313, 1489, 1508, 2110, 2207
Cfr10I RCCGGY 2 cut(s) 104, 2272
Cfr9I CCCGGG 1 cut(s) 1600
CseI GACGC 3 cut(s) 626, 2180, 2249
Csp6I GTAC 4 cut(s) 26, 684, 1474, 2236
CspAI ACCGGT 1 cut(s) 104
CspCI CAANNNNNGTGG 2 cut(s) 2002, 2037
CviQI GTAC 4 cut(s) 26, 684, 1474, 2236
DdeI CTNAG 5 cut(s) 240, 1015, 1593, 2111, 2187
Eam1104I CTCTTC 3 cut(s) 818, 1946, 2188
EarI CTCTTC 3 cut(s) 818, 1946, 2188
Eco130I CCWWGG 2 cut(s) 1177, 1414
Eco147I AGGCCT 2 cut(s) 660, 1609
Eco31I GGTCTC 1 cut(s) 1067
Eco32I GATATC 1 cut(s) 2044
Eco57I CTGAAG 1 cut(s) 696
Eco72I CACGTG 1 cut(s) 2053
Eco88I CYCGRG 1 cut(s) 1600
Eco91I GGTNACC 1 cut(s) 100
EcoO65I GGTNACC 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 2072
EcoRII CCWGG 4 cut(s) 459, 1006, 1528, 1768
EcoRV GATATC 1 cut(s) 2044
EcoT14I CCWWGG 2 cut(s) 1177, 1414
EcoT22I ATGCAT 2 cut(s) 913, 1864
ErhI CCWWGG 2 cut(s) 1177, 1414
FaqI GGGAC 1 cut(s) 629
FauI CCCGC 3 cut(s) 958, 1187, 2113
FbaI TGATCA 2 cut(s) 702, 829
FblI GTMKAC 2 cut(s) 1456, 2004
Fnu4HI GCNGC 4 cut(s) 884, 954, 1482, 1485
FokI GGATG 6 cut(s) 247, 320, 341, 1364, 1515, 1811
Fsp4HI GCNGC 4 cut(s) 884, 954, 1482, 1485
FspBI CTAG 6 cut(s) 456, 590, 786, 1178, 1641, 2320
FspI TGCGCA 1 cut(s) 1507
GlaI GCGC 7 cut(s) 139, 887, 1312, 1488, 1507, 2109, 2206
GluI GCNGC 4 cut(s) 884, 954, 1482, 1485
GsuI CTGGAG 1 cut(s) 990
HaeII RGCGCY 1 cut(s) 889
HaeIII GGCC 3 cut(s) 375, 660, 1609
HapII CCGG 7 cut(s) 98, 105, 123, 217, 1249, 1601, 2273
HgaI GACGC 3 cut(s) 626, 2180, 2249
HhaI GCGC 7 cut(s) 140, 888, 1313, 1489, 1508, 2110, 2207
Hin1I GRCGYC 2 cut(s) 1155, 2241
Hin6I GCGC 7 cut(s) 138, 886, 1311, 1487, 1506, 2108, 2205
HinP1I GCGC 7 cut(s) 138, 886, 1311, 1487, 1506, 2108, 2205
HincII GTYRAC 2 cut(s) 871, 1153
HindII GTYRAC 2 cut(s) 871, 1153
HindIII AAGCTT 3 cut(s) 575, 1227, 1236
HpaII CCGG 7 cut(s) 98, 105, 123, 217, 1249, 1601, 2273
HphI GGTGA 5 cut(s) 461, 1841, 1886, 1906, 2287
Hpy166II GTNNAC 6 cut(s) 609, 871, 1153, 1457, 1686, 2005
Hpy188III TCNNGA 9 cut(s) 563, 764, 852, 960, 1159, 1571, 1663, 1669, 2069
Hpy8I GTNNAC 6 cut(s) 609, 871, 1153, 1457, 1686, 2005
Hpy99I CGWCG 2 cut(s) 145, 2243
HpyAV CCTTC 8 cut(s) 311, 365, 591, 650, 671, 761, 829, 1954
HpyCH4III ACNGT 5 cut(s) 285, 343, 352, 777, 2123
HpyCH4IV ACGT 4 cut(s) 143, 1155, 2052, 2234
HpyF3I CTNAG 5 cut(s) 240, 1015, 1593, 2111, 2187
HpySE526I ACGT 4 cut(s) 143, 1155, 2052, 2234
Hsp92I GRCGYC 2 cut(s) 1155, 2241
HspAI GCGC 7 cut(s) 138, 886, 1311, 1487, 1506, 2108, 2205
KpnI GGTACC 1 cut(s) 687
Ksp22I TGATCA 2 cut(s) 702, 829
LmnI GCTCC 5 cut(s) 340, 770, 1602, 2024, 2174
Lsp1109I GCAGC 4 cut(s) 895, 940, 1468, 1496
LweI GCATC 4 cut(s) 322, 1397, 1493, 1792
MaeI CTAG 6 cut(s) 456, 590, 786, 1178, 1641, 2320
MaeII ACGT 4 cut(s) 143, 1155, 2052, 2234
MaeIII GTNAC 7 cut(s) 100, 634, 1286, 1616, 2053, 2257, 2275
MfeI CAATTG 2 cut(s) 1242, 1708
MflI RGATCY 4 cut(s) 1360, 1665, 1752, 2088
MhlI GDGCHC 2 cut(s) 611, 775
MluCI AATT 5 cut(s) 815, 1242, 1708, 1905, 2072
MlyI GAGTC 5 cut(s) 169, 232, 1152, 1209, 2042
MmeI TCCRAC 1 cut(s) 2153
Mph1103I ATGCAT 2 cut(s) 913, 1864
MroXI GAANNNNTTC 1 cut(s) 1088
MseI TTAA 2 cut(s) 222, 663
MslI CAYNNNNRTG 2 cut(s) 561, 632
MspA1I CMGCKG 1 cut(s) 953
MspI CCGG 7 cut(s) 98, 105, 123, 217, 1249, 1601, 2273
MspR9I CCNGG 7 cut(s) 461, 1008, 1249, 1530, 1601, 1602, 1770
MunI CAATTG 2 cut(s) 1242, 1708
Mva1269I GAATGC 2 cut(s) 814, 1111
MvaI CCWGG 4 cut(s) 461, 1008, 1530, 1770
MvnI CGCG 3 cut(s) 140, 1489, 2108
NciI CCSGG 3 cut(s) 1249, 1601, 1602
NlaIV GGNNCC 3 cut(s) 493, 685, 1022
NmeAIII GCCGAG 3 cut(s) 667, 2075, 2269
NmuCI GTSAC 6 cut(s) 634, 1286, 1616, 2053, 2257, 2275
NsbI TGCGCA 1 cut(s) 1507
NsiI ATGCAT 2 cut(s) 913, 1864
NspI RCATGY 5 cut(s) 547, 920, 1285, 2011, 2306
OliI CACNNNNGTG 1 cut(s) 632
PaeI GCATGC 1 cut(s) 920
PceI AGGCCT 2 cut(s) 660, 1609
PciI ACATGT 1 cut(s) 2007
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PctI GAATGC 2 cut(s) 814, 1111
PdmI GAANNNNTTC 1 cut(s) 1088
PfeI GAWTC 6 cut(s) 114, 1650, 1847, 2065, 2266, 2311
Pfl23II CGTACG 1 cut(s) 2235
PinAI ACCGGT 1 cut(s) 104
PkrI GCNGC 4 cut(s) 885, 955, 1483, 1486
PleI GAGTC 5 cut(s) 168, 232, 1151, 1208, 2041
PmaCI CACGTG 1 cut(s) 2053
PmlI CACGTG 1 cut(s) 2053
PpsI GAGTC 5 cut(s) 168, 232, 1151, 1208, 2041
Ppu21I YACGTR 2 cut(s) 2053, 2235
PscI ACATGT 1 cut(s) 2007
Psp6I CCWGG 4 cut(s) 459, 1006, 1528, 1768
PspCI CACGTG 1 cut(s) 2053
PspEI GGTNACC 1 cut(s) 100
PspGI CCWGG 4 cut(s) 459, 1006, 1528, 1768
PspLI CGTACG 1 cut(s) 2235
PspN4I GGNNCC 3 cut(s) 493, 685, 1022
PstI CTGCAG 2 cut(s) 1471, 1486
PstNI CAGNNNCTG 2 cut(s) 2160, 2225
PsuI RGATCY 4 cut(s) 1360, 1665, 1752, 2088
RsaI GTAC 4 cut(s) 27, 685, 1475, 2237
RsaNI GTAC 4 cut(s) 26, 684, 1474, 2236
RseI CAYNNNNRTG 2 cut(s) 561, 632
SaqAI TTAA 2 cut(s) 222, 663
SatI GCNGC 4 cut(s) 884, 954, 1482, 1485
SchI GAGTC 5 cut(s) 169, 232, 1152, 1209, 2042
ScrFI CCNGG 7 cut(s) 461, 1008, 1249, 1530, 1601, 1602, 1770
SduI GDGCHC 2 cut(s) 611, 775
SfaNI GCATC 4 cut(s) 322, 1397, 1493, 1792
SfcI CTRYAG 2 cut(s) 1467, 1482
SmaI CCCGGG 1 cut(s) 1602
SmiMI CAYNNNNRTG 2 cut(s) 561, 632
SmlI CTYRAG 3 cut(s) 1661, 1818, 2017
SmoI CTYRAG 3 cut(s) 1661, 1818, 2017
SphI GCATGC 1 cut(s) 920
Sse9I AATT 5 cut(s) 815, 1242, 1708, 1905, 2072
SseBI AGGCCT 2 cut(s) 660, 1609
SsiI CCGC 6 cut(s) 446, 951, 1194, 1323, 1796, 2106
SspMI CTAG 6 cut(s) 456, 590, 786, 1178, 1641, 2320
StuI AGGCCT 2 cut(s) 660, 1609
StyD4I CCNGG 7 cut(s) 459, 1006, 1247, 1528, 1599, 1600, 1768
StyI CCWWGG 2 cut(s) 1177, 1414
TaaI ACNGT 5 cut(s) 285, 343, 352, 777, 2123
TaiI ACGT 4 cut(s) 146, 1158, 2055, 2237
TaqI TCGA 3 cut(s) 2031, 2036, 2079
TaqII GACCGA 1 cut(s) 2072
TasI AATT 5 cut(s) 815, 1242, 1708, 1905, 2072
TatI WGTACW 1 cut(s) 25
TfiI GAWTC 6 cut(s) 114, 1650, 1847, 2065, 2266, 2311
Tru1I TTAA 2 cut(s) 222, 663
Tru9I TTAA 2 cut(s) 222, 663
TscAI CASTG 4 cut(s) 1500, 1525, 1975, 2155
TseFI GTSAC 6 cut(s) 634, 1286, 1616, 2053, 2257, 2275
TseI GCWGC 4 cut(s) 883, 953, 1481, 1484
Tsp45I GTSAC 6 cut(s) 634, 1286, 1616, 2053, 2257, 2275
TspGWI ACGGA 3 cut(s) 511, 1314, 1781
TspMI CCCGGG 1 cut(s) 1600
TspRI CASTG 4 cut(s) 1500, 1525, 1975, 2155
VneI GTGCAC 1 cut(s) 607
XapI RAATTY 2 cut(s) 815, 2072
XceI RCATGY 5 cut(s) 547, 920, 1285, 2011, 2306
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XmaI CCCGGG 1 cut(s) 1600
XmaJI CCTAGG 1 cut(s) 1177
XmiI GTMKAC 2 cut(s) 1456, 2004
XmnI GAANNNNTTC 1 cut(s) 1088
XspI CTAG 6 cut(s) 456, 590, 786, 1178, 1641, 2320
ZraI GACGTC 1 cut(s) 1156
Zsp2I ATGCAT 2 cut(s) 913, 1864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.