Rroxscaffold_1G00075890

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000881
Physical Location & Seq
Forward (+)
96461751 .. 96467098
5348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00075890.1

Sequence Viewer

Length: 1851 bp
ATGCTTCACAGCACCGTCGTTTCCCCCAAGGCCCTTCAACTCAATCTCGGCTTCTCTTCATTTCTAGCCACAAACCAGAACCAGAGCAGGAGGAGGAGGAGGAGGAGGATCATCTTTTGCTCACAAACCAGAGATGGTTCCGTGCGTGGTGGAGTTAAGACTAGCACTACTAGTAGTAATGGATGGAGACAATCTATGTTTGTCGGTACGAAACCTGCCCTTGAAGATTCCATTCTGAGTGTCAGTGGTAAACATGTGTTGACAGACGTGCCTCCCAATGTGGTCTACACACCAATTCCCAACTCATCAGCAGCATTCCTTGGCGCCACTTCTCAAAATGCTACTTCACAATCACGCCATGTTTTTAAGCTCGGAGTCTTACGTGATGTCAGATTATTAAGTCTTTTTAGATTTAAACTTTGGTGGATGATACCACGAGTGGGCAGTACAGGAAGTGACATCCCTGTGGAAACTCAGCTGTTGCTCCTAGAAGCAAAGCAGGAGGAGGGAAAGGAAGATAGAACATCATATATCTTATTCTTGCCAGTTCTGGATGGTGAATTTAGAAGCAGCTTGCAGGGAAATACATCCAATGAACTTGAGTTTTGCGTCGAAAGTGGTGACCCCGCTATAGTTGCCTCAGAATCCCTAAAAGCAGTTTTTGTGAATTGTGGGAACCATCCTTTCGATCTGGTGAACGAATCTATGAAGACTGTGGCGAAGCATTATGGAAGCTTTGCTCTTAGAGAAACGAAACAGATGCCTGGAATGCTGGATTGTTTTGGCTGGTGTACTTGGGATGCCTTTTATCAAGGAGTTAACCCTAAAGGAATTAGAGACGGCCTCAGAAGTTTATCAGAAGGAGGTACTCCAGCTAAGTTTTTGATAATCGATGATGGGTGGCAAGATACATCAAATGAGTTCCAAAAAGAAGGGGAGCCATTTGTTGAAGGGACACAGTTTGGAGGCAGATTAAATAGCATTGAAGAAAATAACAAGTTTCGCAGCACAACCAATATGGTCGATAGTGACAAACCAAGTGGTCTCAAGGATTTTGTTTCTGAGATCAAGAATTCCTTTGGCCTTAGGTATGTGTATGTATGGCATGCCCTTTTGGGATATTGGGGAGGGCTTGTTCCAAATGCTCCAGGAACTAAAAAGTATAACCCTAAGTTCAGACACCCAGTACAGTCACCTGGAAATTTGTCAAATATGAGGGATGGAGCTATGGATAGTATGGAGAAATATGGTGTTGGTGTAATAGATCCTGCAAAGGCATATCAATTTTATGATGATCTACACGGATATCTTGTTTCACAGGATGTAGATGGTGTTAAGGTTGATGTTCAGAACATACTGGAGACAGTATCAGCAGGTTTAGGAGGGCGAGTCTCTCTAACTAGACGATTCCAACAGGCACTTGAGAAGTCCATAGCCACTCATTTCCAAGATAACAGCATAATCTGCTGCATGGGCCAAAGCACAGACTCCATTTACCATTCAAAAAGAAGTGCTATTACTCGAGCATCTGATGATTACTACCCAAAAAACCCAACAACACAGACACTGCACATTGCTGCAGTGGCTTTCAACAGCATATTCCTTGGTGAAGTTTTTGTCCCAGATTGGGACATGTTCTATAGCCGGCATGAGGCAGCTGAGTTCCATGCTGCTGCCAGAGCTGTGGGAGGTTGCGGAGTCTATGTAAGTGACAAACCTGGCCAGCATGATTTGAGATACTTAAAAGGCTTGTTCTTCCTGATGGAACAGTTCTCAGAGCTAGATATCCTGGGAGGCCATCACGGGATTGTTTATTTGTTGATCCAGTTATGGACGGGAAGAGTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

616

Amino Acids

68.56

Weight (kDa)

7.26

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 75 - 586 1.2e-188 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 223, 1364
AccB1I GGYRCC 1 cut(s) 323
AccI GTMKAC 1 cut(s) 285
AciI CCGC 2 cut(s) 627, 1695
AclWI GGATC 3 cut(s) 116, 1259, 1816
AcoI YGGCCR 1 cut(s) 1720
AcsI RAATTY 3 cut(s) 560, 1072, 1201
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 5 cut(s) 208, 448, 793, 868, 1188
AfiI CCNNNNNNNGG 3 cut(s) 440, 1627, 1651
AflIII ACRYGT 2 cut(s) 253, 1632
AgsI TTSAA 6 cut(s) 38, 224, 950, 986, 1503, 1591
AhlI ACTAGT 1 cut(s) 170
AjiI CACGTC 1 cut(s) 268
AjnI CCWGG 5 cut(s) 763, 1147, 1195, 1717, 1788
AloI GAACNNNNNNTCC 2 cut(s) 1119, 1151
AluBI AGCT 9 cut(s) 370, 478, 573, 735, 875, 1226, 1658, 1682, 1780
AluI AGCT 9 cut(s) 370, 478, 573, 735, 875, 1226, 1658, 1682, 1780
Alw26I GTCTC 5 cut(s) 181, 831, 1049, 1355, 1396
AlwI GGATC 3 cut(s) 116, 1259, 1816
AlwNI CAGNNNCTG 1 cut(s) 1567
Ama87I CYCGRG 1 cut(s) 1521
AoxI GGCC 6 cut(s) 30, 841, 1081, 1474, 1720, 1795
ApeKI GCWGC 8 cut(s) 311, 570, 1005, 1467, 1577, 1655, 1670, 1673
ApoI RAATTY 3 cut(s) 560, 1072, 1201
AspLEI GCGC 1 cut(s) 326
AspS9I GGNCC 2 cut(s) 31, 1474
AsuHPI GGTGA 5 cut(s) 569, 632, 706, 1185, 1619
AvaI CYCGRG 1 cut(s) 1521
AxyI CCTNAGG 1 cut(s) 1085
BalI TGGCCA 1 cut(s) 1722
BanI GGYRCC 1 cut(s) 323
BarI GAAGNNNNNNTAC 2 cut(s) 1501, 1533
BauI CACGAG 1 cut(s) 435
BbsI GAAGAC 2 cut(s) 716, 1837
BbvI GCAGC 8 cut(s) 323, 582, 1017, 1454, 1564, 1657, 1660, 1667
BccI CCATC 9 cut(s) 128, 177, 548, 687, 890, 1214, 1322, 1756, 1806
BceAI ACGGC 1 cut(s) 856
BcgI CGANNNNNNTGC 2 cut(s) 742, 776
BciT130I CCWGG 5 cut(s) 765, 1149, 1197, 1719, 1790
BcoDI GTCTC 5 cut(s) 181, 831, 1049, 1355, 1396
BcuI ACTAGT 1 cut(s) 170
BfaI CTAG 6 cut(s) 65, 162, 171, 488, 1401, 1781
BfmI CTRYAG 3 cut(s) 630, 1578, 1639
BfoI RGCGCY 1 cut(s) 327
BfuAI ACCTGC 2 cut(s) 223, 1364
BisI GCNGC 8 cut(s) 312, 571, 1006, 1468, 1578, 1656, 1671, 1674
BlsI GCNGC 8 cut(s) 313, 572, 1007, 1469, 1579, 1657, 1672, 1675
Bme1390I CCNGG 5 cut(s) 765, 1149, 1197, 1719, 1790
BmeT110I CYCGRG 1 cut(s) 1521
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 2 cut(s) 31, 1474
BmiI GGNNCC 4 cut(s) 139, 325, 677, 939
BmrFI CCNGG 5 cut(s) 765, 1149, 1197, 1719, 1790
BmrI ACTGGG 1 cut(s) 1178
BmsI GCATC 3 cut(s) 750, 790, 1535
BmuI ACTGGG 1 cut(s) 1178
BpiI GAAGAC 2 cut(s) 716, 1837
BplI GAGNNNNNCTC 2 cut(s) 828, 860
BpmI CTGGAG 3 cut(s) 855, 1131, 1379
BpuEI CTTGAG 3 cut(s) 620, 1031, 1442
Bsa29I ATCGAT 1 cut(s) 891
BsaAI YACGTR 1 cut(s) 383
BsaHI GRCGYC 1 cut(s) 324
BsaI GGTCTC 1 cut(s) 1049
BsaJI CCNNGG 4 cut(s) 27, 319, 1603, 1789
BsaXI ACNNNNNCTCC 6 cut(s) 1119, 1149, 1374, 1404, 1689, 1719
Bsc4I CCNNNNNNNGG 3 cut(s) 440, 1627, 1651
Bse118I RCCGGY 1 cut(s) 1644
Bse1I ACTGG 4 cut(s) 545, 1184, 1362, 1825
Bse21I CCTNAGG 1 cut(s) 1085
Bse3DI GCAATG 1 cut(s) 1572
BseBI CCWGG 5 cut(s) 765, 1149, 1197, 1719, 1790
BseCI ATCGAT 1 cut(s) 891
BseDI CCNNGG 4 cut(s) 27, 319, 1603, 1789
BseGI GGATG 9 cut(s) 188, 432, 459, 559, 587, 679, 805, 1225, 1327
BseLI CCNNNNNNNGG 3 cut(s) 440, 1627, 1651
BseMI GCAATG 1 cut(s) 1572
BseMII CTCAG 7 cut(s) 227, 488, 654, 859, 1053, 1650, 1788
BseNI ACTGG 4 cut(s) 545, 1184, 1362, 1825
BseRI GAGGAG 6 cut(s) 106, 109, 112, 115, 118, 518
BseXI GCAGC 8 cut(s) 323, 582, 1017, 1454, 1564, 1657, 1660, 1667
BsgI GTGCAG 1 cut(s) 1553
BshFI GGCC 6 cut(s) 32, 843, 1083, 1476, 1722, 1797
BshNI GGYRCC 1 cut(s) 323
BshVI ATCGAT 1 cut(s) 891
BsiHKCI CYCGRG 1 cut(s) 1521
BsiSI CCGG 1 cut(s) 1645
BslFI GGGAC 3 cut(s) 967, 1604, 1643
BslI CCNNNNNNNGG 3 cut(s) 440, 1627, 1651
BsmAI GTCTC 5 cut(s) 181, 831, 1049, 1355, 1396
BsmBI CGTCTC 1 cut(s) 831
BsmFI GGGAC 3 cut(s) 967, 1604, 1643
BsmI GAATGC 2 cut(s) 314, 774
BsnI GGCC 6 cut(s) 32, 843, 1083, 1476, 1722, 1797
Bso31I GGTCTC 1 cut(s) 1049
BsoBI CYCGRG 1 cut(s) 1521
Bsp143I GATC 6 cut(s) 108, 688, 1065, 1264, 1294, 1821
BspACI CCGC 2 cut(s) 627, 1695
BspANI GGCC 6 cut(s) 32, 843, 1083, 1476, 1722, 1797
BspCNI CTCAG 7 cut(s) 228, 487, 653, 858, 1054, 1651, 1787
BspDI ATCGAT 1 cut(s) 891
BspLI GGNNCC 4 cut(s) 139, 325, 677, 939
BspMAI CTGCAG 1 cut(s) 1582
BspMI ACCTGC 2 cut(s) 223, 1364
BspPI GGATC 3 cut(s) 116, 1259, 1816
BspT107I GGYRCC 1 cut(s) 323
BspTNI GGTCTC 1 cut(s) 1049
BsrDI GCAATG 1 cut(s) 1572
BsrFI RCCGGY 1 cut(s) 1644
BsrI ACTGG 4 cut(s) 545, 1184, 1362, 1825
BssAI RCCGGY 1 cut(s) 1644
BssECI CCNNGG 4 cut(s) 27, 319, 1603, 1789
BssMI GATC 6 cut(s) 108, 688, 1065, 1264, 1294, 1821
BssNI GRCGYC 1 cut(s) 324
BssSI CACGAG 1 cut(s) 435
BssT1I CCWWGG 3 cut(s) 27, 319, 1603
Bst2BI CACGAG 1 cut(s) 435
Bst2UI CCWGG 5 cut(s) 765, 1149, 1197, 1719, 1790
Bst4CI ACNGT 6 cut(s) 16, 715, 960, 1191, 1366, 1770
Bst6I CTCTTC 2 cut(s) 61, 1834
BstACI GRCGYC 1 cut(s) 324
BstAPI GCANNNNNTGC 1 cut(s) 1464
BstBAI YACGTR 1 cut(s) 383
BstC8I GCNNGC 4 cut(s) 575, 1107, 1646, 1724
BstEII GGTNACC 1 cut(s) 620
BstF5I GGATG 9 cut(s) 188, 432, 459, 559, 587, 679, 805, 1225, 1327
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 1 cut(s) 326
BstKTI GATC 6 cut(s) 111, 691, 1068, 1267, 1297, 1824
BstMAI GTCTC 5 cut(s) 181, 831, 1049, 1355, 1396
BstMBI GATC 6 cut(s) 108, 688, 1065, 1264, 1294, 1821
BstMWI GCNNNNNNNGC 6 cut(s) 635, 769, 1464, 1473, 1583, 1679
BstNI CCWGG 5 cut(s) 765, 1149, 1197, 1719, 1790
BstNSI RCATGY 3 cut(s) 257, 1109, 1636
BstPI GGTNACC 1 cut(s) 620
BstSCI CCNGG 5 cut(s) 763, 1147, 1195, 1717, 1788
BstSFI CTRYAG 3 cut(s) 630, 1578, 1639
BstV1I GCAGC 8 cut(s) 323, 582, 1017, 1454, 1564, 1657, 1660, 1667
BstV2I GAAGAC 2 cut(s) 716, 1837
BstX2I RGATCY 1 cut(s) 1264
BstXI CCANNNNNNTGG 1 cut(s) 1684
BstYI RGATCY 1 cut(s) 1264
Bsu15I ATCGAT 1 cut(s) 891
Bsu36I CCTNAGG 1 cut(s) 1085
BsuRI GGCC 6 cut(s) 32, 843, 1083, 1476, 1722, 1797
BsuTUI ATCGAT 1 cut(s) 891
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 9 cut(s) 188, 432, 459, 559, 587, 679, 805, 1225, 1327
BtsI GCAGTG 2 cut(s) 1565, 1587
BtsIMutI CAGTG 3 cut(s) 250, 1565, 1587
BveI ACCTGC 2 cut(s) 223, 1364
Cac8I GCNNGC 4 cut(s) 575, 1107, 1646, 1724
CaiI CAGNNNCTG 1 cut(s) 1567
CfoI GCGC 1 cut(s) 326
Cfr10I RCCGGY 1 cut(s) 1644
Cfr13I GGNCC 2 cut(s) 31, 1474
ClaI ATCGAT 1 cut(s) 891
CseI GACGC 1 cut(s) 598
Csp6I GTAC 5 cut(s) 207, 447, 792, 867, 1187
CspCI CAANNNNNGTGG 2 cut(s) 1021, 1056
CviAII CATG 8 cut(s) 254, 359, 1106, 1471, 1633, 1649, 1667, 1727
CviQI GTAC 5 cut(s) 207, 447, 792, 867, 1187
DinI GGCGCC 1 cut(s) 325
DpnI GATC 6 cut(s) 110, 690, 1067, 1266, 1296, 1823
DpnII GATC 6 cut(s) 108, 688, 1065, 1264, 1294, 1821
DraI TTTAAA 1 cut(s) 415
EaeI YGGCCR 1 cut(s) 1720
Eam1104I CTCTTC 2 cut(s) 61, 1834
EarI CTCTTC 2 cut(s) 61, 1834
Eco130I CCWWGG 3 cut(s) 27, 319, 1603
Eco31I GGTCTC 1 cut(s) 1049
Eco32I GATATC 2 cut(s) 1307, 1786
Eco81I CCTNAGG 1 cut(s) 1085
Eco88I CYCGRG 1 cut(s) 1521
Eco91I GGTNACC 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 31
EcoO65I GGTNACC 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 1072
EcoRII CCWGG 5 cut(s) 763, 1147, 1195, 1717, 1788
EcoRV GATATC 2 cut(s) 1307, 1786
EcoT14I CCWWGG 3 cut(s) 27, 319, 1603
EgeI GGCGCC 1 cut(s) 325
EheI GGCGCC 1 cut(s) 325
ErhI CCWWGG 3 cut(s) 27, 319, 1603
Esp3I CGTCTC 1 cut(s) 831
FaeI CATG 8 cut(s) 257, 362, 1109, 1474, 1636, 1652, 1670, 1730
FalI AAGNNNNNCTT 2 cut(s) 1061, 1093
FaqI GGGAC 3 cut(s) 967, 1604, 1643
FatI CATG 8 cut(s) 253, 358, 1105, 1470, 1632, 1648, 1666, 1726
FauI CCCGC 1 cut(s) 634
FblI GTMKAC 1 cut(s) 285
Fnu4HI GCNGC 8 cut(s) 312, 571, 1006, 1468, 1578, 1656, 1671, 1674
FokI GGATG 9 cut(s) 195, 439, 446, 566, 574, 666, 812, 1232, 1334
Fsp4HI GCNGC 8 cut(s) 312, 571, 1006, 1468, 1578, 1656, 1671, 1674
FspBI CTAG 6 cut(s) 65, 162, 171, 488, 1401, 1781
GlaI GCGC 1 cut(s) 325
GluI GCNGC 8 cut(s) 312, 571, 1006, 1468, 1578, 1656, 1671, 1674
GsuI CTGGAG 3 cut(s) 855, 1131, 1379
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 6 cut(s) 32, 843, 1083, 1476, 1722, 1797
HapII CCGG 1 cut(s) 1645
HgaI GACGC 1 cut(s) 598
HhaI GCGC 1 cut(s) 326
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 8 cut(s) 257, 362, 1109, 1474, 1636, 1652, 1670, 1730
Hin6I GCGC 1 cut(s) 324
HinP1I GCGC 1 cut(s) 324
HincII GTYRAC 2 cut(s) 261, 820
HindII GTYRAC 2 cut(s) 261, 820
HindIII AAGCTT 1 cut(s) 733
HinfI GANTC 9 cut(s) 227, 375, 644, 701, 1389, 1407, 1487, 1698, 1842
HpaI GTTAAC 1 cut(s) 820
HpaII CCGG 1 cut(s) 1645
HphI GGTGA 5 cut(s) 569, 632, 706, 1185, 1619
Hpy166II GTNNAC 6 cut(s) 251, 261, 286, 697, 792, 820
Hpy188III TCNNGA 3 cut(s) 551, 1069, 1759
Hpy8I GTNNAC 6 cut(s) 251, 261, 286, 697, 792, 820
Hpy99I CGWCG 2 cut(s) 20, 614
HpyAV CCTTC 4 cut(s) 44, 854, 926, 944
HpyCH4III ACNGT 6 cut(s) 16, 715, 960, 1191, 1366, 1770
HpyCH4IV ACGT 2 cut(s) 267, 382
HpyCH4V TGCA 5 cut(s) 577, 1271, 1470, 1570, 1580
HpyF10VI GCNNNNNNNGC 6 cut(s) 635, 769, 1464, 1473, 1583, 1679
HpySE526I ACGT 2 cut(s) 267, 382
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 8 cut(s) 257, 362, 1109, 1474, 1636, 1652, 1670, 1730
HspAI GCGC 1 cut(s) 324
KasI GGCGCC 1 cut(s) 323
KroI GCCGGC 1 cut(s) 1644
KroNI GCCGGC 1 cut(s) 1646
KspAI GTTAAC 1 cut(s) 820
Kzo9I GATC 6 cut(s) 108, 688, 1065, 1264, 1294, 1821
LmnI GCTCC 4 cut(s) 489, 937, 1150, 1223
Lsp1109I GCAGC 8 cut(s) 323, 582, 1017, 1454, 1564, 1657, 1660, 1667
LweI GCATC 3 cut(s) 750, 790, 1535
MaeI CTAG 6 cut(s) 65, 162, 171, 488, 1401, 1781
MaeII ACGT 2 cut(s) 267, 382
MaeIII GTNAC 5 cut(s) 455, 620, 1028, 1191, 1709
MalI GATC 6 cut(s) 110, 690, 1067, 1266, 1296, 1823
MboI GATC 6 cut(s) 108, 688, 1065, 1264, 1294, 1821
MboII GAAGA 8 cut(s) 48, 236, 527, 721, 998, 1747, 1837, 1851
MflI RGATCY 1 cut(s) 1264
MlsI TGGCCA 1 cut(s) 1722
MluCI AATT 7 cut(s) 294, 560, 667, 831, 1072, 1201, 1283
MluNI TGGCCA 1 cut(s) 1722
Mly113I GGCGCC 1 cut(s) 324
MlyI GAGTC 5 cut(s) 384, 1398, 1481, 1707, 1851
MmeI TCCRAC 1 cut(s) 1435
Mox20I TGGCCA 1 cut(s) 1722
MroNI GCCGGC 1 cut(s) 1644
MscI TGGCCA 1 cut(s) 1722
MseI TTAA 8 cut(s) 156, 366, 398, 414, 819, 974, 1335, 1742
MslI CAYNNNNRTG 1 cut(s) 464
Msp20I TGGCCA 1 cut(s) 1722
MspA1I CMGCKG 2 cut(s) 478, 1658
MspI CCGG 1 cut(s) 1645
MspR9I CCNGG 5 cut(s) 765, 1149, 1197, 1719, 1790
Mva1269I GAATGC 2 cut(s) 314, 774
MvaI CCWGG 5 cut(s) 765, 1149, 1197, 1719, 1790
MwoI GCNNNNNNNGC 6 cut(s) 635, 769, 1464, 1473, 1583, 1679
NaeI GCCGGC 1 cut(s) 1646
NarI GGCGCC 1 cut(s) 324
NdeII GATC 6 cut(s) 108, 688, 1065, 1264, 1294, 1821
NgoMIV GCCGGC 1 cut(s) 1644
NlaIII CATG 8 cut(s) 257, 362, 1109, 1474, 1636, 1652, 1670, 1730
NlaIV GGNNCC 4 cut(s) 139, 325, 677, 939
NmeAIII GCCGAG 1 cut(s) 27
NmuCI GTSAC 5 cut(s) 455, 620, 1028, 1191, 1709
NspI RCATGY 3 cut(s) 257, 1109, 1636
PaeI GCATGC 1 cut(s) 1109
PaeR7I CTCGAG 1 cut(s) 1521
PciI ACATGT 2 cut(s) 253, 1632
PctI GAATGC 2 cut(s) 314, 774
PdiI GCCGGC 1 cut(s) 1646
PfeI GAWTC 4 cut(s) 227, 644, 701, 1407
PfoI TCCNGGA 1 cut(s) 1147
PkrI GCNGC 8 cut(s) 313, 572, 1007, 1469, 1579, 1657, 1672, 1675
PleI GAGTC 5 cut(s) 383, 1397, 1481, 1706, 1850
PluTI GGCGCC 1 cut(s) 327
PpsI GAGTC 5 cut(s) 383, 1397, 1481, 1706, 1850
Ppu21I YACGTR 1 cut(s) 383
PscI ACATGT 2 cut(s) 253, 1632
Psp6I CCWGG 5 cut(s) 763, 1147, 1195, 1717, 1788
PspEI GGTNACC 1 cut(s) 620
PspGI CCWGG 5 cut(s) 763, 1147, 1195, 1717, 1788
PspN4I GGNNCC 4 cut(s) 139, 325, 677, 939
PspPI GGNCC 2 cut(s) 31, 1474
PspXI VCTCGAGB 1 cut(s) 1521
PstI CTGCAG 1 cut(s) 1582
PstNI CAGNNNCTG 1 cut(s) 1567
PsuI RGATCY 1 cut(s) 1264
PvuII CAGCTG 2 cut(s) 478, 1658
RsaI GTAC 5 cut(s) 208, 448, 793, 868, 1188
RsaNI GTAC 5 cut(s) 207, 447, 792, 867, 1187
RseI CAYNNNNRTG 1 cut(s) 464
SaqAI TTAA 8 cut(s) 156, 366, 398, 414, 819, 974, 1335, 1742
SatI GCNGC 8 cut(s) 312, 571, 1006, 1468, 1578, 1656, 1671, 1674
Sau3AI GATC 6 cut(s) 108, 688, 1065, 1264, 1294, 1821
Sau96I GGNCC 2 cut(s) 31, 1474
SchI GAGTC 5 cut(s) 384, 1398, 1481, 1707, 1851
ScrFI CCNGG 5 cut(s) 765, 1149, 1197, 1719, 1790
SfaNI GCATC 3 cut(s) 750, 790, 1535
SfcI CTRYAG 3 cut(s) 630, 1578, 1639
SfoI GGCGCC 1 cut(s) 325
Sfr274I CTCGAG 1 cut(s) 1521
SlaI CTCGAG 1 cut(s) 1521
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 4 cut(s) 599, 1046, 1421, 1521
SmoI CTYRAG 4 cut(s) 599, 1046, 1421, 1521
SpeI ACTAGT 1 cut(s) 170
SphI GCATGC 1 cut(s) 1109
Sse9I AATT 7 cut(s) 294, 560, 667, 831, 1072, 1201, 1283
SsiI CCGC 2 cut(s) 627, 1695
SspDI GGCGCC 1 cut(s) 323
SspMI CTAG 6 cut(s) 65, 162, 171, 488, 1401, 1781
StyD4I CCNGG 5 cut(s) 763, 1147, 1195, 1717, 1788
StyI CCWWGG 3 cut(s) 27, 319, 1603
TaaI ACNGT 6 cut(s) 16, 715, 960, 1191, 1366, 1770
TaiI ACGT 2 cut(s) 270, 385
TaqI TCGA 5 cut(s) 612, 687, 891, 1023, 1522
TasI AATT 7 cut(s) 294, 560, 667, 831, 1072, 1201, 1283
TatI WGTACW 3 cut(s) 446, 791, 1186
TfiI GAWTC 4 cut(s) 227, 644, 701, 1407
Tru1I TTAA 8 cut(s) 156, 366, 398, 414, 819, 974, 1335, 1742
Tru9I TTAA 8 cut(s) 156, 366, 398, 414, 819, 974, 1335, 1742
TscAI CASTG 3 cut(s) 250, 1572, 1587
TseFI GTSAC 5 cut(s) 455, 620, 1028, 1191, 1709
TseI GCWGC 8 cut(s) 311, 570, 1005, 1467, 1577, 1655, 1670, 1673
Tsp45I GTSAC 5 cut(s) 455, 620, 1028, 1191, 1709
TspDTI ATGAA 3 cut(s) 48, 609, 722
TspGWI ACGGA 2 cut(s) 130, 1317
TspRI CASTG 3 cut(s) 250, 1572, 1587
XapI RAATTY 3 cut(s) 560, 1072, 1201
XceI RCATGY 3 cut(s) 257, 1109, 1636
XhoI CTCGAG 1 cut(s) 1521
XmiI GTMKAC 1 cut(s) 285
XspI CTAG 6 cut(s) 65, 162, 171, 488, 1401, 1781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.