Rh7DG125800

Galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000881
Physical Location & Seq
Reverse (-)
10627396 .. 10630589
3194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG125800.1

Sequence Viewer

Length: 1548 bp
ATGTCTATGACCTCTACCGAGCCAAGCAGCAGAGATGGTTGCTTCATGGTCCGTGACAATGCCCTTCTGACACGTGTCCCGGCCAACATCACCGTCACTCCGGCCACTGATAAATCAGCTTTCATCGGAGCCACCTCTGAAGACCGTAGCTCTCGCCATGTTTTCCCTCTCGGACTTCTTGAGGGATACAAGTTTATGTGCCTGTTCAGATTCAAAATCTGGTGGATGATACCGAGTTTTGGGGATTCTGGATGTGATGTTCCGGCAGAGACTCAAATGCTTCTCTTGGAAGCAACGCAAAAGTCGAGTAGTAGTAGTATTCCCGACAAAAATGGGTTCTCATCTGAGTCTGAGGAGCCAACAAGTGCTGAGAACAAAAGCACTTTCTACATTCTGCTATTGCCAGTCTTGGATGGAGCATTCAGGGCTACTTTGCAAGGCAACACTGTTAATGAGCTTGAGTTCAAGGGCTACCCCTCGGGAAAGTCATCATTTCCTAATCTCCGCGAGTTTGTGAAAACCATCAAAGAGGAACATGGACTGAAGTTAGTATATGCATGGCACGCTTTACTTGGTTCATGGGGAGGGCTACTTCCAACATCTGAAGCACTGAGGAAATACAATCCCCAAATCAAATCCATAGTTCAGTCTCCTGGTAACAAGAGTAACGTCATATGTGGAACCCTCGATCCAATGGAGAAACATGGGATCGGAATGATTGACCCTTCGATGATTTATAGATTTTACGATGATCTACATAGCTACCTTGCAAGTTGCAATATAGACGGAGTGAAGGTGGATATACACAATGAAGTGGAACTCCTTGCTTCTGGTTATGGAGGCCGCGTTGCATTGATGAGGCATTTCCAAGAAGCCCTCAAGGAATCTGTCATGAGAAACTTTGGCAGCAATAACCTTCTTTGTTCCATGAGTCTAAGCAATGACTACATTTACAGCTCAAAGAAGAGTGCAGCTTCAAGAGTGCCAGAGGACTTCATGCCACTAGAGAAAACATTTCAGACCCTACATGTTGCTGCAGTAGCTTTTAACAGTCTTCTTATGGGAGAGATTGTTGTACCAGATTGGGACATGTTCTTTAGTGACCACTACACCCGGGAGTTCCATGCTGCAGCCAGAGCATTGGGTGGATGTCCAGTATATGTAAGTGACAAGCCTGGCAGCCATAATTTCAATGTCCTCAAAAAGCTTGTACTGCCTGATGGCTCTATTCTAAGAGCTAGATTCGCTGGCCGGCCTACTAGGGACTGCTTATTCAGTGACCCTGTGGTCGATGGAAAAAGTCTGCTGAAGATTTGGAATCTAAACAAGGTATCAGGAGTTATTGGTGTTTTCAATTGCCAAAGAGCAGGAAAGTGGCCACCAATTGCAGGTGCTCAATATGTGCCTTCCTCTGAATCTGCACCGCCTCTTATAGGCCTCGTCAGCCCCATAGATATTAATATGCTTGAAGATGTTGCAAATGAAAGTTGGAGAGGAGAATGTGCAGTATATGCATACCATTCAGGTCACCCTATACCATCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

515

Amino Acids

56.81

Weight (kDa)

6.25

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 12 - 154 3e-29 Raffinose synthase or seed imbibition protein Sip1
Raffinose_syn PF05691 167 - 509 1.5e-120 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1379
AasI GACNNNNNNGTC 1 cut(s) 1286
Acc36I ACCTGC 1 cut(s) 1379
AccII CGCG 2 cut(s) 507, 846
AciI CCGC 3 cut(s) 505, 844, 1424
AclWI GGATC 2 cut(s) 683, 716
AcoI YGGCCR 4 cut(s) 81, 102, 1249, 1376
AcuI CTGAAG 4 cut(s) 159, 563, 624, 1328
AcvI CACGTG 1 cut(s) 74
AfaI GTAC 2 cut(s) 1077, 1212
AfiI CCNNNNNNNGG 3 cut(s) 239, 1388, 1433
AflIII ACRYGT 4 cut(s) 71, 73, 1027, 1089
AgsI TTSAA 6 cut(s) 214, 466, 978, 1192, 1354, 1469
AjnI CCWGG 2 cut(s) 652, 1174
AloI GAACNNNNNNTCC 4 cut(s) 243, 275, 673, 705
AluBI AGCT 9 cut(s) 119, 150, 457, 762, 957, 974, 1043, 1207, 1238
AluI AGCT 9 cut(s) 119, 150, 457, 762, 957, 974, 1043, 1207, 1238
Alw21I GWGCWC 1 cut(s) 1396
Alw26I GTCTC 2 cut(s) 263, 654
AlwI GGATC 2 cut(s) 683, 716
Ama87I CYCGRG 2 cut(s) 478, 1113
AoxI GGCC 7 cut(s) 81, 102, 841, 1249, 1253, 1376, 1435
ApeKI GCWGC 7 cut(s) 27, 906, 971, 1034, 1127, 1130, 1179
AseI ATTAAT 1 cut(s) 1458
AspS9I GGNCC 1 cut(s) 49
AsuC2I CCSGG 3 cut(s) 80, 1114, 1115
AsuHPI GGTGA 2 cut(s) 82, 1520
AvaI CYCGRG 2 cut(s) 478, 1113
AvaII GGWCC 1 cut(s) 49
BalI TGGCCA 1 cut(s) 1378
BbrPI CACGTG 1 cut(s) 74
BbsI GAAGAC 2 cut(s) 147, 1046
Bbv12I GWGCWC 1 cut(s) 1396
BbvI GCAGC 7 cut(s) 39, 918, 983, 1021, 1114, 1142, 1191
BccI CCATC 6 cut(s) 29, 407, 530, 1214, 1286, 1546
BciT130I CCWGG 2 cut(s) 654, 1176
BciVI GTATCC 1 cut(s) 179
BcnI CCSGG 3 cut(s) 80, 1114, 1115
BcoDI GTCTC 2 cut(s) 263, 654
BfaI CTAG 3 cut(s) 1004, 1239, 1260
BfmI CTRYAG 2 cut(s) 1035, 1128
BfuAI ACCTGC 1 cut(s) 1379
BfuI GTATCC 1 cut(s) 179
BisI GCNGC 8 cut(s) 28, 844, 907, 972, 1035, 1128, 1131, 1180
BlsI GCNGC 8 cut(s) 29, 845, 908, 973, 1036, 1129, 1132, 1181
Bme1390I CCNGG 5 cut(s) 80, 654, 1114, 1115, 1176
Bme18I GGWCC 1 cut(s) 49
BmeT110I CYCGRG 2 cut(s) 478, 1113
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 3 cut(s) 130, 357, 682
BmrFI CCNGG 5 cut(s) 80, 654, 1114, 1115, 1176
BoxI GACNNNNGTC 1 cut(s) 74
BpiI GAAGAC 2 cut(s) 147, 1046
BpuEI CTTGAG 3 cut(s) 200, 479, 863
BpuMI CCSGG 3 cut(s) 80, 1114, 1115
BsaAI YACGTR 1 cut(s) 74
BsaJI CCNNGG 2 cut(s) 477, 1113
BsaXI ACNNNNNCTCC 8 cut(s) 82, 112, 780, 810, 831, 861, 1056, 1086
Bsc4I CCNNNNNNNGG 3 cut(s) 239, 1388, 1433
Bse118I RCCGGY 1 cut(s) 1251
Bse1I ACTGG 2 cut(s) 404, 1154
Bse3DI GCAATG 1 cut(s) 946
BseBI CCWGG 2 cut(s) 654, 1176
BseDI CCNNGG 2 cut(s) 477, 1113
BseGI GGATG 4 cut(s) 231, 257, 418, 1154
BseLI CCNNNNNNNGG 3 cut(s) 239, 1388, 1433
BseMI GCAATG 1 cut(s) 946
BseMII CTCAG 4 cut(s) 336, 342, 360, 602
BseNI ACTGG 2 cut(s) 404, 1154
BseRI GAGGAG 2 cut(s) 368, 1509
BseXI GCAGC 7 cut(s) 39, 918, 983, 1021, 1114, 1142, 1191
BsgI GTGCAG 3 cut(s) 990, 1404, 1524
Bsh1236I CGCG 2 cut(s) 507, 846
BshFI GGCC 7 cut(s) 83, 104, 843, 1251, 1255, 1378, 1437
BsiHKAI GWGCWC 1 cut(s) 1396
BsiHKCI CYCGRG 2 cut(s) 478, 1113
BsiSI CCGG 5 cut(s) 80, 101, 263, 1114, 1252
BslFI GGGAC 3 cut(s) 62, 1100, 1277
BslI CCNNNNNNNGG 3 cut(s) 239, 1388, 1433
BsmAI GTCTC 2 cut(s) 263, 654
BsmFI GGGAC 3 cut(s) 62, 1100, 1277
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 7 cut(s) 83, 104, 843, 1251, 1255, 1378, 1437
BsoBI CYCGRG 2 cut(s) 478, 1113
Bsp1286I GDGCHC 1 cut(s) 1396
Bsp143I GATC 3 cut(s) 688, 708, 751
BspACI CCGC 3 cut(s) 505, 844, 1424
BspANI GGCC 7 cut(s) 83, 104, 843, 1251, 1255, 1378, 1437
BspCNI CTCAG 4 cut(s) 337, 343, 361, 603
BspFNI CGCG 2 cut(s) 507, 846
BspHI TCATGA 1 cut(s) 891
BspLI GGNNCC 3 cut(s) 130, 357, 682
BspMAI CTGCAG 2 cut(s) 1039, 1132
BspMI ACCTGC 1 cut(s) 1379
BspPI GGATC 2 cut(s) 683, 716
BsrDI GCAATG 1 cut(s) 946
BsrFI RCCGGY 1 cut(s) 1251
BsrI ACTGG 2 cut(s) 404, 1154
BssAI RCCGGY 1 cut(s) 1251
BssECI CCNNGG 2 cut(s) 477, 1113
BssMI GATC 3 cut(s) 688, 708, 751
Bst2UI CCWGG 2 cut(s) 654, 1176
Bst4CI ACNGT 4 cut(s) 94, 146, 448, 1052
Bst6I CTCTTC 1 cut(s) 959
BstAPI GCANNNNNTGC 1 cut(s) 1511
BstBAI YACGTR 1 cut(s) 74
BstC8I GCNNGC 3 cut(s) 564, 1249, 1253
BstDEI CTNAG 6 cut(s) 345, 351, 369, 611, 935, 1232
BstEII GGTNACC 1 cut(s) 1526
BstENI CCTNNNNNAGG 1 cut(s) 1431
BstF5I GGATG 4 cut(s) 231, 257, 418, 1154
BstFNI CGCG 2 cut(s) 507, 846
BstKTI GATC 3 cut(s) 691, 711, 754
BstMAI GTCTC 2 cut(s) 263, 654
BstMBI GATC 3 cut(s) 688, 708, 751
BstMWI GCNNNNNNNGC 8 cut(s) 425, 563, 1040, 1136, 1213, 1244, 1443, 1511
BstNI CCWGG 2 cut(s) 654, 1176
BstNSI RCATGY 2 cut(s) 1031, 1093
BstPAI GACNNNNGTC 1 cut(s) 74
BstPI GGTNACC 1 cut(s) 1526
BstSCI CCNGG 5 cut(s) 78, 652, 1112, 1113, 1174
BstSFI CTRYAG 2 cut(s) 1035, 1128
BstUI CGCG 2 cut(s) 507, 846
BstV1I GCAGC 7 cut(s) 39, 918, 983, 1021, 1114, 1142, 1191
BstV2I GAAGAC 2 cut(s) 147, 1046
BstXI CCANNNNNNTGG 1 cut(s) 1141
BsuI GTATCC 1 cut(s) 179
BsuRI GGCC 7 cut(s) 83, 104, 843, 1251, 1255, 1378, 1437
BtsCI GGATG 4 cut(s) 231, 257, 418, 1154
BtsIMutI CAGTG 4 cut(s) 105, 444, 608, 1282
BveI ACCTGC 1 cut(s) 1379
Cac8I GCNNGC 3 cut(s) 564, 1249, 1253
CciI TCATGA 1 cut(s) 891
Cfr10I RCCGGY 1 cut(s) 1251
Cfr13I GGNCC 1 cut(s) 49
Cfr9I CCCGGG 1 cut(s) 1113
Csp6I GTAC 2 cut(s) 1076, 1211
CviQI GTAC 2 cut(s) 1076, 1211
DdeI CTNAG 6 cut(s) 345, 351, 369, 611, 935, 1232
DpnI GATC 3 cut(s) 690, 710, 753
DpnII GATC 3 cut(s) 688, 708, 751
DrdI GACNNNNNNGTC 1 cut(s) 1286
DseDI GACNNNNNNGTC 1 cut(s) 1286
EaeI YGGCCR 4 cut(s) 81, 102, 1249, 1376
Eam1104I CTCTTC 1 cut(s) 959
EarI CTCTTC 1 cut(s) 959
Eco147I AGGCCT 1 cut(s) 1437
Eco47I GGWCC 1 cut(s) 49
Eco57I CTGAAG 4 cut(s) 159, 563, 624, 1328
Eco72I CACGTG 1 cut(s) 74
Eco88I CYCGRG 2 cut(s) 478, 1113
Eco91I GGTNACC 1 cut(s) 1526
EcoNI CCTNNNNNAGG 1 cut(s) 1431
EcoO65I GGTNACC 1 cut(s) 1526
EcoRII CCWGG 2 cut(s) 652, 1174
EcoT22I ATGCAT 2 cut(s) 559, 1516
FaqI GGGAC 3 cut(s) 62, 1100, 1277
FauNDI CATATG 1 cut(s) 674
Fnu4HI GCNGC 8 cut(s) 28, 844, 907, 972, 1035, 1128, 1131, 1180
FokI GGATG 4 cut(s) 238, 264, 425, 1161
FseI GGCCGGCC 1 cut(s) 1255
Fsp4HI GCNGC 8 cut(s) 28, 844, 907, 972, 1035, 1128, 1131, 1180
FspBI CTAG 3 cut(s) 1004, 1239, 1260
GluI GCNGC 8 cut(s) 28, 844, 907, 972, 1035, 1128, 1131, 1180
HaeIII GGCC 7 cut(s) 83, 104, 843, 1251, 1255, 1378, 1437
HapII CCGG 5 cut(s) 80, 101, 263, 1114, 1252
HindIII AAGCTT 1 cut(s) 1205
HinfI GANTC 9 cut(s) 210, 245, 271, 347, 884, 931, 1242, 1318, 1415
HpaII CCGG 5 cut(s) 80, 101, 263, 1114, 1252
HphI GGTGA 2 cut(s) 82, 1520
Hpy188III TCNNGA 7 cut(s) 179, 249, 323, 480, 892, 978, 1335
HpyAV CCTTC 5 cut(s) 74, 735, 787, 926, 1416
HpyCH4III ACNGT 4 cut(s) 94, 146, 448, 1052
HpyCH4IV ACGT 2 cut(s) 73, 669
HpyF10VI GCNNNNNNNGC 8 cut(s) 425, 563, 1040, 1136, 1213, 1244, 1443, 1511
HpyF3I CTNAG 6 cut(s) 345, 351, 369, 611, 935, 1232
HpySE526I ACGT 2 cut(s) 73, 669
KroI GCCGGC 1 cut(s) 1251
KroNI GCCGGC 1 cut(s) 1253
Kzo9I GATC 3 cut(s) 688, 708, 751
LmnI GCTCC 3 cut(s) 128, 355, 416
Lsp1109I GCAGC 7 cut(s) 39, 918, 983, 1021, 1114, 1142, 1191
MaeI CTAG 3 cut(s) 1004, 1239, 1260
MaeII ACGT 2 cut(s) 73, 669
MaeIII GTNAC 8 cut(s) 53, 94, 656, 665, 1100, 1166, 1277, 1526
MalI GATC 3 cut(s) 690, 710, 753
MboI GATC 3 cut(s) 688, 708, 751
MboII GAAGA 5 cut(s) 152, 976, 1046, 1321, 1481
MfeI CAATTG 2 cut(s) 1354, 1383
MhlI GDGCHC 1 cut(s) 1396
MlsI TGGCCA 1 cut(s) 1378
MluCI AATT 3 cut(s) 1186, 1354, 1383
MluNI TGGCCA 1 cut(s) 1378
MlyI GAGTC 3 cut(s) 265, 356, 940
MmeI TCCRAC 2 cut(s) 620, 1469
Mox20I TGGCCA 1 cut(s) 1378
Mph1103I ATGCAT 2 cut(s) 559, 1516
MroNI GCCGGC 1 cut(s) 1251
MscI TGGCCA 1 cut(s) 1378
MseI TTAA 3 cut(s) 450, 1047, 1458
Msp20I TGGCCA 1 cut(s) 1378
MspI CCGG 5 cut(s) 80, 101, 263, 1114, 1252
MspR9I CCNGG 5 cut(s) 80, 654, 1114, 1115, 1176
MunI CAATTG 2 cut(s) 1354, 1383
Mva1269I GAATGC 1 cut(s) 419
MvaI CCWGG 2 cut(s) 654, 1176
MvnI CGCG 2 cut(s) 507, 846
MwoI GCNNNNNNNGC 8 cut(s) 425, 563, 1040, 1136, 1213, 1244, 1443, 1511
NaeI GCCGGC 1 cut(s) 1253
NciI CCSGG 3 cut(s) 80, 1114, 1115
NdeI CATATG 1 cut(s) 674
NdeII GATC 3 cut(s) 688, 708, 751
NgoMIV GCCGGC 1 cut(s) 1251
NlaIV GGNNCC 3 cut(s) 130, 357, 682
NmuCI GTSAC 6 cut(s) 53, 94, 1100, 1166, 1277, 1526
NsiI ATGCAT 2 cut(s) 559, 1516
NspI RCATGY 2 cut(s) 1031, 1093
PagI TCATGA 1 cut(s) 891
PaqCI CACCTGC 1 cut(s) 1379
PceI AGGCCT 1 cut(s) 1437
PciI ACATGT 2 cut(s) 1027, 1089
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PctI GAATGC 1 cut(s) 419
PdiI GCCGGC 1 cut(s) 1253
PfeI GAWTC 6 cut(s) 210, 245, 884, 1242, 1318, 1415
PkrI GCNGC 8 cut(s) 29, 845, 908, 973, 1036, 1129, 1132, 1181
PleI GAGTC 3 cut(s) 265, 355, 939
PmaCI CACGTG 1 cut(s) 74
PmlI CACGTG 1 cut(s) 74
PpsI GAGTC 3 cut(s) 265, 355, 939
Ppu21I YACGTR 1 cut(s) 74
PscI ACATGT 2 cut(s) 1027, 1089
PshAI GACNNNNGTC 1 cut(s) 74
PshBI ATTAAT 1 cut(s) 1458
Psp6I CCWGG 2 cut(s) 652, 1174
PspCI CACGTG 1 cut(s) 74
PspEI GGTNACC 1 cut(s) 1526
PspGI CCWGG 2 cut(s) 652, 1174
PspN4I GGNNCC 3 cut(s) 130, 357, 682
PspPI GGNCC 1 cut(s) 49
PstI CTGCAG 2 cut(s) 1039, 1132
RigI GGCCGGCC 1 cut(s) 1255
RsaI GTAC 2 cut(s) 1077, 1212
RsaNI GTAC 2 cut(s) 1076, 1211
SaqAI TTAA 3 cut(s) 450, 1047, 1458
SatI GCNGC 8 cut(s) 28, 844, 907, 972, 1035, 1128, 1131, 1180
Sau3AI GATC 3 cut(s) 688, 708, 751
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 3 cut(s) 265, 356, 940
ScrFI CCNGG 5 cut(s) 80, 654, 1114, 1115, 1176
SduI GDGCHC 1 cut(s) 1396
SfcI CTRYAG 2 cut(s) 1035, 1128
SinI GGWCC 1 cut(s) 49
SmaI CCCGGG 1 cut(s) 1115
SmlI CTYRAG 3 cut(s) 179, 458, 878
SmoI CTYRAG 3 cut(s) 179, 458, 878
Sse9I AATT 3 cut(s) 1186, 1354, 1383
SseBI AGGCCT 1 cut(s) 1437
SsiI CCGC 3 cut(s) 505, 844, 1424
SspMI CTAG 3 cut(s) 1004, 1239, 1260
StuI AGGCCT 1 cut(s) 1437
StyD4I CCNGG 5 cut(s) 78, 652, 1112, 1113, 1174
TaaI ACNGT 4 cut(s) 94, 146, 448, 1052
TaiI ACGT 2 cut(s) 76, 672
TaqI TCGA 4 cut(s) 305, 687, 728, 1290
TasI AATT 3 cut(s) 1186, 1354, 1383
TatI WGTACW 1 cut(s) 1210
TauI GCSGC 1 cut(s) 846
TfiI GAWTC 6 cut(s) 210, 245, 884, 1242, 1318, 1415
Tru1I TTAA 3 cut(s) 450, 1047, 1458
Tru9I TTAA 3 cut(s) 450, 1047, 1458
TscAI CASTG 4 cut(s) 112, 451, 615, 1282
TseFI GTSAC 6 cut(s) 53, 94, 1100, 1166, 1277, 1526
TseI GCWGC 7 cut(s) 27, 906, 971, 1034, 1127, 1130, 1179
Tsp45I GTSAC 6 cut(s) 53, 94, 1100, 1166, 1277, 1526
TspDTI ATGAA 6 cut(s) 34, 112, 567, 825, 985, 1497
TspGWI ACGGA 2 cut(s) 41, 801
TspMI CCCGGG 1 cut(s) 1113
TspRI CASTG 4 cut(s) 112, 451, 615, 1282
VpaK11BI GGWCC 1 cut(s) 49
VspI ATTAAT 1 cut(s) 1458
XagI CCTNNNNNAGG 1 cut(s) 1431
XceI RCATGY 2 cut(s) 1031, 1093
XmaI CCCGGG 1 cut(s) 1113
XspI CTAG 3 cut(s) 1004, 1239, 1260
Zsp2I ATGCAT 2 cut(s) 559, 1516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.