RchiOBHm_Chr7g0192021

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000881
Physical Location & Seq
Reverse (-)
10646806 .. 10648221
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17159

Sequence Viewer

Length: 669 bp
ATGTCTATGACCTCTACCGAGCCAAGCAGCAGAGATGGTTGCTTCATGGTCCGTGACAATGCCCTTCTGACACGTGTCCCGGCCAACATCACCGTCACTCCGGCCACTGATAAATCAGCTTTCATCGGAGCCACCTCTGAAGACCGTAGCTCTCGCCATGTTTTCCCTCTCGGACTTCTTGAGGGATACAAGTTTATGTGCCTGTTCAGATTCAAAATCTGGTGGATGATACCGAGTTTTGGGGATTCTGGATGTGATGTTCCGGCAGAGACTCAAATGCTTCTCTTGGAAGCAACGCAAACGTCGAGTAGTAGTAGTATTCCCGACAAGAGGAGCCAACAAGTGCTGAGAACAAAAGCACTTTCTACATTCTGCTATTGCCAGTCTTGGATGGAGCATTCAGGGCTACTTTGCAAGGCAACACTGTTAATGAGCTTGAGTTTTGCGTTGAAAGCGATACCAGCACATGTAGACTGGTTTGGATGGAACACATGGGATGCATTTTATAATGACGTTGATCCGGAAGGAATCGAGCTTGGACTCAAAAGGTTTGCATTAATTCTGTCAATCTTTCCGAAGGAGGGTGCCCCCCGAAATGTGTACATCGATGAAGGATGGCAGACTAAAGTAATTGAAAATGAAACTGAACTACCAAATACAGCGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

222

Amino Acids

24.91

Weight (kDa)

5.44

Isoelectric Point (pI)

52.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 12 - 105 2.3e-23 Raffinose synthase or seed imbibition protein Sip1
Raffinose_syn PF05691 151 - 215 1.7e-13 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 507
AccB1I GGYRCC 1 cut(s) 584
AccI GTMKAC 1 cut(s) 471
AccIII TCCGGA 1 cut(s) 520
AclWI GGATC 1 cut(s) 512
AcoI YGGCCR 2 cut(s) 81, 102
AcuI CTGAAG 1 cut(s) 159
AcvI CACGTG 1 cut(s) 74
AfaI GTAC 1 cut(s) 602
AfiI CCNNNNNNNGG 3 cut(s) 239, 330, 581
AflIII ACRYGT 3 cut(s) 71, 73, 466
AgsI TTSAA 3 cut(s) 214, 451, 635
AloI GAACNNNNNNTCC 2 cut(s) 243, 275
AluBI AGCT 4 cut(s) 119, 150, 435, 535
AluI AGCT 4 cut(s) 119, 150, 435, 535
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 1 cut(s) 512
Aor13HI TCCGGA 1 cut(s) 520
AoxI GGCC 2 cut(s) 81, 102
ApeKI GCWGC 1 cut(s) 27
AseI ATTAAT 1 cut(s) 557
AspS9I GGNCC 1 cut(s) 49
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 82
AvaII GGWCC 1 cut(s) 49
BaeGI GKGCMC 1 cut(s) 589
BanI GGYRCC 1 cut(s) 584
BbrPI CACGTG 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 39
BccI CCATC 4 cut(s) 29, 385, 477, 609
BciVI GTATCC 1 cut(s) 179
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 1 cut(s) 263
BfuI GTATCC 1 cut(s) 179
BisI GCNGC 1 cut(s) 28
BlsI GCNGC 1 cut(s) 29
Bme1390I CCNGG 1 cut(s) 80
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 3 cut(s) 130, 335, 586
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 1 cut(s) 487
BoxI GACNNNNGTC 1 cut(s) 74
BpiI GAAGAC 1 cut(s) 147
BpuEI CTTGAG 2 cut(s) 200, 457
BpuMI CCSGG 1 cut(s) 80
Bsa29I ATCGAT 1 cut(s) 606
BsaAI YACGTR 1 cut(s) 74
BsaWI WCCGGW 1 cut(s) 520
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bsc4I CCNNNNNNNGG 3 cut(s) 239, 330, 581
Bse1I ACTGG 2 cut(s) 382, 479
BseAI TCCGGA 1 cut(s) 520
BseCI ATCGAT 1 cut(s) 606
BseGI GGATG 6 cut(s) 231, 257, 396, 488, 502, 620
BseLI CCNNNNNNNGG 3 cut(s) 239, 330, 581
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 2 cut(s) 382, 479
BseRI GAGGAG 1 cut(s) 346
BseSI GKGCMC 1 cut(s) 589
BseXI GCAGC 1 cut(s) 39
BshFI GGCC 2 cut(s) 83, 104
BshNI GGYRCC 1 cut(s) 584
BshVI ATCGAT 1 cut(s) 606
BsiSI CCGG 4 cut(s) 80, 101, 263, 521
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 3 cut(s) 239, 330, 581
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 2 cut(s) 83, 104
Bsp1286I GDGCHC 1 cut(s) 589
Bsp13I TCCGGA 1 cut(s) 520
Bsp1407I TGTACA 1 cut(s) 600
Bsp143I GATC 1 cut(s) 517
BspANI GGCC 2 cut(s) 83, 104
BspCNI CTCAG 1 cut(s) 339
BspDI ATCGAT 1 cut(s) 606
BspEI TCCGGA 1 cut(s) 520
BspLI GGNNCC 3 cut(s) 130, 335, 586
BspPI GGATC 1 cut(s) 512
BspT107I GGYRCC 1 cut(s) 584
BsrGI TGTACA 1 cut(s) 600
BsrI ACTGG 2 cut(s) 382, 479
BssMI GATC 1 cut(s) 517
Bst4CI ACNGT 3 cut(s) 94, 146, 426
BstAUI TGTACA 1 cut(s) 600
BstBAI YACGTR 1 cut(s) 74
BstDEI CTNAG 1 cut(s) 347
BstF5I GGATG 6 cut(s) 231, 257, 396, 488, 502, 620
BstKTI GATC 1 cut(s) 520
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 1 cut(s) 517
BstMWI GCNNNNNNNGC 3 cut(s) 403, 452, 461
BstNSI RCATGY 1 cut(s) 470
BstPAI GACNNNNGTC 1 cut(s) 74
BstSCI CCNGG 1 cut(s) 78
BstSLI GKGCMC 1 cut(s) 589
BstV1I GCAGC 1 cut(s) 39
BstV2I GAAGAC 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 606
BsuI GTATCC 1 cut(s) 179
BsuRI GGCC 2 cut(s) 83, 104
BsuTUI ATCGAT 1 cut(s) 606
BtsCI GGATG 6 cut(s) 231, 257, 396, 488, 502, 620
BtsIMutI CAGTG 2 cut(s) 105, 422
Cfr13I GGNCC 1 cut(s) 49
ClaI ATCGAT 1 cut(s) 606
Csp6I GTAC 1 cut(s) 601
CviAII CATG 4 cut(s) 46, 158, 467, 492
CviQI GTAC 1 cut(s) 601
DdeI CTNAG 1 cut(s) 347
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
EaeI YGGCCR 2 cut(s) 81, 102
Eco47I GGWCC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 159
Eco72I CACGTG 1 cut(s) 74
EcoT22I ATGCAT 1 cut(s) 502
FaeI CATG 4 cut(s) 49, 161, 470, 495
FaiI YATR 7 cut(s) 8, 47, 159, 197, 468, 493, 507
FaqI GGGAC 1 cut(s) 62
FatI CATG 4 cut(s) 45, 157, 466, 491
FblI GTMKAC 1 cut(s) 471
Fnu4HI GCNGC 1 cut(s) 28
FokI GGATG 6 cut(s) 238, 264, 403, 495, 509, 627
Fsp4HI GCNGC 1 cut(s) 28
GluI GCNGC 1 cut(s) 28
HaeIII GGCC 2 cut(s) 83, 104
HapII CCGG 4 cut(s) 80, 101, 263, 521
Hin1II CATG 4 cut(s) 49, 161, 470, 495
HinfI GANTC 5 cut(s) 210, 245, 271, 528, 540
HpaII CCGG 4 cut(s) 80, 101, 263, 521
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 2 cut(s) 472, 601
Hpy188I TCNGA 6 cut(s) 69, 128, 139, 173, 209, 576
Hpy188III TCNNGA 4 cut(s) 179, 249, 323, 521
Hpy8I GTNNAC 2 cut(s) 472, 601
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 4 cut(s) 74, 518, 571, 605
HpyCH4III ACNGT 3 cut(s) 94, 146, 426
HpyCH4IV ACGT 3 cut(s) 73, 302, 513
HpyCH4V TGCA 3 cut(s) 414, 500, 554
HpyF10VI GCNNNNNNNGC 3 cut(s) 403, 452, 461
HpyF3I CTNAG 1 cut(s) 347
HpySE526I ACGT 3 cut(s) 73, 302, 513
Hsp92II CATG 4 cut(s) 49, 161, 470, 495
Kpn2I TCCGGA 1 cut(s) 520
Kzo9I GATC 1 cut(s) 517
LmnI GCTCC 3 cut(s) 128, 333, 394
Lsp1109I GCAGC 1 cut(s) 39
LweI GCATC 1 cut(s) 487
MaeII ACGT 3 cut(s) 73, 302, 513
MaeIII GTNAC 2 cut(s) 53, 94
MalI GATC 1 cut(s) 519
MboI GATC 1 cut(s) 517
MboII GAAGA 1 cut(s) 152
MhlI GDGCHC 1 cut(s) 589
MluCI AATT 2 cut(s) 558, 630
MlyI GAGTC 2 cut(s) 265, 534
MnlI CCTC 6 cut(s) 22, 145, 175, 177, 324, 574
Mph1103I ATGCAT 1 cut(s) 502
MroI TCCGGA 1 cut(s) 520
MseI TTAA 2 cut(s) 428, 557
MspI CCGG 4 cut(s) 80, 101, 263, 521
MspR9I CCNGG 1 cut(s) 80
Mva1269I GAATGC 1 cut(s) 397
MwoI GCNNNNNNNGC 3 cut(s) 403, 452, 461
NciI CCSGG 1 cut(s) 80
NdeII GATC 1 cut(s) 517
NlaIII CATG 4 cut(s) 49, 161, 470, 495
NlaIV GGNNCC 3 cut(s) 130, 335, 586
NmuCI GTSAC 2 cut(s) 53, 94
NsiI ATGCAT 1 cut(s) 502
NspI RCATGY 1 cut(s) 470
PciI ACATGT 1 cut(s) 466
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PctI GAATGC 1 cut(s) 397
PfeI GAWTC 3 cut(s) 210, 245, 528
PkrI GCNGC 1 cut(s) 29
PleI GAGTC 2 cut(s) 265, 534
PmaCI CACGTG 1 cut(s) 74
PmlI CACGTG 1 cut(s) 74
PpsI GAGTC 2 cut(s) 265, 534
Ppu21I YACGTR 1 cut(s) 74
PscI ACATGT 1 cut(s) 466
PshAI GACNNNNGTC 1 cut(s) 74
PshBI ATTAAT 1 cut(s) 557
PsiI TTATAA 1 cut(s) 507
PspCI CACGTG 1 cut(s) 74
PspN4I GGNNCC 3 cut(s) 130, 335, 586
PspPI GGNCC 1 cut(s) 49
RsaI GTAC 1 cut(s) 602
RsaNI GTAC 1 cut(s) 601
SaqAI TTAA 2 cut(s) 428, 557
SatI GCNGC 1 cut(s) 28
Sau3AI GATC 1 cut(s) 517
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 2 cut(s) 265, 534
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 1 cut(s) 589
SfaNI GCATC 1 cut(s) 487
SinI GGWCC 1 cut(s) 49
SmlI CTYRAG 2 cut(s) 179, 436
SmoI CTYRAG 2 cut(s) 179, 436
Sse9I AATT 2 cut(s) 558, 630
StyD4I CCNGG 1 cut(s) 78
TaaI ACNGT 3 cut(s) 94, 146, 426
TaiI ACGT 3 cut(s) 76, 305, 516
TaqI TCGA 3 cut(s) 305, 531, 606
TasI AATT 2 cut(s) 558, 630
TatI WGTACW 1 cut(s) 600
TfiI GAWTC 3 cut(s) 210, 245, 528
Tru1I TTAA 2 cut(s) 428, 557
Tru9I TTAA 2 cut(s) 428, 557
TscAI CASTG 2 cut(s) 112, 429
TseFI GTSAC 2 cut(s) 53, 94
TseI GCWGC 1 cut(s) 27
Tsp45I GTSAC 2 cut(s) 53, 94
TspDTI ATGAA 4 cut(s) 34, 112, 624, 654
TspGWI ACGGA 1 cut(s) 41
TspRI CASTG 2 cut(s) 112, 429
VpaK11BI GGWCC 1 cut(s) 49
VspI ATTAAT 1 cut(s) 557
XceI RCATGY 1 cut(s) 470
XmiI GTMKAC 1 cut(s) 471
Zsp2I ATGCAT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.