Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0000881

Physical Location & Seq

Genomic Coordinates Reverse (-)

9472399 .. 9476763

Gene Structure 4365 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1077800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2058 bp

ATGAAGGAGATGAACATTCTGAAAATGTGGGGTGGAGTGGAGCTGTCATTACTGCAAATACTCGGGCTGGAGAAACCACCCGCAATGAATACCAATCAAATGGATCAAATAAATTGGTGTTTAAAGCATTTGTGGAAAGAAAAAAAAAAAACAGAAGTCGAATTCCGAAAGAACCAAAAACAACTCCTTTTTTGTTGTGTGTATTCTGCTGTTCAGACCAATCAAGGCCAGTGCCTCGTTTACATGCATGCTGGAACCAATCCATTTGAAGTCATCACCCAAGCTTTGCCTTCTTTTCTCGATTGGTTTGGCTGGTGTACATGGGATACCTTTTACACTGATGTCACATCTGAGGGTGTGGAGCATGGCCTTGAAAGCTTATCGAAGGGAGGGATGCCTCCACGGTTCCTAATCATTGATGACGGTTGGCAACAGATTGAAAAGAAAGACAAGGATTCTAATGTTGTTGTACAAGAAGGAGCACAGTTTGCAAGTAGGTTGACAGGAATCAAAGAGAACGAAAAATTCCAAAAGAATGACCACAACAGTGAGCAAGTCTCTGGCCTGAAACACGTTGTGGATGTAGCCAAGCAACATAACAATGTGAAGTCTGTGTATGTATGGCATGCCCTAGCTGGTTACTGGGGTGGAGTGAAACCAGCAGCTACTGGCATGGAGCACCATGACACTGCTTTGGCATACCCAGTGACGTGCCCTGGTGTAGAGGGCAACCAACCAGACATAGTTATGGACAGCTTATCTGTTCATGGTCTCGGTCTGGTACATCCGAAGAAAGTTTTCAATTTCTACAATGAACTTCATTCCTACTTGGCTTCTTGTGGAGTTGATGGAGTTAAGGTTGATGTTCAGAACATCATTGAGACTCTAGGGGCTGGTCATGGTGGTAGAGTTTCTCTAACCCACAGCTACCACCAGGCACTTGAGGCTTCAACTGCTGTTGTCAGAGCTTCTGATGACTTATACCCCAGTGATCCTGCTTCACACACGATTCGTATATCTTCTGTTGCTTATAACACTCTTTTCCTTGGGGAATTCGTGCACCCTGACTGGGATATGTTTCATGTAAGCATGAAACGTCAATATGTCTGTTTGCTGAAGATTTACAATTCAATTTGTCTGATTATCTGCAATCATTTTGTGTTTGACATTCAGTGTCATTTAGTTGGAATAAAAGTTAATTTTGTATATCACGACATTGATGAAACAATCTTTACTTTGCAGAGTGTACATCCAGCGGCAGAGTATCACGGTGCAGCTCGTGCTGTTGGCGGATGTGCAATCTATGTCAGTGACAAACCAGGCAATCACAATTTTGACCTTCTGAGGAAGCTGGTCCTCCCTAATGGATCTGTCCTCCGTGCCAAGTTACCTGGCAGGCCTACCCGTGATTGTCTCTTTGCTGATCCAGCAAGAGACAGGACCAGCTTGCTCAAAATATGGAATGTCAACAATTGCTCTGGTGTGATTGGTGTGTTTAACTGTCAAGGTGCCGGCTGGTGCAAGCTTGAAAAGAAGACACGCATTCATGATAACTCTCCGGGTACCCTCAGCGGTTCTGTTTGTGCCACTGATGTTGATGCCATTGCTCAAGTTGCTGGAGCTGATTGGAATGGAGAAACAGTTGTTTATGCTCATAAGTCAGGAGAGGTTCTTCGGTTGCCAAAAGGTGCTTCAATGCCTGTGACGCTCAAAGTTCTTGAATATGAGGTTTTCCATTTCTGTCCTCTCAAGAAAATTACATCCGACGTCTCCTTTGCACCAATAGGCTTACTTGACATGTTCAATTCAAGCGCTGCTGTGGAGCAGGTTGACATCCATCTGGCTTCTGATAAGAACCAATCTCCCACTGCGACAATTGGTCTCAAAATCCGAGGATGTGGAAGGTTTGGAGCTTACTCTTCCCAGCGTCCGCTGAAGTGCACTGTTGACAATGCTGAGACCGACTTCGAATATGACTCTGCATCTGGATTAATGACGATTGCCATCCCGGTCCCAGAAAAAGAGATGTACAGATGGTCAGTAGAGATTCAAATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005911 GO:0005975 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009506 GO:0009628 GO:0015925 GO:0016052 GO:0016787 GO:0016798 GO:0030054 GO:0033530 GO:0034484 GO:0044238 GO:0050896 GO:0052692 GO:0055044 GO:0071704 GO:0080167 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00052 map00052

Pfam Domains

Protein Families

Raffinose_syn

Protein Analysis

686

Amino Acids

76.01

Weight (kDa)

6.27

Isoelectric Point (pI)

29.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Raffinose_syn	PF05691	58 - 318	1.7e-103	Raffinose synthase or seed imbibition protein Sip1
Raffinose_syn	PF05691	317 - 362	3.4e-14	Raffinose synthase or seed imbibition protein Sip1
Raffinose_syn	PF05691	410 - 668	2e-107	Raffinose synthase or seed imbibition protein Sip1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57520 AT3G57520 AT3G57520
fragaria_vesca	FvH4_3g00160 FvH4_6g23550 FvH4_6g23550 FvH4_6g23550 FvH4_6g23550
malus_domestica	MD09G1161700.v1.1 MD09G1162100.v1.1 MD10G1341400.v1.1 MD10G1341500.v1.1 MD10G1341600.v1.1 MD12G1077800.v1.1 MD14G1070300.v1.1
prunus_persica	Prupe.4G001700_v2.0.a1 Prupe.5G162400_v2.0.a1 Prupe.5G162400_v2.0.a1 Prupe.7G064000_v2.0.a1 Prupe.7G064000_v2.0.a1
pyrus_communis	pycom10g29090 pycom12g06210 pycom14g05800 pycom14g05880 pycom14g05890 pycom14g05910 pycom14g05930
rosa_chinensis	RchiOBHm_Chr3g0481621 RchiOBHm_Chr5g0000211 RchiOBHm_Chr7g0192011 RchiOBHm_Chr7g0192021
rosa_laevigata	RLG00000004409 RLG00000023385 RLG00000030850
rosa_multiflora	Rmu_co8093432.1_g000001 Rmu_co8314355.1_g000001 Rmu_sc0010409.1_g000002 Rmu_sc0021936.1_g000002 Rmu_ssc0000243.1_g000010 Rmu_ssc0000421.1_g000033
rosa_roxburghii	Rroxscaffold_1G00075890 Rroxscaffold_3G00263360 Rroxscaffold_6G00400310
rosa_rugosa	Rorug03G0192700 Rorug03G0192800 Rorug03G0192900 Rorug04G0382500 Rorug06G0512300 Rorug06G0512300
rosa_samantha	Rh3AG244100 Rh3BG279100 Rh3BG279300 Rh3CG274100 Rh3DG270900 Rh5AG001400 Rh5BG002800 Rh5CG001700 Rh5DG001600 Rh7AG122700 Rh7AG122800 Rh7BG124300 Rh7CG127000 Rh7DG125800
rosa_wichuraiana	Rw3G021800 Rw5G000190 Rw7G010450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1032
AatII	GACGTC	1 cut(s)	1770
Acc36I	ACCTGC	1 cut(s)	1816
Acc65I	GGTACC	1 cut(s)	1562
AccB1I	GGYRCC	2 cut(s)	1508, 1562
AciI	CCGC	5 cut(s)	81, 1256, 1290, 1572, 1931
AclWI	GGATC	4 cut(s)	111, 986, 1375, 1418
AcsI	RAATTY	3 cut(s)	161, 524, 1052
AcuI	CTGAAG	2 cut(s)	1136, 1955
AcyI	GRCGYC	1 cut(s)	1767
AdeI	CACNNNGTG	1 cut(s)	577
AfaI	GTAC	6 cut(s)	319, 471, 783, 1248, 1564, 2030
AfeI	AGCGCT	1 cut(s)	1813
AfiI	CCNNNNNNNGG	1 cut(s)	1069
AflIII	ACRYGT	2 cut(s)	571, 1797
AhdI	GACNNNNNGTC	1 cut(s)	1878
AjiI	CACGTC	1 cut(s)	711
AjnI	CCWGG	4 cut(s)	715, 933, 1318, 1390
AjuI	GAANNNNNNNTTGG	2 cut(s)	1675, 1707
AleI	CACNNNNGTG	1 cut(s)	546
Alw21I	GWGCWC	4 cut(s)	484, 681, 1062, 1943
Alw26I	GTCTC	8 cut(s)	562, 776, 875, 1418, 1428, 1774, 1886, 1952
Alw44I	GTGCAC	2 cut(s)	1058, 1939
AlwI	GGATC	4 cut(s)	111, 986, 1375, 1418
AlwNI	CAGNNNCTG	1 cut(s)	668
Ama87I	CYCGRG	1 cut(s)	62
Aor51HI	AGCGCT	1 cut(s)	1813
AoxI	GGCC	4 cut(s)	226, 367, 562, 1397
ApaLI	GTGCAC	2 cut(s)	1058, 1939
ApeKI	GCWGC	3 cut(s)	662, 1274, 1814
ApoI	RAATTY	3 cut(s)	161, 524, 1052
ArsI	GACNNNNNNTTYG	2 cut(s)	1757, 1789
AseI	ATTAAT	1 cut(s)	1991
Asp700I	GAANNNNTTC	1 cut(s)	797
Asp718I	GGTACC	1 cut(s)	1562
AspLEI	GCGC	1 cut(s)	1814
AspS9I	GGNCC	3 cut(s)	1354, 1440, 2011
AsuC2I	CCSGG	2 cut(s)	1560, 2009
AsuHPI	GGTGA	1 cut(s)	268
AsuII	TTCGAA	1 cut(s)	1968
AvaI	CYCGRG	1 cut(s)	62
AvaII	GGWCC	3 cut(s)	1354, 1440, 2011
BaeGI	GKGCMC	3 cut(s)	716, 1062, 1943
BanI	GGYRCC	2 cut(s)	1508, 1562
BauI	CACGAG	1 cut(s)	1278
BbsI	GAAGAC	1 cut(s)	1541
Bbv12I	GWGCWC	4 cut(s)	484, 681, 1062, 1943
BbvCI	CCTCAGC	1 cut(s)	1568
BbvI	GCAGC	3 cut(s)	674, 1286, 1801
BccI	CCATC	4 cut(s)	842, 1845, 2012, 2028
BciT130I	CCWGG	4 cut(s)	717, 935, 1320, 1392
BciVI	GTATCC	1 cut(s)	319
BcnI	CCSGG	2 cut(s)	1560, 2009
BcoDI	GTCTC	8 cut(s)	562, 776, 875, 1418, 1428, 1774, 1886, 1952
BfaI	CTAG	2 cut(s)	632, 887
BfoI	RGCGCY	1 cut(s)	1815
BfuAI	ACCTGC	1 cut(s)	1816
BfuI	GTATCC	1 cut(s)	319
BisI	GCNGC	4 cut(s)	663, 1257, 1275, 1815
BlsI	GCNGC	4 cut(s)	664, 1258, 1276, 1816
Bme1390I	CCNGG	6 cut(s)	717, 935, 1320, 1392, 1560, 2009
Bme18I	GGWCC	3 cut(s)	1354, 1440, 2011
BmeRI	GACNNNNNGTC	1 cut(s)	1878
BmeT110I	CYCGRG	1 cut(s)	62
BmgBI	CACGTC	1 cut(s)	711
BmgT120I	GGNCC	3 cut(s)	1354, 1440, 2011
BmiI	GGNNCC	5 cut(s)	256, 407, 1510, 1564, 2013
BmrFI	CCNGG	6 cut(s)	717, 935, 1320, 1392, 1560, 2009
BmrI	ACTGGG	4 cut(s)	652, 698, 981, 1078
BmsI	GCATC	3 cut(s)	384, 1588, 1991
BmuI	ACTGGG	4 cut(s)	652, 698, 981, 1078
BpiI	GAAGAC	1 cut(s)	1541
BplI	GAGNNNNNCTC	2 cut(s)	542, 574
BpmI	CTGGAG	2 cut(s)	89, 1638
Bpu10I	CCTNAGC	1 cut(s)	1568
Bpu14I	TTCGAA	1 cut(s)	1968
BpuEI	CTTGAG	3 cut(s)	962, 1593, 1733
BpuMI	CCSGG	2 cut(s)	1560, 2009
BsaBI	GATNNNNATC	1 cut(s)	2003
BsaHI	GRCGYC	1 cut(s)	1767
BsaI	GGTCTC	3 cut(s)	776, 1886, 1952
BsaJI	CCNNGG	4 cut(s)	401, 715, 1045, 1891
BsaXI	ACNNNNNCTCC	4 cut(s)	843, 873, 1626, 1656
Bsc4I	CCNNNNNNNGG	1 cut(s)	1069
Bse118I	RCCGGY	1 cut(s)	1511
Bse1I	ACTGG	6 cut(s)	229, 647, 673, 704, 987, 1073
Bse3DI	GCAATG	2 cut(s)	90, 1602
Bse8I	GATNNNNATC	1 cut(s)	2003
BseBI	CCWGG	4 cut(s)	717, 935, 1320, 1392
BseDI	CCNNGG	4 cut(s)	401, 715, 1045, 1891
BseGI	GGATG	9 cut(s)	399, 586, 784, 1249, 1298, 1760, 1833, 1901, 2004
BseJI	GATNNNNATC	1 cut(s)	2003
BseLI	CCNNNNNNNGG	1 cut(s)	1069
BseMI	GCAATG	2 cut(s)	90, 1602
BseMII	CTCAG	4 cut(s)	342, 1334, 1582, 1947
BseNI	ACTGG	6 cut(s)	229, 647, 673, 704, 987, 1073
BseSI	GKGCMC	3 cut(s)	716, 1062, 1943
BseXI	GCAGC	3 cut(s)	674, 1286, 1801
BseYI	CCCAGC	1 cut(s)	1923
BsgI	GTGCAG	1 cut(s)	1293
BshFI	GGCC	4 cut(s)	228, 369, 564, 1399
BshNI	GGYRCC	2 cut(s)	1508, 1562
BsiHKAI	GWGCWC	4 cut(s)	484, 681, 1062, 1943
BsiHKCI	CYCGRG	1 cut(s)	62
BsiSI	CCGG	3 cut(s)	1512, 1559, 2009
BslFI	GGGAC	1 cut(s)	1997
BslI	CCNNNNNNNGG	1 cut(s)	1069
BsmAI	GTCTC	8 cut(s)	562, 776, 875, 1418, 1428, 1774, 1886, 1952
BsmBI	CGTCTC	1 cut(s)	1774
BsmFI	GGGAC	1 cut(s)	1997
BsmI	GAATGC	1 cut(s)	1542
BsnI	GGCC	4 cut(s)	228, 369, 564, 1399
Bso31I	GGTCTC	3 cut(s)	776, 1886, 1952
BsoBI	CYCGRG	1 cut(s)	62
Bsp119I	TTCGAA	1 cut(s)	1968
Bsp1286I	GDGCHC	5 cut(s)	484, 681, 716, 1062, 1943
Bsp1407I	TGTACA	4 cut(s)	317, 469, 1246, 2028
Bsp143I	GATC	4 cut(s)	103, 991, 1367, 1423
BspACI	CCGC	5 cut(s)	81, 1256, 1290, 1572, 1931
BspANI	GGCC	4 cut(s)	228, 369, 564, 1399
BspCNI	CTCAG	4 cut(s)	343, 1335, 1581, 1948
BspHI	TCATGA	1 cut(s)	1546
BspLI	GGNNCC	5 cut(s)	256, 407, 1510, 1564, 2013
BspMI	ACCTGC	1 cut(s)	1816
BspPI	GGATC	4 cut(s)	111, 986, 1375, 1418
BspT104I	TTCGAA	1 cut(s)	1968
BspT107I	GGYRCC	2 cut(s)	1508, 1562
BspTNI	GGTCTC	3 cut(s)	776, 1886, 1952
BsrDI	GCAATG	2 cut(s)	90, 1602
BsrFI	RCCGGY	1 cut(s)	1511
BsrGI	TGTACA	4 cut(s)	317, 469, 1246, 2028
BsrI	ACTGG	6 cut(s)	229, 647, 673, 704, 987, 1073
BssAI	RCCGGY	1 cut(s)	1511
BssECI	CCNNGG	4 cut(s)	401, 715, 1045, 1891
BssMI	GATC	4 cut(s)	103, 991, 1367, 1423
BssNI	GRCGYC	1 cut(s)	1767
BssSI	CACGAG	1 cut(s)	1278
BssT1I	CCWWGG	1 cut(s)	1045
Bst2BI	CACGAG	1 cut(s)	1278
Bst2UI	CCWGG	4 cut(s)	717, 935, 1320, 1392
Bst4CI	ACNGT	8 cut(s)	405, 425, 486, 548, 1271, 1502, 1642, 1945
Bst6I	CTCTTC	1 cut(s)	1924
BstACI	GRCGYC	1 cut(s)	1767
BstAPI	GCANNNNNTGC	2 cut(s)	488, 1280
BstAUI	TGTACA	4 cut(s)	317, 469, 1246, 2028
BstBI	TTCGAA	1 cut(s)	1968
BstC8I	GCNNGC	6 cut(s)	249, 627, 1397, 1448, 1513, 1523
BstDEI	CTNAG	4 cut(s)	351, 1343, 1568, 1956
BstDSI	CCRYGG	1 cut(s)	401
BstF5I	GGATG	9 cut(s)	399, 586, 784, 1249, 1298, 1760, 1833, 1901, 2004
BstH2I	RGCGCY	1 cut(s)	1815
BstHHI	GCGC	1 cut(s)	1814
BstKTI	GATC	4 cut(s)	106, 994, 1370, 1426
BstMAI	GTCTC	8 cut(s)	562, 776, 875, 1418, 1428, 1774, 1886, 1952
BstMBI	GATC	4 cut(s)	103, 991, 1367, 1423
BstMWI	GCNNNNNNNGC	8 cut(s)	375, 488, 944, 953, 1280, 1427, 1613, 1705
BstNI	CCWGG	4 cut(s)	717, 935, 1320, 1392
BstNSI	RCATGY	4 cut(s)	247, 251, 629, 1801
BstSCI	CCNGG	6 cut(s)	715, 933, 1318, 1390, 1558, 2007
BstSLI	GKGCMC	3 cut(s)	716, 1062, 1943
BstV1I	GCAGC	3 cut(s)	674, 1286, 1801
BstV2I	GAAGAC	1 cut(s)	1541
BstX2I	RGATCY	1 cut(s)	1367
BstXI	CCANNNNNNTGG	1 cut(s)	100
BstYI	RGATCY	1 cut(s)	1367
BsuI	GTATCC	1 cut(s)	319
BsuRI	GGCC	4 cut(s)	228, 369, 564, 1399
BtgI	CCRYGG	1 cut(s)	401
BtrI	CACGTC	1 cut(s)	711
BtsCI	GGATG	9 cut(s)	399, 586, 784, 1249, 1298, 1760, 1833, 1901, 2004
BtsI	GCAGTG	2 cut(s)	687, 1866
BveI	ACCTGC	1 cut(s)	1816
Cac8I	GCNNGC	6 cut(s)	249, 627, 1397, 1448, 1513, 1523
CaiI	CAGNNNCTG	1 cut(s)	668
CciI	TCATGA	1 cut(s)	1546
CfoI	GCGC	1 cut(s)	1814
Cfr10I	RCCGGY	1 cut(s)	1511
Cfr13I	GGNCC	3 cut(s)	1354, 1440, 2011
CseI	GACGC	2 cut(s)	1714, 1916
Csp6I	GTAC	6 cut(s)	318, 470, 782, 1247, 1563, 2029
CspCI	CAANNNNNGTGG	2 cut(s)	1576, 1611
CviQI	GTAC	6 cut(s)	318, 470, 782, 1247, 1563, 2029
DdeI	CTNAG	4 cut(s)	351, 1343, 1568, 1956
DpnI	GATC	4 cut(s)	105, 993, 1369, 1425
DpnII	GATC	4 cut(s)	103, 991, 1367, 1423
DraI	TTTAAA	1 cut(s)	123
DraIII	CACNNNGTG	1 cut(s)	577
DriI	GACNNNNNGTC	1 cut(s)	1878
Eam1104I	CTCTTC	1 cut(s)	1924
Eam1105I	GACNNNNNGTC	1 cut(s)	1878
EarI	CTCTTC	1 cut(s)	1924
EciI	GGCGGA	1 cut(s)	1305
Eco130I	CCWWGG	1 cut(s)	1045
Eco147I	AGGCCT	1 cut(s)	1399
Eco31I	GGTCTC	3 cut(s)	776, 1886, 1952
Eco47I	GGWCC	3 cut(s)	1354, 1440, 2011
Eco47III	AGCGCT	1 cut(s)	1813
Eco57I	CTGAAG	2 cut(s)	1136, 1955
Eco88I	CYCGRG	1 cut(s)	62
EcoRI	GAATTC	2 cut(s)	161, 1052
EcoRII	CCWGG	4 cut(s)	715, 933, 1318, 1390
EcoT14I	CCWWGG	1 cut(s)	1045
EcoT22I	ATGCAT	1 cut(s)	249
ErhI	CCWWGG	1 cut(s)	1045
Esp3I	CGTCTC	1 cut(s)	1774
FaqI	GGGAC	1 cut(s)	1997
FauI	CCCGC	1 cut(s)	88
Fnu4HI	GCNGC	4 cut(s)	663, 1257, 1275, 1815
FokI	GGATG	9 cut(s)	406, 593, 771, 1236, 1305, 1747, 1820, 1908, 1991
Fsp4HI	GCNGC	4 cut(s)	663, 1257, 1275, 1815
FspBI	CTAG	2 cut(s)	632, 887
GlaI	GCGC	1 cut(s)	1813
GluI	GCNGC	4 cut(s)	663, 1257, 1275, 1815
GsaI	CCCAGC	1 cut(s)	1927
GsuI	CTGGAG	2 cut(s)	89, 1638
HaeII	RGCGCY	1 cut(s)	1815
HaeIII	GGCC	4 cut(s)	228, 369, 564, 1399
HapII	CCGG	3 cut(s)	1512, 1559, 2009
HgaI	GACGC	2 cut(s)	1714, 1916
HhaI	GCGC	1 cut(s)	1814
Hin1I	GRCGYC	1 cut(s)	1767
Hin6I	GCGC	1 cut(s)	1812
HinP1I	GCGC	1 cut(s)	1812
HincII	GTYRAC	4 cut(s)	501, 1468, 1831, 1948
HindII	GTYRAC	4 cut(s)	501, 1468, 1831, 1948
HindIII	AAGCTT	3 cut(s)	282, 376, 1523
HinfI	GANTC	6 cut(s)	455, 507, 883, 1009, 1976, 2047
HpaII	CCGG	3 cut(s)	1512, 1559, 2009
HphI	GGTGA	1 cut(s)	268
Hpy166II	GTNNAC	9 cut(s)	241, 318, 501, 1060, 1247, 1468, 1831, 1941, 1948
Hpy188III	TCNNGA	7 cut(s)	299, 1211, 1547, 1662, 1718, 1750, 1986
Hpy8I	GTNNAC	9 cut(s)	241, 318, 501, 1060, 1247, 1468, 1831, 1941, 1948
Hpy99I	CGWCG	1 cut(s)	1769
HpyAV	CCTTC	5 cut(s)	300, 379, 470, 1349, 1896
HpyCH4III	ACNGT	8 cut(s)	405, 425, 486, 548, 1271, 1502, 1642, 1945
HpyCH4IV	ACGT	4 cut(s)	573, 710, 1096, 1767
HpyF10VI	GCNNNNNNNGC	8 cut(s)	375, 488, 944, 953, 1280, 1427, 1613, 1705
HpyF3I	CTNAG	4 cut(s)	351, 1343, 1568, 1956
HpySE526I	ACGT	4 cut(s)	573, 710, 1096, 1767
Hsp92I	GRCGYC	1 cut(s)	1767
HspAI	GCGC	1 cut(s)	1812
KpnI	GGTACC	1 cut(s)	1566
KroI	GCCGGC	1 cut(s)	1511
KroNI	GCCGGC	1 cut(s)	1513
Kzo9I	GATC	4 cut(s)	103, 991, 1367, 1423
LmnI	GCTCC	7 cut(s)	40, 361, 479, 676, 1619, 1822, 1910
Lsp1109I	GCAGC	3 cut(s)	674, 1286, 1801
LweI	GCATC	3 cut(s)	384, 1588, 1991
MaeI	CTAG	2 cut(s)	632, 887
MaeII	ACGT	4 cut(s)	573, 710, 1096, 1767
MaeIII	GTNAC	6 cut(s)	343, 638, 706, 1310, 1386, 1702
MalI	GATC	4 cut(s)	105, 993, 1369, 1425
MboI	GATC	4 cut(s)	103, 991, 1367, 1423
MboII	GAAGA	6 cut(s)	802, 1011, 1129, 1546, 1664, 1911
MfeI	CAATTG	2 cut(s)	1471, 1875
MflI	RGATCY	1 cut(s)	1367
MhlI	GDGCHC	5 cut(s)	484, 681, 716, 1062, 1943
MlyI	GAGTC	2 cut(s)	877, 1970
MmeI	TCCRAC	2 cut(s)	1165, 1788
Mph1103I	ATGCAT	1 cut(s)	249
MroNI	GCCGGC	1 cut(s)	1511
MroXI	GAANNNNTTC	1 cut(s)	797
MseI	TTAA	5 cut(s)	122, 855, 1197, 1497, 1991
MslI	CAYNNNNRTG	3 cut(s)	546, 600, 746
MspA1I	CMGCKG	3 cut(s)	1256, 1572, 1933
MspI	CCGG	3 cut(s)	1512, 1559, 2009
MspR9I	CCNGG	6 cut(s)	717, 935, 1320, 1392, 1560, 2009
MunI	CAATTG	2 cut(s)	1471, 1875
Mva1269I	GAATGC	1 cut(s)	1542
MvaI	CCWGG	4 cut(s)	717, 935, 1320, 1392
MwoI	GCNNNNNNNGC	8 cut(s)	375, 488, 944, 953, 1280, 1427, 1613, 1705
NaeI	GCCGGC	1 cut(s)	1513
NciI	CCSGG	2 cut(s)	1560, 2009
NdeII	GATC	4 cut(s)	103, 991, 1367, 1423
NgoMIV	GCCGGC	1 cut(s)	1511
NlaIV	GGNNCC	5 cut(s)	256, 407, 1510, 1564, 2013
NmuCI	GTSAC	4 cut(s)	343, 706, 1310, 1702
NsiI	ATGCAT	1 cut(s)	249
NspI	RCATGY	4 cut(s)	247, 251, 629, 1801
NspV	TTCGAA	1 cut(s)	1968
OliI	CACNNNNGTG	1 cut(s)	546
PaeI	GCATGC	2 cut(s)	251, 629
PagI	TCATGA	1 cut(s)	1546
PceI	AGGCCT	1 cut(s)	1399
PciI	ACATGT	1 cut(s)	1797
PctI	GAATGC	1 cut(s)	1542
PdiI	GCCGGC	1 cut(s)	1513
PdmI	GAANNNNTTC	1 cut(s)	797
PfeI	GAWTC	4 cut(s)	455, 507, 1009, 2047
PkrI	GCNGC	4 cut(s)	664, 1258, 1276, 1816
PleI	GAGTC	2 cut(s)	877, 1970
PpsI	GAGTC	2 cut(s)	877, 1970
PscI	ACATGT	1 cut(s)	1797
PshBI	ATTAAT	1 cut(s)	1991
PsiI	TTATAA	1 cut(s)	1032
Psp6I	CCWGG	4 cut(s)	715, 933, 1318, 1390
PspFI	CCCAGC	1 cut(s)	1923
PspGI	CCWGG	4 cut(s)	715, 933, 1318, 1390
PspN4I	GGNNCC	5 cut(s)	256, 407, 1510, 1564, 2013
PspPI	GGNCC	3 cut(s)	1354, 1440, 2011
PstNI	CAGNNNCTG	1 cut(s)	668
PsuI	RGATCY	1 cut(s)	1367
RsaI	GTAC	6 cut(s)	319, 471, 783, 1248, 1564, 2030
RsaNI	GTAC	6 cut(s)	318, 470, 782, 1247, 1563, 2029
RseI	CAYNNNNRTG	3 cut(s)	546, 600, 746
SaqAI	TTAA	5 cut(s)	122, 855, 1197, 1497, 1991
SatI	GCNGC	4 cut(s)	663, 1257, 1275, 1815
Sau3AI	GATC	4 cut(s)	103, 991, 1367, 1423
Sau96I	GGNCC	3 cut(s)	1354, 1440, 2011
SchI	GAGTC	2 cut(s)	877, 1970
ScrFI	CCNGG	6 cut(s)	717, 935, 1320, 1392, 1560, 2009
SduI	GDGCHC	5 cut(s)	484, 681, 716, 1062, 1943
SfaNI	GCATC	3 cut(s)	384, 1588, 1991
SfuI	TTCGAA	1 cut(s)	1968
SinI	GGWCC	3 cut(s)	1354, 1440, 2011
SmiMI	CAYNNNNRTG	3 cut(s)	546, 600, 746
SmlI	CTYRAG	3 cut(s)	941, 1608, 1748
SmoI	CTYRAG	3 cut(s)	941, 1608, 1748
SphI	GCATGC	2 cut(s)	251, 629
SseBI	AGGCCT	1 cut(s)	1399
SsiI	CCGC	5 cut(s)	81, 1256, 1290, 1572, 1931
SspMI	CTAG	2 cut(s)	632, 887
StuI	AGGCCT	1 cut(s)	1399
StyD4I	CCNGG	6 cut(s)	715, 933, 1318, 1390, 1558, 2007
StyI	CCWWGG	1 cut(s)	1045
TaaI	ACNGT	8 cut(s)	405, 425, 486, 548, 1271, 1502, 1642, 1945
TaiI	ACGT	4 cut(s)	576, 713, 1099, 1770
TaqI	TCGA	4 cut(s)	159, 300, 383, 1968
TaqII	GACCGA	2 cut(s)	764, 1976
TatI	WGTACW	4 cut(s)	317, 469, 1246, 2028
TauI	GCSGC	1 cut(s)	1259
TfiI	GAWTC	4 cut(s)	455, 507, 1009, 2047
Tru1I	TTAA	5 cut(s)	122, 855, 1197, 1497, 1991
Tru9I	TTAA	5 cut(s)	122, 855, 1197, 1497, 1991
TseFI	GTSAC	4 cut(s)	343, 706, 1310, 1702
TseI	GCWGC	3 cut(s)	662, 1274, 1814
Tsp45I	GTSAC	4 cut(s)	343, 706, 1310, 1702
TspGWI	ACGGA	1 cut(s)	1367
VneI	GTGCAC	2 cut(s)	1058, 1939
VpaK11BI	GGWCC	3 cut(s)	1354, 1440, 2011
VspI	ATTAAT	1 cut(s)	1991
XapI	RAATTY	3 cut(s)	161, 524, 1052
XceI	RCATGY	4 cut(s)	247, 251, 629, 1801
XmnI	GAANNNNTTC	1 cut(s)	797
XspI	CTAG	2 cut(s)	632, 887
ZraI	GACGTC	1 cut(s)	1768
Zsp2I	ATGCAT	1 cut(s)	249

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.