MD10G1341600.v1.1

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000881
Physical Location & Seq
Forward (+)
41647748 .. 41649898
2151 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1341600.v1.1.491

Sequence Viewer

Length: 963 bp
ATGTCTACTTTTATGTGGGCTAAGTGCAGTTCAAAAAGAAGTGCTATTACTCGAGCATCTGATGATTACTACCCGAAAAACCCAACAACACAGACACTGCACATAGCTGCTGTGGCTTTCAACAGCATATTCCTTGGTGAAGTTGTTCTCCCAGATTGGGACATGTTCTATAGCCGTCATGAAGCAGCTGAGTTCCATGCTGCTGCTAGAGCTGTGGGAGGATGCGGAGTCTATGTTAGTGACAAACCTGGCCATCATGATTTCGAGATACTTAAAAGGCTTGTACTTCCTGATGGATCAATTCTGCGAGCTAGATTCCCCGGGAGGCCATCACGGGATTGTTTATTTGTCGACCCCGTTACAGATGGGAAGAGCCTTCTAAAGATATGGAATTTGAACAAATACAATGGAGTTTTAGGCATTTTCAATTGCCAAGGAGCAGGGAACTGGCCTTGTCTAGAACAAGTTGTTCCGGTAGAAGTTAGTGCTGAAGTGTCTGGGAAGGTGACCCCTGCAGATATTGAGTATTTCGACGAGGTTTCTGGCAAGCTGTGGACAGGAGATTGTGCGGTCTATTCCTTCGCAAAAGGGCGTCTGTCTCGGTTACCAAAGGATAAGTCATTTGACGTTGCATTAAGAGTTCTCCAATGCGATGTCTTTACTGTATCTCCTATTAAGGTTTACAAGCAAAACATTGAATTTGCAGCGATTGGATTATTGAACATGTACAATTCCGGTGGAGCTGTTGAAGCAGTTGAGTGCTTTAGTCATAACTCTAGCAGTGAAATACACATTAAGGGAAGAGGAGGTGGTAGCTTTGGCGCTTACTCTAGTTTAAAGCCCAAAGCTTGCTCAGTGAACTCCAAAGACGACGAGGAATTTGAATTTAGAGATGAAGACGACAATCTTTTAAAAGTAACAGTTCCTGCCACAACTAGTTGGAAAATTGTTCTACATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

321

Amino Acids

35.33

Weight (kDa)

6.25

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 9 - 304 5e-106 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 5, 351
AciI CCGC 2 cut(s) 225, 569
AclWI GGATC 1 cut(s) 304
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 4 cut(s) 391, 698, 878, 884
AcuI CTGAAG 1 cut(s) 510
AcyI GRCGYC 1 cut(s) 592
AfaI GTAC 2 cut(s) 285, 728
AfiI CCNNNNNNNGG 1 cut(s) 157
AflIII ACRYGT 2 cut(s) 162, 723
AgsI TTSAA 8 cut(s) 33, 121, 397, 427, 698, 721, 749, 884
AhlI ACTAGT 1 cut(s) 935
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 8 cut(s) 107, 188, 212, 311, 550, 743, 816, 848
AluI AGCT 8 cut(s) 107, 188, 212, 311, 550, 743, 816, 848
Alw26I GTCTC 1 cut(s) 603
AlwI GGATC 1 cut(s) 304
AlwNI CAGNNNCTG 2 cut(s) 97, 926
Ama87I CYCGRG 2 cut(s) 51, 320
AoxI GGCC 3 cut(s) 250, 326, 449
ApeKI GCWGC 5 cut(s) 107, 185, 200, 203, 704
ApoI RAATTY 4 cut(s) 391, 698, 878, 884
ArsI GACNNNNNNTTYG 4 cut(s) 602, 634, 863, 895
Asp700I GAANNNNTTC 1 cut(s) 144
AspLEI GCGC 1 cut(s) 824
AsuC2I CCSGG 2 cut(s) 321, 322
AsuHPI GGTGA 2 cut(s) 149, 517
AvaI CYCGRG 2 cut(s) 51, 320
BalI TGGCCA 1 cut(s) 252
BarI GAAGNNNNNNTAC 2 cut(s) 31, 63
BbsI GAAGAC 1 cut(s) 903
BbvI GCAGC 5 cut(s) 94, 187, 190, 197, 716
BccI CCATC 4 cut(s) 261, 287, 337, 359
BceAI ACGGC 1 cut(s) 159
BciT130I CCWGG 1 cut(s) 249
BcnI CCSGG 2 cut(s) 321, 322
BcoDI GTCTC 1 cut(s) 603
BcuI ACTAGT 1 cut(s) 935
BfaI CTAG 6 cut(s) 207, 312, 458, 777, 831, 936
BfmI CTRYAG 2 cut(s) 169, 513
BfoI RGCGCY 1 cut(s) 825
BisI GCNGC 5 cut(s) 108, 186, 201, 204, 705
BlsI GCNGC 5 cut(s) 109, 187, 202, 205, 706
Bme1390I CCNGG 3 cut(s) 249, 321, 322
BmeT110I CYCGRG 2 cut(s) 51, 320
BmrFI CCNGG 3 cut(s) 249, 321, 322
BmsI GCATC 2 cut(s) 65, 212
BpiI GAAGAC 1 cut(s) 903
BpuMI CCSGG 2 cut(s) 321, 322
BsaHI GRCGYC 1 cut(s) 592
BsaJI CCNNGG 4 cut(s) 133, 319, 320, 433
BsaWI WCCGGW 2 cut(s) 472, 734
BsaXI ACNNNNNCTCC 4 cut(s) 219, 249, 652, 682
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 452
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 4 cut(s) 133, 319, 320, 433
BseGI GGATG 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMII CTCAG 2 cut(s) 180, 867
BseNI ACTGG 1 cut(s) 452
BseRI GAGGAG 1 cut(s) 819
BseXI GCAGC 5 cut(s) 94, 187, 190, 197, 716
BsgI GTGCAG 2 cut(s) 46, 83
BshFI GGCC 3 cut(s) 252, 328, 451
BsiHKCI CYCGRG 2 cut(s) 51, 320
BsiSI CCGG 3 cut(s) 321, 473, 735
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 1 cut(s) 603
BsmFI GGGAC 1 cut(s) 173
BsnI GGCC 3 cut(s) 252, 328, 451
BsoBI CYCGRG 2 cut(s) 51, 320
Bsp1407I TGTACA 1 cut(s) 726
Bsp143I GATC 1 cut(s) 296
BspACI CCGC 2 cut(s) 225, 569
BspANI GGCC 3 cut(s) 252, 328, 451
BspCNI CTCAG 2 cut(s) 181, 866
BspHI TCATGA 2 cut(s) 178, 256
BspMAI CTGCAG 1 cut(s) 517
BspPI GGATC 1 cut(s) 304
BspQI GCTCTTC 1 cut(s) 365
BsrGI TGTACA 1 cut(s) 726
BsrI ACTGG 1 cut(s) 452
BssECI CCNNGG 4 cut(s) 133, 319, 320, 433
BssMI GATC 1 cut(s) 296
BssNI GRCGYC 1 cut(s) 592
BssT1I CCWWGG 2 cut(s) 133, 433
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 664, 922
Bst6I CTCTTC 2 cut(s) 365, 796
BstACI GRCGYC 1 cut(s) 592
BstAUI TGTACA 1 cut(s) 726
BstC8I GCNNGC 3 cut(s) 309, 548, 850
BstDEI CTNAG 3 cut(s) 21, 189, 853
BstEII GGTNACC 2 cut(s) 505, 603
BstF5I GGATG 1 cut(s) 227
BstH2I RGCGCY 1 cut(s) 825
BstHHI GCGC 1 cut(s) 824
BstKTI GATC 1 cut(s) 299
BstMAI GTCTC 1 cut(s) 603
BstMBI GATC 1 cut(s) 296
BstMWI GCNNNNNNNGC 3 cut(s) 113, 209, 749
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 2 cut(s) 166, 727
BstPI GGTNACC 2 cut(s) 505, 603
BstSCI CCNGG 3 cut(s) 247, 319, 320
BstSFI CTRYAG 2 cut(s) 169, 513
BstV1I GCAGC 5 cut(s) 94, 187, 190, 197, 716
BstV2I GAAGAC 1 cut(s) 903
BsuRI GGCC 3 cut(s) 252, 328, 451
BtgZI GCGATG 1 cut(s) 666
BtsCI GGATG 1 cut(s) 227
BtsI GCAGTG 2 cut(s) 95, 787
BtsIMutI CAGTG 3 cut(s) 95, 787, 861
Cac8I GCNNGC 3 cut(s) 309, 548, 850
CaiI CAGNNNCTG 2 cut(s) 97, 926
CciI TCATGA 2 cut(s) 178, 256
CfoI GCGC 1 cut(s) 824
Cfr9I CCCGGG 1 cut(s) 320
CseI GACGC 1 cut(s) 581
Csp6I GTAC 2 cut(s) 284, 727
CspCI CAANNNNNGTGG 2 cut(s) 718, 753
CviAII CATG 5 cut(s) 163, 179, 197, 257, 724
CviQI GTAC 2 cut(s) 284, 727
DdeI CTNAG 3 cut(s) 21, 189, 853
DpnI GATC 1 cut(s) 298
DpnII GATC 1 cut(s) 296
DraI TTTAAA 2 cut(s) 837, 912
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 2 cut(s) 365, 796
EarI CTCTTC 2 cut(s) 365, 796
Eco130I CCWWGG 2 cut(s) 133, 433
Eco57I CTGAAG 1 cut(s) 510
Eco88I CYCGRG 2 cut(s) 51, 320
Eco91I GGTNACC 2 cut(s) 505, 603
EcoO65I GGTNACC 2 cut(s) 505, 603
EcoRII CCWGG 1 cut(s) 247
EcoT14I CCWWGG 2 cut(s) 133, 433
ErhI CCWWGG 2 cut(s) 133, 433
FaeI CATG 5 cut(s) 166, 182, 200, 260, 727
FaqI GGGAC 1 cut(s) 173
FatI CATG 5 cut(s) 162, 178, 196, 256, 723
FblI GTMKAC 2 cut(s) 5, 351
Fnu4HI GCNGC 5 cut(s) 108, 186, 201, 204, 705
FokI GGATG 1 cut(s) 234
Fsp4HI GCNGC 5 cut(s) 108, 186, 201, 204, 705
FspBI CTAG 6 cut(s) 207, 312, 458, 777, 831, 936
GlaI GCGC 1 cut(s) 823
GluI GCNGC 5 cut(s) 108, 186, 201, 204, 705
HaeII RGCGCY 1 cut(s) 825
HaeIII GGCC 3 cut(s) 252, 328, 451
HapII CCGG 3 cut(s) 321, 473, 735
HgaI GACGC 1 cut(s) 581
HhaI GCGC 1 cut(s) 824
Hin1I GRCGYC 1 cut(s) 592
Hin1II CATG 5 cut(s) 166, 182, 200, 260, 727
Hin6I GCGC 1 cut(s) 822
HinP1I GCGC 1 cut(s) 822
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HindIII AAGCTT 1 cut(s) 846
HinfI GANTC 2 cut(s) 228, 315
HpaII CCGG 3 cut(s) 321, 473, 735
HphI GGTGA 2 cut(s) 149, 517
Hpy166II GTNNAC 5 cut(s) 6, 352, 555, 682, 859
Hpy188I TCNGA 1 cut(s) 61
Hpy188III TCNNGA 5 cut(s) 179, 257, 265, 290, 458
Hpy8I GTNNAC 5 cut(s) 6, 352, 555, 682, 859
Hpy99I CGWCG 2 cut(s) 536, 875
HpyAV CCTTC 3 cut(s) 386, 496, 589
HpyCH4III ACNGT 2 cut(s) 664, 922
HpyCH4IV ACGT 1 cut(s) 627
HpyCH4V TGCA 5 cut(s) 27, 100, 515, 632, 704
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 209, 749
HpyF3I CTNAG 3 cut(s) 21, 189, 853
HpySE526I ACGT 1 cut(s) 627
Hsp92I GRCGYC 1 cut(s) 592
Hsp92II CATG 5 cut(s) 166, 182, 200, 260, 727
HspAI GCGC 1 cut(s) 822
Kzo9I GATC 1 cut(s) 296
LguI GCTCTTC 1 cut(s) 365
LmnI GCTCC 2 cut(s) 437, 740
Lsp1109I GCAGC 5 cut(s) 94, 187, 190, 197, 716
LweI GCATC 2 cut(s) 65, 212
MaeI CTAG 6 cut(s) 207, 312, 458, 777, 831, 936
MaeII ACGT 1 cut(s) 627
MaeIII GTNAC 5 cut(s) 239, 358, 505, 603, 916
MalI GATC 1 cut(s) 298
MboI GATC 1 cut(s) 296
MboII GAAGA 3 cut(s) 382, 813, 908
MfeI CAATTG 1 cut(s) 427
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 8 cut(s) 300, 391, 427, 698, 730, 878, 884, 945
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 1 cut(s) 237
MmeI TCCRAC 1 cut(s) 920
MnlI CCTC 6 cut(s) 212, 318, 529, 797, 800, 868
Mox20I TGGCCA 1 cut(s) 252
MroXI GAANNNNTTC 1 cut(s) 144
MscI TGGCCA 1 cut(s) 252
MseI TTAA 6 cut(s) 273, 635, 675, 795, 836, 911
Msp20I TGGCCA 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 3 cut(s) 321, 473, 735
MspR9I CCNGG 3 cut(s) 249, 321, 322
MunI CAATTG 1 cut(s) 427
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 3 cut(s) 113, 209, 749
NciI CCSGG 2 cut(s) 321, 322
NdeII GATC 1 cut(s) 296
NlaIII CATG 5 cut(s) 166, 182, 200, 260, 727
NmuCI GTSAC 2 cut(s) 239, 505
NspI RCATGY 2 cut(s) 166, 727
PaeR7I CTCGAG 1 cut(s) 51
PagI TCATGA 2 cut(s) 178, 256
PciI ACATGT 2 cut(s) 162, 723
PciSI GCTCTTC 1 cut(s) 365
PdmI GAANNNNTTC 1 cut(s) 144
PfeI GAWTC 1 cut(s) 315
PkrI GCNGC 5 cut(s) 109, 187, 202, 205, 706
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
PscI ACATGT 2 cut(s) 162, 723
Psp6I CCWGG 1 cut(s) 247
PspEI GGTNACC 2 cut(s) 505, 603
PspGI CCWGG 1 cut(s) 247
PspXI VCTCGAGB 1 cut(s) 51
PstI CTGCAG 1 cut(s) 517
PstNI CAGNNNCTG 2 cut(s) 97, 926
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 2 cut(s) 285, 728
RsaNI GTAC 2 cut(s) 284, 727
SalI GTCGAC 1 cut(s) 350
SapI GCTCTTC 1 cut(s) 365
SaqAI TTAA 6 cut(s) 273, 635, 675, 795, 836, 911
SatI GCNGC 5 cut(s) 108, 186, 201, 204, 705
Sau3AI GATC 1 cut(s) 296
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 3 cut(s) 249, 321, 322
SfaNI GCATC 2 cut(s) 65, 212
SfcI CTRYAG 2 cut(s) 169, 513
Sfr274I CTCGAG 1 cut(s) 51
SlaI CTCGAG 1 cut(s) 51
SmaI CCCGGG 1 cut(s) 322
SmlI CTYRAG 1 cut(s) 51
SmoI CTYRAG 1 cut(s) 51
SpeI ACTAGT 1 cut(s) 935
Sse9I AATT 8 cut(s) 300, 391, 427, 698, 730, 878, 884, 945
SsiI CCGC 2 cut(s) 225, 569
SspMI CTAG 6 cut(s) 207, 312, 458, 777, 831, 936
StyD4I CCNGG 3 cut(s) 247, 319, 320
StyI CCWWGG 2 cut(s) 133, 433
TaaI ACNGT 2 cut(s) 664, 922
TaiI ACGT 1 cut(s) 630
TaqI TCGA 4 cut(s) 52, 264, 351, 531
TasI AATT 8 cut(s) 300, 391, 427, 698, 730, 878, 884, 945
TatI WGTACW 2 cut(s) 283, 726
TfiI GAWTC 1 cut(s) 315
Tru1I TTAA 6 cut(s) 273, 635, 675, 795, 836, 911
Tru9I TTAA 6 cut(s) 273, 635, 675, 795, 836, 911
TscAI CASTG 3 cut(s) 102, 787, 861
TseFI GTSAC 2 cut(s) 239, 505
TseI GCWGC 5 cut(s) 107, 185, 200, 203, 704
Tsp45I GTSAC 2 cut(s) 239, 505
TspDTI ATGAA 2 cut(s) 195, 909
TspMI CCCGGG 1 cut(s) 320
TspRI CASTG 3 cut(s) 102, 787, 861
XapI RAATTY 4 cut(s) 391, 698, 878, 884
XbaI TCTAGA 1 cut(s) 457
XceI RCATGY 2 cut(s) 166, 727
XhoI CTCGAG 1 cut(s) 51
XmaI CCCGGG 1 cut(s) 320
XmiI GTMKAC 2 cut(s) 5, 351
XmnI GAANNNNTTC 1 cut(s) 144
XspI CTAG 6 cut(s) 207, 312, 458, 777, 831, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.