Prupe.5G162400_v2.0.a1

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000881
Physical Location & Seq
Reverse (-)
14228419 .. 14231181
2763 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G162400.1

Sequence Viewer

Length: 1440 bp
ATGATTGTCCCAAGCATCAAAGATGACTGCCTCATGGTCCGGGACAATGTTGTTCTGTCTCATGTTCCAAGAAACATAAGTGTCGCACCAGCTGCCGATGAGGCTGCTTTCATTGGAGCCGCTTCTTCAAACTCTAGCTTTCGTCATGTCTTCTCTTTTGGGGTTCTTGAGTATAATAATTTTATGTGTCTCTTCAGATTCAAGCTCTGGTGGATGATACCGAGTTTCGGAGATTCGGGCTGTGAAGTTCCAGCAGAAACTCAAATGCTCCTCTTGGAAACAAGAGAAAAATCCACTGTCCAAAATGGGCTATCTGAGCCAACAACTGAGAACAGTTTGTACATTCTGTTATTATCAGTCTTGGATGGACCATTCAGGGCTAGTTTGCAAGGGAACACTGTAAATGAGCTTGAATTTTGCATCGAAAGCGGAGATCCCAACGTCCAAACCTCCCAAGTAACAGAAGCAATTTTTATGAATTTAGGAGACAACCCTTTTAAGCTCATTAGGAATTCCACAAAGTATATTTACACCTGGGATGCATTTTATCATAAAGTTGATCCTGAAGGGATTGAGAAGGGGCTTAAAAGTCTTTCAGAAGGAGGCTTCCCCACAAAATTTCTTATCATTGATGAAGGATGGCAGAACAAAGTAATGGAAGTTGAAGCTGAAGCTGATGAGACTGATTCATCTTATCGTGCCGCGTCTGTGGACAGATTGACTAGCATAGAAGAAAATGATAAGTTCAAGGGCTTCCGGTCAGGAAAGTCATATGCTAATCTCCGTGAGTTTGTAAAGTTCATCGAAGAGGAATATGGACTGAAATTAGTATATGCGTGCCACGCTTTAATTGGTTCGTGGGGAGGGGTACTTCCAACATCTGAAGAAATGAGGAAATACGACCCCTGGATCAAACACATAGTTCAGTCCCCTGGAAACGTGAGTCACGTTATCTGTACAACCCTTGGCCCCATGGAGAAATATGGGATTGGCATGATTGCCCCCTCAAATATTTATAGGTTTTATGACGATCTTCATAGCTACCTTGCAAGTTGCAATGTAGATGGAGTTAAGGTGGATGTGCAGAATGTACTGGAACTTCTTGGCTCTTGTTATGGAGGCCGTGAAGCATTGATGAGGCGGTACCAAGAAGCCCTTGAGGCATCTGTTATAAGAAACTTCCATCGCAATAACCTTATTTGGGGCATGAGCCTTAGCAATGACCACATTTACAGGATATCAGAGGATTTCATGCAAATGGAGCCAACATTTCAGACCCTACATGTTGCTGCTGTAGCTTTTAACAGTCTTCTCATGGGAGAGATTGCTGTTCCAGATTGGGACACGTTCTTTAGTGACCATTACACGGCTGAGTTCCATGCTGCAGCAAGGGCATTGGGGGGATGTCCAGTATATGTACAACTCAGGAGGAACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

480

Amino Acids

54.02

Weight (kDa)

5.07

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1172
Acc65I GGTACC 1 cut(s) 1143
AccB1I GGYRCC 1 cut(s) 1143
AccII CGCG 1 cut(s) 704
AciI CCGC 4 cut(s) 120, 429, 702, 1141
AclWI GGATC 3 cut(s) 428, 554, 917
AcsI RAATTY 4 cut(s) 413, 478, 511, 617
AcuI CTGAAG 4 cut(s) 178, 585, 690, 903
AfaI GTAC 6 cut(s) 341, 870, 958, 1092, 1145, 1419
AfiI CCNNNNNNNGG 3 cut(s) 227, 1201, 1366
AflIII ACRYGT 2 cut(s) 1282, 1344
AgsI TTSAA 5 cut(s) 129, 202, 413, 665, 748
AjnI CCWGG 3 cut(s) 533, 905, 931
AluBI AGCT 9 cut(s) 92, 138, 205, 409, 502, 668, 674, 1041, 1298
AluI AGCT 9 cut(s) 92, 138, 205, 409, 502, 668, 674, 1041, 1298
Alw26I GTCTC 4 cut(s) 63, 194, 480, 674
AlwI GGATC 3 cut(s) 428, 554, 917
AoxI GGCC 2 cut(s) 967, 1120
ApeKI GCWGC 5 cut(s) 92, 104, 1289, 1382, 1385
ApoI RAATTY 4 cut(s) 413, 478, 511, 617
Asp718I GGTACC 1 cut(s) 1143
AspS9I GGNCC 3 cut(s) 37, 368, 968
AsuC2I CCSGG 1 cut(s) 41
AvaII GGWCC 2 cut(s) 37, 368
BanI GGYRCC 1 cut(s) 1143
BarI GAAGNNNNNNTAC 2 cut(s) 1083, 1115
BbsI GAAGAC 2 cut(s) 142, 1301
BbvI GCAGC 5 cut(s) 79, 91, 1276, 1369, 1397
BccI CCATC 4 cut(s) 359, 633, 1058, 1191
BceAI ACGGC 2 cut(s) 1107, 1383
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 3 cut(s) 535, 907, 933
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 4 cut(s) 63, 194, 480, 674
BfaI CTAG 3 cut(s) 135, 381, 723
BfmI CTRYAG 2 cut(s) 1293, 1383
BglI GCCNNNNNGGC 2 cut(s) 101, 1160
BisI GCNGC 7 cut(s) 93, 105, 120, 702, 1290, 1383, 1386
BlsI GCNGC 7 cut(s) 94, 106, 121, 703, 1291, 1384, 1387
Bme1390I CCNGG 4 cut(s) 41, 535, 907, 933
Bme18I GGWCC 2 cut(s) 37, 368
BmgT120I GGNCC 3 cut(s) 37, 368, 968
BmiI GGNNCC 5 cut(s) 118, 970, 1145, 1263, 1433
BmrFI CCNGG 4 cut(s) 41, 535, 907, 933
BmsI GCATC 4 cut(s) 24, 429, 529, 1172
BpiI GAAGAC 2 cut(s) 142, 1301
Bpu10I CCTNAGC 1 cut(s) 1214
BpuEI CTTGAG 2 cut(s) 188, 1178
BpuMI CCSGG 1 cut(s) 41
BsaJI CCNNGG 5 cut(s) 534, 905, 931, 964, 972
BsaWI WCCGGW 1 cut(s) 756
BsaXI ACNNNNNCTCC 2 cut(s) 1059, 1089
Bsc4I CCNNNNNNNGG 3 cut(s) 227, 1201, 1366
Bse1I ACTGG 2 cut(s) 1098, 1409
Bse3DI GCAATG 2 cut(s) 1063, 1225
BseBI CCWGG 3 cut(s) 535, 907, 933
BseDI CCNNGG 5 cut(s) 534, 905, 931, 964, 972
BseGI GGATG 6 cut(s) 219, 370, 544, 644, 1084, 1409
BseLI CCNNNNNNNGG 3 cut(s) 227, 1201, 1366
BseMI GCAATG 2 cut(s) 1063, 1225
BseMII CTCAG 4 cut(s) 306, 318, 1362, 1438
BseNI ACTGG 2 cut(s) 1098, 1409
BseRI GAGGAG 1 cut(s) 260
BseXI GCAGC 5 cut(s) 79, 91, 1276, 1369, 1397
BsgI GTGCAG 1 cut(s) 1103
Bsh1236I CGCG 1 cut(s) 704
BshFI GGCC 2 cut(s) 969, 1122
BshNI GGYRCC 1 cut(s) 1143
BsiSI CCGG 2 cut(s) 40, 757
BslFI GGGAC 3 cut(s) 56, 913, 1355
BslI CCNNNNNNNGG 3 cut(s) 227, 1201, 1366
BsmAI GTCTC 4 cut(s) 63, 194, 480, 674
BsmFI GGGAC 3 cut(s) 56, 913, 1355
BsnI GGCC 2 cut(s) 969, 1122
Bsp1407I TGTACA 3 cut(s) 339, 956, 1417
Bsp143I GATC 4 cut(s) 433, 559, 909, 1030
Bsp19I CCATGG 1 cut(s) 972
BspACI CCGC 4 cut(s) 120, 429, 702, 1141
BspANI GGCC 2 cut(s) 969, 1122
BspCNI CTCAG 4 cut(s) 307, 319, 1363, 1437
BspFNI CGCG 1 cut(s) 704
BspLI GGNNCC 5 cut(s) 118, 970, 1145, 1263, 1433
BspMAI CTGCAG 1 cut(s) 1387
BspPI GGATC 3 cut(s) 428, 554, 917
BspT107I GGYRCC 1 cut(s) 1143
BsrDI GCAATG 2 cut(s) 1063, 1225
BsrGI TGTACA 3 cut(s) 339, 956, 1417
BsrI ACTGG 2 cut(s) 1098, 1409
BssECI CCNNGG 5 cut(s) 534, 905, 931, 964, 972
BssMI GATC 4 cut(s) 433, 559, 909, 1030
BssT1I CCWWGG 2 cut(s) 964, 972
Bst2UI CCWGG 3 cut(s) 535, 907, 933
Bst4CI ACNGT 4 cut(s) 298, 335, 400, 1307
Bst6I CTCTTC 2 cut(s) 197, 801
BstAPI GCANNNNNTGC 1 cut(s) 92
BstAUI TGTACA 3 cut(s) 339, 956, 1417
BstC8I GCNNGC 1 cut(s) 838
BstDEI CTNAG 5 cut(s) 315, 327, 1214, 1371, 1424
BstDSI CCRYGG 1 cut(s) 972
BstF5I GGATG 6 cut(s) 219, 370, 544, 644, 1084, 1409
BstFNI CGCG 1 cut(s) 704
BstKTI GATC 4 cut(s) 436, 562, 912, 1033
BstMAI GTCTC 4 cut(s) 63, 194, 480, 674
BstMBI GATC 4 cut(s) 433, 559, 909, 1030
BstMWI GCNNNNNNNGC 9 cut(s) 92, 101, 316, 426, 842, 1160, 1261, 1295, 1391
BstNI CCWGG 3 cut(s) 535, 907, 933
BstNSI RCATGY 1 cut(s) 1286
BstSCI CCNGG 4 cut(s) 39, 533, 905, 931
BstSFI CTRYAG 2 cut(s) 1293, 1383
BstUI CGCG 1 cut(s) 704
BstV1I GCAGC 5 cut(s) 79, 91, 1276, 1369, 1397
BstV2I GAAGAC 2 cut(s) 142, 1301
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
BsuRI GGCC 2 cut(s) 969, 1122
BtgI CCRYGG 1 cut(s) 972
BtgZI GCGATG 1 cut(s) 1169
BtsCI GGATG 6 cut(s) 219, 370, 544, 644, 1084, 1409
BtsIMutI CAGTG 2 cut(s) 294, 396
Cac8I GCNNGC 1 cut(s) 838
Cfr13I GGNCC 3 cut(s) 37, 368, 968
CseI GACGC 1 cut(s) 693
Csp6I GTAC 6 cut(s) 340, 869, 957, 1091, 1144, 1418
CviQI GTAC 6 cut(s) 340, 869, 957, 1091, 1144, 1418
DdeI CTNAG 5 cut(s) 315, 327, 1214, 1371, 1424
DpnI GATC 4 cut(s) 435, 561, 911, 1032
DpnII GATC 4 cut(s) 433, 559, 909, 1030
Eam1104I CTCTTC 2 cut(s) 197, 801
EarI CTCTTC 2 cut(s) 197, 801
Eco130I CCWWGG 2 cut(s) 964, 972
Eco32I GATATC 1 cut(s) 1239
Eco47I GGWCC 2 cut(s) 37, 368
Eco57I CTGAAG 4 cut(s) 178, 585, 690, 903
EcoRI GAATTC 1 cut(s) 511
EcoRII CCWGG 3 cut(s) 533, 905, 931
EcoRV GATATC 1 cut(s) 1239
EcoT14I CCWWGG 2 cut(s) 964, 972
EcoT22I ATGCAT 1 cut(s) 544
ErhI CCWWGG 2 cut(s) 964, 972
FalI AAGNNNNNCTT 2 cut(s) 1140, 1172
FaqI GGGAC 3 cut(s) 56, 913, 1355
FauNDI CATATG 1 cut(s) 772
Fnu4HI GCNGC 7 cut(s) 93, 105, 120, 702, 1290, 1383, 1386
FokI GGATG 6 cut(s) 226, 377, 551, 651, 1091, 1416
Fsp4HI GCNGC 7 cut(s) 93, 105, 120, 702, 1290, 1383, 1386
FspBI CTAG 3 cut(s) 135, 381, 723
GluI GCNGC 7 cut(s) 93, 105, 120, 702, 1290, 1383, 1386
HaeIII GGCC 2 cut(s) 969, 1122
HapII CCGG 2 cut(s) 40, 757
HgaI GACGC 1 cut(s) 693
HinfI GANTC 4 cut(s) 198, 233, 686, 943
HpaII CCGG 2 cut(s) 40, 757
Hpy166II GTNNAC 1 cut(s) 712
Hpy188I TCNGA 7 cut(s) 197, 230, 316, 598, 883, 1243, 1275
Hpy188III TCNNGA 5 cut(s) 167, 563, 762, 1334, 1426
Hpy8I GTNNAC 1 cut(s) 712
HpyAV CCTTC 4 cut(s) 560, 571, 593, 629
HpyCH4III ACNGT 4 cut(s) 298, 335, 400, 1307
HpyCH4IV ACGT 4 cut(s) 441, 939, 948, 1346
HpyCH4V TGCA 8 cut(s) 388, 420, 542, 1049, 1056, 1084, 1255, 1385
HpyF10VI GCNNNNNNNGC 9 cut(s) 92, 101, 316, 426, 842, 1160, 1261, 1295, 1391
HpyF3I CTNAG 5 cut(s) 315, 327, 1214, 1371, 1424
HpySE526I ACGT 4 cut(s) 441, 939, 948, 1346
KpnI GGTACC 1 cut(s) 1147
Kzo9I GATC 4 cut(s) 433, 559, 909, 1030
LmnI GCTCC 3 cut(s) 116, 273, 1261
Lsp1109I GCAGC 5 cut(s) 79, 91, 1276, 1369, 1397
LweI GCATC 4 cut(s) 24, 429, 529, 1172
MaeI CTAG 3 cut(s) 135, 381, 723
MaeII ACGT 4 cut(s) 441, 939, 948, 1346
MaeIII GTNAC 3 cut(s) 457, 944, 1355
MalI GATC 4 cut(s) 435, 561, 911, 1032
MboI GATC 4 cut(s) 433, 559, 909, 1030
MboII GAAGA 8 cut(s) 117, 142, 184, 743, 818, 896, 1025, 1301
MflI RGATCY 1 cut(s) 433
MluCI AATT 8 cut(s) 178, 413, 468, 478, 511, 617, 824, 849
MlyI GAGTC 1 cut(s) 952
MmeI TCCRAC 1 cut(s) 899
Mph1103I ATGCAT 1 cut(s) 544
MseI TTAA 5 cut(s) 498, 585, 848, 1071, 1302
MslI CAYNNNNRTG 1 cut(s) 1256
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 2 cut(s) 40, 757
MspR9I CCNGG 4 cut(s) 41, 535, 907, 933
MvaI CCWGG 3 cut(s) 535, 907, 933
MvnI CGCG 1 cut(s) 704
MwoI GCNNNNNNNGC 9 cut(s) 92, 101, 316, 426, 842, 1160, 1261, 1295, 1391
NciI CCSGG 1 cut(s) 41
NcoI CCATGG 1 cut(s) 972
NdeI CATATG 1 cut(s) 772
NdeII GATC 4 cut(s) 433, 559, 909, 1030
NlaIV GGNNCC 5 cut(s) 118, 970, 1145, 1263, 1433
NmuCI GTSAC 2 cut(s) 944, 1355
NsiI ATGCAT 1 cut(s) 544
NspI RCATGY 1 cut(s) 1286
PciI ACATGT 1 cut(s) 1282
PcsI WCGNNNNNNNCGW 1 cut(s) 945
PfeI GAWTC 3 cut(s) 198, 233, 686
PfoI TCCNGGA 1 cut(s) 39
PkrI GCNGC 7 cut(s) 94, 106, 121, 703, 1291, 1384, 1387
PleI GAGTC 1 cut(s) 951
PpsI GAGTC 1 cut(s) 951
PscI ACATGT 1 cut(s) 1282
PsiI TTATAA 1 cut(s) 1172
Psp6I CCWGG 3 cut(s) 533, 905, 931
PspGI CCWGG 3 cut(s) 533, 905, 931
PspN4I GGNNCC 5 cut(s) 118, 970, 1145, 1263, 1433
PspPI GGNCC 3 cut(s) 37, 368, 968
PsrI GAACNNNNNNTAC 2 cut(s) 323, 355
PstI CTGCAG 1 cut(s) 1387
PsuI RGATCY 1 cut(s) 433
PvuII CAGCTG 1 cut(s) 92
RsaI GTAC 6 cut(s) 341, 870, 958, 1092, 1145, 1419
RsaNI GTAC 6 cut(s) 340, 869, 957, 1091, 1144, 1418
RseI CAYNNNNRTG 1 cut(s) 1256
SaqAI TTAA 5 cut(s) 498, 585, 848, 1071, 1302
SatI GCNGC 7 cut(s) 93, 105, 120, 702, 1290, 1383, 1386
Sau3AI GATC 4 cut(s) 433, 559, 909, 1030
Sau96I GGNCC 3 cut(s) 37, 368, 968
SchI GAGTC 1 cut(s) 952
ScrFI CCNGG 4 cut(s) 41, 535, 907, 933
SfaNI GCATC 4 cut(s) 24, 429, 529, 1172
SfcI CTRYAG 2 cut(s) 1293, 1383
SinI GGWCC 2 cut(s) 37, 368
SmiMI CAYNNNNRTG 1 cut(s) 1256
SmlI CTYRAG 2 cut(s) 167, 1157
SmoI CTYRAG 2 cut(s) 167, 1157
Sse9I AATT 8 cut(s) 178, 413, 468, 478, 511, 617, 824, 849
SsiI CCGC 4 cut(s) 120, 429, 702, 1141
SspI AATATT 1 cut(s) 1012
SspMI CTAG 3 cut(s) 135, 381, 723
StyD4I CCNGG 4 cut(s) 39, 533, 905, 931
StyI CCWWGG 2 cut(s) 964, 972
TaaI ACNGT 4 cut(s) 298, 335, 400, 1307
TaiI ACGT 4 cut(s) 444, 942, 951, 1349
TaqI TCGA 2 cut(s) 423, 804
TasI AATT 8 cut(s) 178, 413, 468, 478, 511, 617, 824, 849
TatI WGTACW 4 cut(s) 339, 956, 1090, 1417
TauI GCSGC 2 cut(s) 122, 704
TfiI GAWTC 3 cut(s) 198, 233, 686
Tru1I TTAA 5 cut(s) 498, 585, 848, 1071, 1302
Tru9I TTAA 5 cut(s) 498, 585, 848, 1071, 1302
TscAI CASTG 2 cut(s) 301, 403
TseFI GTSAC 2 cut(s) 944, 1355
TseI GCWGC 5 cut(s) 92, 104, 1289, 1382, 1385
Tsp45I GTSAC 2 cut(s) 944, 1355
TspDTI ATGAA 7 cut(s) 100, 491, 648, 678, 790, 1025, 1240
TspGWI ACGGA 1 cut(s) 773
TspRI CASTG 2 cut(s) 301, 403
VpaK11BI GGWCC 2 cut(s) 37, 368
XapI RAATTY 4 cut(s) 413, 478, 511, 617
XceI RCATGY 1 cut(s) 1286
XcmI CCANNNNNNNNNTGG 1 cut(s) 848
XspI CTAG 3 cut(s) 135, 381, 723
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.