Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000881

Physical Location & Seq

Genomic Coordinates Forward (+)

13059210 .. 13063218

Gene Structure 4009 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1161700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1689 bp

ATGCACGCTGGAACCAATCCATTTGAAGTTATCACCCAAGCTGTAAAGGCTGTGGAAAAACACATGAAAACTTTTGTTCATCGCGAGAAGAAAAAGTTGCCTTCTTTTCTGGACTGTTTTGGCTGGTGTACATGGGATGCCTTTTACACCGATGTCACAGCTGAGGGTGTGGATCAAGGCCTTAAAAGCTTATCGGAGGGAGGGACTCCTCCGCGGTTCCTAATCGTTGATGACGGTTGGCAGCAGATAGAAAATAAAGACAAGGATTCTGGTGTCGTTGTACAAGAAGGAGCACAGTTTGCAAGTAGGTTGACAGGAATCAAAGAGAACGAAAAGTTCCAAAAGAACGACCACAACAATGAGCAAGTCTCTGGCCTGAAACATGTTGTGGATGAAGCAAAGCAGCATCACAATGTGAAATTTGTGTATGTATGGCATGCTCTAGCTGGGTACTGGGGTGGAGTAAAACCAGCTGCTACTGGCATGGAACACTGTGACACTGCTTTGGCATACCCAGTGTCGTCCCCTGGTGTAGAGGGCAACCAACCAGACATAGTTATGGACAGCTTAACTGTTCATGGTCTTGGTCTGGTTCATCCAAAGAAAGTTTTCAATTTCTACAATGAGCTTCATTCCTACTTGGCTTCCTGTGGAGTTTATGGAGTTAAGGCATTTGAGGCTTTGGTTGCTCGAAATTTTGCTGACAACGGATGCATTTCTTGCATGTGTCACAACACTGATGGGCTCTACAGTGCCAAGCAGACTGCTGTGGTCAGAGCCTCTGATGACTTCTATCCCCGAGATCCTGCTTCACACACAATTCATATATCTTCCGTTGCTTATAACACCCTGTTCCTTGGAGAATTCATGCAACCTGACTGGGATATGTTTCACAGTTTACACCCAGCGGCAGAGTATCACGGTGCAGCTCGTGCTCTTGGTGGATGTGCAATCTATGTCAGTGATAAGCCAGGCCATCACAATTTTGATCTTCTGAGGAAGCTGGTTCTCCCTGATGGATCTGTCCTCCGTGCCAAGTTACCTGGCAGGCCTACCCGTGATTGTCTCTTCGCTGATCCAGCAAGAGACAGGACCAGCTTGCTCAAAATATGGAATGTCAACAATCTCTCTGGTGTGGTTGGTGTGTTTAACTGTCAAGGTGCCGTTTATGGTTCTGTTCGTGCCGCTGATGTTGATGTCATTGCTCAAGTTGCTGGTGCTGATTGGAATGGAGAAACAGTAGTTTATGCTCATAAGTCAGGTGAGGTTCTTCGGTTGCCAAAAGGTGCTTCAGTGCCTGTGACGCTTAAAGTTCTTGACTATGAGGTTTTCCATTTCTGTCCTCTCAAGGAAATTACATCCGACGTCTCATTTGCACCAATAGGCCTACTTGACATGTTCAATTCAAGTGGTGCTGTGGAGGAGGTTGAAATCCATTTGGCTTCTGACAAGAAACCAGAGCTTTCTAATGGAGAAGTTAGCGAGAACCGATCTCCAACTGCGACAATTGGTCTCAAAACCAGAGGATGTGGAAGGTTTGGAGCTTACTGTTCCCGGCGGCCGCTGAAGTGCTCTGTTGACAACCCTGAGACCAACTTCGAATATGACTCTGCATCTGGATTAACGACGATTACCATTCCAGTACCAGAAAAAGAGATGTACAGATGGTCAGTAGAGATCCAAGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005911 GO:0005975 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009506 GO:0009628 GO:0015925 GO:0016052 GO:0016787 GO:0016798 GO:0030054 GO:0033530 GO:0034484 GO:0044238 GO:0050896 GO:0052692 GO:0055044 GO:0071704 GO:0080167 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00052 map00052

Pfam Domains

Protein Families

Raffinose_syn

Protein Analysis

563

Amino Acids

61.74

Weight (kDa)

5.96

Isoelectric Point (pI)

29.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Raffinose_syn	PF05691	1 - 223	4.8e-102	Raffinose synthase or seed imbibition protein Sip1
Raffinose_syn	PF05691	223 - 545	1.2e-130	Raffinose synthase or seed imbibition protein Sip1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57520 AT3G57520 AT3G57520
fragaria_vesca	FvH4_3g00160 FvH4_6g23550 FvH4_6g23550 FvH4_6g23550 FvH4_6g23550
malus_domestica	MD09G1161700.v1.1 MD09G1162100.v1.1 MD10G1341400.v1.1 MD10G1341500.v1.1 MD10G1341600.v1.1 MD12G1077800.v1.1 MD14G1070300.v1.1
prunus_persica	Prupe.4G001700_v2.0.a1 Prupe.5G162400_v2.0.a1 Prupe.5G162400_v2.0.a1 Prupe.7G064000_v2.0.a1 Prupe.7G064000_v2.0.a1
pyrus_communis	pycom10g29090 pycom12g06210 pycom14g05800 pycom14g05880 pycom14g05890 pycom14g05910 pycom14g05930
rosa_chinensis	RchiOBHm_Chr3g0481621 RchiOBHm_Chr5g0000211 RchiOBHm_Chr7g0192011 RchiOBHm_Chr7g0192021
rosa_laevigata	RLG00000004409 RLG00000023385 RLG00000030850
rosa_multiflora	Rmu_co8093432.1_g000001 Rmu_co8314355.1_g000001 Rmu_sc0010409.1_g000002 Rmu_sc0021936.1_g000002 Rmu_ssc0000243.1_g000010 Rmu_ssc0000421.1_g000033
rosa_roxburghii	Rroxscaffold_1G00075890 Rroxscaffold_3G00263360 Rroxscaffold_6G00400310
rosa_rugosa	Rorug03G0192700 Rorug03G0192800 Rorug03G0192900 Rorug04G0382500 Rorug06G0512300 Rorug06G0512300
rosa_samantha	Rh3AG244100 Rh3BG279100 Rh3BG279300 Rh3CG274100 Rh3DG270900 Rh5AG001400 Rh5BG002800 Rh5CG001700 Rh5DG001600 Rh7AG122700 Rh7AG122800 Rh7BG124300 Rh7CG127000 Rh7DG125800
rosa_wichuraiana	Rw3G021800 Rw5G000190 Rw7G010450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	843
AatII	GACGTC	1 cut(s)	1368
AccB1I	GGYRCC	1 cut(s)	1160
AccII	CGCG	2 cut(s)	84, 214
AciI	CCGC	6 cut(s)	212, 214, 908, 1185, 1558, 1562
AclWI	GGATC	5 cut(s)	180, 797, 1027, 1070, 1672
AcoI	YGGCCR	1 cut(s)	1559
AcsI	RAATTY	3 cut(s)	419, 694, 863
AcuI	CTGAAG	2 cut(s)	1275, 1586
AcyI	GRCGYC	1 cut(s)	1365
AdeI	CACNNNGTG	1 cut(s)	415
AfaI	GTAC	5 cut(s)	130, 282, 452, 1644, 1661
AflIII	ACRYGT	2 cut(s)	382, 1395
AgsI	TTSAA	5 cut(s)	26, 613, 1402, 1407, 1430
AhdI	GACNNNNNGTC	1 cut(s)	1509
AjnI	CCWGG	3 cut(s)	526, 970, 1042
AjuI	GAANNNNNNNTTGG	2 cut(s)	1273, 1305
Alw21I	GWGCWC	3 cut(s)	295, 937, 1574
Alw26I	GTCTC	6 cut(s)	373, 1070, 1080, 1372, 1517, 1583
AlwI	GGATC	5 cut(s)	180, 797, 1027, 1070, 1672
AlwNI	CAGNNNCTG	1 cut(s)	1298
Ama87I	CYCGRG	1 cut(s)	798
AoxI	GGCC	6 cut(s)	178, 373, 973, 1049, 1384, 1559
ApeKI	GCWGC	4 cut(s)	241, 403, 473, 926
ApoI	RAATTY	3 cut(s)	419, 694, 863
ArsI	GACNNNNNNTTYG	2 cut(s)	1355, 1387
Asp700I	GAANNNNTTC	1 cut(s)	608
AspS9I	GGNCC	1 cut(s)	1092
AsuC2I	CCSGG	1 cut(s)	1555
AsuHPI	GGTGA	2 cut(s)	25, 1274
AsuII	TTCGAA	1 cut(s)	1599
AvaI	CYCGRG	1 cut(s)	798
AvaII	GGWCC	1 cut(s)	1092
BaeI	ACNNNNGTAYC	2 cut(s)	442, 475
BanI	GGYRCC	1 cut(s)	1160
BanII	GRGCYC	1 cut(s)	747
BauI	CACGAG	1 cut(s)	930
Bbv12I	GWGCWC	3 cut(s)	295, 937, 1574
BbvCI	CCTCAGC	1 cut(s)	162
BbvI	GCAGC	4 cut(s)	253, 415, 460, 938
BccI	CCATC	4 cut(s)	734, 984, 1010, 1659
BceAI	ACGGC	1 cut(s)	1148
BciT130I	CCWGG	3 cut(s)	528, 972, 1044
BcnI	CCSGG	1 cut(s)	1555
BcoDI	GTCTC	6 cut(s)	373, 1070, 1080, 1372, 1517, 1583
BfaI	CTAG	1 cut(s)	443
BfmI	CTRYAG	1 cut(s)	748
BisI	GCNGC	8 cut(s)	242, 404, 474, 909, 927, 1185, 1559, 1562
BlsI	GCNGC	8 cut(s)	243, 405, 475, 910, 928, 1186, 1560, 1563
Bme1390I	CCNGG	4 cut(s)	528, 972, 1044, 1555
Bme18I	GGWCC	1 cut(s)	1092
BmeRI	GACNNNNNGTC	1 cut(s)	1509
BmeT110I	CYCGRG	1 cut(s)	798
BmgT120I	GGNCC	1 cut(s)	1092
BmiI	GGNNCC	3 cut(s)	13, 218, 1162
BmrFI	CCNGG	4 cut(s)	528, 972, 1044, 1555
BmrI	ACTGGG	3 cut(s)	463, 509, 889
BmsI	GCATC	4 cut(s)	127, 415, 701, 1622
BmuI	ACTGGG	3 cut(s)	463, 509, 889
BplI	GAGNNNNNCTC	2 cut(s)	353, 385
Bpu10I	CCTNAGC	1 cut(s)	162
Bpu14I	TTCGAA	1 cut(s)	1599
BpuEI	CTTGAG	2 cut(s)	1191, 1331
BpuMI	CCSGG	1 cut(s)	1555
BsaHI	GRCGYC	1 cut(s)	1365
BsaI	GGTCTC	2 cut(s)	1517, 1583
BsaJI	CCNNGG	3 cut(s)	212, 526, 856
BsaXI	ACNNNNNCTCC	2 cut(s)	1224, 1254
Bse1I	ACTGG	5 cut(s)	458, 484, 515, 884, 1640
Bse3DI	GCAATG	1 cut(s)	1200
BseBI	CCWGG	3 cut(s)	528, 972, 1044
BseDI	CCNNGG	3 cut(s)	212, 526, 856
BseGI	GGATG	7 cut(s)	142, 397, 595, 716, 950, 1358, 1532
BseMI	GCAATG	1 cut(s)	1200
BseMII	CTCAG	3 cut(s)	153, 986, 1578
BseNI	ACTGG	5 cut(s)	458, 484, 515, 884, 1640
BseRI	GAGGAG	2 cut(s)	198, 1436
BseX3I	CGGCCG	1 cut(s)	1559
BseXI	GCAGC	4 cut(s)	253, 415, 460, 938
BseYI	CCCAGC	2 cut(s)	446, 904
BsgI	GTGCAG	1 cut(s)	945
Bsh1236I	CGCG	2 cut(s)	84, 214
Bsh1285I	CGRYCG	1 cut(s)	1562
BshFI	GGCC	6 cut(s)	180, 375, 975, 1051, 1386, 1561
BshNI	GGYRCC	1 cut(s)	1160
BsiEI	CGRYCG	1 cut(s)	1562
BsiHKAI	GWGCWC	3 cut(s)	295, 937, 1574
BsiHKCI	CYCGRG	1 cut(s)	798
BsiSI	CCGG	1 cut(s)	1555
BslFI	GGGAC	2 cut(s)	217, 508
BsmAI	GTCTC	6 cut(s)	373, 1070, 1080, 1372, 1517, 1583
BsmBI	CGTCTC	1 cut(s)	1372
BsmFI	GGGAC	2 cut(s)	217, 508
BsnI	GGCC	6 cut(s)	180, 375, 975, 1051, 1386, 1561
Bso31I	GGTCTC	2 cut(s)	1517, 1583
BsoBI	CYCGRG	1 cut(s)	798
Bsp119I	TTCGAA	1 cut(s)	1599
Bsp1286I	GDGCHC	4 cut(s)	295, 747, 937, 1574
Bsp1407I	TGTACA	3 cut(s)	128, 280, 1659
Bsp143I	GATC	7 cut(s)	172, 802, 988, 1019, 1075, 1490, 1677
Bsp68I	TCGCGA	1 cut(s)	84
BspACI	CCGC	6 cut(s)	212, 214, 908, 1185, 1558, 1562
BspANI	GGCC	6 cut(s)	180, 375, 975, 1051, 1386, 1561
BspCNI	CTCAG	3 cut(s)	154, 987, 1579
BspFNI	CGCG	2 cut(s)	84, 214
BspLI	GGNNCC	3 cut(s)	13, 218, 1162
BspPI	GGATC	5 cut(s)	180, 797, 1027, 1070, 1672
BspT104I	TTCGAA	1 cut(s)	1599
BspT107I	GGYRCC	1 cut(s)	1160
BspTNI	GGTCTC	2 cut(s)	1517, 1583
BsrDI	GCAATG	1 cut(s)	1200
BsrGI	TGTACA	3 cut(s)	128, 280, 1659
BsrI	ACTGG	5 cut(s)	458, 484, 515, 884, 1640
BssECI	CCNNGG	3 cut(s)	212, 526, 856
BssMI	GATC	7 cut(s)	172, 802, 988, 1019, 1075, 1490, 1677
BssNI	GRCGYC	1 cut(s)	1365
BssSI	CACGAG	1 cut(s)	930
BssT1I	CCWWGG	1 cut(s)	856
Bst2BI	CACGAG	1 cut(s)	930
Bst2UI	CCWGG	3 cut(s)	528, 972, 1044
Bst6I	CTCTTC	1 cut(s)	1073
BstACI	GRCGYC	1 cut(s)	1365
BstAPI	GCANNNNNTGC	3 cut(s)	299, 720, 932
BstAUI	TGTACA	3 cut(s)	128, 280, 1659
BstBI	TTCGAA	1 cut(s)	1599
BstC8I	GCNNGC	4 cut(s)	6, 438, 1049, 1100
BstDEI	CTNAG	3 cut(s)	162, 995, 1587
BstDSI	CCRYGG	1 cut(s)	212
BstF5I	GGATG	7 cut(s)	142, 397, 595, 716, 950, 1358, 1532
BstFNI	CGCG	2 cut(s)	84, 214
BstKTI	GATC	7 cut(s)	175, 805, 991, 1022, 1078, 1493, 1680
BstMAI	GTCTC	6 cut(s)	373, 1070, 1080, 1372, 1517, 1583
BstMBI	GATC	7 cut(s)	172, 802, 988, 1019, 1075, 1490, 1677
BstMCI	CGRYCG	1 cut(s)	1562
BstNI	CCWGG	3 cut(s)	528, 972, 1044
BstNSI	RCATGY	4 cut(s)	386, 440, 727, 1399
BstSCI	CCNGG	4 cut(s)	526, 970, 1042, 1553
BstSFI	CTRYAG	1 cut(s)	748
BstUI	CGCG	2 cut(s)	84, 214
BstV1I	GCAGC	4 cut(s)	253, 415, 460, 938
BstX2I	RGATCY	3 cut(s)	802, 1019, 1677
BstYI	RGATCY	3 cut(s)	802, 1019, 1677
BstZI	CGGCCG	1 cut(s)	1559
BsuRI	GGCC	6 cut(s)	180, 375, 975, 1051, 1386, 1561
BtgI	CCRYGG	1 cut(s)	212
BtgZI	GCGATG	1 cut(s)	65
BtsCI	GGATG	7 cut(s)	142, 397, 595, 716, 950, 1358, 1532
BtsI	GCAGTG	1 cut(s)	498
BtsIMutI	CAGTG	7 cut(s)	490, 498, 522, 735, 757, 967, 1299
BtuMI	TCGCGA	1 cut(s)	84
Cac8I	GCNNGC	4 cut(s)	6, 438, 1049, 1100
CaiI	CAGNNNCTG	1 cut(s)	1298
CciNI	GCGGCCGC	1 cut(s)	1559
Cfr13I	GGNCC	1 cut(s)	1092
Cfr42I	CCGCGG	1 cut(s)	215
CseI	GACGC	1 cut(s)	1312
Csp6I	GTAC	5 cut(s)	129, 281, 451, 1643, 1660
CspCI	CAANNNNNGTGG	2 cut(s)	1390, 1425
CviAII	CATG	9 cut(s)	64, 132, 383, 437, 484, 578, 724, 868, 1396
CviQI	GTAC	5 cut(s)	129, 281, 451, 1643, 1660
DdeI	CTNAG	3 cut(s)	162, 995, 1587
DpnI	GATC	7 cut(s)	174, 804, 990, 1021, 1077, 1492, 1679
DpnII	GATC	7 cut(s)	172, 802, 988, 1019, 1075, 1490, 1677
DraIII	CACNNNGTG	1 cut(s)	415
DriI	GACNNNNNGTC	1 cut(s)	1509
EaeI	YGGCCR	1 cut(s)	1559
EagI	CGGCCG	1 cut(s)	1559
Eam1104I	CTCTTC	1 cut(s)	1073
Eam1105I	GACNNNNNGTC	1 cut(s)	1509
EarI	CTCTTC	1 cut(s)	1073
EclXI	CGGCCG	1 cut(s)	1559
Eco130I	CCWWGG	1 cut(s)	856
Eco147I	AGGCCT	3 cut(s)	180, 1051, 1386
Eco24I	GRGCYC	1 cut(s)	747
Eco31I	GGTCTC	2 cut(s)	1517, 1583
Eco47I	GGWCC	1 cut(s)	1092
Eco52I	CGGCCG	1 cut(s)	1559
Eco57I	CTGAAG	2 cut(s)	1275, 1586
Eco88I	CYCGRG	1 cut(s)	798
EcoRI	GAATTC	1 cut(s)	863
EcoRII	CCWGG	3 cut(s)	526, 970, 1042
EcoT14I	CCWWGG	1 cut(s)	856
EcoT22I	ATGCAT	1 cut(s)	716
EcoT38I	GRGCYC	1 cut(s)	747
ErhI	CCWWGG	1 cut(s)	856
Esp3I	CGTCTC	1 cut(s)	1372
FaeI	CATG	9 cut(s)	67, 135, 386, 440, 487, 581, 727, 871, 1399
FaqI	GGGAC	2 cut(s)	217, 508
FatI	CATG	9 cut(s)	63, 131, 382, 436, 483, 577, 723, 867, 1395
Fnu4HI	GCNGC	8 cut(s)	242, 404, 474, 909, 927, 1185, 1559, 1562
FokI	GGATG	7 cut(s)	149, 404, 582, 723, 957, 1345, 1539
FriOI	GRGCYC	1 cut(s)	747
Fsp4HI	GCNGC	8 cut(s)	242, 404, 474, 909, 927, 1185, 1559, 1562
FspBI	CTAG	1 cut(s)	443
GluI	GCNGC	8 cut(s)	242, 404, 474, 909, 927, 1185, 1559, 1562
GsaI	CCCAGC	2 cut(s)	450, 908
HaeIII	GGCC	6 cut(s)	180, 375, 975, 1051, 1386, 1561
HapII	CCGG	1 cut(s)	1555
HgaI	GACGC	1 cut(s)	1312
Hin1I	GRCGYC	1 cut(s)	1365
Hin1II	CATG	9 cut(s)	67, 135, 386, 440, 487, 581, 727, 871, 1399
HincII	GTYRAC	3 cut(s)	312, 1120, 1579
HindII	GTYRAC	3 cut(s)	312, 1120, 1579
HindIII	AAGCTT	1 cut(s)	187
HinfI	GANTC	4 cut(s)	205, 266, 318, 1607
HpaII	CCGG	1 cut(s)	1555
HphI	GGTGA	2 cut(s)	25, 1274
Hpy166II	GTNNAC	5 cut(s)	129, 312, 899, 1120, 1579
Hpy188I	TCNGA	6 cut(s)	196, 776, 784, 996, 1363, 1447
Hpy188III	TCNNGA	4 cut(s)	83, 110, 1316, 1617
Hpy8I	GTNNAC	5 cut(s)	129, 312, 899, 1120, 1579
Hpy99I	CGWCG	2 cut(s)	1367, 1630
HpyAV	CCTTC	3 cut(s)	111, 281, 1527
HpyCH4IV	ACGT	1 cut(s)	1365
HpyCH4V	TGCA	9 cut(s)	4, 302, 714, 723, 871, 926, 950, 1376, 1613
HpyF3I	CTNAG	3 cut(s)	162, 995, 1587
HpySE526I	ACGT	1 cut(s)	1365
Hsp92I	GRCGYC	1 cut(s)	1365
Hsp92II	CATG	9 cut(s)	67, 135, 386, 440, 487, 581, 727, 871, 1399
KspI	CCGCGG	1 cut(s)	215
Kzo9I	GATC	7 cut(s)	172, 802, 988, 1019, 1075, 1490, 1677
LmnI	GCTCC	2 cut(s)	290, 1541
Lsp1109I	GCAGC	4 cut(s)	253, 415, 460, 938
LweI	GCATC	4 cut(s)	127, 415, 701, 1622
MaeI	CTAG	1 cut(s)	443
MaeII	ACGT	1 cut(s)	1365
MaeIII	GTNAC	5 cut(s)	154, 494, 728, 1038, 1300
MalI	GATC	7 cut(s)	174, 804, 990, 1021, 1077, 1492, 1679
MboI	GATC	7 cut(s)	172, 802, 988, 1019, 1075, 1490, 1677
MboII	GAAGA	5 cut(s)	100, 822, 983, 1060, 1262
MfeI	CAATTG	1 cut(s)	1506
MflI	RGATCY	3 cut(s)	802, 1019, 1677
MhlI	GDGCHC	4 cut(s)	295, 747, 937, 1574
MluCI	AATT	9 cut(s)	419, 613, 694, 819, 863, 982, 1353, 1402, 1506
MlyI	GAGTC	2 cut(s)	199, 1601
MmeI	TCCRAC	2 cut(s)	1386, 1520
Mph1103I	ATGCAT	1 cut(s)	716
MroXI	GAANNNNTTC	1 cut(s)	608
MseI	TTAA	6 cut(s)	183, 569, 666, 1149, 1308, 1622
MslI	CAYNNNNRTG	3 cut(s)	357, 411, 557
MspA1I	CMGCKG	6 cut(s)	161, 214, 473, 908, 1187, 1564
MspI	CCGG	1 cut(s)	1555
MspR9I	CCNGG	4 cut(s)	528, 972, 1044, 1555
MunI	CAATTG	1 cut(s)	1506
MvaI	CCWGG	3 cut(s)	528, 972, 1044
MvnI	CGCG	2 cut(s)	84, 214
NciI	CCSGG	1 cut(s)	1555
NdeII	GATC	7 cut(s)	172, 802, 988, 1019, 1075, 1490, 1677
NlaIII	CATG	9 cut(s)	67, 135, 386, 440, 487, 581, 727, 871, 1399
NlaIV	GGNNCC	3 cut(s)	13, 218, 1162
NmuCI	GTSAC	4 cut(s)	154, 494, 728, 1300
NotI	GCGGCCGC	1 cut(s)	1559
NruI	TCGCGA	1 cut(s)	84
NsiI	ATGCAT	1 cut(s)	716
NspI	RCATGY	4 cut(s)	386, 440, 727, 1399
NspV	TTCGAA	1 cut(s)	1599
PaeI	GCATGC	1 cut(s)	440
PceI	AGGCCT	3 cut(s)	180, 1051, 1386
PciI	ACATGT	2 cut(s)	382, 1395
PdmI	GAANNNNTTC	1 cut(s)	608
PfeI	GAWTC	2 cut(s)	266, 318
PkrI	GCNGC	8 cut(s)	243, 405, 475, 910, 928, 1186, 1560, 1563
PleI	GAGTC	2 cut(s)	199, 1601
PpsI	GAGTC	2 cut(s)	199, 1601
PscI	ACATGT	2 cut(s)	382, 1395
PsiI	TTATAA	1 cut(s)	843
Psp6I	CCWGG	3 cut(s)	526, 970, 1042
PspFI	CCCAGC	2 cut(s)	446, 904
PspGI	CCWGG	3 cut(s)	526, 970, 1042
PspN4I	GGNNCC	3 cut(s)	13, 218, 1162
PspPI	GGNCC	1 cut(s)	1092
PstNI	CAGNNNCTG	1 cut(s)	1298
PsuI	RGATCY	3 cut(s)	802, 1019, 1677
PvuII	CAGCTG	2 cut(s)	161, 473
RruI	TCGCGA	1 cut(s)	84
RsaI	GTAC	5 cut(s)	130, 282, 452, 1644, 1661
RsaNI	GTAC	5 cut(s)	129, 281, 451, 1643, 1660
RseI	CAYNNNNRTG	3 cut(s)	357, 411, 557
SacII	CCGCGG	1 cut(s)	215
SaqAI	TTAA	6 cut(s)	183, 569, 666, 1149, 1308, 1622
SatI	GCNGC	8 cut(s)	242, 404, 474, 909, 927, 1185, 1559, 1562
Sau3AI	GATC	7 cut(s)	172, 802, 988, 1019, 1075, 1490, 1677
Sau96I	GGNCC	1 cut(s)	1092
SchI	GAGTC	2 cut(s)	199, 1601
ScrFI	CCNGG	4 cut(s)	528, 972, 1044, 1555
SduI	GDGCHC	4 cut(s)	295, 747, 937, 1574
SfaNI	GCATC	4 cut(s)	127, 415, 701, 1622
SfcI	CTRYAG	1 cut(s)	748
Sfr303I	CCGCGG	1 cut(s)	215
SfuI	TTCGAA	1 cut(s)	1599
SgrBI	CCGCGG	1 cut(s)	215
SinI	GGWCC	1 cut(s)	1092
SmiMI	CAYNNNNRTG	3 cut(s)	357, 411, 557
SmlI	CTYRAG	2 cut(s)	1206, 1346
SmoI	CTYRAG	2 cut(s)	1206, 1346
SphI	GCATGC	1 cut(s)	440
Sse9I	AATT	9 cut(s)	419, 613, 694, 819, 863, 982, 1353, 1402, 1506
SseBI	AGGCCT	3 cut(s)	180, 1051, 1386
SsiI	CCGC	6 cut(s)	212, 214, 908, 1185, 1558, 1562
SspMI	CTAG	1 cut(s)	443
StuI	AGGCCT	3 cut(s)	180, 1051, 1386
StyD4I	CCNGG	4 cut(s)	526, 970, 1042, 1553
StyI	CCWWGG	1 cut(s)	856
TaiI	ACGT	1 cut(s)	1368
TaqI	TCGA	2 cut(s)	691, 1599
TasI	AATT	9 cut(s)	419, 613, 694, 819, 863, 982, 1353, 1402, 1506
TatI	WGTACW	3 cut(s)	128, 280, 1659
TauI	GCSGC	4 cut(s)	911, 1187, 1561, 1564
TfiI	GAWTC	2 cut(s)	266, 318
Tru1I	TTAA	6 cut(s)	183, 569, 666, 1149, 1308, 1622
Tru9I	TTAA	6 cut(s)	183, 569, 666, 1149, 1308, 1622
TscAI	CASTG	7 cut(s)	497, 505, 522, 742, 757, 967, 1299
TseFI	GTSAC	4 cut(s)	154, 494, 728, 1300
TseI	GCWGC	4 cut(s)	241, 403, 473, 926
Tsp45I	GTSAC	4 cut(s)	154, 494, 728, 1300
TspDTI	ATGAA	8 cut(s)	68, 80, 408, 566, 584, 620, 812, 856
TspGWI	ACGGA	3 cut(s)	723, 823, 1019
TspRI	CASTG	7 cut(s)	497, 505, 522, 742, 757, 967, 1299
VpaK11BI	GGWCC	1 cut(s)	1092
XapI	RAATTY	3 cut(s)	419, 694, 863
XceI	RCATGY	4 cut(s)	386, 440, 727, 1399
XmnI	GAANNNNTTC	1 cut(s)	608
XspI	CTAG	1 cut(s)	443
ZraI	GACGTC	1 cut(s)	1366
Zsp2I	ATGCAT	1 cut(s)	716

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.