pycom10g29090

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000881
Physical Location & Seq
Forward (+)
29495256 .. 29501206
5951 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g29090.11

Sequence Viewer

Length: 2385 bp
ATGCTCCAGACCACCGTCTTTTTTCCGTCTTCCAGAGCCCTTCCACTCAACATCAACAACAGGATCATCTTCTCACGCGGACGGGGATTTGGATGGGGACGGGGGTCTGTGCGTCCTAACAGCAGCACTAGTGGAAGTAGTAACACCGCATCCGCATCCCCATGGAGCATAAAACCTGTCTTTGAAGATGGTATTCTCAGTGTCAATGGTAACGATGTGTTGACACACGTGCCTGACAATGTGGTCGTCACACCATTGACCGACTCATCCGCATTCGTTGGTGCCACTTCTCAAACTGCTTCTTCACGCCATGTTTTTAAGCTTGGACTCGTACGGGATGTCAGATTACTAAGTCTTTTTAGATTTAAACTTTGGTGGATGATACCAAGAGTGGGCAATACAGGAAGTGACATCCCAGTTGAAACTCAGATGTTGCTCCTGCAAGCAAAGTCAGCCGAAGAAGCTACTCCTCCTAATTATATCTTATTCTTGCCAGTTCTAGATGGTGAATTTAGAAGCAGCTTGCAGGGGAATTCATCGAATGAACTTGAGTTTTGTGTCGAAAGTGGTGACCCAGCTATCGTTACCTCCCAATCCCCAAAAGCAGTTTTTGTGAATTGTGGGAACCATCCTTTCGATCTGATGAAGGAATCTATGAAGATTTTGGAGAAGCATTTTGGAACTTTCTCTCTCAGAGAATCCAAACAGATGCCTGGAATGCTAGATTGCTTTGGCTGGTGTACCTGGGATGCCTTTTATCAAGGGGTTAATCCTCAAGGAATCAGAGATGGCCTGAAAAGTTTATCAGAGGGAGGTACTCCAGCGAAGTTTTTGATAATTGACGATGGATGGCAAGATACATCAAATGAGTTCCAAATAGAAGGGGAGCCATTTGTTGACGGATCACAGTTTGGTGGTAGATTAAATAGCATTCAAGAAAATAACAAGTTTCGTAGGACTACTAATAAAGAGGGTGAAAGTGAGACGCCAAGTAGTCTCAAGGATTTTGTATCTGAAATCAAGGGGACTTTTGGTCTTAAGTACGTCTATGTGTGGCATGCCCTGTTGGGATACTGGGGAGGGCTTCTTCCAAATGCTCTTGGAACTAAAAAGTACAATCCTGAGCTAAGATACCCAGTGCAGTCACCTGGGAATTTGGCAAATATGAGGGACTTAGCTATGGATTGCATGGAGAAATATGGTGTTGGTACAATTGATCCTGCAAAGGTGTATCAGTTTTATGATGATCTACACGGTTATCTTGTTTCACAAAACGTGGATGGGGTTAAGGTTGATGTTCAGAACATACTGGAGACGATATCAGCTGATTTAGGAGGGCGAGTCTCTCTGACCAGACAGTTCCAACAGGCACTGGAGAAGTCCATAGCCACCCACTTCCAAGACAACAGCATCATCTGCTGCATGGGACAAAGCACGGACTCCATTTACCATTCAAAAAGAAGTGCTAGTACTCGAGCATCTGACGATTACTACCCGAAAAACCCAACAACACAGACACTGCACATAGCTGCTGTGGCTTTCAACAGCATATTCCTTGGTGAAGTTGTTCTCCCAGATTGGGACATGTTCTATAGCCGTCATGAAGCAGCTGAGTTCCATGCTGCTGCTAGAGCTGTGGGAGGATGCGGAGTCTATATTAGTGACAAACCCGGCCATCATGATTTCGAGATACTTAAAAGGCTTGTACTTCCTGATGGATCAATTCTGCGAGCTAGATTCCCCGGGAGGCCATCACGGGATTGTTTATTTTTTGACCCCGTTACAGATGGGAAGAGCCTTCTAAAGATATGGAATTTGAACAAATACAATGGAGTTTTAGGCATTTTCAATTGCCAAGGAGCAGGGAACTGGCCTTGTCTAGAACAAGCTGTTCCGGTAGAAGTTAGTGCTGAAGTGTCTGGGAAGGTGACCCCTGCAGATATTGAGTATTTCGACGAGGTTTCTGGCAAGCTGTGGACAGGAGACTGTGCGGTCTATTCCTTCGCAAAAGGGCGTCTGTCTCGGTTACCAAAGGACAAGTCATTTGACGTTGCATTAAGAGTTCTCCAATGCGATGTCTTTACTGTATCTCCTATTAAGGTTTACAAGCAAAACGTTGAATTTGCAGCGATTGGATTATTGAACATGTACAATTCCGGGGGAGCTGTTGAAGCAGTTGAGTGCTTTAGTCATAACTCTAGCAGTGAAATACACATTAAGGGAAGAGGAGGAGGTAGCTTTGGAGCTTACTCTAGTTTAAAGCCCAAAACTTGCTCAGTGAACTCCAAAGACGAAGAGGAATTTGAATTTAGAGAAGAAGTCGACAATCTTTTAAAAGTAACAGTTCCTGCCACAACTAGTTGGAAAATTGTTCTACGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

795

Amino Acids

87.73

Weight (kDa)

5.92

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 242, 1991
AccB1I GGYRCC 1 cut(s) 281
AccI GTMKAC 1 cut(s) 2322
AccII CGCG 1 cut(s) 78
AciI CCGC 6 cut(s) 78, 147, 153, 270, 1647, 1991
AclI AACGTT 1 cut(s) 2115
AclWI GGATC 4 cut(s) 71, 910, 1211, 1726
AcoI YGGCCR 1 cut(s) 1672
AcsI RAATTY 7 cut(s) 509, 532, 1153, 1813, 2120, 2300, 2306
AcuI CTGAAG 1 cut(s) 1932
AcvI CACGTG 1 cut(s) 229
AcyI GRCGYC 2 cut(s) 986, 2014
AfaI GTAC 9 cut(s) 333, 742, 817, 1043, 1115, 1210, 1471, 1707, 2150
AfiI CCNNNNNNNGG 2 cut(s) 392, 1579
AflII CTTAAG 1 cut(s) 1037
AflIII ACRYGT 3 cut(s) 226, 1584, 2145
AhdI GACNNNNNGTC 1 cut(s) 1032
AhlI ACTAGT 2 cut(s) 128, 2357
AjnI CCWGG 3 cut(s) 712, 743, 1147
Alw26I GTCTC 6 cut(s) 977, 1001, 1307, 1348, 1977, 2025
AlwI GGATC 4 cut(s) 71, 910, 1211, 1726
AlwNI CAGNNNCTG 3 cut(s) 1372, 1519, 2348
Ama87I CYCGRG 2 cut(s) 1473, 1742
AoxI GGCC 4 cut(s) 790, 1672, 1748, 1871
ApeKI GCWGC 8 cut(s) 123, 519, 1419, 1529, 1607, 1622, 1625, 2126
ApoI RAATTY 7 cut(s) 509, 532, 1153, 1813, 2120, 2300, 2306
ArsI GACNNNNNNTTYG 4 cut(s) 2024, 2027, 2056, 2059
Asp700I GAANNNNTTC 1 cut(s) 1566
AsuC2I CCSGG 4 cut(s) 1671, 1743, 1744, 2158
AsuHPI GGTGA 6 cut(s) 518, 581, 986, 1137, 1571, 1939
AvaI CYCGRG 2 cut(s) 1473, 1742
BanI GGYRCC 1 cut(s) 281
BanII GRGCYC 1 cut(s) 40
BarI GAAGNNNNNNTAC 2 cut(s) 1453, 1485
BbrPI CACGTG 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 21
BbvI GCAGC 8 cut(s) 135, 531, 1406, 1516, 1609, 1612, 1619, 2138
BceAI ACGGC 1 cut(s) 1581
BciT130I CCWGG 3 cut(s) 714, 745, 1149
BciVI GTATCC 1 cut(s) 1064
BcnI CCSGG 4 cut(s) 1671, 1743, 1744, 2158
BcoDI GTCTC 6 cut(s) 977, 1001, 1307, 1348, 1977, 2025
BcuI ACTAGT 2 cut(s) 128, 2357
BfmI CTRYAG 2 cut(s) 1591, 1935
BfrI CTTAAG 1 cut(s) 1037
BfuI GTATCC 1 cut(s) 1064
BisI GCNGC 8 cut(s) 124, 520, 1420, 1530, 1608, 1623, 1626, 2127
BlsI GCNGC 8 cut(s) 125, 521, 1421, 1531, 1609, 1624, 1627, 2128
BmcAI AGTACT 1 cut(s) 1471
Bme1390I CCNGG 7 cut(s) 714, 745, 1149, 1671, 1743, 1744, 2158
BmeRI GACNNNNNGTC 1 cut(s) 1032
BmeT110I CYCGRG 2 cut(s) 1473, 1742
BmiI GGNNCC 3 cut(s) 283, 626, 888
BmrFI CCNGG 7 cut(s) 714, 745, 1149, 1671, 1743, 1744, 2158
BmrI ACTGGG 3 cut(s) 410, 1084, 1130
BmsI GCATC 7 cut(s) 158, 164, 699, 739, 1419, 1487, 1634
BmuI ACTGGG 3 cut(s) 410, 1084, 1130
BoxI GACNNNNGTC 2 cut(s) 14, 103
BpiI GAAGAC 1 cut(s) 21
BpmI CTGGAG 3 cut(s) 804, 1331, 1394
Bpu10I CCTNAGC 1 cut(s) 1122
BpuEI CTTGAG 3 cut(s) 569, 759, 983
BpuMI CCSGG 4 cut(s) 1671, 1743, 1744, 2158
BsaAI YACGTR 1 cut(s) 229
BsaHI GRCGYC 2 cut(s) 986, 2014
BsaJI CCNNGG 8 cut(s) 161, 744, 1148, 1555, 1741, 1742, 1855, 2157
BsaWI WCCGGW 1 cut(s) 1894
BsaXI ACNNNNNCTCC 4 cut(s) 1326, 1356, 2074, 2104
Bsc4I CCNNNNNNNGG 2 cut(s) 392, 1579
Bse1I ACTGG 7 cut(s) 416, 494, 1079, 1136, 1314, 1377, 1874
BseBI CCWGG 3 cut(s) 714, 745, 1149
BseDI CCNNGG 8 cut(s) 161, 744, 1148, 1555, 1741, 1742, 1855, 2157
BseLI CCNNNNNNNGG 2 cut(s) 392, 1579
BseMII CTCAG 6 cut(s) 211, 440, 706, 1113, 1602, 2289
BseNI ACTGG 7 cut(s) 416, 494, 1079, 1136, 1314, 1377, 1874
BseRI GAGGAG 3 cut(s) 459, 2241, 2244
BseXI GCAGC 8 cut(s) 135, 531, 1406, 1516, 1609, 1612, 1619, 2138
BseYI CCCAGC 1 cut(s) 574
BsgI GTGCAG 2 cut(s) 1160, 1505
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 4 cut(s) 792, 1674, 1750, 1873
BshNI GGYRCC 1 cut(s) 281
BsiHKCI CYCGRG 2 cut(s) 1473, 1742
BsiSI CCGG 4 cut(s) 1671, 1743, 1895, 2157
BsiWI CGTACG 1 cut(s) 331
BslFI GGGAC 5 cut(s) 111, 1039, 1184, 1440, 1595
BslI CCNNNNNNNGG 2 cut(s) 392, 1579
BsmAI GTCTC 6 cut(s) 977, 1001, 1307, 1348, 1977, 2025
BsmBI CGTCTC 2 cut(s) 977, 1307
BsmFI GGGAC 5 cut(s) 111, 1039, 1184, 1440, 1595
BsmI GAATGC 3 cut(s) 272, 723, 930
BsnI GGCC 4 cut(s) 792, 1674, 1750, 1873
BsoBI CYCGRG 2 cut(s) 1473, 1742
Bsp1286I GDGCHC 1 cut(s) 40
Bsp1407I TGTACA 1 cut(s) 2148
Bsp143I GATC 6 cut(s) 63, 637, 902, 1216, 1246, 1718
Bsp19I CCATGG 1 cut(s) 161
BspACI CCGC 6 cut(s) 78, 147, 153, 270, 1647, 1991
BspANI GGCC 4 cut(s) 792, 1674, 1750, 1873
BspCNI CTCAG 6 cut(s) 210, 439, 705, 1114, 1603, 2288
BspFNI CGCG 1 cut(s) 78
BspHI TCATGA 2 cut(s) 1600, 1678
BspLI GGNNCC 3 cut(s) 283, 626, 888
BspMAI CTGCAG 1 cut(s) 1939
BspPI GGATC 4 cut(s) 71, 910, 1211, 1726
BspQI GCTCTTC 1 cut(s) 1787
BspT107I GGYRCC 1 cut(s) 281
BspTI CTTAAG 1 cut(s) 1037
BsrGI TGTACA 1 cut(s) 2148
BsrI ACTGG 7 cut(s) 416, 494, 1079, 1136, 1314, 1377, 1874
BssECI CCNNGG 8 cut(s) 161, 744, 1148, 1555, 1741, 1742, 1855, 2157
BssMI GATC 6 cut(s) 63, 637, 902, 1216, 1246, 1718
BssNI GRCGYC 2 cut(s) 986, 2014
BssT1I CCWWGG 3 cut(s) 161, 1555, 1855
Bst2UI CCWGG 3 cut(s) 714, 745, 1149
Bst4CI ACNGT 7 cut(s) 16, 909, 1256, 1359, 1988, 2086, 2344
Bst6I CTCTTC 3 cut(s) 1787, 2218, 2289
BstACI GRCGYC 2 cut(s) 986, 2014
BstAFI CTTAAG 1 cut(s) 1037
BstAPI GCANNNNNTGC 1 cut(s) 1416
BstAUI TGTACA 1 cut(s) 2148
BstBAI YACGTR 1 cut(s) 229
BstC8I GCNNGC 5 cut(s) 444, 524, 1059, 1731, 1970
BstDEI CTNAG 9 cut(s) 197, 350, 426, 692, 1122, 1127, 1174, 1611, 2275
BstDSI CCRYGG 1 cut(s) 161
BstEII GGTNACC 3 cut(s) 569, 1927, 2025
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 6 cut(s) 66, 640, 905, 1219, 1249, 1721
BstMAI GTCTC 6 cut(s) 977, 1001, 1307, 1348, 1977, 2025
BstMBI GATC 6 cut(s) 63, 637, 902, 1216, 1246, 1718
BstMWI GCNNNNNNNGC 7 cut(s) 452, 461, 718, 1416, 1535, 1631, 2171
BstNI CCWGG 3 cut(s) 714, 745, 1149
BstNSI RCATGY 3 cut(s) 1061, 1588, 2149
BstPAI GACNNNNGTC 2 cut(s) 14, 103
BstPI GGTNACC 3 cut(s) 569, 1927, 2025
BstSCI CCNGG 7 cut(s) 712, 743, 1147, 1669, 1741, 1742, 2156
BstSFI CTRYAG 2 cut(s) 1591, 1935
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 8 cut(s) 135, 531, 1406, 1516, 1609, 1612, 1619, 2138
BstV2I GAAGAC 1 cut(s) 21
BsuI GTATCC 1 cut(s) 1064
BsuRI GGCC 4 cut(s) 792, 1674, 1750, 1873
BtgI CCRYGG 1 cut(s) 161
BtgZI GCGATG 1 cut(s) 2088
BtsI GCAGTG 2 cut(s) 1517, 2209
BtsIMutI CAGTG 6 cut(s) 205, 1143, 1370, 1517, 2209, 2283
Cac8I GCNNGC 5 cut(s) 444, 524, 1059, 1731, 1970
CaiI CAGNNNCTG 3 cut(s) 1372, 1519, 2348
CciI TCATGA 2 cut(s) 1600, 1678
Cfr9I CCCGGG 1 cut(s) 1742
CseI GACGC 3 cut(s) 101, 994, 2003
Csp6I GTAC 9 cut(s) 332, 741, 816, 1042, 1114, 1209, 1470, 1706, 2149
CviQI GTAC 9 cut(s) 332, 741, 816, 1042, 1114, 1209, 1470, 1706, 2149
DdeI CTNAG 9 cut(s) 197, 350, 426, 692, 1122, 1127, 1174, 1611, 2275
DpnI GATC 6 cut(s) 65, 639, 904, 1218, 1248, 1720
DpnII GATC 6 cut(s) 63, 637, 902, 1216, 1246, 1718
DraI TTTAAA 3 cut(s) 367, 2259, 2334
DrdI GACNNNNNNGTC 2 cut(s) 242, 1991
DriI GACNNNNNGTC 1 cut(s) 1032
DseDI GACNNNNNNGTC 2 cut(s) 242, 1991
EaeI YGGCCR 1 cut(s) 1672
Eam1104I CTCTTC 3 cut(s) 1787, 2218, 2289
Eam1105I GACNNNNNGTC 1 cut(s) 1032
EarI CTCTTC 3 cut(s) 1787, 2218, 2289
Eco130I CCWWGG 3 cut(s) 161, 1555, 1855
Eco24I GRGCYC 1 cut(s) 40
Eco32I GATATC 1 cut(s) 1320
Eco57I CTGAAG 1 cut(s) 1932
Eco72I CACGTG 1 cut(s) 229
Eco88I CYCGRG 2 cut(s) 1473, 1742
Eco91I GGTNACC 3 cut(s) 569, 1927, 2025
EcoO65I GGTNACC 3 cut(s) 569, 1927, 2025
EcoRI GAATTC 1 cut(s) 532
EcoRII CCWGG 3 cut(s) 712, 743, 1147
EcoRV GATATC 1 cut(s) 1320
EcoT14I CCWWGG 3 cut(s) 161, 1555, 1855
EcoT38I GRGCYC 1 cut(s) 40
ErhI CCWWGG 3 cut(s) 161, 1555, 1855
Esp3I CGTCTC 2 cut(s) 977, 1307
FaqI GGGAC 5 cut(s) 111, 1039, 1184, 1440, 1595
FblI GTMKAC 1 cut(s) 2322
Fnu4HI GCNGC 8 cut(s) 124, 520, 1420, 1530, 1608, 1623, 1626, 2127
FriOI GRGCYC 1 cut(s) 40
Fsp4HI GCNGC 8 cut(s) 124, 520, 1420, 1530, 1608, 1623, 1626, 2127
GluI GCNGC 8 cut(s) 124, 520, 1420, 1530, 1608, 1623, 1626, 2127
GsaI CCCAGC 1 cut(s) 578
GsuI CTGGAG 3 cut(s) 804, 1331, 1394
HaeIII GGCC 4 cut(s) 792, 1674, 1750, 1873
HapII CCGG 4 cut(s) 1671, 1743, 1895, 2157
HgaI GACGC 3 cut(s) 101, 994, 2003
Hin1I GRCGYC 2 cut(s) 986, 2014
HincII GTYRAC 3 cut(s) 222, 898, 2323
HindII GTYRAC 3 cut(s) 222, 898, 2323
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 9 cut(s) 263, 327, 650, 698, 780, 1341, 1439, 1650, 1737
HpaII CCGG 4 cut(s) 1671, 1743, 1895, 2157
HphI GGTGA 6 cut(s) 518, 581, 986, 1137, 1571, 1939
Hpy166II GTNNAC 7 cut(s) 222, 741, 898, 1977, 2104, 2281, 2323
Hpy8I GTNNAC 7 cut(s) 222, 741, 898, 1977, 2104, 2281, 2323
Hpy99I CGWCG 1 cut(s) 1958
HpyAV CCTTC 6 cut(s) 50, 640, 875, 1808, 1918, 2011
HpyCH4III ACNGT 7 cut(s) 16, 909, 1256, 1359, 1988, 2086, 2344
HpyCH4IV ACGT 6 cut(s) 228, 1044, 1275, 2049, 2115, 2377
HpyF10VI GCNNNNNNNGC 7 cut(s) 452, 461, 718, 1416, 1535, 1631, 2171
HpyF3I CTNAG 9 cut(s) 197, 350, 426, 692, 1122, 1127, 1174, 1611, 2275
HpySE526I ACGT 6 cut(s) 228, 1044, 1275, 2049, 2115, 2377
Hsp92I GRCGYC 2 cut(s) 986, 2014
Kzo9I GATC 6 cut(s) 63, 637, 902, 1216, 1246, 1718
LguI GCTCTTC 1 cut(s) 1787
LmnI GCTCC 7 cut(s) 9, 165, 441, 886, 1859, 2162, 2243
Lsp1109I GCAGC 8 cut(s) 135, 531, 1406, 1516, 1609, 1612, 1619, 2138
LweI GCATC 7 cut(s) 158, 164, 699, 739, 1419, 1487, 1634
MaeII ACGT 6 cut(s) 228, 1044, 1275, 2049, 2115, 2377
MalI GATC 6 cut(s) 65, 639, 904, 1218, 1248, 1720
MboI GATC 6 cut(s) 63, 637, 902, 1216, 1246, 1718
MfeI CAATTG 2 cut(s) 1212, 1849
MhlI GDGCHC 1 cut(s) 40
MlyI GAGTC 5 cut(s) 257, 321, 1350, 1433, 1659
MmeI TCCRAC 2 cut(s) 1387, 2342
MroXI GAANNNNTTC 1 cut(s) 1566
MslI CAYNNNNRTG 1 cut(s) 160
MspA1I CMGCKG 2 cut(s) 1325, 1610
MspCI CTTAAG 1 cut(s) 1037
MspI CCGG 4 cut(s) 1671, 1743, 1895, 2157
MspR9I CCNGG 7 cut(s) 714, 745, 1149, 1671, 1743, 1744, 2158
MunI CAATTG 2 cut(s) 1212, 1849
Mva1269I GAATGC 3 cut(s) 272, 723, 930
MvaI CCWGG 3 cut(s) 714, 745, 1149
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 7 cut(s) 452, 461, 718, 1416, 1535, 1631, 2171
NciI CCSGG 4 cut(s) 1671, 1743, 1744, 2158
NcoI CCATGG 1 cut(s) 161
NdeII GATC 6 cut(s) 63, 637, 902, 1216, 1246, 1718
NlaIV GGNNCC 3 cut(s) 283, 626, 888
NmuCI GTSAC 6 cut(s) 247, 407, 569, 1143, 1661, 1927
NspI RCATGY 3 cut(s) 1061, 1588, 2149
PaeI GCATGC 1 cut(s) 1061
PaeR7I CTCGAG 1 cut(s) 1473
PagI TCATGA 2 cut(s) 1600, 1678
PciI ACATGT 2 cut(s) 1584, 2145
PciSI GCTCTTC 1 cut(s) 1787
PctI GAATGC 3 cut(s) 272, 723, 930
PdmI GAANNNNTTC 1 cut(s) 1566
PfeI GAWTC 4 cut(s) 650, 698, 780, 1737
Pfl23II CGTACG 1 cut(s) 331
PkrI GCNGC 8 cut(s) 125, 521, 1421, 1531, 1609, 1624, 1627, 2128
PleI GAGTC 5 cut(s) 257, 321, 1349, 1433, 1658
PmaCI CACGTG 1 cut(s) 229
PmlI CACGTG 1 cut(s) 229
PpsI GAGTC 5 cut(s) 257, 321, 1349, 1433, 1658
Ppu21I YACGTR 1 cut(s) 229
PscI ACATGT 2 cut(s) 1584, 2145
PshAI GACNNNNGTC 2 cut(s) 14, 103
Psp1406I AACGTT 1 cut(s) 2115
Psp6I CCWGG 3 cut(s) 712, 743, 1147
PspCI CACGTG 1 cut(s) 229
PspEI GGTNACC 3 cut(s) 569, 1927, 2025
PspFI CCCAGC 1 cut(s) 574
PspGI CCWGG 3 cut(s) 712, 743, 1147
PspLI CGTACG 1 cut(s) 331
PspN4I GGNNCC 3 cut(s) 283, 626, 888
PspXI VCTCGAGB 1 cut(s) 1473
PstI CTGCAG 1 cut(s) 1939
PstNI CAGNNNCTG 3 cut(s) 1372, 1519, 2348
PvuII CAGCTG 2 cut(s) 1325, 1610
RsaI GTAC 9 cut(s) 333, 742, 817, 1043, 1115, 1210, 1471, 1707, 2150
RsaNI GTAC 9 cut(s) 332, 741, 816, 1042, 1114, 1209, 1470, 1706, 2149
RseI CAYNNNNRTG 1 cut(s) 160
SalI GTCGAC 1 cut(s) 2321
SapI GCTCTTC 1 cut(s) 1787
SatI GCNGC 8 cut(s) 124, 520, 1420, 1530, 1608, 1623, 1626, 2127
Sau3AI GATC 6 cut(s) 63, 637, 902, 1216, 1246, 1718
ScaI AGTACT 1 cut(s) 1471
SchI GAGTC 5 cut(s) 257, 321, 1350, 1433, 1659
ScrFI CCNGG 7 cut(s) 714, 745, 1149, 1671, 1743, 1744, 2158
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 7 cut(s) 158, 164, 699, 739, 1419, 1487, 1634
SfcI CTRYAG 2 cut(s) 1591, 1935
Sfr274I CTCGAG 1 cut(s) 1473
SlaI CTCGAG 1 cut(s) 1473
SmaI CCCGGG 1 cut(s) 1744
SmiMI CAYNNNNRTG 1 cut(s) 160
SmlI CTYRAG 5 cut(s) 548, 774, 998, 1037, 1473
SmoI CTYRAG 5 cut(s) 548, 774, 998, 1037, 1473
SpeI ACTAGT 2 cut(s) 128, 2357
SphI GCATGC 1 cut(s) 1061
SsiI CCGC 6 cut(s) 78, 147, 153, 270, 1647, 1991
StyD4I CCNGG 7 cut(s) 712, 743, 1147, 1669, 1741, 1742, 2156
StyI CCWWGG 3 cut(s) 161, 1555, 1855
TaaI ACNGT 7 cut(s) 16, 909, 1256, 1359, 1988, 2086, 2344
TaiI ACGT 6 cut(s) 231, 1047, 1278, 2052, 2118, 2380
TaqI TCGA 7 cut(s) 539, 561, 636, 1474, 1686, 1953, 2322
TaqII GACCGA 1 cut(s) 275
TatI WGTACW 4 cut(s) 1113, 1469, 1705, 2148
TfiI GAWTC 4 cut(s) 650, 698, 780, 1737
TscAI CASTG 6 cut(s) 205, 1143, 1377, 1524, 2209, 2283
TseFI GTSAC 6 cut(s) 247, 407, 569, 1143, 1661, 1927
TseI GCWGC 8 cut(s) 123, 519, 1419, 1529, 1607, 1622, 1625, 2126
Tsp45I GTSAC 6 cut(s) 247, 407, 569, 1143, 1661, 1927
TspDTI ATGAA 5 cut(s) 525, 558, 659, 671, 1617
TspGWI ACGGA 3 cut(s) 15, 915, 1451
TspMI CCCGGG 1 cut(s) 1742
TspRI CASTG 6 cut(s) 205, 1143, 1377, 1524, 2209, 2283
Vha464I CTTAAG 1 cut(s) 1037
XapI RAATTY 7 cut(s) 509, 532, 1153, 1813, 2120, 2300, 2306
XbaI TCTAGA 2 cut(s) 499, 1879
XceI RCATGY 3 cut(s) 1061, 1588, 2149
XhoI CTCGAG 1 cut(s) 1473
XmaI CCCGGG 1 cut(s) 1742
XmiI GTMKAC 1 cut(s) 2322
XmnI GAANNNNTTC 1 cut(s) 1566
ZrmI AGTACT 1 cut(s) 1471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.