RLG00000030850

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000881
Physical Location & Seq
Reverse (-)
195387 .. 200568
5182 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030850

Sequence Viewer

Length: 2376 bp
ATGCTTCACAGCACCGTCGTTTCCCCCAAGGCCCTTCAACTCAATCTCGGCTTCTCTTCATTTCTAGCCACAAACCAGAACCAGAGCAGGAGGAGGAGGAGGATCTTCTTTTGCTCACAAACCAGAGATGGTTCTGTGCGTGGTGGAGTTAAGACTAGCACTACTACTAGTAATGGATGGAGACAATCTATGTTTGTCGGTACGAAACCTACCCTTGAAGATTCCATTCTGAGTGCCAGTGGTAAACATGTGTTGACAGACGTGCCTCCCAATGTGGTCTTCACACCAATTCCCAACTCATCGGCAGCATTCCTTGGCGCCACTTCTCAAAATGCTACTTCACAATCACGCCATGTTTTTAAGCTCGGAGTCTTACGTGATGTCAGATTATTAAGTCTTTTTAGATTTAAACTTTGGTGGATGATACCACGAGTGGGCAGTACAGGAAGTGACATCCCTGTGGAAACTCAGCTGTTGCTCCTAGAAGCAAAGGAGGAGGAGGGAAAGGAAGATAGTACATCTTATATCTTATTCTTGCCAGTTCTGGATGGTGAATTTAGAAGCAGCTTGCAGGGAAATACATCCAATGAACTTGAGTTTTGCGTCGAAAGTGGTGACCCCGCTATAGTTGCCTCAGAATCCCTAAAAGCAGTTTTTGTGAATTGTGGGAACCATCCTTTCGATCTGGTGAACGAATCTATGAAGACTTTGGCGAAGCATTATGGAAGCTTTGCTCTTAGAGAAACGAAACAGATGCCTGGAATGCTGGATTGTTTTGGCTGGTGTACTTGGGATGCCTTTTATCAAGGAGTTAACCCTAAAGGAATTAGAGACGGCCTCAGAAGTTTATCAGAAGGAGGTACTCCAGCTAAGTTTTTGATAATCGATGATGGGTGGCAAGATACATCAAATGAGTTCCAAAAAGAAGGGGAGCCATTTGTTGAAGGGACACACACAACCAATATGGTTGATAGTGACAAACCAAGTGGTCTCAAGGATTTTGTTTCTGAGATCAAGAATTCCTTTGGCCTTAGGTATGTGTATGTATGGCATGCCCTTTTGGGATATTGGGGAGGGCTTGTTCCAAATGCTCCAGGAACTAAAAAGTATAACCCCAAGTTCAGACACCCAGTACAGTCACCTGGAAATTTGTCAAATATGAGGGATGGAGCTATGGATAGTATGGAGAAATATGGTGTTGGTGTAATAGATCCTGCAAAGGCATATCAATTTTATGATGATCTACACGGATATCTTGTTTCACAGGATGTAGATGGTGTTAAGGTTGATGTTCAGAACATACTGGAGACCATATCAGCAGGTTTAGGAGGGCGAGTCTCTCTAACTAGACGATTCCAACAGGCACTTGAGAAGTCCATAGCCACTCATTTCCAAGATAACAGCATAATCTGCTGCATGGGCCAAAGCACAGACTCCATTTACCATTCAAAAAGAAGTGCCATTACTCGAGCATCTGATGATTACTACCCAAAAAACCCAACAACACAGACACTGCACATTGCTGCAGTGGCTTTCAACAGCATATTCCTTGGTGAAGTTTTTGTCCCAGATTGGGACATGTTCTATAGCCGGCATGAGGCAGCTGAGTTCCATGCTGCTGCTAGAGCTGTGGGAGGTTGCGGAGTCTATGTAAGTGACAAACCTGGCCAGCATGATTTCGAGATACTTAAAAGGCTTGTTCTTCCTGATGGAACAGTTCTCAGAGCTAGATATCCTGGGAGGCCATCACGGGATTGTTTGTTTGTTGATCCAGTTATGGACGGGAAGAGTCTTCTGAAAATATGGAATTTGAACAAATGCAACGGAGTTATAGGCATTTTCAATTGCCAAGGAGCAGGAAGCTGGCCTTGTCTGGAACATATCGTCCAGGTAAAAGCTAGTGAGGAGCTATCTGGGAAGGTCTCTCCTGCTGATATCGAGTATTTTGAAGAGGTATCTGGAAAGCTTTGGACTGGAGATTGTGCAGTCTATTCCTTCAAACAAGGGTATCTGTCTCGTTTACCGAAGGACAAGTCATTTGATGTCACATTACAAATTCTCCAATGCGATGTGTTTACCGTATCCCCTATTAAGGTTTACAAGCAAAATATTCAATTTGCACCAATTGGATTATTGAACATGTACAATTCAGGTGGAGCTGTTGACAGTATTGACTCGTTCAGTGATGCCTTTAGTTGTGTTATACACATCAGGGGAAGAGGGGCTGGTAGCTTTGGAGCATACTCAAGTTCAAAGCCCAAATCTTGCTCGGTGAACTCGAAAGATGAGGGATTTGAATTCAGAGGTGATGACAATCTTTTGACTGTGACAATTCCTGCCGCAACTAGTTCTTGGAACATTAGTTTCTCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

792

Amino Acids

87.28

Weight (kDa)

6.4

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 73 - 777 5.9e-252 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1310
AccB1I GGYRCC 1 cut(s) 317
AciI CCGC 3 cut(s) 621, 1641, 2340
AclWI GGATC 3 cut(s) 110, 1205, 1763
AcoI YGGCCR 1 cut(s) 1666
AcsI RAATTY 6 cut(s) 554, 1018, 1147, 1807, 2055, 2297
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 7 cut(s) 202, 442, 517, 787, 862, 1134, 2144
AfiI CCNNNNNNNGG 4 cut(s) 434, 1573, 1597, 2092
AflIII ACRYGT 3 cut(s) 247, 1578, 2139
AhlI ACTAGT 2 cut(s) 167, 2345
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 6 cut(s) 757, 1093, 1141, 1663, 1735, 1887
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AloI GAACNNNNNNTCC 4 cut(s) 1065, 1097, 1869, 1901
Alw26I GTCTC 7 cut(s) 175, 825, 995, 1301, 1342, 1927, 2019
AlwI GGATC 3 cut(s) 110, 1205, 1763
AlwNI CAGNNNCTG 1 cut(s) 1513
Ama87I CYCGRG 1 cut(s) 1467
AoxI GGCC 7 cut(s) 30, 835, 1027, 1420, 1666, 1742, 1865
ApeKI GCWGC 7 cut(s) 305, 564, 1413, 1523, 1601, 1616, 1619
ApoI RAATTY 6 cut(s) 554, 1018, 1147, 1807, 2055, 2297
ArsI GACNNNNNNTTYG 4 cut(s) 2018, 2021, 2050, 2053
AspLEI GCGC 1 cut(s) 320
AspS9I GGNCC 2 cut(s) 31, 1420
AsuHPI GGTGA 7 cut(s) 563, 626, 700, 1131, 1565, 2284, 2318
AvaI CYCGRG 1 cut(s) 1467
AxyI CCTNAGG 1 cut(s) 1031
BaeI ACNNNNGTAYC 2 cut(s) 1991, 2024
BalI TGGCCA 1 cut(s) 1668
BanI GGYRCC 1 cut(s) 317
BarI GAAGNNNNNNTAC 2 cut(s) 1447, 1479
BauI CACGAG 1 cut(s) 429
BbsI GAAGAC 3 cut(s) 271, 710, 1784
BbvI GCAGC 7 cut(s) 317, 576, 1400, 1510, 1603, 1606, 1613
BccI CCATC 9 cut(s) 122, 171, 542, 681, 884, 1160, 1268, 1703, 1753
BceAI ACGGC 1 cut(s) 850
BcgI CGANNNNNNTGC 2 cut(s) 736, 770
BciT130I CCWGG 6 cut(s) 759, 1095, 1143, 1665, 1737, 1889
BciVI GTATCC 1 cut(s) 2092
BcoDI GTCTC 7 cut(s) 175, 825, 995, 1301, 1342, 1927, 2019
BcuI ACTAGT 2 cut(s) 167, 2345
BfaI CTAG 9 cut(s) 65, 156, 168, 482, 1347, 1623, 1728, 1899, 2346
BfmI CTRYAG 3 cut(s) 624, 1524, 1585
BfoI RGCGCY 1 cut(s) 321
BfuAI ACCTGC 1 cut(s) 1310
BfuI GTATCC 1 cut(s) 2092
BisI GCNGC 8 cut(s) 306, 565, 1414, 1524, 1602, 1617, 1620, 2340
BlsI GCNGC 8 cut(s) 307, 566, 1415, 1525, 1603, 1618, 1621, 2341
Bme1390I CCNGG 6 cut(s) 759, 1095, 1143, 1665, 1737, 1889
BmeT110I CYCGRG 1 cut(s) 1467
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 2 cut(s) 31, 1420
BmiI GGNNCC 3 cut(s) 319, 671, 933
BmrFI CCNGG 6 cut(s) 759, 1095, 1143, 1665, 1737, 1889
BmrI ACTGGG 1 cut(s) 1124
BmsI GCATC 4 cut(s) 744, 784, 1481, 2176
BmuI ACTGGG 1 cut(s) 1124
BpiI GAAGAC 3 cut(s) 271, 710, 1784
BplI GAGNNNNNCTC 2 cut(s) 822, 854
BpmI CTGGAG 4 cut(s) 849, 1077, 1325, 1995
BpuEI CTTGAG 4 cut(s) 614, 977, 1388, 2230
Bsa29I ATCGAT 1 cut(s) 885
BsaAI YACGTR 1 cut(s) 377
BsaBI GATNNNNATC 1 cut(s) 2313
BsaHI GRCGYC 1 cut(s) 318
BsaI GGTCTC 3 cut(s) 995, 1301, 1927
BsaJI CCNNGG 5 cut(s) 27, 313, 1549, 1736, 1849
BsaXI ACNNNNNCTCC 8 cut(s) 1065, 1095, 1320, 1350, 1635, 1665, 2043, 2073
Bsc4I CCNNNNNNNGG 4 cut(s) 434, 1573, 1597, 2092
Bse118I RCCGGY 1 cut(s) 1590
Bse1I ACTGG 6 cut(s) 237, 539, 1130, 1308, 1772, 1978
Bse21I CCTNAGG 1 cut(s) 1031
Bse3DI GCAATG 1 cut(s) 1518
Bse8I GATNNNNATC 1 cut(s) 2313
BseBI CCWGG 6 cut(s) 759, 1095, 1143, 1665, 1737, 1889
BseCI ATCGAT 1 cut(s) 885
BseDI CCNNGG 5 cut(s) 27, 313, 1549, 1736, 1849
BseGI GGATG 9 cut(s) 182, 426, 453, 553, 581, 673, 799, 1171, 1273
BseJI GATNNNNATC 1 cut(s) 2313
BseLI CCNNNNNNNGG 4 cut(s) 434, 1573, 1597, 2092
BseMI GCAATG 1 cut(s) 1518
BseMII CTCAG 7 cut(s) 221, 482, 648, 853, 999, 1596, 1735
BseNI ACTGG 6 cut(s) 237, 539, 1130, 1308, 1772, 1978
BseRI GAGGAG 6 cut(s) 106, 109, 112, 509, 512, 1919
BseXI GCAGC 7 cut(s) 317, 576, 1400, 1510, 1603, 1606, 1613
BsgI GTGCAG 2 cut(s) 1499, 2004
BshFI GGCC 7 cut(s) 32, 837, 1029, 1422, 1668, 1744, 1867
BshNI GGYRCC 1 cut(s) 317
BshVI ATCGAT 1 cut(s) 885
BsiHKCI CYCGRG 1 cut(s) 1467
BsiSI CCGG 1 cut(s) 1591
BslFI GGGAC 3 cut(s) 961, 1550, 1589
BslI CCNNNNNNNGG 4 cut(s) 434, 1573, 1597, 2092
BsmAI GTCTC 7 cut(s) 175, 825, 995, 1301, 1342, 1927, 2019
BsmBI CGTCTC 1 cut(s) 825
BsmFI GGGAC 3 cut(s) 961, 1550, 1589
BsmI GAATGC 2 cut(s) 308, 768
BsnI GGCC 7 cut(s) 32, 837, 1029, 1422, 1668, 1744, 1867
Bso31I GGTCTC 3 cut(s) 995, 1301, 1927
BsoBI CYCGRG 1 cut(s) 1467
Bsp1407I TGTACA 1 cut(s) 2142
Bsp143I GATC 6 cut(s) 102, 682, 1011, 1210, 1240, 1768
BspACI CCGC 3 cut(s) 621, 1641, 2340
BspANI GGCC 7 cut(s) 32, 837, 1029, 1422, 1668, 1744, 1867
BspCNI CTCAG 7 cut(s) 222, 481, 647, 852, 1000, 1597, 1734
BspDI ATCGAT 1 cut(s) 885
BspLI GGNNCC 3 cut(s) 319, 671, 933
BspMAI CTGCAG 1 cut(s) 1528
BspMI ACCTGC 1 cut(s) 1310
BspPI GGATC 3 cut(s) 110, 1205, 1763
BspT107I GGYRCC 1 cut(s) 317
BspTNI GGTCTC 3 cut(s) 995, 1301, 1927
BsrDI GCAATG 1 cut(s) 1518
BsrFI RCCGGY 1 cut(s) 1590
BsrGI TGTACA 1 cut(s) 2142
BsrI ACTGG 6 cut(s) 237, 539, 1130, 1308, 1772, 1978
BssAI RCCGGY 1 cut(s) 1590
BssECI CCNNGG 5 cut(s) 27, 313, 1549, 1736, 1849
BssMI GATC 6 cut(s) 102, 682, 1011, 1210, 1240, 1768
BssNI GRCGYC 1 cut(s) 318
BssSI CACGAG 1 cut(s) 429
BssT1I CCWWGG 4 cut(s) 27, 313, 1549, 1849
Bst2BI CACGAG 1 cut(s) 429
Bst2UI CCWGG 6 cut(s) 759, 1095, 1143, 1665, 1737, 1889
Bst4CI ACNGT 6 cut(s) 16, 1137, 1717, 2080, 2168, 2326
Bst6I CTCTTC 4 cut(s) 61, 1781, 1944, 2212
BstACI GRCGYC 1 cut(s) 318
BstAPI GCANNNNNTGC 1 cut(s) 1410
BstAUI TGTACA 1 cut(s) 2142
BstBAI YACGTR 1 cut(s) 377
BstC8I GCNNGC 5 cut(s) 569, 1053, 1592, 1670, 1865
BstEII GGTNACC 1 cut(s) 614
BstF5I GGATG 9 cut(s) 182, 426, 453, 553, 581, 673, 799, 1171, 1273
BstH2I RGCGCY 1 cut(s) 321
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 6 cut(s) 105, 685, 1014, 1213, 1243, 1771
BstMAI GTCTC 7 cut(s) 175, 825, 995, 1301, 1342, 1927, 2019
BstMBI GATC 6 cut(s) 102, 682, 1011, 1210, 1240, 1768
BstMWI GCNNNNNNNGC 6 cut(s) 629, 763, 1410, 1419, 1529, 1625
BstNI CCWGG 6 cut(s) 759, 1095, 1143, 1665, 1737, 1889
BstNSI RCATGY 4 cut(s) 251, 1055, 1582, 2143
BstPI GGTNACC 1 cut(s) 614
BstSCI CCNGG 6 cut(s) 757, 1093, 1141, 1663, 1735, 1887
BstSFI CTRYAG 3 cut(s) 624, 1524, 1585
BstV1I GCAGC 7 cut(s) 317, 576, 1400, 1510, 1603, 1606, 1613
BstV2I GAAGAC 3 cut(s) 271, 710, 1784
BstX2I RGATCY 2 cut(s) 102, 1210
BstYI RGATCY 2 cut(s) 102, 1210
Bsu15I ATCGAT 1 cut(s) 885
Bsu36I CCTNAGG 1 cut(s) 1031
BsuI GTATCC 1 cut(s) 2092
BsuRI GGCC 7 cut(s) 32, 837, 1029, 1422, 1668, 1744, 1867
BsuTUI ATCGAT 1 cut(s) 885
BtgZI GCGATG 1 cut(s) 2082
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 9 cut(s) 182, 426, 453, 553, 581, 673, 799, 1171, 1273
BtsI GCAGTG 2 cut(s) 1511, 1533
BtsIMutI CAGTG 4 cut(s) 244, 1511, 1533, 2188
BveI ACCTGC 1 cut(s) 1310
Cac8I GCNNGC 5 cut(s) 569, 1053, 1592, 1670, 1865
CaiI CAGNNNCTG 1 cut(s) 1513
CfoI GCGC 1 cut(s) 320
Cfr10I RCCGGY 1 cut(s) 1590
Cfr13I GGNCC 2 cut(s) 31, 1420
ClaI ATCGAT 1 cut(s) 885
CseI GACGC 1 cut(s) 592
Csp6I GTAC 7 cut(s) 201, 441, 516, 786, 861, 1133, 2143
CspCI CAANNNNNGTGG 4 cut(s) 967, 1002, 2134, 2169
CviAII CATG 9 cut(s) 248, 353, 1052, 1417, 1579, 1595, 1613, 1673, 2140
CviQI GTAC 7 cut(s) 201, 441, 516, 786, 861, 1133, 2143
DinI GGCGCC 1 cut(s) 319
DpnI GATC 6 cut(s) 104, 684, 1013, 1212, 1242, 1770
DpnII GATC 6 cut(s) 102, 682, 1011, 1210, 1240, 1768
DraI TTTAAA 1 cut(s) 409
EaeI YGGCCR 1 cut(s) 1666
Eam1104I CTCTTC 4 cut(s) 61, 1781, 1944, 2212
EarI CTCTTC 4 cut(s) 61, 1781, 1944, 2212
Eco130I CCWWGG 4 cut(s) 27, 313, 1549, 1849
Eco31I GGTCTC 3 cut(s) 995, 1301, 1927
Eco32I GATATC 3 cut(s) 1253, 1733, 1936
Eco81I CCTNAGG 1 cut(s) 1031
Eco88I CYCGRG 1 cut(s) 1467
Eco91I GGTNACC 1 cut(s) 614
EcoO109I RGGNCCY 1 cut(s) 31
EcoO65I GGTNACC 1 cut(s) 614
EcoRI GAATTC 2 cut(s) 1018, 2297
EcoRII CCWGG 6 cut(s) 757, 1093, 1141, 1663, 1735, 1887
EcoRV GATATC 3 cut(s) 1253, 1733, 1936
EcoT14I CCWWGG 4 cut(s) 27, 313, 1549, 1849
EgeI GGCGCC 1 cut(s) 319
EheI GGCGCC 1 cut(s) 319
ErhI CCWWGG 4 cut(s) 27, 313, 1549, 1849
Esp3I CGTCTC 1 cut(s) 825
FaeI CATG 9 cut(s) 251, 356, 1055, 1420, 1582, 1598, 1616, 1676, 2143
FalI AAGNNNNNCTT 4 cut(s) 1007, 1039, 1852, 1884
FaqI GGGAC 3 cut(s) 961, 1550, 1589
FatI CATG 9 cut(s) 247, 352, 1051, 1416, 1578, 1594, 1612, 1672, 2139
FauI CCCGC 1 cut(s) 628
Fnu4HI GCNGC 8 cut(s) 306, 565, 1414, 1524, 1602, 1617, 1620, 2340
FokI GGATG 9 cut(s) 189, 433, 440, 560, 568, 660, 806, 1178, 1280
Fsp4HI GCNGC 8 cut(s) 306, 565, 1414, 1524, 1602, 1617, 1620, 2340
FspBI CTAG 9 cut(s) 65, 156, 168, 482, 1347, 1623, 1728, 1899, 2346
GlaI GCGC 1 cut(s) 319
GluI GCNGC 8 cut(s) 306, 565, 1414, 1524, 1602, 1617, 1620, 2340
GsuI CTGGAG 4 cut(s) 849, 1077, 1325, 1995
HaeII RGCGCY 1 cut(s) 321
HaeIII GGCC 7 cut(s) 32, 837, 1029, 1422, 1668, 1744, 1867
HapII CCGG 1 cut(s) 1591
HgaI GACGC 1 cut(s) 592
HhaI GCGC 1 cut(s) 320
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 9 cut(s) 251, 356, 1055, 1420, 1582, 1598, 1616, 1676, 2143
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HincII GTYRAC 3 cut(s) 255, 814, 2164
HindII GTYRAC 3 cut(s) 255, 814, 2164
HindIII AAGCTT 2 cut(s) 727, 1964
HpaI GTTAAC 1 cut(s) 814
HpaII CCGG 1 cut(s) 1591
HphI GGTGA 7 cut(s) 563, 626, 700, 1131, 1565, 2284, 2318
Hpy188III TCNNGA 6 cut(s) 545, 1015, 1681, 1706, 1874, 1959
Hpy99I CGWCG 2 cut(s) 20, 608
HpyAV CCTTC 7 cut(s) 44, 848, 920, 938, 1912, 2005, 2020
HpyCH4III ACNGT 6 cut(s) 16, 1137, 1717, 2080, 2168, 2326
HpyCH4IV ACGT 2 cut(s) 261, 376
HpyCH4V TGCA 8 cut(s) 571, 1217, 1416, 1516, 1526, 1821, 1985, 2120
HpyF10VI GCNNNNNNNGC 6 cut(s) 629, 763, 1410, 1419, 1529, 1625
HpySE526I ACGT 2 cut(s) 261, 376
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 9 cut(s) 251, 356, 1055, 1420, 1582, 1598, 1616, 1676, 2143
HspAI GCGC 1 cut(s) 318
KasI GGCGCC 1 cut(s) 317
KroI GCCGGC 1 cut(s) 1590
KroNI GCCGGC 1 cut(s) 1592
KspAI GTTAAC 1 cut(s) 814
Kzo9I GATC 6 cut(s) 102, 682, 1011, 1210, 1240, 1768
LmnI GCTCC 8 cut(s) 483, 931, 1096, 1169, 1853, 1906, 2156, 2237
Lsp1109I GCAGC 7 cut(s) 317, 576, 1400, 1510, 1603, 1606, 1613
LweI GCATC 4 cut(s) 744, 784, 1481, 2176
MaeI CTAG 9 cut(s) 65, 156, 168, 482, 1347, 1623, 1728, 1899, 2346
MaeII ACGT 2 cut(s) 261, 376
MaeIII GTNAC 7 cut(s) 449, 614, 974, 1137, 1655, 2044, 2326
MalI GATC 6 cut(s) 104, 684, 1013, 1212, 1242, 1770
MboI GATC 6 cut(s) 102, 682, 1011, 1210, 1240, 1768
MfeI CAATTG 2 cut(s) 1843, 2124
MflI RGATCY 2 cut(s) 102, 1210
MlsI TGGCCA 1 cut(s) 1668
MluNI TGGCCA 1 cut(s) 1668
Mly113I GGCGCC 1 cut(s) 318
MlyI GAGTC 6 cut(s) 378, 1344, 1427, 1653, 1798, 2168
MmeI TCCRAC 1 cut(s) 1381
Mox20I TGGCCA 1 cut(s) 1668
MroNI GCCGGC 1 cut(s) 1590
MscI TGGCCA 1 cut(s) 1668
MseI TTAA 8 cut(s) 150, 360, 392, 408, 813, 1281, 1689, 2091
MslI CAYNNNNRTG 1 cut(s) 458
Msp20I TGGCCA 1 cut(s) 1668
MspA1I CMGCKG 2 cut(s) 472, 1604
MspI CCGG 1 cut(s) 1591
MspR9I CCNGG 6 cut(s) 759, 1095, 1143, 1665, 1737, 1889
MunI CAATTG 2 cut(s) 1843, 2124
Mva1269I GAATGC 2 cut(s) 308, 768
MvaI CCWGG 6 cut(s) 759, 1095, 1143, 1665, 1737, 1889
MwoI GCNNNNNNNGC 6 cut(s) 629, 763, 1410, 1419, 1529, 1625
NaeI GCCGGC 1 cut(s) 1592
NarI GGCGCC 1 cut(s) 318
NdeII GATC 6 cut(s) 102, 682, 1011, 1210, 1240, 1768
NgoMIV GCCGGC 1 cut(s) 1590
NlaIII CATG 9 cut(s) 251, 356, 1055, 1420, 1582, 1598, 1616, 1676, 2143
NlaIV GGNNCC 3 cut(s) 319, 671, 933
NmeAIII GCCGAG 1 cut(s) 27
NmuCI GTSAC 7 cut(s) 449, 614, 974, 1137, 1655, 2044, 2326
NspI RCATGY 4 cut(s) 251, 1055, 1582, 2143
PaeI GCATGC 1 cut(s) 1055
PaeR7I CTCGAG 1 cut(s) 1467
PciI ACATGT 3 cut(s) 247, 1578, 2139
PcsI WCGNNNNNNNCGW 1 cut(s) 2276
PctI GAATGC 2 cut(s) 308, 768
PdiI GCCGGC 1 cut(s) 1592
PfeI GAWTC 4 cut(s) 221, 638, 695, 1353
PfoI TCCNGGA 1 cut(s) 1093
PkrI GCNGC 8 cut(s) 307, 566, 1415, 1525, 1603, 1618, 1621, 2341
PleI GAGTC 6 cut(s) 377, 1343, 1427, 1652, 1797, 2168
PluTI GGCGCC 1 cut(s) 321
PpsI GAGTC 6 cut(s) 377, 1343, 1427, 1652, 1797, 2168
Ppu21I YACGTR 1 cut(s) 377
PscI ACATGT 3 cut(s) 247, 1578, 2139
Psp6I CCWGG 6 cut(s) 757, 1093, 1141, 1663, 1735, 1887
PspEI GGTNACC 1 cut(s) 614
PspGI CCWGG 6 cut(s) 757, 1093, 1141, 1663, 1735, 1887
PspN4I GGNNCC 3 cut(s) 319, 671, 933
PspPI GGNCC 2 cut(s) 31, 1420
PspXI VCTCGAGB 1 cut(s) 1467
PstI CTGCAG 1 cut(s) 1528
PstNI CAGNNNCTG 1 cut(s) 1513
PsuI RGATCY 2 cut(s) 102, 1210
PvuII CAGCTG 2 cut(s) 472, 1604
RsaI GTAC 7 cut(s) 202, 442, 517, 787, 862, 1134, 2144
RsaNI GTAC 7 cut(s) 201, 441, 516, 786, 861, 1133, 2143
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 8 cut(s) 150, 360, 392, 408, 813, 1281, 1689, 2091
SatI GCNGC 8 cut(s) 306, 565, 1414, 1524, 1602, 1617, 1620, 2340
Sau3AI GATC 6 cut(s) 102, 682, 1011, 1210, 1240, 1768
Sau96I GGNCC 2 cut(s) 31, 1420
SchI GAGTC 6 cut(s) 378, 1344, 1427, 1653, 1798, 2168
ScrFI CCNGG 6 cut(s) 759, 1095, 1143, 1665, 1737, 1889
SfaNI GCATC 4 cut(s) 744, 784, 1481, 2176
SfcI CTRYAG 3 cut(s) 624, 1524, 1585
SfoI GGCGCC 1 cut(s) 319
Sfr274I CTCGAG 1 cut(s) 1467
SlaI CTCGAG 1 cut(s) 1467
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 5 cut(s) 593, 992, 1367, 1467, 2245
SmoI CTYRAG 5 cut(s) 593, 992, 1367, 1467, 2245
SpeI ACTAGT 2 cut(s) 167, 2345
SphI GCATGC 1 cut(s) 1055
SsiI CCGC 3 cut(s) 621, 1641, 2340
SspDI GGCGCC 1 cut(s) 317
SspI AATATT 1 cut(s) 2110
SspMI CTAG 9 cut(s) 65, 156, 168, 482, 1347, 1623, 1728, 1899, 2346
StyD4I CCNGG 6 cut(s) 757, 1093, 1141, 1663, 1735, 1887
StyI CCWWGG 4 cut(s) 27, 313, 1549, 1849
TaaI ACNGT 6 cut(s) 16, 1137, 1717, 2080, 2168, 2326
TaiI ACGT 2 cut(s) 264, 379
TaqI TCGA 7 cut(s) 606, 681, 885, 1468, 1680, 1938, 2279
TatI WGTACW 5 cut(s) 440, 515, 785, 1132, 2142
TauI GCSGC 1 cut(s) 2342
TfiI GAWTC 4 cut(s) 221, 638, 695, 1353
Tru1I TTAA 8 cut(s) 150, 360, 392, 408, 813, 1281, 1689, 2091
Tru9I TTAA 8 cut(s) 150, 360, 392, 408, 813, 1281, 1689, 2091
TscAI CASTG 4 cut(s) 244, 1518, 1533, 2188
TseFI GTSAC 7 cut(s) 449, 614, 974, 1137, 1655, 2044, 2326
TseI GCWGC 7 cut(s) 305, 564, 1413, 1523, 1601, 1616, 1619
Tsp45I GTSAC 7 cut(s) 449, 614, 974, 1137, 1655, 2044, 2326
TspDTI ATGAA 3 cut(s) 48, 603, 716
TspGWI ACGGA 2 cut(s) 1263, 1839
TspRI CASTG 4 cut(s) 244, 1518, 1533, 2188
XapI RAATTY 6 cut(s) 554, 1018, 1147, 1807, 2055, 2297
XceI RCATGY 4 cut(s) 251, 1055, 1582, 2143
XhoI CTCGAG 1 cut(s) 1467
XspI CTAG 9 cut(s) 65, 156, 168, 482, 1347, 1623, 1728, 1899, 2346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.