Rh7AG122700

Galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000881
Physical Location & Seq
Reverse (-)
9619373 .. 9621564
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG122700.1

Sequence Viewer

Length: 1449 bp
ATGCATAGTTTTGTGGCCAGGTTGGCAAGCTTAGAAGAAAATGATAGGTTCAAGGGCTACCCCTCGGGAAAGTCATCATTTCCTAATCTCCGCCAGTTTGTGAAAACCATCAAAGAGGAACATGGACTGAAGTTAGTATATGCATGGCATGCTTTACTTGGTTCATGGGGAGGGCTACTTCCAACATCTGAAGCACTGAGGAAATACAATCCCCAAATCAAATCCATAGTTCAGTCTCCTGGTAACAAGAGTAACGTCATATGTGGAACCCTCGATCCAATGGAGAAACATGGGATCGGAATGATTGACCCTTCAATGATTTATAGATTTTACGATGATCTACATAGCTACCTTGCAAGTTGCAATATAGACGGAGTGAAGGTGGATATACACAATGAAGTGGAACTCCTTGCTTCTGGTTATGGAGGCCGCGTTGCATTGATGAGGCATTTCCAAGAAGCCCTCGAGGAATCTGTCATGAGAAACTTTGGCAGCAATAACCTTATTTGTTCCATGAGTCTAAGCAATGACTACATTTACAGCTCAAAGAAGAGTGCAGCTTCAAGAGTGTCAGAGGACTTCATGCCACTAGAGAAAACATTTCAGACACTACATGTTGCTGCAGTAGCTTTTAACAGTCTTCTTATGGGAGAGATTGTTGTACCAGATTGGGACATGTTCTTTAGCGACCACTACACCCGGGAGTTCCATGCTGCAGCCAGAGCATTGGGTGGATGTCCAGTATATGTAAGTGACAAGCCTGGCAGCCATAATTTCAATGTCCTCAAAAAGCTTGTACTGCCTGATGGCTCTATTCTAAGAGCTAGATTCGCTGGCCGGCCTACTAGGGACTGCTTATTCAGTGACCCTGTGGTCGATGGAAAAGGAGTTATTGGTGTTTTCAATTGCCAAAGAGCCGGAAAGTGGCCACCAATTGTAGGTGCTCAATATGAGCCTCCCTCTGACTCTGCACTGCCTCTTATAGGCCTCGTCAGCCCCATAGATATTAATATGCTTGAAGATGTTGCAAATGAAAGTTGGAGAGGAGAATGTGCAGTATATGCATACCATTCAGGTACTCTTTCTGTAATGCCAAAGAAAGACCACTTTGAAGTATCCTTGGATGTGCTAGAATGTGAAGTTTTCACCATATCTCCTATTATGGTTTTTGGTGACAACCTTCAATTTGCCCCAATGGGGTTGCTTGATATGTACAATTCAGGAGGAGCACTTGAATCTCTAGATGTCAGCAACAATGATCTGTTCGACTGTGTTGTGAAGGTTCGAGTCAGAGGATGTGGTCGATTTGGAGCCTACTCAAACAAAAAACCAAAGTCTTGTCTGGTGAACAAGAAAGAGGAGTTTATAGTCTACAATGCTAATAATGGTTTGTTGGTACTTAAACTTCAAGGCGACTGCAAAGTAAAAGAGATTGAGTTCATGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

482

Amino Acids

53.51

Weight (kDa)

6.13

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 3 - 468 6.2e-146 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 872
AbsI CCTCGAGG 1 cut(s) 464
AccI GTMKAC 1 cut(s) 1371
AccII CGCG 1 cut(s) 432
AciI CCGC 2 cut(s) 91, 430
AclWI GGATC 2 cut(s) 269, 302
AcoI YGGCCR 3 cut(s) 15, 835, 926
AcuI CTGAAG 2 cut(s) 149, 210
AfaI GTAC 6 cut(s) 663, 798, 1078, 1214, 1398, 1445
AfiI CCNNNNNNNGG 4 cut(s) 924, 938, 983, 1197
AflIII ACRYGT 2 cut(s) 613, 675
AjnI CCWGG 3 cut(s) 17, 238, 760
AloI GAACNNNNNNTCC 2 cut(s) 259, 291
AluBI AGCT 7 cut(s) 30, 348, 543, 560, 629, 793, 824
AluI AGCT 7 cut(s) 30, 348, 543, 560, 629, 793, 824
Alw21I GWGCWC 2 cut(s) 946, 1231
Alw26I GTCTC 1 cut(s) 240
AlwI GGATC 2 cut(s) 269, 302
Ama87I CYCGRG 3 cut(s) 64, 464, 699
AoxI GGCC 6 cut(s) 15, 427, 835, 839, 926, 985
ApeKI GCWGC 6 cut(s) 492, 557, 620, 713, 716, 765
AseI ATTAAT 1 cut(s) 1008
AsuC2I CCSGG 2 cut(s) 700, 701
AsuHPI GGTGA 3 cut(s) 1138, 1184, 1357
AvaI CYCGRG 3 cut(s) 64, 464, 699
BalI TGGCCA 2 cut(s) 17, 928
BarI GAAGNNNNNNTAC 2 cut(s) 1389, 1421
BbsI GAAGAC 1 cut(s) 632
Bbv12I GWGCWC 2 cut(s) 946, 1231
BbvI GCAGC 6 cut(s) 504, 569, 607, 700, 728, 777
BccI CCATC 3 cut(s) 116, 800, 872
BciT130I CCWGG 3 cut(s) 19, 240, 762
BciVI GTATCC 1 cut(s) 1126
BcnI CCSGG 2 cut(s) 700, 701
BcoDI GTCTC 1 cut(s) 240
BfaI CTAG 5 cut(s) 590, 825, 846, 1130, 1241
BfmI CTRYAG 2 cut(s) 621, 714
BfuI GTATCC 1 cut(s) 1126
BglI GCCNNNNNGGC 1 cut(s) 23
BisI GCNGC 7 cut(s) 430, 493, 558, 621, 714, 717, 766
BlsI GCNGC 7 cut(s) 431, 494, 559, 622, 715, 718, 767
Bme1390I CCNGG 5 cut(s) 19, 240, 700, 701, 762
BmeT110I CYCGRG 3 cut(s) 64, 464, 699
BmiI GGNNCC 2 cut(s) 268, 1312
BmrFI CCNGG 5 cut(s) 19, 240, 700, 701, 762
BpiI GAAGAC 1 cut(s) 632
BplI GAGNNNNNCTC 2 cut(s) 944, 976
BpuMI CCSGG 2 cut(s) 700, 701
BsaJI CCNNGG 3 cut(s) 63, 699, 1119
BsaXI ACNNNNNCTCC 8 cut(s) 366, 396, 417, 447, 642, 672, 1138, 1168
Bsc4I CCNNNNNNNGG 4 cut(s) 924, 938, 983, 1197
Bse118I RCCGGY 1 cut(s) 837
Bse1I ACTGG 2 cut(s) 94, 740
Bse3DI GCAATG 1 cut(s) 532
BseBI CCWGG 3 cut(s) 19, 240, 762
BseDI CCNNGG 3 cut(s) 63, 699, 1119
BseGI GGATG 3 cut(s) 740, 1129, 1301
BseLI CCNNNNNNNGG 4 cut(s) 924, 938, 983, 1197
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 1 cut(s) 188
BseNI ACTGG 2 cut(s) 94, 740
BseRI GAGGAG 3 cut(s) 1059, 1239, 1373
BseXI GCAGC 6 cut(s) 504, 569, 607, 700, 728, 777
BsgI GTGCAG 3 cut(s) 576, 954, 1074
Bsh1236I CGCG 1 cut(s) 432
BshFI GGCC 6 cut(s) 17, 429, 837, 841, 928, 987
BsiHKAI GWGCWC 2 cut(s) 946, 1231
BsiHKCI CYCGRG 3 cut(s) 64, 464, 699
BsiSI CCGG 3 cut(s) 700, 838, 918
BslFI GGGAC 2 cut(s) 686, 863
BslI CCNNNNNNNGG 4 cut(s) 924, 938, 983, 1197
BsmAI GTCTC 1 cut(s) 240
BsmFI GGGAC 2 cut(s) 686, 863
BsnI GGCC 6 cut(s) 17, 429, 837, 841, 928, 987
BsoBI CYCGRG 3 cut(s) 64, 464, 699
Bsp1286I GDGCHC 2 cut(s) 946, 1231
Bsp1407I TGTACA 1 cut(s) 1212
Bsp143I GATC 4 cut(s) 274, 294, 337, 1258
BspACI CCGC 2 cut(s) 91, 430
BspANI GGCC 6 cut(s) 17, 429, 837, 841, 928, 987
BspCNI CTCAG 1 cut(s) 189
BspFNI CGCG 1 cut(s) 432
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 2 cut(s) 268, 1312
BspMAI CTGCAG 2 cut(s) 625, 718
BspPI GGATC 2 cut(s) 269, 302
BsrDI GCAATG 1 cut(s) 532
BsrFI RCCGGY 1 cut(s) 837
BsrGI TGTACA 1 cut(s) 1212
BsrI ACTGG 2 cut(s) 94, 740
BssAI RCCGGY 1 cut(s) 837
BssECI CCNNGG 3 cut(s) 63, 699, 1119
BssMI GATC 4 cut(s) 274, 294, 337, 1258
BssT1I CCWWGG 1 cut(s) 1119
Bst2UI CCWGG 3 cut(s) 19, 240, 762
Bst4CI ACNGT 2 cut(s) 638, 1271
Bst6I CTCTTC 1 cut(s) 545
BstAPI GCANNNNNTGC 2 cut(s) 149, 1061
BstAUI TGTACA 1 cut(s) 1212
BstC8I GCNNGC 4 cut(s) 28, 150, 835, 839
BstDEI CTNAG 4 cut(s) 31, 197, 521, 818
BstENI CCTNNNNNAGG 1 cut(s) 981
BstF5I GGATG 3 cut(s) 740, 1129, 1301
BstFNI CGCG 1 cut(s) 432
BstKTI GATC 4 cut(s) 277, 297, 340, 1261
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 4 cut(s) 274, 294, 337, 1258
BstMWI GCNNNNNNNGC 8 cut(s) 23, 149, 626, 722, 799, 830, 993, 1061
BstNI CCWGG 3 cut(s) 19, 240, 762
BstNSI RCATGY 3 cut(s) 152, 617, 679
BstSCI CCNGG 5 cut(s) 17, 238, 698, 699, 760
BstSFI CTRYAG 2 cut(s) 621, 714
BstUI CGCG 1 cut(s) 432
BstV1I GCAGC 6 cut(s) 504, 569, 607, 700, 728, 777
BstV2I GAAGAC 1 cut(s) 632
BstXI CCANNNNNNTGG 1 cut(s) 727
BsuI GTATCC 1 cut(s) 1126
BsuRI GGCC 6 cut(s) 17, 429, 837, 841, 928, 987
BtsCI GGATG 3 cut(s) 740, 1129, 1301
BtsI GCAGTG 1 cut(s) 971
BtsIMutI CAGTG 3 cut(s) 194, 868, 971
Cac8I GCNNGC 4 cut(s) 28, 150, 835, 839
CciI TCATGA 1 cut(s) 477
Cfr10I RCCGGY 1 cut(s) 837
Cfr9I CCCGGG 1 cut(s) 699
Csp6I GTAC 6 cut(s) 662, 797, 1077, 1213, 1397, 1444
CviQI GTAC 6 cut(s) 662, 797, 1077, 1213, 1397, 1444
DdeI CTNAG 4 cut(s) 31, 197, 521, 818
DpnI GATC 4 cut(s) 276, 296, 339, 1260
DpnII GATC 4 cut(s) 274, 294, 337, 1258
DrdI GACNNNNNNGTC 1 cut(s) 872
DseDI GACNNNNNNGTC 1 cut(s) 872
EaeI YGGCCR 3 cut(s) 15, 835, 926
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
EciI GGCGGA 1 cut(s) 80
Eco130I CCWWGG 1 cut(s) 1119
Eco147I AGGCCT 1 cut(s) 987
Eco57I CTGAAG 2 cut(s) 149, 210
Eco88I CYCGRG 3 cut(s) 64, 464, 699
EcoNI CCTNNNNNAGG 1 cut(s) 981
EcoRII CCWGG 3 cut(s) 17, 238, 760
EcoT14I CCWWGG 1 cut(s) 1119
EcoT22I ATGCAT 3 cut(s) 6, 145, 1066
ErhI CCWWGG 1 cut(s) 1119
FaqI GGGAC 2 cut(s) 686, 863
FauNDI CATATG 1 cut(s) 260
FblI GTMKAC 1 cut(s) 1371
Fnu4HI GCNGC 7 cut(s) 430, 493, 558, 621, 714, 717, 766
FokI GGATG 3 cut(s) 747, 1136, 1308
FseI GGCCGGCC 1 cut(s) 841
Fsp4HI GCNGC 7 cut(s) 430, 493, 558, 621, 714, 717, 766
FspBI CTAG 5 cut(s) 590, 825, 846, 1130, 1241
GluI GCNGC 7 cut(s) 430, 493, 558, 621, 714, 717, 766
HaeIII GGCC 6 cut(s) 17, 429, 837, 841, 928, 987
HapII CCGG 3 cut(s) 700, 838, 918
HindIII AAGCTT 2 cut(s) 28, 791
HinfI GANTC 6 cut(s) 470, 517, 828, 965, 1235, 1287
HpaII CCGG 3 cut(s) 700, 838, 918
HphI GGTGA 3 cut(s) 1138, 1184, 1357
Hpy166II GTNNAC 2 cut(s) 1348, 1372
Hpy188I TCNGA 6 cut(s) 190, 299, 574, 606, 964, 1292
Hpy188III TCNNGA 5 cut(s) 66, 478, 564, 1221, 1241
Hpy8I GTNNAC 2 cut(s) 1348, 1372
HpyAV CCTTC 4 cut(s) 321, 373, 1190, 1273
HpyCH4III ACNGT 2 cut(s) 638, 1271
HpyCH4IV ACGT 1 cut(s) 255
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 149, 626, 722, 799, 830, 993, 1061
HpyF3I CTNAG 4 cut(s) 31, 197, 521, 818
HpySE526I ACGT 1 cut(s) 255
KroI GCCGGC 1 cut(s) 837
KroNI GCCGGC 1 cut(s) 839
Kzo9I GATC 4 cut(s) 274, 294, 337, 1258
LmnI GCTCC 2 cut(s) 1226, 1310
Lsp1109I GCAGC 6 cut(s) 504, 569, 607, 700, 728, 777
MaeI CTAG 5 cut(s) 590, 825, 846, 1130, 1241
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 5 cut(s) 242, 251, 752, 863, 1172
MalI GATC 4 cut(s) 276, 296, 339, 1260
MboI GATC 4 cut(s) 274, 294, 337, 1258
MboII GAAGA 4 cut(s) 47, 562, 632, 1031
MfeI CAATTG 2 cut(s) 904, 933
MhlI GDGCHC 2 cut(s) 946, 1231
MlsI TGGCCA 2 cut(s) 17, 928
MluCI AATT 5 cut(s) 772, 904, 933, 1184, 1216
MluNI TGGCCA 2 cut(s) 17, 928
MlyI GAGTC 3 cut(s) 526, 959, 1296
MmeI TCCRAC 2 cut(s) 206, 1019
Mox20I TGGCCA 2 cut(s) 17, 928
Mph1103I ATGCAT 3 cut(s) 6, 145, 1066
MroNI GCCGGC 1 cut(s) 837
MscI TGGCCA 2 cut(s) 17, 928
MseI TTAA 3 cut(s) 633, 1008, 1401
Msp20I TGGCCA 2 cut(s) 17, 928
MspI CCGG 3 cut(s) 700, 838, 918
MspR9I CCNGG 5 cut(s) 19, 240, 700, 701, 762
MunI CAATTG 2 cut(s) 904, 933
MvaI CCWGG 3 cut(s) 19, 240, 762
MvnI CGCG 1 cut(s) 432
MwoI GCNNNNNNNGC 8 cut(s) 23, 149, 626, 722, 799, 830, 993, 1061
NaeI GCCGGC 1 cut(s) 839
NciI CCSGG 2 cut(s) 700, 701
NdeI CATATG 1 cut(s) 260
NdeII GATC 4 cut(s) 274, 294, 337, 1258
NgoMIV GCCGGC 1 cut(s) 837
NlaIV GGNNCC 2 cut(s) 268, 1312
NmuCI GTSAC 3 cut(s) 752, 863, 1172
NsiI ATGCAT 3 cut(s) 6, 145, 1066
NspI RCATGY 3 cut(s) 152, 617, 679
PaeI GCATGC 1 cut(s) 152
PaeR7I CTCGAG 1 cut(s) 464
PagI TCATGA 1 cut(s) 477
PceI AGGCCT 1 cut(s) 987
PciI ACATGT 2 cut(s) 613, 675
PdiI GCCGGC 1 cut(s) 839
PfeI GAWTC 3 cut(s) 470, 828, 1235
PkrI GCNGC 7 cut(s) 431, 494, 559, 622, 715, 718, 767
PleI GAGTC 3 cut(s) 525, 959, 1295
PpsI GAGTC 3 cut(s) 525, 959, 1295
PscI ACATGT 2 cut(s) 613, 675
PshBI ATTAAT 1 cut(s) 1008
Psp6I CCWGG 3 cut(s) 17, 238, 760
PspGI CCWGG 3 cut(s) 17, 238, 760
PspN4I GGNNCC 2 cut(s) 268, 1312
PspXI VCTCGAGB 1 cut(s) 464
PstI CTGCAG 2 cut(s) 625, 718
RigI GGCCGGCC 1 cut(s) 841
RsaI GTAC 6 cut(s) 663, 798, 1078, 1214, 1398, 1445
RsaNI GTAC 6 cut(s) 662, 797, 1077, 1213, 1397, 1444
SaqAI TTAA 3 cut(s) 633, 1008, 1401
SatI GCNGC 7 cut(s) 430, 493, 558, 621, 714, 717, 766
Sau3AI GATC 4 cut(s) 274, 294, 337, 1258
SchI GAGTC 3 cut(s) 526, 959, 1296
ScrFI CCNGG 5 cut(s) 19, 240, 700, 701, 762
SduI GDGCHC 2 cut(s) 946, 1231
SfcI CTRYAG 2 cut(s) 621, 714
Sfr274I CTCGAG 1 cut(s) 464
SlaI CTCGAG 1 cut(s) 464
SmaI CCCGGG 1 cut(s) 701
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
SphI GCATGC 1 cut(s) 152
Sse9I AATT 5 cut(s) 772, 904, 933, 1184, 1216
SseBI AGGCCT 1 cut(s) 987
SsiI CCGC 2 cut(s) 91, 430
SspMI CTAG 5 cut(s) 590, 825, 846, 1130, 1241
StuI AGGCCT 1 cut(s) 987
StyD4I CCNGG 5 cut(s) 17, 238, 698, 699, 760
StyI CCWWGG 1 cut(s) 1119
TaaI ACNGT 2 cut(s) 638, 1271
TaiI ACGT 1 cut(s) 258
TaqI TCGA 6 cut(s) 273, 465, 876, 1266, 1285, 1303
TasI AATT 5 cut(s) 772, 904, 933, 1184, 1216
TatI WGTACW 3 cut(s) 796, 1212, 1443
TauI GCSGC 1 cut(s) 432
TfiI GAWTC 3 cut(s) 470, 828, 1235
Tru1I TTAA 3 cut(s) 633, 1008, 1401
Tru9I TTAA 3 cut(s) 633, 1008, 1401
TscAI CASTG 3 cut(s) 201, 868, 978
TseFI GTSAC 3 cut(s) 752, 863, 1172
TseI GCWGC 6 cut(s) 492, 557, 620, 713, 716, 765
Tsp45I GTSAC 3 cut(s) 752, 863, 1172
TspDTI ATGAA 5 cut(s) 153, 411, 571, 1047, 1429
TspGWI ACGGA 1 cut(s) 387
TspMI CCCGGG 1 cut(s) 699
TspRI CASTG 3 cut(s) 201, 868, 978
VspI ATTAAT 1 cut(s) 1008
XagI CCTNNNNNAGG 1 cut(s) 981
XbaI TCTAGA 1 cut(s) 1240
XceI RCATGY 3 cut(s) 152, 617, 679
XhoI CTCGAG 1 cut(s) 464
XmaI CCCGGG 1 cut(s) 699
XmiI GTMKAC 1 cut(s) 1371
XspI CTAG 5 cut(s) 590, 825, 846, 1130, 1241
Zsp2I ATGCAT 3 cut(s) 6, 145, 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.