Rh5BG002800

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000881
Physical Location & Seq
Reverse (-)
280075 .. 285758
5684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG002800.1

Sequence Viewer

Length: 2451 bp
ATGCTTCACAGCACCGTCGTTTCCCCCAAGGCCCTTCAACTCAATCTCGGCTTCTCTTCATTTCTAGCCACAAACCAGAACCAGAGCAGGAGGACAAACCAGAACCAGAGCAGGAGGAGGAGGAGGATCATCTTTTGCTCACAAACCAGAGATGGTTCTGTGCGTGGTGGAGTTAAGACTAGCAGCACTACCAGTAGTAATGGATGGAGACAATCTATGTTTGTCGGTACGAAACCTGCCCTTGAAGATTCCATTCTGAGTGTCAGTGGTAAACATGTGTTGACAGACGTGCCTCCCAATGTGGTCTTCACACCAATTCCCTACTCATCGGCAGCATTCCTTGGCGCCACTTCTCAAAATGCTACTTCACAATCACGCCATGTTTTTAAGCTCGGAGTCTTACGTGATGTCAGATTATTAAGTCTTTTTAGATTTAAACTTTGGTGGATGATACCACGAGTGGGCAGTACAGGAAGTGACATCCCTGTGGAAACTCAGCTGTTGCTCCTAGAAGCAAAGGAGGAGGAGGGAAAGGAAGATAGTACATCTTATATCTTATTCTTGCCAGTTCTGGATGGTGAATTTAGAAGCAGCTTGCAGGGAAATACATCCAATGAACTTGAGTTTTGCGTCGAAAGTGGTGACCCCGCTATAGTTGCCTCAGAATCCCTAAAAGCAGTTTTTGTGAATTGTGGGAACCATCCTTTCGATCTGGTGAACGAATCTATGAAGACTTTGGCGAAGCATTATGGAAGCTTTGCTCTTAGAGAAACGAAACAGATGCCTGGAATGCTGGATTGTTTTGGCTGGTGTACTTGGGATGCCTTTTATCAAGGAGTTAACCCTAAAGGAATTAGAGACGGCCTCAGAAGTTTATCAGAAGGAGGTACTCCAGCTAAGTTTTTGATAATCGATGATGGGTGGCAAGATACATCAAATGAGTTCCAAAAAGAAGGGGAGCCATTTGTTGAAGGGACACAGTTTGGAGGCAGATTAAATAGCATTGAAGAAAATAACAAGTTTCGCAGCACAACCAATATGGTTGATAGTGACAAACCAAGTGGTCTCAAGGATTTTGTTTCTGAGATCAAGAATTCCTTTGGCCTTAGGTATGTGTATGTATGGCATGCCCTTTTGGGATATTGGGGAGGGCTTGTTCCAAATGCTCCAGGAACTAAAAAGTATAACACTAAGTTCAGACACCCAGTACAGTCACCTGGAAATTTGTCAAATATGAGGGATGGAGCTATGGATAGTATGGAGAAATATGGTGTTGGTGTAATAGATCCTGCAAAGGCATATCAATTTTATGATGATCTACACGGATATCTTGTTTCACAGGATGTAGATGGTGTTAAGGTTGATGTTCAGAACATACTGGAGACAGTTTCAGCAGGTTTAGGAGGGCGAGTCTCTCTAACTAGACGATTCCAACAGGCACTTGAGAAGTCCATAGCCACTCATTTCCAAGATAACAGCATAATCTGCTGCATGGGCCAAAGCACAGACTCCATTTACCATTCAAAAAGAAGTGCTATTACTCGAGCATCTGATGATTACTACCCAAAAAACCCAACAACACAGACACTGCACATTGCTGCAGTGGCTTTCAACAGCATATTCCTTGGTGAAGTTTTTGTCCCAGATTGGGACATGTTCTATAGCCGGCATGAGGCAGCTGAGTTCCATGCTGCTGCTAGAGCTGTGGGAGGTTGCGGAGTCTATGTAAGTGACAAACCTGGCCAGCATGATTTCGAGATACTTAAAAGGCTTGTTCTTCCTGATGGAACAGTTCTCAGAGCTAGATATCCTGGGAGGCCATCACGGGATTGTTTATTTGTTGATCCAGTTATGGACGGGAAGAGTCTTCTGAAAATATGGAATTTGAACAAATGCAACGGAGTTATAGGCATTTTCAATTGCCAAGGAGCAGGAAGCTGGCCTTGTCTGGAACATATCGTCCAGGTAAAAGCTAGTGAGGAGCTATCTGGGAAGGTCTCTCCAGCCGATATCGAGTATTTTGAAGAGGTATCTGGAAAGCTTTGGACTGGAGATTGTGCAGTCTATTCCTTCAAACAAGGGTATCTGTCTCGTTTACCGAAGGACAAGTCAGTTGATGTCACATTACAAATTCTCCAATGCGATGTGTTTACTGTATCCCCTATTAAGGTTTACAAGCAAAATATTCAATTTGCACCAATTGGATTATTGAACATGTACAATTCCGGTGGAGCTGTTGACAGTATTGACTTGTTCAGTGATGCCTCTAGTTGTGTTATACACATCAAGGGAAGAGGGGCTGGTAGCTTTGGAGCGTACTCAAGTTCAAAGCCCAAATCTTGCTCGGTGAACTCGAAAGATGAGGGATTTGAATTCAGAGGTGATGACAATCTTCTGACTGTGACAATTCCTGCCGCAACTAGTTCTTGGAGCATTAGTTTCTCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

816

Amino Acids

90.04

Weight (kDa)

6.77

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 82 - 802 1.3e-264 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 244, 1385
AccB1I GGYRCC 1 cut(s) 344
AciI CCGC 3 cut(s) 648, 1716, 2415
AclWI GGATC 3 cut(s) 134, 1280, 1838
AcoI YGGCCR 1 cut(s) 1741
AcsI RAATTY 6 cut(s) 581, 1093, 1222, 1882, 2130, 2372
AcyI GRCGYC 1 cut(s) 345
AfaI GTAC 8 cut(s) 229, 469, 544, 814, 889, 1209, 2219, 2318
AfiI CCNNNNNNNGG 4 cut(s) 461, 1648, 1672, 2167
AflIII ACRYGT 3 cut(s) 274, 1653, 2214
AhlI ACTAGT 1 cut(s) 2420
AjiI CACGTC 1 cut(s) 289
AjnI CCWGG 6 cut(s) 784, 1168, 1216, 1738, 1810, 1962
AjuI GAANNNNNNNTTGG 2 cut(s) 290, 322
AloI GAACNNNNNNTCC 4 cut(s) 1140, 1172, 1944, 1976
Alw26I GTCTC 7 cut(s) 202, 852, 1070, 1376, 1417, 2002, 2094
AlwI GGATC 3 cut(s) 134, 1280, 1838
AlwNI CAGNNNCTG 1 cut(s) 1588
Ama87I CYCGRG 1 cut(s) 1542
AoxI GGCC 7 cut(s) 30, 862, 1102, 1495, 1741, 1817, 1940
ApeKI GCWGC 9 cut(s) 183, 332, 591, 1026, 1488, 1598, 1676, 1691, 1694
ApoI RAATTY 6 cut(s) 581, 1093, 1222, 1882, 2130, 2372
ArsI GACNNNNNNTTYG 2 cut(s) 2093, 2125
AspLEI GCGC 1 cut(s) 347
AspS9I GGNCC 2 cut(s) 31, 1495
AsuHPI GGTGA 7 cut(s) 590, 653, 727, 1206, 1640, 2359, 2393
AvaI CYCGRG 1 cut(s) 1542
AxyI CCTNAGG 1 cut(s) 1106
BaeI ACNNNNGTAYC 2 cut(s) 2066, 2099
BalI TGGCCA 1 cut(s) 1743
BanI GGYRCC 1 cut(s) 344
BarI GAAGNNNNNNTAC 2 cut(s) 1522, 1554
BauI CACGAG 1 cut(s) 456
BbsI GAAGAC 3 cut(s) 298, 737, 1859
BbvI GCAGC 9 cut(s) 195, 344, 603, 1038, 1475, 1585, 1678, 1681, 1688
BccI CCATC 9 cut(s) 146, 198, 569, 708, 911, 1235, 1343, 1778, 1828
BceAI ACGGC 1 cut(s) 877
BcgI CGANNNNNNTGC 2 cut(s) 763, 797
BciT130I CCWGG 6 cut(s) 786, 1170, 1218, 1740, 1812, 1964
BciVI GTATCC 1 cut(s) 2167
BcoDI GTCTC 7 cut(s) 202, 852, 1070, 1376, 1417, 2002, 2094
BcuI ACTAGT 1 cut(s) 2420
BfaI CTAG 9 cut(s) 65, 180, 509, 1422, 1698, 1803, 1974, 2268, 2421
BfmI CTRYAG 3 cut(s) 651, 1599, 1660
BfoI RGCGCY 1 cut(s) 348
BfuAI ACCTGC 2 cut(s) 244, 1385
BfuI GTATCC 1 cut(s) 2167
Bme1390I CCNGG 6 cut(s) 786, 1170, 1218, 1740, 1812, 1964
BmeT110I CYCGRG 1 cut(s) 1542
BmgBI CACGTC 1 cut(s) 289
BmgT120I GGNCC 2 cut(s) 31, 1495
BmiI GGNNCC 3 cut(s) 346, 698, 960
BmrFI CCNGG 6 cut(s) 786, 1170, 1218, 1740, 1812, 1964
BmrI ACTGGG 1 cut(s) 1199
BmsI GCATC 4 cut(s) 771, 811, 1556, 2251
BmuI ACTGGG 1 cut(s) 1199
BpiI GAAGAC 3 cut(s) 298, 737, 1859
BplI GAGNNNNNCTC 2 cut(s) 849, 881
BpmI CTGGAG 5 cut(s) 876, 1152, 1400, 1986, 2070
BpuEI CTTGAG 4 cut(s) 641, 1052, 1463, 2305
Bsa29I ATCGAT 1 cut(s) 912
BsaAI YACGTR 1 cut(s) 404
BsaBI GATNNNNATC 1 cut(s) 2388
BsaHI GRCGYC 1 cut(s) 345
BsaI GGTCTC 2 cut(s) 1070, 2002
BsaJI CCNNGG 5 cut(s) 27, 340, 1624, 1811, 1924
BsaWI WCCGGW 1 cut(s) 2225
BsaXI ACNNNNNCTCC 9 cut(s) 1140, 1170, 1395, 1425, 1710, 1740, 2118, 2148, 2422
Bsc4I CCNNNNNNNGG 4 cut(s) 461, 1648, 1672, 2167
Bse118I RCCGGY 1 cut(s) 1665
Bse1I ACTGG 6 cut(s) 192, 566, 1205, 1383, 1847, 2053
Bse21I CCTNAGG 1 cut(s) 1106
Bse3DI GCAATG 1 cut(s) 1593
Bse8I GATNNNNATC 1 cut(s) 2388
BseBI CCWGG 6 cut(s) 786, 1170, 1218, 1740, 1812, 1964
BseCI ATCGAT 1 cut(s) 912
BseDI CCNNGG 5 cut(s) 27, 340, 1624, 1811, 1924
BseGI GGATG 9 cut(s) 209, 453, 480, 580, 608, 700, 826, 1246, 1348
BseJI GATNNNNATC 1 cut(s) 2388
BseLI CCNNNNNNNGG 4 cut(s) 461, 1648, 1672, 2167
BseMI GCAATG 1 cut(s) 1593
BseMII CTCAG 7 cut(s) 248, 509, 675, 880, 1074, 1671, 1810
BseNI ACTGG 6 cut(s) 192, 566, 1205, 1383, 1847, 2053
BseRI GAGGAG 6 cut(s) 130, 133, 136, 536, 539, 1994
BseXI GCAGC 9 cut(s) 195, 344, 603, 1038, 1475, 1585, 1678, 1681, 1688
BsgI GTGCAG 2 cut(s) 1574, 2079
BshFI GGCC 7 cut(s) 32, 864, 1104, 1497, 1743, 1819, 1942
BshNI GGYRCC 1 cut(s) 344
BshVI ATCGAT 1 cut(s) 912
BsiHKCI CYCGRG 1 cut(s) 1542
BsiSI CCGG 2 cut(s) 1666, 2226
BslFI GGGAC 3 cut(s) 988, 1625, 1664
BslI CCNNNNNNNGG 4 cut(s) 461, 1648, 1672, 2167
BsmAI GTCTC 7 cut(s) 202, 852, 1070, 1376, 1417, 2002, 2094
BsmBI CGTCTC 1 cut(s) 852
BsmFI GGGAC 3 cut(s) 988, 1625, 1664
BsmI GAATGC 2 cut(s) 335, 795
BsnI GGCC 7 cut(s) 32, 864, 1104, 1497, 1743, 1819, 1942
Bso31I GGTCTC 2 cut(s) 1070, 2002
BsoBI CYCGRG 1 cut(s) 1542
Bsp1407I TGTACA 1 cut(s) 2217
Bsp143I GATC 6 cut(s) 126, 709, 1086, 1285, 1315, 1843
BspACI CCGC 3 cut(s) 648, 1716, 2415
BspANI GGCC 7 cut(s) 32, 864, 1104, 1497, 1743, 1819, 1942
BspCNI CTCAG 7 cut(s) 249, 508, 674, 879, 1075, 1672, 1809
BspDI ATCGAT 1 cut(s) 912
BspLI GGNNCC 3 cut(s) 346, 698, 960
BspMAI CTGCAG 1 cut(s) 1603
BspMI ACCTGC 2 cut(s) 244, 1385
BspPI GGATC 3 cut(s) 134, 1280, 1838
BspT107I GGYRCC 1 cut(s) 344
BspTNI GGTCTC 2 cut(s) 1070, 2002
BsrDI GCAATG 1 cut(s) 1593
BsrFI RCCGGY 1 cut(s) 1665
BsrGI TGTACA 1 cut(s) 2217
BsrI ACTGG 6 cut(s) 192, 566, 1205, 1383, 1847, 2053
BssAI RCCGGY 1 cut(s) 1665
BssECI CCNNGG 5 cut(s) 27, 340, 1624, 1811, 1924
BssMI GATC 6 cut(s) 126, 709, 1086, 1285, 1315, 1843
BssNI GRCGYC 1 cut(s) 345
BssSI CACGAG 1 cut(s) 456
BssT1I CCWWGG 4 cut(s) 27, 340, 1624, 1924
Bst2BI CACGAG 1 cut(s) 456
Bst2UI CCWGG 6 cut(s) 786, 1170, 1218, 1740, 1812, 1964
Bst4CI ACNGT 8 cut(s) 16, 981, 1212, 1387, 1792, 2155, 2243, 2401
Bst6I CTCTTC 4 cut(s) 61, 1856, 2019, 2287
BstACI GRCGYC 1 cut(s) 345
BstAPI GCANNNNNTGC 1 cut(s) 1485
BstAUI TGTACA 1 cut(s) 2217
BstBAI YACGTR 1 cut(s) 404
BstC8I GCNNGC 5 cut(s) 596, 1128, 1667, 1745, 1940
BstEII GGTNACC 1 cut(s) 641
BstF5I GGATG 9 cut(s) 209, 453, 480, 580, 608, 700, 826, 1246, 1348
BstH2I RGCGCY 1 cut(s) 348
BstHHI GCGC 1 cut(s) 347
BstKTI GATC 6 cut(s) 129, 712, 1089, 1288, 1318, 1846
BstMAI GTCTC 7 cut(s) 202, 852, 1070, 1376, 1417, 2002, 2094
BstMBI GATC 6 cut(s) 126, 709, 1086, 1285, 1315, 1843
BstMWI GCNNNNNNNGC 6 cut(s) 656, 790, 1485, 1494, 1604, 1700
BstNI CCWGG 6 cut(s) 786, 1170, 1218, 1740, 1812, 1964
BstNSI RCATGY 4 cut(s) 278, 1130, 1657, 2218
BstPI GGTNACC 1 cut(s) 641
BstSCI CCNGG 6 cut(s) 784, 1168, 1216, 1738, 1810, 1962
BstSFI CTRYAG 3 cut(s) 651, 1599, 1660
BstV1I GCAGC 9 cut(s) 195, 344, 603, 1038, 1475, 1585, 1678, 1681, 1688
BstV2I GAAGAC 3 cut(s) 298, 737, 1859
BstX2I RGATCY 1 cut(s) 1285
BstYI RGATCY 1 cut(s) 1285
Bsu15I ATCGAT 1 cut(s) 912
Bsu36I CCTNAGG 1 cut(s) 1106
BsuI GTATCC 1 cut(s) 2167
BsuRI GGCC 7 cut(s) 32, 864, 1104, 1497, 1743, 1819, 1942
BsuTUI ATCGAT 1 cut(s) 912
BtgZI GCGATG 1 cut(s) 2157
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 9 cut(s) 209, 453, 480, 580, 608, 700, 826, 1246, 1348
BtsI GCAGTG 2 cut(s) 1586, 1608
BtsIMutI CAGTG 4 cut(s) 271, 1586, 1608, 2263
BveI ACCTGC 2 cut(s) 244, 1385
Cac8I GCNNGC 5 cut(s) 596, 1128, 1667, 1745, 1940
CaiI CAGNNNCTG 1 cut(s) 1588
CfoI GCGC 1 cut(s) 347
Cfr10I RCCGGY 1 cut(s) 1665
Cfr13I GGNCC 2 cut(s) 31, 1495
ClaI ATCGAT 1 cut(s) 912
CseI GACGC 1 cut(s) 619
Csp6I GTAC 8 cut(s) 228, 468, 543, 813, 888, 1208, 2218, 2317
CspCI CAANNNNNGTGG 4 cut(s) 1042, 1077, 2209, 2244
CviAII CATG 9 cut(s) 275, 380, 1127, 1492, 1654, 1670, 1688, 1748, 2215
CviQI GTAC 8 cut(s) 228, 468, 543, 813, 888, 1208, 2218, 2317
DinI GGCGCC 1 cut(s) 346
DpnI GATC 6 cut(s) 128, 711, 1088, 1287, 1317, 1845
DpnII GATC 6 cut(s) 126, 709, 1086, 1285, 1315, 1843
DraI TTTAAA 1 cut(s) 436
EaeI YGGCCR 1 cut(s) 1741
Eam1104I CTCTTC 4 cut(s) 61, 1856, 2019, 2287
EarI CTCTTC 4 cut(s) 61, 1856, 2019, 2287
Eco130I CCWWGG 4 cut(s) 27, 340, 1624, 1924
Eco31I GGTCTC 2 cut(s) 1070, 2002
Eco32I GATATC 3 cut(s) 1328, 1808, 2011
Eco81I CCTNAGG 1 cut(s) 1106
Eco88I CYCGRG 1 cut(s) 1542
Eco91I GGTNACC 1 cut(s) 641
EcoO109I RGGNCCY 1 cut(s) 31
EcoO65I GGTNACC 1 cut(s) 641
EcoRI GAATTC 2 cut(s) 1093, 2372
EcoRII CCWGG 6 cut(s) 784, 1168, 1216, 1738, 1810, 1962
EcoRV GATATC 3 cut(s) 1328, 1808, 2011
EcoT14I CCWWGG 4 cut(s) 27, 340, 1624, 1924
EgeI GGCGCC 1 cut(s) 346
EheI GGCGCC 1 cut(s) 346
ErhI CCWWGG 4 cut(s) 27, 340, 1624, 1924
Esp3I CGTCTC 1 cut(s) 852
FaeI CATG 9 cut(s) 278, 383, 1130, 1495, 1657, 1673, 1691, 1751, 2218
FalI AAGNNNNNCTT 4 cut(s) 1082, 1114, 1927, 1959
FaqI GGGAC 3 cut(s) 988, 1625, 1664
FatI CATG 9 cut(s) 274, 379, 1126, 1491, 1653, 1669, 1687, 1747, 2214
FauI CCCGC 1 cut(s) 655
FokI GGATG 9 cut(s) 216, 460, 467, 587, 595, 687, 833, 1253, 1355
FspBI CTAG 9 cut(s) 65, 180, 509, 1422, 1698, 1803, 1974, 2268, 2421
GlaI GCGC 1 cut(s) 346
GsuI CTGGAG 5 cut(s) 876, 1152, 1400, 1986, 2070
HaeII RGCGCY 1 cut(s) 348
HaeIII GGCC 7 cut(s) 32, 864, 1104, 1497, 1743, 1819, 1942
HapII CCGG 2 cut(s) 1666, 2226
HgaI GACGC 1 cut(s) 619
HhaI GCGC 1 cut(s) 347
Hin1I GRCGYC 1 cut(s) 345
Hin1II CATG 9 cut(s) 278, 383, 1130, 1495, 1657, 1673, 1691, 1751, 2218
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HincII GTYRAC 3 cut(s) 282, 841, 2239
HindII GTYRAC 3 cut(s) 282, 841, 2239
HindIII AAGCTT 2 cut(s) 754, 2039
HinfI GANTC 9 cut(s) 248, 396, 665, 722, 1410, 1428, 1508, 1719, 1864
HpaI GTTAAC 1 cut(s) 841
HpaII CCGG 2 cut(s) 1666, 2226
HphI GGTGA 7 cut(s) 590, 653, 727, 1206, 1640, 2359, 2393
Hpy188III TCNNGA 6 cut(s) 572, 1090, 1756, 1781, 1949, 2034
Hpy99I CGWCG 2 cut(s) 20, 635
HpyAV CCTTC 7 cut(s) 44, 875, 947, 965, 1987, 2080, 2095
HpyCH4III ACNGT 8 cut(s) 16, 981, 1212, 1387, 1792, 2155, 2243, 2401
HpyCH4IV ACGT 2 cut(s) 288, 403
HpyCH4V TGCA 8 cut(s) 598, 1292, 1491, 1591, 1601, 1896, 2060, 2195
HpyF10VI GCNNNNNNNGC 6 cut(s) 656, 790, 1485, 1494, 1604, 1700
HpySE526I ACGT 2 cut(s) 288, 403
Hsp92I GRCGYC 1 cut(s) 345
Hsp92II CATG 9 cut(s) 278, 383, 1130, 1495, 1657, 1673, 1691, 1751, 2218
HspAI GCGC 1 cut(s) 345
KasI GGCGCC 1 cut(s) 344
KroI GCCGGC 1 cut(s) 1665
KroNI GCCGGC 1 cut(s) 1667
KspAI GTTAAC 1 cut(s) 841
Kzo9I GATC 6 cut(s) 126, 709, 1086, 1285, 1315, 1843
LmnI GCTCC 9 cut(s) 510, 958, 1171, 1244, 1928, 1981, 2231, 2312, 2430
Lsp1109I GCAGC 9 cut(s) 195, 344, 603, 1038, 1475, 1585, 1678, 1681, 1688
LweI GCATC 4 cut(s) 771, 811, 1556, 2251
MaeI CTAG 9 cut(s) 65, 180, 509, 1422, 1698, 1803, 1974, 2268, 2421
MaeII ACGT 2 cut(s) 288, 403
MaeIII GTNAC 7 cut(s) 476, 641, 1049, 1212, 1730, 2119, 2401
MalI GATC 6 cut(s) 128, 711, 1088, 1287, 1317, 1845
MboI GATC 6 cut(s) 126, 709, 1086, 1285, 1315, 1843
MfeI CAATTG 2 cut(s) 1918, 2199
MflI RGATCY 1 cut(s) 1285
MlsI TGGCCA 1 cut(s) 1743
MluNI TGGCCA 1 cut(s) 1743
Mly113I GGCGCC 1 cut(s) 345
MlyI GAGTC 5 cut(s) 405, 1419, 1502, 1728, 1873
MmeI TCCRAC 1 cut(s) 1456
Mox20I TGGCCA 1 cut(s) 1743
MroNI GCCGGC 1 cut(s) 1665
MscI TGGCCA 1 cut(s) 1743
MseI TTAA 9 cut(s) 174, 387, 419, 435, 840, 995, 1356, 1764, 2166
MslI CAYNNNNRTG 1 cut(s) 485
Msp20I TGGCCA 1 cut(s) 1743
MspA1I CMGCKG 2 cut(s) 499, 1679
MspI CCGG 2 cut(s) 1666, 2226
MspR9I CCNGG 6 cut(s) 786, 1170, 1218, 1740, 1812, 1964
MunI CAATTG 2 cut(s) 1918, 2199
Mva1269I GAATGC 2 cut(s) 335, 795
MvaI CCWGG 6 cut(s) 786, 1170, 1218, 1740, 1812, 1964
MwoI GCNNNNNNNGC 6 cut(s) 656, 790, 1485, 1494, 1604, 1700
NaeI GCCGGC 1 cut(s) 1667
NarI GGCGCC 1 cut(s) 345
NdeII GATC 6 cut(s) 126, 709, 1086, 1285, 1315, 1843
NgoMIV GCCGGC 1 cut(s) 1665
NlaIII CATG 9 cut(s) 278, 383, 1130, 1495, 1657, 1673, 1691, 1751, 2218
NlaIV GGNNCC 3 cut(s) 346, 698, 960
NmeAIII GCCGAG 1 cut(s) 27
NmuCI GTSAC 7 cut(s) 476, 641, 1049, 1212, 1730, 2119, 2401
NspI RCATGY 4 cut(s) 278, 1130, 1657, 2218
PaeI GCATGC 1 cut(s) 1130
PaeR7I CTCGAG 1 cut(s) 1542
PciI ACATGT 3 cut(s) 274, 1653, 2214
PcsI WCGNNNNNNNCGW 1 cut(s) 2351
PctI GAATGC 2 cut(s) 335, 795
PdiI GCCGGC 1 cut(s) 1667
PfeI GAWTC 4 cut(s) 248, 665, 722, 1428
PfoI TCCNGGA 1 cut(s) 1168
PleI GAGTC 5 cut(s) 404, 1418, 1502, 1727, 1872
PluTI GGCGCC 1 cut(s) 348
PpsI GAGTC 5 cut(s) 404, 1418, 1502, 1727, 1872
Ppu21I YACGTR 1 cut(s) 404
PscI ACATGT 3 cut(s) 274, 1653, 2214
Psp6I CCWGG 6 cut(s) 784, 1168, 1216, 1738, 1810, 1962
PspEI GGTNACC 1 cut(s) 641
PspGI CCWGG 6 cut(s) 784, 1168, 1216, 1738, 1810, 1962
PspN4I GGNNCC 3 cut(s) 346, 698, 960
PspPI GGNCC 2 cut(s) 31, 1495
PspXI VCTCGAGB 1 cut(s) 1542
PstI CTGCAG 1 cut(s) 1603
PstNI CAGNNNCTG 1 cut(s) 1588
PsuI RGATCY 1 cut(s) 1285
PvuII CAGCTG 2 cut(s) 499, 1679
RsaI GTAC 8 cut(s) 229, 469, 544, 814, 889, 1209, 2219, 2318
RsaNI GTAC 8 cut(s) 228, 468, 543, 813, 888, 1208, 2218, 2317
RseI CAYNNNNRTG 1 cut(s) 485
SaqAI TTAA 9 cut(s) 174, 387, 419, 435, 840, 995, 1356, 1764, 2166
Sau3AI GATC 6 cut(s) 126, 709, 1086, 1285, 1315, 1843
Sau96I GGNCC 2 cut(s) 31, 1495
SchI GAGTC 5 cut(s) 405, 1419, 1502, 1728, 1873
ScrFI CCNGG 6 cut(s) 786, 1170, 1218, 1740, 1812, 1964
SfaNI GCATC 4 cut(s) 771, 811, 1556, 2251
SfcI CTRYAG 3 cut(s) 651, 1599, 1660
SfoI GGCGCC 1 cut(s) 346
Sfr274I CTCGAG 1 cut(s) 1542
SlaI CTCGAG 1 cut(s) 1542
SmiMI CAYNNNNRTG 1 cut(s) 485
SmlI CTYRAG 5 cut(s) 620, 1067, 1442, 1542, 2320
SmoI CTYRAG 5 cut(s) 620, 1067, 1442, 1542, 2320
SpeI ACTAGT 1 cut(s) 2420
SphI GCATGC 1 cut(s) 1130
SsiI CCGC 3 cut(s) 648, 1716, 2415
SspDI GGCGCC 1 cut(s) 344
SspI AATATT 1 cut(s) 2185
SspMI CTAG 9 cut(s) 65, 180, 509, 1422, 1698, 1803, 1974, 2268, 2421
StyD4I CCNGG 6 cut(s) 784, 1168, 1216, 1738, 1810, 1962
StyI CCWWGG 4 cut(s) 27, 340, 1624, 1924
TaaI ACNGT 8 cut(s) 16, 981, 1212, 1387, 1792, 2155, 2243, 2401
TaiI ACGT 2 cut(s) 291, 406
TaqI TCGA 7 cut(s) 633, 708, 912, 1543, 1755, 2013, 2354
TatI WGTACW 5 cut(s) 467, 542, 812, 1207, 2217
TauI GCSGC 1 cut(s) 2417
TfiI GAWTC 4 cut(s) 248, 665, 722, 1428
Tru1I TTAA 9 cut(s) 174, 387, 419, 435, 840, 995, 1356, 1764, 2166
Tru9I TTAA 9 cut(s) 174, 387, 419, 435, 840, 995, 1356, 1764, 2166
TscAI CASTG 4 cut(s) 271, 1593, 1608, 2263
TseFI GTSAC 7 cut(s) 476, 641, 1049, 1212, 1730, 2119, 2401
TseI GCWGC 9 cut(s) 183, 332, 591, 1026, 1488, 1598, 1676, 1691, 1694
Tsp45I GTSAC 7 cut(s) 476, 641, 1049, 1212, 1730, 2119, 2401
TspDTI ATGAA 3 cut(s) 48, 630, 743
TspGWI ACGGA 2 cut(s) 1338, 1914
TspRI CASTG 4 cut(s) 271, 1593, 1608, 2263
XapI RAATTY 6 cut(s) 581, 1093, 1222, 1882, 2130, 2372
XceI RCATGY 4 cut(s) 278, 1130, 1657, 2218
XhoI CTCGAG 1 cut(s) 1542
XspI CTAG 9 cut(s) 65, 180, 509, 1422, 1698, 1803, 1974, 2268, 2421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.