Rorug06G0512300

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000881
Physical Location & Seq
Reverse (-)
62683554 .. 62688303
4750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0512300.1

Sequence Viewer

Length: 1446 bp
ATGAAGAATAAGGAAAATACAAGGCATGCTGCCAGAGTTGAAGAGCCTACTGTTCGAATCACACGAGCACGGGCTAAAGCCTTTGCCACATCAGCAGCAGAGGGCCTCTGCCTTGCCTCCTCCTCAAAATCTCTTCTTAAACAAGGCCAGAAGCGAGCTCTTCCAGCAAATTCCAAACACGGGGGGGCATCAGCTTCTCTAGTTTCCACCATCGGCTTGCAGCACAAAAGAAGGGCAGTGCTTAAAGATGTAACGAATTCAAATGTCGACTGGAGTGATGATGCATCTCAACTTCAGGCGAGAAGAGGGCCTGCAAAGAAGGTGAAGGTAGCCTCAAATCCAAGTGTTGCTGAACATTTTCCAATGGCTCAGGATGACATAAAAACAAAGTTAGCTGAGGAATTATCCAAAATAAGGGTGGCTGAACCACCAGAAAAGTTGCAAGTGAAAGAAGAGCCATTACCCCAGAGCATGTGTCATGCCTTGAGACAACATGGAGGAGCAGACCTAATACAGCATTCCATTAAATCTGCTCAACTTCAGAGGCCTCAAAAGAAAGAAGAATATATGGTCTGCGAGAGATTGGGATCCTCAAATGACTTGGACATCGTGGACATTGACGCAAACTTAAAAGATCCTCTAGCATGCAGCTTGTATGCCCCTGATATATACAGTAATATACGGCTTACAGAGATAGAGCGAAGACCATCAACTAATTATATGGAAACCTTGCAGCAAGATATCACTCCAAGCATGCGAGGAATTTTGATTGATTGGCTTGTGGAGGTTTCTGAAGAATATAAGCTGGTTCCTGATACACTTTACCTCACTGTGAATCTCATTGATCGCTTTCTCTCACAGAACTTTGTTGAAAAGCAAAGGCTCCAGCTGCTTGGAGTCACTTGCATGTTAATTGCCTCGAAGTATGAAGAAATTGTTGCACCACGAGTGGAAGAATTTTGTCTCATCACGGACAATACTTACTCAAGAGAAGAGGTATTAGAAATGGAGAGTGAAGTACTAAATTTCTTGCACTTCCAGTTATCTGTTCCAACCACGAAAACATTTCTTAGGAGGTTTGTTCAAGCGGCACAAGCTTCTTATAAGGTTCCTTGTGTTGAACTGGAGTTCTTGGCAAATTATTTAGCAGAGTTAACTATTGTTGAATATGGCTTCTTGAAGTTCCTACCTTCCGTCATCGCTGCCTCTGCTGTGTTCGTTGCCAGATGGACACTTAATCAGTCAGACCATCCATGGAACCCGACTTTAGAACACTATACCTCTTACAAAACATCAGAGCTCCACACTTCAGTTATTGCACTTGAAGATTTGCAATTGAACACCAAAGGCTGTTGCCTAAATGCTATACGTGAGAAGTATAGACACCAGAAGTTCAAATCCGTGGCTACTTTGATCTCTGCACAAAAGGTTGGTTCACTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

481

Amino Acids

54.21

Weight (kDa)

7.98

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclin_N PF00134 222 - 349 3.5e-48 Cyclin, N-terminal domain
Cyclin_C PF02984 351 - 471 2.3e-35 Cyclin, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1104
AccI GTMKAC 1 cut(s) 267
AciI CCGC 1 cut(s) 1088
AclWI GGATC 3 cut(s) 582, 595, 629
AcsI RAATTY 5 cut(s) 169, 256, 762, 956, 1024
AcuI CTGAAG 4 cut(s) 278, 524, 813, 1293
AfaI GTAC 1 cut(s) 1020
AfiI CCNNNNNNNGG 2 cut(s) 180, 414
AluBI AGCT 8 cut(s) 158, 194, 395, 651, 805, 889, 1097, 1300
AluI AGCT 8 cut(s) 158, 194, 395, 651, 805, 889, 1097, 1300
Alw21I GWGCWC 3 cut(s) 70, 160, 1302
Alw26I GTCTC 2 cut(s) 481, 968
AlwI GGATC 3 cut(s) 582, 595, 629
AoxI GGCC 4 cut(s) 103, 145, 308, 545
ApeKI GCWGC 7 cut(s) 29, 95, 220, 648, 733, 889, 1202
ApoI RAATTY 5 cut(s) 169, 256, 762, 956, 1024
Asp700I GAANNNNTTC 1 cut(s) 357
AspS9I GGNCC 2 cut(s) 103, 308
AsuHPI GGTGA 1 cut(s) 334
AsuII TTCGAA 1 cut(s) 55
BamHI GGATCC 1 cut(s) 587
BanII GRGCYC 2 cut(s) 160, 1302
BarI GAAGNNNNNNTAC 2 cut(s) 444, 476
BauI CACGAG 2 cut(s) 63, 945
BbsI GAAGAC 1 cut(s) 709
Bbv12I GWGCWC 3 cut(s) 70, 160, 1302
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 7 cut(s) 16, 107, 232, 660, 745, 876, 1189
BccI CCATC 4 cut(s) 218, 715, 1221, 1257
BceAI ACGGC 1 cut(s) 698
BcoDI GTCTC 2 cut(s) 481, 968
BfaI CTAG 2 cut(s) 200, 641
BisI GCNGC 8 cut(s) 30, 96, 221, 649, 734, 890, 1089, 1203
BlsI GCNGC 8 cut(s) 31, 97, 222, 650, 735, 891, 1090, 1204
BmcAI AGTACT 1 cut(s) 1020
BmgT120I GGNCC 2 cut(s) 103, 308
BmiI GGNNCC 5 cut(s) 589, 810, 884, 1110, 1259
BmsI GCATC 3 cut(s) 197, 271, 293
BpiI GAAGAC 1 cut(s) 709
BpmI CTGGAG 3 cut(s) 292, 869, 1145
Bpu10I CCTNAGC 2 cut(s) 369, 396
Bpu14I TTCGAA 1 cut(s) 55
BpuEI CTTGAG 2 cut(s) 505, 970
BsaAI YACGTR 1 cut(s) 1370
BsaBI GATNNNNATC 1 cut(s) 586
BsaJI CCNNGG 2 cut(s) 1253, 1401
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 414
Bse1I ACTGG 3 cut(s) 275, 1039, 1128
Bse8I GATNNNNATC 1 cut(s) 586
BseDI CCNNGG 2 cut(s) 1253, 1401
BseGI GGATG 2 cut(s) 379, 1249
BseJI GATNNNNATC 1 cut(s) 586
BseLI CCNNNNNNNGG 2 cut(s) 180, 414
BseMII CTCAG 2 cut(s) 383, 387
BseNI ACTGG 3 cut(s) 275, 1039, 1128
BseRI GAGGAG 3 cut(s) 109, 112, 513
BseXI GCAGC 7 cut(s) 16, 107, 232, 660, 745, 876, 1189
BsgI GTGCAG 1 cut(s) 1404
BshFI GGCC 4 cut(s) 105, 147, 310, 547
BsiHKAI GWGCWC 3 cut(s) 70, 160, 1302
BslI CCNNNNNNNGG 2 cut(s) 180, 414
BsmAI GTCTC 2 cut(s) 481, 968
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 4 cut(s) 105, 147, 310, 547
Bsp119I TTCGAA 1 cut(s) 55
Bsp1286I GDGCHC 3 cut(s) 70, 160, 1302
Bsp143I GATC 4 cut(s) 587, 634, 844, 1413
Bsp19I CCATGG 1 cut(s) 1253
BspACI CCGC 1 cut(s) 1088
BspANI GGCC 4 cut(s) 105, 147, 310, 547
BspCNI CTCAG 2 cut(s) 382, 388
BspLI GGNNCC 5 cut(s) 589, 810, 884, 1110, 1259
BspPI GGATC 3 cut(s) 582, 595, 629
BspQI GCTCTTC 3 cut(s) 36, 165, 447
BspT104I TTCGAA 1 cut(s) 55
BsrI ACTGG 3 cut(s) 275, 1039, 1128
BssECI CCNNGG 2 cut(s) 1253, 1401
BssMI GATC 4 cut(s) 587, 634, 844, 1413
BssSI CACGAG 2 cut(s) 63, 945
BssT1I CCWWGG 1 cut(s) 1253
Bst2BI CACGAG 2 cut(s) 63, 945
Bst4CI ACNGT 3 cut(s) 52, 674, 832
Bst6I CTCTTC 6 cut(s) 36, 138, 165, 298, 447, 987
BstBAI YACGTR 1 cut(s) 1370
BstBI TTCGAA 1 cut(s) 55
BstC8I GCNNGC 6 cut(s) 27, 156, 218, 312, 646, 755
BstDEI CTNAG 3 cut(s) 369, 396, 1070
BstDSI CCRYGG 2 cut(s) 1253, 1401
BstF5I GGATG 2 cut(s) 379, 1249
BstKTI GATC 4 cut(s) 590, 637, 847, 1416
BstMAI GTCTC 2 cut(s) 481, 968
BstMBI GATC 4 cut(s) 587, 634, 844, 1413
BstMWI GCNNNNNNNGC 5 cut(s) 92, 164, 889, 1094, 1208
BstNSI RCATGY 5 cut(s) 29, 475, 648, 757, 910
BstV1I GCAGC 7 cut(s) 16, 107, 232, 660, 745, 876, 1189
BstV2I GAAGAC 1 cut(s) 709
BstX2I RGATCY 2 cut(s) 587, 634
BstXI CCANNNNNNTGG 1 cut(s) 893
BstYI RGATCY 2 cut(s) 587, 634
BsuRI GGCC 4 cut(s) 105, 147, 310, 547
BtgI CCRYGG 2 cut(s) 1253, 1401
BtgZI GCGATG 1 cut(s) 1183
BtsCI GGATG 2 cut(s) 379, 1249
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 2 cut(s) 243, 828
Cac8I GCNNGC 6 cut(s) 27, 156, 218, 312, 646, 755
Cfr13I GGNCC 2 cut(s) 103, 308
CseI GACGC 1 cut(s) 629
Csp6I GTAC 1 cut(s) 1019
CviAII CATG 8 cut(s) 26, 472, 479, 494, 645, 754, 907, 1254
CviQI GTAC 1 cut(s) 1019
DdeI CTNAG 3 cut(s) 369, 396, 1070
DpnI GATC 4 cut(s) 589, 636, 846, 1415
DpnII GATC 4 cut(s) 587, 634, 844, 1413
Eam1104I CTCTTC 6 cut(s) 36, 138, 165, 298, 447, 987
EarI CTCTTC 6 cut(s) 36, 138, 165, 298, 447, 987
Ecl136II GAGCTC 2 cut(s) 158, 1300
Eco130I CCWWGG 1 cut(s) 1253
Eco147I AGGCCT 1 cut(s) 547
Eco24I GRGCYC 2 cut(s) 160, 1302
Eco32I GATATC 1 cut(s) 742
Eco53kI GAGCTC 2 cut(s) 158, 1300
Eco57I CTGAAG 4 cut(s) 278, 524, 813, 1293
EcoICRI GAGCTC 2 cut(s) 158, 1300
EcoO109I RGGNCCY 2 cut(s) 103, 308
EcoRI GAATTC 1 cut(s) 256
EcoRV GATATC 1 cut(s) 742
EcoT14I CCWWGG 1 cut(s) 1253
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 2 cut(s) 160, 1302
ErhI CCWWGG 1 cut(s) 1253
FaeI CATG 8 cut(s) 29, 475, 482, 497, 648, 757, 910, 1257
FatI CATG 8 cut(s) 25, 471, 478, 493, 644, 753, 906, 1253
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 8 cut(s) 30, 96, 221, 649, 734, 890, 1089, 1203
FokI GGATG 2 cut(s) 386, 1236
FriOI GRGCYC 2 cut(s) 160, 1302
Fsp4HI GCNGC 8 cut(s) 30, 96, 221, 649, 734, 890, 1089, 1203
FspBI CTAG 2 cut(s) 200, 641
GluI GCNGC 8 cut(s) 30, 96, 221, 649, 734, 890, 1089, 1203
GsuI CTGGAG 3 cut(s) 292, 869, 1145
HaeIII GGCC 4 cut(s) 105, 147, 310, 547
HgaI GACGC 1 cut(s) 629
Hin1II CATG 8 cut(s) 29, 475, 482, 497, 648, 757, 910, 1257
HincII GTYRAC 2 cut(s) 268, 1155
HindII GTYRAC 2 cut(s) 268, 1155
HindIII AAGCTT 1 cut(s) 1095
HinfI GANTC 3 cut(s) 57, 835, 897
HpaI GTTAAC 1 cut(s) 1155
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 4 cut(s) 268, 613, 1155, 1436
Hpy188I TCNGA 5 cut(s) 543, 793, 1246, 1297, 1445
Hpy188III TCNNGA 4 cut(s) 371, 812, 987, 1177
Hpy8I GTNNAC 4 cut(s) 268, 613, 1155, 1436
HpyAV CCTTC 4 cut(s) 225, 313, 319, 1200
HpyCH4III ACNGT 3 cut(s) 52, 674, 832
HpyCH4IV ACGT 1 cut(s) 1369
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 164, 889, 1094, 1208
HpyF3I CTNAG 3 cut(s) 369, 396, 1070
HpySE526I ACGT 1 cut(s) 1369
Hsp92II CATG 8 cut(s) 29, 475, 482, 497, 648, 757, 910, 1257
KspAI GTTAAC 1 cut(s) 1155
Kzo9I GATC 4 cut(s) 587, 634, 844, 1413
LguI GCTCTTC 3 cut(s) 36, 165, 447
LmnI GCTCC 3 cut(s) 500, 888, 1305
Lsp1109I GCAGC 7 cut(s) 16, 107, 232, 660, 745, 876, 1189
LweI GCATC 3 cut(s) 197, 271, 293
MaeI CTAG 2 cut(s) 200, 641
MaeII ACGT 1 cut(s) 1369
MaeIII GTNAC 2 cut(s) 250, 898
MalI GATC 4 cut(s) 589, 636, 846, 1415
MboI GATC 4 cut(s) 587, 634, 844, 1413
MfeI CAATTG 1 cut(s) 1334
MflI RGATCY 2 cut(s) 587, 634
MhlI GDGCHC 3 cut(s) 70, 160, 1302
MlyI GAGTC 1 cut(s) 906
MmeI TCCRAC 1 cut(s) 1076
Mph1103I ATGCAT 1 cut(s) 286
MroXI GAANNNNTTC 1 cut(s) 357
MseI TTAA 7 cut(s) 138, 243, 525, 629, 911, 1154, 1236
MslI CAYNNNNRTG 1 cut(s) 905
MspA1I CMGCKG 1 cut(s) 889
MunI CAATTG 1 cut(s) 1334
Mva1269I GAATGC 1 cut(s) 517
MwoI GCNNNNNNNGC 5 cut(s) 92, 164, 889, 1094, 1208
NcoI CCATGG 1 cut(s) 1253
NdeII GATC 4 cut(s) 587, 634, 844, 1413
NlaIII CATG 8 cut(s) 29, 475, 482, 497, 648, 757, 910, 1257
NlaIV GGNNCC 5 cut(s) 589, 810, 884, 1110, 1259
NmuCI GTSAC 1 cut(s) 898
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 5 cut(s) 29, 475, 648, 757, 910
NspV TTCGAA 1 cut(s) 55
PaeI GCATGC 3 cut(s) 29, 648, 757
PceI AGGCCT 1 cut(s) 547
PciSI GCTCTTC 3 cut(s) 36, 165, 447
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PctI GAATGC 1 cut(s) 517
PdmI GAANNNNTTC 1 cut(s) 357
PfeI GAWTC 2 cut(s) 57, 835
PkrI GCNGC 8 cut(s) 31, 97, 222, 650, 735, 891, 1090, 1204
PleI GAGTC 1 cut(s) 905
PpsI GAGTC 1 cut(s) 905
Ppu21I YACGTR 1 cut(s) 1370
PsiI TTATAA 1 cut(s) 1104
Psp124BI GAGCTC 2 cut(s) 160, 1302
PspN4I GGNNCC 5 cut(s) 589, 810, 884, 1110, 1259
PspPI GGNCC 2 cut(s) 103, 308
PsuI RGATCY 2 cut(s) 587, 634
PvuII CAGCTG 1 cut(s) 889
RsaI GTAC 1 cut(s) 1020
RsaNI GTAC 1 cut(s) 1019
RseI CAYNNNNRTG 1 cut(s) 905
SacI GAGCTC 2 cut(s) 160, 1302
SalI GTCGAC 1 cut(s) 266
SapI GCTCTTC 3 cut(s) 36, 165, 447
SaqAI TTAA 7 cut(s) 138, 243, 525, 629, 911, 1154, 1236
SatI GCNGC 8 cut(s) 30, 96, 221, 649, 734, 890, 1089, 1203
Sau3AI GATC 4 cut(s) 587, 634, 844, 1413
Sau96I GGNCC 2 cut(s) 103, 308
ScaI AGTACT 1 cut(s) 1020
SchI GAGTC 1 cut(s) 906
SduI GDGCHC 3 cut(s) 70, 160, 1302
SfaNI GCATC 3 cut(s) 197, 271, 293
SfuI TTCGAA 1 cut(s) 55
SmiMI CAYNNNNRTG 1 cut(s) 905
SmlI CTYRAG 2 cut(s) 484, 985
SmoI CTYRAG 2 cut(s) 484, 985
SphI GCATGC 3 cut(s) 29, 648, 757
SseBI AGGCCT 1 cut(s) 547
SsiI CCGC 1 cut(s) 1088
SspMI CTAG 2 cut(s) 200, 641
SstI GAGCTC 2 cut(s) 160, 1302
StuI AGGCCT 1 cut(s) 547
StyI CCWWGG 1 cut(s) 1253
TaaI ACNGT 3 cut(s) 52, 674, 832
TaiI ACGT 1 cut(s) 1372
TaqI TCGA 3 cut(s) 55, 267, 920
TatI WGTACW 1 cut(s) 1018
TauI GCSGC 1 cut(s) 1091
TfiI GAWTC 2 cut(s) 57, 835
Tru1I TTAA 7 cut(s) 138, 243, 525, 629, 911, 1154, 1236
Tru9I TTAA 7 cut(s) 138, 243, 525, 629, 911, 1154, 1236
TscAI CASTG 2 cut(s) 243, 835
TseFI GTSAC 1 cut(s) 898
TseI GCWGC 7 cut(s) 29, 95, 220, 648, 733, 889, 1202
Tsp45I GTSAC 1 cut(s) 898
TspDTI ATGAA 2 cut(s) 17, 942
TspGWI ACGGA 3 cut(s) 986, 1183, 1390
TspRI CASTG 2 cut(s) 243, 835
XapI RAATTY 5 cut(s) 169, 256, 762, 956, 1024
XceI RCATGY 5 cut(s) 29, 475, 648, 757, 910
XcmI CCANNNNNNNNNTGG 1 cut(s) 415
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 357
XspI CTAG 2 cut(s) 200, 641
ZrmI AGTACT 1 cut(s) 1020
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.