Rmu_ssc0000421.1_g000033

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000421.1
OG0000881
Physical Location & Seq
Reverse (-)
133365 .. 135573
2209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000421.1_g000033.1.cds

Sequence Viewer

Length: 1362 bp
atgtctatgacctctaccgagccaagcagcagagatggttgcttcatggtccgtgacaatgcccttctgacacgtgtcccggccaacatcatcgtcactccggccactgataaatcagctttcatcggagccacctctgaagaccgtagctctcgccatgttttccctctcggacttcttgagggatacaagtttatgtgcctgttcagattcaaaatctggtggatgataccgagttttggggattctggatgtgatgttccggcagagactcaaatgcttctcttggaagcaacgcaaaagtcgagtagtagtagtattcccgacaaaaatgggttctcatctgagtctgaggagccaacaagtgctgagaacaaaagcactttctacattctgctattgccagtcttggatggagcattcagggctactttgcaaggcaacactgttaatgagcttgagttttgcgttgaaagcggggaccgtgaagttcaaacctcacgagtaactgaagcagtatttgtgaattcaggagatgacccatataagctcattagggactctattaagattcttgccaagcacaaaggtacattcaatcatttagaagacaagaagataccagcacatgtagactggtttggatggaacacatgggatgcattttataatgacgttgatccggaaggaatcgagcttggactcaaaagtctttccgaaggagggtgcccccccgaaatgtttgtagttttgtacatcgatgaaggatggtttgtggccaggttggcaagcttagaagaaaatgataggttcaagggctacccctcgggaaagtcatcatttcctaatctccgcgagtttgtgaaaaccatcaaagaggaacatggactgaagttagtatatgcatggcacgctttacttggttcatggggagggctacttccaacatctgaagcactgaggaaatacaatccccaaatcaaatccatagttcagtctcctggtaacaagagtaacgtcatatgtggaaccctcgatccaatggagaaacatgggatcggaatgattgacccttcgatgatttatagattttacgatgatctacatagctaccttgcaagttgcaatatagacggagtgaaggtggatatacacaatgaagtggaactccttgcttctggttatggaggccgcgttgcattgatgaggcatttccaagaagccctcgaggaatctgtcatgagaaactttggcagcaataaccttctttgttccatgagtctaagcaatgactacatttacaggttaaacttagctagattttttccattagccaacaattggagatcagattttaattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

453

Amino Acids

50.83

Weight (kDa)

5.21

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AasI GACNNNNNNGTC 1 cut(s) 708
AbsI CCTCGAGG 1 cut(s) 1226
AccB1I GGYRCC 1 cut(s) 726
AccB7I CCANNNNNTGG 1 cut(s) 1341
AccI GTMKAC 1 cut(s) 633
AccII CGCG 2 cut(s) 855, 1194
AccIII TCCGGA 1 cut(s) 682
AciI CCGC 3 cut(s) 477, 853, 1192
AclWI GGATC 3 cut(s) 674, 1031, 1064
AcoI YGGCCR 3 cut(s) 81, 102, 777
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 4 cut(s) 159, 531, 911, 972
AcvI CACGTG 1 cut(s) 74
AfaI GTAC 2 cut(s) 592, 755
AfiI CCNNNNNNNGG 3 cut(s) 239, 723, 1341
AflIII ACRYGT 3 cut(s) 71, 73, 628
AgsI TTSAA 5 cut(s) 214, 473, 494, 598, 814
AjnI CCWGG 2 cut(s) 779, 1000
AloI GAACNNNNNNTCC 4 cut(s) 243, 275, 1021, 1053
AluBI AGCT 8 cut(s) 119, 150, 457, 550, 697, 792, 1110, 1316
AluI AGCT 8 cut(s) 119, 150, 457, 550, 697, 792, 1110, 1316
Alw26I GTCTC 2 cut(s) 263, 1002
AlwI GGATC 3 cut(s) 674, 1031, 1064
Ama87I CYCGRG 2 cut(s) 826, 1226
Aor13HI TCCGGA 1 cut(s) 682
AoxI GGCC 4 cut(s) 81, 102, 777, 1189
ApeKI GCWGC 2 cut(s) 27, 1254
ApoI RAATTY 1 cut(s) 526
AspS9I GGNCC 2 cut(s) 49, 481
AsuC2I CCSGG 1 cut(s) 80
AvaI CYCGRG 2 cut(s) 826, 1226
AvaII GGWCC 2 cut(s) 49, 481
BaeGI GKGCMC 1 cut(s) 731
BalI TGGCCA 1 cut(s) 779
BanI GGYRCC 1 cut(s) 726
BauI CACGAG 1 cut(s) 501
BbrPI CACGTG 1 cut(s) 74
BbsI GAAGAC 2 cut(s) 147, 615
BbvI GCAGC 2 cut(s) 39, 1266
BccI CCATC 5 cut(s) 29, 407, 639, 762, 878
BciT130I CCWGG 2 cut(s) 781, 1002
BciVI GTATCC 1 cut(s) 179
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 2 cut(s) 263, 1002
BfaI CTAG 1 cut(s) 1317
BfuI GTATCC 1 cut(s) 179
BglI GCCNNNNNGGC 1 cut(s) 785
BisI GCNGC 3 cut(s) 28, 1192, 1255
BlsI GCNGC 3 cut(s) 29, 1193, 1256
Bme1390I CCNGG 3 cut(s) 80, 781, 1002
Bme18I GGWCC 2 cut(s) 49, 481
BmeT110I CYCGRG 2 cut(s) 826, 1226
BmgT120I GGNCC 2 cut(s) 49, 481
BmiI GGNNCC 5 cut(s) 130, 357, 482, 728, 1030
BmrFI CCNGG 3 cut(s) 80, 781, 1002
BmsI GCATC 1 cut(s) 649
BoxI GACNNNNGTC 1 cut(s) 74
BpiI GAAGAC 2 cut(s) 147, 615
BpuEI CTTGAG 2 cut(s) 200, 479
BpuMI CCSGG 1 cut(s) 80
Bsa29I ATCGAT 1 cut(s) 759
BsaAI YACGTR 1 cut(s) 74
BsaJI CCNNGG 1 cut(s) 825
BsaWI WCCGGW 1 cut(s) 682
BsaXI ACNNNNNCTCC 4 cut(s) 1128, 1158, 1179, 1209
Bsc4I CCNNNNNNNGG 3 cut(s) 239, 723, 1341
Bse1I ACTGG 2 cut(s) 404, 641
Bse3DI GCAATG 1 cut(s) 1294
BseAI TCCGGA 1 cut(s) 682
BseBI CCWGG 2 cut(s) 781, 1002
BseCI ATCGAT 1 cut(s) 759
BseDI CCNNGG 1 cut(s) 825
BseGI GGATG 6 cut(s) 231, 257, 418, 650, 664, 773
BseLI CCNNNNNNNGG 3 cut(s) 239, 723, 1341
BseMI GCAATG 1 cut(s) 1294
BseMII CTCAG 4 cut(s) 336, 342, 360, 950
BseNI ACTGG 2 cut(s) 404, 641
BseRI GAGGAG 1 cut(s) 368
BseSI GKGCMC 1 cut(s) 731
BseXI GCAGC 2 cut(s) 39, 1266
Bsh1236I CGCG 2 cut(s) 855, 1194
BshFI GGCC 4 cut(s) 83, 104, 779, 1191
BshNI GGYRCC 1 cut(s) 726
BshVI ATCGAT 1 cut(s) 759
BsiHKCI CYCGRG 2 cut(s) 826, 1226
BsiSI CCGG 4 cut(s) 80, 101, 263, 683
BslFI GGGAC 3 cut(s) 62, 494, 572
BslI CCNNNNNNNGG 3 cut(s) 239, 723, 1341
BsmAI GTCTC 2 cut(s) 263, 1002
BsmFI GGGAC 3 cut(s) 62, 494, 572
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 4 cut(s) 83, 104, 779, 1191
BsoBI CYCGRG 2 cut(s) 826, 1226
Bsp1286I GDGCHC 1 cut(s) 731
Bsp13I TCCGGA 1 cut(s) 682
Bsp1407I TGTACA 1 cut(s) 753
Bsp143I GATC 5 cut(s) 679, 1036, 1056, 1099, 1346
BspACI CCGC 3 cut(s) 477, 853, 1192
BspANI GGCC 4 cut(s) 83, 104, 779, 1191
BspCNI CTCAG 4 cut(s) 337, 343, 361, 951
BspDI ATCGAT 1 cut(s) 759
BspEI TCCGGA 1 cut(s) 682
BspFNI CGCG 2 cut(s) 855, 1194
BspHI TCATGA 1 cut(s) 1239
BspLI GGNNCC 5 cut(s) 130, 357, 482, 728, 1030
BspPI GGATC 3 cut(s) 674, 1031, 1064
BspT107I GGYRCC 1 cut(s) 726
BsrDI GCAATG 1 cut(s) 1294
BsrGI TGTACA 1 cut(s) 753
BsrI ACTGG 2 cut(s) 404, 641
BssECI CCNNGG 1 cut(s) 825
BssMI GATC 5 cut(s) 679, 1036, 1056, 1099, 1346
BssSI CACGAG 1 cut(s) 501
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 2 cut(s) 781, 1002
Bst4CI ACNGT 3 cut(s) 146, 448, 485
BstAUI TGTACA 1 cut(s) 753
BstBAI YACGTR 1 cut(s) 74
BstC8I GCNNGC 2 cut(s) 790, 912
BstDEI CTNAG 7 cut(s) 345, 351, 369, 793, 959, 1283, 1312
BstF5I GGATG 6 cut(s) 231, 257, 418, 650, 664, 773
BstFNI CGCG 2 cut(s) 855, 1194
BstKTI GATC 5 cut(s) 682, 1039, 1059, 1102, 1349
BstMAI GTCTC 2 cut(s) 263, 1002
BstMBI GATC 5 cut(s) 679, 1036, 1056, 1099, 1346
BstMWI GCNNNNNNNGC 4 cut(s) 425, 474, 785, 911
BstNI CCWGG 2 cut(s) 781, 1002
BstNSI RCATGY 1 cut(s) 632
BstPAI GACNNNNGTC 1 cut(s) 74
BstSCI CCNGG 3 cut(s) 78, 779, 1000
BstSLI GKGCMC 1 cut(s) 731
BstUI CGCG 2 cut(s) 855, 1194
BstV1I GCAGC 2 cut(s) 39, 1266
BstV2I GAAGAC 2 cut(s) 147, 615
Bsu15I ATCGAT 1 cut(s) 759
BsuI GTATCC 1 cut(s) 179
BsuRI GGCC 4 cut(s) 83, 104, 779, 1191
BsuTUI ATCGAT 1 cut(s) 759
BtsCI GGATG 6 cut(s) 231, 257, 418, 650, 664, 773
BtsIMutI CAGTG 3 cut(s) 105, 444, 956
Cac8I GCNNGC 2 cut(s) 790, 912
CciI TCATGA 1 cut(s) 1239
Cfr13I GGNCC 2 cut(s) 49, 481
ClaI ATCGAT 1 cut(s) 759
Csp6I GTAC 2 cut(s) 591, 754
CviQI GTAC 2 cut(s) 591, 754
DdeI CTNAG 7 cut(s) 345, 351, 369, 793, 959, 1283, 1312
DpnI GATC 5 cut(s) 681, 1038, 1058, 1101, 1348
DpnII GATC 5 cut(s) 679, 1036, 1056, 1099, 1346
DrdI GACNNNNNNGTC 1 cut(s) 708
DseDI GACNNNNNNGTC 1 cut(s) 708
EaeI YGGCCR 3 cut(s) 81, 102, 777
Eco47I GGWCC 2 cut(s) 49, 481
Eco57I CTGAAG 4 cut(s) 159, 531, 911, 972
Eco72I CACGTG 1 cut(s) 74
Eco88I CYCGRG 2 cut(s) 826, 1226
EcoRI GAATTC 1 cut(s) 526
EcoRII CCWGG 2 cut(s) 779, 1000
EcoT22I ATGCAT 2 cut(s) 664, 907
FaqI GGGAC 3 cut(s) 62, 494, 572
FauI CCCGC 1 cut(s) 470
FauNDI CATATG 1 cut(s) 1022
FblI GTMKAC 1 cut(s) 633
Fnu4HI GCNGC 3 cut(s) 28, 1192, 1255
FokI GGATG 6 cut(s) 238, 264, 425, 657, 671, 780
Fsp4HI GCNGC 3 cut(s) 28, 1192, 1255
FspBI CTAG 1 cut(s) 1317
GluI GCNGC 3 cut(s) 28, 1192, 1255
HaeIII GGCC 4 cut(s) 83, 104, 779, 1191
HapII CCGG 4 cut(s) 80, 101, 263, 683
HindIII AAGCTT 1 cut(s) 790
HpaII CCGG 4 cut(s) 80, 101, 263, 683
Hpy166II GTNNAC 1 cut(s) 634
Hpy188III TCNNGA 8 cut(s) 179, 249, 323, 501, 531, 683, 828, 1240
Hpy8I GTNNAC 1 cut(s) 634
HpyAV CCTTC 7 cut(s) 74, 680, 713, 758, 1083, 1135, 1274
HpyCH4III ACNGT 3 cut(s) 146, 448, 485
HpyCH4IV ACGT 3 cut(s) 73, 675, 1017
HpyCH4V TGCA 6 cut(s) 436, 662, 905, 1118, 1125, 1199
HpyF10VI GCNNNNNNNGC 4 cut(s) 425, 474, 785, 911
HpyF3I CTNAG 7 cut(s) 345, 351, 369, 793, 959, 1283, 1312
HpySE526I ACGT 3 cut(s) 73, 675, 1017
Kpn2I TCCGGA 1 cut(s) 682
Kzo9I GATC 5 cut(s) 679, 1036, 1056, 1099, 1346
LmnI GCTCC 3 cut(s) 128, 355, 416
Lsp1109I GCAGC 2 cut(s) 39, 1266
LweI GCATC 1 cut(s) 649
MaeI CTAG 1 cut(s) 1317
MaeII ACGT 3 cut(s) 73, 675, 1017
MaeIII GTNAC 5 cut(s) 53, 94, 505, 1004, 1013
MalI GATC 5 cut(s) 681, 1038, 1058, 1101, 1348
MboI GATC 5 cut(s) 679, 1036, 1056, 1099, 1346
MboII GAAGA 4 cut(s) 152, 620, 628, 809
MfeI CAATTG 1 cut(s) 1339
MhlI GDGCHC 1 cut(s) 731
MlsI TGGCCA 1 cut(s) 779
MluCI AATT 3 cut(s) 526, 1339, 1357
MluNI TGGCCA 1 cut(s) 779
MlyI GAGTC 5 cut(s) 265, 356, 554, 696, 1288
MmeI TCCRAC 1 cut(s) 968
Mox20I TGGCCA 1 cut(s) 779
Mph1103I ATGCAT 2 cut(s) 664, 907
MroI TCCGGA 1 cut(s) 682
MscI TGGCCA 1 cut(s) 779
MseI TTAA 4 cut(s) 450, 567, 1307, 1356
Msp20I TGGCCA 1 cut(s) 779
MspI CCGG 4 cut(s) 80, 101, 263, 683
MspR9I CCNGG 3 cut(s) 80, 781, 1002
MunI CAATTG 1 cut(s) 1339
Mva1269I GAATGC 1 cut(s) 419
MvaI CCWGG 2 cut(s) 781, 1002
MvnI CGCG 2 cut(s) 855, 1194
MwoI GCNNNNNNNGC 4 cut(s) 425, 474, 785, 911
NciI CCSGG 1 cut(s) 80
NdeI CATATG 1 cut(s) 1022
NdeII GATC 5 cut(s) 679, 1036, 1056, 1099, 1346
NlaIV GGNNCC 5 cut(s) 130, 357, 482, 728, 1030
NmuCI GTSAC 2 cut(s) 53, 94
NsiI ATGCAT 2 cut(s) 664, 907
NspI RCATGY 1 cut(s) 632
PaeR7I CTCGAG 1 cut(s) 1226
PagI TCATGA 1 cut(s) 1239
PciI ACATGT 1 cut(s) 628
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PctI GAATGC 1 cut(s) 419
PfeI GAWTC 5 cut(s) 210, 245, 571, 690, 1232
PflMI CCANNNNNTGG 1 cut(s) 1341
PkrI GCNGC 3 cut(s) 29, 1193, 1256
PleI GAGTC 5 cut(s) 265, 355, 554, 696, 1287
PmaCI CACGTG 1 cut(s) 74
PmlI CACGTG 1 cut(s) 74
PpsI GAGTC 5 cut(s) 265, 355, 554, 696, 1287
Ppu21I YACGTR 1 cut(s) 74
PscI ACATGT 1 cut(s) 628
PshAI GACNNNNGTC 1 cut(s) 74
PsiI TTATAA 1 cut(s) 669
Psp6I CCWGG 2 cut(s) 779, 1000
PspCI CACGTG 1 cut(s) 74
PspGI CCWGG 2 cut(s) 779, 1000
PspN4I GGNNCC 5 cut(s) 130, 357, 482, 728, 1030
PspPI GGNCC 2 cut(s) 49, 481
PspXI VCTCGAGB 1 cut(s) 1226
RsaI GTAC 2 cut(s) 592, 755
RsaNI GTAC 2 cut(s) 591, 754
SaqAI TTAA 4 cut(s) 450, 567, 1307, 1356
SatI GCNGC 3 cut(s) 28, 1192, 1255
Sau3AI GATC 5 cut(s) 679, 1036, 1056, 1099, 1346
Sau96I GGNCC 2 cut(s) 49, 481
SchI GAGTC 5 cut(s) 265, 356, 554, 696, 1288
ScrFI CCNGG 3 cut(s) 80, 781, 1002
SduI GDGCHC 1 cut(s) 731
SfaNI GCATC 1 cut(s) 649
Sfr274I CTCGAG 1 cut(s) 1226
SinI GGWCC 2 cut(s) 49, 481
SlaI CTCGAG 1 cut(s) 1226
SmlI CTYRAG 3 cut(s) 179, 458, 1226
SmoI CTYRAG 3 cut(s) 179, 458, 1226
Sse9I AATT 3 cut(s) 526, 1339, 1357
SsiI CCGC 3 cut(s) 477, 853, 1192
SspMI CTAG 1 cut(s) 1317
StyD4I CCNGG 3 cut(s) 78, 779, 1000
TaaI ACNGT 3 cut(s) 146, 448, 485
TaiI ACGT 3 cut(s) 76, 678, 1020
TaqI TCGA 6 cut(s) 305, 693, 759, 1035, 1076, 1227
TasI AATT 3 cut(s) 526, 1339, 1357
TatI WGTACW 1 cut(s) 753
TauI GCSGC 1 cut(s) 1194
TfiI GAWTC 5 cut(s) 210, 245, 571, 690, 1232
Tru1I TTAA 4 cut(s) 450, 567, 1307, 1356
Tru9I TTAA 4 cut(s) 450, 567, 1307, 1356
TscAI CASTG 3 cut(s) 112, 451, 963
TseFI GTSAC 2 cut(s) 53, 94
TseI GCWGC 2 cut(s) 27, 1254
Tsp45I GTSAC 2 cut(s) 53, 94
TspDTI ATGAA 5 cut(s) 34, 112, 777, 915, 1173
TspGWI ACGGA 2 cut(s) 41, 1149
TspRI CASTG 3 cut(s) 112, 451, 963
Van91I CCANNNNNTGG 1 cut(s) 1341
VpaK11BI GGWCC 2 cut(s) 49, 481
XapI RAATTY 1 cut(s) 526
XceI RCATGY 1 cut(s) 632
XhoI CTCGAG 1 cut(s) 1226
XmiI GTMKAC 1 cut(s) 633
XspI CTAG 1 cut(s) 1317
Zsp2I ATGCAT 2 cut(s) 664, 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.