Rh7BG124300

Galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000881
Physical Location & Seq
Reverse (-)
9239211 .. 9249648
10438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG124300.1

Sequence Viewer

Length: 2286 bp
ATGACTATGACCTCTACCGAGCCAAGCAGCAGAGATGGTTGCTTCATGGTCCGTGACAATGCCCTTCTGACACGTGTCCCGGCCAACATCACCGTCACTCCGGCCACTGGTGAATCAGCTTTCATCGGAGCCACCTCTGAAAACCGTAGCTCTCGCCATGTCTTCTCCCTCGGACTTCTTGAGGGATACAAGTTTATGTGCCTGTTCAGATTCAAAATCTGGTGGATGATACCGAGTTTTGGGGATTCTGGATGTGATGTTCCGGCAGAGACTCAAATGCTTCTCTTGGAAGCAACGCAAACGTCGAGAAGTAGTAGTATTCCCGACAAAAATGGGTTCTCATCTGAGTCTGAGGAGCCAACAAGTGCTGAGAACAAAAGCACTTTCTACATTCTGCTATTGCCAGTCTTGGATGGAGCATTCAGGGCTACTTTGCAAGGCAACACTGTTAATGAGCTTGAGTTTTGCGTTGAAAGCGGGGACCGTGAAGTTCAAACCTCACGAGTAACTGAAGCAGTATTTGTGAATTCAGGAGATGACCCATATAAGCTCATTAGGGACTCTATTAAGATTCTTGCCAAGCACAAAGGTACATTCAATCATTTAGAAGACAAGAAGATACCAGCACATGTAGACTGGTTTGGATGGAACACATGGGATGCATTTTATAATGACGTTGATCCGGAAGGAATCGTGCTTGGACTCAAAAGTCTTTCCGAAGGAGGGTGCCCCCCGAAATGTTTGTACATCGATGAAGGATGGCAGACTAAAGTAATTGAAAATGAAACTGAACTACCAAATACAGCGAAGTTTGTGGCCAGGTTGGCAAGCTTAGAAGAAAATGATAGGTTCAAGGGCTACCCCTCGGGAAAGTCATCATTTCCTAATCTCCGCGAGTTTGTGAAAACCATCAAAGAGGAACATGGACTGAAGTTAGTATATGCATGGCACGCTTTACTTGGTTCATGGGGAGGGCTACTTCCAACATCTGAAGCACTGAGGAAATACAATCCCCAAATCAAATCCATACTTCAGTCTCCTGGTAACAAGAGTAACGTCATATGTGGAACCCTAGATCCAATGGAGAAACATGGGATCGGAATGATTGACCCTTCGATGATTTATAGATTTTATGATGATCTACATAGCTACCTTGCAAGTTGCAATATAGACGGAGTGAAGGTGGATATACACAATGAAGTGGAACTCCTTGCTTCTGGTTATGGAGTCCGCGTTGCATTGATGAGGCATTTCCAAGAAGCCCTCGAGGAATCTGTCATGAGAAACTTTGGCAGCAATAACCTTATTTGTTCCATGAGTCTAAGCAATGACTACATTTACAGCTCAAAGAAGAGTGCAGCTTCAAGAGTGTCAGAGGACTTCATGCCACTAGAGAAAACATTTCAGACCCTACATGTTGCTGCAGTAGCTTTTAACAGTCTTCTTATGGGAGAGATTGTTGTACCAGATTGGGACATGTTCTTTAGCGACCACTACACCCGGGAGTTCCATGCTGCAGCCAGAGCATTGGGTGGATGTCCAGTATATGTAAGTGACAAGCCTGGCAGCCATAATTTCAATGTCCTCAAAAAGCTTGTACTGCCTGATGGCTCTATTCTAAGAGCTAGATTCGCTGGCCGGCCTACTAGGGACTGCTTATTCAGTGACCCTGTGGTCGATGGAAAAAGTCTGCTGAAGATTTGGAATCTAAACAAGGTATCAGGAGTTATTGGTGTTTTCAATTGCCAAAGAGCCGGAAAGTGGCCACCAATTGTAGGTGCTCAATATGTGCCTCCCTCTGAATCTGCGCCGCCTCTTATAGGCCTCGTCAGCCCCATAGATATTAATATGCTTGAAGATGTTGCAAATGAAAGTTGGAGAGGAGAATGTGCAGTATATGCATACCATTCAGGTACTCTTTCTGTAATGCCAAAGAAAGACCACTTTGAAGTATCCTTGGATGTGCTAGAATGTGAAGTTTTCACCATAACTCCTATTATGGTTTTTGGTGACAACCTTCAATTTGCCCCAATAGGGTTGCTTGATATGTACAATTCAGGAGGAGCACTTGAATCTCTAGATGTCAGCAACAATGATCTGTTCGACTGTGTTGTGAAGGTTCGAGTCAGAGGATGCTGTCGATTTGGAGCCTACTCAAACAAAAAACCAAAGTCTTGTCTGGTGGACAAGAAAGAGGAGTTTATAGTCTACAATGCTAATAATGGTCTGTTGGTACTTAAACTTCAAGGCGATTGCAAAGTAAAAGAGATTGAGTTCATGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

761

Amino Acids

84.61

Weight (kDa)

5.48

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 12 - 747 1.4e-240 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AasI GACNNNNNNGTC 2 cut(s) 708, 1673
AbsI CCTCGAGG 1 cut(s) 1265
AccB1I GGYRCC 1 cut(s) 726
AccI GTMKAC 2 cut(s) 633, 2208
AccII CGCG 2 cut(s) 894, 1233
AccIII TCCGGA 1 cut(s) 682
AciI CCGC 4 cut(s) 477, 892, 1231, 1811
AclWI GGATC 3 cut(s) 674, 1070, 1103
AcoI YGGCCR 5 cut(s) 81, 102, 816, 1636, 1763
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 5 cut(s) 531, 950, 1011, 1016, 1715
AcvI CACGTG 1 cut(s) 74
AfaI GTAC 8 cut(s) 592, 746, 1464, 1599, 1915, 2051, 2235, 2282
AfiI CCNNNNNNNGG 7 cut(s) 107, 239, 723, 1761, 1775, 1820, 2034
AflIII ACRYGT 5 cut(s) 71, 73, 628, 1414, 1476
AjnI CCWGG 3 cut(s) 818, 1039, 1561
AloI GAACNNNNNNTCC 4 cut(s) 243, 275, 1060, 1092
Alw21I GWGCWC 2 cut(s) 1783, 2068
Alw26I GTCTC 2 cut(s) 263, 1041
AlwI GGATC 3 cut(s) 674, 1070, 1103
Ama87I CYCGRG 3 cut(s) 865, 1265, 1500
Aor13HI TCCGGA 1 cut(s) 682
AoxI GGCC 7 cut(s) 81, 102, 816, 1636, 1640, 1763, 1822
ApeKI GCWGC 7 cut(s) 27, 1293, 1358, 1421, 1514, 1517, 1566
ApoI RAATTY 1 cut(s) 526
AseI ATTAAT 1 cut(s) 1845
AspLEI GCGC 1 cut(s) 1810
AspS9I GGNCC 2 cut(s) 49, 481
AsuC2I CCSGG 3 cut(s) 80, 1501, 1502
AsuHPI GGTGA 4 cut(s) 82, 122, 1975, 2021
AvaI CYCGRG 3 cut(s) 865, 1265, 1500
AvaII GGWCC 2 cut(s) 49, 481
BaeGI GKGCMC 1 cut(s) 731
BalI TGGCCA 2 cut(s) 818, 1765
BanI GGYRCC 1 cut(s) 726
BarI GAAGNNNNNNTAC 2 cut(s) 2226, 2258
BauI CACGAG 1 cut(s) 501
BbrPI CACGTG 1 cut(s) 74
BbsI GAAGAC 3 cut(s) 154, 615, 1433
Bbv12I GWGCWC 2 cut(s) 1783, 2068
BbvI GCAGC 7 cut(s) 39, 1305, 1370, 1408, 1501, 1529, 1578
BccI CCATC 7 cut(s) 29, 407, 639, 753, 917, 1601, 1673
BciT130I CCWGG 3 cut(s) 820, 1041, 1563
BciVI GTATCC 2 cut(s) 179, 1963
BcnI CCSGG 3 cut(s) 80, 1501, 1502
BcoDI GTCTC 2 cut(s) 263, 1041
BfaI CTAG 6 cut(s) 1073, 1391, 1626, 1647, 1967, 2078
BfmI CTRYAG 2 cut(s) 1422, 1515
BfuI GTATCC 2 cut(s) 179, 1963
BglI GCCNNNNNGGC 1 cut(s) 824
BisI GCNGC 8 cut(s) 28, 1294, 1359, 1422, 1515, 1518, 1567, 1811
BlsI GCNGC 8 cut(s) 29, 1295, 1360, 1423, 1516, 1519, 1568, 1812
Bme1390I CCNGG 6 cut(s) 80, 820, 1041, 1501, 1502, 1563
Bme18I GGWCC 2 cut(s) 49, 481
BmeT110I CYCGRG 3 cut(s) 865, 1265, 1500
BmgT120I GGNCC 2 cut(s) 49, 481
BmiI GGNNCC 6 cut(s) 130, 357, 482, 728, 1069, 2149
BmrFI CCNGG 6 cut(s) 80, 820, 1041, 1501, 1502, 1563
BmsI GCATC 2 cut(s) 649, 2123
BoxI GACNNNNGTC 1 cut(s) 74
BpiI GAAGAC 3 cut(s) 154, 615, 1433
BpuEI CTTGAG 2 cut(s) 200, 479
BpuMI CCSGG 3 cut(s) 80, 1501, 1502
Bsa29I ATCGAT 1 cut(s) 750
BsaAI YACGTR 1 cut(s) 74
BsaJI CCNNGG 4 cut(s) 169, 864, 1500, 1956
BsaWI WCCGGW 1 cut(s) 682
Bsc4I CCNNNNNNNGG 7 cut(s) 107, 239, 723, 1761, 1775, 1820, 2034
Bse118I RCCGGY 1 cut(s) 1638
Bse1I ACTGG 4 cut(s) 112, 404, 641, 1541
Bse3DI GCAATG 1 cut(s) 1333
BseAI TCCGGA 1 cut(s) 682
BseBI CCWGG 3 cut(s) 820, 1041, 1563
BseCI ATCGAT 1 cut(s) 750
BseDI CCNNGG 4 cut(s) 169, 864, 1500, 1956
BseGI GGATG 9 cut(s) 231, 257, 418, 650, 664, 764, 1541, 1966, 2138
BseLI CCNNNNNNNGG 7 cut(s) 107, 239, 723, 1761, 1775, 1820, 2034
BseMI GCAATG 1 cut(s) 1333
BseMII CTCAG 4 cut(s) 336, 342, 360, 989
BseNI ACTGG 4 cut(s) 112, 404, 641, 1541
BseRI GAGGAG 4 cut(s) 368, 1896, 2076, 2210
BseSI GKGCMC 1 cut(s) 731
BseXI GCAGC 7 cut(s) 39, 1305, 1370, 1408, 1501, 1529, 1578
BsgI GTGCAG 2 cut(s) 1377, 1911
Bsh1236I CGCG 2 cut(s) 894, 1233
BshFI GGCC 7 cut(s) 83, 104, 818, 1638, 1642, 1765, 1824
BshNI GGYRCC 1 cut(s) 726
BshVI ATCGAT 1 cut(s) 750
BsiHKAI GWGCWC 2 cut(s) 1783, 2068
BsiHKCI CYCGRG 3 cut(s) 865, 1265, 1500
BsiSI CCGG 7 cut(s) 80, 101, 263, 683, 1501, 1639, 1755
BslFI GGGAC 5 cut(s) 62, 494, 572, 1487, 1664
BslI CCNNNNNNNGG 7 cut(s) 107, 239, 723, 1761, 1775, 1820, 2034
BsmAI GTCTC 2 cut(s) 263, 1041
BsmFI GGGAC 5 cut(s) 62, 494, 572, 1487, 1664
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 7 cut(s) 83, 104, 818, 1638, 1642, 1765, 1824
BsoBI CYCGRG 3 cut(s) 865, 1265, 1500
Bsp1286I GDGCHC 3 cut(s) 731, 1783, 2068
Bsp13I TCCGGA 1 cut(s) 682
Bsp1407I TGTACA 2 cut(s) 744, 2049
Bsp143I GATC 5 cut(s) 679, 1075, 1095, 1138, 2095
BspACI CCGC 4 cut(s) 477, 892, 1231, 1811
BspANI GGCC 7 cut(s) 83, 104, 818, 1638, 1642, 1765, 1824
BspCNI CTCAG 4 cut(s) 337, 343, 361, 990
BspDI ATCGAT 1 cut(s) 750
BspEI TCCGGA 1 cut(s) 682
BspFNI CGCG 2 cut(s) 894, 1233
BspHI TCATGA 1 cut(s) 1278
BspLI GGNNCC 6 cut(s) 130, 357, 482, 728, 1069, 2149
BspMAI CTGCAG 2 cut(s) 1426, 1519
BspPI GGATC 3 cut(s) 674, 1070, 1103
BspT107I GGYRCC 1 cut(s) 726
BsrDI GCAATG 1 cut(s) 1333
BsrFI RCCGGY 1 cut(s) 1638
BsrGI TGTACA 2 cut(s) 744, 2049
BsrI ACTGG 4 cut(s) 112, 404, 641, 1541
BssAI RCCGGY 1 cut(s) 1638
BssECI CCNNGG 4 cut(s) 169, 864, 1500, 1956
BssMI GATC 5 cut(s) 679, 1075, 1095, 1138, 2095
BssSI CACGAG 1 cut(s) 501
BssT1I CCWWGG 1 cut(s) 1956
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 3 cut(s) 820, 1041, 1563
Bst4CI ACNGT 6 cut(s) 94, 146, 448, 485, 1439, 2108
Bst6I CTCTTC 1 cut(s) 1346
BstAPI GCANNNNNTGC 1 cut(s) 1898
BstAUI TGTACA 2 cut(s) 744, 2049
BstBAI YACGTR 1 cut(s) 74
BstC8I GCNNGC 4 cut(s) 829, 951, 1636, 1640
BstDEI CTNAG 7 cut(s) 345, 351, 369, 832, 998, 1322, 1619
BstENI CCTNNNNNAGG 1 cut(s) 1818
BstF5I GGATG 9 cut(s) 231, 257, 418, 650, 664, 764, 1541, 1966, 2138
BstFNI CGCG 2 cut(s) 894, 1233
BstHHI GCGC 1 cut(s) 1810
BstKTI GATC 5 cut(s) 682, 1078, 1098, 1141, 2098
BstMAI GTCTC 2 cut(s) 263, 1041
BstMBI GATC 5 cut(s) 679, 1075, 1095, 1138, 2095
BstNI CCWGG 3 cut(s) 820, 1041, 1563
BstNSI RCATGY 3 cut(s) 632, 1418, 1480
BstPAI GACNNNNGTC 1 cut(s) 74
BstSCI CCNGG 6 cut(s) 78, 818, 1039, 1499, 1500, 1561
BstSFI CTRYAG 2 cut(s) 1422, 1515
BstSLI GKGCMC 1 cut(s) 731
BstUI CGCG 2 cut(s) 894, 1233
BstV1I GCAGC 7 cut(s) 39, 1305, 1370, 1408, 1501, 1529, 1578
BstV2I GAAGAC 3 cut(s) 154, 615, 1433
BstX2I RGATCY 1 cut(s) 1075
BstXI CCANNNNNNTGG 1 cut(s) 1528
BstYI RGATCY 1 cut(s) 1075
Bsu15I ATCGAT 1 cut(s) 750
BsuI GTATCC 2 cut(s) 179, 1963
BsuRI GGCC 7 cut(s) 83, 104, 818, 1638, 1642, 1765, 1824
BsuTUI ATCGAT 1 cut(s) 750
BtsCI GGATG 9 cut(s) 231, 257, 418, 650, 664, 764, 1541, 1966, 2138
BtsIMutI CAGTG 4 cut(s) 105, 444, 995, 1669
Cac8I GCNNGC 4 cut(s) 829, 951, 1636, 1640
CciI TCATGA 1 cut(s) 1278
CfoI GCGC 1 cut(s) 1810
Cfr10I RCCGGY 1 cut(s) 1638
Cfr13I GGNCC 2 cut(s) 49, 481
Cfr9I CCCGGG 1 cut(s) 1500
ClaI ATCGAT 1 cut(s) 750
Csp6I GTAC 8 cut(s) 591, 745, 1463, 1598, 1914, 2050, 2234, 2281
CviQI GTAC 8 cut(s) 591, 745, 1463, 1598, 1914, 2050, 2234, 2281
DdeI CTNAG 7 cut(s) 345, 351, 369, 832, 998, 1322, 1619
DpnI GATC 5 cut(s) 681, 1077, 1097, 1140, 2097
DpnII GATC 5 cut(s) 679, 1075, 1095, 1138, 2095
DrdI GACNNNNNNGTC 2 cut(s) 708, 1673
DseDI GACNNNNNNGTC 2 cut(s) 708, 1673
EaeI YGGCCR 5 cut(s) 81, 102, 816, 1636, 1763
Eam1104I CTCTTC 1 cut(s) 1346
EarI CTCTTC 1 cut(s) 1346
Eco130I CCWWGG 1 cut(s) 1956
Eco147I AGGCCT 1 cut(s) 1824
Eco47I GGWCC 2 cut(s) 49, 481
Eco57I CTGAAG 5 cut(s) 531, 950, 1011, 1016, 1715
Eco72I CACGTG 1 cut(s) 74
Eco88I CYCGRG 3 cut(s) 865, 1265, 1500
EcoNI CCTNNNNNAGG 1 cut(s) 1818
EcoRI GAATTC 1 cut(s) 526
EcoRII CCWGG 3 cut(s) 818, 1039, 1561
EcoT14I CCWWGG 1 cut(s) 1956
EcoT22I ATGCAT 3 cut(s) 664, 946, 1903
ErhI CCWWGG 1 cut(s) 1956
FaqI GGGAC 5 cut(s) 62, 494, 572, 1487, 1664
FauI CCCGC 1 cut(s) 470
FauNDI CATATG 1 cut(s) 1061
FblI GTMKAC 2 cut(s) 633, 2208
Fnu4HI GCNGC 8 cut(s) 28, 1294, 1359, 1422, 1515, 1518, 1567, 1811
FokI GGATG 9 cut(s) 238, 264, 425, 657, 671, 771, 1548, 1973, 2145
FseI GGCCGGCC 1 cut(s) 1642
Fsp4HI GCNGC 8 cut(s) 28, 1294, 1359, 1422, 1515, 1518, 1567, 1811
FspBI CTAG 6 cut(s) 1073, 1391, 1626, 1647, 1967, 2078
GlaI GCGC 1 cut(s) 1809
GluI GCNGC 8 cut(s) 28, 1294, 1359, 1422, 1515, 1518, 1567, 1811
HaeIII GGCC 7 cut(s) 83, 104, 818, 1638, 1642, 1765, 1824
HapII CCGG 7 cut(s) 80, 101, 263, 683, 1501, 1639, 1755
HhaI GCGC 1 cut(s) 1810
Hin6I GCGC 1 cut(s) 1808
HinP1I GCGC 1 cut(s) 1808
HindIII AAGCTT 2 cut(s) 829, 1592
HpaII CCGG 7 cut(s) 80, 101, 263, 683, 1501, 1639, 1755
HphI GGTGA 4 cut(s) 82, 122, 1975, 2021
Hpy166II GTNNAC 3 cut(s) 634, 2185, 2209
Hpy8I GTNNAC 3 cut(s) 634, 2185, 2209
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 8 cut(s) 74, 680, 713, 749, 1122, 1174, 2027, 2110
HpyCH4III ACNGT 6 cut(s) 94, 146, 448, 485, 1439, 2108
HpyCH4IV ACGT 4 cut(s) 73, 302, 675, 1056
HpyF3I CTNAG 7 cut(s) 345, 351, 369, 832, 998, 1322, 1619
HpySE526I ACGT 4 cut(s) 73, 302, 675, 1056
HspAI GCGC 1 cut(s) 1808
Kpn2I TCCGGA 1 cut(s) 682
KroI GCCGGC 1 cut(s) 1638
KroNI GCCGGC 1 cut(s) 1640
Kzo9I GATC 5 cut(s) 679, 1075, 1095, 1138, 2095
LmnI GCTCC 5 cut(s) 128, 355, 416, 2063, 2147
Lsp1109I GCAGC 7 cut(s) 39, 1305, 1370, 1408, 1501, 1529, 1578
LweI GCATC 2 cut(s) 649, 2123
MaeI CTAG 6 cut(s) 1073, 1391, 1626, 1647, 1967, 2078
MaeII ACGT 4 cut(s) 73, 302, 675, 1056
MaeIII GTNAC 8 cut(s) 53, 94, 505, 1043, 1052, 1553, 1664, 2009
MalI GATC 5 cut(s) 681, 1077, 1097, 1140, 2097
MboI GATC 5 cut(s) 679, 1075, 1095, 1138, 2095
MboII GAAGA 8 cut(s) 154, 620, 628, 848, 1363, 1433, 1708, 1868
MfeI CAATTG 2 cut(s) 1741, 1770
MflI RGATCY 1 cut(s) 1075
MhlI GDGCHC 3 cut(s) 731, 1783, 2068
MlsI TGGCCA 2 cut(s) 818, 1765
MluCI AATT 7 cut(s) 526, 774, 1573, 1741, 1770, 2021, 2053
MluNI TGGCCA 2 cut(s) 818, 1765
MlyI GAGTC 7 cut(s) 265, 356, 554, 696, 1236, 1327, 2133
MmeI TCCRAC 2 cut(s) 1007, 1856
Mox20I TGGCCA 2 cut(s) 818, 1765
Mph1103I ATGCAT 3 cut(s) 664, 946, 1903
MroI TCCGGA 1 cut(s) 682
MroNI GCCGGC 1 cut(s) 1638
MscI TGGCCA 2 cut(s) 818, 1765
MseI TTAA 5 cut(s) 450, 567, 1434, 1845, 2238
Msp20I TGGCCA 2 cut(s) 818, 1765
MspI CCGG 7 cut(s) 80, 101, 263, 683, 1501, 1639, 1755
MspR9I CCNGG 6 cut(s) 80, 820, 1041, 1501, 1502, 1563
MunI CAATTG 2 cut(s) 1741, 1770
Mva1269I GAATGC 1 cut(s) 419
MvaI CCWGG 3 cut(s) 820, 1041, 1563
MvnI CGCG 2 cut(s) 894, 1233
NaeI GCCGGC 1 cut(s) 1640
NciI CCSGG 3 cut(s) 80, 1501, 1502
NdeI CATATG 1 cut(s) 1061
NdeII GATC 5 cut(s) 679, 1075, 1095, 1138, 2095
NgoMIV GCCGGC 1 cut(s) 1638
NlaIV GGNNCC 6 cut(s) 130, 357, 482, 728, 1069, 2149
NmuCI GTSAC 5 cut(s) 53, 94, 1553, 1664, 2009
NsiI ATGCAT 3 cut(s) 664, 946, 1903
NspI RCATGY 3 cut(s) 632, 1418, 1480
PaeR7I CTCGAG 1 cut(s) 1265
PagI TCATGA 1 cut(s) 1278
PceI AGGCCT 1 cut(s) 1824
PciI ACATGT 3 cut(s) 628, 1414, 1476
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PctI GAATGC 1 cut(s) 419
PdiI GCCGGC 1 cut(s) 1640
PkrI GCNGC 8 cut(s) 29, 1295, 1360, 1423, 1516, 1519, 1568, 1812
PleI GAGTC 7 cut(s) 265, 355, 554, 696, 1235, 1326, 2132
PmaCI CACGTG 1 cut(s) 74
PmlI CACGTG 1 cut(s) 74
PpsI GAGTC 7 cut(s) 265, 355, 554, 696, 1235, 1326, 2132
Ppu21I YACGTR 1 cut(s) 74
PscI ACATGT 3 cut(s) 628, 1414, 1476
PshAI GACNNNNGTC 1 cut(s) 74
PshBI ATTAAT 1 cut(s) 1845
PsiI TTATAA 1 cut(s) 669
Psp6I CCWGG 3 cut(s) 818, 1039, 1561
PspCI CACGTG 1 cut(s) 74
PspGI CCWGG 3 cut(s) 818, 1039, 1561
PspN4I GGNNCC 6 cut(s) 130, 357, 482, 728, 1069, 2149
PspPI GGNCC 2 cut(s) 49, 481
PspXI VCTCGAGB 1 cut(s) 1265
PstI CTGCAG 2 cut(s) 1426, 1519
PsuI RGATCY 1 cut(s) 1075
RigI GGCCGGCC 1 cut(s) 1642
RsaI GTAC 8 cut(s) 592, 746, 1464, 1599, 1915, 2051, 2235, 2282
RsaNI GTAC 8 cut(s) 591, 745, 1463, 1598, 1914, 2050, 2234, 2281
SaqAI TTAA 5 cut(s) 450, 567, 1434, 1845, 2238
SatI GCNGC 8 cut(s) 28, 1294, 1359, 1422, 1515, 1518, 1567, 1811
Sau3AI GATC 5 cut(s) 679, 1075, 1095, 1138, 2095
Sau96I GGNCC 2 cut(s) 49, 481
SchI GAGTC 7 cut(s) 265, 356, 554, 696, 1236, 1327, 2133
ScrFI CCNGG 6 cut(s) 80, 820, 1041, 1501, 1502, 1563
SduI GDGCHC 3 cut(s) 731, 1783, 2068
SfaNI GCATC 2 cut(s) 649, 2123
SfcI CTRYAG 2 cut(s) 1422, 1515
Sfr274I CTCGAG 1 cut(s) 1265
SinI GGWCC 2 cut(s) 49, 481
SlaI CTCGAG 1 cut(s) 1265
SmaI CCCGGG 1 cut(s) 1502
SmlI CTYRAG 3 cut(s) 179, 458, 1265
SmoI CTYRAG 3 cut(s) 179, 458, 1265
Sse9I AATT 7 cut(s) 526, 774, 1573, 1741, 1770, 2021, 2053
SseBI AGGCCT 1 cut(s) 1824
SsiI CCGC 4 cut(s) 477, 892, 1231, 1811
SspMI CTAG 6 cut(s) 1073, 1391, 1626, 1647, 1967, 2078
StuI AGGCCT 1 cut(s) 1824
StyD4I CCNGG 6 cut(s) 78, 818, 1039, 1499, 1500, 1561
StyI CCWWGG 1 cut(s) 1956
TaaI ACNGT 6 cut(s) 94, 146, 448, 485, 1439, 2108
TaiI ACGT 4 cut(s) 76, 305, 678, 1059
TaqI TCGA 8 cut(s) 305, 750, 1115, 1266, 1677, 2103, 2122, 2140
TasI AATT 7 cut(s) 526, 774, 1573, 1741, 1770, 2021, 2053
TatI WGTACW 4 cut(s) 744, 1597, 2049, 2280
TauI GCSGC 1 cut(s) 1813
Tru1I TTAA 5 cut(s) 450, 567, 1434, 1845, 2238
Tru9I TTAA 5 cut(s) 450, 567, 1434, 1845, 2238
TscAI CASTG 4 cut(s) 112, 451, 1002, 1669
TseFI GTSAC 5 cut(s) 53, 94, 1553, 1664, 2009
TseI GCWGC 7 cut(s) 27, 1293, 1358, 1421, 1514, 1517, 1566
Tsp45I GTSAC 5 cut(s) 53, 94, 1553, 1664, 2009
TspDTI ATGAA 9 cut(s) 34, 112, 768, 798, 954, 1212, 1372, 1884, 2266
TspGWI ACGGA 2 cut(s) 41, 1188
TspMI CCCGGG 1 cut(s) 1500
TspRI CASTG 4 cut(s) 112, 451, 1002, 1669
VpaK11BI GGWCC 2 cut(s) 49, 481
VspI ATTAAT 1 cut(s) 1845
XagI CCTNNNNNAGG 1 cut(s) 1818
XapI RAATTY 1 cut(s) 526
XbaI TCTAGA 1 cut(s) 2077
XceI RCATGY 3 cut(s) 632, 1418, 1480
XhoI CTCGAG 1 cut(s) 1265
XmaI CCCGGG 1 cut(s) 1500
XmiI GTMKAC 2 cut(s) 633, 2208
XspI CTAG 6 cut(s) 1073, 1391, 1626, 1647, 1967, 2078
Zsp2I ATGCAT 3 cut(s) 664, 946, 1903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.