Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0000881

Physical Location & Seq

Genomic Coordinates Forward (+)

27390038 .. 27409402

Gene Structure 19365 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG279100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2466 bp

ATGGGCTCCGATGACCTCTCCACCGTGTCCGCCGCCACTGACGATGAGAAGTACGGGTTCAAGAGGCCGGAGACCACGCCGTCCATACATATCTATCGTTGTATTCCCAAAGGGTTAAGAAAGCTCTGCCTTCTGTACTTGGGTGCTCTCTCAAAGTCTCAATCAACCATGACTGTCACTCCAAACATTTCCATCAACGATGGAAACCTGGTGGTTCACGGAAAGACCATATTGACCGGAGTGCCGGAAAACATTGTGCTCACTCCAGGATCCGGTGTCGGCCTCGTTGCTGGTGCTTTCATTGGCGCCACCGCCTCTCACAGCAAAAGCCTCCATGTCTTCCCTGTCGGTGTTTTAGAGGGTCAGCGGTTCATGTGCCTTTTCCGGTTTAAGCTATGGTGGATGACGCAGAGGATGGGGTCGTGTGGGAAAGAAGTTCCACTGGAGACCCAATTCATGCTTGTGGAGACCAAGGATGATGGTGAAGGAAGTGATGAATCCTCCACCATTTACACCGTCTTCCTTCCTCTCCTGGAGGGCCAGTTCCGTTCGGTTCTCCAAGGAAACGAGCGGAATGAAGTGGAGATATGCCTCGAGAGTGGAGATTCTGATGTTCAAACCAATCAAGGTCTTTGCCTTGTTTACGTGCATGCTGGAACTAATCCATTTGAAGTTATCAACCAGGCTGTAAAGGCTGTAGAAAAACACATGCAAACCTTTGTCCATCGTGAGAAGAAAAAGTTGCCTTCTTTCCTCGACTGGTTTGGCTGGTGCACATGGGATGCTTTCTATACCGATGTCACAGCTGAGGGTGTCGTGGATGGTCTTAAAAGCTTGAATGAGGGAGGGACGCCTACGCGATTTCTAATCGTAGATGATGGTTGGCAACAAATTGAAAGTAAACCCAAGGATAGTAATGTTGTAGTACAAGAAGGAGCACAGTTTGCAAGTATGCTTACAGGAATAAAAGAGAATGAAAAATTCCAAAAGAATGGACAGAGTGAGCAAGTCTCAGGCCTGAAACATGTTGTTGATGAAGCGAAGCAGCATCACAATGTGAAGTTTGTGTATGTATGGCATGCTTTAGCTGGGTACTGGGGTGGAGTGAAACCAGCTGCTTCTGGCATGGAGCATTATGACACTGCTTTGGCATATCCAGTCTCATCTCCTGGTGTGATGGGAAACCAACCTGACATAGTTTTTGACAGCTTATCTGTTCACGGTCTTGGTTTGGTGCATCCAAAGAAAGTTTTCAATTTCTACAATGAGCTTCATGCGTATTTGGCTTCCTGTGGAGTGGATGGAGTTAAGGTTGATGTACAGAACATCATTGAGACTCTTGGGTCTGGTCATGGTGGCAGAGTTTCTCTGACCCGAAGCTATCACCAGGCACTTGAGGCTTCGGTTGCTCGGAACTTTCCTGACAACGGATGCATTTCTTGCATGTGTCATAACACTGATGGGCTATATAGTTCCAAGCAGACTGCTGTTGTGAGAGCTTCTGATGACTTCTACCCACGGGATCCTGCTTCTCACACGATTCATATATCTTCTGTGGCTTATAACACCCTTTTCCTTGGCGAGTTTATGCAACCAGACTGGGATATGTTTCATAGTTTACATCCAGCAGCGGATTATCATGGTGCAGCTCGTTCTATTGGTGGATGTGCAATCTATGTCAGTGATAAACCTGGAAACCACAACTTCGATCTTTTGAGGAAGCTGGTCCTGCCTGATGGATCTGTCCTCCGTGCTCAATTACCTGGTAGGCCAACACGTGATTGTCTCTTTGCTGACCCAGCAAGAGATGGAACCAGCTTGCTCAAGATATGGAATGTGAACAAGTGCTCTGGTGTTGTTGGTGTATTCAACTGTCAAGGGGCTGGTTGGTGCAAGGTTGAAAAGAAGATCCGCATCCATGATGCATCTCCTGGTACCCTCACTGGTTCTGTTCGTGCTATTGATGTTGATAGCCTTGCCCAAATTGCTGGTGATGATTGGAATGGAGAAACAGTAGTTTATGCCCATAAGTCAGGAGAGGTGTTTCGTTTGCCAAAAGATGTTTCAGTGCCAGTGACACTTAAAGTCCTGGAGTATGAGCTCTTCCATTTCTGTCCTCTCAAGGAGGTTACACCCAACATCTCATTTGCTCCAATAGGCCTGCTTGACATGTTCAACTCCAGTGGTGCTGTGGAGCATGTTGTAATCCATCTGGCTTCTGACGGCGAAGTTTCTTCGGAGCTTACCACTTCTCTCAGTGAGAATAGATCTTCAACTGCCACAATCACCATAAAAACCAGAGGATGTGGAAGGTTTGGAGCTTACTCTTCTCAGCGTCCCCTGAAGTGTACCGTTGATAGAGTTGAGACGGACTTTGAATATGACCCTCCAACTGGATTACTAACCTTTACCATCCCTATCCCAAAAGAGGATATGTATAAATGGCTAGTAGAAATCCAAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005911 GO:0005975 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009506 GO:0009628 GO:0015925 GO:0016052 GO:0016787 GO:0016798 GO:0030054 GO:0033530 GO:0034484 GO:0044238 GO:0050896 GO:0052692 GO:0055044 GO:0071704 GO:0080167 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00052 map00052

Pfam Domains

Protein Families

Raffinose_syn

Protein Analysis

821

Amino Acids

90.01

Weight (kDa)

5.81

Isoelectric Point (pI)

31.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Raffinose_syn	PF05691	63 - 804	0.0	Raffinose synthase or seed imbibition protein Sip1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57520 AT3G57520 AT3G57520
fragaria_vesca	FvH4_3g00160 FvH4_6g23550 FvH4_6g23550 FvH4_6g23550 FvH4_6g23550
malus_domestica	MD09G1161700.v1.1 MD09G1162100.v1.1 MD10G1341400.v1.1 MD10G1341500.v1.1 MD10G1341600.v1.1 MD12G1077800.v1.1 MD14G1070300.v1.1
prunus_persica	Prupe.4G001700_v2.0.a1 Prupe.5G162400_v2.0.a1 Prupe.5G162400_v2.0.a1 Prupe.7G064000_v2.0.a1 Prupe.7G064000_v2.0.a1
pyrus_communis	pycom10g29090 pycom12g06210 pycom14g05800 pycom14g05880 pycom14g05890 pycom14g05910 pycom14g05930
rosa_chinensis	RchiOBHm_Chr3g0481621 RchiOBHm_Chr5g0000211 RchiOBHm_Chr7g0192011 RchiOBHm_Chr7g0192021
rosa_laevigata	RLG00000004409 RLG00000023385 RLG00000030850
rosa_multiflora	Rmu_co8093432.1_g000001 Rmu_co8314355.1_g000001 Rmu_sc0010409.1_g000002 Rmu_sc0021936.1_g000002 Rmu_ssc0000243.1_g000010 Rmu_ssc0000421.1_g000033
rosa_roxburghii	Rroxscaffold_1G00075890 Rroxscaffold_3G00263360 Rroxscaffold_6G00400310
rosa_rugosa	Rorug03G0192700 Rorug03G0192800 Rorug03G0192900 Rorug04G0382500 Rorug06G0512300 Rorug06G0512300
rosa_samantha	Rh3AG244100 Rh3BG279100 Rh3BG279300 Rh3CG274100 Rh3DG270900 Rh5AG001400 Rh5BG002800 Rh5CG001700 Rh5DG001600 Rh7AG122700 Rh7AG122800 Rh7BG124300 Rh7CG127000 Rh7DG125800
rosa_wichuraiana	Rw3G021800 Rw5G000190 Rw7G010450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1563
AasI	GACNNNNNNGTC	2 cut(s)	79, 1342
Acc65I	GGTACC	1 cut(s)	1934
AccB1I	GGYRCC	2 cut(s)	305, 1934
AccBSI	CCGCTC	1 cut(s)	571
AccII	CGCG	1 cut(s)	859
AciI	CCGC	7 cut(s)	30, 33, 312, 367, 571, 1631, 1912
AclWI	GGATC	6 cut(s)	264, 277, 1517, 1530, 1747, 1903
AcsI	RAATTY	1 cut(s)	980
AcuI	CTGAAG	1 cut(s)	2363
AcvI	CACGTG	1 cut(s)	1778
AcyI	GRCGYC	2 cut(s)	306, 851
AdeI	CACNNNGTG	1 cut(s)	1057
AfaI	GTAC	7 cut(s)	53, 137, 927, 1094, 1320, 1936, 2350
AfiI	CCNNNNNNNGG	4 cut(s)	272, 1427, 2393, 2428
AflIII	ACRYGT	3 cut(s)	1024, 1775, 2169
AjuI	GAANNNNNNNTTGG	2 cut(s)	2047, 2079
AloI	GAACNNNNNNTCC	2 cut(s)	527, 559
Alw21I	GWGCWC	7 cut(s)	148, 261, 776, 940, 1756, 1850, 2103
Alw26I	GTCTC	9 cut(s)	65, 162, 440, 461, 1015, 1165, 1328, 1790, 2360
Alw44I	GTGCAC	1 cut(s)	772
AlwI	GGATC	6 cut(s)	264, 277, 1517, 1530, 1747, 1903
Ama87I	CYCGRG	1 cut(s)	593
AoxI	GGCC	6 cut(s)	65, 280, 538, 1015, 1769, 2158
ApaLI	GTGCAC	1 cut(s)	772
ApeKI	GCWGC	4 cut(s)	1045, 1115, 1628, 1646
ApoI	RAATTY	1 cut(s)	980
ArsI	GACNNNNNNTTYG	2 cut(s)	1184, 1216
Asp700I	GAANNNNTTC	1 cut(s)	1250
Asp718I	GGTACC	1 cut(s)	1934
AspLEI	GCGC	1 cut(s)	308
AspS9I	GGNCC	2 cut(s)	538, 1726
AsuHPI	GGTGA	4 cut(s)	494, 1376, 2003, 2278
AvaI	CYCGRG	1 cut(s)	593
AvaII	GGWCC	1 cut(s)	1726
BaeGI	GKGCMC	1 cut(s)	776
BaeI	ACNNNNGTAYC	2 cut(s)	1084, 1117
BamHI	GGATCC	2 cut(s)	269, 1522
BanI	GGYRCC	2 cut(s)	305, 1934
BanII	GRGCYC	2 cut(s)	8, 2103
BarI	GAAGNNNNNNTAC	2 cut(s)	2087, 2119
BbrPI	CACGTG	1 cut(s)	1778
BbsI	GAAGAC	2 cut(s)	331, 511
Bbv12I	GWGCWC	7 cut(s)	148, 261, 776, 940, 1756, 1850, 2103
BbvCI	CCTCAGC	1 cut(s)	807
BbvI	GCAGC	4 cut(s)	1057, 1102, 1640, 1658
BceAI	ACGGC	2 cut(s)	64, 2239
BcoDI	GTCTC	9 cut(s)	65, 162, 440, 461, 1015, 1165, 1328, 1790, 2360
BfaI	CTAG	1 cut(s)	2447
BfmI	CTRYAG	1 cut(s)	696
BfoI	RGCGCY	1 cut(s)	309
BglII	AGATCT	1 cut(s)	2267
BisI	GCNGC	5 cut(s)	33, 1046, 1116, 1629, 1647
BlsI	GCNGC	5 cut(s)	34, 1047, 1117, 1630, 1648
Bme18I	GGWCC	1 cut(s)	1726
BmeT110I	CYCGRG	1 cut(s)	593
BmgT120I	GGNCC	2 cut(s)	538, 1726
BmiI	GGNNCC	6 cut(s)	7, 271, 307, 1524, 1813, 1936
BmrI	ACTGGG	2 cut(s)	1105, 1609
BmsI	GCATC	7 cut(s)	772, 1057, 1246, 1421, 1912, 1923, 1934
BmuI	ACTGGG	2 cut(s)	1105, 1609
BpiI	GAAGAC	2 cut(s)	331, 511
BplI	GAGNNNNNCTC	2 cut(s)	995, 1027
BpmI	CTGGAG	5 cut(s)	249, 464, 554, 2111, 2164
Bpu10I	CCTNAGC	1 cut(s)	807
BpuEI	CTTGAG	3 cut(s)	1415, 1808, 2105
BsaAI	YACGTR	2 cut(s)	646, 1778
BsaBI	GATNNNNATC	1 cut(s)	1913
BsaHI	GRCGYC	2 cut(s)	306, 851
BsaI	GGTCTC	3 cut(s)	65, 440, 461
BsaJI	CCNNGG	5 cut(s)	471, 559, 906, 1517, 1576
BsaWI	WCCGGW	3 cut(s)	236, 272, 384
BsaXI	ACNNNNNCTCC	8 cut(s)	163, 193, 527, 557, 1296, 1326, 1998, 2028
Bsc4I	CCNNNNNNNGG	4 cut(s)	272, 1427, 2393, 2428
Bse8I	GATNNNNATC	1 cut(s)	1913
BseDI	CCNNGG	5 cut(s)	471, 559, 906, 1517, 1576
BseJI	GATNNNNATC	1 cut(s)	1913
BseLI	CCNNNNNNNGG	4 cut(s)	272, 1427, 2393, 2428
BseMII	CTCAG	4 cut(s)	798, 1026, 2269, 2345
BseSI	GKGCMC	1 cut(s)	776
BseXI	GCAGC	4 cut(s)	1057, 1102, 1640, 1658
BseYI	CCCAGC	2 cut(s)	1088, 1798
BsgI	GTGCAG	1 cut(s)	1665
Bsh1236I	CGCG	1 cut(s)	859
BshFI	GGCC	6 cut(s)	67, 282, 540, 1017, 1771, 2160
BshNI	GGYRCC	2 cut(s)	305, 1934
BsiHKAI	GWGCWC	7 cut(s)	148, 261, 776, 940, 1756, 1850, 2103
BsiHKCI	CYCGRG	1 cut(s)	593
BsiSI	CCGG	5 cut(s)	68, 237, 245, 273, 385
BslFI	GGGAC	2 cut(s)	862, 2322
BslI	CCNNNNNNNGG	4 cut(s)	272, 1427, 2393, 2428
BsmAI	GTCTC	9 cut(s)	65, 162, 440, 461, 1015, 1165, 1328, 1790, 2360
BsmBI	CGTCTC	1 cut(s)	2360
BsmFI	GGGAC	2 cut(s)	862, 2322
BsnI	GGCC	6 cut(s)	67, 282, 540, 1017, 1771, 2160
Bso31I	GGTCTC	3 cut(s)	65, 440, 461
BsoBI	CYCGRG	1 cut(s)	593
Bsp1286I	GDGCHC	8 cut(s)	8, 148, 261, 776, 940, 1756, 1850, 2103
Bsp1407I	TGTACA	1 cut(s)	1318
Bsp143I	GATC	6 cut(s)	269, 1522, 1708, 1739, 1908, 2267
BspACI	CCGC	7 cut(s)	30, 33, 312, 367, 571, 1631, 1912
BspANI	GGCC	6 cut(s)	67, 282, 540, 1017, 1771, 2160
BspCNI	CTCAG	4 cut(s)	799, 1025, 2268, 2344
BspFNI	CGCG	1 cut(s)	859
BspLI	GGNNCC	6 cut(s)	7, 271, 307, 1524, 1813, 1936
BspPI	GGATC	6 cut(s)	264, 277, 1517, 1530, 1747, 1903
BspQI	GCTCTTC	1 cut(s)	2108
BspT107I	GGYRCC	2 cut(s)	305, 1934
BspTNI	GGTCTC	3 cut(s)	65, 440, 461
BsrBI	CCGCTC	1 cut(s)	571
BsrGI	TGTACA	1 cut(s)	1318
BssECI	CCNNGG	5 cut(s)	471, 559, 906, 1517, 1576
BssMI	GATC	6 cut(s)	269, 1522, 1708, 1739, 1908, 2267
BssNI	GRCGYC	2 cut(s)	306, 851
BssT1I	CCWWGG	4 cut(s)	471, 559, 906, 1576
Bst4CI	ACNGT	8 cut(s)	25, 175, 517, 942, 1223, 1874, 2014, 2353
Bst6I	CTCTTC	2 cut(s)	2108, 2332
BstACI	GRCGYC	2 cut(s)	306, 851
BstAPI	GCANNNNNTGC	2 cut(s)	944, 1440
BstAUI	TGTACA	1 cut(s)	1318
BstBAI	YACGTR	2 cut(s)	646, 1778
BstC8I	GCNNGC	4 cut(s)	651, 1080, 1820, 2162
BstDEI	CTNAG	4 cut(s)	807, 1012, 2255, 2331
BstDSI	CCRYGG	1 cut(s)	1517
BstFNI	CGCG	1 cut(s)	859
BstH2I	RGCGCY	1 cut(s)	309
BstHHI	GCGC	1 cut(s)	308
BstKTI	GATC	6 cut(s)	272, 1525, 1711, 1742, 1911, 2270
BstMAI	GTCTC	9 cut(s)	65, 162, 440, 461, 1015, 1165, 1328, 1790, 2360
BstMBI	GATC	6 cut(s)	269, 1522, 1708, 1739, 1908, 2267
BstMWI	GCNNNNNNNGC	9 cut(s)	692, 944, 1283, 1397, 1406, 1440, 1729, 1799, 1985
BstNSI	RCATGY	7 cut(s)	653, 712, 1028, 1082, 1447, 2173, 2201
BstSFI	CTRYAG	1 cut(s)	696
BstSLI	GKGCMC	1 cut(s)	776
BstUI	CGCG	1 cut(s)	859
BstV1I	GCAGC	4 cut(s)	1057, 1102, 1640, 1658
BstV2I	GAAGAC	2 cut(s)	331, 511
BstX2I	RGATCY	5 cut(s)	269, 1522, 1739, 1908, 2267
BstXI	CCANNNNNNTGG	2 cut(s)	992, 1988
BstYI	RGATCY	5 cut(s)	269, 1522, 1739, 1908, 2267
BsuRI	GGCC	6 cut(s)	67, 282, 540, 1017, 1771, 2160
BtgI	CCRYGG	1 cut(s)	1517
BtsI	GCAGTG	1 cut(s)	1140
Cac8I	GCNNGC	4 cut(s)	651, 1080, 1820, 2162
CfoI	GCGC	1 cut(s)	308
Cfr13I	GGNCC	2 cut(s)	538, 1726
CseI	GACGC	3 cut(s)	415, 859, 2324
CsiI	ACCWGGT	2 cut(s)	207, 1762
Csp6I	GTAC	7 cut(s)	52, 136, 926, 1093, 1319, 1935, 2349
CspCI	CAANNNNNGTGG	2 cut(s)	2164, 2199
CviQI	GTAC	7 cut(s)	52, 136, 926, 1093, 1319, 1935, 2349
DdeI	CTNAG	4 cut(s)	807, 1012, 2255, 2331
DinI	GGCGCC	1 cut(s)	307
DpnI	GATC	6 cut(s)	271, 1524, 1710, 1741, 1910, 2269
DpnII	GATC	6 cut(s)	269, 1522, 1708, 1739, 1908, 2267
DraIII	CACNNNGTG	1 cut(s)	1057
DrdI	GACNNNNNNGTC	2 cut(s)	79, 1342
DseDI	GACNNNNNNGTC	2 cut(s)	79, 1342
Eam1104I	CTCTTC	2 cut(s)	2108, 2332
EarI	CTCTTC	2 cut(s)	2108, 2332
EciI	GGCGGA	1 cut(s)	19
Ecl136II	GAGCTC	1 cut(s)	2101
Eco130I	CCWWGG	4 cut(s)	471, 559, 906, 1576
Eco147I	AGGCCT	2 cut(s)	1017, 2160
Eco24I	GRGCYC	2 cut(s)	8, 2103
Eco31I	GGTCTC	3 cut(s)	65, 440, 461
Eco47I	GGWCC	1 cut(s)	1726
Eco53kI	GAGCTC	1 cut(s)	2101
Eco57I	CTGAAG	1 cut(s)	2363
Eco72I	CACGTG	1 cut(s)	1778
Eco88I	CYCGRG	1 cut(s)	593
EcoICRI	GAGCTC	1 cut(s)	2101
EcoT14I	CCWWGG	4 cut(s)	471, 559, 906, 1576
EcoT22I	ATGCAT	2 cut(s)	1436, 1927
EcoT38I	GRGCYC	2 cut(s)	8, 2103
EgeI	GGCGCC	1 cut(s)	307
EheI	GGCGCC	1 cut(s)	307
ErhI	CCWWGG	4 cut(s)	471, 559, 906, 1576
Esp3I	CGTCTC	1 cut(s)	2360
FaqI	GGGAC	2 cut(s)	862, 2322
Fnu4HI	GCNGC	5 cut(s)	33, 1046, 1116, 1629, 1647
FriOI	GRGCYC	2 cut(s)	8, 2103
Fsp4HI	GCNGC	5 cut(s)	33, 1046, 1116, 1629, 1647
FspBI	CTAG	1 cut(s)	2447
GlaI	GCGC	1 cut(s)	307
GluI	GCNGC	5 cut(s)	33, 1046, 1116, 1629, 1647
GsaI	CCCAGC	2 cut(s)	1092, 1802
GsuI	CTGGAG	5 cut(s)	249, 464, 554, 2111, 2164
HaeII	RGCGCY	1 cut(s)	309
HaeIII	GGCC	6 cut(s)	67, 282, 540, 1017, 1771, 2160
HapII	CCGG	5 cut(s)	68, 237, 245, 273, 385
HgaI	GACGC	3 cut(s)	415, 859, 2324
HhaI	GCGC	1 cut(s)	308
Hin1I	GRCGYC	2 cut(s)	306, 851
Hin6I	GCGC	1 cut(s)	306
HinP1I	GCGC	1 cut(s)	306
HindIII	AAGCTT	1 cut(s)	832
HinfI	GANTC	4 cut(s)	497, 605, 1336, 1540
HpaII	CCGG	5 cut(s)	68, 237, 245, 273, 385
HphI	GGTGA	4 cut(s)	494, 1376, 2003, 2278
Hpy166II	GTNNAC	8 cut(s)	217, 643, 774, 902, 1219, 1619, 1840, 2349
Hpy188I	TCNGA	7 cut(s)	10, 610, 1371, 1413, 1504, 2221, 2239
Hpy188III	TCNNGA	6 cut(s)	61, 595, 728, 1421, 1825, 2034
Hpy8I	GTNNAC	8 cut(s)	217, 643, 774, 902, 1219, 1619, 1840, 2349
HpyAV	CCTTC	6 cut(s)	140, 479, 533, 756, 926, 2304
HpyCH4III	ACNGT	8 cut(s)	25, 175, 517, 942, 1223, 1874, 2014, 2353
HpyCH4IV	ACGT	2 cut(s)	645, 1777
HpyF10VI	GCNNNNNNNGC	9 cut(s)	692, 944, 1283, 1397, 1406, 1440, 1729, 1799, 1985
HpyF3I	CTNAG	4 cut(s)	807, 1012, 2255, 2331
HpySE526I	ACGT	2 cut(s)	645, 1777
Hsp92I	GRCGYC	2 cut(s)	306, 851
HspAI	GCGC	1 cut(s)	306
KasI	GGCGCC	1 cut(s)	305
KpnI	GGTACC	1 cut(s)	1938
Kzo9I	GATC	6 cut(s)	269, 1522, 1708, 1739, 1908, 2267
LguI	GCTCTTC	1 cut(s)	2108
LmnI	GCTCC	7 cut(s)	11, 935, 1129, 2155, 2194, 2239, 2318
Lsp1109I	GCAGC	4 cut(s)	1057, 1102, 1640, 1658
LweI	GCATC	7 cut(s)	772, 1057, 1246, 1421, 1912, 1923, 1934
MabI	ACCWGGT	2 cut(s)	207, 1762
MaeI	CTAG	1 cut(s)	2447
MaeII	ACGT	2 cut(s)	645, 1777
MaeIII	GTNAC	4 cut(s)	175, 799, 2074, 2128
MalI	GATC	6 cut(s)	271, 1524, 1710, 1741, 1910, 2269
MbiI	CCGCTC	1 cut(s)	571
MboI	GATC	6 cut(s)	269, 1522, 1708, 1739, 1908, 2267
MboII	GAAGA	9 cut(s)	331, 511, 745, 1542, 1918, 2095, 2226, 2262, 2319
MflI	RGATCY	5 cut(s)	269, 1522, 1739, 1908, 2267
MhlI	GDGCHC	8 cut(s)	8, 148, 261, 776, 940, 1756, 1850, 2103
MluCI	AATT	6 cut(s)	452, 891, 980, 1255, 1757, 1983
Mly113I	GGCGCC	1 cut(s)	306
MlyI	GAGTC	1 cut(s)	1330
MmeI	TCCRAC	1 cut(s)	2414
Mph1103I	ATGCAT	2 cut(s)	1436, 1927
MroXI	GAANNNNTTC	1 cut(s)	1250
MseI	TTAA	6 cut(s)	116, 390, 828, 1308, 2082, 2464
MslI	CAYNNNNRTG	2 cut(s)	461, 1053
MspA1I	CMGCKG	4 cut(s)	367, 806, 1115, 1631
MspI	CCGG	5 cut(s)	68, 237, 245, 273, 385
MvnI	CGCG	1 cut(s)	859
MwoI	GCNNNNNNNGC	9 cut(s)	692, 944, 1283, 1397, 1406, 1440, 1729, 1799, 1985
NarI	GGCGCC	1 cut(s)	306
NdeII	GATC	6 cut(s)	269, 1522, 1708, 1739, 1908, 2267
NlaIV	GGNNCC	6 cut(s)	7, 271, 307, 1524, 1813, 1936
NmuCI	GTSAC	3 cut(s)	175, 799, 2074
NsiI	ATGCAT	2 cut(s)	1436, 1927
NspI	RCATGY	7 cut(s)	653, 712, 1028, 1082, 1447, 2173, 2201
PaeI	GCATGC	2 cut(s)	653, 1082
PaeR7I	CTCGAG	1 cut(s)	593
PceI	AGGCCT	2 cut(s)	1017, 2160
PciI	ACATGT	2 cut(s)	1024, 2169
PciSI	GCTCTTC	1 cut(s)	2108
PdmI	GAANNNNTTC	1 cut(s)	1250
PfeI	GAWTC	3 cut(s)	497, 605, 1540
PfoI	TCCNGGA	3 cut(s)	265, 531, 2088
PkrI	GCNGC	5 cut(s)	34, 1047, 1117, 1630, 1648
PleI	GAGTC	1 cut(s)	1330
PluTI	GGCGCC	1 cut(s)	309
PmaCI	CACGTG	1 cut(s)	1778
PmlI	CACGTG	1 cut(s)	1778
PpsI	GAGTC	1 cut(s)	1330
Ppu21I	YACGTR	2 cut(s)	646, 1778
PscI	ACATGT	2 cut(s)	1024, 2169
PsiI	TTATAA	1 cut(s)	1563
Psp124BI	GAGCTC	1 cut(s)	2103
PspCI	CACGTG	1 cut(s)	1778
PspFI	CCCAGC	2 cut(s)	1088, 1798
PspN4I	GGNNCC	6 cut(s)	7, 271, 307, 1524, 1813, 1936
PspPI	GGNCC	2 cut(s)	538, 1726
PsuI	RGATCY	5 cut(s)	269, 1522, 1739, 1908, 2267
PvuII	CAGCTG	2 cut(s)	806, 1115
RsaI	GTAC	7 cut(s)	53, 137, 927, 1094, 1320, 1936, 2350
RsaNI	GTAC	7 cut(s)	52, 136, 926, 1093, 1319, 1935, 2349
RseI	CAYNNNNRTG	2 cut(s)	461, 1053
SacI	GAGCTC	1 cut(s)	2103
SapI	GCTCTTC	1 cut(s)	2108
SaqAI	TTAA	6 cut(s)	116, 390, 828, 1308, 2082, 2464
SatI	GCNGC	5 cut(s)	33, 1046, 1116, 1629, 1647
Sau3AI	GATC	6 cut(s)	269, 1522, 1708, 1739, 1908, 2267
Sau96I	GGNCC	2 cut(s)	538, 1726
SchI	GAGTC	1 cut(s)	1330
SduI	GDGCHC	8 cut(s)	8, 148, 261, 776, 940, 1756, 1850, 2103
SexAI	ACCWGGT	2 cut(s)	207, 1762
SfaNI	GCATC	7 cut(s)	772, 1057, 1246, 1421, 1912, 1923, 1934
SfcI	CTRYAG	1 cut(s)	696
SfoI	GGCGCC	1 cut(s)	307
Sfr274I	CTCGAG	1 cut(s)	593
SinI	GGWCC	1 cut(s)	1726
SlaI	CTCGAG	1 cut(s)	593
SmiMI	CAYNNNNRTG	2 cut(s)	461, 1053
SmlI	CTYRAG	4 cut(s)	593, 1394, 1823, 2120
SmoI	CTYRAG	4 cut(s)	593, 1394, 1823, 2120
SphI	GCATGC	2 cut(s)	653, 1082
Sse9I	AATT	6 cut(s)	452, 891, 980, 1255, 1757, 1983
SseBI	AGGCCT	2 cut(s)	1017, 2160
SsiI	CCGC	7 cut(s)	30, 33, 312, 367, 571, 1631, 1912
SspDI	GGCGCC	1 cut(s)	305
SspMI	CTAG	1 cut(s)	2447
SstI	GAGCTC	1 cut(s)	2103
StuI	AGGCCT	2 cut(s)	1017, 2160
StyI	CCWWGG	4 cut(s)	471, 559, 906, 1576
TaaI	ACNGT	8 cut(s)	25, 175, 517, 942, 1223, 1874, 2014, 2353
TaiI	ACGT	2 cut(s)	648, 1780
TaqI	TCGA	3 cut(s)	594, 756, 1707
TasI	AATT	6 cut(s)	452, 891, 980, 1255, 1757, 1983
TatI	WGTACW	3 cut(s)	135, 925, 1318
TauI	GCSGC	1 cut(s)	35
TfiI	GAWTC	3 cut(s)	497, 605, 1540
Tru1I	TTAA	6 cut(s)	116, 390, 828, 1308, 2082, 2464
Tru9I	TTAA	6 cut(s)	116, 390, 828, 1308, 2082, 2464
TseFI	GTSAC	3 cut(s)	175, 799, 2074
TseI	GCWGC	4 cut(s)	1045, 1115, 1628, 1646
Tsp45I	GTSAC	3 cut(s)	175, 799, 2074
TspGWI	ACGGA	5 cut(s)	234, 536, 1443, 1739, 2384
VneI	GTGCAC	1 cut(s)	772
VpaK11BI	GGWCC	1 cut(s)	1726
XapI	RAATTY	1 cut(s)	980
XceI	RCATGY	7 cut(s)	653, 712, 1028, 1082, 1447, 2173, 2201
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2188
XhoI	CTCGAG	1 cut(s)	593
XmnI	GAANNNNTTC	1 cut(s)	1250
XspI	CTAG	1 cut(s)	2447
Zsp2I	ATGCAT	2 cut(s)	1436, 1927

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.