MD10G1341400.v1.1

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000881
Physical Location & Seq
Forward (+)
41643565 .. 41645647
2083 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1341400.v1.1.491

Sequence Viewer

Length: 798 bp
ATGCTCCAGACCACCGTCTTTTTTCCGTCTTCCAGAGCCCTTCCACTCAACATCAACCACAGGATCATCTTCTCACGCGGACGGGGATGTGGATGGGGACGGGGGTCTGTGCGTCCTAACAGCAGCACTAGTGGAAGTAGTAACACTGCATCCCCATGGAGTACAAAACCTGTCTTTGAAGATGGTATTCTCAGTGTCGATGGTAACGATGTGTTGACACACGTGCCTGACAATGTGGTCGTCACACCATTGACCGACTCATCCGCATTCGTCGGTGCCACTTCTCAAACTGCTTCTTCACGTCATGTTTTTAAGCTTGGACTCGTACGGGATGTCAGATTGCTTAGTCTTTTTAGATTTAAACTTTGGTGGATGATACCAAGAGTGGGCAATACAGGAAGTGACATCCCAGTTGAAACTCAGATGTTGCTCCTGCAAGCAAAGTCAGCCGAAGAAGCTACTCCTCCTAATTATATCTTATTCTTGCCAGTTCTAGATGGTGAATTTAGAAGCAGCTTGCAGGGGAATTCATCGAATGAACTTGAGTTTTGTGTCGAAAGTGGTGACCCAGCTATCGTTACCTCCCAATCCCCAAAAGCAGTTTTTGTGAATTGCGGGAACCATCCTTTCGATCTGATGAAGGAATCTATGAAGATTTTGGAGAAGCATTTTGGAACTTTCTCTCTTAGAGAATCCAAACAGATGCCTGGAATGCTAGATTGCTTTGGCTGGTGTACCTGGGATGCCTTTTATCAAGGGGTTAATCCTCAAGGAATCAGAGATGGCCTGAAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

266

Amino Acids

29.22

Weight (kDa)

7.67

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 61 - 264 1.1e-68 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 236
AccB1I GGYRCC 1 cut(s) 275
AccII CGCG 1 cut(s) 78
AciI CCGC 3 cut(s) 78, 264, 615
AclWI GGATC 1 cut(s) 71
AcsI RAATTY 2 cut(s) 503, 526
AcvI CACGTG 1 cut(s) 223
AfaI GTAC 3 cut(s) 163, 327, 736
AfiI CCNNNNNNNGG 1 cut(s) 386
AflIII ACRYGT 1 cut(s) 220
AgsI TTSAA 2 cut(s) 179, 416
AhlI ACTAGT 1 cut(s) 128
AjiI CACGTC 1 cut(s) 302
AjnI CCWGG 2 cut(s) 706, 737
AluBI AGCT 4 cut(s) 316, 458, 516, 572
AluI AGCT 4 cut(s) 316, 458, 516, 572
AlwI GGATC 1 cut(s) 71
AoxI GGCC 1 cut(s) 784
ApeKI GCWGC 2 cut(s) 123, 513
ApoI RAATTY 2 cut(s) 503, 526
AsuHPI GGTGA 2 cut(s) 512, 575
BanI GGYRCC 1 cut(s) 275
BanII GRGCYC 1 cut(s) 40
BbrPI CACGTG 1 cut(s) 223
BbsI GAAGAC 1 cut(s) 21
BbvI GCAGC 2 cut(s) 135, 525
BccI CCATC 6 cut(s) 87, 176, 194, 491, 630, 776
BciT130I CCWGG 2 cut(s) 708, 739
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 3 cut(s) 129, 494, 716
BisI GCNGC 2 cut(s) 124, 514
BlsI GCNGC 2 cut(s) 125, 515
Bme1390I CCNGG 2 cut(s) 708, 739
BmgBI CACGTC 1 cut(s) 302
BmiI GGNNCC 2 cut(s) 277, 620
BmrFI CCNGG 2 cut(s) 708, 739
BmrI ACTGGG 1 cut(s) 404
BmsI GCATC 3 cut(s) 158, 693, 733
BmuI ACTGGG 1 cut(s) 404
BoxI GACNNNNGTC 2 cut(s) 14, 103
BpiI GAAGAC 1 cut(s) 21
BpuEI CTTGAG 2 cut(s) 563, 753
BsaAI YACGTR 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 155, 738
Bsc4I CCNNNNNNNGG 1 cut(s) 386
Bse1I ACTGG 2 cut(s) 410, 488
BseBI CCWGG 2 cut(s) 708, 739
BseDI CCNNGG 2 cut(s) 155, 738
BseGI GGATG 9 cut(s) 92, 98, 149, 260, 337, 378, 405, 622, 748
BseLI CCNNNNNNNGG 1 cut(s) 386
BseMII CTCAG 2 cut(s) 205, 434
BseNI ACTGG 2 cut(s) 410, 488
BseRI GAGGAG 1 cut(s) 453
BseXI GCAGC 2 cut(s) 135, 525
BseYI CCCAGC 1 cut(s) 568
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 1 cut(s) 786
BshNI GGYRCC 1 cut(s) 275
BsiWI CGTACG 1 cut(s) 325
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 111
BsmI GAATGC 2 cut(s) 266, 717
BsnI GGCC 1 cut(s) 786
Bsp1286I GDGCHC 1 cut(s) 40
Bsp143I GATC 2 cut(s) 63, 631
Bsp19I CCATGG 1 cut(s) 155
BspACI CCGC 3 cut(s) 78, 264, 615
BspANI GGCC 1 cut(s) 786
BspCNI CTCAG 2 cut(s) 204, 433
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 2 cut(s) 277, 620
BspPI GGATC 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 275
BsrI ACTGG 2 cut(s) 410, 488
BssECI CCNNGG 2 cut(s) 155, 738
BssMI GATC 2 cut(s) 63, 631
BssT1I CCWWGG 1 cut(s) 155
Bst2UI CCWGG 2 cut(s) 708, 739
Bst4CI ACNGT 1 cut(s) 16
BstBAI YACGTR 1 cut(s) 223
BstC8I GCNNGC 2 cut(s) 438, 518
BstDEI CTNAG 4 cut(s) 191, 344, 420, 686
BstDSI CCRYGG 1 cut(s) 155
BstEII GGTNACC 1 cut(s) 563
BstF5I GGATG 9 cut(s) 92, 98, 149, 260, 337, 378, 405, 622, 748
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 2 cut(s) 66, 634
BstMBI GATC 2 cut(s) 63, 631
BstMWI GCNNNNNNNGC 3 cut(s) 446, 455, 712
BstNI CCWGG 2 cut(s) 708, 739
BstPAI GACNNNNGTC 2 cut(s) 14, 103
BstPI GGTNACC 1 cut(s) 563
BstSCI CCNGG 2 cut(s) 706, 737
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 2 cut(s) 135, 525
BstV2I GAAGAC 1 cut(s) 21
BsuRI GGCC 1 cut(s) 786
BtgI CCRYGG 1 cut(s) 155
BtrI CACGTC 1 cut(s) 302
BtsCI GGATG 9 cut(s) 92, 98, 149, 260, 337, 378, 405, 622, 748
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 2 cut(s) 144, 199
Cac8I GCNNGC 2 cut(s) 438, 518
CseI GACGC 1 cut(s) 101
Csp6I GTAC 3 cut(s) 162, 326, 735
CviAII CATG 2 cut(s) 156, 305
CviJI RGCY 8 cut(s) 38, 316, 449, 458, 516, 572, 729, 786
CviKI_1 RGCY 8 cut(s) 38, 316, 449, 458, 516, 572, 729, 786
CviQI GTAC 3 cut(s) 162, 326, 735
DdeI CTNAG 4 cut(s) 191, 344, 420, 686
DpnI GATC 2 cut(s) 65, 633
DpnII GATC 2 cut(s) 63, 631
DraI TTTAAA 1 cut(s) 361
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
Eco130I CCWWGG 1 cut(s) 155
Eco24I GRGCYC 1 cut(s) 40
Eco72I CACGTG 1 cut(s) 223
Eco91I GGTNACC 1 cut(s) 563
EcoO65I GGTNACC 1 cut(s) 563
EcoRI GAATTC 1 cut(s) 526
EcoRII CCWGG 2 cut(s) 706, 737
EcoT14I CCWWGG 1 cut(s) 155
EcoT38I GRGCYC 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 155
FaeI CATG 2 cut(s) 159, 308
FaiI YATR 4 cut(s) 157, 306, 474, 650
FaqI GGGAC 1 cut(s) 111
FatI CATG 2 cut(s) 155, 304
FauI CCCGC 1 cut(s) 608
Fnu4HI GCNGC 2 cut(s) 124, 514
FokI GGATG 9 cut(s) 99, 105, 136, 247, 344, 385, 392, 609, 755
FriOI GRGCYC 1 cut(s) 40
Fsp4HI GCNGC 2 cut(s) 124, 514
FspBI CTAG 3 cut(s) 129, 494, 716
GluI GCNGC 2 cut(s) 124, 514
GsaI CCCAGC 1 cut(s) 572
HaeIII GGCC 1 cut(s) 786
HgaI GACGC 1 cut(s) 101
Hin1II CATG 2 cut(s) 159, 308
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 5 cut(s) 257, 321, 644, 692, 774
HphI GGTGA 2 cut(s) 512, 575
Hpy166II GTNNAC 2 cut(s) 216, 735
Hpy188I TCNGA 4 cut(s) 338, 423, 636, 779
Hpy188III TCNNGA 3 cut(s) 7, 33, 494
Hpy8I GTNNAC 2 cut(s) 216, 735
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 2 cut(s) 50, 634
HpyCH4III ACNGT 1 cut(s) 16
HpyCH4IV ACGT 2 cut(s) 222, 301
HpyCH4V TGCA 3 cut(s) 149, 436, 520
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 455, 712
HpyF3I CTNAG 4 cut(s) 191, 344, 420, 686
HpySE526I ACGT 2 cut(s) 222, 301
Hsp92II CATG 2 cut(s) 159, 308
Kzo9I GATC 2 cut(s) 63, 631
LmnI GCTCC 2 cut(s) 9, 435
Lsp1109I GCAGC 2 cut(s) 135, 525
LweI GCATC 3 cut(s) 158, 693, 733
MaeI CTAG 3 cut(s) 129, 494, 716
MaeII ACGT 2 cut(s) 222, 301
MaeIII GTNAC 6 cut(s) 140, 203, 241, 401, 563, 577
MalI GATC 2 cut(s) 65, 633
MboI GATC 2 cut(s) 63, 631
MboII GAAGA 6 cut(s) 21, 61, 191, 288, 464, 664
MhlI GDGCHC 1 cut(s) 40
MluCI AATT 4 cut(s) 469, 503, 526, 610
MlyI GAGTC 2 cut(s) 251, 315
MnlI CCTC 3 cut(s) 474, 592, 777
MseI TTAA 3 cut(s) 312, 360, 762
MslI CAYNNNNRTG 1 cut(s) 154
MspR9I CCNGG 2 cut(s) 708, 739
Mva1269I GAATGC 2 cut(s) 266, 717
MvaI CCWGG 2 cut(s) 708, 739
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 3 cut(s) 446, 455, 712
NcoI CCATGG 1 cut(s) 155
NdeII GATC 2 cut(s) 63, 631
NlaIII CATG 2 cut(s) 159, 308
NlaIV GGNNCC 2 cut(s) 277, 620
NmuCI GTSAC 3 cut(s) 241, 401, 563
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PctI GAATGC 2 cut(s) 266, 717
PfeI GAWTC 3 cut(s) 644, 692, 774
Pfl23II CGTACG 1 cut(s) 325
PkrI GCNGC 2 cut(s) 125, 515
PleI GAGTC 2 cut(s) 251, 315
PmaCI CACGTG 1 cut(s) 223
PmlI CACGTG 1 cut(s) 223
PpsI GAGTC 2 cut(s) 251, 315
Ppu21I YACGTR 1 cut(s) 223
PshAI GACNNNNGTC 2 cut(s) 14, 103
Psp6I CCWGG 2 cut(s) 706, 737
PspCI CACGTG 1 cut(s) 223
PspEI GGTNACC 1 cut(s) 563
PspFI CCCAGC 1 cut(s) 568
PspGI CCWGG 2 cut(s) 706, 737
PspLI CGTACG 1 cut(s) 325
PspN4I GGNNCC 2 cut(s) 277, 620
RsaI GTAC 3 cut(s) 163, 327, 736
RsaNI GTAC 3 cut(s) 162, 326, 735
RseI CAYNNNNRTG 1 cut(s) 154
SaqAI TTAA 3 cut(s) 312, 360, 762
SatI GCNGC 2 cut(s) 124, 514
Sau3AI GATC 2 cut(s) 63, 631
SchI GAGTC 2 cut(s) 251, 315
ScrFI CCNGG 2 cut(s) 708, 739
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 3 cut(s) 158, 693, 733
SmiMI CAYNNNNRTG 1 cut(s) 154
SmlI CTYRAG 2 cut(s) 542, 768
SmoI CTYRAG 2 cut(s) 542, 768
SpeI ACTAGT 1 cut(s) 128
Sse9I AATT 4 cut(s) 469, 503, 526, 610
SsiI CCGC 3 cut(s) 78, 264, 615
SspMI CTAG 3 cut(s) 129, 494, 716
StyD4I CCNGG 2 cut(s) 706, 737
StyI CCWWGG 1 cut(s) 155
TaaI ACNGT 1 cut(s) 16
TaiI ACGT 2 cut(s) 225, 304
TaqI TCGA 4 cut(s) 198, 533, 555, 630
TaqII GACCGA 1 cut(s) 269
TasI AATT 4 cut(s) 469, 503, 526, 610
TatI WGTACW 1 cut(s) 161
TfiI GAWTC 3 cut(s) 644, 692, 774
Tru1I TTAA 3 cut(s) 312, 360, 762
Tru9I TTAA 3 cut(s) 312, 360, 762
TscAI CASTG 2 cut(s) 151, 199
TseFI GTSAC 3 cut(s) 241, 401, 563
TseI GCWGC 2 cut(s) 123, 513
Tsp45I GTSAC 3 cut(s) 241, 401, 563
TspDTI ATGAA 4 cut(s) 519, 552, 653, 665
TspGWI ACGGA 1 cut(s) 15
TspRI CASTG 2 cut(s) 151, 199
XapI RAATTY 2 cut(s) 503, 526
XbaI TCTAGA 1 cut(s) 493
XspI CTAG 3 cut(s) 129, 494, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.