RLG00000023385

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000881
Physical Location & Seq
Reverse (-)
22904669 .. 22909994
5326 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023385

Sequence Viewer

Length: 2424 bp
ATGGAATATAACGTGGCAACATCTGGAAGAATCGAGGATGGAAGTTGCTCGAAGTTTAACACGAGGGTTAAGAAAGCTCTGCCTTCTGTAGTACTTGGGTGCTCTCTCAAGTCTCAGTCAACCATGACTGTCACTCCAAACATTTCCATCAACGATGGAAACCTGGTGGTTCACGGAAAGACCATATTGACCGGAGTGCCGGAAAACATTGTGCTCACTCCAGGGTCCGGTGTCGGCCTCGTTGCTGGTGCTTTCATTGGCGCCACCGCCTCTCACAGCAAAAGCCTCCATGTCTTCCCTGTCGGTGTTTTAGAGGGTCAGCGGTTCATGTGCCTTTTCCGGTTTAAGCTGTGGTGGATGACGCAGAGGATGGGGTCATGTGGGAAAGAAGTTCCACTGGAGACCCAATTCATGCTTGTGGAGACCAAGGATGATGGTGAAGGAAGTGATGGATGCTCTTCTACCATTTACACCGTCTTCCTTCCTCTGCTGGAGGGCCAGTTCCGTTCGGTTCTGCAAGGGAACGAGCGGAATGAAGTGGAGATATGCCTCGAGAGTGGAGATTCTGATGTTCAGACCAATCAAGGTCTTTGCCTTGTTTACGTGCATGCTGGAACTAATCCATTTGAAGTTATCAACCAGGCTGTAAAGGCTGTAGAAAAACACATGCAAACCTTTGTCCATCGTGAGAAGAAAAAGTTGCCTTCTTTCCTCGACTGGTTTGGCTGGTGCACATGGGATGCTTTCTATACCGATGTCACAGCTGAGGGTGTCGTGGATGGTCTTAAAAGCTTGAATGAGGGAGGGACGCCTCCGCGATTTCTAATCGTAGATGATGGTTGGCAACAAATTGAAAGTAAACCCAAGGATACTAATGTTGCAGTACAAGAAGGAGCACAGTTTGCAAGTAGGCTTACAGGAATAAAAGAGAATGAAAAATTCCAAAAGAATGGACAGAGTGAGCAAGTCTCAGGCCTGAAACATGTTGTTGATGAAGCGAAGCAGCATCACAATGTGAAGTTTGTGTATGTATGGCATGCCTTAATTGGGTACTGGGGTGGAGTGAAACCAGCTGCTTCTGGCATGGAGCATTATGACACTGCTTTGGCATATCCAGTCTCATCTCCTGGTGTGATGGGAAACCAACCTGACATAGTTTTTGACAGCTTATCTGTTCACGGTCTTGGTTTGGTGCATCCAAAGAAAGTTTTCAATTTCTACAATGAGCTTCATGCGTATTTGGCTTCCTGTGGAGTGGATGGAGTTAAGGTTGATGTACAGAACATCATTGAGACTCTTGGGTCTGGTCATGGTGGCAGAGTTTCTCTGACCCGAAGCTATCACCAGGCACTTGAGGCTTCGGTTGCTCGGAACTTTCCTGACAACGGATGCATTTCTTGCATGTGTCATAACACTGATGGGCTATATAGTTCCAAGCAGACTGCTGTTGTGAGAGCTTCTGATGACTTCTACCCACGGGATCCTGCTTCTCACACGATTCACATATCTTCTGTGGCTTATAACACCCTTTTCCTTGGCGAGTTTATGCAACCAGACTGGGATATGTTTCATAGTTTACATCCAGCAGCGGATTATCATGGTGCAGCTCGTTCTATTGGTGGATGTGCAATCTATGTCAGTGATAAACCTGGAAACCACAACTTTGATCTTTTGAGGAAGCTGGTCCTGCCTGATGGATCTGTCCTCCGTGCTCAATTACCTGGTAGGCCAACAAGTGATTGTCTCTTTGCTGACCCAGCAAGAGATGGAACCAGCTTGCTCAAGATATGGAACGTGAACAAGTGCTCTGGTGTTGTTGGTGTATTCAACTGTCAAGGGGCTGGTTGGTGCAAGGTTGAAAAGAAGACCCGCATCCATGATGCATCTCCTGGTACCCTCACTGGTTCTGTTCGTGCTATTGATGTTGATAGCCTTGCCCAAATTGTTGGTGACGATTGGAATGGAGAAACAGTAGTTTATGCCCATAAGTCAGGAGAGGTGTTTCGTTTACCAAAAGATGTTTCAGTGCCAGTGACACTTAAAGTTCTGGAGTATGAGCTCTTCCATTTCTGTCCTCTCAAGGAGGTTACACCCAACATCTCATTTGCTCCAATAGGCCTGCTTGACATGTTCAACTCCAGTGGTGCTGTGGAGCATGTTGTAATCCATCTGGCTTCTGACGGCGAAGTTTCTTCAGAGCTTACCACTTCTGTCAGCGAGACTAGATCTTCAACTGCCACAATCACCATCAAAACCAGAGGATGTGGAAGGTTTGGAGCTTACTCTTCTCAGTGTCCCCTGAAGTGTACCGTTGATAGAGTTGAGACGGACTTTGAATATGACCCTGCAACTGGATTACTAACCTTTACCATCCCTATCCCAAAAGAGGATATGTATAAATGGCTAGTAGAAATCCAAGTTTAA

Protein Analysis

808

Amino Acids

88.16

Weight (kDa)

5.82

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 48 - 790 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1521
AasI GACNNNNNNGTC 1 cut(s) 1300
Acc65I GGTACC 1 cut(s) 1892
AccB1I GGYRCC 2 cut(s) 260, 1892
AccBSI CCGCTC 1 cut(s) 529
AccII CGCG 1 cut(s) 817
AciI CCGC 6 cut(s) 267, 322, 529, 815, 1589, 1870
AclWI GGATC 3 cut(s) 1475, 1488, 1705
AcsI RAATTY 1 cut(s) 938
AcuI CTGAAG 2 cut(s) 2178, 2321
AcyI GRCGYC 2 cut(s) 261, 809
AdeI CACNNNGTG 1 cut(s) 1015
AfaI GTAC 6 cut(s) 93, 885, 1052, 1278, 1894, 2308
AfiI CCNNNNNNNGG 5 cut(s) 227, 1047, 1385, 2351, 2386
AflIII ACRYGT 2 cut(s) 982, 2127
AgsI TTSAA 9 cut(s) 629, 796, 854, 1213, 1828, 1859, 2134, 2232, 2336
AjnI CCWGG 8 cut(s) 162, 220, 639, 1126, 1344, 1648, 1720, 1888
AjuI GAANNNNNNNTTGG 2 cut(s) 2005, 2037
AloI GAACNNNNNNTCC 2 cut(s) 485, 517
Alw21I GWGCWC 7 cut(s) 104, 216, 734, 898, 1714, 1808, 2061
Alw26I GTCTC 9 cut(s) 117, 395, 416, 973, 1123, 1286, 1748, 2213, 2318
Alw44I GTGCAC 1 cut(s) 730
AlwI GGATC 3 cut(s) 1475, 1488, 1705
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 5 cut(s) 235, 496, 973, 1727, 2116
ApaLI GTGCAC 1 cut(s) 730
ApeKI GCWGC 4 cut(s) 1003, 1073, 1586, 1604
ApoI RAATTY 1 cut(s) 938
ArsI GACNNNNNNTTYG 2 cut(s) 1142, 1174
Asp700I GAANNNNTTC 1 cut(s) 1208
Asp718I GGTACC 1 cut(s) 1892
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 3 cut(s) 225, 496, 1684
AsuHPI GGTGA 4 cut(s) 449, 1334, 1961, 2236
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 2 cut(s) 225, 1684
BaeGI GKGCMC 1 cut(s) 734
BaeI ACNNNNGTAYC 4 cut(s) 861, 894, 1042, 1075
BamHI GGATCC 1 cut(s) 1480
BanI GGYRCC 2 cut(s) 260, 1892
BanII GRGCYC 1 cut(s) 2061
BarI GAAGNNNNNNTAC 2 cut(s) 2045, 2077
BauI CACGAG 1 cut(s) 61
BbsI GAAGAC 3 cut(s) 286, 469, 1871
Bbv12I GWGCWC 7 cut(s) 104, 216, 734, 898, 1714, 1808, 2061
BbvCI CCTCAGC 1 cut(s) 765
BbvI GCAGC 4 cut(s) 1015, 1060, 1598, 1616
BceAI ACGGC 1 cut(s) 2197
BciT130I CCWGG 8 cut(s) 164, 222, 641, 1128, 1346, 1650, 1722, 1890
BciVI GTATCC 1 cut(s) 862
BcoDI GTCTC 9 cut(s) 117, 395, 416, 973, 1123, 1286, 1748, 2213, 2318
BfaI CTAG 2 cut(s) 2223, 2405
BfmI CTRYAG 2 cut(s) 87, 654
BfoI RGCGCY 1 cut(s) 264
BfuI GTATCC 1 cut(s) 862
BglII AGATCT 1 cut(s) 2225
BisI GCNGC 4 cut(s) 1004, 1074, 1587, 1605
BlsI GCNGC 4 cut(s) 1005, 1075, 1588, 1606
BmcAI AGTACT 1 cut(s) 93
Bme1390I CCNGG 8 cut(s) 164, 222, 641, 1128, 1346, 1650, 1722, 1890
Bme18I GGWCC 2 cut(s) 225, 1684
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 3 cut(s) 225, 496, 1684
BmiI GGNNCC 5 cut(s) 226, 262, 1482, 1771, 1894
BmrFI CCNGG 8 cut(s) 164, 222, 641, 1128, 1346, 1650, 1722, 1890
BmrI ACTGGG 2 cut(s) 1063, 1567
BmsI GCATC 8 cut(s) 443, 730, 1015, 1204, 1379, 1870, 1881, 1892
BmuI ACTGGG 2 cut(s) 1063, 1567
BpiI GAAGAC 3 cut(s) 286, 469, 1871
BplI GAGNNNNNCTC 2 cut(s) 953, 985
BpmI CTGGAG 5 cut(s) 204, 419, 512, 2069, 2122
Bpu10I CCTNAGC 1 cut(s) 765
BpuEI CTTGAG 4 cut(s) 92, 1373, 1766, 2063
BsaAI YACGTR 1 cut(s) 604
BsaHI GRCGYC 2 cut(s) 261, 809
BsaI GGTCTC 2 cut(s) 395, 416
BsaJI CCNNGG 5 cut(s) 221, 426, 864, 1475, 1534
BsaWI WCCGGW 3 cut(s) 191, 227, 339
BsaXI ACNNNNNCTCC 8 cut(s) 118, 148, 485, 515, 1254, 1284, 1956, 1986
Bsc4I CCNNNNNNNGG 5 cut(s) 227, 1047, 1385, 2351, 2386
BseBI CCWGG 8 cut(s) 164, 222, 641, 1128, 1346, 1650, 1722, 1890
BseDI CCNNGG 5 cut(s) 221, 426, 864, 1475, 1534
BseLI CCNNNNNNNGG 5 cut(s) 227, 1047, 1385, 2351, 2386
BseMII CTCAG 4 cut(s) 128, 756, 984, 2303
BseSI GKGCMC 1 cut(s) 734
BseXI GCAGC 4 cut(s) 1015, 1060, 1598, 1616
BseYI CCCAGC 1 cut(s) 1756
BsgI GTGCAG 1 cut(s) 1623
Bsh1236I CGCG 1 cut(s) 817
BshFI GGCC 5 cut(s) 237, 498, 975, 1729, 2118
BshNI GGYRCC 2 cut(s) 260, 1892
BsiHKAI GWGCWC 7 cut(s) 104, 216, 734, 898, 1714, 1808, 2061
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 4 cut(s) 192, 200, 228, 340
BslFI GGGAC 2 cut(s) 820, 2280
BslI CCNNNNNNNGG 5 cut(s) 227, 1047, 1385, 2351, 2386
BsmAI GTCTC 9 cut(s) 117, 395, 416, 973, 1123, 1286, 1748, 2213, 2318
BsmBI CGTCTC 1 cut(s) 2318
BsmFI GGGAC 2 cut(s) 820, 2280
BsnI GGCC 5 cut(s) 237, 498, 975, 1729, 2118
Bso31I GGTCTC 2 cut(s) 395, 416
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 7 cut(s) 104, 216, 734, 898, 1714, 1808, 2061
Bsp1407I TGTACA 1 cut(s) 1276
Bsp143I GATC 4 cut(s) 1480, 1666, 1697, 2225
BspACI CCGC 6 cut(s) 267, 322, 529, 815, 1589, 1870
BspANI GGCC 5 cut(s) 237, 498, 975, 1729, 2118
BspCNI CTCAG 4 cut(s) 127, 757, 983, 2302
BspFNI CGCG 1 cut(s) 817
BspLI GGNNCC 5 cut(s) 226, 262, 1482, 1771, 1894
BspPI GGATC 3 cut(s) 1475, 1488, 1705
BspQI GCTCTTC 2 cut(s) 463, 2066
BspT107I GGYRCC 2 cut(s) 260, 1892
BspTNI GGTCTC 2 cut(s) 395, 416
BsrBI CCGCTC 1 cut(s) 529
BsrGI TGTACA 1 cut(s) 1276
BssECI CCNNGG 5 cut(s) 221, 426, 864, 1475, 1534
BssMI GATC 4 cut(s) 1480, 1666, 1697, 2225
BssNI GRCGYC 2 cut(s) 261, 809
BssSI CACGAG 1 cut(s) 61
BssT1I CCWWGG 3 cut(s) 426, 864, 1534
Bst2BI CACGAG 1 cut(s) 61
Bst2UI CCWGG 8 cut(s) 164, 222, 641, 1128, 1346, 1650, 1722, 1890
Bst4CI ACNGT 7 cut(s) 130, 475, 900, 1181, 1832, 1972, 2311
Bst6I CTCTTC 3 cut(s) 463, 2066, 2290
BstACI GRCGYC 2 cut(s) 261, 809
BstAPI GCANNNNNTGC 2 cut(s) 902, 1398
BstAUI TGTACA 1 cut(s) 1276
BstBAI YACGTR 1 cut(s) 604
BstC8I GCNNGC 4 cut(s) 609, 1038, 1778, 2120
BstDEI CTNAG 4 cut(s) 114, 765, 970, 2289
BstDSI CCRYGG 1 cut(s) 1475
BstFNI CGCG 1 cut(s) 817
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 4 cut(s) 1483, 1669, 1700, 2228
BstMAI GTCTC 9 cut(s) 117, 395, 416, 973, 1123, 1286, 1748, 2213, 2318
BstMBI GATC 4 cut(s) 1480, 1666, 1697, 2225
BstMWI GCNNNNNNNGC 8 cut(s) 650, 902, 1241, 1355, 1364, 1398, 1687, 1757
BstNI CCWGG 8 cut(s) 164, 222, 641, 1128, 1346, 1650, 1722, 1890
BstNSI RCATGY 7 cut(s) 611, 670, 986, 1040, 1405, 2131, 2159
BstSCI CCNGG 8 cut(s) 162, 220, 639, 1126, 1344, 1648, 1720, 1888
BstSFI CTRYAG 2 cut(s) 87, 654
BstSLI GKGCMC 1 cut(s) 734
BstUI CGCG 1 cut(s) 817
BstV1I GCAGC 4 cut(s) 1015, 1060, 1598, 1616
BstV2I GAAGAC 3 cut(s) 286, 469, 1871
BstX2I RGATCY 3 cut(s) 1480, 1697, 2225
BstXI CCANNNNNNTGG 2 cut(s) 950, 1946
BstYI RGATCY 3 cut(s) 1480, 1697, 2225
BsuI GTATCC 1 cut(s) 862
BsuRI GGCC 5 cut(s) 237, 498, 975, 1729, 2118
BtgI CCRYGG 1 cut(s) 1475
BtsI GCAGTG 1 cut(s) 1098
BtsIMutI CAGTG 9 cut(s) 395, 1098, 1413, 1645, 1899, 2031, 2037, 2146, 2297
Cac8I GCNNGC 4 cut(s) 609, 1038, 1778, 2120
CfoI GCGC 1 cut(s) 263
Cfr13I GGNCC 3 cut(s) 225, 496, 1684
CseI GACGC 2 cut(s) 370, 817
CsiI ACCWGGT 2 cut(s) 162, 1720
Csp6I GTAC 6 cut(s) 92, 884, 1051, 1277, 1893, 2307
CspCI CAANNNNNGTGG 2 cut(s) 2122, 2157
CviQI GTAC 6 cut(s) 92, 884, 1051, 1277, 1893, 2307
DdeI CTNAG 4 cut(s) 114, 765, 970, 2289
DinI GGCGCC 1 cut(s) 262
DpnI GATC 4 cut(s) 1482, 1668, 1699, 2227
DpnII GATC 4 cut(s) 1480, 1666, 1697, 2225
DraIII CACNNNGTG 1 cut(s) 1015
DrdI GACNNNNNNGTC 1 cut(s) 1300
DseDI GACNNNNNNGTC 1 cut(s) 1300
Eam1104I CTCTTC 3 cut(s) 463, 2066, 2290
EarI CTCTTC 3 cut(s) 463, 2066, 2290
Ecl136II GAGCTC 1 cut(s) 2059
Eco130I CCWWGG 3 cut(s) 426, 864, 1534
Eco147I AGGCCT 2 cut(s) 975, 2118
Eco24I GRGCYC 1 cut(s) 2061
Eco31I GGTCTC 2 cut(s) 395, 416
Eco47I GGWCC 2 cut(s) 225, 1684
Eco53kI GAGCTC 1 cut(s) 2059
Eco57I CTGAAG 2 cut(s) 2178, 2321
Eco88I CYCGRG 1 cut(s) 551
EcoICRI GAGCTC 1 cut(s) 2059
EcoRII CCWGG 8 cut(s) 162, 220, 639, 1126, 1344, 1648, 1720, 1888
EcoT14I CCWWGG 3 cut(s) 426, 864, 1534
EcoT22I ATGCAT 2 cut(s) 1394, 1885
EcoT38I GRGCYC 1 cut(s) 2061
EgeI GGCGCC 1 cut(s) 262
EheI GGCGCC 1 cut(s) 262
ErhI CCWWGG 3 cut(s) 426, 864, 1534
Esp3I CGTCTC 1 cut(s) 2318
FaqI GGGAC 2 cut(s) 820, 2280
FauI CCCGC 1 cut(s) 1877
Fnu4HI GCNGC 4 cut(s) 1004, 1074, 1587, 1605
FriOI GRGCYC 1 cut(s) 2061
Fsp4HI GCNGC 4 cut(s) 1004, 1074, 1587, 1605
FspBI CTAG 2 cut(s) 2223, 2405
GlaI GCGC 1 cut(s) 262
GluI GCNGC 4 cut(s) 1004, 1074, 1587, 1605
GsaI CCCAGC 1 cut(s) 1760
GsuI CTGGAG 5 cut(s) 204, 419, 512, 2069, 2122
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 5 cut(s) 237, 498, 975, 1729, 2118
HapII CCGG 4 cut(s) 192, 200, 228, 340
HgaI GACGC 2 cut(s) 370, 817
HhaI GCGC 1 cut(s) 263
Hin1I GRCGYC 2 cut(s) 261, 809
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HincII GTYRAC 1 cut(s) 120
HindII GTYRAC 1 cut(s) 120
HindIII AAGCTT 1 cut(s) 790
HinfI GANTC 4 cut(s) 30, 563, 1294, 1498
HpaII CCGG 4 cut(s) 192, 200, 228, 340
HphI GGTGA 4 cut(s) 449, 1334, 1961, 2236
Hpy188I TCNGA 7 cut(s) 568, 576, 1329, 1371, 1462, 2179, 2197
Hpy188III TCNNGA 7 cut(s) 24, 553, 686, 1379, 1783, 1992, 2048
HpyAV CCTTC 6 cut(s) 93, 434, 491, 714, 884, 2262
HpyCH4III ACNGT 7 cut(s) 130, 475, 900, 1181, 1832, 1972, 2311
HpyCH4IV ACGT 3 cut(s) 12, 603, 1794
HpyF10VI GCNNNNNNNGC 8 cut(s) 650, 902, 1241, 1355, 1364, 1398, 1687, 1757
HpyF3I CTNAG 4 cut(s) 114, 765, 970, 2289
HpySE526I ACGT 3 cut(s) 12, 603, 1794
Hsp92I GRCGYC 2 cut(s) 261, 809
HspAI GCGC 1 cut(s) 261
KasI GGCGCC 1 cut(s) 260
KpnI GGTACC 1 cut(s) 1896
Kzo9I GATC 4 cut(s) 1480, 1666, 1697, 2225
LguI GCTCTTC 2 cut(s) 463, 2066
LmnI GCTCC 5 cut(s) 893, 1087, 2113, 2152, 2276
Lsp1109I GCAGC 4 cut(s) 1015, 1060, 1598, 1616
LweI GCATC 8 cut(s) 443, 730, 1015, 1204, 1379, 1870, 1881, 1892
MabI ACCWGGT 2 cut(s) 162, 1720
MaeI CTAG 2 cut(s) 2223, 2405
MaeII ACGT 3 cut(s) 12, 603, 1794
MaeIII GTNAC 5 cut(s) 130, 757, 1949, 2032, 2086
MalI GATC 4 cut(s) 1482, 1668, 1699, 2227
MbiI CCGCTC 1 cut(s) 529
MboI GATC 4 cut(s) 1480, 1666, 1697, 2225
MflI RGATCY 3 cut(s) 1480, 1697, 2225
MhlI GDGCHC 7 cut(s) 104, 216, 734, 898, 1714, 1808, 2061
MluCI AATT 7 cut(s) 407, 849, 938, 1044, 1213, 1715, 1941
Mly113I GGCGCC 1 cut(s) 261
MlyI GAGTC 1 cut(s) 1288
Mph1103I ATGCAT 2 cut(s) 1394, 1885
MroXI GAANNNNTTC 1 cut(s) 1208
MseI TTAA 8 cut(s) 57, 69, 345, 786, 1043, 1266, 2040, 2422
MslI CAYNNNNRTG 2 cut(s) 416, 1011
MspA1I CMGCKG 4 cut(s) 322, 764, 1073, 1589
MspI CCGG 4 cut(s) 192, 200, 228, 340
MspR9I CCNGG 8 cut(s) 164, 222, 641, 1128, 1346, 1650, 1722, 1890
MvaI CCWGG 8 cut(s) 164, 222, 641, 1128, 1346, 1650, 1722, 1890
MvnI CGCG 1 cut(s) 817
MwoI GCNNNNNNNGC 8 cut(s) 650, 902, 1241, 1355, 1364, 1398, 1687, 1757
NarI GGCGCC 1 cut(s) 261
NdeII GATC 4 cut(s) 1480, 1666, 1697, 2225
NlaIV GGNNCC 5 cut(s) 226, 262, 1482, 1771, 1894
NmuCI GTSAC 4 cut(s) 130, 757, 1949, 2032
NsiI ATGCAT 2 cut(s) 1394, 1885
NspI RCATGY 7 cut(s) 611, 670, 986, 1040, 1405, 2131, 2159
PaeI GCATGC 2 cut(s) 611, 1040
PaeR7I CTCGAG 1 cut(s) 551
PceI AGGCCT 2 cut(s) 975, 2118
PciI ACATGT 2 cut(s) 982, 2127
PciSI GCTCTTC 2 cut(s) 463, 2066
PdmI GAANNNNTTC 1 cut(s) 1208
PfeI GAWTC 3 cut(s) 30, 563, 1498
PkrI GCNGC 4 cut(s) 1005, 1075, 1588, 1606
PleI GAGTC 1 cut(s) 1288
PluTI GGCGCC 1 cut(s) 264
PpsI GAGTC 1 cut(s) 1288
Ppu21I YACGTR 1 cut(s) 604
PscI ACATGT 2 cut(s) 982, 2127
PsiI TTATAA 1 cut(s) 1521
Psp124BI GAGCTC 1 cut(s) 2061
Psp6I CCWGG 8 cut(s) 162, 220, 639, 1126, 1344, 1648, 1720, 1888
PspFI CCCAGC 1 cut(s) 1756
PspGI CCWGG 8 cut(s) 162, 220, 639, 1126, 1344, 1648, 1720, 1888
PspN4I GGNNCC 5 cut(s) 226, 262, 1482, 1771, 1894
PspPI GGNCC 3 cut(s) 225, 496, 1684
PsuI RGATCY 3 cut(s) 1480, 1697, 2225
PvuII CAGCTG 2 cut(s) 764, 1073
RsaI GTAC 6 cut(s) 93, 885, 1052, 1278, 1894, 2308
RsaNI GTAC 6 cut(s) 92, 884, 1051, 1277, 1893, 2307
RseI CAYNNNNRTG 2 cut(s) 416, 1011
SacI GAGCTC 1 cut(s) 2061
SapI GCTCTTC 2 cut(s) 463, 2066
SaqAI TTAA 8 cut(s) 57, 69, 345, 786, 1043, 1266, 2040, 2422
SatI GCNGC 4 cut(s) 1004, 1074, 1587, 1605
Sau3AI GATC 4 cut(s) 1480, 1666, 1697, 2225
Sau96I GGNCC 3 cut(s) 225, 496, 1684
ScaI AGTACT 1 cut(s) 93
SchI GAGTC 1 cut(s) 1288
ScrFI CCNGG 8 cut(s) 164, 222, 641, 1128, 1346, 1650, 1722, 1890
SduI GDGCHC 7 cut(s) 104, 216, 734, 898, 1714, 1808, 2061
SexAI ACCWGGT 2 cut(s) 162, 1720
SfaNI GCATC 8 cut(s) 443, 730, 1015, 1204, 1379, 1870, 1881, 1892
SfcI CTRYAG 2 cut(s) 87, 654
SfoI GGCGCC 1 cut(s) 262
Sfr274I CTCGAG 1 cut(s) 551
SinI GGWCC 2 cut(s) 225, 1684
SlaI CTCGAG 1 cut(s) 551
SmiMI CAYNNNNRTG 2 cut(s) 416, 1011
SmlI CTYRAG 5 cut(s) 107, 551, 1352, 1781, 2078
SmoI CTYRAG 5 cut(s) 107, 551, 1352, 1781, 2078
SphI GCATGC 2 cut(s) 611, 1040
Sse9I AATT 7 cut(s) 407, 849, 938, 1044, 1213, 1715, 1941
SseBI AGGCCT 2 cut(s) 975, 2118
SsiI CCGC 6 cut(s) 267, 322, 529, 815, 1589, 1870
SspDI GGCGCC 1 cut(s) 260
SspMI CTAG 2 cut(s) 2223, 2405
SstI GAGCTC 1 cut(s) 2061
StuI AGGCCT 2 cut(s) 975, 2118
StyD4I CCNGG 8 cut(s) 162, 220, 639, 1126, 1344, 1648, 1720, 1888
StyI CCWWGG 3 cut(s) 426, 864, 1534
TaaI ACNGT 7 cut(s) 130, 475, 900, 1181, 1832, 1972, 2311
TaiI ACGT 3 cut(s) 15, 606, 1797
TaqI TCGA 4 cut(s) 33, 50, 552, 714
TasI AATT 7 cut(s) 407, 849, 938, 1044, 1213, 1715, 1941
TatI WGTACW 3 cut(s) 91, 883, 1276
TfiI GAWTC 3 cut(s) 30, 563, 1498
Tru1I TTAA 8 cut(s) 57, 69, 345, 786, 1043, 1266, 2040, 2422
Tru9I TTAA 8 cut(s) 57, 69, 345, 786, 1043, 1266, 2040, 2422
TscAI CASTG 9 cut(s) 402, 1105, 1420, 1645, 1906, 2031, 2037, 2146, 2297
TseFI GTSAC 4 cut(s) 130, 757, 1949, 2032
TseI GCWGC 4 cut(s) 1003, 1073, 1586, 1604
Tsp45I GTSAC 4 cut(s) 130, 757, 1949, 2032
TspDTI ATGAA 8 cut(s) 244, 316, 400, 549, 948, 1008, 1220, 1559
TspGWI ACGGA 5 cut(s) 189, 494, 1401, 1697, 2342
TspRI CASTG 9 cut(s) 402, 1105, 1420, 1645, 1906, 2031, 2037, 2146, 2297
VneI GTGCAC 1 cut(s) 730
VpaK11BI GGWCC 2 cut(s) 225, 1684
XapI RAATTY 1 cut(s) 938
XceI RCATGY 7 cut(s) 611, 670, 986, 1040, 1405, 2131, 2159
XcmI CCANNNNNNNNNTGG 1 cut(s) 2146
XhoI CTCGAG 1 cut(s) 551
XmnI GAANNNNTTC 1 cut(s) 1208
XspI CTAG 2 cut(s) 2223, 2405
ZrmI AGTACT 1 cut(s) 93
Zsp2I ATGCAT 2 cut(s) 1394, 1885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.