Rh7AG122800

Galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000881
Physical Location & Seq
Reverse (-)
9622405 .. 9623805
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG122800.1

Sequence Viewer

Length: 777 bp
ATGACTATGACCTCTACCGAGCCAAGCAGCAGAGATGGTTGCTTCATGGTCCGTGACAATGCCCTTCTGACACGTGTCCCGGCCAACATCACCGTCACTCCGGCCACTGATAAATCAGCTTTCATCGGAGCCACCTCTGAAGACCGTAGCTCTCGCCATGTTTTCCCTCTCGGACTTCTTGAGGGATACAAATTTATGTGCCTGTTCAGATTCAAAATCTGGTGGATGATACCGAGTTTTGGGGATTCTGGATGTGATGTTCCAGCAGAGACTCAAATGCTTCTCTTGGAAGCAACGCAAACGTCGAGTGGTAGTAGTATTCCCGACAAAAATGGGTTCTCATCTGAGTCTGAGGAGCCAACAAGTGCTGAGAACAAAAGCACTTTCTACATTCTGCTATTGCCAGTCTTGGATGGAGCATTCAGGGCTACTTTGCAAGGCAACACTGTTAATGAGCTTGAGTTTTGCGTTGAAAGCGGGGACCGTGAAGTTCAAACCTCACGAGTAACTGAAGCAGTATTTGTGAATTCAGGAGATGACCCATATAAGCTGATTAGGGACTCTATTAAGATTCTTGCCAATCACAAAGGTACATTCAATCATTTAGAAGACAAGAAGATACCAGCACATGTAGACTGGTTTGGATGGAACACATGGGATGCATTTTATAATGACGTTGATCCGGAAGGAATCGAGCTTGGACTCAAAAGTCTTTCCGAAGGAGGGTGCCCCCCGAAATGTTTGTACATCGATGAAGGATGGCAGACTAAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

258

Amino Acids

28.65

Weight (kDa)

4.7

Isoelectric Point (pI)

44.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 12 - 256 6.8e-79 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AasI GACNNNNNNGTC 1 cut(s) 708
AccB1I GGYRCC 1 cut(s) 726
AccI GTMKAC 1 cut(s) 633
AccIII TCCGGA 1 cut(s) 682
AciI CCGC 1 cut(s) 477
AclWI GGATC 1 cut(s) 674
AcoI YGGCCR 2 cut(s) 81, 102
AcsI RAATTY 2 cut(s) 191, 526
AcuI CTGAAG 2 cut(s) 159, 531
AcvI CACGTG 1 cut(s) 74
AfaI GTAC 2 cut(s) 592, 746
AfiI CCNNNNNNNGG 2 cut(s) 239, 723
AflIII ACRYGT 3 cut(s) 71, 73, 628
AgsI TTSAA 4 cut(s) 214, 473, 494, 598
AloI GAACNNNNNNTCC 2 cut(s) 243, 275
AluBI AGCT 5 cut(s) 119, 150, 457, 550, 697
AluI AGCT 5 cut(s) 119, 150, 457, 550, 697
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 1 cut(s) 674
Aor13HI TCCGGA 1 cut(s) 682
AoxI GGCC 2 cut(s) 81, 102
ApeKI GCWGC 1 cut(s) 27
ApoI RAATTY 2 cut(s) 191, 526
AspS9I GGNCC 2 cut(s) 49, 481
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 82
AvaII GGWCC 2 cut(s) 49, 481
BaeGI GKGCMC 1 cut(s) 731
BanI GGYRCC 1 cut(s) 726
BauI CACGAG 1 cut(s) 501
BbrPI CACGTG 1 cut(s) 74
BbsI GAAGAC 2 cut(s) 147, 615
BbvI GCAGC 1 cut(s) 39
BccI CCATC 4 cut(s) 29, 407, 639, 753
BciVI GTATCC 1 cut(s) 179
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 1 cut(s) 263
BfuI GTATCC 1 cut(s) 179
BisI GCNGC 1 cut(s) 28
BlsI GCNGC 1 cut(s) 29
Bme1390I CCNGG 1 cut(s) 80
Bme18I GGWCC 2 cut(s) 49, 481
BmgT120I GGNCC 2 cut(s) 49, 481
BmiI GGNNCC 4 cut(s) 130, 357, 482, 728
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 1 cut(s) 649
BoxI GACNNNNGTC 1 cut(s) 74
BpiI GAAGAC 2 cut(s) 147, 615
BpuEI CTTGAG 2 cut(s) 200, 479
BpuMI CCSGG 1 cut(s) 80
Bsa29I ATCGAT 1 cut(s) 750
BsaAI YACGTR 1 cut(s) 74
BsaWI WCCGGW 1 cut(s) 682
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 723
Bse1I ACTGG 2 cut(s) 404, 641
BseAI TCCGGA 1 cut(s) 682
BseCI ATCGAT 1 cut(s) 750
BseGI GGATG 6 cut(s) 231, 257, 418, 650, 664, 764
BseLI CCNNNNNNNGG 2 cut(s) 239, 723
BseMII CTCAG 3 cut(s) 336, 342, 360
BseNI ACTGG 2 cut(s) 404, 641
BseRI GAGGAG 1 cut(s) 368
BseSI GKGCMC 1 cut(s) 731
BseXI GCAGC 1 cut(s) 39
BshFI GGCC 2 cut(s) 83, 104
BshNI GGYRCC 1 cut(s) 726
BshVI ATCGAT 1 cut(s) 750
BsiSI CCGG 3 cut(s) 80, 101, 683
BslFI GGGAC 3 cut(s) 62, 494, 572
BslI CCNNNNNNNGG 2 cut(s) 239, 723
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 3 cut(s) 62, 494, 572
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 2 cut(s) 83, 104
Bsp1286I GDGCHC 1 cut(s) 731
Bsp13I TCCGGA 1 cut(s) 682
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 1 cut(s) 679
BspACI CCGC 1 cut(s) 477
BspANI GGCC 2 cut(s) 83, 104
BspCNI CTCAG 3 cut(s) 337, 343, 361
BspDI ATCGAT 1 cut(s) 750
BspEI TCCGGA 1 cut(s) 682
BspLI GGNNCC 4 cut(s) 130, 357, 482, 728
BspPI GGATC 1 cut(s) 674
BspT107I GGYRCC 1 cut(s) 726
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 2 cut(s) 404, 641
BssMI GATC 1 cut(s) 679
BssSI CACGAG 1 cut(s) 501
Bst2BI CACGAG 1 cut(s) 501
Bst4CI ACNGT 4 cut(s) 94, 146, 448, 485
BstAUI TGTACA 1 cut(s) 744
BstBAI YACGTR 1 cut(s) 74
BstDEI CTNAG 3 cut(s) 345, 351, 369
BstF5I GGATG 6 cut(s) 231, 257, 418, 650, 664, 764
BstKTI GATC 1 cut(s) 682
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 1 cut(s) 679
BstMWI GCNNNNNNNGC 2 cut(s) 425, 474
BstNSI RCATGY 1 cut(s) 632
BstPAI GACNNNNGTC 1 cut(s) 74
BstSCI CCNGG 1 cut(s) 78
BstSLI GKGCMC 1 cut(s) 731
BstV1I GCAGC 1 cut(s) 39
BstV2I GAAGAC 2 cut(s) 147, 615
Bsu15I ATCGAT 1 cut(s) 750
BsuI GTATCC 1 cut(s) 179
BsuRI GGCC 2 cut(s) 83, 104
BsuTUI ATCGAT 1 cut(s) 750
BtsCI GGATG 6 cut(s) 231, 257, 418, 650, 664, 764
BtsIMutI CAGTG 2 cut(s) 105, 444
Cfr13I GGNCC 2 cut(s) 49, 481
ClaI ATCGAT 1 cut(s) 750
Csp6I GTAC 2 cut(s) 591, 745
CviAII CATG 4 cut(s) 46, 158, 629, 654
CviQI GTAC 2 cut(s) 591, 745
DdeI CTNAG 3 cut(s) 345, 351, 369
DpnI GATC 1 cut(s) 681
DpnII GATC 1 cut(s) 679
DrdI GACNNNNNNGTC 1 cut(s) 708
DseDI GACNNNNNNGTC 1 cut(s) 708
EaeI YGGCCR 2 cut(s) 81, 102
Eco47I GGWCC 2 cut(s) 49, 481
Eco57I CTGAAG 2 cut(s) 159, 531
Eco72I CACGTG 1 cut(s) 74
EcoRI GAATTC 1 cut(s) 526
EcoT22I ATGCAT 1 cut(s) 664
FaeI CATG 4 cut(s) 49, 161, 632, 657
FaqI GGGAC 3 cut(s) 62, 494, 572
FatI CATG 4 cut(s) 45, 157, 628, 653
FauI CCCGC 1 cut(s) 470
FblI GTMKAC 1 cut(s) 633
Fnu4HI GCNGC 1 cut(s) 28
FokI GGATG 6 cut(s) 238, 264, 425, 657, 671, 771
Fsp4HI GCNGC 1 cut(s) 28
GluI GCNGC 1 cut(s) 28
HaeIII GGCC 2 cut(s) 83, 104
HapII CCGG 3 cut(s) 80, 101, 683
Hin1II CATG 4 cut(s) 49, 161, 632, 657
HinfI GANTC 8 cut(s) 210, 245, 271, 347, 560, 571, 690, 702
HpaII CCGG 3 cut(s) 80, 101, 683
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 1 cut(s) 634
Hpy188I TCNGA 8 cut(s) 69, 128, 139, 173, 209, 346, 352, 718
Hpy188III TCNNGA 6 cut(s) 179, 249, 323, 501, 531, 683
Hpy8I GTNNAC 1 cut(s) 634
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 4 cut(s) 74, 680, 713, 749
HpyCH4III ACNGT 4 cut(s) 94, 146, 448, 485
HpyCH4IV ACGT 3 cut(s) 73, 302, 675
HpyCH4V TGCA 2 cut(s) 436, 662
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 474
HpyF3I CTNAG 3 cut(s) 345, 351, 369
HpySE526I ACGT 3 cut(s) 73, 302, 675
Hsp92II CATG 4 cut(s) 49, 161, 632, 657
Kpn2I TCCGGA 1 cut(s) 682
Kzo9I GATC 1 cut(s) 679
LmnI GCTCC 3 cut(s) 128, 355, 416
Lsp1109I GCAGC 1 cut(s) 39
LweI GCATC 1 cut(s) 649
MaeII ACGT 3 cut(s) 73, 302, 675
MaeIII GTNAC 3 cut(s) 53, 94, 505
MalI GATC 1 cut(s) 681
MboI GATC 1 cut(s) 679
MboII GAAGA 3 cut(s) 152, 620, 628
MhlI GDGCHC 1 cut(s) 731
MluCI AATT 2 cut(s) 191, 526
MlyI GAGTC 4 cut(s) 265, 356, 554, 696
MnlI CCTC 7 cut(s) 22, 145, 175, 177, 346, 508, 716
Mph1103I ATGCAT 1 cut(s) 664
MroI TCCGGA 1 cut(s) 682
MseI TTAA 2 cut(s) 450, 567
MspI CCGG 3 cut(s) 80, 101, 683
MspR9I CCNGG 1 cut(s) 80
Mva1269I GAATGC 1 cut(s) 419
MwoI GCNNNNNNNGC 2 cut(s) 425, 474
NciI CCSGG 1 cut(s) 80
NdeII GATC 1 cut(s) 679
NlaIII CATG 4 cut(s) 49, 161, 632, 657
NlaIV GGNNCC 4 cut(s) 130, 357, 482, 728
NmuCI GTSAC 2 cut(s) 53, 94
NsiI ATGCAT 1 cut(s) 664
NspI RCATGY 1 cut(s) 632
PciI ACATGT 1 cut(s) 628
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PctI GAATGC 1 cut(s) 419
PfeI GAWTC 4 cut(s) 210, 245, 571, 690
PkrI GCNGC 1 cut(s) 29
PleI GAGTC 4 cut(s) 265, 355, 554, 696
PmaCI CACGTG 1 cut(s) 74
PmlI CACGTG 1 cut(s) 74
PpsI GAGTC 4 cut(s) 265, 355, 554, 696
Ppu21I YACGTR 1 cut(s) 74
PscI ACATGT 1 cut(s) 628
PshAI GACNNNNGTC 1 cut(s) 74
PsiI TTATAA 1 cut(s) 669
PspCI CACGTG 1 cut(s) 74
PspN4I GGNNCC 4 cut(s) 130, 357, 482, 728
PspPI GGNCC 2 cut(s) 49, 481
RsaI GTAC 2 cut(s) 592, 746
RsaNI GTAC 2 cut(s) 591, 745
SaqAI TTAA 2 cut(s) 450, 567
SatI GCNGC 1 cut(s) 28
Sau3AI GATC 1 cut(s) 679
Sau96I GGNCC 2 cut(s) 49, 481
SchI GAGTC 4 cut(s) 265, 356, 554, 696
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 1 cut(s) 731
SfaNI GCATC 1 cut(s) 649
SinI GGWCC 2 cut(s) 49, 481
SmlI CTYRAG 2 cut(s) 179, 458
SmoI CTYRAG 2 cut(s) 179, 458
Sse9I AATT 2 cut(s) 191, 526
SsiI CCGC 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 78
TaaI ACNGT 4 cut(s) 94, 146, 448, 485
TaiI ACGT 3 cut(s) 76, 305, 678
TaqI TCGA 3 cut(s) 305, 693, 750
TasI AATT 2 cut(s) 191, 526
TatI WGTACW 1 cut(s) 744
TfiI GAWTC 4 cut(s) 210, 245, 571, 690
Tru1I TTAA 2 cut(s) 450, 567
Tru9I TTAA 2 cut(s) 450, 567
TscAI CASTG 2 cut(s) 112, 451
TseFI GTSAC 2 cut(s) 53, 94
TseI GCWGC 1 cut(s) 27
Tsp45I GTSAC 2 cut(s) 53, 94
TspDTI ATGAA 3 cut(s) 34, 112, 768
TspGWI ACGGA 1 cut(s) 41
TspRI CASTG 2 cut(s) 112, 451
VpaK11BI GGWCC 2 cut(s) 49, 481
XapI RAATTY 2 cut(s) 191, 526
XceI RCATGY 1 cut(s) 632
XmiI GTMKAC 1 cut(s) 633
Zsp2I ATGCAT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.