RchiOBHm_Chr7g0192011

Galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000881
Physical Location & Seq
Reverse (-)
10644539 .. 10646311
1773 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17158

Sequence Viewer

Length: 1170 bp
ATGATTTATAGATTTTACGATGATCTACATAGCTACCTTGCAAGTTGCAATATAGACGGAGTGAAGGTGGATATACACAATGAAGTGGAACTCCTTGCTTCTGGTTATGGAGGCCGCGTTGCATTGATGAGGCATTTCCAAGAAGCCCTCGAGGAATCTGTCATGAGAAACTTTGGCAGCGATAACCTTATTTGTTCCATGAGTCTAAGCAATGACTACATTTACAGCTCAAAGAAGAGTGCAGCTTCAAGAGTGTCAGAGGACTTCATGCCACTAGAGAAAACATTTCAGACCCTACATGTTGCTGCAGTAGCTTTTAACAGTCTTCTTATGGGAGAGATTGTTGTACCAGATTGGGACATGCTCTTTAGCGACCACTACACCCGGGAGTTCCATGCTGCAGCCAGAGCATTGGGTGGATGTCCAGTATATGTAAGTGACAAGCCTGGCAGCCATAATTTCAATGTCCTCAAAAAGCTTGTACTGCCTGATGGCTCTATTCTAAGAGCTAGATTCGCTGGCCGGCCTACTAGGGACTGCTTATTCAGTGACCCTGTGGTCGATGGAAAAAGTCTGCTGAAGATTTGGAATCTAAACAAGGTATCAGGAGTTATTGGTGTTTTCAATTGCCAAAGAGCAGGAAAGTGGCCACCAATTGCAGGTGCTCAATATGTGCCTTCCTCTGAATCTGCACCGCCTCTTATAGGCCTCGTCAGCCCCATAGATATTAATATGCTTGAAGATGTTGCAAATGAAAGTTGGAGAGGAGAATGTGCAGTATATGCATACCATTCAGGTACTCTTTCTGTAATGCCAAAGAAAGACCACTTTGAAGTATCCTTGGATGTGCTAGAATGTGAAGTTTTCACCATATCTCCTATTATGGTTTTTGGTGACAACCTTCTATTTGCCCCAATGGGGTTGCTTGATATGTACAATTCAGGAGGAGCACTTGAATCTCTAGATGTCAGCAACAATGATCTGTTCGACTGTGTTGTGAAGGTTCGAGTCAGAGGATGTGGTCGATTTGGAGCCTACTCAAACAAAAAACCAAAGTCTTGTCTGGTGAACAAGAAAGAGGAGTTTATAGTCTACAATGCTAATAATGGTTTGTTGGTACTAAAACTTCAAGGCGACTGCAAAGTAAAAGAGATTGAGTTCATGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

389

Amino Acids

43.16

Weight (kDa)

5.64

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 2 - 375 1.3e-132 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 650
AasI GACNNNNNNGTC 1 cut(s) 557
AbsI CCTCGAGG 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 650
AccI GTMKAC 1 cut(s) 1092
AccII CGCG 1 cut(s) 117
AciI CCGC 2 cut(s) 115, 695
AcoI YGGCCR 2 cut(s) 520, 647
AcuI CTGAAG 1 cut(s) 599
AfaI GTAC 6 cut(s) 348, 483, 799, 935, 1119, 1166
AfiI CCNNNNNNNGG 3 cut(s) 659, 704, 918
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 7 cut(s) 249, 463, 625, 740, 833, 956, 1130
AjnI CCWGG 1 cut(s) 445
AluBI AGCT 6 cut(s) 33, 228, 245, 314, 478, 509
AluI AGCT 6 cut(s) 33, 228, 245, 314, 478, 509
Alw21I GWGCWC 2 cut(s) 667, 952
Ama87I CYCGRG 2 cut(s) 149, 384
AoxI GGCC 5 cut(s) 112, 520, 524, 647, 706
ApeKI GCWGC 6 cut(s) 177, 242, 305, 398, 401, 450
AseI ATTAAT 1 cut(s) 729
AsuC2I CCSGG 2 cut(s) 385, 386
AsuHPI GGTGA 3 cut(s) 859, 905, 1078
AvaI CYCGRG 2 cut(s) 149, 384
BalI TGGCCA 1 cut(s) 649
BarI GAAGNNNNNNTAC 2 cut(s) 1110, 1142
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 2 cut(s) 667, 952
BbvI GCAGC 6 cut(s) 189, 254, 292, 385, 413, 462
BccI CCATC 2 cut(s) 485, 557
BciT130I CCWGG 1 cut(s) 447
BciVI GTATCC 1 cut(s) 847
BcnI CCSGG 2 cut(s) 385, 386
BfaI CTAG 5 cut(s) 275, 510, 531, 851, 962
BfmI CTRYAG 2 cut(s) 306, 399
BfuAI ACCTGC 1 cut(s) 650
BfuI GTATCC 1 cut(s) 847
BisI GCNGC 7 cut(s) 115, 178, 243, 306, 399, 402, 451
BlsI GCNGC 7 cut(s) 116, 179, 244, 307, 400, 403, 452
Bme1390I CCNGG 3 cut(s) 385, 386, 447
BmeT110I CYCGRG 2 cut(s) 149, 384
BmiI GGNNCC 1 cut(s) 1033
BmrFI CCNGG 3 cut(s) 385, 386, 447
BpiI GAAGAC 1 cut(s) 317
BpuMI CCSGG 2 cut(s) 385, 386
BsaJI CCNNGG 2 cut(s) 384, 840
BsaXI ACNNNNNCTCC 8 cut(s) 51, 81, 102, 132, 327, 357, 859, 889
Bsc4I CCNNNNNNNGG 3 cut(s) 659, 704, 918
Bse118I RCCGGY 1 cut(s) 522
Bse1I ACTGG 1 cut(s) 425
Bse3DI GCAATG 1 cut(s) 217
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 384, 840
BseGI GGATG 3 cut(s) 425, 850, 1022
BseLI CCNNNNNNNGG 3 cut(s) 659, 704, 918
BseMI GCAATG 1 cut(s) 217
BseNI ACTGG 1 cut(s) 425
BseRI GAGGAG 3 cut(s) 780, 960, 1094
BseXI GCAGC 6 cut(s) 189, 254, 292, 385, 413, 462
BsgI GTGCAG 3 cut(s) 261, 675, 795
Bsh1236I CGCG 1 cut(s) 117
BshFI GGCC 5 cut(s) 114, 522, 526, 649, 708
BsiHKAI GWGCWC 2 cut(s) 667, 952
BsiHKCI CYCGRG 2 cut(s) 149, 384
BsiSI CCGG 2 cut(s) 385, 523
BslFI GGGAC 2 cut(s) 371, 548
BslI CCNNNNNNNGG 3 cut(s) 659, 704, 918
BsmFI GGGAC 2 cut(s) 371, 548
BsnI GGCC 5 cut(s) 114, 522, 526, 649, 708
BsoBI CYCGRG 2 cut(s) 149, 384
Bsp1286I GDGCHC 2 cut(s) 667, 952
Bsp1407I TGTACA 1 cut(s) 933
Bsp143I GATC 2 cut(s) 22, 979
BspACI CCGC 2 cut(s) 115, 695
BspANI GGCC 5 cut(s) 114, 522, 526, 649, 708
BspFNI CGCG 1 cut(s) 117
BspHI TCATGA 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 1033
BspMAI CTGCAG 2 cut(s) 310, 403
BspMI ACCTGC 1 cut(s) 650
BsrDI GCAATG 1 cut(s) 217
BsrFI RCCGGY 1 cut(s) 522
BsrGI TGTACA 1 cut(s) 933
BsrI ACTGG 1 cut(s) 425
BssAI RCCGGY 1 cut(s) 522
BssECI CCNNGG 2 cut(s) 384, 840
BssMI GATC 2 cut(s) 22, 979
BssT1I CCWWGG 1 cut(s) 840
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 2 cut(s) 323, 992
Bst6I CTCTTC 1 cut(s) 230
BstAPI GCANNNNNTGC 1 cut(s) 782
BstAUI TGTACA 1 cut(s) 933
BstC8I GCNNGC 2 cut(s) 520, 524
BstDEI CTNAG 2 cut(s) 206, 503
BstENI CCTNNNNNAGG 1 cut(s) 702
BstF5I GGATG 3 cut(s) 425, 850, 1022
BstFNI CGCG 1 cut(s) 117
BstKTI GATC 2 cut(s) 25, 982
BstMBI GATC 2 cut(s) 22, 979
BstMWI GCNNNNNNNGC 6 cut(s) 311, 407, 484, 515, 714, 782
BstNI CCWGG 1 cut(s) 447
BstNSI RCATGY 2 cut(s) 302, 364
BstSCI CCNGG 3 cut(s) 383, 384, 445
BstSFI CTRYAG 2 cut(s) 306, 399
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 6 cut(s) 189, 254, 292, 385, 413, 462
BstV2I GAAGAC 1 cut(s) 317
BstXI CCANNNNNNTGG 1 cut(s) 412
BsuI GTATCC 1 cut(s) 847
BsuRI GGCC 5 cut(s) 114, 522, 526, 649, 708
BtsCI GGATG 3 cut(s) 425, 850, 1022
BtsIMutI CAGTG 1 cut(s) 553
BveI ACCTGC 1 cut(s) 650
Cac8I GCNNGC 2 cut(s) 520, 524
CciI TCATGA 1 cut(s) 162
Cfr10I RCCGGY 1 cut(s) 522
Cfr9I CCCGGG 1 cut(s) 384
Csp6I GTAC 6 cut(s) 347, 482, 798, 934, 1118, 1165
CviAII CATG 7 cut(s) 163, 199, 268, 299, 361, 395, 1162
CviQI GTAC 6 cut(s) 347, 482, 798, 934, 1118, 1165
DdeI CTNAG 2 cut(s) 206, 503
DpnI GATC 2 cut(s) 24, 981
DpnII GATC 2 cut(s) 22, 979
DrdI GACNNNNNNGTC 1 cut(s) 557
DseDI GACNNNNNNGTC 1 cut(s) 557
EaeI YGGCCR 2 cut(s) 520, 647
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 840
Eco147I AGGCCT 1 cut(s) 708
Eco57I CTGAAG 1 cut(s) 599
Eco88I CYCGRG 2 cut(s) 149, 384
EcoNI CCTNNNNNAGG 1 cut(s) 702
EcoRII CCWGG 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 840
EcoT22I ATGCAT 1 cut(s) 787
ErhI CCWWGG 1 cut(s) 840
FaeI CATG 7 cut(s) 166, 202, 271, 302, 364, 398, 1165
FaqI GGGAC 2 cut(s) 371, 548
FatI CATG 7 cut(s) 162, 198, 267, 298, 360, 394, 1161
FblI GTMKAC 1 cut(s) 1092
Fnu4HI GCNGC 7 cut(s) 115, 178, 243, 306, 399, 402, 451
FokI GGATG 3 cut(s) 432, 857, 1029
FseI GGCCGGCC 1 cut(s) 526
Fsp4HI GCNGC 7 cut(s) 115, 178, 243, 306, 399, 402, 451
FspBI CTAG 5 cut(s) 275, 510, 531, 851, 962
GluI GCNGC 7 cut(s) 115, 178, 243, 306, 399, 402, 451
HaeIII GGCC 5 cut(s) 114, 522, 526, 649, 708
HapII CCGG 2 cut(s) 385, 523
Hin1II CATG 7 cut(s) 166, 202, 271, 302, 364, 398, 1165
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 7 cut(s) 155, 202, 513, 589, 686, 956, 1008
HpaII CCGG 2 cut(s) 385, 523
HphI GGTGA 3 cut(s) 859, 905, 1078
Hpy166II GTNNAC 2 cut(s) 1069, 1093
Hpy188I TCNGA 4 cut(s) 259, 291, 685, 1013
Hpy188III TCNNGA 5 cut(s) 163, 249, 606, 942, 962
Hpy8I GTNNAC 2 cut(s) 1069, 1093
HpyAV CCTTC 4 cut(s) 58, 687, 911, 994
HpyCH4III ACNGT 2 cut(s) 323, 992
HpyF10VI GCNNNNNNNGC 6 cut(s) 311, 407, 484, 515, 714, 782
HpyF3I CTNAG 2 cut(s) 206, 503
Hsp92II CATG 7 cut(s) 166, 202, 271, 302, 364, 398, 1165
KroI GCCGGC 1 cut(s) 522
KroNI GCCGGC 1 cut(s) 524
Kzo9I GATC 2 cut(s) 22, 979
LmnI GCTCC 2 cut(s) 947, 1031
Lsp1109I GCAGC 6 cut(s) 189, 254, 292, 385, 413, 462
MaeI CTAG 5 cut(s) 275, 510, 531, 851, 962
MaeIII GTNAC 3 cut(s) 437, 548, 893
MalI GATC 2 cut(s) 24, 981
MboI GATC 2 cut(s) 22, 979
MboII GAAGA 4 cut(s) 247, 317, 592, 752
MfeI CAATTG 2 cut(s) 625, 654
MhlI GDGCHC 2 cut(s) 667, 952
MlsI TGGCCA 1 cut(s) 649
MluCI AATT 4 cut(s) 457, 625, 654, 937
MluNI TGGCCA 1 cut(s) 649
MlyI GAGTC 2 cut(s) 211, 1017
MmeI TCCRAC 1 cut(s) 740
Mox20I TGGCCA 1 cut(s) 649
Mph1103I ATGCAT 1 cut(s) 787
MroNI GCCGGC 1 cut(s) 522
MscI TGGCCA 1 cut(s) 649
MseI TTAA 2 cut(s) 318, 729
Msp20I TGGCCA 1 cut(s) 649
MspI CCGG 2 cut(s) 385, 523
MspR9I CCNGG 3 cut(s) 385, 386, 447
MunI CAATTG 2 cut(s) 625, 654
MvaI CCWGG 1 cut(s) 447
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 6 cut(s) 311, 407, 484, 515, 714, 782
NaeI GCCGGC 1 cut(s) 524
NciI CCSGG 2 cut(s) 385, 386
NdeII GATC 2 cut(s) 22, 979
NgoMIV GCCGGC 1 cut(s) 522
NlaIII CATG 7 cut(s) 166, 202, 271, 302, 364, 398, 1165
NlaIV GGNNCC 1 cut(s) 1033
NmuCI GTSAC 3 cut(s) 437, 548, 893
NsiI ATGCAT 1 cut(s) 787
NspI RCATGY 2 cut(s) 302, 364
PaeR7I CTCGAG 1 cut(s) 149
PagI TCATGA 1 cut(s) 162
PaqCI CACCTGC 1 cut(s) 650
PceI AGGCCT 1 cut(s) 708
PciI ACATGT 1 cut(s) 298
PdiI GCCGGC 1 cut(s) 524
PfeI GAWTC 5 cut(s) 155, 513, 589, 686, 956
PkrI GCNGC 7 cut(s) 116, 179, 244, 307, 400, 403, 452
PleI GAGTC 2 cut(s) 210, 1016
PpsI GAGTC 2 cut(s) 210, 1016
PscI ACATGT 1 cut(s) 298
PshBI ATTAAT 1 cut(s) 729
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 1 cut(s) 1033
PspXI VCTCGAGB 1 cut(s) 149
PstI CTGCAG 2 cut(s) 310, 403
RigI GGCCGGCC 1 cut(s) 526
RsaI GTAC 6 cut(s) 348, 483, 799, 935, 1119, 1166
RsaNI GTAC 6 cut(s) 347, 482, 798, 934, 1118, 1165
SaqAI TTAA 2 cut(s) 318, 729
SatI GCNGC 7 cut(s) 115, 178, 243, 306, 399, 402, 451
Sau3AI GATC 2 cut(s) 22, 979
SchI GAGTC 2 cut(s) 211, 1017
ScrFI CCNGG 3 cut(s) 385, 386, 447
SduI GDGCHC 2 cut(s) 667, 952
SfcI CTRYAG 2 cut(s) 306, 399
Sfr274I CTCGAG 1 cut(s) 149
SlaI CTCGAG 1 cut(s) 149
SmaI CCCGGG 1 cut(s) 386
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 4 cut(s) 457, 625, 654, 937
SseBI AGGCCT 1 cut(s) 708
SsiI CCGC 2 cut(s) 115, 695
SspMI CTAG 5 cut(s) 275, 510, 531, 851, 962
StuI AGGCCT 1 cut(s) 708
StyD4I CCNGG 3 cut(s) 383, 384, 445
StyI CCWWGG 1 cut(s) 840
TaaI ACNGT 2 cut(s) 323, 992
TaqI TCGA 5 cut(s) 150, 561, 987, 1006, 1024
TasI AATT 4 cut(s) 457, 625, 654, 937
TatI WGTACW 3 cut(s) 481, 933, 1164
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 5 cut(s) 155, 513, 589, 686, 956
Tru1I TTAA 2 cut(s) 318, 729
Tru9I TTAA 2 cut(s) 318, 729
TscAI CASTG 1 cut(s) 553
TseFI GTSAC 3 cut(s) 437, 548, 893
TseI GCWGC 6 cut(s) 177, 242, 305, 398, 401, 450
Tsp45I GTSAC 3 cut(s) 437, 548, 893
TspDTI ATGAA 4 cut(s) 96, 256, 768, 1150
TspGWI ACGGA 1 cut(s) 72
TspMI CCCGGG 1 cut(s) 384
TspRI CASTG 1 cut(s) 553
VspI ATTAAT 1 cut(s) 729
XagI CCTNNNNNAGG 1 cut(s) 702
XbaI TCTAGA 1 cut(s) 961
XceI RCATGY 2 cut(s) 302, 364
XhoI CTCGAG 1 cut(s) 149
XmaI CCCGGG 1 cut(s) 384
XmiI GTMKAC 1 cut(s) 1092
XspI CTAG 5 cut(s) 275, 510, 531, 851, 962
Zsp2I ATGCAT 1 cut(s) 787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.