Rh5DG001600

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000881
Physical Location & Seq
Reverse (-)
113441 .. 118880
5440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG001600.1

Sequence Viewer

Length: 2424 bp
ATGCTTCACAGCACCGTCGTTTCCCCCAAGGCCCTTCAACTCAATCTCGGCTTCTCTTCATTTCTAGCCACAAACCAGAACCAGAGCAGGAGGAGGAGGAGGATCATCTTTTGCTCACAAACCAGAGATGGTTCCGTGCGTGGTGGAGTTAAGACTAGCACTACTAGTAGTAATGGATGGAGACAATCTATGTTTGTCGGTATGAAACCTGCCCTTGAAGATTCCATTCTGAGTGTCAGTGGTAAACATGTGTTGACAGACGTGCCTCCCCATGTGGTCTTCACACCAATTCCCAACTCATCGGCAGCATTCCTTGGCGCCACTTCTCAAAATGCTACTTCACAATCACGCCATGTTTTTAAGCTCGGAGTCTTACGTGATGTCAGATTATTAAGTCTTTTTAGATTTAAACTTTGGTGGATGATACCACGAGTGGGCAGTACAGGAAGTGACATCCCTGTGGAAACTCAGCTGTTGCTCCTAGAAGCAAAGGAGGAGGAGGGAAAGGAAGATAGTACATCTTATATCTTATTCTTGCCAGTTCTGGATGGTGAATTTAGAAGCAGCTTGCAGGGAAATACATCCAATGAACTTGAGTTTTGTGTCGAAAGTGGTGACCCCGCTATAGTTGCCTCAGAATCCCTAAAAGCAGTTTTTGTGAATTGTGGGAACCATCCTTTCGATCTGGTGAACGAATCTATGAAGACTTTGGCGAAGCATTATGGAAGCTTTGCTCTTAGAGAAACGAAACAGATGCCTGGAATGCTGGATTGTTTTGGCTGGTGTACTTGGGATGCCTTTTATCAAGGAGTTAACCCTAAAGGAATTAGAGACGGCCTCAGAAGTTTATCAGAAGGAGGTACTCCAGCTAAGTTTTTGATAATCGATGATGGGTGGCAAGATACATCAAATGAGTTCCAAAAAGAAGGGGAGCCATTTGTTGAAGGGACACAGTTTGGAGGCAGATTAAACAGCATTGAAGAAAATAACAAGTTTCGCAGCACAACCAATATGGTTGATAGTGACAAACCAAGTGGTCTCAAGGATTTTGTTTCTGAGATCAAGAATTCCTTTGGCCTTAGGTATGTGTATGTATGGCATGCCCTTTTGGGATATTGGGGAGGGCTTGTTCCAAATGCTCCAGGAACTAAAAAGTATAACCCTAAGTTCAGACACCCAGTACAGTCACCTGGAAATTTGTCAAATATGAGGGATGGAGCTATGGATAGTATGGAGAAATATGGTGTTGGTGTAATAGATCCTGCAAAGGCATATCAATTTTATGATGATCTACACGGATATCTTGTTTCACAGGATGTAGATGGTGTTAAGGTTGATGTTCAGAACATACTGGAGACAGTATCAGCAGGTTTAGGAGGGCGAGTCTCTCTAACTAGACGATTCCAACAGGCACTTGAGAAGTCCATAGCCACTCATTTCCAAGATAACAGCATAATCTGCTGCATGGGCCAAAGCACAGACTCCATTTACCATTCAAAAAGAAGTGCTATTACTCGTGCATCTGATGATTACTACCCAAAAAACCCAACAACACAGACACTGCACATTGCTGCAGTGGCTTTCAACAGCATATTCCTTGGTGAAGTTTTTGTCCCAGATTGGGACATGTTCTATAGCCGGCATGAGGCAGCTGAGTTCCATGCTGCTGCTAGAGCTGTGGGGGGTTGCGGAGTCTATGTAAGTGACAAACCTGGCCAGCATGATTTCGAGATACTTAAAAGGCTTGTTCTTCCTGATGGAACAGTTCTCAGAGCTAGATATCCTGGGAGGCCATCACGGGATTGTTTATTTGTTGATCCAGTTATGGACGGGAAGAGTCTTCTGAAAATATGGAATTTGAACAAATGCAACGGAGTTATAGGCATTTTCAATTGCCAAGGAGCAGGAAGCTGGCCTTGTCTGGGACATATCGTCCAGGTAAAAGCTAGTGAGGAGCTATCTGGGAAGGTCTCTCCTGCCGATATCGAGTATTTTGAAGAGGTATCTGGAAAGCTTTGGACTGGAGATTGTGCAGTCTATTCCTTCAAACAAGGGTATCTGTCTCGTTTACCGAAGGACAAGTCATTTGATGTCACATTACAAATTCTCCAATGCGATGTGTTTACTGTATCCCCTATTAAGGTTTACAAGCAAAATATTCAATTTGCACCTATCGGATTATTGAACATGTACAATTCCGGTGGAGCTGTTGACAGTATTGACTTGTTCAGTGATGCCTCTAGTTGTGTTATACACATCAAGGGGAGAGGGGCTGGTTGCTTTGGAGCATACTCAAGTTCAAACCCCAAATCTTGCTCGGTGAACTCGAAAGATGAGGGATTTGAATTCAGAGGTGATGACAATCTTCTGACTGTGACAATTCCTGCCGCAACTAGTTCTTGGAACATTAGTTTCTCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

807

Amino Acids

88.97

Weight (kDa)

6.51

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 73 - 793 1.4e-261 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 217, 1358
AccB1I GGYRCC 1 cut(s) 317
AciI CCGC 3 cut(s) 621, 1689, 2388
AclWI GGATC 3 cut(s) 110, 1253, 1811
AcoI YGGCCR 1 cut(s) 1714
AcsI RAATTY 6 cut(s) 554, 1066, 1195, 1855, 2103, 2345
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 6 cut(s) 442, 517, 787, 862, 1182, 2192
AfiI CCNNNNNNNGG 5 cut(s) 434, 1621, 1645, 1922, 2140
AflIII ACRYGT 3 cut(s) 247, 1626, 2187
AhdI GACNNNNNGTC 1 cut(s) 1931
AhlI ACTAGT 2 cut(s) 164, 2393
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 6 cut(s) 757, 1141, 1189, 1711, 1783, 1935
AloI GAACNNNNNNTCC 2 cut(s) 1113, 1145
Alw26I GTCTC 7 cut(s) 175, 825, 1043, 1349, 1390, 1975, 2067
AlwI GGATC 3 cut(s) 110, 1253, 1811
AlwNI CAGNNNCTG 1 cut(s) 1561
AoxI GGCC 7 cut(s) 30, 835, 1075, 1468, 1714, 1790, 1913
ApeKI GCWGC 8 cut(s) 305, 564, 999, 1461, 1571, 1649, 1664, 1667
ApoI RAATTY 6 cut(s) 554, 1066, 1195, 1855, 2103, 2345
ArsI GACNNNNNNTTYG 4 cut(s) 2066, 2069, 2098, 2101
AspLEI GCGC 1 cut(s) 320
AspS9I GGNCC 2 cut(s) 31, 1468
AsuHPI GGTGA 7 cut(s) 563, 626, 700, 1179, 1613, 2332, 2366
AxyI CCTNAGG 1 cut(s) 1079
BaeI ACNNNNGTAYC 2 cut(s) 2039, 2072
BalI TGGCCA 1 cut(s) 1716
BanI GGYRCC 1 cut(s) 317
BarI GAAGNNNNNNTAC 2 cut(s) 1495, 1527
BauI CACGAG 2 cut(s) 429, 1515
BbsI GAAGAC 3 cut(s) 271, 710, 1832
BbvI GCAGC 8 cut(s) 317, 576, 1011, 1448, 1558, 1651, 1654, 1661
BccI CCATC 9 cut(s) 122, 171, 542, 681, 884, 1208, 1316, 1751, 1801
BceAI ACGGC 1 cut(s) 850
BcgI CGANNNNNNTGC 2 cut(s) 736, 770
BciT130I CCWGG 6 cut(s) 759, 1143, 1191, 1713, 1785, 1937
BciVI GTATCC 1 cut(s) 2140
BcoDI GTCTC 7 cut(s) 175, 825, 1043, 1349, 1390, 1975, 2067
BcuI ACTAGT 2 cut(s) 164, 2393
BfmI CTRYAG 3 cut(s) 624, 1572, 1633
BfoI RGCGCY 1 cut(s) 321
BfuAI ACCTGC 2 cut(s) 217, 1358
BfuI GTATCC 1 cut(s) 2140
BisI GCNGC 9 cut(s) 306, 565, 1000, 1462, 1572, 1650, 1665, 1668, 2388
BlsI GCNGC 9 cut(s) 307, 566, 1001, 1463, 1573, 1651, 1666, 1669, 2389
Bme1390I CCNGG 6 cut(s) 759, 1143, 1191, 1713, 1785, 1937
BmeRI GACNNNNNGTC 1 cut(s) 1931
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 2 cut(s) 31, 1468
BmiI GGNNCC 4 cut(s) 133, 319, 671, 933
BmrFI CCNGG 6 cut(s) 759, 1143, 1191, 1713, 1785, 1937
BmrI ACTGGG 1 cut(s) 1172
BmsI GCATC 4 cut(s) 744, 784, 1529, 2224
BmuI ACTGGG 1 cut(s) 1172
BpiI GAAGAC 3 cut(s) 271, 710, 1832
BplI GAGNNNNNCTC 2 cut(s) 822, 854
BpmI CTGGAG 4 cut(s) 849, 1125, 1373, 2043
BpuEI CTTGAG 4 cut(s) 614, 1025, 1436, 2278
Bsa29I ATCGAT 1 cut(s) 885
BsaAI YACGTR 1 cut(s) 377
BsaBI GATNNNNATC 1 cut(s) 2361
BsaHI GRCGYC 1 cut(s) 318
BsaI GGTCTC 2 cut(s) 1043, 1975
BsaJI CCNNGG 5 cut(s) 27, 313, 1597, 1784, 1897
BsaWI WCCGGW 1 cut(s) 2198
BsaXI ACNNNNNCTCC 8 cut(s) 1113, 1143, 1368, 1398, 1683, 1713, 2091, 2121
Bsc4I CCNNNNNNNGG 5 cut(s) 434, 1621, 1645, 1922, 2140
Bse118I RCCGGY 1 cut(s) 1638
Bse1I ACTGG 5 cut(s) 539, 1178, 1356, 1820, 2026
Bse21I CCTNAGG 1 cut(s) 1079
Bse3DI GCAATG 1 cut(s) 1566
Bse8I GATNNNNATC 1 cut(s) 2361
BseBI CCWGG 6 cut(s) 759, 1143, 1191, 1713, 1785, 1937
BseCI ATCGAT 1 cut(s) 885
BseDI CCNNGG 5 cut(s) 27, 313, 1597, 1784, 1897
BseGI GGATG 9 cut(s) 182, 426, 453, 553, 581, 673, 799, 1219, 1321
BseJI GATNNNNATC 1 cut(s) 2361
BseLI CCNNNNNNNGG 5 cut(s) 434, 1621, 1645, 1922, 2140
BseMI GCAATG 1 cut(s) 1566
BseMII CTCAG 7 cut(s) 221, 482, 648, 853, 1047, 1644, 1783
BseNI ACTGG 5 cut(s) 539, 1178, 1356, 1820, 2026
BseRI GAGGAG 6 cut(s) 106, 109, 112, 509, 512, 1967
BseXI GCAGC 8 cut(s) 317, 576, 1011, 1448, 1558, 1651, 1654, 1661
BsgI GTGCAG 2 cut(s) 1547, 2052
BshFI GGCC 7 cut(s) 32, 837, 1077, 1470, 1716, 1792, 1915
BshNI GGYRCC 1 cut(s) 317
BshVI ATCGAT 1 cut(s) 885
BsiSI CCGG 2 cut(s) 1639, 2199
BslFI GGGAC 4 cut(s) 961, 1598, 1637, 1938
BslI CCNNNNNNNGG 5 cut(s) 434, 1621, 1645, 1922, 2140
BsmAI GTCTC 7 cut(s) 175, 825, 1043, 1349, 1390, 1975, 2067
BsmBI CGTCTC 1 cut(s) 825
BsmFI GGGAC 4 cut(s) 961, 1598, 1637, 1938
BsmI GAATGC 2 cut(s) 308, 768
BsnI GGCC 7 cut(s) 32, 837, 1077, 1470, 1716, 1792, 1915
Bso31I GGTCTC 2 cut(s) 1043, 1975
Bsp1407I TGTACA 1 cut(s) 2190
Bsp143I GATC 6 cut(s) 102, 682, 1059, 1258, 1288, 1816
BspACI CCGC 3 cut(s) 621, 1689, 2388
BspANI GGCC 7 cut(s) 32, 837, 1077, 1470, 1716, 1792, 1915
BspCNI CTCAG 7 cut(s) 222, 481, 647, 852, 1048, 1645, 1782
BspDI ATCGAT 1 cut(s) 885
BspLI GGNNCC 4 cut(s) 133, 319, 671, 933
BspMAI CTGCAG 1 cut(s) 1576
BspMI ACCTGC 2 cut(s) 217, 1358
BspPI GGATC 3 cut(s) 110, 1253, 1811
BspT107I GGYRCC 1 cut(s) 317
BspTNI GGTCTC 2 cut(s) 1043, 1975
BsrDI GCAATG 1 cut(s) 1566
BsrFI RCCGGY 1 cut(s) 1638
BsrGI TGTACA 1 cut(s) 2190
BsrI ACTGG 5 cut(s) 539, 1178, 1356, 1820, 2026
BssAI RCCGGY 1 cut(s) 1638
BssECI CCNNGG 5 cut(s) 27, 313, 1597, 1784, 1897
BssMI GATC 6 cut(s) 102, 682, 1059, 1258, 1288, 1816
BssNI GRCGYC 1 cut(s) 318
BssSI CACGAG 2 cut(s) 429, 1515
BssT1I CCWWGG 4 cut(s) 27, 313, 1597, 1897
Bst2BI CACGAG 2 cut(s) 429, 1515
Bst2UI CCWGG 6 cut(s) 759, 1143, 1191, 1713, 1785, 1937
Bst4CI ACNGT 8 cut(s) 16, 954, 1185, 1360, 1765, 2128, 2216, 2374
Bst6I CTCTTC 3 cut(s) 61, 1829, 1992
BstACI GRCGYC 1 cut(s) 318
BstAPI GCANNNNNTGC 1 cut(s) 1458
BstAUI TGTACA 1 cut(s) 2190
BstBAI YACGTR 1 cut(s) 377
BstC8I GCNNGC 5 cut(s) 569, 1101, 1640, 1718, 1913
BstEII GGTNACC 1 cut(s) 614
BstF5I GGATG 9 cut(s) 182, 426, 453, 553, 581, 673, 799, 1219, 1321
BstH2I RGCGCY 1 cut(s) 321
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 6 cut(s) 105, 685, 1062, 1261, 1291, 1819
BstMAI GTCTC 7 cut(s) 175, 825, 1043, 1349, 1390, 1975, 2067
BstMBI GATC 6 cut(s) 102, 682, 1059, 1258, 1288, 1816
BstMWI GCNNNNNNNGC 6 cut(s) 629, 763, 1458, 1467, 1577, 1673
BstNI CCWGG 6 cut(s) 759, 1143, 1191, 1713, 1785, 1937
BstNSI RCATGY 4 cut(s) 251, 1103, 1630, 2191
BstPI GGTNACC 1 cut(s) 614
BstSCI CCNGG 6 cut(s) 757, 1141, 1189, 1711, 1783, 1935
BstSFI CTRYAG 3 cut(s) 624, 1572, 1633
BstV1I GCAGC 8 cut(s) 317, 576, 1011, 1448, 1558, 1651, 1654, 1661
BstV2I GAAGAC 3 cut(s) 271, 710, 1832
BstX2I RGATCY 1 cut(s) 1258
BstYI RGATCY 1 cut(s) 1258
Bsu15I ATCGAT 1 cut(s) 885
Bsu36I CCTNAGG 1 cut(s) 1079
BsuI GTATCC 1 cut(s) 2140
BsuRI GGCC 7 cut(s) 32, 837, 1077, 1470, 1716, 1792, 1915
BsuTUI ATCGAT 1 cut(s) 885
BtgZI GCGATG 1 cut(s) 2130
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 9 cut(s) 182, 426, 453, 553, 581, 673, 799, 1219, 1321
BtsI GCAGTG 2 cut(s) 1559, 1581
BtsIMutI CAGTG 4 cut(s) 244, 1559, 1581, 2236
BveI ACCTGC 2 cut(s) 217, 1358
Cac8I GCNNGC 5 cut(s) 569, 1101, 1640, 1718, 1913
CaiI CAGNNNCTG 1 cut(s) 1561
CfoI GCGC 1 cut(s) 320
Cfr10I RCCGGY 1 cut(s) 1638
Cfr13I GGNCC 2 cut(s) 31, 1468
ClaI ATCGAT 1 cut(s) 885
Csp6I GTAC 6 cut(s) 441, 516, 786, 861, 1181, 2191
CspCI CAANNNNNGTGG 4 cut(s) 1015, 1050, 2182, 2217
CviQI GTAC 6 cut(s) 441, 516, 786, 861, 1181, 2191
DinI GGCGCC 1 cut(s) 319
DpnI GATC 6 cut(s) 104, 684, 1061, 1260, 1290, 1818
DpnII GATC 6 cut(s) 102, 682, 1059, 1258, 1288, 1816
DraI TTTAAA 1 cut(s) 409
DriI GACNNNNNGTC 1 cut(s) 1931
EaeI YGGCCR 1 cut(s) 1714
Eam1104I CTCTTC 3 cut(s) 61, 1829, 1992
Eam1105I GACNNNNNGTC 1 cut(s) 1931
EarI CTCTTC 3 cut(s) 61, 1829, 1992
Eco130I CCWWGG 4 cut(s) 27, 313, 1597, 1897
Eco31I GGTCTC 2 cut(s) 1043, 1975
Eco32I GATATC 3 cut(s) 1301, 1781, 1984
Eco81I CCTNAGG 1 cut(s) 1079
Eco91I GGTNACC 1 cut(s) 614
EcoO109I RGGNCCY 1 cut(s) 31
EcoO65I GGTNACC 1 cut(s) 614
EcoRI GAATTC 2 cut(s) 1066, 2345
EcoRII CCWGG 6 cut(s) 757, 1141, 1189, 1711, 1783, 1935
EcoRV GATATC 3 cut(s) 1301, 1781, 1984
EcoT14I CCWWGG 4 cut(s) 27, 313, 1597, 1897
EgeI GGCGCC 1 cut(s) 319
EheI GGCGCC 1 cut(s) 319
ErhI CCWWGG 4 cut(s) 27, 313, 1597, 1897
Esp3I CGTCTC 1 cut(s) 825
FalI AAGNNNNNCTT 4 cut(s) 1055, 1087, 1900, 1932
FaqI GGGAC 4 cut(s) 961, 1598, 1637, 1938
FauI CCCGC 1 cut(s) 628
Fnu4HI GCNGC 9 cut(s) 306, 565, 1000, 1462, 1572, 1650, 1665, 1668, 2388
FokI GGATG 9 cut(s) 189, 433, 440, 560, 568, 660, 806, 1226, 1328
Fsp4HI GCNGC 9 cut(s) 306, 565, 1000, 1462, 1572, 1650, 1665, 1668, 2388
GlaI GCGC 1 cut(s) 319
GluI GCNGC 9 cut(s) 306, 565, 1000, 1462, 1572, 1650, 1665, 1668, 2388
GsuI CTGGAG 4 cut(s) 849, 1125, 1373, 2043
HaeII RGCGCY 1 cut(s) 321
HaeIII GGCC 7 cut(s) 32, 837, 1077, 1470, 1716, 1792, 1915
HapII CCGG 2 cut(s) 1639, 2199
HhaI GCGC 1 cut(s) 320
Hin1I GRCGYC 1 cut(s) 318
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HincII GTYRAC 3 cut(s) 255, 814, 2212
HindII GTYRAC 3 cut(s) 255, 814, 2212
HindIII AAGCTT 2 cut(s) 727, 2012
HinfI GANTC 9 cut(s) 221, 369, 638, 695, 1383, 1401, 1481, 1692, 1837
HpaI GTTAAC 1 cut(s) 814
HpaII CCGG 2 cut(s) 1639, 2199
HphI GGTGA 7 cut(s) 563, 626, 700, 1179, 1613, 2332, 2366
Hpy188III TCNNGA 5 cut(s) 545, 1063, 1729, 1754, 2007
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 7 cut(s) 44, 848, 920, 938, 1960, 2053, 2068
HpyCH4III ACNGT 8 cut(s) 16, 954, 1185, 1360, 1765, 2128, 2216, 2374
HpyCH4IV ACGT 2 cut(s) 261, 376
HpyCH4V TGCA 9 cut(s) 571, 1265, 1464, 1520, 1564, 1574, 1869, 2033, 2168
HpyF10VI GCNNNNNNNGC 6 cut(s) 629, 763, 1458, 1467, 1577, 1673
HpySE526I ACGT 2 cut(s) 261, 376
Hsp92I GRCGYC 1 cut(s) 318
HspAI GCGC 1 cut(s) 318
KasI GGCGCC 1 cut(s) 317
KroI GCCGGC 1 cut(s) 1638
KroNI GCCGGC 1 cut(s) 1640
KspAI GTTAAC 1 cut(s) 814
Kzo9I GATC 6 cut(s) 102, 682, 1059, 1258, 1288, 1816
LmnI GCTCC 8 cut(s) 483, 931, 1144, 1217, 1901, 1954, 2204, 2285
Lsp1109I GCAGC 8 cut(s) 317, 576, 1011, 1448, 1558, 1651, 1654, 1661
LweI GCATC 4 cut(s) 744, 784, 1529, 2224
MaeII ACGT 2 cut(s) 261, 376
MaeIII GTNAC 7 cut(s) 449, 614, 1022, 1185, 1703, 2092, 2374
MalI GATC 6 cut(s) 104, 684, 1061, 1260, 1290, 1818
MboI GATC 6 cut(s) 102, 682, 1059, 1258, 1288, 1816
MfeI CAATTG 1 cut(s) 1891
MflI RGATCY 1 cut(s) 1258
MlsI TGGCCA 1 cut(s) 1716
MluNI TGGCCA 1 cut(s) 1716
Mly113I GGCGCC 1 cut(s) 318
MlyI GAGTC 5 cut(s) 378, 1392, 1475, 1701, 1846
MmeI TCCRAC 1 cut(s) 1429
Mox20I TGGCCA 1 cut(s) 1716
MroNI GCCGGC 1 cut(s) 1638
MscI TGGCCA 1 cut(s) 1716
MseI TTAA 9 cut(s) 150, 360, 392, 408, 813, 968, 1329, 1737, 2139
MslI CAYNNNNRTG 1 cut(s) 458
Msp20I TGGCCA 1 cut(s) 1716
MspA1I CMGCKG 2 cut(s) 472, 1652
MspI CCGG 2 cut(s) 1639, 2199
MspR9I CCNGG 6 cut(s) 759, 1143, 1191, 1713, 1785, 1937
MunI CAATTG 1 cut(s) 1891
Mva1269I GAATGC 2 cut(s) 308, 768
MvaI CCWGG 6 cut(s) 759, 1143, 1191, 1713, 1785, 1937
MwoI GCNNNNNNNGC 6 cut(s) 629, 763, 1458, 1467, 1577, 1673
NaeI GCCGGC 1 cut(s) 1640
NarI GGCGCC 1 cut(s) 318
NdeII GATC 6 cut(s) 102, 682, 1059, 1258, 1288, 1816
NgoMIV GCCGGC 1 cut(s) 1638
NlaIV GGNNCC 4 cut(s) 133, 319, 671, 933
NmeAIII GCCGAG 1 cut(s) 27
NmuCI GTSAC 7 cut(s) 449, 614, 1022, 1185, 1703, 2092, 2374
NspI RCATGY 4 cut(s) 251, 1103, 1630, 2191
PaeI GCATGC 1 cut(s) 1103
PciI ACATGT 3 cut(s) 247, 1626, 2187
PcsI WCGNNNNNNNCGW 1 cut(s) 2324
PctI GAATGC 2 cut(s) 308, 768
PdiI GCCGGC 1 cut(s) 1640
PfeI GAWTC 4 cut(s) 221, 638, 695, 1401
PfoI TCCNGGA 1 cut(s) 1141
PkrI GCNGC 9 cut(s) 307, 566, 1001, 1463, 1573, 1651, 1666, 1669, 2389
PleI GAGTC 5 cut(s) 377, 1391, 1475, 1700, 1845
PluTI GGCGCC 1 cut(s) 321
PpsI GAGTC 5 cut(s) 377, 1391, 1475, 1700, 1845
Ppu21I YACGTR 1 cut(s) 377
PscI ACATGT 3 cut(s) 247, 1626, 2187
Psp6I CCWGG 6 cut(s) 757, 1141, 1189, 1711, 1783, 1935
PspEI GGTNACC 1 cut(s) 614
PspGI CCWGG 6 cut(s) 757, 1141, 1189, 1711, 1783, 1935
PspN4I GGNNCC 4 cut(s) 133, 319, 671, 933
PspPI GGNCC 2 cut(s) 31, 1468
PstI CTGCAG 1 cut(s) 1576
PstNI CAGNNNCTG 1 cut(s) 1561
PsuI RGATCY 1 cut(s) 1258
PvuII CAGCTG 2 cut(s) 472, 1652
RsaI GTAC 6 cut(s) 442, 517, 787, 862, 1182, 2192
RsaNI GTAC 6 cut(s) 441, 516, 786, 861, 1181, 2191
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 9 cut(s) 150, 360, 392, 408, 813, 968, 1329, 1737, 2139
SatI GCNGC 9 cut(s) 306, 565, 1000, 1462, 1572, 1650, 1665, 1668, 2388
Sau3AI GATC 6 cut(s) 102, 682, 1059, 1258, 1288, 1816
Sau96I GGNCC 2 cut(s) 31, 1468
SchI GAGTC 5 cut(s) 378, 1392, 1475, 1701, 1846
ScrFI CCNGG 6 cut(s) 759, 1143, 1191, 1713, 1785, 1937
SfaNI GCATC 4 cut(s) 744, 784, 1529, 2224
SfcI CTRYAG 3 cut(s) 624, 1572, 1633
SfoI GGCGCC 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 4 cut(s) 593, 1040, 1415, 2293
SmoI CTYRAG 4 cut(s) 593, 1040, 1415, 2293
SpeI ACTAGT 2 cut(s) 164, 2393
SphI GCATGC 1 cut(s) 1103
SsiI CCGC 3 cut(s) 621, 1689, 2388
SspDI GGCGCC 1 cut(s) 317
SspI AATATT 1 cut(s) 2158
StyD4I CCNGG 6 cut(s) 757, 1141, 1189, 1711, 1783, 1935
StyI CCWWGG 4 cut(s) 27, 313, 1597, 1897
TaaI ACNGT 8 cut(s) 16, 954, 1185, 1360, 1765, 2128, 2216, 2374
TaiI ACGT 2 cut(s) 264, 379
TaqI TCGA 6 cut(s) 606, 681, 885, 1728, 1986, 2327
TatI WGTACW 5 cut(s) 440, 515, 785, 1180, 2190
TauI GCSGC 1 cut(s) 2390
TfiI GAWTC 4 cut(s) 221, 638, 695, 1401
Tru1I TTAA 9 cut(s) 150, 360, 392, 408, 813, 968, 1329, 1737, 2139
Tru9I TTAA 9 cut(s) 150, 360, 392, 408, 813, 968, 1329, 1737, 2139
TscAI CASTG 4 cut(s) 244, 1566, 1581, 2236
TseFI GTSAC 7 cut(s) 449, 614, 1022, 1185, 1703, 2092, 2374
TseI GCWGC 8 cut(s) 305, 564, 999, 1461, 1571, 1649, 1664, 1667
Tsp45I GTSAC 7 cut(s) 449, 614, 1022, 1185, 1703, 2092, 2374
TspDTI ATGAA 4 cut(s) 48, 218, 603, 716
TspGWI ACGGA 3 cut(s) 124, 1311, 1887
TspRI CASTG 4 cut(s) 244, 1566, 1581, 2236
XapI RAATTY 6 cut(s) 554, 1066, 1195, 1855, 2103, 2345
XceI RCATGY 4 cut(s) 251, 1103, 1630, 2191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.