Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0000881

Physical Location & Seq

Genomic Coordinates Reverse (-)

22425226 .. 22430824

Gene Structure 5599 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00400310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGACTGTCACTCCAAACATTTCCATCAACGATGGAAACCTGGTGGTTCACGGAAAGACCATATTGACCGGAGTGCCGGAAAACATTGTGCTCACTCCAGGCTCCGGTGTCGGCCTCGTAGCTGGTGCTTTCATTGGCGCCACGGCCTCTCACAGCAAAAGCCTCCATGTCTTCCCTGTCGGTGTTTTAGAGGGTCAGCGGTTCATGTGCCTTTTCCGGTTTAAGCTATGGTGGATGACGCAGAGGATGGGGTCATGTGGGAAAGAAGTTCCGCTGGAGACCCAATTCATGCTTGTGGAGACCAAAGATGATGGTGAAGGAAGTGATGAATCCTCTACCATTTACACAGTTTTCCTTCCTCTGCTGGAGGGCCAGTTCCGTTCGGTTCTGCAAGGGAACGAGCGGAATGAAGTGGAGATATGCCTCGAGAGTGGAGATTCTGATGTTCAGACCAATCAAGGTCTTTGCCTTGTTTACGTGCATGCTGGAACAAATCCATTTGAAGTTATCAACCAGGCTGTAAAGGCTGTAGAAAAACACATGCAAACCTTTGTCCATCGTGAGAAGAAAAAGTTGCCTTCTTTCCTCGACTGGTTTGGCTGGTGCACATGGGATGCTTTCTATACCGATGTCACAGCTGAGGGTGTCGTGGATGGTCTTAAAAGCTTGAATGAGGGAGGGACGCCTCCGCGATTTCTAATCGTAGATGATGGTTGGCAACAAATTGAAAGTAAACCCAAGGATACTAATGTTGCAGTACAAGAAGGAGCACAGTTTGCAAGTAGGCTTACAGGAATAAGAGAGAATGAAAAATTCCAAAAGAATGGACAGAGTGAGCAAGTCTCAGGCCTGAAACATGTTGTTGATGAAGCGAAGCAGCATCACAATGTGAAGTTTGTGTATGTATGGCATGCCTTAGTTGGGTACTGGGGTGGAGTGAAACCAGCTGCTTCTGGCATGGAGCATTATGACACTGCTTTGGCATATCCAGTCTCATCTCCTGGTGTGATGGGAAACCAACCTGACATAGTTTTTGACAGCTTATCTGTTCACGGTCTTGGTTTGGTGCATCCAAAGAAAGTTTTCAATTTCTACAATGAGCTTCATGCGTATTTGTCTTCCTGTGGAGTGGATGGAGTTAAGGTTGATGTACAGAACATCATTGAGACTCTTGGGTCTGGTCATGGTGGCAGAGTTTCTCTGACCCGAAGCTATCACCAGGCACTTGAGGCTTCGGTTGCTCGGAACTTTCCTGACAACGGATGCATTTCTTGCATGTGTCATAACACTGATGGGCTATATAGTTCCAAGCAGACTGCTGTTGTGAGAGCTTCTGATGACTTCTACCCACGGGATCCTGCTTCTCACACGATTCATATATCTTCTGTGGCTTATAACACCCTTTTCCTTGGCGAGTTTATGCAACCAGACTGGGATATGTTTCATAGTTTACATCCAGCAGCGGATTATCATGGTGCTGCTCGTTCTATTGGTGGATGTGCAATCTATGTCAGTGATAAACCTGGAAACCACAACTTCGATCTTTTGAGGAAGCTGGTCCTGCCTGATGGATCTGTCCTCCGTGCTCAATTACCTGGTAGGCCAACATGTGATTGTCTCTTTGCTGACCCAGCAAGAGATGGAACCAGCTTGCTCAAGATATGGAATGTGAACAAGTGCTCTGGTGTTGTTGGTGTATTCAACTGTCAAGGGGCTGGTTGGTGCAAGGTTGAAAAGAAGACCCGCATCCATGATGCATCTCCTGGTACCCTCACTGGTTCTGTTCGTGCTATTGATGTTGATAGCCTTGCCCAAATTGCTGGTGATGATTGGAATGGAGAAACAGTAGTTTATGCCCATAAGTCAGGAGAGGTGTTTCGTTTACCAAAAGATGTATCAGTGCCAGTGACACTTAAAGTTCTGGAGTATGAGCTCTTCCATTTCTGTCCTCTCAAGGAGGTTACACCCAACATCTCATTTGCTCCAATAGGCCTGCTTGACATGTTCAACTCCAGTGGTGCTGTGGAGCATGTTGTAATCCATCTGGCTTCTGACGGCGAAGTTTCTTCAGAGCTTACCACTTCTCTCAGTGAGGATAGATCTTCAACTGCCACAATCACCATCAAAACCAGAGGATGTGGAAGGTTTGGAGCTTACTCTTCTCAGCGTCCCCTGAAGTGTACCGTTGATAGAGTTGAGACGGACTTTGAATATGACCCTGCAACTGGATTACTAACCTTTACCATCCCTATCCCAAAAGAGGATATGTATAAATGGCTAGTAGAAATCCAAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005911 GO:0005975 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009506 GO:0009628 GO:0015925 GO:0016052 GO:0016787 GO:0016798 GO:0030054 GO:0033530 GO:0034484 GO:0044238 GO:0050896 GO:0052692 GO:0055044 GO:0071704 GO:0080167 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00052 map00052

Pfam Domains

Protein Families

Raffinose_syn

Protein Analysis

765

Amino Acids

83.85

Weight (kDa)

5.63

Isoelectric Point (pI)

31.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Raffinose_syn	PF05691	7 - 748	0.0	Raffinose synthase or seed imbibition protein Sip1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57520 AT3G57520 AT3G57520
fragaria_vesca	FvH4_3g00160 FvH4_6g23550 FvH4_6g23550 FvH4_6g23550 FvH4_6g23550
malus_domestica	MD09G1161700.v1.1 MD09G1162100.v1.1 MD10G1341400.v1.1 MD10G1341500.v1.1 MD10G1341600.v1.1 MD12G1077800.v1.1 MD14G1070300.v1.1
prunus_persica	Prupe.4G001700_v2.0.a1 Prupe.5G162400_v2.0.a1 Prupe.5G162400_v2.0.a1 Prupe.7G064000_v2.0.a1 Prupe.7G064000_v2.0.a1
pyrus_communis	pycom10g29090 pycom12g06210 pycom14g05800 pycom14g05880 pycom14g05890 pycom14g05910 pycom14g05930
rosa_chinensis	RchiOBHm_Chr3g0481621 RchiOBHm_Chr5g0000211 RchiOBHm_Chr7g0192011 RchiOBHm_Chr7g0192021
rosa_laevigata	RLG00000004409 RLG00000023385 RLG00000030850
rosa_multiflora	Rmu_co8093432.1_g000001 Rmu_co8314355.1_g000001 Rmu_sc0010409.1_g000002 Rmu_sc0021936.1_g000002 Rmu_ssc0000243.1_g000010 Rmu_ssc0000421.1_g000033
rosa_roxburghii	Rroxscaffold_1G00075890 Rroxscaffold_3G00263360 Rroxscaffold_6G00400310
rosa_rugosa	Rorug03G0192700 Rorug03G0192800 Rorug03G0192900 Rorug04G0382500 Rorug06G0512300 Rorug06G0512300
rosa_samantha	Rh3AG244100 Rh3BG279100 Rh3BG279300 Rh3CG274100 Rh3DG270900 Rh5AG001400 Rh5BG002800 Rh5CG001700 Rh5DG001600 Rh7AG122700 Rh7AG122800 Rh7BG124300 Rh7CG127000 Rh7DG125800
rosa_wichuraiana	Rw3G021800 Rw5G000190 Rw7G010450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1395
AasI	GACNNNNNNGTC	1 cut(s)	1174
Acc65I	GGTACC	1 cut(s)	1766
AccB1I	GGYRCC	2 cut(s)	137, 1766
AccBSI	CCGCTC	1 cut(s)	403
AccII	CGCG	1 cut(s)	691
AciI	CCGC	6 cut(s)	199, 272, 403, 689, 1463, 1744
AclWI	GGATC	3 cut(s)	1349, 1362, 1579
AcsI	RAATTY	1 cut(s)	812
AcuI	CTGAAG	2 cut(s)	2052, 2195
AcyI	GRCGYC	2 cut(s)	138, 683
AdeI	CACNNNGTG	1 cut(s)	889
AfaI	GTAC	5 cut(s)	759, 926, 1152, 1768, 2182
AfiI	CCNNNNNNNGG	5 cut(s)	104, 921, 1259, 2225, 2260
AflIII	ACRYGT	3 cut(s)	856, 1607, 2001
AgsI	TTSAA	9 cut(s)	503, 670, 728, 1087, 1702, 1733, 2008, 2106, 2210
AjnI	CCWGG	8 cut(s)	39, 97, 513, 1000, 1218, 1522, 1594, 1762
AloI	GAACNNNNNNTCC	2 cut(s)	359, 391
Alw21I	GWGCWC	6 cut(s)	93, 608, 772, 1588, 1682, 1935
Alw26I	GTCTC	7 cut(s)	272, 293, 847, 997, 1160, 1622, 2192
Alw44I	GTGCAC	1 cut(s)	604
AlwI	GGATC	3 cut(s)	1349, 1362, 1579
Ama87I	CYCGRG	1 cut(s)	425
AoxI	GGCC	6 cut(s)	112, 144, 370, 847, 1601, 1990
ApaLI	GTGCAC	1 cut(s)	604
ApeKI	GCWGC	4 cut(s)	877, 947, 1460, 1478
ApoI	RAATTY	1 cut(s)	812
ArsI	GACNNNNNNTTYG	2 cut(s)	1016, 1048
Asp700I	GAANNNNTTC	1 cut(s)	1082
Asp718I	GGTACC	1 cut(s)	1766
AspLEI	GCGC	1 cut(s)	140
AspS9I	GGNCC	2 cut(s)	370, 1558
AsuHPI	GGTGA	4 cut(s)	326, 1208, 1835, 2110
AvaI	CYCGRG	1 cut(s)	425
AvaII	GGWCC	1 cut(s)	1558
BaeGI	GKGCMC	1 cut(s)	608
BaeI	ACNNNNGTAYC	4 cut(s)	735, 768, 916, 949
BamHI	GGATCC	1 cut(s)	1354
BanI	GGYRCC	2 cut(s)	137, 1766
BanII	GRGCYC	1 cut(s)	1935
BarI	GAAGNNNNNNTAC	2 cut(s)	1919, 1951
BbsI	GAAGAC	3 cut(s)	163, 1110, 1745
Bbv12I	GWGCWC	6 cut(s)	93, 608, 772, 1588, 1682, 1935
BbvCI	CCTCAGC	1 cut(s)	639
BbvI	GCAGC	4 cut(s)	889, 934, 1465, 1472
BceAI	ACGGC	2 cut(s)	159, 2071
BciT130I	CCWGG	8 cut(s)	41, 99, 515, 1002, 1220, 1524, 1596, 1764
BciVI	GTATCC	1 cut(s)	736
BcoDI	GTCTC	7 cut(s)	272, 293, 847, 997, 1160, 1622, 2192
BfaI	CTAG	1 cut(s)	2279
BfmI	CTRYAG	1 cut(s)	528
BfoI	RGCGCY	1 cut(s)	141
BfuI	GTATCC	1 cut(s)	736
BglII	AGATCT	1 cut(s)	2099
BisI	GCNGC	4 cut(s)	878, 948, 1461, 1479
BlsI	GCNGC	4 cut(s)	879, 949, 1462, 1480
Bme1390I	CCNGG	8 cut(s)	41, 99, 515, 1002, 1220, 1524, 1596, 1764
Bme18I	GGWCC	1 cut(s)	1558
BmeT110I	CYCGRG	1 cut(s)	425
BmgT120I	GGNCC	2 cut(s)	370, 1558
BmiI	GGNNCC	5 cut(s)	103, 139, 1356, 1645, 1768
BmrFI	CCNGG	8 cut(s)	41, 99, 515, 1002, 1220, 1524, 1596, 1764
BmrI	ACTGGG	2 cut(s)	937, 1441
BmsI	GCATC	7 cut(s)	604, 889, 1078, 1253, 1744, 1755, 1766
BmuI	ACTGGG	2 cut(s)	937, 1441
BpiI	GAAGAC	3 cut(s)	163, 1110, 1745
BplI	GAGNNNNNCTC	2 cut(s)	827, 859
BpmI	CTGGAG	5 cut(s)	81, 296, 386, 1943, 1996
Bpu10I	CCTNAGC	1 cut(s)	639
BpuEI	CTTGAG	3 cut(s)	1247, 1640, 1937
BsaAI	YACGTR	1 cut(s)	478
BsaHI	GRCGYC	2 cut(s)	138, 683
BsaI	GGTCTC	2 cut(s)	272, 293
BsaJI	CCNNGG	4 cut(s)	141, 738, 1349, 1408
BsaWI	WCCGGW	3 cut(s)	68, 104, 216
BsaXI	ACNNNNNCTCC	7 cut(s)	25, 359, 389, 1128, 1158, 1830, 1860
Bsc4I	CCNNNNNNNGG	5 cut(s)	104, 921, 1259, 2225, 2260
Bse1I	ACTGG	9 cut(s)	373, 596, 932, 989, 1436, 1780, 1904, 2013, 2230
BseBI	CCWGG	8 cut(s)	41, 99, 515, 1002, 1220, 1524, 1596, 1764
BseDI	CCNNGG	4 cut(s)	141, 738, 1349, 1408
BseLI	CCNNNNNNNGG	5 cut(s)	104, 921, 1259, 2225, 2260
BseMII	CTCAG	4 cut(s)	630, 858, 2101, 2177
BseNI	ACTGG	9 cut(s)	373, 596, 932, 989, 1436, 1780, 1904, 2013, 2230
BseSI	GKGCMC	1 cut(s)	608
BseXI	GCAGC	4 cut(s)	889, 934, 1465, 1472
BseYI	CCCAGC	1 cut(s)	1630
Bsh1236I	CGCG	1 cut(s)	691
BshFI	GGCC	6 cut(s)	114, 146, 372, 849, 1603, 1992
BshNI	GGYRCC	2 cut(s)	137, 1766
BsiHKAI	GWGCWC	6 cut(s)	93, 608, 772, 1588, 1682, 1935
BsiHKCI	CYCGRG	1 cut(s)	425
BsiSI	CCGG	4 cut(s)	69, 77, 105, 217
BslFI	GGGAC	2 cut(s)	694, 2154
BslI	CCNNNNNNNGG	5 cut(s)	104, 921, 1259, 2225, 2260
BsmAI	GTCTC	7 cut(s)	272, 293, 847, 997, 1160, 1622, 2192
BsmBI	CGTCTC	1 cut(s)	2192
BsmFI	GGGAC	2 cut(s)	694, 2154
BsnI	GGCC	6 cut(s)	114, 146, 372, 849, 1603, 1992
Bso31I	GGTCTC	2 cut(s)	272, 293
BsoBI	CYCGRG	1 cut(s)	425
Bsp1286I	GDGCHC	6 cut(s)	93, 608, 772, 1588, 1682, 1935
Bsp1407I	TGTACA	1 cut(s)	1150
Bsp143I	GATC	4 cut(s)	1354, 1540, 1571, 2099
BspACI	CCGC	6 cut(s)	199, 272, 403, 689, 1463, 1744
BspANI	GGCC	6 cut(s)	114, 146, 372, 849, 1603, 1992
BspCNI	CTCAG	4 cut(s)	631, 857, 2100, 2176
BspFNI	CGCG	1 cut(s)	691
BspLI	GGNNCC	5 cut(s)	103, 139, 1356, 1645, 1768
BspPI	GGATC	3 cut(s)	1349, 1362, 1579
BspQI	GCTCTTC	1 cut(s)	1940
BspT107I	GGYRCC	2 cut(s)	137, 1766
BspTNI	GGTCTC	2 cut(s)	272, 293
BsrBI	CCGCTC	1 cut(s)	403
BsrGI	TGTACA	1 cut(s)	1150
BsrI	ACTGG	9 cut(s)	373, 596, 932, 989, 1436, 1780, 1904, 2013, 2230
BssECI	CCNNGG	4 cut(s)	141, 738, 1349, 1408
BssMI	GATC	4 cut(s)	1354, 1540, 1571, 2099
BssNI	GRCGYC	2 cut(s)	138, 683
BssT1I	CCWWGG	2 cut(s)	738, 1408
Bst2UI	CCWGG	8 cut(s)	41, 99, 515, 1002, 1220, 1524, 1596, 1764
Bst4CI	ACNGT	7 cut(s)	7, 349, 774, 1055, 1706, 1846, 2185
Bst6I	CTCTTC	2 cut(s)	1940, 2164
BstACI	GRCGYC	2 cut(s)	138, 683
BstAPI	GCANNNNNTGC	2 cut(s)	776, 1272
BstAUI	TGTACA	1 cut(s)	1150
BstBAI	YACGTR	1 cut(s)	478
BstC8I	GCNNGC	4 cut(s)	483, 912, 1652, 1994
BstDEI	CTNAG	5 cut(s)	639, 844, 916, 2087, 2163
BstDSI	CCRYGG	2 cut(s)	141, 1349
BstFNI	CGCG	1 cut(s)	691
BstH2I	RGCGCY	1 cut(s)	141
BstHHI	GCGC	1 cut(s)	140
BstKTI	GATC	4 cut(s)	1357, 1543, 1574, 2102
BstMAI	GTCTC	7 cut(s)	272, 293, 847, 997, 1160, 1622, 2192
BstMBI	GATC	4 cut(s)	1354, 1540, 1571, 2099
BstMWI	GCNNNNNNNGC	8 cut(s)	524, 776, 1229, 1238, 1272, 1561, 1631, 1817
BstNI	CCWGG	8 cut(s)	41, 99, 515, 1002, 1220, 1524, 1596, 1764
BstNSI	RCATGY	8 cut(s)	485, 544, 860, 914, 1279, 1611, 2005, 2033
BstSCI	CCNGG	8 cut(s)	39, 97, 513, 1000, 1218, 1522, 1594, 1762
BstSFI	CTRYAG	1 cut(s)	528
BstSLI	GKGCMC	1 cut(s)	608
BstUI	CGCG	1 cut(s)	691
BstV1I	GCAGC	4 cut(s)	889, 934, 1465, 1472
BstV2I	GAAGAC	3 cut(s)	163, 1110, 1745
BstX2I	RGATCY	3 cut(s)	1354, 1571, 2099
BstXI	CCANNNNNNTGG	2 cut(s)	824, 1820
BstYI	RGATCY	3 cut(s)	1354, 1571, 2099
BsuI	GTATCC	1 cut(s)	736
BsuRI	GGCC	6 cut(s)	114, 146, 372, 849, 1603, 1992
BtgI	CCRYGG	2 cut(s)	141, 1349
BtsI	GCAGTG	1 cut(s)	972
BtsIMutI	CAGTG	8 cut(s)	972, 1287, 1519, 1773, 1905, 1911, 2020, 2095
Cac8I	GCNNGC	4 cut(s)	483, 912, 1652, 1994
CfoI	GCGC	1 cut(s)	140
Cfr13I	GGNCC	2 cut(s)	370, 1558
CseI	GACGC	3 cut(s)	247, 691, 2156
CsiI	ACCWGGT	2 cut(s)	39, 1594
Csp6I	GTAC	5 cut(s)	758, 925, 1151, 1767, 2181
CspCI	CAANNNNNGTGG	2 cut(s)	1996, 2031
CviQI	GTAC	5 cut(s)	758, 925, 1151, 1767, 2181
DdeI	CTNAG	5 cut(s)	639, 844, 916, 2087, 2163
DinI	GGCGCC	1 cut(s)	139
DpnI	GATC	4 cut(s)	1356, 1542, 1573, 2101
DpnII	GATC	4 cut(s)	1354, 1540, 1571, 2099
DraIII	CACNNNGTG	1 cut(s)	889
DrdI	GACNNNNNNGTC	1 cut(s)	1174
DseDI	GACNNNNNNGTC	1 cut(s)	1174
Eam1104I	CTCTTC	2 cut(s)	1940, 2164
EarI	CTCTTC	2 cut(s)	1940, 2164
Ecl136II	GAGCTC	1 cut(s)	1933
Eco130I	CCWWGG	2 cut(s)	738, 1408
Eco147I	AGGCCT	2 cut(s)	849, 1992
Eco24I	GRGCYC	1 cut(s)	1935
Eco31I	GGTCTC	2 cut(s)	272, 293
Eco47I	GGWCC	1 cut(s)	1558
Eco53kI	GAGCTC	1 cut(s)	1933
Eco57I	CTGAAG	2 cut(s)	2052, 2195
Eco88I	CYCGRG	1 cut(s)	425
EcoICRI	GAGCTC	1 cut(s)	1933
EcoRII	CCWGG	8 cut(s)	39, 97, 513, 1000, 1218, 1522, 1594, 1762
EcoT14I	CCWWGG	2 cut(s)	738, 1408
EcoT22I	ATGCAT	2 cut(s)	1268, 1759
EcoT38I	GRGCYC	1 cut(s)	1935
EgeI	GGCGCC	1 cut(s)	139
EheI	GGCGCC	1 cut(s)	139
ErhI	CCWWGG	2 cut(s)	738, 1408
Esp3I	CGTCTC	1 cut(s)	2192
FaqI	GGGAC	2 cut(s)	694, 2154
FauI	CCCGC	1 cut(s)	1751
Fnu4HI	GCNGC	4 cut(s)	878, 948, 1461, 1479
FriOI	GRGCYC	1 cut(s)	1935
Fsp4HI	GCNGC	4 cut(s)	878, 948, 1461, 1479
FspBI	CTAG	1 cut(s)	2279
GlaI	GCGC	1 cut(s)	139
GluI	GCNGC	4 cut(s)	878, 948, 1461, 1479
GsaI	CCCAGC	1 cut(s)	1634
GsuI	CTGGAG	5 cut(s)	81, 296, 386, 1943, 1996
HaeII	RGCGCY	1 cut(s)	141
HaeIII	GGCC	6 cut(s)	114, 146, 372, 849, 1603, 1992
HapII	CCGG	4 cut(s)	69, 77, 105, 217
HgaI	GACGC	3 cut(s)	247, 691, 2156
HhaI	GCGC	1 cut(s)	140
Hin1I	GRCGYC	2 cut(s)	138, 683
Hin6I	GCGC	1 cut(s)	138
HinP1I	GCGC	1 cut(s)	138
HindIII	AAGCTT	1 cut(s)	664
HinfI	GANTC	4 cut(s)	329, 437, 1168, 1372
HpaII	CCGG	4 cut(s)	69, 77, 105, 217
HphI	GGTGA	4 cut(s)	326, 1208, 1835, 2110
Hpy166II	GTNNAC	9 cut(s)	49, 475, 606, 734, 1051, 1451, 1672, 1883, 2181
Hpy188I	TCNGA	7 cut(s)	442, 450, 1203, 1245, 1336, 2053, 2071
Hpy188III	TCNNGA	6 cut(s)	427, 560, 1253, 1657, 1866, 1922
Hpy8I	GTNNAC	9 cut(s)	49, 475, 606, 734, 1051, 1451, 1672, 1883, 2181
HpyAV	CCTTC	5 cut(s)	311, 365, 588, 758, 2136
HpyCH4III	ACNGT	7 cut(s)	7, 349, 774, 1055, 1706, 1846, 2185
HpyCH4IV	ACGT	1 cut(s)	477
HpyF10VI	GCNNNNNNNGC	8 cut(s)	524, 776, 1229, 1238, 1272, 1561, 1631, 1817
HpyF3I	CTNAG	5 cut(s)	639, 844, 916, 2087, 2163
HpySE526I	ACGT	1 cut(s)	477
Hsp92I	GRCGYC	2 cut(s)	138, 683
HspAI	GCGC	1 cut(s)	138
KasI	GGCGCC	1 cut(s)	137
KpnI	GGTACC	1 cut(s)	1770
Kzo9I	GATC	4 cut(s)	1354, 1540, 1571, 2099
LguI	GCTCTTC	1 cut(s)	1940
LmnI	GCTCC	6 cut(s)	107, 767, 961, 1987, 2026, 2150
Lsp1109I	GCAGC	4 cut(s)	889, 934, 1465, 1472
LweI	GCATC	7 cut(s)	604, 889, 1078, 1253, 1744, 1755, 1766
MabI	ACCWGGT	2 cut(s)	39, 1594
MaeI	CTAG	1 cut(s)	2279
MaeII	ACGT	1 cut(s)	477
MaeIII	GTNAC	4 cut(s)	7, 631, 1906, 1960
MalI	GATC	4 cut(s)	1356, 1542, 1573, 2101
MbiI	CCGCTC	1 cut(s)	403
MboI	GATC	4 cut(s)	1354, 1540, 1571, 2099
MboII	GAAGA	9 cut(s)	163, 577, 1110, 1374, 1750, 1927, 2058, 2094, 2151
MflI	RGATCY	3 cut(s)	1354, 1571, 2099
MhlI	GDGCHC	6 cut(s)	93, 608, 772, 1588, 1682, 1935
MluCI	AATT	6 cut(s)	284, 723, 812, 1087, 1589, 1815
Mly113I	GGCGCC	1 cut(s)	138
MlyI	GAGTC	1 cut(s)	1162
Mph1103I	ATGCAT	2 cut(s)	1268, 1759
MroXI	GAANNNNTTC	1 cut(s)	1082
MseI	TTAA	5 cut(s)	222, 660, 1140, 1914, 2296
MslI	CAYNNNNRTG	2 cut(s)	293, 885
MspA1I	CMGCKG	5 cut(s)	199, 274, 638, 947, 1463
MspI	CCGG	4 cut(s)	69, 77, 105, 217
MspR9I	CCNGG	8 cut(s)	41, 99, 515, 1002, 1220, 1524, 1596, 1764
MvaI	CCWGG	8 cut(s)	41, 99, 515, 1002, 1220, 1524, 1596, 1764
MvnI	CGCG	1 cut(s)	691
MwoI	GCNNNNNNNGC	8 cut(s)	524, 776, 1229, 1238, 1272, 1561, 1631, 1817
NarI	GGCGCC	1 cut(s)	138
NdeII	GATC	4 cut(s)	1354, 1540, 1571, 2099
NlaIV	GGNNCC	5 cut(s)	103, 139, 1356, 1645, 1768
NmuCI	GTSAC	3 cut(s)	7, 631, 1906
NsiI	ATGCAT	2 cut(s)	1268, 1759
NspI	RCATGY	8 cut(s)	485, 544, 860, 914, 1279, 1611, 2005, 2033
PaeI	GCATGC	2 cut(s)	485, 914
PaeR7I	CTCGAG	1 cut(s)	425
PceI	AGGCCT	2 cut(s)	849, 1992
PciI	ACATGT	3 cut(s)	856, 1607, 2001
PciSI	GCTCTTC	1 cut(s)	1940
PdmI	GAANNNNTTC	1 cut(s)	1082
PfeI	GAWTC	3 cut(s)	329, 437, 1372
PkrI	GCNGC	4 cut(s)	879, 949, 1462, 1480
PleI	GAGTC	1 cut(s)	1162
PluTI	GGCGCC	1 cut(s)	141
PpsI	GAGTC	1 cut(s)	1162
Ppu21I	YACGTR	1 cut(s)	478
PscI	ACATGT	3 cut(s)	856, 1607, 2001
PsiI	TTATAA	1 cut(s)	1395
Psp124BI	GAGCTC	1 cut(s)	1935
Psp6I	CCWGG	8 cut(s)	39, 97, 513, 1000, 1218, 1522, 1594, 1762
PspFI	CCCAGC	1 cut(s)	1630
PspGI	CCWGG	8 cut(s)	39, 97, 513, 1000, 1218, 1522, 1594, 1762
PspN4I	GGNNCC	5 cut(s)	103, 139, 1356, 1645, 1768
PspPI	GGNCC	2 cut(s)	370, 1558
PsuI	RGATCY	3 cut(s)	1354, 1571, 2099
PvuII	CAGCTG	2 cut(s)	638, 947
RsaI	GTAC	5 cut(s)	759, 926, 1152, 1768, 2182
RsaNI	GTAC	5 cut(s)	758, 925, 1151, 1767, 2181
RseI	CAYNNNNRTG	2 cut(s)	293, 885
SacI	GAGCTC	1 cut(s)	1935
SapI	GCTCTTC	1 cut(s)	1940
SaqAI	TTAA	5 cut(s)	222, 660, 1140, 1914, 2296
SatI	GCNGC	4 cut(s)	878, 948, 1461, 1479
Sau3AI	GATC	4 cut(s)	1354, 1540, 1571, 2099
Sau96I	GGNCC	2 cut(s)	370, 1558
SchI	GAGTC	1 cut(s)	1162
ScrFI	CCNGG	8 cut(s)	41, 99, 515, 1002, 1220, 1524, 1596, 1764
SduI	GDGCHC	6 cut(s)	93, 608, 772, 1588, 1682, 1935
SexAI	ACCWGGT	2 cut(s)	39, 1594
SfaNI	GCATC	7 cut(s)	604, 889, 1078, 1253, 1744, 1755, 1766
SfcI	CTRYAG	1 cut(s)	528
SfoI	GGCGCC	1 cut(s)	139
Sfr274I	CTCGAG	1 cut(s)	425
SinI	GGWCC	1 cut(s)	1558
SlaI	CTCGAG	1 cut(s)	425
SmiMI	CAYNNNNRTG	2 cut(s)	293, 885
SmlI	CTYRAG	4 cut(s)	425, 1226, 1655, 1952
SmoI	CTYRAG	4 cut(s)	425, 1226, 1655, 1952
SphI	GCATGC	2 cut(s)	485, 914
Sse9I	AATT	6 cut(s)	284, 723, 812, 1087, 1589, 1815
SseBI	AGGCCT	2 cut(s)	849, 1992
SsiI	CCGC	6 cut(s)	199, 272, 403, 689, 1463, 1744
SspDI	GGCGCC	1 cut(s)	137
SspMI	CTAG	1 cut(s)	2279
SstI	GAGCTC	1 cut(s)	1935
StuI	AGGCCT	2 cut(s)	849, 1992
StyD4I	CCNGG	8 cut(s)	39, 97, 513, 1000, 1218, 1522, 1594, 1762
StyI	CCWWGG	2 cut(s)	738, 1408
TaaI	ACNGT	7 cut(s)	7, 349, 774, 1055, 1706, 1846, 2185
TaiI	ACGT	1 cut(s)	480
TaqI	TCGA	3 cut(s)	426, 588, 1539
TasI	AATT	6 cut(s)	284, 723, 812, 1087, 1589, 1815
TatI	WGTACW	2 cut(s)	757, 1150
TfiI	GAWTC	3 cut(s)	329, 437, 1372
Tru1I	TTAA	5 cut(s)	222, 660, 1140, 1914, 2296
Tru9I	TTAA	5 cut(s)	222, 660, 1140, 1914, 2296
TscAI	CASTG	8 cut(s)	979, 1294, 1519, 1780, 1905, 1911, 2020, 2095
TseFI	GTSAC	3 cut(s)	7, 631, 1906
TseI	GCWGC	4 cut(s)	877, 947, 1460, 1478
Tsp45I	GTSAC	3 cut(s)	7, 631, 1906
TspGWI	ACGGA	5 cut(s)	66, 368, 1275, 1571, 2216
TspRI	CASTG	8 cut(s)	979, 1294, 1519, 1780, 1905, 1911, 2020, 2095
VneI	GTGCAC	1 cut(s)	604
VpaK11BI	GGWCC	1 cut(s)	1558
XapI	RAATTY	1 cut(s)	812
XceI	RCATGY	8 cut(s)	485, 544, 860, 914, 1279, 1611, 2005, 2033
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2020
XhoI	CTCGAG	1 cut(s)	425
XmnI	GAANNNNTTC	1 cut(s)	1082
XspI	CTAG	1 cut(s)	2279
Zsp2I	ATGCAT	2 cut(s)	1268, 1759

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.