MD10G1341500.v1.1

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000881
Physical Location & Seq
Forward (+)
41645649 .. 41647746
2098 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1341500.v1.1.491

Sequence Viewer

Length: 978 bp
ATGAAATGTGGGACTCCTCCCCCCTTTCAAATCAGACTCTGGGAACCCAACAGAGGGCAATATCCTCACAAACACAACAACACTCTCTTTCTCTGGGGGACTCCTCCTCATCCTTGTAATCCATACATACAGTCAGAGAAATACAATCAATGTGGACGTAGCCCAAACATTAGGGTGAACCACGATACATCTTGTGTTATTTACATTTCTTGCAGATTCACGGTCGGATTTACGTTGTTCCAAGACCTCCGGTTTTGTGCCGGTTTTGGTGGTACTCCAGCGAAGTTTTTGATAATTGACGATGGATGGCAAGATACATCAAATGAGTTCCAAATAGAAGGGGAACCATTTGTTGACGGATCACAGTTTGGTGGTAGATTAAATAGCATTCAAGAAAATAACAAGTTTCGTAGGACAACTAATAAAGAGGGTGAAAGTGAGACGCCAAGTAGTCTCAAGGATTTTGTATCTGAAATCAAGGGGACTTTTGGTCTTAAGTACGTCTATGTGTGGCATGCCCTGTTGGGATACTGGGGAGGGCTTCTTCCAAATGCTCTTGGAACTAAAAAGTACAATCCTGAGCTAAGATACCCAGTGCAGTCACCTGGGAATTTGGCAAATATGAGGGACTTAGCTATGGATTGCATGGAGAAATATGGTGTTGGTACAATAGATCCTGCAAAGGTGTATCAGTTTTATGATGATCTACACGGTTATCTTGTTTCACAAAACGTTGATGGGGTTAAGGTTGATGTTCAGAACATACTGGAGACGATATCAGCTGATTTAGGAGGGCGAGTTTCTCTGACCAGACAGTTCCAACAGGCACTGGAGAAGTCCATAGCCACCCACTTCCAAGACAACAGCATCATCTGCTGCATGGGCCAAAACACGGACTCCATTTACCAGTATATACTTATGCTTTACAATGCCCCCATCAGATTTTGTTTGGAAAAACTTAGGACCTCCAGTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

326

Amino Acids

36.9

Weight (kDa)

6.65

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 90 - 303 2.1e-70 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 732
AclWI GGATC 2 cut(s) 367, 668
AcsI RAATTY 1 cut(s) 610
AcyI GRCGYC 1 cut(s) 443
AfaI GTAC 4 cut(s) 274, 500, 572, 667
AfiI CCNNNNNNNGG 3 cut(s) 53, 54, 892
AflII CTTAAG 1 cut(s) 494
AgsI TTSAA 2 cut(s) 29, 392
AhdI GACNNNNNGTC 1 cut(s) 489
AjnI CCWGG 1 cut(s) 604
AluBI AGCT 3 cut(s) 583, 635, 782
AluI AGCT 3 cut(s) 583, 635, 782
Alw26I GTCTC 3 cut(s) 434, 458, 764
AlwI GGATC 2 cut(s) 367, 668
AlwNI CAGNNNCTG 2 cut(s) 39, 829
AoxI GGCC 1 cut(s) 883
ApeKI GCWGC 1 cut(s) 876
ApoI RAATTY 1 cut(s) 610
AspS9I GGNCC 2 cut(s) 883, 963
AsuHPI GGTGA 3 cut(s) 187, 443, 594
AvaII GGWCC 1 cut(s) 963
BbvI GCAGC 1 cut(s) 863
BccI CCATC 4 cut(s) 296, 300, 731, 944
BciT130I CCWGG 1 cut(s) 606
BciVI GTATCC 1 cut(s) 521
BcoDI GTCTC 3 cut(s) 434, 458, 764
BfrI CTTAAG 1 cut(s) 494
BfuI GTATCC 1 cut(s) 521
BisI GCNGC 1 cut(s) 877
BlsI GCNGC 1 cut(s) 878
Bme1390I CCNGG 1 cut(s) 606
Bme18I GGWCC 1 cut(s) 963
BmeRI GACNNNNNGTC 1 cut(s) 489
BmgT120I GGNCC 2 cut(s) 883, 963
BmiI GGNNCC 2 cut(s) 45, 345
BmrFI CCNGG 1 cut(s) 606
BmrI ACTGGG 2 cut(s) 541, 587
BmsI GCATC 1 cut(s) 876
BmuI ACTGGG 2 cut(s) 541, 587
BpmI CTGGAG 4 cut(s) 261, 788, 851, 952
Bpu10I CCTNAGC 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 440
BsaHI GRCGYC 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 605
BsaWI WCCGGW 1 cut(s) 249
BsaXI ACNNNNNCTCC 4 cut(s) 783, 813, 881, 911
Bsc4I CCNNNNNNNGG 3 cut(s) 53, 54, 892
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 6 cut(s) 536, 593, 771, 834, 907, 969
BseBI CCWGG 1 cut(s) 606
BseDI CCNNGG 1 cut(s) 605
BseGI GGATG 2 cut(s) 109, 311
BseLI CCNNNNNNNGG 3 cut(s) 53, 54, 892
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 6 cut(s) 536, 593, 771, 834, 907, 969
BseRI GAGGAG 3 cut(s) 6, 93, 96
BseXI GCAGC 1 cut(s) 863
BsgI GTGCAG 1 cut(s) 617
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 1 cut(s) 885
BsiEI CGRYCG 1 cut(s) 225
BsiSI CCGG 2 cut(s) 250, 261
BslFI GGGAC 4 cut(s) 25, 112, 496, 641
BslI CCNNNNNNNGG 3 cut(s) 53, 54, 892
BsmAI GTCTC 3 cut(s) 434, 458, 764
BsmBI CGTCTC 2 cut(s) 434, 764
BsmFI GGGAC 4 cut(s) 25, 112, 496, 641
BsmI GAATGC 1 cut(s) 387
BsnI GGCC 1 cut(s) 885
Bsp143I GATC 3 cut(s) 359, 673, 703
BspANI GGCC 1 cut(s) 885
BspCNI CTCAG 1 cut(s) 571
BspLI GGNNCC 2 cut(s) 45, 345
BspPI GGATC 2 cut(s) 367, 668
BspTI CTTAAG 1 cut(s) 494
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 6 cut(s) 536, 593, 771, 834, 907, 969
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 605
BssMI GATC 3 cut(s) 359, 673, 703
BssNI GRCGYC 1 cut(s) 443
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 5 cut(s) 132, 223, 366, 713, 816
BstACI GRCGYC 1 cut(s) 443
BstAFI CTTAAG 1 cut(s) 494
BstAPI GCANNNNNTGC 1 cut(s) 873
BstC8I GCNNGC 1 cut(s) 516
BstDEI CTNAG 4 cut(s) 579, 584, 631, 959
BstF5I GGATG 2 cut(s) 109, 311
BstKTI GATC 3 cut(s) 362, 676, 706
BstMAI GTCTC 3 cut(s) 434, 458, 764
BstMBI GATC 3 cut(s) 359, 673, 703
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 2 cut(s) 873, 882
BstNI CCWGG 1 cut(s) 606
BstNSI RCATGY 1 cut(s) 518
BstSCI CCNGG 1 cut(s) 604
BstV1I GCAGC 1 cut(s) 863
BstX2I RGATCY 1 cut(s) 673
BstYI RGATCY 1 cut(s) 673
BsuI GTATCC 1 cut(s) 521
BsuRI GGCC 1 cut(s) 885
BtsCI GGATG 2 cut(s) 109, 311
BtsIMutI CAGTG 2 cut(s) 600, 827
Cac8I GCNNGC 1 cut(s) 516
CaiI CAGNNNCTG 2 cut(s) 39, 829
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 2 cut(s) 883, 963
CseI GACGC 1 cut(s) 451
Csp6I GTAC 4 cut(s) 273, 499, 571, 666
CspCI CAANNNNNGTGG 2 cut(s) 133, 168
CviAII CATG 3 cut(s) 515, 646, 880
CviJI RGCY 7 cut(s) 162, 541, 583, 635, 782, 845, 885
CviKI_1 RGCY 7 cut(s) 162, 541, 583, 635, 782, 845, 885
CviQI GTAC 4 cut(s) 273, 499, 571, 666
DdeI CTNAG 4 cut(s) 579, 584, 631, 959
DpnI GATC 3 cut(s) 361, 675, 705
DpnII GATC 3 cut(s) 359, 673, 703
DriI GACNNNNNGTC 1 cut(s) 489
Eam1105I GACNNNNNGTC 1 cut(s) 489
Eco32I GATATC 1 cut(s) 777
Eco47I GGWCC 1 cut(s) 963
EcoO109I RGGNCCY 1 cut(s) 963
EcoRII CCWGG 1 cut(s) 604
EcoRV GATATC 1 cut(s) 777
Esp3I CGTCTC 2 cut(s) 434, 764
FaeI CATG 3 cut(s) 518, 649, 883
FaqI GGGAC 4 cut(s) 25, 112, 496, 641
FatI CATG 3 cut(s) 514, 645, 879
Fnu4HI GCNGC 1 cut(s) 877
FokI GGATG 2 cut(s) 96, 318
Fsp4HI GCNGC 1 cut(s) 877
GluI GCNGC 1 cut(s) 877
GsuI CTGGAG 4 cut(s) 261, 788, 851, 952
HaeIII GGCC 1 cut(s) 885
HapII CCGG 2 cut(s) 250, 261
HgaI GACGC 1 cut(s) 451
Hin1I GRCGYC 1 cut(s) 443
Hin1II CATG 3 cut(s) 518, 649, 883
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 5 cut(s) 13, 36, 100, 216, 896
HpaII CCGG 2 cut(s) 250, 261
HphI GGTGA 3 cut(s) 187, 443, 594
Hpy166II GTNNAC 3 cut(s) 155, 178, 355
Hpy188I TCNGA 7 cut(s) 35, 136, 227, 472, 759, 807, 941
Hpy188III TCNNGA 2 cut(s) 392, 578
Hpy8I GTNNAC 3 cut(s) 155, 178, 355
HpyAV CCTTC 1 cut(s) 332
HpyCH4III ACNGT 5 cut(s) 132, 223, 366, 713, 816
HpyCH4IV ACGT 4 cut(s) 157, 233, 501, 732
HpyCH4V TGCA 5 cut(s) 213, 598, 645, 680, 879
HpyF10VI GCNNNNNNNGC 2 cut(s) 873, 882
HpyF3I CTNAG 4 cut(s) 579, 584, 631, 959
HpySE526I ACGT 4 cut(s) 157, 233, 501, 732
Hsp92I GRCGYC 1 cut(s) 443
Hsp92II CATG 3 cut(s) 518, 649, 883
Kzo9I GATC 3 cut(s) 359, 673, 703
Lsp1109I GCAGC 1 cut(s) 863
LweI GCATC 1 cut(s) 876
MaeII ACGT 4 cut(s) 157, 233, 501, 732
MaeIII GTNAC 1 cut(s) 600
MalI GATC 3 cut(s) 361, 675, 705
MboI GATC 3 cut(s) 359, 673, 703
MboII GAAGA 1 cut(s) 536
MflI RGATCY 1 cut(s) 673
MluCI AATT 2 cut(s) 294, 610
MlyI GAGTC 4 cut(s) 7, 30, 94, 890
MmeI TCCRAC 2 cut(s) 205, 844
MseI TTAA 3 cut(s) 380, 495, 744
MslI CAYNNNNRTG 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 782
MspCI CTTAAG 1 cut(s) 494
MspI CCGG 2 cut(s) 250, 261
MspR9I CCNGG 1 cut(s) 606
Mva1269I GAATGC 1 cut(s) 387
MvaI CCWGG 1 cut(s) 606
MwoI GCNNNNNNNGC 2 cut(s) 873, 882
NdeII GATC 3 cut(s) 359, 673, 703
NlaIII CATG 3 cut(s) 518, 649, 883
NlaIV GGNNCC 2 cut(s) 45, 345
NmuCI GTSAC 1 cut(s) 600
NspI RCATGY 1 cut(s) 518
PaeI GCATGC 1 cut(s) 518
PctI GAATGC 1 cut(s) 387
PfeI GAWTC 1 cut(s) 216
PkrI GCNGC 1 cut(s) 878
PleI GAGTC 4 cut(s) 7, 30, 94, 890
PpsI GAGTC 4 cut(s) 7, 30, 94, 890
PpuMI RGGWCCY 1 cut(s) 963
Psp1406I AACGTT 1 cut(s) 732
Psp5II RGGWCCY 1 cut(s) 963
Psp6I CCWGG 1 cut(s) 604
PspGI CCWGG 1 cut(s) 604
PspN4I GGNNCC 2 cut(s) 45, 345
PspPI GGNCC 2 cut(s) 883, 963
PspPPI RGGWCCY 1 cut(s) 963
PstNI CAGNNNCTG 2 cut(s) 39, 829
PsuI RGATCY 1 cut(s) 673
PvuII CAGCTG 1 cut(s) 782
RsaI GTAC 4 cut(s) 274, 500, 572, 667
RsaNI GTAC 4 cut(s) 273, 499, 571, 666
RseI CAYNNNNRTG 1 cut(s) 173
SaqAI TTAA 3 cut(s) 380, 495, 744
SatI GCNGC 1 cut(s) 877
Sau3AI GATC 3 cut(s) 359, 673, 703
Sau96I GGNCC 2 cut(s) 883, 963
SchI GAGTC 4 cut(s) 7, 30, 94, 890
ScrFI CCNGG 1 cut(s) 606
SfaNI GCATC 1 cut(s) 876
SinI GGWCC 1 cut(s) 963
SmiMI CAYNNNNRTG 1 cut(s) 173
SmlI CTYRAG 2 cut(s) 455, 494
SmoI CTYRAG 2 cut(s) 455, 494
SphI GCATGC 1 cut(s) 518
Sse9I AATT 2 cut(s) 294, 610
StyD4I CCNGG 1 cut(s) 604
TaaI ACNGT 5 cut(s) 132, 223, 366, 713, 816
TaiI ACGT 4 cut(s) 160, 236, 504, 735
TasI AATT 2 cut(s) 294, 610
TatI WGTACW 1 cut(s) 570
TfiI GAWTC 1 cut(s) 216
Tru1I TTAA 3 cut(s) 380, 495, 744
Tru9I TTAA 3 cut(s) 380, 495, 744
TscAI CASTG 2 cut(s) 600, 834
TseFI GTSAC 1 cut(s) 600
TseI GCWGC 1 cut(s) 876
Tsp45I GTSAC 1 cut(s) 600
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 372, 908
TspRI CASTG 2 cut(s) 600, 834
Vha464I CTTAAG 1 cut(s) 494
VpaK11BI GGWCC 1 cut(s) 963
XapI RAATTY 1 cut(s) 610
XceI RCATGY 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.