Prupe.4G001700_v2.0.a1

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000881
Physical Location & Seq
Reverse (-)
150240 .. 156329
6090 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G001700.1

Sequence Viewer

Length: 2382 bp
ATGCTTCAGACCACCGTGTTTTCTCCATCTCCCGGAGCCCTTCAGCTCAACCACAACCACAACCGCAGGATCTTCTCGTGGGGACGAGGGTCTGTTGGTGTTAACAGCAGCACTAGTAACACATGGAGGCAATCTTTGTTTGTCAGCGCAAAACCTGTCCTTGAAGATGGTGTTCTCAGTGTCAATGGTAAGGAAGTGTTGACAAAAGTGCCTGAGAATGTGGTTGTCACACCATTGACCAACTCATCCGCATTTGTGGGTGCCACTTCTGAAACTGCTACTTCACGCCATGTTTTTAAGCTTGGAGTCATACGTGATGTCAGATTATTAAGTCTTTTTAGATTTAAGCTTTGGTGGATGATACCACGAGTGGGCAGTACGGGAAGTGACATTCCGGTTGAAACTCAGATGTTGCTCCTTCAAGCAAAGGAGGGACCAGATTTTGATGCGTTAAAGGAAGCTGCTCCTTATATCTTATTCTTGCCTGTTCTAGATGGTGAATTTAGAAGCAGCTTGCAGGGGAATTCATCGAATGAGCTTGAGTTCTGTGTTGAAAGTGGTGACCCAGCTATAGTTACCTCACAATCCCCAAAAGCAGTTTTTGTGAACTGTGGGAACCATCCTTTCGATCTGTTGAAGGAATCTATGAAGATTTTGGAGAAGCATTTTGGAACCTTTTCTCTTAGAGAATCCAAACAGATGCCTGGAATGCTAGATTGGTTTGGCTGGTGTACCTGGGATGCCTTTTATCAAGGAGTTAATCCTCAGGGAATCAGAGAAGGCCTGGAAAGTTTATCACAGGGAGGTACTCCAGCTAAGTTTTTGATAATTGACGATGGGTGGCAAGATACGTCAAATGAGTTCCAAATAGAAGGGGAGCCATTTGTTGAAGGATCACAGTTTGGTGGTAGATTAAATAGCATTCAAGAAAATAATAAGTTTCGTACAACAACCAATAAAGAGGCTGAAAGCGAGACACCAAGTGGTCTGAAGGAATTTGTTTCTGAAATCAAGGCGAATTTTGGCCTCAAGTACGTCTATGTATGGCATGCCCTGTTGGGATACTGGGGAGGGCTTCTTCCAAATGCTCTTGGAACTAAAAAGTATAATCCTAAGCTAAGATACCCAGTACAGTCCCCCGGGAATTTGGCAAATATGAGGGACTTGGCCATGGATTGCATGGAGAAATATGGTGTTGGTGCAATAGATCCTGCAAAGGTGTATCAGTTTTATGATGATCTACACGGTTATCTTGTTTCACAGAATGTGGATGGGGTTAAGGTCGATGTTCAGAACATACTGGAGACTATATCAACTGGTTTAGGAGGGCGCGTCTCTCTAACTAGACAGTTCCAACAGGCACTCGAGAAGTCCATAGCCACCCACTTCCAAGACAACAGCATAATCTGCTGCATGGGCCAAAGCACTGACTCCATTTACCATTCGAAAAAAAGTGCTATTACTCGAGCATCTGATGATTACTACCCAGAAAACCCAACAACACAGACACTACACGTTGCTGCTGTGGCTTTCAACAGCATATTCCTTGGTGAAGTTGTTGTCCCAGATTGGGACATGTTCTATAGCCGTCATGATGCAGCTGAGTTCCATGCTGCTGCTAGAGCTGTGGGAGGCTGTGGAGTCTATGTTAGTGACAAACCTGGCCAGCATGATTTCGAGATACTTAAAAGGCTTGTACTTCCCGATGGGTCAATTCTCAGAGCTCGATACCCAGGGAGGCCATCGCGGGATTGTTTATTTGTTGACCCAGTTATGGATGGGAAGAGCCTTCTAAAGATATGGAATTTGAACAAATGCAATGGAGTTATAGGCATTTTCAACTGCCAGGGAGCAGGGAAGTGGCCTTGTGTGGAAAACATTGTTGAGGTAAAAGCTAGTGCTGCTGAGCTATCCGGGCAGGTGTCCCCTGCAGATATTGAGTATTTCGAAGAGGTTTCTGGGAAGCATTGGACTGGAGATTGTGCAGTCTATTCCTTCACCAAAGGGTGCCTGTCTCGTTTACCAAAGGACAAGTCGTTTGAGGTCACATTAAAACTTCTCCAGTGTGATGTCTTTACTGTATCTCCTATTAAGGTTTACAAGCAAGAGATTGAGTTTGCAGCTATTGGATTATTGAATATGTACAATTCCGGTGGAGCAGTTGAAGCAATTGACTGCTTTGGTGATGAGTCTAGTTGTGAAATACACATCAAGGGAAGAGGAGGAGCTGGTAGTTTTGGAGCATACTCCAGTTTAAAGCCCAAAGCTTGCTCAGTCAACTCCATAGACGAGGAGGAATTTGAATTCAGAGGTGAAGACAATCTTTTAACAGTAACACTTCCCCCCAGAACAAGTTGTTGGAACATTATTCTAAGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

794

Amino Acids

87.53

Weight (kDa)

5.49

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1909
Acc36I ACCTGC 1 cut(s) 1909
AccB1I GGYRCC 2 cut(s) 260, 2007
AccII CGCG 2 cut(s) 1332, 1747
AciI CCGC 3 cut(s) 64, 249, 1747
AclWI GGATC 3 cut(s) 77, 901, 1202
AcoI YGGCCR 2 cut(s) 1167, 1663
AcsI RAATTY 8 cut(s) 500, 523, 995, 1018, 1144, 1804, 2297, 2303
AcuI CTGAAG 2 cut(s) 26, 1010
AdeI CACNNNGTG 1 cut(s) 983
AfaI GTAC 8 cut(s) 379, 733, 808, 946, 1034, 1131, 1698, 2144
AfiI CCNNNNNNNGG 4 cut(s) 32, 371, 1570, 1774
AflIII ACRYGT 2 cut(s) 1513, 1575
AhlI ACTAGT 1 cut(s) 113
AjnI CCWGG 6 cut(s) 703, 734, 783, 1660, 1732, 1845
AjuI GAANNNNNNNTTGG 4 cut(s) 700, 732, 2341, 2373
Alw21I GWGCWC 1 cut(s) 1726
Alw26I GTCTC 4 cut(s) 968, 1298, 1339, 2019
AlwI GGATC 3 cut(s) 77, 901, 1202
Ama87I CYCGRG 3 cut(s) 1139, 1364, 1464
AoxI GGCC 7 cut(s) 781, 1024, 1167, 1417, 1663, 1739, 1862
ApoI RAATTY 8 cut(s) 500, 523, 995, 1018, 1144, 1804, 2297, 2303
ArsI GACNNNNNNTTYG 4 cut(s) 2018, 2021, 2050, 2053
Asp700I GAANNNNTTC 1 cut(s) 676
AspLEI GCGC 2 cut(s) 149, 1332
AspS9I GGNCC 2 cut(s) 434, 1417
AsuC2I CCSGG 4 cut(s) 33, 1140, 1141, 1915
AsuHPI GGTGA 6 cut(s) 509, 572, 1562, 1990, 2195, 2324
AsuII TTCGAA 2 cut(s) 1445, 1947
AvaI CYCGRG 3 cut(s) 1139, 1364, 1464
AvaII GGWCC 1 cut(s) 434
AxyI CCTNAGG 1 cut(s) 765
BalI TGGCCA 2 cut(s) 1169, 1665
BanI GGYRCC 2 cut(s) 260, 2007
BanII GRGCYC 2 cut(s) 40, 1726
BauI CACGAG 2 cut(s) 76, 366
BbsI GAAGAC 1 cut(s) 2322
Bbv12I GWGCWC 1 cut(s) 1726
BccI CCATC 9 cut(s) 34, 161, 488, 627, 830, 1265, 1700, 1750, 1772
BceAI ACGGC 1 cut(s) 1572
BciT130I CCWGG 6 cut(s) 705, 736, 785, 1662, 1734, 1847
BciVI GTATCC 1 cut(s) 1055
BcnI CCSGG 4 cut(s) 33, 1140, 1141, 1915
BcoDI GTCTC 4 cut(s) 968, 1298, 1339, 2019
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 7 cut(s) 114, 491, 713, 1344, 1620, 1896, 2193
BfmI CTRYAG 3 cut(s) 570, 1582, 1929
BfuAI ACCTGC 1 cut(s) 1909
BfuI GTATCC 1 cut(s) 1055
BlpI GCTNAGC 1 cut(s) 1905
Bme18I GGWCC 1 cut(s) 434
BmeT110I CYCGRG 3 cut(s) 1139, 1364, 1464
BmgT120I GGNCC 2 cut(s) 434, 1417
BmiI GGNNCC 7 cut(s) 37, 262, 435, 617, 673, 879, 2009
BmrI ACTGGG 3 cut(s) 1075, 1121, 1763
BmsI GCATC 5 cut(s) 436, 690, 730, 1478, 1585
BmuI ACTGGG 3 cut(s) 1075, 1121, 1763
BoxI GACNNNNGTC 1 cut(s) 88
BpiI GAAGAC 1 cut(s) 2322
BpmI CTGGAG 5 cut(s) 795, 1322, 1995, 2045, 2233
Bpu10I CCTNAGC 1 cut(s) 1113
Bpu1102I GCTNAGC 1 cut(s) 1905
Bpu14I TTCGAA 2 cut(s) 1445, 1947
BpuEI CTTGAG 2 cut(s) 560, 1013
BpuMI CCSGG 4 cut(s) 33, 1140, 1141, 1915
BsaAI YACGTR 1 cut(s) 314
BsaJI CCNNGG 8 cut(s) 735, 1138, 1139, 1170, 1546, 1732, 1733, 1846
BsaWI WCCGGW 2 cut(s) 394, 2150
BsaXI ACNNNNNCTCC 6 cut(s) 1317, 1347, 1632, 1662, 2068, 2098
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 371, 1570, 1774
Bse1I ACTGG 8 cut(s) 1070, 1127, 1305, 1321, 1769, 1978, 2062, 2250
Bse21I CCTNAGG 1 cut(s) 765
Bse3DI GCAATG 1 cut(s) 1825
BseBI CCWGG 6 cut(s) 705, 736, 785, 1662, 1734, 1847
BseDI CCNNGG 8 cut(s) 735, 1138, 1139, 1170, 1546, 1732, 1733, 1846
BseGI GGATG 6 cut(s) 245, 363, 619, 745, 1276, 1783
BseLI CCNNNNNNNGG 4 cut(s) 32, 371, 1570, 1774
BseMI GCAATG 1 cut(s) 1825
BseMII CTCAG 8 cut(s) 190, 204, 419, 779, 1593, 1732, 1896, 2286
BseNI ACTGG 8 cut(s) 1070, 1127, 1305, 1321, 1769, 1978, 2062, 2250
BseRI GAGGAG 3 cut(s) 2235, 2238, 2306
BseYI CCCAGC 1 cut(s) 565
BsgI GTGCAG 1 cut(s) 2004
Bsh1236I CGCG 2 cut(s) 1332, 1747
BshFI GGCC 7 cut(s) 783, 1026, 1169, 1419, 1665, 1741, 1864
BshNI GGYRCC 2 cut(s) 260, 2007
BsiHKAI GWGCWC 1 cut(s) 1726
BsiHKCI CYCGRG 3 cut(s) 1139, 1364, 1464
BsiSI CCGG 5 cut(s) 33, 395, 1140, 1914, 2151
BslFI GGGAC 7 cut(s) 96, 447, 1120, 1175, 1547, 1586, 1909
BslI CCNNNNNNNGG 4 cut(s) 32, 371, 1570, 1774
BsmAI GTCTC 4 cut(s) 968, 1298, 1339, 2019
BsmBI CGTCTC 1 cut(s) 1339
BsmFI GGGAC 7 cut(s) 96, 447, 1120, 1175, 1547, 1586, 1909
BsmI GAATGC 2 cut(s) 714, 921
BsnI GGCC 7 cut(s) 783, 1026, 1169, 1419, 1665, 1741, 1864
BsoBI CYCGRG 3 cut(s) 1139, 1364, 1464
Bsp119I TTCGAA 2 cut(s) 1445, 1947
Bsp1286I GDGCHC 2 cut(s) 40, 1726
Bsp1407I TGTACA 1 cut(s) 2142
Bsp143I GATC 5 cut(s) 69, 628, 893, 1207, 1237
Bsp1720I GCTNAGC 1 cut(s) 1905
Bsp19I CCATGG 1 cut(s) 1170
BspACI CCGC 3 cut(s) 64, 249, 1747
BspANI GGCC 7 cut(s) 783, 1026, 1169, 1419, 1665, 1741, 1864
BspCNI CTCAG 8 cut(s) 189, 205, 418, 778, 1594, 1731, 1897, 2285
BspFNI CGCG 2 cut(s) 1332, 1747
BspHI TCATGA 1 cut(s) 1591
BspLI GGNNCC 7 cut(s) 37, 262, 435, 617, 673, 879, 2009
BspMAI CTGCAG 1 cut(s) 1933
BspMI ACCTGC 1 cut(s) 1909
BspPI GGATC 3 cut(s) 77, 901, 1202
BspQI GCTCTTC 1 cut(s) 1778
BspT104I TTCGAA 2 cut(s) 1445, 1947
BspT107I GGYRCC 2 cut(s) 260, 2007
BsrDI GCAATG 1 cut(s) 1825
BsrGI TGTACA 1 cut(s) 2142
BsrI ACTGG 8 cut(s) 1070, 1127, 1305, 1321, 1769, 1978, 2062, 2250
BssECI CCNNGG 8 cut(s) 735, 1138, 1139, 1170, 1546, 1732, 1733, 1846
BssMI GATC 5 cut(s) 69, 628, 893, 1207, 1237
BssSI CACGAG 2 cut(s) 76, 366
BssT1I CCWWGG 2 cut(s) 1170, 1546
Bst2BI CACGAG 2 cut(s) 76, 366
Bst2UI CCWGG 6 cut(s) 705, 736, 785, 1662, 1734, 1847
Bst4CI ACNGT 8 cut(s) 16, 611, 900, 1134, 1247, 1350, 2080, 2332
Bst6I CTCTTC 3 cut(s) 1778, 1944, 2212
BstAPI GCANNNNNTGC 1 cut(s) 1407
BstAUI TGTACA 1 cut(s) 2142
BstBAI YACGTR 1 cut(s) 314
BstBI TTCGAA 2 cut(s) 1445, 1947
BstC8I GCNNGC 4 cut(s) 515, 1050, 1667, 2269
BstDSI CCRYGG 1 cut(s) 1170
BstEII GGTNACC 1 cut(s) 560
BstF5I GGATG 6 cut(s) 245, 363, 619, 745, 1276, 1783
BstFNI CGCG 2 cut(s) 1332, 1747
BstHHI GCGC 2 cut(s) 149, 1332
BstKTI GATC 5 cut(s) 72, 631, 896, 1210, 1240
BstMAI GTCTC 4 cut(s) 968, 1298, 1339, 2019
BstMBI GATC 5 cut(s) 69, 628, 893, 1207, 1237
BstMWI GCNNNNNNNGC 8 cut(s) 709, 1407, 1416, 1526, 1622, 1901, 1915, 2165
BstNI CCWGG 6 cut(s) 705, 736, 785, 1662, 1734, 1847
BstNSI RCATGY 2 cut(s) 1052, 1579
BstPAI GACNNNNGTC 1 cut(s) 88
BstPI GGTNACC 1 cut(s) 560
BstSFI CTRYAG 3 cut(s) 570, 1582, 1929
BstUI CGCG 2 cut(s) 1332, 1747
BstV2I GAAGAC 1 cut(s) 2322
BstX2I RGATCY 2 cut(s) 69, 1207
BstYI RGATCY 2 cut(s) 69, 1207
Bsu36I CCTNAGG 1 cut(s) 765
BsuI GTATCC 1 cut(s) 1055
BsuRI GGCC 7 cut(s) 783, 1026, 1169, 1419, 1665, 1741, 1864
BtgI CCRYGG 1 cut(s) 1170
BtgZI GCGATG 1 cut(s) 1728
BtsCI GGATG 6 cut(s) 245, 363, 619, 745, 1276, 1783
BtsIMutI CAGTG 3 cut(s) 184, 1425, 2069
BveI ACCTGC 1 cut(s) 1909
Cac8I GCNNGC 4 cut(s) 515, 1050, 1667, 2269
CciI TCATGA 1 cut(s) 1591
CfoI GCGC 2 cut(s) 149, 1332
Cfr13I GGNCC 2 cut(s) 434, 1417
Cfr9I CCCGGG 1 cut(s) 1139
CseI GACGC 1 cut(s) 1321
Csp6I GTAC 8 cut(s) 378, 732, 807, 945, 1033, 1130, 1697, 2143
CspCI CAANNNNNGTGG 2 cut(s) 2134, 2169
CviQI GTAC 8 cut(s) 378, 732, 807, 945, 1033, 1130, 1697, 2143
DpnI GATC 5 cut(s) 71, 630, 895, 1209, 1239
DpnII GATC 5 cut(s) 69, 628, 893, 1207, 1237
DraI TTTAAA 1 cut(s) 2256
DraIII CACNNNGTG 1 cut(s) 983
EaeI YGGCCR 2 cut(s) 1167, 1663
Eam1104I CTCTTC 3 cut(s) 1778, 1944, 2212
EarI CTCTTC 3 cut(s) 1778, 1944, 2212
Ecl136II GAGCTC 1 cut(s) 1724
Eco130I CCWWGG 2 cut(s) 1170, 1546
Eco147I AGGCCT 1 cut(s) 783
Eco24I GRGCYC 2 cut(s) 40, 1726
Eco47I GGWCC 1 cut(s) 434
Eco53kI GAGCTC 1 cut(s) 1724
Eco57I CTGAAG 2 cut(s) 26, 1010
Eco81I CCTNAGG 1 cut(s) 765
Eco88I CYCGRG 3 cut(s) 1139, 1364, 1464
Eco91I GGTNACC 1 cut(s) 560
EcoICRI GAGCTC 1 cut(s) 1724
EcoO65I GGTNACC 1 cut(s) 560
EcoRI GAATTC 2 cut(s) 523, 2303
EcoRII CCWGG 6 cut(s) 703, 734, 783, 1660, 1732, 1845
EcoT14I CCWWGG 2 cut(s) 1170, 1546
EcoT38I GRGCYC 2 cut(s) 40, 1726
ErhI CCWWGG 2 cut(s) 1170, 1546
Esp3I CGTCTC 1 cut(s) 1339
FalI AAGNNNNNCTT 2 cut(s) 2307, 2339
FaqI GGGAC 7 cut(s) 96, 447, 1120, 1175, 1547, 1586, 1909
FauI CCCGC 1 cut(s) 1740
FokI GGATG 6 cut(s) 232, 370, 606, 752, 1283, 1790
FriOI GRGCYC 2 cut(s) 40, 1726
FspBI CTAG 7 cut(s) 114, 491, 713, 1344, 1620, 1896, 2193
GlaI GCGC 2 cut(s) 148, 1331
GsaI CCCAGC 1 cut(s) 569
GsuI CTGGAG 5 cut(s) 795, 1322, 1995, 2045, 2233
HaeIII GGCC 7 cut(s) 783, 1026, 1169, 1419, 1665, 1741, 1864
HapII CCGG 5 cut(s) 33, 395, 1140, 1914, 2151
HgaI GACGC 1 cut(s) 1321
HhaI GCGC 2 cut(s) 149, 1332
Hin6I GCGC 2 cut(s) 147, 1330
HinP1I GCGC 2 cut(s) 147, 1330
HincII GTYRAC 4 cut(s) 103, 201, 1765, 2278
HindII GTYRAC 4 cut(s) 103, 201, 1765, 2278
HindIII AAGCTT 3 cut(s) 299, 347, 2265
HinfI GANTC 7 cut(s) 306, 641, 689, 771, 1430, 1641, 2189
HpaI GTTAAC 1 cut(s) 103
HpaII CCGG 5 cut(s) 33, 395, 1140, 1914, 2151
HphI GGTGA 6 cut(s) 509, 572, 1562, 1990, 2195, 2324
Hpy166II GTNNAC 8 cut(s) 103, 201, 607, 732, 1765, 2021, 2098, 2278
Hpy188III TCNNGA 6 cut(s) 491, 926, 1366, 1592, 1678, 1703
Hpy8I GTNNAC 8 cut(s) 103, 201, 607, 732, 1765, 2021, 2098, 2278
HpyAV CCTTC 9 cut(s) 50, 428, 631, 773, 866, 884, 985, 1799, 2005
HpyCH4III ACNGT 8 cut(s) 16, 611, 900, 1134, 1247, 1350, 2080, 2332
HpyCH4IV ACGT 4 cut(s) 313, 851, 1035, 1515
HpyF10VI GCNNNNNNNGC 8 cut(s) 709, 1407, 1416, 1526, 1622, 1901, 1915, 2165
HpySE526I ACGT 4 cut(s) 313, 851, 1035, 1515
HspAI GCGC 2 cut(s) 147, 1330
KspAI GTTAAC 1 cut(s) 103
Kzo9I GATC 5 cut(s) 69, 628, 893, 1207, 1237
LguI GCTCTTC 1 cut(s) 1778
LmnI GCTCC 8 cut(s) 35, 420, 469, 877, 1850, 2156, 2225, 2240
LweI GCATC 5 cut(s) 436, 690, 730, 1478, 1585
MaeI CTAG 7 cut(s) 114, 491, 713, 1344, 1620, 1896, 2193
MaeII ACGT 4 cut(s) 313, 851, 1035, 1515
MaeIII GTNAC 8 cut(s) 116, 226, 386, 560, 574, 1652, 2044, 2332
MalI GATC 5 cut(s) 71, 630, 895, 1209, 1239
MboI GATC 5 cut(s) 69, 628, 893, 1207, 1237
MboII GAAGA 8 cut(s) 64, 176, 661, 1070, 1795, 1961, 2229, 2327
MfeI CAATTG 1 cut(s) 2169
MflI RGATCY 2 cut(s) 69, 1207
MhlI GDGCHC 2 cut(s) 40, 1726
MlsI TGGCCA 2 cut(s) 1169, 1665
MluNI TGGCCA 2 cut(s) 1169, 1665
MlyI GAGTC 4 cut(s) 315, 1424, 1650, 2198
MmeI TCCRAC 2 cut(s) 1378, 2339
Mox20I TGGCCA 2 cut(s) 1169, 1665
MroXI GAANNNNTTC 1 cut(s) 676
MscI TGGCCA 2 cut(s) 1169, 1665
Msp20I TGGCCA 2 cut(s) 1169, 1665
MspA1I CMGCKG 1 cut(s) 1601
MspI CCGG 5 cut(s) 33, 395, 1140, 1914, 2151
MunI CAATTG 1 cut(s) 2169
Mva1269I GAATGC 2 cut(s) 714, 921
MvaI CCWGG 6 cut(s) 705, 736, 785, 1662, 1734, 1847
MvnI CGCG 2 cut(s) 1332, 1747
MwoI GCNNNNNNNGC 8 cut(s) 709, 1407, 1416, 1526, 1622, 1901, 1915, 2165
NciI CCSGG 4 cut(s) 33, 1140, 1141, 1915
NcoI CCATGG 1 cut(s) 1170
NdeII GATC 5 cut(s) 69, 628, 893, 1207, 1237
NlaIV GGNNCC 7 cut(s) 37, 262, 435, 617, 673, 879, 2009
NmuCI GTSAC 5 cut(s) 226, 386, 560, 1652, 2044
NspI RCATGY 2 cut(s) 1052, 1579
NspV TTCGAA 2 cut(s) 1445, 1947
PaeI GCATGC 1 cut(s) 1052
PaeR7I CTCGAG 2 cut(s) 1364, 1464
PagI TCATGA 1 cut(s) 1591
PaqCI CACCTGC 1 cut(s) 1909
PasI CCCWGGG 1 cut(s) 1733
PceI AGGCCT 1 cut(s) 783
PciI ACATGT 1 cut(s) 1575
PciSI GCTCTTC 1 cut(s) 1778
PctI GAATGC 2 cut(s) 714, 921
PdmI GAANNNNTTC 1 cut(s) 676
PfeI GAWTC 3 cut(s) 641, 689, 771
PfoI TCCNGGA 1 cut(s) 31
PleI GAGTC 4 cut(s) 314, 1424, 1649, 2197
PpsI GAGTC 4 cut(s) 314, 1424, 1649, 2197
Ppu21I YACGTR 1 cut(s) 314
PscI ACATGT 1 cut(s) 1575
PshAI GACNNNNGTC 1 cut(s) 88
Psp124BI GAGCTC 1 cut(s) 1726
Psp6I CCWGG 6 cut(s) 703, 734, 783, 1660, 1732, 1845
PspEI GGTNACC 1 cut(s) 560
PspFI CCCAGC 1 cut(s) 565
PspGI CCWGG 6 cut(s) 703, 734, 783, 1660, 1732, 1845
PspN4I GGNNCC 7 cut(s) 37, 262, 435, 617, 673, 879, 2009
PspPI GGNCC 2 cut(s) 434, 1417
PspXI VCTCGAGB 1 cut(s) 1464
PstI CTGCAG 1 cut(s) 1933
PsuI RGATCY 2 cut(s) 69, 1207
PvuII CAGCTG 1 cut(s) 1601
RsaI GTAC 8 cut(s) 379, 733, 808, 946, 1034, 1131, 1698, 2144
RsaNI GTAC 8 cut(s) 378, 732, 807, 945, 1033, 1130, 1697, 2143
SacI GAGCTC 1 cut(s) 1726
SapI GCTCTTC 1 cut(s) 1778
Sau3AI GATC 5 cut(s) 69, 628, 893, 1207, 1237
Sau96I GGNCC 2 cut(s) 434, 1417
SchI GAGTC 4 cut(s) 315, 1424, 1650, 2198
SduI GDGCHC 2 cut(s) 40, 1726
SfaNI GCATC 5 cut(s) 436, 690, 730, 1478, 1585
SfcI CTRYAG 3 cut(s) 570, 1582, 1929
Sfr274I CTCGAG 2 cut(s) 1364, 1464
SfuI TTCGAA 2 cut(s) 1445, 1947
SinI GGWCC 1 cut(s) 434
SlaI CTCGAG 2 cut(s) 1364, 1464
SmaI CCCGGG 1 cut(s) 1141
SmlI CTYRAG 4 cut(s) 539, 1028, 1364, 1464
SmoI CTYRAG 4 cut(s) 539, 1028, 1364, 1464
SpeI ACTAGT 1 cut(s) 113
SphI GCATGC 1 cut(s) 1052
SseBI AGGCCT 1 cut(s) 783
SsiI CCGC 3 cut(s) 64, 249, 1747
SspMI CTAG 7 cut(s) 114, 491, 713, 1344, 1620, 1896, 2193
SstI GAGCTC 1 cut(s) 1726
StuI AGGCCT 1 cut(s) 783
StyI CCWWGG 2 cut(s) 1170, 1546
TaaI ACNGT 8 cut(s) 16, 611, 900, 1134, 1247, 1350, 2080, 2332
TaiI ACGT 4 cut(s) 316, 854, 1038, 1518
TaqI TCGA 9 cut(s) 530, 627, 1284, 1365, 1445, 1465, 1677, 1726, 1947
TatI WGTACW 3 cut(s) 1129, 1696, 2142
TfiI GAWTC 3 cut(s) 641, 689, 771
TscAI CASTG 3 cut(s) 184, 1432, 2069
TseFI GTSAC 5 cut(s) 226, 386, 560, 1652, 2044
Tsp45I GTSAC 5 cut(s) 226, 386, 560, 1652, 2044
TspDTI ATGAA 2 cut(s) 516, 662
TspMI CCCGGG 1 cut(s) 1139
TspRI CASTG 3 cut(s) 184, 1432, 2069
VpaK11BI GGWCC 1 cut(s) 434
XapI RAATTY 8 cut(s) 500, 523, 995, 1018, 1144, 1804, 2297, 2303
XbaI TCTAGA 1 cut(s) 490
XceI RCATGY 2 cut(s) 1052, 1579
XcmI CCANNNNNNNNNTGG 1 cut(s) 1177
XhoI CTCGAG 2 cut(s) 1364, 1464
XmaI CCCGGG 1 cut(s) 1139
XmnI GAANNNNTTC 1 cut(s) 676
XspI CTAG 7 cut(s) 114, 491, 713, 1344, 1620, 1896, 2193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.