Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0000881

Physical Location & Seq

Genomic Coordinates Forward (+)

26449603 .. 26455099

Gene Structure 5497 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G021800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2304 bp

ATGACTGTCACTCCAAACATTTCCATCAACGATGGAAACCTGGTGGTTCACGGAAAGACCATATTGACCGGAGTGCCGGAAAACATTGTGCTCACTCCAGGATCCGGCGTCGGCCTCGTTGCTGGTGCTTTCATTGGCGCCACCGCCTCTCACAGCAAAAGCCTCCATGTCTTCCCTGTCGGTGTTTTAGAGGGTCAGCGGTTCATGTGCCTTTTCCGGTTTAAGCTGTGGTGGATGACGCAGAGGATGGGGTCATGTGGGAAAGAAGTTCCACTGGAGACCCAATTCATGCTGGTGGAGACCAAGGATGATGATGGTGAAGGAAGTGATGGGTGCTCTTCTACCATTTACACCGTCTTCCTTCCTCTGCTGGAGGGCCAGTTCCGTTCGGTTCTGCAAGGGAACGAGCGGAATGAAGTGGAGATATGCCTCGAGAGTGGAGATTCTGATGTGCAGACCAATCAAGGTCTTTGCCTTGTTTACGTGCATGCTGGAACTAATCCATTTGAAGTTATCAACCAGGCTGTGAAGGCTGTAGAAAAACACATGCAAACCTTTGTCCATCGTGAGAAGAAAAAGTTGCCTTCTTTCCTCGACTGGTTTGGCTGGTGCACATGGGATGCTTTCTATACCGATGTCACAGCTGAGGGTGTCGTGGATGGTCTTAAAAGCTTGAATGAGGGAGGGACGCCTCCGCGATTTCTAATCGTAGATGATGGTTGGCAACAAATTGAAAGTAAACCCAAGGATACTAATGTTGCAGTACAAGAAGGAGCACAGTTTGCAAGTAGGCTTACAGGAATAAAAGAGAATGAAAAATTCCAAAAGAATGGACAGAGTGAGCAAGTCTCAGGCCTGAAACATGTTGTTGATGAAGCGAAGCAGCATCACAATGTGAAGTTTGTGTACGTATGGCATGCCTTAGTTGGGTACTGGGGTGGAGTGAAACCAGCTGCTTCTGGCATGGAGCATTATGACACTGCTTTGGCTTATCCAGTCTCATCTCCTGGTGTGATGGGAAACCAACCTGACATAGTTTTTGACAGCTTATCTGTTCATGGTCTTGGTTTGGTGCATCCAAAGAAAGTTTTCAATTTCTACAATGAGCTTCATGCGTATTTGTCTTCCTGTGGAGTGGATGGAGTTAAGGTTGATGTACAGAACATCATTGAGACTCTTGGGTCTGGTCATGGTGGCAGAGTTTCTCTGACCCGAAGCTATCACCAGGCACTGGAGGCTTCGGTTGCTCGGAACTTTCCTGACAACGGATGCATTTCTTGCATGTGTCATAACACTGATGGGCTATATAGTTCCAAGCAGACTGCTGTTGTGAGAGCTTCTGATGACTTCTACCCACGGGATCCTGCTTCTCACACGATTCATATATCTTCTGTGGCTTATAACACTCTTTTCCTTGGCGAGTTTATGCAACCAGACTGGGATATGTTTCATAGTTTACATCCAGCAGCGGATTATCATGGTGCAGCTCGTTCTATTGGTGGATGTGCAATCTATGTCAGTGATAAACCTGGAAACCACAACTTCGATCTTTTGAGGAAGCTGGTCCTGCCTGATGGATCTGTCCTCCGTGCTCAATTACCTGGTAGGCCAACATGTGATTGTCTCTTTGCTGACCCAGCAAGAGATGGAACCAGCTTGCTCAAGATATGGAATGTGAACAAGTGCTCTGGTGTTGTTGGTGTATTCAACTGTCAAGGGGCCGGTTGGTGCAAGGTTGAAAAGAAGACCCGCATCCATGATGCATCTCCTGGTACCCTCACTGGTTCTGTTCGTGCTATTGATGTTGATAGCCTTGCCCAAATTGCTGGTGATGATTGGAATGGAGAAACAGTAGTTTATGCCCATAAGTCAGGAGAGGTGTTTCGTTTACCAAAAGATGTTTCAGTGCCAGTGACACTTAAAGTTCTGGAGTATGAGCTCTTCCATTTCTGTCCTCTCAAGGAGGTTACACCCAACATCTCATTTGCTCCAATAGGCCTGCTTGACATGTTCAACTCCAGTGGTGCTGTGGAGCATGTTGTAATCCATCTGGCTTCTGACGGCGAAGTTTCTTCAGAGCTTACCACTTCTCTCAGTGAGAATAGATCTTCAACTGCCACAGTCACCATCAAAACCAGAGGATGTGGAAGGTTTGGAGCTTACTCTTCTCAGCGTCCCCTGAAGTGTACCGTTGATAGAGTTGAGACGGACTTTGAATATGACACTGCAACTGGATTACTAACCTTTACCATCCCTATCCCAAAAGAGGATATGTATAAATGGCTAGTAGAAATCCAAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005911 GO:0005975 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009506 GO:0009628 GO:0015925 GO:0016052 GO:0016787 GO:0016798 GO:0030054 GO:0033530 GO:0034484 GO:0044238 GO:0050896 GO:0052692 GO:0055044 GO:0071704 GO:0080167 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00052 map00052

Pfam Domains

Protein Families

Raffinose_syn

Protein Analysis

767

Amino Acids

83.96

Weight (kDa)

5.68

Isoelectric Point (pI)

30.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Raffinose_syn	PF05691	7 - 750	0.0	Raffinose synthase or seed imbibition protein Sip1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0000881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57520 AT3G57520 AT3G57520
fragaria_vesca	FvH4_3g00160 FvH4_6g23550 FvH4_6g23550 FvH4_6g23550 FvH4_6g23550
malus_domestica	MD09G1161700.v1.1 MD09G1162100.v1.1 MD10G1341400.v1.1 MD10G1341500.v1.1 MD10G1341600.v1.1 MD12G1077800.v1.1 MD14G1070300.v1.1
prunus_persica	Prupe.4G001700_v2.0.a1 Prupe.5G162400_v2.0.a1 Prupe.5G162400_v2.0.a1 Prupe.7G064000_v2.0.a1 Prupe.7G064000_v2.0.a1
pyrus_communis	pycom10g29090 pycom12g06210 pycom14g05800 pycom14g05880 pycom14g05890 pycom14g05910 pycom14g05930
rosa_chinensis	RchiOBHm_Chr3g0481621 RchiOBHm_Chr5g0000211 RchiOBHm_Chr7g0192011 RchiOBHm_Chr7g0192021
rosa_laevigata	RLG00000004409 RLG00000023385 RLG00000030850
rosa_multiflora	Rmu_co8093432.1_g000001 Rmu_co8314355.1_g000001 Rmu_sc0010409.1_g000002 Rmu_sc0021936.1_g000002 Rmu_ssc0000243.1_g000010 Rmu_ssc0000421.1_g000033
rosa_roxburghii	Rroxscaffold_1G00075890 Rroxscaffold_3G00263360 Rroxscaffold_6G00400310
rosa_rugosa	Rorug03G0192700 Rorug03G0192800 Rorug03G0192900 Rorug04G0382500 Rorug06G0512300 Rorug06G0512300
rosa_samantha	Rh3AG244100 Rh3BG279100 Rh3BG279300 Rh3CG274100 Rh3DG270900 Rh5AG001400 Rh5BG002800 Rh5CG001700 Rh5DG001600 Rh7AG122700 Rh7AG122800 Rh7BG124300 Rh7CG127000 Rh7DG125800
rosa_wichuraiana	Rw3G021800 Rw5G000190 Rw7G010450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1401
AasI	GACNNNNNNGTC	1 cut(s)	1180
Acc65I	GGTACC	1 cut(s)	1772
AccB1I	GGYRCC	2 cut(s)	137, 1772
AccB7I	CCANNNNNTGG	1 cut(s)	1231
AccBSI	CCGCTC	1 cut(s)	409
AccII	CGCG	1 cut(s)	697
AciI	CCGC	6 cut(s)	144, 199, 409, 695, 1469, 1750
AclWI	GGATC	5 cut(s)	96, 109, 1355, 1368, 1585
AcsI	RAATTY	1 cut(s)	818
AcuI	CTGAAG	2 cut(s)	2058, 2201
AcyI	GRCGYC	3 cut(s)	108, 138, 689
AdeI	CACNNNGTG	1 cut(s)	895
AfaI	GTAC	6 cut(s)	765, 908, 932, 1158, 1774, 2188
AfiI	CCNNNNNNNGG	5 cut(s)	104, 927, 1231, 1265, 2266
AflIII	ACRYGT	3 cut(s)	862, 1613, 2007
AgsI	TTSAA	9 cut(s)	509, 676, 734, 1093, 1708, 1739, 2014, 2112, 2216
AjnI	CCWGG	8 cut(s)	39, 97, 519, 1006, 1224, 1528, 1600, 1768
AjuI	GAANNNNNNNTTGG	2 cut(s)	1885, 1917
AloI	GAACNNNNNNTCC	2 cut(s)	365, 397
Alw21I	GWGCWC	7 cut(s)	93, 338, 614, 778, 1594, 1688, 1941
Alw26I	GTCTC	7 cut(s)	272, 293, 853, 1003, 1166, 1628, 2198
Alw44I	GTGCAC	1 cut(s)	610
AlwI	GGATC	5 cut(s)	96, 109, 1355, 1368, 1585
AlwNI	CAGNNNCTG	1 cut(s)	1231
Ama87I	CYCGRG	1 cut(s)	431
AoxI	GGCC	6 cut(s)	112, 376, 853, 1607, 1719, 1996
ApaLI	GTGCAC	1 cut(s)	610
ApeKI	GCWGC	4 cut(s)	883, 953, 1466, 1484
ApoI	RAATTY	1 cut(s)	818
ArsI	GACNNNNNNTTYG	2 cut(s)	1022, 1054
Asp700I	GAANNNNTTC	1 cut(s)	1088
Asp718I	GGTACC	1 cut(s)	1772
AspLEI	GCGC	1 cut(s)	140
AspS9I	GGNCC	3 cut(s)	376, 1564, 1719
AsuHPI	GGTGA	4 cut(s)	329, 1214, 1841, 2116
AvaI	CYCGRG	1 cut(s)	431
AvaII	GGWCC	1 cut(s)	1564
BaeGI	GKGCMC	1 cut(s)	614
BaeI	ACNNNNGTAYC	4 cut(s)	741, 774, 922, 955
BamHI	GGATCC	2 cut(s)	101, 1360
BanI	GGYRCC	2 cut(s)	137, 1772
BanII	GRGCYC	1 cut(s)	1941
BarI	GAAGNNNNNNTAC	4 cut(s)	890, 922, 1925, 1957
BbsI	GAAGAC	4 cut(s)	163, 349, 1116, 1751
Bbv12I	GWGCWC	7 cut(s)	93, 338, 614, 778, 1594, 1688, 1941
BbvCI	CCTCAGC	1 cut(s)	645
BbvI	GCAGC	4 cut(s)	895, 940, 1478, 1496
BceAI	ACGGC	1 cut(s)	2077
BciT130I	CCWGG	8 cut(s)	41, 99, 521, 1008, 1226, 1530, 1602, 1770
BciVI	GTATCC	1 cut(s)	742
BcoDI	GTCTC	7 cut(s)	272, 293, 853, 1003, 1166, 1628, 2198
BfaI	CTAG	1 cut(s)	2285
BfmI	CTRYAG	1 cut(s)	534
BfoI	RGCGCY	1 cut(s)	141
BfuI	GTATCC	1 cut(s)	742
BglII	AGATCT	1 cut(s)	2105
BisI	GCNGC	4 cut(s)	884, 954, 1467, 1485
BlsI	GCNGC	4 cut(s)	885, 955, 1468, 1486
Bme1390I	CCNGG	8 cut(s)	41, 99, 521, 1008, 1226, 1530, 1602, 1770
Bme18I	GGWCC	1 cut(s)	1564
BmeT110I	CYCGRG	1 cut(s)	431
BmgT120I	GGNCC	3 cut(s)	376, 1564, 1719
BmiI	GGNNCC	6 cut(s)	103, 139, 1362, 1651, 1720, 1774
BmrFI	CCNGG	8 cut(s)	41, 99, 521, 1008, 1226, 1530, 1602, 1770
BmrI	ACTGGG	2 cut(s)	943, 1447
BmsI	GCATC	7 cut(s)	610, 895, 1084, 1259, 1750, 1761, 1772
BmuI	ACTGGG	2 cut(s)	943, 1447
BpiI	GAAGAC	4 cut(s)	163, 349, 1116, 1751
BplI	GAGNNNNNCTC	2 cut(s)	833, 865
BpmI	CTGGAG	6 cut(s)	81, 296, 392, 1253, 1949, 2002
Bpu10I	CCTNAGC	1 cut(s)	645
BpuEI	CTTGAG	2 cut(s)	1646, 1943
BsaAI	YACGTR	2 cut(s)	484, 910
BsaHI	GRCGYC	3 cut(s)	108, 138, 689
BsaI	GGTCTC	2 cut(s)	272, 293
BsaJI	CCNNGG	4 cut(s)	303, 744, 1355, 1414
BsaWI	WCCGGW	2 cut(s)	68, 216
BsaXI	ACNNNNNCTCC	7 cut(s)	25, 365, 395, 1134, 1164, 1836, 1866
Bsc4I	CCNNNNNNNGG	5 cut(s)	104, 927, 1231, 1265, 2266
Bse118I	RCCGGY	1 cut(s)	1721
BseBI	CCWGG	8 cut(s)	41, 99, 521, 1008, 1226, 1530, 1602, 1770
BseDI	CCNNGG	4 cut(s)	303, 744, 1355, 1414
BseLI	CCNNNNNNNGG	5 cut(s)	104, 927, 1231, 1265, 2266
BseMII	CTCAG	4 cut(s)	636, 864, 2107, 2183
BseSI	GKGCMC	1 cut(s)	614
BseXI	GCAGC	4 cut(s)	895, 940, 1478, 1496
BseYI	CCCAGC	1 cut(s)	1636
BsgI	GTGCAG	2 cut(s)	473, 1503
Bsh1236I	CGCG	1 cut(s)	697
BshFI	GGCC	6 cut(s)	114, 378, 855, 1609, 1721, 1998
BshNI	GGYRCC	2 cut(s)	137, 1772
BsiHKAI	GWGCWC	7 cut(s)	93, 338, 614, 778, 1594, 1688, 1941
BsiHKCI	CYCGRG	1 cut(s)	431
BsiSI	CCGG	5 cut(s)	69, 77, 105, 217, 1722
BslFI	GGGAC	2 cut(s)	700, 2160
BslI	CCNNNNNNNGG	5 cut(s)	104, 927, 1231, 1265, 2266
BsmAI	GTCTC	7 cut(s)	272, 293, 853, 1003, 1166, 1628, 2198
BsmBI	CGTCTC	1 cut(s)	2198
BsmFI	GGGAC	2 cut(s)	700, 2160
BsnI	GGCC	6 cut(s)	114, 378, 855, 1609, 1721, 1998
Bso31I	GGTCTC	2 cut(s)	272, 293
BsoBI	CYCGRG	1 cut(s)	431
Bsp1286I	GDGCHC	7 cut(s)	93, 338, 614, 778, 1594, 1688, 1941
Bsp1407I	TGTACA	1 cut(s)	1156
Bsp143I	GATC	5 cut(s)	101, 1360, 1546, 1577, 2105
BspACI	CCGC	6 cut(s)	144, 199, 409, 695, 1469, 1750
BspANI	GGCC	6 cut(s)	114, 378, 855, 1609, 1721, 1998
BspCNI	CTCAG	4 cut(s)	637, 863, 2106, 2182
BspFNI	CGCG	1 cut(s)	697
BspLI	GGNNCC	6 cut(s)	103, 139, 1362, 1651, 1720, 1774
BspPI	GGATC	5 cut(s)	96, 109, 1355, 1368, 1585
BspQI	GCTCTTC	2 cut(s)	343, 1946
BspT107I	GGYRCC	2 cut(s)	137, 1772
BspTNI	GGTCTC	2 cut(s)	272, 293
BsrBI	CCGCTC	1 cut(s)	409
BsrFI	RCCGGY	1 cut(s)	1721
BsrGI	TGTACA	1 cut(s)	1156
BssAI	RCCGGY	1 cut(s)	1721
BssECI	CCNNGG	4 cut(s)	303, 744, 1355, 1414
BssMI	GATC	5 cut(s)	101, 1360, 1546, 1577, 2105
BssNI	GRCGYC	3 cut(s)	108, 138, 689
BssT1I	CCWWGG	3 cut(s)	303, 744, 1414
Bst2UI	CCWGG	8 cut(s)	41, 99, 521, 1008, 1226, 1530, 1602, 1770
Bst4CI	ACNGT	7 cut(s)	7, 355, 780, 1712, 1852, 2122, 2191
Bst6I	CTCTTC	3 cut(s)	343, 1946, 2170
BstACI	GRCGYC	3 cut(s)	108, 138, 689
BstAPI	GCANNNNNTGC	2 cut(s)	782, 1278
BstAUI	TGTACA	1 cut(s)	1156
BstBAI	YACGTR	2 cut(s)	484, 910
BstC8I	GCNNGC	4 cut(s)	489, 918, 1658, 2000
BstDEI	CTNAG	5 cut(s)	645, 850, 922, 2093, 2169
BstDSI	CCRYGG	1 cut(s)	1355
BstFNI	CGCG	1 cut(s)	697
BstH2I	RGCGCY	1 cut(s)	141
BstHHI	GCGC	1 cut(s)	140
BstKTI	GATC	5 cut(s)	104, 1363, 1549, 1580, 2108
BstMAI	GTCTC	7 cut(s)	272, 293, 853, 1003, 1166, 1628, 2198
BstMBI	GATC	5 cut(s)	101, 1360, 1546, 1577, 2105
BstMWI	GCNNNNNNNGC	8 cut(s)	530, 782, 1235, 1244, 1278, 1567, 1637, 1823
BstNI	CCWGG	8 cut(s)	41, 99, 521, 1008, 1226, 1530, 1602, 1770
BstNSI	RCATGY	8 cut(s)	491, 550, 866, 920, 1285, 1617, 2011, 2039
BstSCI	CCNGG	8 cut(s)	39, 97, 519, 1006, 1224, 1528, 1600, 1768
BstSFI	CTRYAG	1 cut(s)	534
BstSLI	GKGCMC	1 cut(s)	614
BstSNI	TACGTA	1 cut(s)	910
BstUI	CGCG	1 cut(s)	697
BstV1I	GCAGC	4 cut(s)	895, 940, 1478, 1496
BstV2I	GAAGAC	4 cut(s)	163, 349, 1116, 1751
BstX2I	RGATCY	4 cut(s)	101, 1360, 1577, 2105
BstXI	CCANNNNNNTGG	2 cut(s)	830, 1826
BstYI	RGATCY	4 cut(s)	101, 1360, 1577, 2105
BsuI	GTATCC	1 cut(s)	742
BsuRI	GGCC	6 cut(s)	114, 378, 855, 1609, 1721, 1998
BtgI	CCRYGG	1 cut(s)	1355
BtsI	GCAGTG	2 cut(s)	978, 2223
Cac8I	GCNNGC	4 cut(s)	489, 918, 1658, 2000
CaiI	CAGNNNCTG	1 cut(s)	1231
CfoI	GCGC	1 cut(s)	140
Cfr10I	RCCGGY	1 cut(s)	1721
Cfr13I	GGNCC	3 cut(s)	376, 1564, 1719
CseI	GACGC	4 cut(s)	97, 247, 697, 2162
CsiI	ACCWGGT	2 cut(s)	39, 1600
Csp6I	GTAC	6 cut(s)	764, 907, 931, 1157, 1773, 2187
CspCI	CAANNNNNGTGG	2 cut(s)	2002, 2037
CviQI	GTAC	6 cut(s)	764, 907, 931, 1157, 1773, 2187
DdeI	CTNAG	5 cut(s)	645, 850, 922, 2093, 2169
DinI	GGCGCC	1 cut(s)	139
DpnI	GATC	5 cut(s)	103, 1362, 1548, 1579, 2107
DpnII	GATC	5 cut(s)	101, 1360, 1546, 1577, 2105
DraIII	CACNNNGTG	1 cut(s)	895
DrdI	GACNNNNNNGTC	1 cut(s)	1180
DseDI	GACNNNNNNGTC	1 cut(s)	1180
Eam1104I	CTCTTC	3 cut(s)	343, 1946, 2170
EarI	CTCTTC	3 cut(s)	343, 1946, 2170
Ecl136II	GAGCTC	1 cut(s)	1939
Eco105I	TACGTA	1 cut(s)	910
Eco130I	CCWWGG	3 cut(s)	303, 744, 1414
Eco147I	AGGCCT	2 cut(s)	855, 1998
Eco24I	GRGCYC	1 cut(s)	1941
Eco31I	GGTCTC	2 cut(s)	272, 293
Eco47I	GGWCC	1 cut(s)	1564
Eco53kI	GAGCTC	1 cut(s)	1939
Eco57I	CTGAAG	2 cut(s)	2058, 2201
Eco88I	CYCGRG	1 cut(s)	431
EcoICRI	GAGCTC	1 cut(s)	1939
EcoRII	CCWGG	8 cut(s)	39, 97, 519, 1006, 1224, 1528, 1600, 1768
EcoT14I	CCWWGG	3 cut(s)	303, 744, 1414
EcoT22I	ATGCAT	2 cut(s)	1274, 1765
EcoT38I	GRGCYC	1 cut(s)	1941
EgeI	GGCGCC	1 cut(s)	139
EheI	GGCGCC	1 cut(s)	139
ErhI	CCWWGG	3 cut(s)	303, 744, 1414
Esp3I	CGTCTC	1 cut(s)	2198
FaqI	GGGAC	2 cut(s)	700, 2160
FauI	CCCGC	1 cut(s)	1757
Fnu4HI	GCNGC	4 cut(s)	884, 954, 1467, 1485
FriOI	GRGCYC	1 cut(s)	1941
Fsp4HI	GCNGC	4 cut(s)	884, 954, 1467, 1485
FspBI	CTAG	1 cut(s)	2285
GlaI	GCGC	1 cut(s)	139
GluI	GCNGC	4 cut(s)	884, 954, 1467, 1485
GsaI	CCCAGC	1 cut(s)	1640
GsuI	CTGGAG	6 cut(s)	81, 296, 392, 1253, 1949, 2002
HaeII	RGCGCY	1 cut(s)	141
HaeIII	GGCC	6 cut(s)	114, 378, 855, 1609, 1721, 1998
HapII	CCGG	5 cut(s)	69, 77, 105, 217, 1722
HgaI	GACGC	4 cut(s)	97, 247, 697, 2162
HhaI	GCGC	1 cut(s)	140
Hin1I	GRCGYC	3 cut(s)	108, 138, 689
Hin6I	GCGC	1 cut(s)	138
HinP1I	GCGC	1 cut(s)	138
HindIII	AAGCTT	1 cut(s)	670
HinfI	GANTC	3 cut(s)	443, 1174, 1378
HpaII	CCGG	5 cut(s)	69, 77, 105, 217, 1722
HphI	GGTGA	4 cut(s)	329, 1214, 1841, 2116
Hpy166II	GTNNAC	9 cut(s)	49, 481, 612, 740, 907, 1457, 1678, 1889, 2187
Hpy188I	TCNGA	6 cut(s)	448, 1209, 1251, 1342, 2059, 2077
Hpy188III	TCNNGA	6 cut(s)	433, 566, 1259, 1663, 1872, 1928
Hpy8I	GTNNAC	9 cut(s)	49, 481, 612, 740, 907, 1457, 1678, 1889, 2187
Hpy99I	CGWCG	1 cut(s)	113
HpyAV	CCTTC	6 cut(s)	314, 371, 523, 594, 764, 2142
HpyCH4III	ACNGT	7 cut(s)	7, 355, 780, 1712, 1852, 2122, 2191
HpyCH4IV	ACGT	2 cut(s)	483, 909
HpyF10VI	GCNNNNNNNGC	8 cut(s)	530, 782, 1235, 1244, 1278, 1567, 1637, 1823
HpyF3I	CTNAG	5 cut(s)	645, 850, 922, 2093, 2169
HpySE526I	ACGT	2 cut(s)	483, 909
Hsp92I	GRCGYC	3 cut(s)	108, 138, 689
HspAI	GCGC	1 cut(s)	138
KasI	GGCGCC	1 cut(s)	137
KpnI	GGTACC	1 cut(s)	1776
Kzo9I	GATC	5 cut(s)	101, 1360, 1546, 1577, 2105
LguI	GCTCTTC	2 cut(s)	343, 1946
LmnI	GCTCC	5 cut(s)	773, 967, 1993, 2032, 2156
Lsp1109I	GCAGC	4 cut(s)	895, 940, 1478, 1496
LweI	GCATC	7 cut(s)	610, 895, 1084, 1259, 1750, 1761, 1772
MabI	ACCWGGT	2 cut(s)	39, 1600
MaeI	CTAG	1 cut(s)	2285
MaeII	ACGT	2 cut(s)	483, 909
MaeIII	GTNAC	5 cut(s)	7, 637, 1912, 1966, 2122
MalI	GATC	5 cut(s)	103, 1362, 1548, 1579, 2107
MbiI	CCGCTC	1 cut(s)	409
MboI	GATC	5 cut(s)	101, 1360, 1546, 1577, 2105
MflI	RGATCY	4 cut(s)	101, 1360, 1577, 2105
MhlI	GDGCHC	7 cut(s)	93, 338, 614, 778, 1594, 1688, 1941
MluCI	AATT	6 cut(s)	284, 729, 818, 1093, 1595, 1821
Mly113I	GGCGCC	1 cut(s)	138
MlyI	GAGTC	1 cut(s)	1168
Mph1103I	ATGCAT	2 cut(s)	1274, 1765
MroXI	GAANNNNTTC	1 cut(s)	1088
MseI	TTAA	5 cut(s)	222, 666, 1146, 1920, 2302
MslI	CAYNNNNRTG	2 cut(s)	293, 891
MspA1I	CMGCKG	4 cut(s)	199, 644, 953, 1469
MspI	CCGG	5 cut(s)	69, 77, 105, 217, 1722
MspR9I	CCNGG	8 cut(s)	41, 99, 521, 1008, 1226, 1530, 1602, 1770
MvaI	CCWGG	8 cut(s)	41, 99, 521, 1008, 1226, 1530, 1602, 1770
MvnI	CGCG	1 cut(s)	697
MwoI	GCNNNNNNNGC	8 cut(s)	530, 782, 1235, 1244, 1278, 1567, 1637, 1823
NarI	GGCGCC	1 cut(s)	138
NdeII	GATC	5 cut(s)	101, 1360, 1546, 1577, 2105
NlaIV	GGNNCC	6 cut(s)	103, 139, 1362, 1651, 1720, 1774
NmuCI	GTSAC	4 cut(s)	7, 637, 1912, 2122
NsiI	ATGCAT	2 cut(s)	1274, 1765
NspI	RCATGY	8 cut(s)	491, 550, 866, 920, 1285, 1617, 2011, 2039
PaeI	GCATGC	2 cut(s)	491, 920
PaeR7I	CTCGAG	1 cut(s)	431
PceI	AGGCCT	2 cut(s)	855, 1998
PciI	ACATGT	3 cut(s)	862, 1613, 2007
PciSI	GCTCTTC	2 cut(s)	343, 1946
PdmI	GAANNNNTTC	1 cut(s)	1088
PfeI	GAWTC	2 cut(s)	443, 1378
PflMI	CCANNNNNTGG	1 cut(s)	1231
PfoI	TCCNGGA	1 cut(s)	97
PkrI	GCNGC	4 cut(s)	885, 955, 1468, 1486
PleI	GAGTC	1 cut(s)	1168
PluTI	GGCGCC	1 cut(s)	141
PpsI	GAGTC	1 cut(s)	1168
Ppu21I	YACGTR	2 cut(s)	484, 910
PscI	ACATGT	3 cut(s)	862, 1613, 2007
PsiI	TTATAA	1 cut(s)	1401
Psp124BI	GAGCTC	1 cut(s)	1941
Psp6I	CCWGG	8 cut(s)	39, 97, 519, 1006, 1224, 1528, 1600, 1768
PspFI	CCCAGC	1 cut(s)	1636
PspGI	CCWGG	8 cut(s)	39, 97, 519, 1006, 1224, 1528, 1600, 1768
PspN4I	GGNNCC	6 cut(s)	103, 139, 1362, 1651, 1720, 1774
PspPI	GGNCC	3 cut(s)	376, 1564, 1719
PstNI	CAGNNNCTG	1 cut(s)	1231
PsuI	RGATCY	4 cut(s)	101, 1360, 1577, 2105
PvuII	CAGCTG	2 cut(s)	644, 953
RsaI	GTAC	6 cut(s)	765, 908, 932, 1158, 1774, 2188
RsaNI	GTAC	6 cut(s)	764, 907, 931, 1157, 1773, 2187
RseI	CAYNNNNRTG	2 cut(s)	293, 891
SacI	GAGCTC	1 cut(s)	1941
SapI	GCTCTTC	2 cut(s)	343, 1946
SaqAI	TTAA	5 cut(s)	222, 666, 1146, 1920, 2302
SatI	GCNGC	4 cut(s)	884, 954, 1467, 1485
Sau3AI	GATC	5 cut(s)	101, 1360, 1546, 1577, 2105
Sau96I	GGNCC	3 cut(s)	376, 1564, 1719
SchI	GAGTC	1 cut(s)	1168
ScrFI	CCNGG	8 cut(s)	41, 99, 521, 1008, 1226, 1530, 1602, 1770
SduI	GDGCHC	7 cut(s)	93, 338, 614, 778, 1594, 1688, 1941
SexAI	ACCWGGT	2 cut(s)	39, 1600
SfaNI	GCATC	7 cut(s)	610, 895, 1084, 1259, 1750, 1761, 1772
SfcI	CTRYAG	1 cut(s)	534
SfoI	GGCGCC	1 cut(s)	139
Sfr274I	CTCGAG	1 cut(s)	431
SinI	GGWCC	1 cut(s)	1564
SlaI	CTCGAG	1 cut(s)	431
SmiMI	CAYNNNNRTG	2 cut(s)	293, 891
SmlI	CTYRAG	3 cut(s)	431, 1661, 1958
SmoI	CTYRAG	3 cut(s)	431, 1661, 1958
SnaBI	TACGTA	1 cut(s)	910
SphI	GCATGC	2 cut(s)	491, 920
Sse9I	AATT	6 cut(s)	284, 729, 818, 1093, 1595, 1821
SseBI	AGGCCT	2 cut(s)	855, 1998
SsiI	CCGC	6 cut(s)	144, 199, 409, 695, 1469, 1750
SspDI	GGCGCC	1 cut(s)	137
SspMI	CTAG	1 cut(s)	2285
SstI	GAGCTC	1 cut(s)	1941
StuI	AGGCCT	2 cut(s)	855, 1998
StyD4I	CCNGG	8 cut(s)	39, 97, 519, 1006, 1224, 1528, 1600, 1768
StyI	CCWWGG	3 cut(s)	303, 744, 1414
TaaI	ACNGT	7 cut(s)	7, 355, 780, 1712, 1852, 2122, 2191
TaiI	ACGT	2 cut(s)	486, 912
TaqI	TCGA	3 cut(s)	432, 594, 1545
TasI	AATT	6 cut(s)	284, 729, 818, 1093, 1595, 1821
TatI	WGTACW	2 cut(s)	763, 1156
TfiI	GAWTC	2 cut(s)	443, 1378
Tru1I	TTAA	5 cut(s)	222, 666, 1146, 1920, 2302
Tru9I	TTAA	5 cut(s)	222, 666, 1146, 1920, 2302
TseFI	GTSAC	4 cut(s)	7, 637, 1912, 2122
TseI	GCWGC	4 cut(s)	883, 953, 1466, 1484
Tsp45I	GTSAC	4 cut(s)	7, 637, 1912, 2122
TspGWI	ACGGA	5 cut(s)	66, 374, 1281, 1577, 2222
Van91I	CCANNNNNTGG	1 cut(s)	1231
VneI	GTGCAC	1 cut(s)	610
VpaK11BI	GGWCC	1 cut(s)	1564
XapI	RAATTY	1 cut(s)	818
XceI	RCATGY	8 cut(s)	491, 550, 866, 920, 1285, 1617, 2011, 2039
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2026
XhoI	CTCGAG	1 cut(s)	431
XmnI	GAANNNNTTC	1 cut(s)	1088
XspI	CTAG	1 cut(s)	2285
Zsp2I	ATGCAT	2 cut(s)	1274, 1765

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.