AT5G04610

Methyltransferase-like protein 13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001235
Physical Location & Seq
Reverse (-)
1325588 .. 1326928
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04610.1

Sequence Viewer

Length: 1305 bp
ATGGCGCTTGATGTGAGAATCTTCGAGAAAACCTCTCCTTCTCGTTTCATCTCTTTCACTATCCCTAACCCTAATTCTCCGATTCATCTCCTCCGCGTCGCCGTCCTCGACTCGCCTGTTCATTCCACTGAGTCTTCTCCTCCCCGAGTCGCCGCCATTCTCGTCCCCAAGCACCGAGAATCCGATTGGATCTTCTCCACTGAGTCCGGTCAATTACAGCTTCTCCTTAACATGCCAGAAATCTCTCGATTGATCTTGATCGGTGATGATCACGATACTGACTCTGATTTACCTGCGGTTTATCACCGACCGAACGGCGAAGACGACGAATCTGAGAAACTCGAGATAAGATTGAAACCTCTTGTGGTAGCTTTGTTACCTAAAACCCTAACACGAGAAGAAATCGACGATGTGCCTTTTCTGATTTACGATGATAATGTTGTCTCCAGTGTAGAGATTGAAAAATGCGTCGGGCCTTTCGTTGGTGAAATGTTAATTGAAGATGTAGAGATTGAGATCGATGATGGAGTTAGAGAATTTAGGAGGAGATTGAGGTTTAAGAGAATGCCAAATTTAGTTCAATCTGCCATTAAAATCATTCCCAGAAGTTGTTCGAATCCTTCTCTGCCATTAATGGGAACAGAGTTCAAGCTTGATCTTACGGAACTTGTGCATCCTTACTTGGCACCAATGGTGGCAAGTCTTAGCTTGATTGGTTCTGATGTATATGATCATCTCAAATCTAAACCAAAGGCATTGTGCATTGGGGTTGGAGGTGGTGGATTGCTAAGTTTCTTAAGGCTACAGTTAGGGTTCGAAGTTACAGGCGTAGAGATAGATCCTGAAGTCTTGAGGATTGCAAGGCAATACTTCGGGTTAGAGGAAAGCTTAGCTCGTGTTCACGTTGAAGATGGTATTGAGTTCTTAAAGAGACTTTCTAAAAGCTGTGATGATGATGCCAGATTTGATGTCTTGATGGTTGATCTGGACTCTACTGATCCAATTCACGGCATGACTGCTCCTCCGGTTGAGTTTGTGGCTAAAGATGTGTTGCTAGCTGCAAGAACTGTTCTTGTTCCTTCTGGGGTTTTCATTATCAATGTCATTCCTCCGAACAAGACATTTTACCAAGAGCTTCAAGACCAGTTTAGACATGTCTTTGCCGAGTTGTATGAGATCGATGTAGGAAATGGTGAGAATTTTGTTCTCATCGCAACCGTTGCACCTAGAAAGAGCGGTTTCAACAGAGAGAATCTAACACCCGCTGTCTCGGGAAAGTATCTTGATGCTATACAGAAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synth

Protein Analysis

434

Amino Acids

48.49

Weight (kDa)

4.94

Isoelectric Point (pI)

55.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 249 - 386 3e-08 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 301
AccB1I GGYRCC 1 cut(s) 685
AccBSI CCGCTC 1 cut(s) 1236
AccII CGCG 1 cut(s) 96
AciI CCGC 5 cut(s) 94, 153, 296, 1236, 1263
AclWI GGATC 3 cut(s) 197, 833, 992
AcsI RAATTY 3 cut(s) 536, 571, 1198
AcuI CTGAAG 1 cut(s) 864
AfiI CCNNNNNNNGG 3 cut(s) 482, 635, 1007
AflII CTTAAG 1 cut(s) 796
AflIII ACRYGT 1 cut(s) 1153
AgsI TTSAA 8 cut(s) 355, 461, 500, 581, 649, 908, 1139, 1243
AluBI AGCT 9 cut(s) 220, 371, 652, 708, 888, 893, 945, 1058, 1135
AluI AGCT 9 cut(s) 220, 371, 652, 708, 888, 893, 945, 1058, 1135
Alw26I GTCTC 3 cut(s) 448, 925, 1273
AlwI GGATC 3 cut(s) 197, 833, 992
Ama87I CYCGRG 3 cut(s) 144, 341, 1270
AoxI GGCC 1 cut(s) 473
ApeKI GCWGC 1 cut(s) 1058
ApoI RAATTY 3 cut(s) 536, 571, 1198
AseI ATTAAT 1 cut(s) 632
Asp700I GAANNNNTTC 1 cut(s) 610
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 473
AsuHPI GGTGA 4 cut(s) 275, 296, 497, 1205
AsuII TTCGAA 2 cut(s) 614, 816
AsuNHI GCTAGC 1 cut(s) 1054
AvaI CYCGRG 3 cut(s) 144, 341, 1270
BanI GGYRCC 1 cut(s) 685
BauI CACGAG 2 cut(s) 393, 894
BbsI GAAGAC 2 cut(s) 126, 327
BbvI GCAGC 1 cut(s) 1045
BccI CCATC 3 cut(s) 518, 905, 970
BceAI ACGGC 3 cut(s) 86, 331, 1024
BclI TGATCA 2 cut(s) 268, 730
BcoDI GTCTC 3 cut(s) 448, 925, 1273
BfaI CTAG 2 cut(s) 1055, 1227
BfmI CTRYAG 1 cut(s) 803
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 796
BfuAI ACCTGC 1 cut(s) 301
BisI GCNGC 2 cut(s) 153, 1059
BlpI GCTNAGC 1 cut(s) 889
BlsI GCNGC 2 cut(s) 154, 1060
BmeT110I CYCGRG 3 cut(s) 144, 341, 1270
BmgT120I GGNCC 1 cut(s) 473
BmiI GGNNCC 1 cut(s) 687
BmsI GCATC 3 cut(s) 682, 946, 1276
BmtI GCTAGC 1 cut(s) 1058
BpiI GAAGAC 2 cut(s) 126, 327
BplI GAGNNNNNCTC 2 cut(s) 17, 49
BpmI CTGGAG 1 cut(s) 430
Bpu1102I GCTNAGC 1 cut(s) 889
Bpu14I TTCGAA 2 cut(s) 614, 816
BpuEI CTTGAG 1 cut(s) 871
Bsa29I ATCGAT 2 cut(s) 519, 1179
BsaBI GATNNNNATC 2 cut(s) 257, 515
BsaWI WCCGGW 2 cut(s) 206, 1024
BsaXI ACNNNNNCTCC 4 cut(s) 207, 237, 1006, 1036
Bsc4I CCNNNNNNNGG 3 cut(s) 482, 635, 1007
Bse1I ACTGG 2 cut(s) 447, 1144
Bse8I GATNNNNATC 2 cut(s) 257, 515
BseCI ATCGAT 2 cut(s) 519, 1179
BseGI GGATG 1 cut(s) 673
BseJI GATNNNNATC 2 cut(s) 257, 515
BseLI CCNNNNNNNGG 3 cut(s) 482, 635, 1007
BseMII CTCAG 3 cut(s) 120, 192, 324
BseNI ACTGG 2 cut(s) 447, 1144
BseRI GAGGAG 4 cut(s) 80, 129, 559, 1011
BseXI GCAGC 1 cut(s) 1045
Bsh1236I CGCG 1 cut(s) 96
Bsh1285I CGRYCG 1 cut(s) 311
BshFI GGCC 1 cut(s) 475
BshNI GGYRCC 1 cut(s) 685
BshVI ATCGAT 2 cut(s) 519, 1179
BsiEI CGRYCG 1 cut(s) 311
BsiHKCI CYCGRG 3 cut(s) 144, 341, 1270
BsiSI CCGG 2 cut(s) 207, 1025
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 3 cut(s) 482, 635, 1007
BsmAI GTCTC 3 cut(s) 448, 925, 1273
BsmFI GGGAC 1 cut(s) 149
BsmI GAATGC 1 cut(s) 570
BsnI GGCC 1 cut(s) 475
BsoBI CYCGRG 3 cut(s) 144, 341, 1270
Bsp119I TTCGAA 2 cut(s) 614, 816
Bsp1720I GCTNAGC 1 cut(s) 889
BspACI CCGC 5 cut(s) 94, 153, 296, 1236, 1263
BspANI GGCC 1 cut(s) 475
BspCNI CTCAG 3 cut(s) 121, 193, 325
BspDI ATCGAT 2 cut(s) 519, 1179
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 1 cut(s) 687
BspMI ACCTGC 1 cut(s) 301
BspOI GCTAGC 1 cut(s) 1058
BspPI GGATC 3 cut(s) 197, 833, 992
BspT104I TTCGAA 2 cut(s) 614, 816
BspT107I GGYRCC 1 cut(s) 685
BspTI CTTAAG 1 cut(s) 796
BsrBI CCGCTC 1 cut(s) 1236
BsrI ACTGG 2 cut(s) 447, 1144
BssSI CACGAG 2 cut(s) 393, 894
Bst2BI CACGAG 2 cut(s) 393, 894
Bst4CI ACNGT 3 cut(s) 807, 1069, 1219
BstAFI CTTAAG 1 cut(s) 796
BstAPI GCANNNNNTGC 1 cut(s) 1220
BstBI TTCGAA 2 cut(s) 614, 816
BstC8I GCNNGC 1 cut(s) 1056
BstDEI CTNAG 6 cut(s) 129, 201, 333, 704, 788, 889
BstF5I GGATG 1 cut(s) 673
BstFNI CGCG 1 cut(s) 96
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstMAI GTCTC 3 cut(s) 448, 925, 1273
BstMCI CGRYCG 1 cut(s) 311
BstMWI GCNNNNNNNGC 1 cut(s) 1220
BstNSI RCATGY 2 cut(s) 235, 1157
BstSFI CTRYAG 1 cut(s) 803
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 1 cut(s) 1045
BstV2I GAAGAC 2 cut(s) 126, 327
BstX2I RGATCY 2 cut(s) 189, 838
BstYI RGATCY 2 cut(s) 189, 838
Bsu15I ATCGAT 2 cut(s) 519, 1179
BsuRI GGCC 1 cut(s) 475
BsuTUI ATCGAT 2 cut(s) 519, 1179
BtgZI GCGATG 1 cut(s) 1195
BtsCI GGATG 1 cut(s) 673
BtsIMutI CAGTG 3 cut(s) 126, 198, 454
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 1 cut(s) 1056
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 473
ClaI ATCGAT 2 cut(s) 519, 1179
CseI GACGC 2 cut(s) 85, 457
CviAII CATG 3 cut(s) 232, 1012, 1154
DdeI CTNAG 6 cut(s) 129, 201, 333, 704, 788, 889
Eco57I CTGAAG 1 cut(s) 864
Eco88I CYCGRG 3 cut(s) 144, 341, 1270
FaeI CATG 3 cut(s) 235, 1015, 1157
FaiI YATR 7 cut(s) 233, 727, 729, 1013, 1155, 1173, 1292
FaqI GGGAC 1 cut(s) 149
FatI CATG 3 cut(s) 231, 1011, 1153
FauI CCCGC 1 cut(s) 1270
FbaI TGATCA 2 cut(s) 268, 730
Fnu4HI GCNGC 2 cut(s) 153, 1059
FokI GGATG 1 cut(s) 660
Fsp4HI GCNGC 2 cut(s) 153, 1059
FspBI CTAG 2 cut(s) 1055, 1227
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 153, 1059
GsuI CTGGAG 1 cut(s) 430
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 475
HapII CCGG 2 cut(s) 207, 1025
HgaI GACGC 2 cut(s) 85, 457
HhaI GCGC 1 cut(s) 7
Hin1II CATG 3 cut(s) 235, 1015, 1157
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HindIII AAGCTT 2 cut(s) 650, 886
HpaII CCGG 2 cut(s) 207, 1025
HphI GGTGA 4 cut(s) 275, 296, 497, 1205
Hpy166II GTNNAC 1 cut(s) 901
Hpy188I TCNGA 8 cut(s) 81, 184, 286, 334, 423, 721, 1113, 1304
Hpy8I GTNNAC 1 cut(s) 901
Hpy99I CGWCG 4 cut(s) 101, 329, 410, 473
HpyAV CCTTC 3 cut(s) 48, 630, 1089
HpyCH4III ACNGT 3 cut(s) 807, 1069, 1219
HpyCH4IV ACGT 1 cut(s) 903
HpyCH4V TGCA 5 cut(s) 673, 762, 860, 1061, 1223
HpyF10VI GCNNNNNNNGC 1 cut(s) 1220
HpyF3I CTNAG 6 cut(s) 129, 201, 333, 704, 788, 889
HpySE526I ACGT 1 cut(s) 903
Hsp92II CATG 3 cut(s) 235, 1015, 1157
HspAI GCGC 1 cut(s) 5
Ksp22I TGATCA 2 cut(s) 268, 730
LmnI GCTCC 1 cut(s) 1024
Lsp1109I GCAGC 1 cut(s) 1045
LweI GCATC 3 cut(s) 682, 946, 1276
MaeI CTAG 2 cut(s) 1055, 1227
MaeII ACGT 1 cut(s) 903
MaeIII GTNAC 2 cut(s) 375, 820
MbiI CCGCTC 1 cut(s) 1236
MboII GAAGA 7 cut(s) 13, 126, 184, 332, 410, 512, 920
MflI RGATCY 2 cut(s) 189, 838
MluCI AATT 7 cut(s) 73, 212, 495, 536, 571, 1002, 1198
MlyI GAGTC 6 cut(s) 104, 140, 156, 212, 275, 983
MmeI TCCRAC 1 cut(s) 751
MroXI GAANNNNTTC 1 cut(s) 610
MseI TTAA 7 cut(s) 228, 494, 558, 591, 632, 797, 926
MspA1I CMGCKG 1 cut(s) 1265
MspCI CTTAAG 1 cut(s) 796
MspI CCGG 2 cut(s) 207, 1025
Mva1269I GAATGC 1 cut(s) 570
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 1 cut(s) 1220
NheI GCTAGC 1 cut(s) 1054
NlaIII CATG 3 cut(s) 235, 1015, 1157
NlaIV GGNNCC 1 cut(s) 687
NmeAIII GCCGAG 1 cut(s) 1189
NspI RCATGY 2 cut(s) 235, 1157
NspV TTCGAA 2 cut(s) 614, 816
PaeR7I CTCGAG 1 cut(s) 341
PciI ACATGT 1 cut(s) 1153
PcsI WCGNNNNNNNCGW 3 cut(s) 105, 321, 477
PctI GAATGC 1 cut(s) 570
PdmI GAANNNNTTC 1 cut(s) 610
PfeI GAWTC 6 cut(s) 18, 82, 179, 329, 616, 1252
PkrI GCNGC 2 cut(s) 154, 1060
PleI GAGTC 6 cut(s) 104, 139, 155, 211, 275, 983
PpsI GAGTC 6 cut(s) 104, 139, 155, 211, 275, 983
PscI ACATGT 1 cut(s) 1153
PshBI ATTAAT 1 cut(s) 632
PspN4I GGNNCC 1 cut(s) 687
PspPI GGNCC 1 cut(s) 473
PsuI RGATCY 2 cut(s) 189, 838
SaqAI TTAA 7 cut(s) 228, 494, 558, 591, 632, 797, 926
SatI GCNGC 2 cut(s) 153, 1059
Sau96I GGNCC 1 cut(s) 473
SchI GAGTC 6 cut(s) 104, 140, 156, 212, 275, 983
SfaNI GCATC 3 cut(s) 682, 946, 1276
SfcI CTRYAG 1 cut(s) 803
Sfr274I CTCGAG 1 cut(s) 341
SfuI TTCGAA 2 cut(s) 614, 816
SlaI CTCGAG 1 cut(s) 341
SmlI CTYRAG 3 cut(s) 341, 796, 850
SmoI CTYRAG 3 cut(s) 341, 796, 850
Sse9I AATT 7 cut(s) 73, 212, 495, 536, 571, 1002, 1198
SsiI CCGC 5 cut(s) 94, 153, 296, 1236, 1263
SspMI CTAG 2 cut(s) 1055, 1227
TaaI ACNGT 3 cut(s) 807, 1069, 1219
TaiI ACGT 1 cut(s) 906
TaqI TCGA 9 cut(s) 24, 108, 247, 342, 405, 519, 614, 816, 1179
TaqII GACCGA 1 cut(s) 325
TasI AATT 7 cut(s) 73, 212, 495, 536, 571, 1002, 1198
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 6 cut(s) 18, 82, 179, 329, 616, 1252
Tru1I TTAA 7 cut(s) 228, 494, 558, 591, 632, 797, 926
Tru9I TTAA 7 cut(s) 228, 494, 558, 591, 632, 797, 926
TscAI CASTG 3 cut(s) 133, 205, 454
TseI GCWGC 1 cut(s) 1058
TspDTI ATGAA 4 cut(s) 37, 74, 110, 1081
TspGWI ACGGA 1 cut(s) 677
TspRI CASTG 3 cut(s) 133, 205, 454
Vha464I CTTAAG 1 cut(s) 796
VspI ATTAAT 1 cut(s) 632
XapI RAATTY 3 cut(s) 536, 571, 1198
XceI RCATGY 2 cut(s) 235, 1157
XhoI CTCGAG 1 cut(s) 341
XmnI GAANNNNTTC 1 cut(s) 610
XspI CTAG 2 cut(s) 1055, 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.