Rroxscaffold_1G00028300

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001235
Physical Location & Seq
Reverse (-)
36021765 .. 36022642
878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00028300.1

Sequence Viewer

Length: 552 bp
ATGTTAAACCCATCTCGTTTCACTACCTTCACTTTCCCAAACCCTTCCCACTCCACCACCGCCTCACCCTCTCGCGTCGCCGTTCTCGACTCGCCTTCCCAACCTCCTCGCCGACCCACCCCGAGTCACCGCAGCGTGCTCGTCCCCAAACACTTCCACTCCGACCGGATATTCTCCACCCACTCCGGCCATCTCCACCTCCTCCTCGCCCTCCCCCGGCATCTTCCGCCCGATCCTCGTCGGCGACAGCTCTCACGACTCACCTCCAATCTACCGCGGTCCTCTCAAGACGACGACATGTCGTGTGACGACGAACTCGAGGTAGAGGCTAGTCGTGACGTGGGCAGAGCCAATGATGTTAATACTAAGTTTGATGTGATTATGTTTGATTTGGATTCAAGGGATGTTACGGATGGTCTAATTGCTTCCCCATTGGAGTTTGTTAAGAAGCATGTCCTTTTGTCGCCCGATCAATTCTTCCGTGATAATGGAATCCTAGTTATAAATGTGATTCCTCCGTATACATCATTTTTACAAGACATTGGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synth

Protein Analysis

183

Amino Acids

20.6

Weight (kDa)

6.49

Isoelectric Point (pI)

67.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 503
AccI GTMKAC 1 cut(s) 521
AccII CGCG 2 cut(s) 75, 277
AciI CCGC 5 cut(s) 60, 130, 227, 275, 277
AclWI GGATC 1 cut(s) 227
AcoI YGGCCR 1 cut(s) 187
AfiI CCNNNNNNNGG 1 cut(s) 216
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 1 cut(s) 399
AhdI GACNNNNNGTC 1 cut(s) 298
AjiI CACGTC 1 cut(s) 340
AluBI AGCT 1 cut(s) 250
AluI AGCT 1 cut(s) 250
Alw21I GWGCWC 1 cut(s) 141
AlwI GGATC 1 cut(s) 227
Ama87I CYCGRG 2 cut(s) 121, 317
AoxI GGCC 1 cut(s) 187
ApeKI GCWGC 1 cut(s) 132
AspS9I GGNCC 2 cut(s) 279, 545
AsuC2I CCSGG 1 cut(s) 217
AsuHPI GGTGA 3 cut(s) 57, 119, 253
AvaI CYCGRG 2 cut(s) 121, 317
AvaII GGWCC 2 cut(s) 279, 545
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 144
BccI CCATC 3 cut(s) 19, 198, 407
BceAI ACGGC 1 cut(s) 65
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BcnI CCSGG 1 cut(s) 217
BfaI CTAG 2 cut(s) 330, 497
BisI GCNGC 1 cut(s) 133
BlsI GCNGC 1 cut(s) 134
Bme1390I CCNGG 1 cut(s) 217
Bme18I GGWCC 2 cut(s) 279, 545
BmeRI GACNNNNNGTC 1 cut(s) 298
BmeT110I CYCGRG 2 cut(s) 121, 317
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 2 cut(s) 279, 545
BmrFI CCNGG 1 cut(s) 217
BmsI GCATC 1 cut(s) 229
BoxI GACNNNNGTC 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 217
BsaJI CCNNGG 2 cut(s) 215, 275
BsaWI WCCGGW 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 216
BseDI CCNNGG 2 cut(s) 215, 275
BseGI GGATG 2 cut(s) 409, 418
BseLI CCNNNNNNNGG 1 cut(s) 216
BseRI GAGGAG 3 cut(s) 96, 191, 194
BseXI GCAGC 1 cut(s) 144
Bsh1236I CGCG 2 cut(s) 75, 277
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 1 cut(s) 189
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 141
BsiHKCI CYCGRG 2 cut(s) 121, 317
BsiSI CCGG 3 cut(s) 166, 186, 217
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 216
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 1 cut(s) 189
BsoBI CYCGRG 2 cut(s) 121, 317
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 2 cut(s) 232, 469
BspACI CCGC 5 cut(s) 60, 130, 227, 275, 277
BspANI GGCC 1 cut(s) 189
BspFNI CGCG 2 cut(s) 75, 277
BspPI GGATC 1 cut(s) 227
BssECI CCNNGG 2 cut(s) 215, 275
BssMI GATC 2 cut(s) 232, 469
BssNAI GTATAC 1 cut(s) 522
Bst1107I GTATAC 1 cut(s) 522
BstC8I GCNNGC 1 cut(s) 137
BstDEI CTNAG 1 cut(s) 366
BstDSI CCRYGG 1 cut(s) 275
BstF5I GGATG 2 cut(s) 409, 418
BstFNI CGCG 2 cut(s) 75, 277
BstKTI GATC 2 cut(s) 235, 472
BstMBI GATC 2 cut(s) 232, 469
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNSI RCATGY 2 cut(s) 301, 455
BstPAI GACNNNNGTC 1 cut(s) 543
BstSCI CCNGG 1 cut(s) 215
BstUI CGCG 2 cut(s) 75, 277
BstV1I GCAGC 1 cut(s) 144
BstZ17I GTATAC 1 cut(s) 522
BsuRI GGCC 1 cut(s) 189
BtgI CCRYGG 1 cut(s) 275
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 2 cut(s) 409, 418
Cac8I GCNNGC 1 cut(s) 137
Cfr13I GGNCC 2 cut(s) 279, 545
Cfr42I CCGCGG 1 cut(s) 278
CseI GACGC 1 cut(s) 64
CviAII CATG 3 cut(s) 298, 452, 549
CviJI RGCY 4 cut(s) 189, 250, 329, 350
CviKI_1 RGCY 4 cut(s) 189, 250, 329, 350
DdeI CTNAG 1 cut(s) 366
DpnI GATC 2 cut(s) 234, 471
DpnII GATC 2 cut(s) 232, 469
DriI GACNNNNNGTC 1 cut(s) 298
EaeI YGGCCR 1 cut(s) 187
Eam1105I GACNNNNNGTC 1 cut(s) 298
EciI GGCGGA 1 cut(s) 216
Eco47I GGWCC 2 cut(s) 279, 545
Eco88I CYCGRG 2 cut(s) 121, 317
FaeI CATG 3 cut(s) 301, 455, 552
FaiI YATR 6 cut(s) 299, 383, 453, 503, 522, 550
FaqI GGGAC 1 cut(s) 128
FatI CATG 3 cut(s) 297, 451, 548
FblI GTMKAC 1 cut(s) 521
Fnu4HI GCNGC 1 cut(s) 133
FokI GGATG 2 cut(s) 416, 425
Fsp4HI GCNGC 1 cut(s) 133
FspBI CTAG 2 cut(s) 330, 497
GluI GCNGC 1 cut(s) 133
HaeIII GGCC 1 cut(s) 189
HapII CCGG 3 cut(s) 166, 186, 217
HgaI GACGC 1 cut(s) 64
Hin1II CATG 3 cut(s) 301, 455, 552
HinfI GANTC 6 cut(s) 89, 124, 258, 395, 492, 511
HpaII CCGG 3 cut(s) 166, 186, 217
HphI GGTGA 3 cut(s) 57, 119, 253
Hpy166II GTNNAC 1 cut(s) 522
Hpy188I TCNGA 1 cut(s) 163
Hpy188III TCNNGA 4 cut(s) 86, 255, 287, 335
Hpy8I GTNNAC 1 cut(s) 522
Hpy99I CGWCG 4 cut(s) 80, 243, 296, 314
HpyAV CCTTC 3 cut(s) 37, 54, 105
HpyCH4IV ACGT 1 cut(s) 339
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 1 cut(s) 366
HpySE526I ACGT 1 cut(s) 339
Hsp92II CATG 3 cut(s) 301, 455, 552
KspI CCGCGG 1 cut(s) 278
Kzo9I GATC 2 cut(s) 232, 469
LpnPI CCDG 3 cut(s) 179, 199, 230
Lsp1109I GCAGC 1 cut(s) 144
LweI GCATC 1 cut(s) 229
MaeI CTAG 2 cut(s) 330, 497
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 4 cut(s) 125, 305, 335, 406
MalI GATC 2 cut(s) 234, 471
MboI GATC 2 cut(s) 232, 469
MboII GAAGA 2 cut(s) 215, 469
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 2 cut(s) 420, 473
MlyI GAGTC 3 cut(s) 83, 133, 252
MmeI TCCRAC 1 cut(s) 186
MseI TTAA 3 cut(s) 5, 360, 444
MspA1I CMGCKG 1 cut(s) 277
MspI CCGG 3 cut(s) 166, 186, 217
MspR9I CCNGG 1 cut(s) 217
MvnI CGCG 2 cut(s) 75, 277
MwoI GCNNNNNNNGC 1 cut(s) 226
NciI CCSGG 1 cut(s) 217
NdeII GATC 2 cut(s) 232, 469
NlaIII CATG 3 cut(s) 301, 455, 552
NmuCI GTSAC 3 cut(s) 125, 305, 335
NspI RCATGY 2 cut(s) 301, 455
PaeR7I CTCGAG 1 cut(s) 317
PciI ACATGT 1 cut(s) 297
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 315
PfeI GAWTC 3 cut(s) 395, 492, 511
PkrI GCNGC 1 cut(s) 134
PleI GAGTC 3 cut(s) 83, 132, 252
PpsI GAGTC 3 cut(s) 83, 132, 252
PscI ACATGT 1 cut(s) 297
PshAI GACNNNNGTC 1 cut(s) 543
PsiI TTATAA 1 cut(s) 503
PspPI GGNCC 2 cut(s) 279, 545
PspXI VCTCGAGB 1 cut(s) 317
SacII CCGCGG 1 cut(s) 278
SaqAI TTAA 3 cut(s) 5, 360, 444
SatI GCNGC 1 cut(s) 133
Sau3AI GATC 2 cut(s) 232, 469
Sau96I GGNCC 2 cut(s) 279, 545
SchI GAGTC 3 cut(s) 83, 133, 252
ScrFI CCNGG 1 cut(s) 217
SduI GDGCHC 1 cut(s) 141
SetI ASST 7 cut(s) 29, 106, 201, 252, 266, 324, 342
SfaNI GCATC 1 cut(s) 229
Sfr274I CTCGAG 1 cut(s) 317
Sfr303I CCGCGG 1 cut(s) 278
SgrBI CCGCGG 1 cut(s) 278
SinI GGWCC 2 cut(s) 279, 545
SlaI CTCGAG 1 cut(s) 317
SmlI CTYRAG 2 cut(s) 285, 317
SmoI CTYRAG 2 cut(s) 285, 317
Sse9I AATT 2 cut(s) 420, 473
SsiI CCGC 5 cut(s) 60, 130, 227, 275, 277
SspMI CTAG 2 cut(s) 330, 497
StyD4I CCNGG 1 cut(s) 215
TaiI ACGT 1 cut(s) 342
TaqI TCGA 2 cut(s) 87, 318
TasI AATT 2 cut(s) 420, 473
TfiI GAWTC 3 cut(s) 395, 492, 511
Tru1I TTAA 3 cut(s) 5, 360, 444
Tru9I TTAA 3 cut(s) 5, 360, 444
TseFI GTSAC 3 cut(s) 125, 305, 335
TseI GCWGC 1 cut(s) 132
Tsp45I GTSAC 3 cut(s) 125, 305, 335
TspGWI ACGGA 3 cut(s) 425, 470, 507
VpaK11BI GGWCC 2 cut(s) 279, 545
XceI RCATGY 2 cut(s) 301, 455
XhoI CTCGAG 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 521
XspI CTAG 2 cut(s) 330, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.