Rroxscaffold_4G00317000

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001235
Physical Location & Seq
Forward (+)
41968066 .. 41973126
5061 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00317000.1

Sequence Viewer

Length: 1359 bp
ATGGCTATCCTCGATGAGACGACTTTCGAAATGTTAAACCCATCTCGTTTCACTACCTTCACTTTCCCAAACCCTTGCGACTCCACCACCACCTCACTCCTCCGCGTCGCCGTTCTCGACTCGCCTTTCCAACCCCCCGCCGACCCACCCCGAGTCGCCGCAATGCTCGTCCCCAAACACCGCCACTCCGACTGGATATTCTCCACCCACTCCGGCCATCTCCACCTCCTCCTCAGCTCCCCCGGCATCTCCCGCCTGATCCTCATTGGCGACAGCTCTCACGACTCGCCCCCAATCTACCGCCGGCCGCTCAAAGACGACGAACTCGCGGTGAGCCTGAAACCTCTCTTCCTTGCTTTATCTCCCAAGTCCTGCTTTAAACACGGCATTCCTGAGATACCCATTTTGAGTTACCAAGATAATTTGATTTCTAGTTTGTTTTTGGAGAAATGCGTTGGGAGTTTGGTTGGTGAAATGGTGGTGGAGGATGTGGAGATTGAGTGTGGTGGTGAGGTTTCAACGAGGGAGTTCAGGAGGCGTTTGAGGTTTAAAAGAATGCCCAATTTGATTCAGACGGAGGTGCGTATTGTTCCCAAAATGGGTTTTGGTATGGATTGTGTGGGAATTGGACAAGTTGAGTTTAGGCTAGATGATAGTGTTTTAGTGCACTCTTATTTGGTTCCTATGGTGGCAAGTCTCAGCCTGGCTGCTCCTTACATTGAGGGTCGGATTCAAAGTGGGGTAAGGCCAAAAGCTTTGTGTTTAGGAGTTGGGGGTGGAGCTTTGCTTGGTTTCTTGAGAACCCAATTTGGTTTTCAGGTTGTGGGTGTGGAAGCCGATGAGGCAGTGCTAAGTGTTTCTAGGCGGTATTTTGGGCTGGAAGATGGTGAGTACATCAAAGTTTGTGTTGGAGATGCATTGGAATTTATAGATAAACTTGCTGGTGCCTGCGAGGTAGAGGCTAGCTGTGACGTGGGCAGAGGCAATGATGTTAATACTAAGTTTGATGTGATTATGGTTGATTTGGATTCAAGTGATGCTAGGGATGGTCTAATTGCTCCACCATTGGAGTTTGTTCAGAAGCATGTCCTTTTGTCAGCCAGATCAATTCTCTCTGAAAATGGAATCCTAGCTATAAATGTGATTCCTCCAGATACATCATTTTACAAGGCATTGGTCCATGAGTTTCGAGATGTTTTTAATGACTTATACGAAATAGATGTGGGAAATGGGGAAAATTTTATTCTTATTGCTGTGGCATCGCCTCTCATGCCTTCTAGTGATTGCGAGAACTGTTTCCTCAACAAACTAGGAAATGCTATATCGGGGGCATACTTGAATTCCGTAAAGAAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synth

Protein Analysis

452

Amino Acids

49.54

Weight (kDa)

5.11

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 944
AccBSI CCGCTC 1 cut(s) 310
AccII CGCG 2 cut(s) 105, 329
AciI CCGC 9 cut(s) 103, 138, 159, 181, 253, 301, 308, 329, 865
AclWI GGATC 1 cut(s) 253
AcoI YGGCCR 2 cut(s) 214, 305
AcsI RAATTY 3 cut(s) 923, 1237, 1339
AfaI GTAC 1 cut(s) 893
AfiI CCNNNNNNNGG 1 cut(s) 599
AgsI TTSAA 4 cut(s) 519, 734, 1032, 1339
AjiI CACGTC 1 cut(s) 973
AjnI CCWGG 1 cut(s) 702
AjuI GAANNNNNNNTTGG 2 cut(s) 798, 830
AluBI AGCT 6 cut(s) 237, 276, 755, 782, 966, 1133
AluI AGCT 6 cut(s) 237, 276, 755, 782, 966, 1133
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 2 cut(s) 11, 701
Alw44I GTGCAC 1 cut(s) 665
AlwI GGATC 1 cut(s) 253
Ama87I CYCGRG 1 cut(s) 150
AoxI GGCC 3 cut(s) 214, 305, 746
ApaLI GTGCAC 1 cut(s) 665
ApeKI GCWGC 1 cut(s) 707
ApoI RAATTY 3 cut(s) 923, 1237, 1339
Asp700I GAANNNNTTC 1 cut(s) 1295
AspS9I GGNCC 1 cut(s) 1177
AsuC2I CCSGG 1 cut(s) 243
AsuHPI GGTGA 4 cut(s) 343, 482, 521, 899
AsuII TTCGAA 1 cut(s) 27
AsuNHI GCTAGC 1 cut(s) 962
AvaI CYCGRG 1 cut(s) 150
AvaII GGWCC 1 cut(s) 1177
BaeGI GKGCMC 1 cut(s) 669
BanI GGYRCC 1 cut(s) 944
Bbv12I GWGCWC 1 cut(s) 669
BbvCI CCTCAGC 1 cut(s) 233
BbvI GCAGC 1 cut(s) 694
BccI CCATC 4 cut(s) 49, 225, 878, 1040
BceAI ACGGC 2 cut(s) 95, 400
BciT130I CCWGG 1 cut(s) 704
BcnI CCSGG 1 cut(s) 243
BcoDI GTCTC 2 cut(s) 11, 701
BfaI CTAG 8 cut(s) 432, 647, 861, 963, 1041, 1130, 1278, 1310
BglI GCCNNNNNGGC 1 cut(s) 842
BglII AGATCT 1 cut(s) 1353
BisI GCNGC 3 cut(s) 159, 308, 708
BlsI GCNGC 3 cut(s) 160, 309, 709
Bme1390I CCNGG 2 cut(s) 243, 704
Bme18I GGWCC 1 cut(s) 1177
BmeT110I CYCGRG 1 cut(s) 150
BmgBI CACGTC 1 cut(s) 973
BmgT120I GGNCC 1 cut(s) 1177
BmiI GGNNCC 2 cut(s) 681, 946
BmrFI CCNGG 2 cut(s) 243, 704
BmsI GCATC 4 cut(s) 255, 904, 1027, 1268
BmtI GCTAGC 1 cut(s) 966
BpmI CTGGAG 1 cut(s) 1134
Bpu10I CCTNAGC 1 cut(s) 233
Bpu14I TTCGAA 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 817
BpuMI CCSGG 1 cut(s) 243
BsaJI CCNNGG 1 cut(s) 241
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 485, 515
Bsc4I CCNNNNNNNGG 1 cut(s) 599
Bse118I RCCGGY 1 cut(s) 303
Bse1I ACTGG 1 cut(s) 197
Bse3DI GCAATG 2 cut(s) 168, 991
BseBI CCWGG 1 cut(s) 704
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 2 cut(s) 493, 1051
BseLI CCNNNNNNNGG 1 cut(s) 599
BseMI GCAATG 2 cut(s) 168, 991
BseMII CTCAG 3 cut(s) 247, 384, 712
BseNI ACTGG 1 cut(s) 197
BseRI GAGGAG 3 cut(s) 89, 218, 221
BseSI GKGCMC 1 cut(s) 669
BseX3I CGGCCG 1 cut(s) 305
BseXI GCAGC 1 cut(s) 694
Bsh1236I CGCG 2 cut(s) 105, 329
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 3 cut(s) 216, 307, 748
BshNI GGYRCC 1 cut(s) 944
BsiEI CGRYCG 1 cut(s) 308
BsiHKAI GWGCWC 1 cut(s) 669
BsiHKCI CYCGRG 1 cut(s) 150
BsiSI CCGG 3 cut(s) 213, 243, 304
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 599
BsmAI GTCTC 2 cut(s) 11, 701
BsmBI CGTCTC 1 cut(s) 11
BsmFI GGGAC 1 cut(s) 155
BsmI GAATGC 2 cut(s) 387, 561
BsnI GGCC 3 cut(s) 216, 307, 748
BsoBI CYCGRG 1 cut(s) 150
Bsp119I TTCGAA 1 cut(s) 27
Bsp1286I GDGCHC 1 cut(s) 669
Bsp143I GATC 3 cut(s) 258, 1103, 1353
BspACI CCGC 9 cut(s) 103, 138, 159, 181, 253, 301, 308, 329, 865
BspANI GGCC 3 cut(s) 216, 307, 748
BspCNI CTCAG 3 cut(s) 246, 385, 711
BspFNI CGCG 2 cut(s) 105, 329
BspLI GGNNCC 2 cut(s) 681, 946
BspOI GCTAGC 1 cut(s) 966
BspPI GGATC 1 cut(s) 253
BspT104I TTCGAA 1 cut(s) 27
BspT107I GGYRCC 1 cut(s) 944
BsrBI CCGCTC 1 cut(s) 310
BsrDI GCAATG 2 cut(s) 168, 991
BsrFI RCCGGY 1 cut(s) 303
BsrI ACTGG 1 cut(s) 197
BssAI RCCGGY 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 3 cut(s) 258, 1103, 1353
Bst2UI CCWGG 1 cut(s) 704
Bst4CI ACNGT 1 cut(s) 1295
Bst6I CTCTTC 1 cut(s) 353
BstBI TTCGAA 1 cut(s) 27
BstC8I GCNNGC 3 cut(s) 305, 949, 964
BstDEI CTNAG 5 cut(s) 233, 393, 698, 851, 999
BstF5I GGATG 2 cut(s) 493, 1051
BstFNI CGCG 2 cut(s) 105, 329
BstKTI GATC 3 cut(s) 261, 1106, 1356
BstMAI GTCTC 2 cut(s) 11, 701
BstMBI GATC 3 cut(s) 258, 1103, 1353
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 4 cut(s) 243, 252, 842, 1270
BstNI CCWGG 1 cut(s) 704
BstNSI RCATGY 1 cut(s) 1088
BstSCI CCNGG 2 cut(s) 241, 702
BstSLI GKGCMC 1 cut(s) 669
BstUI CGCG 2 cut(s) 105, 329
BstV1I GCAGC 1 cut(s) 694
BstX2I RGATCY 1 cut(s) 1353
BstYI RGATCY 1 cut(s) 1353
BstZI CGGCCG 1 cut(s) 305
BsuRI GGCC 3 cut(s) 216, 307, 748
BtgZI GCGATG 1 cut(s) 1245
BtrI CACGTC 1 cut(s) 973
BtsCI GGATG 2 cut(s) 493, 1051
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 1 cut(s) 852
Cac8I GCNNGC 3 cut(s) 305, 949, 964
Cfr10I RCCGGY 1 cut(s) 303
Cfr13I GGNCC 1 cut(s) 1177
CseI GACGC 1 cut(s) 94
Csp6I GTAC 1 cut(s) 892
CviAII CATG 3 cut(s) 1085, 1181, 1270
CviQI GTAC 1 cut(s) 892
DdeI CTNAG 5 cut(s) 233, 393, 698, 851, 999
DpnI GATC 3 cut(s) 260, 1105, 1355
DpnII GATC 3 cut(s) 258, 1103, 1353
DraI TTTAAA 2 cut(s) 379, 550
EaeI YGGCCR 2 cut(s) 214, 305
EagI CGGCCG 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EclXI CGGCCG 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 1177
Eco52I CGGCCG 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 150
EcoRI GAATTC 1 cut(s) 1339
EcoRII CCWGG 1 cut(s) 702
EcoT22I ATGCAT 1 cut(s) 919
Esp3I CGTCTC 1 cut(s) 11
FaeI CATG 3 cut(s) 1088, 1184, 1273
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FaqI GGGAC 1 cut(s) 155
FatI CATG 3 cut(s) 1084, 1180, 1269
FauI CCCGC 2 cut(s) 145, 260
Fnu4HI GCNGC 3 cut(s) 159, 308, 708
FokI GGATG 2 cut(s) 500, 1058
Fsp4HI GCNGC 3 cut(s) 159, 308, 708
FspBI CTAG 8 cut(s) 432, 647, 861, 963, 1041, 1130, 1278, 1310
GluI GCNGC 3 cut(s) 159, 308, 708
GsuI CTGGAG 1 cut(s) 1134
HaeIII GGCC 3 cut(s) 216, 307, 748
HapII CCGG 3 cut(s) 213, 243, 304
HgaI GACGC 1 cut(s) 94
Hin1II CATG 3 cut(s) 1088, 1184, 1273
HindIII AAGCTT 1 cut(s) 753
HinfI GANTC 9 cut(s) 80, 119, 153, 284, 568, 730, 1028, 1125, 1144
HpaII CCGG 3 cut(s) 213, 243, 304
HphI GGTGA 4 cut(s) 343, 482, 521, 899
Hpy166II GTNNAC 1 cut(s) 667
Hpy188I TCNGA 5 cut(s) 190, 573, 729, 1080, 1117
Hpy188III TCNNGA 7 cut(s) 116, 281, 392, 532, 796, 1151, 1190
Hpy8I GTNNAC 1 cut(s) 667
Hpy99I CGWCG 2 cut(s) 110, 323
HpyAV CCTTC 2 cut(s) 67, 1284
HpyCH4III ACNGT 1 cut(s) 1295
HpyCH4IV ACGT 1 cut(s) 972
HpyCH4V TGCA 2 cut(s) 667, 917
HpyF10VI GCNNNNNNNGC 4 cut(s) 243, 252, 842, 1270
HpyF3I CTNAG 5 cut(s) 233, 393, 698, 851, 999
HpySE526I ACGT 1 cut(s) 972
Hsp92II CATG 3 cut(s) 1088, 1184, 1273
KroI GCCGGC 1 cut(s) 303
KroNI GCCGGC 1 cut(s) 305
Kzo9I GATC 3 cut(s) 258, 1103, 1353
LmnI GCTCC 4 cut(s) 242, 715, 779, 1063
Lsp1109I GCAGC 1 cut(s) 694
LweI GCATC 4 cut(s) 255, 904, 1027, 1268
MaeI CTAG 8 cut(s) 432, 647, 861, 963, 1041, 1130, 1278, 1310
MaeII ACGT 1 cut(s) 972
MaeIII GTNAC 2 cut(s) 410, 968
MalI GATC 3 cut(s) 260, 1105, 1355
MbiI CCGCTC 1 cut(s) 310
MboI GATC 3 cut(s) 258, 1103, 1353
MboII GAAGA 2 cut(s) 340, 893
MflI RGATCY 1 cut(s) 1353
MhlI GDGCHC 1 cut(s) 669
MluCI AATT 9 cut(s) 421, 562, 624, 806, 923, 1053, 1107, 1237, 1339
MlyI GAGTC 4 cut(s) 74, 113, 162, 278
MmeI TCCRAC 4 cut(s) 154, 213, 707, 889
Mph1103I ATGCAT 1 cut(s) 919
MroNI GCCGGC 1 cut(s) 303
MroXI GAANNNNTTC 1 cut(s) 1295
MseI TTAA 5 cut(s) 35, 378, 549, 993, 1200
MspI CCGG 3 cut(s) 213, 243, 304
MspR9I CCNGG 2 cut(s) 243, 704
Mva1269I GAATGC 2 cut(s) 387, 561
MvaI CCWGG 1 cut(s) 704
MvnI CGCG 2 cut(s) 105, 329
MwoI GCNNNNNNNGC 4 cut(s) 243, 252, 842, 1270
NaeI GCCGGC 1 cut(s) 305
NciI CCSGG 1 cut(s) 243
NdeII GATC 3 cut(s) 258, 1103, 1353
NgoMIV GCCGGC 1 cut(s) 303
NheI GCTAGC 1 cut(s) 962
NlaIII CATG 3 cut(s) 1088, 1184, 1273
NlaIV GGNNCC 2 cut(s) 681, 946
NmuCI GTSAC 1 cut(s) 968
NsiI ATGCAT 1 cut(s) 919
NspI RCATGY 1 cut(s) 1088
NspV TTCGAA 1 cut(s) 27
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 2 cut(s) 387, 561
PdiI GCCGGC 1 cut(s) 305
PdmI GAANNNNTTC 1 cut(s) 1295
PfeI GAWTC 5 cut(s) 568, 730, 1028, 1125, 1144
PkrI GCNGC 3 cut(s) 160, 309, 709
PleI GAGTC 4 cut(s) 74, 113, 161, 278
PpsI GAGTC 4 cut(s) 74, 113, 161, 278
Psp6I CCWGG 1 cut(s) 702
PspGI CCWGG 1 cut(s) 702
PspN4I GGNNCC 2 cut(s) 681, 946
PspPI GGNCC 1 cut(s) 1177
PsuI RGATCY 1 cut(s) 1353
RsaI GTAC 1 cut(s) 893
RsaNI GTAC 1 cut(s) 892
SaqAI TTAA 5 cut(s) 35, 378, 549, 993, 1200
SatI GCNGC 3 cut(s) 159, 308, 708
Sau3AI GATC 3 cut(s) 258, 1103, 1353
Sau96I GGNCC 1 cut(s) 1177
SchI GAGTC 4 cut(s) 74, 113, 162, 278
ScrFI CCNGG 2 cut(s) 243, 704
SduI GDGCHC 1 cut(s) 669
SfaNI GCATC 4 cut(s) 255, 904, 1027, 1268
SfuI TTCGAA 1 cut(s) 27
SinI GGWCC 1 cut(s) 1177
SmlI CTYRAG 1 cut(s) 796
SmoI CTYRAG 1 cut(s) 796
Sse9I AATT 9 cut(s) 421, 562, 624, 806, 923, 1053, 1107, 1237, 1339
SsiI CCGC 9 cut(s) 103, 138, 159, 181, 253, 301, 308, 329, 865
SspMI CTAG 8 cut(s) 432, 647, 861, 963, 1041, 1130, 1278, 1310
StyD4I CCNGG 2 cut(s) 241, 702
TaaI ACNGT 1 cut(s) 1295
TaiI ACGT 1 cut(s) 975
TaqI TCGA 4 cut(s) 12, 27, 117, 1189
TasI AATT 9 cut(s) 421, 562, 624, 806, 923, 1053, 1107, 1237, 1339
TatI WGTACW 1 cut(s) 891
TauI GCSGC 2 cut(s) 161, 310
TfiI GAWTC 5 cut(s) 568, 730, 1028, 1125, 1144
Tru1I TTAA 5 cut(s) 35, 378, 549, 993, 1200
Tru9I TTAA 5 cut(s) 35, 378, 549, 993, 1200
TscAI CASTG 1 cut(s) 852
TseFI GTSAC 1 cut(s) 968
TseI GCWGC 1 cut(s) 707
Tsp45I GTSAC 1 cut(s) 968
TspGWI ACGGA 2 cut(s) 590, 1333
TspRI CASTG 1 cut(s) 852
VneI GTGCAC 1 cut(s) 665
VpaK11BI GGWCC 1 cut(s) 1177
XapI RAATTY 3 cut(s) 923, 1237, 1339
XceI RCATGY 1 cut(s) 1088
XmnI GAANNNNTTC 1 cut(s) 1295
XspI CTAG 8 cut(s) 432, 647, 861, 963, 1041, 1130, 1278, 1310
Zsp2I ATGCAT 1 cut(s) 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.