pycom07g05430

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001235
Physical Location & Seq
Reverse (-)
4736871 .. 4738295
1425 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g05430.1

Sequence Viewer

Length: 1425 bp
ATGGCTTTGGAGAAGAACACATTCGAAACCATAACCCCATCTCGTTTCATCACCTTCACTTTCCCAAACCCCTCCAACTCTTCCTCACTCCTCCGCGTCGCCGTCCTCGACTCACCTCTTCAACTCACTGACTCACCCCGAGTCGCCGCCATGCTAGTCCCCAACCACCGCGAGTCCGACTGGATATTCTCCACCGAGTCGGGCCACCTCCAGCTCCTTCTCAGCTCTCCCGGGATCTCCCGCCTAATCCTCATCGGCAGCAACCAATCCGCAGACGCTCACCATTCACCGCCAATCTACCACCGTCAGGATAAAAACGACACGTCGTGTGATGAGGAAGTCGGGGTGAGTTTGAAATTGAAACCTCTATTGCTTGCTTTATCTCCAAAGTGCTGCTTCAAATTTGGCATTCCTGAGATACCCATTTTGTGTTATGAAGATAATGTGGTTTCTAGTGTGGTTTTGGAGAGGTGTGTTGGGTCTCTGGTTGGGGAAATGGTGGTGGAAGATGTGGAGATTGAGAGTGGCGCCGAGGTTTCGAAGAGGGAGTTCAGGAGGCGTTTGAGGTTTAAGAGATTGCCCAATTTGGTTCAAACGGAGGTACGCATTGTTCCGAAGACGGGTTTTGGTTTTGATTGTGTGGAATTTGGAGAAATAGAGTTTAGGCTCGATAATAGTGTTTTAGTGCACCCTTATTTGGTTCCTATGGTAGCAAGTCTGAGTCTTGTTGGTTCTTGTGTCGAGGAACAGATTCGAAGTGGGTTTAGACCCAAAGCTTTGTGTTTAGGAGTTGGTGGTGGAGCTTTGCTTGGATTTTTAAGTGCCCATTTGGGTTTTCAGGTTATGGGTGTGGAGGCGGATGGGGAGGTTCTAAGGGTTGCTAAGAAGTATTTTGGACTGGAAGATGGTGAGCATATCAATGTTCACGTTGGGGATGCAATAAAGTTTATAGAGAAACTTGCTTTCCGTGTTAATGGACAGACTGCCGGTTCTATTTTTGCAGATGAGGTAGGGGATAGTAGTGAGTCTTGTGACATGGGCGATGGCAATGACGTTCATACTAAATTTGATGTAGTGCTTGTTGATTTAGATTCAAGTGATGCTGGGGATGGTATAATTGCTCCGCCGTTCGAGTTTGTTAGGAAGCATGTGCTTTTGGCTGCCAAATCAGTTCTTTGTGATAATGGCATCTTTGCTATAAATGTTATTCCTCCAAATCGGTCATTTTACACAACAATGATACGTGAGTTTAGAGAAGTTTTTCATGAGTTGTATGAAATAGACGTTGGAAATGGGGAAAATTTTATTCTTATTGCTGTTGTGTCACCCGTTACATCTGCTACTAGTGATTGTGAAAACTCTTTCCTTAACAAACTAAGAATGGTTGTATCAGGAGCATACTTGAATTCCATAAAGAAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synth

Protein Analysis

475

Amino Acids

52.0

Weight (kDa)

5.16

Isoelectric Point (pI)

44.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 527
AccII CGCG 2 cut(s) 96, 171
AciI CCGC 8 cut(s) 94, 147, 169, 241, 270, 290, 857, 1124
AclWI GGATC 1 cut(s) 242
AcsI RAATTY 5 cut(s) 401, 644, 1064, 1300, 1405
AcyI GRCGYC 1 cut(s) 528
AdeI CACNNNGTG 1 cut(s) 327
AfaI GTAC 1 cut(s) 603
AfiI CCNNNNNNNGG 3 cut(s) 307, 620, 697
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 7 cut(s) 122, 355, 361, 400, 593, 1095, 1405
AhlI ACTAGT 1 cut(s) 1343
AjiI CACGTC 1 cut(s) 324
AjuI GAANNNNNNNTTGG 2 cut(s) 1269, 1301
AluBI AGCT 4 cut(s) 214, 225, 776, 803
AluI AGCT 4 cut(s) 214, 225, 776, 803
Alw21I GWGCWC 1 cut(s) 690
Alw26I GTCTC 1 cut(s) 486
Alw44I GTGCAC 1 cut(s) 686
AlwI GGATC 1 cut(s) 242
Ama87I CYCGRG 2 cut(s) 138, 230
AoxI GGCC 1 cut(s) 202
ApaLI GTGCAC 1 cut(s) 686
ApeKI GCWGC 3 cut(s) 258, 393, 1160
ApoI RAATTY 5 cut(s) 401, 644, 1064, 1300, 1405
Asp700I GAANNNNTTC 3 cut(s) 20, 750, 1260
AspLEI GCGC 1 cut(s) 530
AspS9I GGNCC 1 cut(s) 202
AsuC2I CCSGG 2 cut(s) 231, 232
AsuHPI GGTGA 8 cut(s) 43, 105, 126, 272, 279, 358, 920, 1317
AsuII TTCGAA 3 cut(s) 24, 539, 754
AvaI CYCGRG 2 cut(s) 138, 230
BaeGI GKGCMC 2 cut(s) 690, 826
BaeI ACNNNNGTAYC 2 cut(s) 1232, 1265
BanI GGYRCC 1 cut(s) 527
BbsI GAAGAC 1 cut(s) 623
Bbv12I GWGCWC 1 cut(s) 690
BbvI GCAGC 3 cut(s) 270, 380, 1147
BccI CCATC 5 cut(s) 46, 854, 899, 1037, 1103
BceAI ACGGC 2 cut(s) 86, 1111
BcnI CCSGG 2 cut(s) 231, 232
BcoDI GTCTC 1 cut(s) 486
BcuI ACTAGT 1 cut(s) 1343
BfaI CTAG 3 cut(s) 155, 453, 1344
BfoI RGCGCY 1 cut(s) 531
BglII AGATCT 1 cut(s) 1419
BisI GCNGC 4 cut(s) 147, 259, 394, 1161
BlsI GCNGC 4 cut(s) 148, 260, 395, 1162
Bme1390I CCNGG 2 cut(s) 231, 232
BmeT110I CYCGRG 2 cut(s) 138, 230
BmgBI CACGTC 1 cut(s) 324
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 2 cut(s) 529, 702
BmrFI CCNGG 2 cut(s) 231, 232
BmsI GCATC 3 cut(s) 925, 1090, 1197
BpiI GAAGAC 1 cut(s) 623
BpmI CTGGAG 1 cut(s) 194
Bpu14I TTCGAA 3 cut(s) 24, 539, 754
BpuMI CCSGG 2 cut(s) 231, 232
BsaAI YACGTR 1 cut(s) 1244
BsaHI GRCGYC 1 cut(s) 528
BsaI GGTCTC 1 cut(s) 486
BsaJI CCNNGG 2 cut(s) 230, 531
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 620, 697
Bse118I RCCGGY 1 cut(s) 986
Bse1I ACTGG 2 cut(s) 185, 903
Bse3DI GCAATG 1 cut(s) 1054
BseDI CCNNGG 2 cut(s) 230, 531
BseGI GGATG 3 cut(s) 865, 940, 1114
BseLI CCNNNNNNNGG 3 cut(s) 307, 620, 697
BseMI GCAATG 1 cut(s) 1054
BseMII CTCAG 3 cut(s) 235, 405, 710
BseNI ACTGG 2 cut(s) 185, 903
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 2 cut(s) 690, 826
BseXI GCAGC 3 cut(s) 270, 380, 1147
BseYI CCCAGC 1 cut(s) 1103
Bsh1236I CGCG 2 cut(s) 96, 171
BshFI GGCC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 690
BsiHKCI CYCGRG 2 cut(s) 138, 230
BsiSI CCGG 2 cut(s) 231, 987
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 3 cut(s) 307, 620, 697
BsmAI GTCTC 1 cut(s) 486
BsmFI GGGAC 1 cut(s) 143
BsmI GAATGC 1 cut(s) 408
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 486
BsoBI CYCGRG 2 cut(s) 138, 230
Bsp119I TTCGAA 3 cut(s) 24, 539, 754
Bsp1286I GDGCHC 2 cut(s) 690, 826
Bsp143I GATC 2 cut(s) 234, 1419
BspACI CCGC 8 cut(s) 94, 147, 169, 241, 270, 290, 857, 1124
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 3 cut(s) 234, 406, 711
BspFNI CGCG 2 cut(s) 96, 171
BspHI TCATGA 1 cut(s) 1264
BspLI GGNNCC 2 cut(s) 529, 702
BspPI GGATC 1 cut(s) 242
BspT104I TTCGAA 3 cut(s) 24, 539, 754
BspT107I GGYRCC 1 cut(s) 527
BspTNI GGTCTC 1 cut(s) 486
BsrDI GCAATG 1 cut(s) 1054
BsrFI RCCGGY 1 cut(s) 986
BsrI ACTGG 2 cut(s) 185, 903
BssAI RCCGGY 1 cut(s) 986
BssECI CCNNGG 2 cut(s) 230, 531
BssMI GATC 2 cut(s) 234, 1419
BssNI GRCGYC 1 cut(s) 528
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 3 cut(s) 85, 123, 536
BstACI GRCGYC 1 cut(s) 528
BstBAI YACGTR 1 cut(s) 1244
BstBI TTCGAA 3 cut(s) 24, 539, 754
BstC8I GCNNGC 1 cut(s) 375
BstDEI CTNAG 6 cut(s) 221, 414, 719, 872, 882, 1376
BstF5I GGATG 3 cut(s) 865, 940, 1114
BstFNI CGCG 2 cut(s) 96, 171
BstH2I RGCGCY 1 cut(s) 531
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 2 cut(s) 237, 1422
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 2 cut(s) 234, 1419
BstNSI RCATGY 1 cut(s) 1151
BstSCI CCNGG 2 cut(s) 229, 230
BstSLI GKGCMC 2 cut(s) 690, 826
BstUI CGCG 2 cut(s) 96, 171
BstV1I GCAGC 3 cut(s) 270, 380, 1147
BstV2I GAAGAC 1 cut(s) 623
BstX2I RGATCY 2 cut(s) 234, 1419
BstYI RGATCY 2 cut(s) 234, 1419
BsuRI GGCC 1 cut(s) 204
BtgZI GCGATG 1 cut(s) 1056
BtrI CACGTC 1 cut(s) 324
BtsCI GGATG 3 cut(s) 865, 940, 1114
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 375
CciI TCATGA 1 cut(s) 1264
CfoI GCGC 1 cut(s) 530
Cfr10I RCCGGY 1 cut(s) 986
Cfr13I GGNCC 1 cut(s) 202
Cfr9I CCCGGG 1 cut(s) 230
CseI GACGC 2 cut(s) 85, 284
Csp6I GTAC 1 cut(s) 602
CviAII CATG 4 cut(s) 151, 1036, 1148, 1265
CviJI RGCY 8 cut(s) 5, 204, 214, 225, 667, 776, 803, 1160
CviKI_1 RGCY 8 cut(s) 5, 204, 214, 225, 667, 776, 803, 1160
CviQI GTAC 1 cut(s) 602
DdeI CTNAG 6 cut(s) 221, 414, 719, 872, 882, 1376
DinI GGCGCC 1 cut(s) 529
DpnI GATC 2 cut(s) 236, 1421
DpnII GATC 2 cut(s) 234, 1419
DraIII CACNNNGTG 1 cut(s) 327
Eam1104I CTCTTC 3 cut(s) 85, 123, 536
EarI CTCTTC 3 cut(s) 85, 123, 536
EciI GGCGGA 2 cut(s) 872, 1113
Eco31I GGTCTC 1 cut(s) 486
Eco88I CYCGRG 2 cut(s) 138, 230
EcoRI GAATTC 1 cut(s) 1405
EgeI GGCGCC 1 cut(s) 529
EheI GGCGCC 1 cut(s) 529
FaeI CATG 4 cut(s) 154, 1039, 1151, 1268
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FaqI GGGAC 1 cut(s) 143
FatI CATG 4 cut(s) 150, 1035, 1147, 1264
FauI CCCGC 1 cut(s) 248
Fnu4HI GCNGC 4 cut(s) 147, 259, 394, 1161
FokI GGATG 3 cut(s) 872, 947, 1121
Fsp4HI GCNGC 4 cut(s) 147, 259, 394, 1161
FspBI CTAG 3 cut(s) 155, 453, 1344
GlaI GCGC 1 cut(s) 529
GluI GCNGC 4 cut(s) 147, 259, 394, 1161
GsaI CCCAGC 1 cut(s) 1107
GsuI CTGGAG 1 cut(s) 194
HaeII RGCGCY 1 cut(s) 531
HaeIII GGCC 1 cut(s) 204
HapII CCGG 2 cut(s) 231, 987
HgaI GACGC 2 cut(s) 85, 284
HhaI GCGC 1 cut(s) 530
Hin1I GRCGYC 1 cut(s) 528
Hin1II CATG 4 cut(s) 154, 1039, 1151, 1268
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HindIII AAGCTT 1 cut(s) 774
HinfI GANTC 9 cut(s) 110, 131, 141, 173, 197, 721, 751, 1025, 1091
HpaII CCGG 2 cut(s) 231, 987
HphI GGTGA 8 cut(s) 43, 105, 126, 272, 279, 358, 920, 1317
Hpy166II GTNNAC 2 cut(s) 688, 925
Hpy188I TCNGA 3 cut(s) 178, 615, 720
Hpy188III TCNNGA 5 cut(s) 308, 413, 553, 1265, 1392
Hpy8I GTNNAC 2 cut(s) 688, 925
Hpy99I CGWCG 2 cut(s) 101, 328
HpyAV CCTTC 2 cut(s) 64, 227
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 5 cut(s) 323, 927, 1053, 1243, 1284
HpyCH4V TGCA 3 cut(s) 688, 938, 1001
HpyF3I CTNAG 6 cut(s) 221, 414, 719, 872, 882, 1376
HpySE526I ACGT 5 cut(s) 323, 927, 1053, 1243, 1284
Hsp92I GRCGYC 1 cut(s) 528
Hsp92II CATG 4 cut(s) 154, 1039, 1151, 1268
HspAI GCGC 1 cut(s) 528
KasI GGCGCC 1 cut(s) 527
Kzo9I GATC 2 cut(s) 234, 1419
LmnI GCTCC 4 cut(s) 219, 800, 1126, 1394
Lsp1109I GCAGC 3 cut(s) 270, 380, 1147
LweI GCATC 3 cut(s) 925, 1090, 1197
MaeI CTAG 3 cut(s) 155, 453, 1344
MaeII ACGT 5 cut(s) 323, 927, 1053, 1243, 1284
MaeIII GTNAC 3 cut(s) 1031, 1323, 1330
MalI GATC 2 cut(s) 236, 1421
MboI GATC 2 cut(s) 234, 1419
MboII GAAGA 8 cut(s) 25, 72, 110, 449, 518, 553, 628, 914
MflI RGATCY 2 cut(s) 234, 1419
MhlI GDGCHC 2 cut(s) 690, 826
MluCI AATT 8 cut(s) 356, 401, 583, 644, 1064, 1116, 1300, 1405
Mly113I GGCGCC 1 cut(s) 528
MlyI GAGTC 7 cut(s) 104, 125, 150, 182, 206, 730, 1034
MmeI TCCRAC 3 cut(s) 99, 201, 1267
MroXI GAANNNNTTC 3 cut(s) 20, 750, 1260
MseI TTAA 4 cut(s) 570, 818, 972, 1368
MslI CAYNNNNRTG 2 cut(s) 918, 1235
MspI CCGG 2 cut(s) 231, 987
MspR9I CCNGG 2 cut(s) 231, 232
Mva1269I GAATGC 1 cut(s) 408
MvnI CGCG 2 cut(s) 96, 171
NarI GGCGCC 1 cut(s) 528
NciI CCSGG 2 cut(s) 231, 232
NdeII GATC 2 cut(s) 234, 1419
NlaIII CATG 4 cut(s) 154, 1039, 1151, 1268
NlaIV GGNNCC 2 cut(s) 529, 702
NmeAIII GCCGAG 1 cut(s) 556
NmuCI GTSAC 2 cut(s) 1031, 1323
NspI RCATGY 1 cut(s) 1151
NspV TTCGAA 3 cut(s) 24, 539, 754
PagI TCATGA 1 cut(s) 1264
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 1 cut(s) 408
PdmI GAANNNNTTC 3 cut(s) 20, 750, 1260
PfeI GAWTC 2 cut(s) 751, 1091
PkrI GCNGC 4 cut(s) 148, 260, 395, 1162
PleI GAGTC 7 cut(s) 104, 125, 149, 181, 205, 729, 1033
PluTI GGCGCC 1 cut(s) 531
PpsI GAGTC 7 cut(s) 104, 125, 149, 181, 205, 729, 1033
Ppu21I YACGTR 1 cut(s) 1244
PspFI CCCAGC 1 cut(s) 1103
PspN4I GGNNCC 2 cut(s) 529, 702
PspPI GGNCC 1 cut(s) 202
PsrI GAACNNNNNNTAC 2 cut(s) 594, 626
PsuI RGATCY 2 cut(s) 234, 1419
RsaI GTAC 1 cut(s) 603
RsaNI GTAC 1 cut(s) 602
RseI CAYNNNNRTG 2 cut(s) 918, 1235
SaqAI TTAA 4 cut(s) 570, 818, 972, 1368
SatI GCNGC 4 cut(s) 147, 259, 394, 1161
Sau3AI GATC 2 cut(s) 234, 1419
Sau96I GGNCC 1 cut(s) 202
SchI GAGTC 7 cut(s) 104, 125, 150, 182, 206, 730, 1034
ScrFI CCNGG 2 cut(s) 231, 232
SduI GDGCHC 2 cut(s) 690, 826
SfaNI GCATC 3 cut(s) 925, 1090, 1197
SfoI GGCGCC 1 cut(s) 529
SfuI TTCGAA 3 cut(s) 24, 539, 754
SmaI CCCGGG 1 cut(s) 232
SmiMI CAYNNNNRTG 2 cut(s) 918, 1235
SpeI ACTAGT 1 cut(s) 1343
Sse9I AATT 8 cut(s) 356, 401, 583, 644, 1064, 1116, 1300, 1405
SsiI CCGC 8 cut(s) 94, 147, 169, 241, 270, 290, 857, 1124
SspDI GGCGCC 1 cut(s) 527
SspMI CTAG 3 cut(s) 155, 453, 1344
StyD4I CCNGG 2 cut(s) 229, 230
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 5 cut(s) 326, 930, 1056, 1246, 1287
TaqI TCGA 7 cut(s) 24, 108, 539, 669, 741, 754, 1131
TaqII GACCGA 1 cut(s) 1209
TasI AATT 8 cut(s) 356, 401, 583, 644, 1064, 1116, 1300, 1405
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 2 cut(s) 751, 1091
Tru1I TTAA 4 cut(s) 570, 818, 972, 1368
Tru9I TTAA 4 cut(s) 570, 818, 972, 1368
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 2 cut(s) 1031, 1323
TseI GCWGC 3 cut(s) 258, 393, 1160
Tsp45I GTSAC 2 cut(s) 1031, 1323
TspDTI ATGAA 5 cut(s) 37, 450, 1046, 1253, 1290
TspGWI ACGGA 2 cut(s) 611, 956
TspMI CCCGGG 1 cut(s) 230
TspRI CASTG 1 cut(s) 133
VneI GTGCAC 1 cut(s) 686
XapI RAATTY 5 cut(s) 401, 644, 1064, 1300, 1405
XceI RCATGY 1 cut(s) 1151
XmaI CCCGGG 1 cut(s) 230
XmnI GAANNNNTTC 3 cut(s) 20, 750, 1260
XspI CTAG 3 cut(s) 155, 453, 1344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.