Rroxscaffold_5G00352360

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001235
Physical Location & Seq
Reverse (-)
29352347 .. 29353179
833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00352360.1

Sequence Viewer

Length: 576 bp
ATGACTATCATCGATGAGACGACTTTCGAAATGTTAAACCCATCTCGTTTCACTACCTTCACTTTCCCAAACCCTTCCCACTCCACCACCGCCTCACTCCTCGCGTCGCCGTTCTCGACTCGCCTTCCCAACCTCCCCGCCGACCCACCCCGAGTTGCTTGCGGTGCTCGTCCCCAAACACCGCCACTCCGACCGGATATTCTCCACCCACTCCGCCATCTCCACCTCCTCCTCGGCTCCCCGGCATCTTCCGCCCGATCCTCGTCGGCGACAGCTCTCACGACTCGCCTCCAATCTACCGCGGTCCTCTCAAGACGACGAACTCGGGATGGGGAGCACATCAAAGTTTGTGTTGGAGATGCATTGGAGTTTTTAGATAAACCGGCTAGTGCTCGCGAGGTAGAGGCTAGGCGTGACGTGGGCAGAGCCAATGATGTTAATACTAAGTTTGATGTGATTATGTTTGATTTGGATTCAAGGGATGTTACGGATGGTCTAATTGCTTCCCCATTGGAGTTTGTTAAGAAGCATGTCCTTTTGTCGACGGATCAATTTTCCGTGATAATGGAATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synth

Protein Analysis

191

Amino Acids

21.0

Weight (kDa)

7.02

Isoelectric Point (pI)

58.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 542
AccII CGCG 3 cut(s) 104, 302, 396
AciI CCGC 8 cut(s) 90, 138, 162, 182, 214, 252, 300, 302
AclWI GGATC 2 cut(s) 252, 555
AgsI TTSAA 1 cut(s) 477
AjiI CACGTC 1 cut(s) 418
AluBI AGCT 1 cut(s) 275
AluI AGCT 1 cut(s) 275
Alw21I GWGCWC 3 cut(s) 169, 339, 394
Alw26I GTCTC 1 cut(s) 11
AlwI GGATC 2 cut(s) 252, 555
Ama87I CYCGRG 2 cut(s) 150, 324
AspS9I GGNCC 1 cut(s) 304
AsuC2I CCSGG 1 cut(s) 242
AsuII TTCGAA 1 cut(s) 27
AvaI CYCGRG 2 cut(s) 150, 324
AvaII GGWCC 1 cut(s) 304
Bbv12I GWGCWC 3 cut(s) 169, 339, 394
BccI CCATC 4 cut(s) 49, 225, 323, 485
BceAI ACGGC 1 cut(s) 94
BcgI CGANNNNNNTGC 2 cut(s) 141, 175
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 11
BfaI CTAG 3 cut(s) 387, 408, 574
Bme1390I CCNGG 1 cut(s) 242
Bme18I GGWCC 1 cut(s) 304
BmeT110I CYCGRG 2 cut(s) 150, 324
BmgBI CACGTC 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 304
BmiI GGNNCC 1 cut(s) 238
BmrFI CCNGG 1 cut(s) 242
BmsI GCATC 2 cut(s) 254, 349
Bpu14I TTCGAA 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 295
BpuMI CCSGG 1 cut(s) 242
Bsa29I ATCGAT 1 cut(s) 12
BsaJI CCNNGG 3 cut(s) 232, 240, 300
BsaWI WCCGGW 1 cut(s) 193
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bse118I RCCGGY 1 cut(s) 382
BseCI ATCGAT 1 cut(s) 12
BseDI CCNNGG 3 cut(s) 232, 240, 300
BseGI GGATG 3 cut(s) 334, 487, 496
BseRI GAGGAG 3 cut(s) 89, 218, 221
Bsh1236I CGCG 3 cut(s) 104, 302, 396
Bsh1285I CGRYCG 1 cut(s) 194
BshVI ATCGAT 1 cut(s) 12
BsiEI CGRYCG 1 cut(s) 194
BsiHKAI GWGCWC 3 cut(s) 169, 339, 394
BsiHKCI CYCGRG 2 cut(s) 150, 324
BsiSI CCGG 3 cut(s) 194, 242, 383
BslFI GGGAC 1 cut(s) 156
BsmAI GTCTC 1 cut(s) 11
BsmBI CGTCTC 1 cut(s) 11
BsmFI GGGAC 1 cut(s) 156
BsoBI CYCGRG 2 cut(s) 150, 324
Bsp119I TTCGAA 1 cut(s) 27
Bsp1286I GDGCHC 3 cut(s) 169, 339, 394
Bsp143I GATC 2 cut(s) 257, 547
Bsp68I TCGCGA 1 cut(s) 396
BspACI CCGC 8 cut(s) 90, 138, 162, 182, 214, 252, 300, 302
BspDI ATCGAT 1 cut(s) 12
BspFNI CGCG 3 cut(s) 104, 302, 396
BspLI GGNNCC 1 cut(s) 238
BspPI GGATC 2 cut(s) 252, 555
BspT104I TTCGAA 1 cut(s) 27
BsrFI RCCGGY 1 cut(s) 382
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 3 cut(s) 232, 240, 300
BssMI GATC 2 cut(s) 257, 547
BstBI TTCGAA 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 160, 394
BstDEI CTNAG 1 cut(s) 444
BstDSI CCRYGG 1 cut(s) 300
BstF5I GGATG 3 cut(s) 334, 487, 496
BstFNI CGCG 3 cut(s) 104, 302, 396
BstKTI GATC 2 cut(s) 260, 550
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 2 cut(s) 257, 547
BstMCI CGRYCG 1 cut(s) 194
BstMWI GCNNNNNNNGC 2 cut(s) 164, 251
BstNSI RCATGY 1 cut(s) 533
BstSCI CCNGG 1 cut(s) 240
BstUI CGCG 3 cut(s) 104, 302, 396
Bsu15I ATCGAT 1 cut(s) 12
BsuTUI ATCGAT 1 cut(s) 12
BtgI CCRYGG 1 cut(s) 300
BtrI CACGTC 1 cut(s) 418
BtsCI GGATG 3 cut(s) 334, 487, 496
BtuMI TCGCGA 1 cut(s) 396
Cac8I GCNNGC 2 cut(s) 160, 394
Cfr10I RCCGGY 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 304
Cfr42I CCGCGG 1 cut(s) 303
ClaI ATCGAT 1 cut(s) 12
CseI GACGC 1 cut(s) 93
CviAII CATG 1 cut(s) 530
CviJI RGCY 5 cut(s) 237, 275, 386, 407, 428
CviKI_1 RGCY 5 cut(s) 237, 275, 386, 407, 428
DdeI CTNAG 1 cut(s) 444
DpnI GATC 2 cut(s) 259, 549
DpnII GATC 2 cut(s) 257, 547
EciI GGCGGA 2 cut(s) 203, 241
Eco47I GGWCC 1 cut(s) 304
Eco88I CYCGRG 2 cut(s) 150, 324
EcoT22I ATGCAT 1 cut(s) 364
Esp3I CGTCTC 1 cut(s) 11
FaeI CATG 1 cut(s) 533
FaiI YATR 2 cut(s) 461, 531
FaqI GGGAC 1 cut(s) 156
FatI CATG 1 cut(s) 529
FauI CCCGC 1 cut(s) 145
FblI GTMKAC 1 cut(s) 542
FokI GGATG 3 cut(s) 341, 494, 503
FspBI CTAG 3 cut(s) 387, 408, 574
HapII CCGG 3 cut(s) 194, 242, 383
HgaI GACGC 1 cut(s) 93
Hin1II CATG 1 cut(s) 533
HincII GTYRAC 1 cut(s) 543
HindII GTYRAC 1 cut(s) 543
HinfI GANTC 4 cut(s) 118, 283, 473, 569
HpaII CCGG 3 cut(s) 194, 242, 383
Hpy166II GTNNAC 1 cut(s) 543
Hpy188I TCNGA 1 cut(s) 191
Hpy188III TCNNGA 5 cut(s) 115, 280, 312, 326, 395
Hpy8I GTNNAC 1 cut(s) 543
Hpy99I CGWCG 4 cut(s) 109, 268, 321, 547
HpyAV CCTTC 3 cut(s) 67, 84, 134
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 1 cut(s) 362
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 251
HpyF3I CTNAG 1 cut(s) 444
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 1 cut(s) 533
KspI CCGCGG 1 cut(s) 303
Kzo9I GATC 2 cut(s) 257, 547
LmnI GCTCC 2 cut(s) 242, 334
LpnPI CCDG 3 cut(s) 207, 255, 396
LweI GCATC 2 cut(s) 254, 349
MaeI CTAG 3 cut(s) 387, 408, 574
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 2 cut(s) 413, 484
MalI GATC 2 cut(s) 259, 549
MboI GATC 2 cut(s) 257, 547
MboII GAAGA 1 cut(s) 240
MhlI GDGCHC 3 cut(s) 169, 339, 394
MluCI AATT 2 cut(s) 498, 551
MlyI GAGTC 2 cut(s) 112, 277
MmeI TCCRAC 2 cut(s) 214, 334
Mph1103I ATGCAT 1 cut(s) 364
MseI TTAA 3 cut(s) 35, 438, 522
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 3 cut(s) 194, 242, 383
MspR9I CCNGG 1 cut(s) 242
MvnI CGCG 3 cut(s) 104, 302, 396
MwoI GCNNNNNNNGC 2 cut(s) 164, 251
NciI CCSGG 1 cut(s) 242
NdeII GATC 2 cut(s) 257, 547
NlaIII CATG 1 cut(s) 533
NlaIV GGNNCC 1 cut(s) 238
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 413
NruI TCGCGA 1 cut(s) 396
NsiI ATGCAT 1 cut(s) 364
NspI RCATGY 1 cut(s) 533
NspV TTCGAA 1 cut(s) 27
PcsI WCGNNNNNNNCGW 1 cut(s) 113
PfeI GAWTC 2 cut(s) 473, 569
PleI GAGTC 2 cut(s) 112, 277
PpsI GAGTC 2 cut(s) 112, 277
PspN4I GGNNCC 1 cut(s) 238
PspPI GGNCC 1 cut(s) 304
RruI TCGCGA 1 cut(s) 396
SacII CCGCGG 1 cut(s) 303
SalI GTCGAC 1 cut(s) 541
SaqAI TTAA 3 cut(s) 35, 438, 522
Sau3AI GATC 2 cut(s) 257, 547
Sau96I GGNCC 1 cut(s) 304
SchI GAGTC 2 cut(s) 112, 277
ScrFI CCNGG 1 cut(s) 242
SduI GDGCHC 3 cut(s) 169, 339, 394
SetI ASST 6 cut(s) 59, 135, 228, 277, 402, 420
SfaNI GCATC 2 cut(s) 254, 349
Sfr303I CCGCGG 1 cut(s) 303
SfuI TTCGAA 1 cut(s) 27
SgrBI CCGCGG 1 cut(s) 303
SinI GGWCC 1 cut(s) 304
SmlI CTYRAG 1 cut(s) 310
SmoI CTYRAG 1 cut(s) 310
Sse9I AATT 2 cut(s) 498, 551
SsiI CCGC 8 cut(s) 90, 138, 162, 182, 214, 252, 300, 302
SspMI CTAG 3 cut(s) 387, 408, 574
StyD4I CCNGG 1 cut(s) 240
TaiI ACGT 1 cut(s) 420
TaqI TCGA 4 cut(s) 12, 27, 116, 542
TasI AATT 2 cut(s) 498, 551
TfiI GAWTC 2 cut(s) 473, 569
Tru1I TTAA 3 cut(s) 35, 438, 522
Tru9I TTAA 3 cut(s) 35, 438, 522
TseFI GTSAC 1 cut(s) 413
Tsp45I GTSAC 1 cut(s) 413
TspGWI ACGGA 3 cut(s) 503, 547, 560
VpaK11BI GGWCC 1 cut(s) 304
XceI RCATGY 1 cut(s) 533
XmiI GTMKAC 1 cut(s) 542
XspI CTAG 3 cut(s) 387, 408, 574
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.