Rw0G013970

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00622
OG0001235
Physical Location & Seq
Forward (+)
70255 .. 71604
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G013970.1

Sequence Viewer

Length: 1269 bp
ATGGCTATCCTCGATAATACCACTTTCGAAATCATAAACCCATATCGTTTCATTACCTTTACGTTTCCAAACCCTTCGGACTCCACCACCTCACTCCTCCGCGTCGCCGTTCTCGACTCGCCTTTCCAACTCGCCGGCGACCCACCTCGAGTCGCCGGAATGCTCGTCCCTAAACACCGCCACTCCGACTGGATATTCTCCACCCACTCCGGCCATCTCCAGCTCCTCCTCAGCTCTCCCGGCATCTCCCGCCTAATTCTCATTGGCGACAGCTCTCACGACTCGCCGCCAATCTACCGCCGTCCTCTCAAAGACGACGCGTCGTGTGACGACGAACTCGGTGTGAGCCTGAAACCTCTCTTTCTTGCTTTATCTCCCAAGTCCTGCTTTAAACACGGCATTCCTGAGATACCCATTTTGAGTTACCAAGATAATTTGATTTCTAGTTTGGTTTTGGAGAAATGCGTTGGGTGTTTGGTTGGTGAAATGGTGGTGCAGGATGTGGAGATTGACTCTGGTGGTGAGGTTTCAAAGAGGGAGTTCAGGAGGCGTTTGAGGTTTGAAAGAATGCCGTATGTGATCCAGACGGAGGTGCGCATTGTTCCCAAAATGGGTTTTGGTTTGGATTGTGTGGGAATTGGACAAGTTGAGTTTAGGCTAGATGATAGTGTTTTAGTGCACTCTTATTTGGTTCCTATGGTCGCAGGTCTTAGCCTGGCTGCTTCTTACATTGACGGACGGATTCGAAGTCGGGTAAGGCCAAAAGCTTTGTGTTTAGGAGTTGGGGTGGGGCTTTGCTTGCCGATGAAGAGGTGCTCTAGGCGGTATTTTGGGCTAGACAATGGGGAGCATATCAAAGTTTGTGTTGGAGAAGTTATTGACAAACTTGCTGGTGCCCGTGAGGTAGAGGTTGGTTGTGACGTGGGCAGTGGTAATGATTATCAACATTTGATGGATGGTTTAATTGCTCCGCCATTGGAGTTTGTTTGGAAGCATATCCTTTTGTCAGCCAGATCAGTTCTTTCTGATTATGGAATCATAGCTATAAATGTGATTCCTCCAAATACATCATTTTACAGGACATTGATCCATGAGTTTCGAGATGTTTTCAATGAGCTTTACAAAATAGATGTGGGAAATGGAGAAAATTTTATTCTTATTGCTGTGGCATCTCCTCTCATGTCGTCTAGTAATTGTGAGAACTGTTTCCTCAGCAAACTAGGAAAAGCCATATCTGGGGCATACTTGAATTCCATAAAGAAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synth

Protein Analysis

422

Amino Acids

46.62

Weight (kDa)

6.6

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 596
Acc36I ACCTGC 1 cut(s) 695
AccB1I GGYRCC 1 cut(s) 893
AccII CGCG 2 cut(s) 102, 320
AciI CCGC 7 cut(s) 100, 178, 250, 287, 298, 823, 971
AclWI GGATC 2 cut(s) 574, 1081
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 2 cut(s) 1147, 1249
AfiI CCNNNNNNNGG 3 cut(s) 589, 611, 1236
AflIII ACRYGT 1 cut(s) 318
AgsI TTSAA 4 cut(s) 531, 563, 1111, 1249
AhdI GACNNNNNGTC 1 cut(s) 319
AjiI CACGTC 1 cut(s) 922
AjnI CCWGG 1 cut(s) 714
AluBI AGCT 6 cut(s) 223, 234, 273, 767, 1043, 1117
AluI AGCT 6 cut(s) 223, 234, 273, 767, 1043, 1117
Alw21I GWGCWC 2 cut(s) 681, 818
Alw44I GTGCAC 1 cut(s) 677
AlwI GGATC 2 cut(s) 574, 1081
Ama87I CYCGRG 1 cut(s) 147
AoxI GGCC 2 cut(s) 211, 758
ApaLI GTGCAC 1 cut(s) 677
ApeKI GCWGC 1 cut(s) 719
ApoI RAATTY 2 cut(s) 1147, 1249
Asp700I GAANNNNTTC 1 cut(s) 1205
AspLEI GCGC 1 cut(s) 597
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 2 cut(s) 494, 533
AsuII TTCGAA 2 cut(s) 27, 745
AvaI CYCGRG 1 cut(s) 147
BaeGI GKGCMC 2 cut(s) 681, 898
BanI GGYRCC 1 cut(s) 893
Bbv12I GWGCWC 2 cut(s) 681, 818
BbvCI CCTCAGC 2 cut(s) 230, 1211
BbvI GCAGC 1 cut(s) 706
BccI CCATC 3 cut(s) 222, 946, 950
BceAI ACGGC 4 cut(s) 92, 285, 412, 556
BciT130I CCWGG 1 cut(s) 716
BcnI CCSGG 1 cut(s) 240
BfaI CTAG 7 cut(s) 444, 659, 819, 836, 1188, 1220, 1267
BfuAI ACCTGC 1 cut(s) 695
BglII AGATCT 1 cut(s) 1263
BisI GCNGC 2 cut(s) 287, 720
BlsI GCNGC 2 cut(s) 288, 721
Bme1390I CCNGG 2 cut(s) 240, 716
BmeRI GACNNNNNGTC 1 cut(s) 319
BmeT110I CYCGRG 1 cut(s) 147
BmgBI CACGTC 1 cut(s) 922
BmiI GGNNCC 2 cut(s) 693, 895
BmrFI CCNGG 2 cut(s) 240, 716
BmsI GCATC 2 cut(s) 252, 1178
BplI GAGNNNNNCTC 2 cut(s) 497, 529
BpmI CTGGAG 1 cut(s) 203
Bpu10I CCTNAGC 2 cut(s) 230, 1211
Bpu14I TTCGAA 2 cut(s) 27, 745
BpuMI CCSGG 1 cut(s) 240
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 771, 801
Bsc4I CCNNNNNNNGG 3 cut(s) 589, 611, 1236
Bse118I RCCGGY 1 cut(s) 134
Bse1I ACTGG 1 cut(s) 194
BseBI CCWGG 1 cut(s) 716
BseGI GGATG 2 cut(s) 505, 961
BseLI CCNNNNNNNGG 3 cut(s) 589, 611, 1236
BseMII CTCAG 3 cut(s) 244, 396, 1225
BseNI ACTGG 1 cut(s) 194
BseRI GAGGAG 4 cut(s) 86, 215, 218, 1164
BseSI GKGCMC 2 cut(s) 681, 898
BseXI GCAGC 1 cut(s) 706
BsgI GTGCAG 1 cut(s) 515
Bsh1236I CGCG 2 cut(s) 102, 320
BshFI GGCC 2 cut(s) 213, 760
BshNI GGYRCC 1 cut(s) 893
BsiHKAI GWGCWC 2 cut(s) 681, 818
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 4 cut(s) 135, 156, 210, 240
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 3 cut(s) 589, 611, 1236
BsmFI GGGAC 1 cut(s) 152
BsmI GAATGC 3 cut(s) 165, 399, 573
BsnI GGCC 2 cut(s) 213, 760
BsoBI CYCGRG 1 cut(s) 147
Bsp119I TTCGAA 2 cut(s) 27, 745
Bsp1286I GDGCHC 3 cut(s) 681, 818, 898
Bsp143I GATC 4 cut(s) 579, 1013, 1086, 1263
BspACI CCGC 7 cut(s) 100, 178, 250, 287, 298, 823, 971
BspANI GGCC 2 cut(s) 213, 760
BspCNI CTCAG 3 cut(s) 243, 397, 1224
BspFNI CGCG 2 cut(s) 102, 320
BspLI GGNNCC 2 cut(s) 693, 895
BspMI ACCTGC 1 cut(s) 695
BspPI GGATC 2 cut(s) 574, 1081
BspT104I TTCGAA 2 cut(s) 27, 745
BspT107I GGYRCC 1 cut(s) 893
BsrFI RCCGGY 1 cut(s) 134
BsrI ACTGG 1 cut(s) 194
BssAI RCCGGY 1 cut(s) 134
BssMI GATC 4 cut(s) 579, 1013, 1086, 1263
Bst2UI CCWGG 1 cut(s) 716
Bst4CI ACNGT 1 cut(s) 1205
Bst6I CTCTTC 1 cut(s) 803
BstBI TTCGAA 2 cut(s) 27, 745
BstC8I GCNNGC 2 cut(s) 136, 800
BstDEI CTNAG 4 cut(s) 230, 405, 710, 1211
BstF5I GGATG 2 cut(s) 505, 961
BstFNI CGCG 2 cut(s) 102, 320
BstHHI GCGC 1 cut(s) 597
BstKTI GATC 4 cut(s) 582, 1016, 1089, 1266
BstMBI GATC 4 cut(s) 579, 1013, 1086, 1263
BstMWI GCNNNNNNNGC 3 cut(s) 240, 249, 799
BstNI CCWGG 1 cut(s) 716
BstSCI CCNGG 2 cut(s) 238, 714
BstSLI GKGCMC 2 cut(s) 681, 898
BstUI CGCG 2 cut(s) 102, 320
BstV1I GCAGC 1 cut(s) 706
BstX2I RGATCY 1 cut(s) 1263
BstYI RGATCY 1 cut(s) 1263
BsuRI GGCC 2 cut(s) 213, 760
BtrI CACGTC 1 cut(s) 922
BtsCI GGATG 2 cut(s) 505, 961
BtsI GCAGTG 1 cut(s) 934
BtsIMutI CAGTG 1 cut(s) 934
BveI ACCTGC 1 cut(s) 695
Cac8I GCNNGC 2 cut(s) 136, 800
CfoI GCGC 1 cut(s) 597
Cfr10I RCCGGY 1 cut(s) 134
CseI GACGC 3 cut(s) 91, 309, 326
CviAII CATG 2 cut(s) 1091, 1180
DdeI CTNAG 4 cut(s) 230, 405, 710, 1211
DpnI GATC 4 cut(s) 581, 1015, 1088, 1265
DpnII GATC 4 cut(s) 579, 1013, 1086, 1263
DraI TTTAAA 1 cut(s) 391
DriI GACNNNNNGTC 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 803
Eam1105I GACNNNNNGTC 1 cut(s) 319
EarI CTCTTC 1 cut(s) 803
EciI GGCGGA 1 cut(s) 960
Eco88I CYCGRG 1 cut(s) 147
EcoRI GAATTC 1 cut(s) 1249
EcoRII CCWGG 1 cut(s) 714
FaeI CATG 2 cut(s) 1094, 1183
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FaqI GGGAC 1 cut(s) 152
FatI CATG 2 cut(s) 1090, 1179
FauI CCCGC 1 cut(s) 257
Fnu4HI GCNGC 2 cut(s) 287, 720
FokI GGATG 2 cut(s) 512, 968
Fsp4HI GCNGC 2 cut(s) 287, 720
FspAI RTGCGCAY 1 cut(s) 596
FspBI CTAG 7 cut(s) 444, 659, 819, 836, 1188, 1220, 1267
FspI TGCGCA 1 cut(s) 596
GlaI GCGC 1 cut(s) 596
GluI GCNGC 2 cut(s) 287, 720
GsuI CTGGAG 1 cut(s) 203
HaeIII GGCC 2 cut(s) 213, 760
HapII CCGG 4 cut(s) 135, 156, 210, 240
HgaI GACGC 3 cut(s) 91, 309, 326
HhaI GCGC 1 cut(s) 597
Hin1II CATG 2 cut(s) 1094, 1183
Hin6I GCGC 1 cut(s) 595
HinP1I GCGC 1 cut(s) 595
HindIII AAGCTT 1 cut(s) 765
HinfI GANTC 8 cut(s) 80, 116, 150, 281, 512, 742, 1035, 1054
HpaII CCGG 4 cut(s) 135, 156, 210, 240
HphI GGTGA 2 cut(s) 494, 533
Hpy166II GTNNAC 1 cut(s) 679
Hpy188I TCNGA 3 cut(s) 79, 187, 1027
Hpy188III TCNNGA 6 cut(s) 113, 278, 404, 544, 583, 1100
Hpy8I GTNNAC 1 cut(s) 679
Hpy99I CGWCG 4 cut(s) 107, 320, 325, 335
HpyAV CCTTC 1 cut(s) 84
HpyCH4III ACNGT 1 cut(s) 1205
HpyCH4IV ACGT 2 cut(s) 62, 921
HpyCH4V TGCA 2 cut(s) 496, 679
HpyF10VI GCNNNNNNNGC 3 cut(s) 240, 249, 799
HpyF3I CTNAG 4 cut(s) 230, 405, 710, 1211
HpySE526I ACGT 2 cut(s) 62, 921
Hsp92II CATG 2 cut(s) 1094, 1183
HspAI GCGC 1 cut(s) 595
KroI GCCGGC 1 cut(s) 134
KroNI GCCGGC 1 cut(s) 136
Kzo9I GATC 4 cut(s) 579, 1013, 1086, 1263
LmnI GCTCC 3 cut(s) 228, 847, 973
Lsp1109I GCAGC 1 cut(s) 706
LweI GCATC 2 cut(s) 252, 1178
MaeI CTAG 7 cut(s) 444, 659, 819, 836, 1188, 1220, 1267
MaeII ACGT 2 cut(s) 62, 921
MaeIII GTNAC 3 cut(s) 326, 422, 917
MalI GATC 4 cut(s) 581, 1015, 1088, 1265
MboI GATC 4 cut(s) 579, 1013, 1086, 1263
MboII GAAGA 1 cut(s) 820
MflI RGATCY 1 cut(s) 1263
MhlI GDGCHC 3 cut(s) 681, 818, 898
MluCI AATT 7 cut(s) 255, 433, 636, 963, 1147, 1192, 1249
MluI ACGCGT 1 cut(s) 318
MlyI GAGTC 5 cut(s) 74, 110, 159, 275, 506
MmeI TCCRAC 3 cut(s) 151, 210, 847
MreI CGCCGGCG 1 cut(s) 134
MroNI GCCGGC 1 cut(s) 134
MroXI GAANNNNTTC 1 cut(s) 1205
MseI TTAA 2 cut(s) 390, 962
MspI CCGG 4 cut(s) 135, 156, 210, 240
MspR9I CCNGG 2 cut(s) 240, 716
Mva1269I GAATGC 3 cut(s) 165, 399, 573
MvaI CCWGG 1 cut(s) 716
MvnI CGCG 2 cut(s) 102, 320
MwoI GCNNNNNNNGC 3 cut(s) 240, 249, 799
NaeI GCCGGC 1 cut(s) 136
NciI CCSGG 1 cut(s) 240
NdeII GATC 4 cut(s) 579, 1013, 1086, 1263
NgoMIV GCCGGC 1 cut(s) 134
NlaIII CATG 2 cut(s) 1094, 1183
NlaIV GGNNCC 2 cut(s) 693, 895
NmuCI GTSAC 2 cut(s) 326, 917
NsbI TGCGCA 1 cut(s) 596
NspV TTCGAA 2 cut(s) 27, 745
PaeR7I CTCGAG 1 cut(s) 147
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 3 cut(s) 165, 399, 573
PdiI GCCGGC 1 cut(s) 136
PdmI GAANNNNTTC 1 cut(s) 1205
PfeI GAWTC 3 cut(s) 742, 1035, 1054
PkrI GCNGC 2 cut(s) 288, 721
PleI GAGTC 5 cut(s) 74, 110, 158, 275, 506
PpsI GAGTC 5 cut(s) 74, 110, 158, 275, 506
Psp6I CCWGG 1 cut(s) 714
PspGI CCWGG 1 cut(s) 714
PspN4I GGNNCC 2 cut(s) 693, 895
PspXI VCTCGAGB 1 cut(s) 147
PsuI RGATCY 1 cut(s) 1263
SaqAI TTAA 2 cut(s) 390, 962
SatI GCNGC 2 cut(s) 287, 720
Sau3AI GATC 4 cut(s) 579, 1013, 1086, 1263
SchI GAGTC 5 cut(s) 74, 110, 159, 275, 506
ScrFI CCNGG 2 cut(s) 240, 716
SduI GDGCHC 3 cut(s) 681, 818, 898
SfaNI GCATC 2 cut(s) 252, 1178
Sfr274I CTCGAG 1 cut(s) 147
SfuI TTCGAA 2 cut(s) 27, 745
SgrAI CRCCGGYG 1 cut(s) 134
SlaI CTCGAG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 147
SmoI CTYRAG 1 cut(s) 147
Sse9I AATT 7 cut(s) 255, 433, 636, 963, 1147, 1192, 1249
SsiI CCGC 7 cut(s) 100, 178, 250, 287, 298, 823, 971
SspMI CTAG 7 cut(s) 444, 659, 819, 836, 1188, 1220, 1267
StyD4I CCNGG 2 cut(s) 238, 714
TaaI ACNGT 1 cut(s) 1205
TaiI ACGT 2 cut(s) 65, 924
TaqI TCGA 6 cut(s) 12, 27, 114, 148, 745, 1099
TasI AATT 7 cut(s) 255, 433, 636, 963, 1147, 1192, 1249
TauI GCSGC 1 cut(s) 289
TfiI GAWTC 3 cut(s) 742, 1035, 1054
Tru1I TTAA 2 cut(s) 390, 962
Tru9I TTAA 2 cut(s) 390, 962
TscAI CASTG 1 cut(s) 934
TseFI GTSAC 2 cut(s) 326, 917
TseI GCWGC 1 cut(s) 719
Tsp45I GTSAC 2 cut(s) 326, 917
TspDTI ATGAA 2 cut(s) 40, 821
TspGWI ACGGA 3 cut(s) 602, 750, 754
TspRI CASTG 1 cut(s) 934
VneI GTGCAC 1 cut(s) 677
XapI RAATTY 2 cut(s) 1147, 1249
XhoI CTCGAG 1 cut(s) 147
XmnI GAANNNNTTC 1 cut(s) 1205
XspI CTAG 7 cut(s) 444, 659, 819, 836, 1188, 1220, 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.